SitesBLAST
Comparing WP_012709489.1 NCBI__GCF_000018545.1:WP_012709489.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8gqnA X-ray structure of thiolase with coa
59% identity, 98% coverage: 7:392/393 of query aligns to 3:390/390 of 8gqnA
6aqpA Aspergillus fumigatus cytosolic thiolase: acetylated enzyme in complex with coa and potassium ions
48% identity, 100% coverage: 1:392/393 of query aligns to 2:397/397 of 6aqpA
- active site: C93 (= C89), H353 (= H348), C383 (= C378), G385 (= G380)
- binding coenzyme a: C93 (= C89), L153 (= L149), Y188 (≠ L184), N226 (≠ K222), N228 (≠ D224), K231 (= K227), A248 (= A243), P249 (≠ A244), S252 (= S247), A323 (= A318), F324 (= F319), H353 (= H348)
Q4WCL5 Acetyl-CoA acetyltransferase erg10B, cytosolic; Acetoacetyl-CoA thiolase erg10B; ACAT; Cytosolic thiolase erg10B; CT; Ergosterol biosynthesis protein 10B; EC 2.3.1.9 from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata)
48% identity, 100% coverage: 1:392/393 of query aligns to 1:398/398 of Q4WCL5
- Y187 (≠ L184) binding K(+)
- N229 (≠ D224) binding CoA
- K232 (= K227) binding CoA
- A249 (= A243) binding K(+)
- P250 (≠ A244) binding K(+)
- S252 (= S246) binding K(+)
- S253 (= S247) binding CoA
- V350 (≠ C344) binding K(+)
- N385 (≠ I379) binding chloride
6aqpC Aspergillus fumigatus cytosolic thiolase: acetylated enzyme in complex with coa and potassium ions
48% identity, 100% coverage: 1:392/393 of query aligns to 2:399/399 of 6aqpC
- active site: C93 (= C89), H355 (= H348), C385 (= C378), G387 (= G380)
- binding acetyl coenzyme *a: C93 (= C89), L153 (= L149), M162 (= M158), Y188 (≠ L184), N230 (≠ D224), K233 (= K227), L234 (≠ I228), I237 (≠ L231), A250 (= A243), P251 (≠ A244), S254 (= S247), F295 (= F288), A325 (= A318), F326 (= F319), H355 (= H348)
P45359 Acetyl-CoA acetyltransferase; Acetoacetyl-CoA thiolase; CaTHL; EC 2.3.1.9 from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / IAM 19013 / LMG 5710 / NBRC 13948 / NRRL B-527 / VKM B-1787 / 2291 / W) (see paper)
47% identity, 98% coverage: 7:390/393 of query aligns to 4:390/392 of P45359
- V77 (≠ Q78) mutation to Q: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Y-153 and K-286.
- C88 (= C89) modified: Disulfide link with 378, In inhibited form
- S96 (≠ M97) binding acetate
- N153 (≠ S154) mutation to Y: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and K-286.
- GS 279:280 (≠ AG 279:280) binding acetate
- A286 (= A286) mutation to K: 3-fold increase in thiolase activity, prevents disulfide bond formation under oxidized condition and results in the loss of regulatory mechanism based on redox-switch modulation; when associated with Q-77 and Y-153.
- C378 (= C378) modified: Disulfide link with 88, In inhibited form
- A386 (= A386) binding acetate
4xl4A Crystal structure of thiolase from clostridium acetobutylicum in complex with coa (see paper)
46% identity, 98% coverage: 7:390/393 of query aligns to 4:390/392 of 4xl4A
- active site: C88 (= C89), H348 (= H348), S378 (≠ C378), G380 (= G380)
- binding coenzyme a: L148 (= L149), H156 (≠ L157), R220 (≠ T221), L231 (= L231), A243 (= A243), S247 (= S247), F319 (= F319), H348 (= H348)
6bn2A Crystal structure of acetyl-coa acetyltransferase from elizabethkingia anophelis nuhp1
45% identity, 98% coverage: 7:390/393 of query aligns to 4:390/393 of 6bn2A
2vu1A Biosynthetic thiolase from z. Ramigera. Complex of with o-pantheteine- 11-pivalate. (see paper)
47% identity, 98% coverage: 7:390/393 of query aligns to 4:389/391 of 2vu1A
1ou6A Biosynthetic thiolase from zoogloea ramigera in complex with acetyl-o- pantetheine-11-pivalate
47% identity, 98% coverage: 7:390/393 of query aligns to 5:390/392 of 1ou6A
- active site: C89 (= C89), H348 (= H348), C378 (= C378), G380 (= G380)
- binding pantothenyl-aminoethanol-acetate pivalic acid: L148 (= L149), H156 (≠ L157), M157 (= M158), F235 (= F235), A243 (= A243), S247 (= S247), A318 (= A318), F319 (= F319), H348 (= H348)
2vu2A Biosynthetic thiolase from z. Ramigera. Complex with s-pantetheine-11- pivalate. (see paper)
47% identity, 98% coverage: 7:390/393 of query aligns to 2:387/389 of 2vu2A
- active site: C86 (= C89), H345 (= H348), C375 (= C378), G377 (= G380)
- binding (3R)-3-hydroxy-2,2-dimethyl-4-oxo-4-({3-oxo-3-[(2-sulfanylethyl)amino]propyl}amino)butyl 2,2-dimethylpropanoate: H153 (≠ L157), M154 (= M158), F232 (= F235), S244 (= S247), G245 (≠ S248), F316 (= F319), H345 (= H348)
1dm3A Acetylated biosynthetic thiolase from zoogloea ramigera in complex with acetyl-coa (see paper)
47% identity, 98% coverage: 7:390/393 of query aligns to 2:387/389 of 1dm3A
- active site: C86 (= C89), H345 (= H348), C375 (= C378), G377 (= G380)
- binding acetyl coenzyme *a: C86 (= C89), L145 (= L149), H153 (≠ L157), M154 (= M158), R217 (≠ T221), S224 (≠ K227), M225 (≠ I228), A240 (= A243), S244 (= S247), M285 (≠ F288), A315 (= A318), F316 (= F319), H345 (= H348), C375 (= C378)
1dlvA Biosynthetic thiolase from zoogloea ramigera in complex with coa (see paper)
47% identity, 98% coverage: 7:390/393 of query aligns to 2:387/389 of 1dlvA