SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012709876.1 NCBI__GCF_000018545.1:WP_012709876.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 2 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
47% identity, 83% coverage: 4:92/107 of query aligns to 112:200/200 of 6j2lA

query
sites
6j2lA

L

L

T

F

L

L

S

Y

D

Q

L

L

E

E

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Q

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L

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L

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G

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E

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E

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L

A

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A

A

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D

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E

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A

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D

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Y

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L

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binding zinc ion: E152 (= E44), E171 (= E63), D174 (= D66)

Sites not aligning to the query:

binding zinc ion: 79, 81, 83, 96, 103

6j2lB Crystal structure of bi-functional enzyme (see paper)
40% identity, 83% coverage: 4:92/107 of query aligns to 105:185/185 of 6j2lB

query
sites
6j2lB

L

L

T

F

L

L

S

Y

D

Q

L

L

E

E

Q

Q

I

L

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L

A

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G

-

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G

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E

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D

L

L

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Y

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L

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L

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L

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L

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R

binding magnesium ion: E137 (= E44), E140 (= E47), E156 (= E63), D159 (= D66)

Sites not aligning to the query:

binding zinc ion: 78, 80, 82

Query Sequence

>WP_012709876.1 NCBI__GCF_000018545.1:WP_012709876.1
MTELTLSDLEQIVATRAGAAPEDSWTAKLVAAGQPKAAKKLGEEAVETVIAAVSGDRKNL
IDESADLLYHLMVVLNIAAVPLQDVMSELARRTSQSGLQEKANRQNP

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory