SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012709948.1 NCBI__GCF_000018545.1:WP_012709948.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4z0pA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
91% identity, 99% coverage: 4:319/319 of query aligns to 1:316/316 of 4z0pA

query
sites
4z0pA

K

K

S

S

P

P

V

V

I

I

V

V

D

D

L

L

K

K

F

F

I

I

P

P

E

E

V

V

D

E

A

A

L

L

N

A

G

G

A

A

F

F

P

P

G

G

R

R

E

E

V

V

I

I

N

D

L

L

A

A

D

D

P

P

V

A

H

H

K

Q

G

E

R

R

D

D

L

L

S

S

G

G

I

I

D

D

Y

Y

A

A

V

V

W
|
W

K

K

S

S

A

A

P

P

D

D

L

L

F

F

S

S

R

R

A

A

P

P

D

D

L

L

K

K

V

V

F

F

S

S

G

G

G
|
G

A
|
A

G
|
G

V

V

D

D

H

H

V

V

L

L

K

T

L

L

P

P

G

G

L

L

P

P

D

D

V

V

P

P

L

L

V

V

R
|
R

F

F

V

V

D

D

R

R

T

T

L
|
L

T

T

R

R

M
|
M

S

S

E

E

W

W

V

V

V

M

M

M

Q

Q

C

C

L

L

H

H

L

L

R

R

Q

Q

H
|
H

R
|
R

A

A

Y

Y

E

E

A

A

L

L

V

A

K

K

H

H

E

E

W

W

R

R

D

D

L

L

S

S

Q

Q

P

P

E

E

A

A

D

D

I

V

T

T

V

V

G

G

I

I

M

M

G

G

M
|
M

G
|
G

V
|
V

L
|
L

G

G

Q

Q

D

D

A

A

R

R

K

K

L

L

A

A

V

A

M

M

G

G

F

F

K

K

V

V

I

I

G

G

W
|
W

S
|
S

R
|
R

S
|
S

K

K

R
|
R

A

V

V

I

E

E

G

G

I

V

E

E

T

T

F

Y

D

D

A

A

G

G

L

L

D

D

A

A

F

F

L

L

A

G

R

R

T

T

D

D

F

F

L

L

V

V

G

G

L

L

L
|
L

P
|
P

L

L

T

T

A

P

D

D

T

T

K

R

G

G

I

I

F

F

N

N

A

A

T

A

L

L

F

F

K

A

K

K

L

L

S

S

R

R

K

N

G

G

P

P

F

F

G

G

A

A

P

P

V

V

F

F

I

I

N

N

A
|
A

G
|
G

R
|
R

G

G

S

S

Q

Q

V

V

E

E

A

A

D

D

I

I

L

L

E

E

C

C

L

L

D

D

S

S

G

G

V

V

L

L

G
|
G

G

G

A

A

S

S

L

L

D
|
D

V

V

F

F

E

E

K

R

E
|
E

P

P

L

L

S

S

R

P

E

E

S

S

R

R

F

F

W

W

D

D

I

M

P

P

N

N

V

V

Y

Y

V

V

T

T

P

P

H
|
H

V

V

A
|
A

S

S

D

D

V

V

K

R

A

A

L

L

F

F

A

V

H

H

V

V

E

E

E

H

Q

Q

I

I

E

A

R

R

F

F

E

E

S

S

G

G

L

L

A

P

L

L

E

E

H

H

V

V

D

D

K

K

V

V

A

A

G

G

Y
|
Y

active site: L95 (= L98), R231 (= R234), G250 (= G253), D255 (= D258), E260 (= E263), H279 (= H282)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (= R92), M99 (= M102), M144 (= M147), G145 (= G148), V146 (= V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), S168 (= S171), R170 (= R173), L197 (= L200), P198 (= P201), A229 (= A232), G230 (= G233), R231 (= R234), H279 (= H282), A281 (= A284), A282 (= A285), Y316 (= Y319)
binding oxalic acid: W50 (= W53), G70 (= G73), A71 (= A74), G72 (= G75), H114 (= H117), R115 (= R118), R231 (= R234), H279 (= H282)

4weqA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc02828 (smghra) from sinorhizobium meliloti in complex with NADP and sulfate (see paper)
91% identity, 99% coverage: 4:319/319 of query aligns to 1:316/316 of 4weqA

query
sites
4weqA

K

K

S

S

P

P

V

V

I

I

V

V

D

D

L

L

K

K

F

F

I

I

P

P

E

E

V

V

D

E

A

A

L

L

N

A

G

G

A

A

F

F

P

P

G

G

R

R

E

E

V

V

I

I

N

D

L

L

A

A

D

D

P

P

V

A

H

H

K

Q

G

E

R

R

D

D

L

L

S

S

G

G

I

I

D

D

Y

Y

A

A

V

V

W

W

K

K

S

S

A

A

P

P

D

D

L

L

F

F

S

S

R

R

A

A

P

P

D

D

L

L

K

K

V

V

F

F

S

S

G

G

A

A

G

G

V

V

D

D

H

H

V

V

L

L

K

T

L

L

P

P

G

G

L

L

P

P

D

D

V

V

P

P

L

L

V

V

R
|
R

F

F

V

V

D

D

R

R

T

T

L
|
L

T

T

R

R

M
|
M

S

S

E

E

W

W

V

V

V

M

M

M

Q

Q

C

C

L

L

H

H

L

L

R

R

Q

Q

H

H

R

R

A

A

Y

Y

E

E

A

A

L

L

V

A

K

K

H

H

E

E

W

W

R

R

D

D

L

L

S

S

Q

Q

P

P

E

E

A

A

D

D

I

V

T

T

V

V

G

G

I

I

M

M

G

G

M
|
M

G
|
G

V
|
V

L
|
L

G

G

Q

Q

D

D

A

A

R

R

K

K

L

L

A

A

V

A

M

M

G

G

F

F

K

K

V

V

I

I

G

G

W
|
W

S
|
S

R
|
R

S
|
S

K

K

R
|
R

A

V

V

I

E

E

G

G

I

V

E

E

T

T

F

Y

D

D

A

A

G

G

L

L

D

D

A

A

F

F

L

L

A

G

R

R

T

T

D

D

F

F

L

L

V

V

G

G

L

L

L
|
L

P
|
P

L

L

T

T

A

P

D

D

T

T

K

R

G

G

I

I

F

F

N

N

A

A

T

A

L

L

F

F

K

A

K

K

L

L

S

S

R

R

K

N

G

G

P

P

F

F

G

G

A

A

P

P

V

V

F

F

I

I

N

N

A
|
A

G
|
G

R
|
R

G

G

S

S

Q

Q

V

V

E

E

A

A

D

D

I

I

L

L

E

E

C

C

L

L

D

D

S

S

G

G

V

V

L

L

G
|
G

G

G

A

A

S

S

L

L

D
|
D

V

V

F

F

E

E

K

R

E
|
E

P

P

L

L

S

S

R

P

E

E

S

S

R

R

F

F

W

W

D

D

I

M

P

P

N

N

V

V

Y

Y

V

V

T

T

P

P

H
|
H

V

V

A
|
A

A

A

S

S

D

D

V

V

K

R

A

A

L

L

F

F

A

V

H

H

V

V

E

E

E

H

Q

Q

I

I

E

A

R

R

F

F

E

E

S

S

G

G

L

L

A

P

L

L

E

E

H

H

V

V

D

D

K

K

V

V

A

A

G

G

Y
|
Y

active site: L95 (= L98), R231 (= R234), G250 (= G253), D255 (= D258), E260 (= E263), H279 (= H282)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R89 (= R92), M99 (= M102), M144 (= M147), G145 (= G148), V146 (= V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), S168 (= S171), R170 (= R173), L197 (= L200), P198 (= P201), A229 (= A232), G230 (= G233), R231 (= R234), D255 (= D258), H279 (= H282), A281 (= A284), Y316 (= Y319)

5bqfA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADP, hepes and l(+)-tartaric acid
60% identity, 99% coverage: 4:319/319 of query aligns to 2:317/317 of 5bqfA

query
sites
5bqfA

K

R

S

P

P

P

V

V

I

L

V

V

D

D

L

I

K

K

F

F

I

N

P

P

E

E

E

G

V

V

D

D

A

R

A

V

L

L

N

K

G

T

A

A

F

F

P

A

G

D

R

R

E

G

V

S

I

I

N

N

L

L

A

A

D

D

P

P

V

A

H

N

K

R

G

E

R

R

D

D

L

F

S

S

G

E

I

T

D

E

Y

Y

A

A

V

L

W

W

K

K

S

P

A

D

P

A

D

D

L

L

F

F

S

R

R

R

A

A

P

P

D

N

L

L

K

K

V

V

V

I

F

F

S

S

G

G

A
|
A

G

G

V

V

D

D

H

H

V

I

L

I

K

G

L

M

P

A

G

G

L

L

P

P

D

D

V

I

P

P

L

I

V

V

R
|
R

F

F

V

V

D

D

R

R

T

S

L

L

T

T

R

R

M
|
M

S

S

E

E

W

W

V

V

M

M

Q

Q

C

C

L

L

L

M

H

H

L

L

R

R

Q

G

H

Q

R

Y

A

G

Y

H

E

D

A

S

L

H

V

Q

K

R

H

R

E

E

W

W

R

A

D

K

L

L

S

I

Q

A

P

P

E

E

A

A

D

E

I

V

T

T

V

V

G

G

I

V

M

M

G
|
G

M
x
L

G
|
G

V
x
I

L
|
L

G

G

Q

Q

D

D

A

A

A

V

R

A

K

K

L

L

A

K

V

V

M

M

G

G

F

F

K

N

V

V

I

I

G

G

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R

K

A

T

V

I

E

E

G

G

I

V

E

E

T

T

F

F

D

D

A

A

A

G

G

E

L

L

D

D

A

R

F

F

L

L

A

A

R

K

T

T

D

D

F

I

L

L

V

V

G

G

L

L

L
|
L

P
|
P

L

L

T

T

A

P

D

E

T

T

K

T

G

G

I

F

F

Y

N

D

A

S

T

E

L

L

F

F

K

K

L

L

S

R

R

R

K

D

G

G

P

A

F

L

G

G

A

Q

P

P

V

V

F

F

I

I

N

N

A
|
A

G
|
G

R
|
R

G

G

G

K

S

S

Q

Q

V

I

E

E

A

T

D

D

I

I

L

V

E

S

C

A

L

V

D

R

S

E

G

G

V

T

L

L

G

G

A

A

S

S

L

L

D

D

V

V

F

F

E

E

K

V

E
|
E

P

P

L

L

S

A

R

T

E

D

S

S

R

P

F

L

W

W

D

E

I

L

P

E

N

N

V

V

Y

F

V

I

T

T

P

P

H
|
H

V

D

A

A

A
|
A

S

V

S

S

D

E

V

E

K

N

A

A

L

L

F

F

A

R

H

H

V

V

E

E

E

M

Q

Q

I

I

E

A

R

R

F

F

E

E

S

R

G

G

L

E

A

P

L

L

E

Q

H

F

V

V

V

I

D

D

K

R

V

A

A

A

G

G

Y
|
Y

active site: E261 (= E263), H280 (= H282)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A72 (= A74), R90 (= R92), M100 (= M102), G144 (= G146), L145 (≠ M147), G146 (= G148), I147 (≠ V149), L148 (= L150), W166 (= W168), S167 (= S169), R168 (= R170), T169 (≠ S171), L198 (= L200), P199 (= P201), A230 (= A232), G231 (= G233), R232 (= R234), H280 (= H282), A283 (= A285), Y317 (= Y319)

4xcvA Probable 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH
60% identity, 99% coverage: 4:319/319 of query aligns to 2:317/317 of 4xcvA

query
sites
4xcvA

K

R

S

P

P

P

V

V

I

L

V

V

D

D

L

I

K

K

F

F

I

N

P

P

E

E

E

G

V

V

D

D

A

R

A

V

L

L

N

K

G

T

A

A

F

F

P

A

G

D

R

R

E

G

V

S

I

I

N

N

L

L

A

A

D

D

P

P

V

A

H

N

K

R

G

E

R

R

D

D

L

F

S

S

G

E

I

T

D

E

Y

Y

A

A

V

L

W

W

K

K

S

P

A

D

P

A

D

D

L

L

F

F

S

R

R

R

A

A

P

P

D

N

L

L

K

K

V

V

V

I

F

F

S

S

G

G

A
|
A

G

G

V

V

D

D

H

H

V

I

L

I

K

G

L

M

P

A

G

G

L

L

P

P

D

D

V

I

P

P

L

I

V

V

R
|
R

F

F

V

V

D

D

R

R

T

S

L

L

T

T

R

R

M
|
M

S

S

E

E

W

W

V

V

M

M

Q

Q

C

C

L

L

L

M

H

H

L

L

R

R

Q

G

H

Q

R

Y

A

G

Y

H

E

D

A

S

L

H

V

Q

K

R

H

R

E

E

W

W

R

A

D

K

L

L

S

I

Q

A

P

P

E

E

A

A

D

E

I

V

T

T

V

V

G

G

I

V

M

M

G
|
G

M
x
L

G
|
G

V
x
I

L
|
L

G

G

Q

Q

D

D

A

A

A

V

R

A

K

K

L

L

A

K

V

V

M

M

G

G

F

F

K

N

V

V

I

I

G

G

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R
x
K

A

T

V

I

E

E

G

G

I

V

E

E

T

T

F

F

D

D

A

A

A

G

G

E

L

L

D

D

A

R

F

F

L

L

A

A

R

K

T

T

D

D

F

I

L

L

V

V

G

G

L

L

L
|
L

P
|
P

L

L

T

T

A

P

D

E

T

T

K

T

G

G

I

F

F

Y

N

D

A

S

T

E

L

L

F

F

K

K

L

L

S

R

R

R

K

D

G

G

P

A

F

L

G

G

A

Q

P

P

V

V

F

F

I

I

N

N

A
|
A

G
|
G

R
|
R

G

G

G

K

S

S

Q

Q

V

I

E

E

A

T

D

D

I

I

L

V

E

S

C

A

L

V

D

R

S

E

G

G

V

T

L

L

G

G

A

A

S

S

L

L

D

D

V

V

F

F

E

E

K

V

E
|
E

P

P

L

L

S

A

R

T

E

D

S

S

R

P

F

L

W

W

D

E

I

L

P

E

N

N

V

V

Y

F

V

I

T

T

P

P

H
|
H

V

D

A

A

A
|
A

S

V

S

S

D

E

V

E

K

N

A

A

L

L

F

F

A

R

H

H

V

V

E

E

E

M

Q

Q

I

I

E

A

R

R

F

F

E

E

S

R

G

G

L

E

A

P

L

L

E

Q

H

F

V

V

V

I

D

D

K

R

V

A

A

A

G

G

Y
|
Y

active site: E261 (= E263), H280 (= H282)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A72 (= A74), R90 (= R92), M100 (= M102), G144 (= G146), L145 (≠ M147), G146 (= G148), I147 (≠ V149), L148 (= L150), W166 (= W168), S167 (= S169), R168 (= R170), T169 (≠ S171), K171 (≠ R173), L198 (= L200), P199 (= P201), A230 (= A232), G231 (= G233), R232 (= R234), H280 (= H282), A283 (= A285), Y317 (= Y319)

5tsdA Crystal structure of NADPH-dependent 2-hydroxyacid dehydrogenase from rhizobium etli cfn 42 in complex with NADPH and oxalate
60% identity, 99% coverage: 4:319/319 of query aligns to 1:316/316 of 5tsdA

query
sites
5tsdA

K

R

S

P

P

P

V

V

I

L

V

V

D

D

L

I

K

K

F

F

I

N

P

P

E

E

E

G

V

V

D

D

A

R

A

V

L

L

N

K

G

T

A

A

F

F

P

A

G

D

R

R

E

G

V

S

I

I

N

N

L

L

A

A

D

D

P

P

V

A

H

N

K

R

G

E

R

R

D

D

L

F

S

S

G

E

I

T

D

E

Y

Y

A

A

V

L

W
|
W

K

K

S

P

A

D

P

A

D

D

L

L

F

F

S

R

R

R

A

A

P

P

D

N

L

L

K

K

V

V

V

I

F

F

S

S

G

G

G
|
G

A
|
A

G
|
G

V

V

D

D

H

H

V

I

L

I

K

G

L

M

P

A

G

G

L

L

P

P

D

D

V

I

P

P

L

I

V

V

R
|
R

F

F

V

V

D

D

R

R

T

S

L

L

T

T

R

R

M
|
M

S

S

E

E

W

W

V

V

M

M

Q

Q

C

C

L

L

L

M

H

H

L

L

R

R

Q

G

H

Q

R

Y

A

G

Y

H

E

D

A

S

L

H

V

Q

K

R

H

R

E

E

W

W

R

A

D

K

L

L

S

I

Q

A

P

P

E

E

A

A

D

E

I

V

T

T

V

V

G

G

I

V

M

M

G
|
G

M
x
L

G
|
G

V
x
I

L
|
L

G

G

Q

Q

D

D

A

A

A

V

R

A

K

K

L

L

A

K

V

V

M

M

G

G

F

F

K

N

V

V

I

I

G

G

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R
x
K

A

T

V

I

E

E

G

G

I

V

E

E

T

T

F

F

D

D

A

A

A

G

G

E

L

L

D

D

A

R

F

F

L

L

A

A

R

K

T

T

D

D

F

I

L

L

V

V

G

G

L

L

L
|
L

P
|
P

L

L

T

T

A

P

D

E

T

T

K

T

G

G

I

F

F

Y

N

D

A

S

T

E

L

L

F

F

K

K

L

L

S

R

R

R

K

D

G

G

P

A

F

L

G

G

A

Q

P

P

V

V

F

F

I

I

N

N

A
|
A

G
|
G

R
|
R

G

G

G

K

S

S

Q

Q

V

I

E

E

A

T

D

D

I

I

L

V

E

S

C

A

L

V

D

R

S

E

G

G

V

T

L

L

G

G

A

A

S

S

L

L

D
|
D

V

V

F

F

E

E

K

V

E
|
E

P

P

L

L

S

A

R

T

E

D

S

S

R

P

F

L

W

W

D

E

I

L

P

E

N

N

V

V

Y

F

V

I

T

T

P

P

H
|
H

V

D

A

A

S

V

S

S

D

E

V

E

K

N

A

A

L

L

F

F

A

R

H

H

V

V

E

E

E

M

Q

Q

I

I

E

A

R

R

F

F

E

E

S

R

G

G

L

E

A

P

L

L

E

Q

H

F

V

V

V

I

D

D

K

R

V

A

A

A

G

G

Y
|
Y

active site: E260 (= E263), H279 (= H282)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A71 (= A74), R89 (= R92), M99 (= M102), G143 (= G146), L144 (≠ M147), G145 (= G148), I146 (≠ V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), T168 (≠ S171), K170 (≠ R173), L197 (= L200), P198 (= P201), A229 (= A232), G230 (= G233), R231 (= R234), D255 (= D258), H279 (= H282), Y316 (= Y319)
binding oxalic acid: W50 (= W53), G70 (= G73), A71 (= A74), G72 (= G75), R231 (= R234), H279 (= H282)

5vg6B Crystal structure of d-isomer specific 2-hydroxyacid dehydrogenase from xanthobacter autotrophicus py2 in complex with NADPH and mes.
38% identity, 96% coverage: 14:319/319 of query aligns to 13:315/315 of 5vg6B

query
sites
5vg6B

I

I

P

G

E

Q

E

D

V

W

D

K

A

S

A

A

L

L

N

Q

G

A

A

A

F

H

P

P

G

G

R

L

E

D

V

V

-

R

I

I

N

A

L

R

A

A

D

G

P

D

V

G

H

H

K

V

G

E

R

G

D

D

L

P

S

E

G

E

I

V

D

R

Y

Y

A

A

V

L

V

V

W

W

K

K

S

P

A

P

P

H

D

G

L

F

F

F

S

A

R

R

A

F

P

P

D

N

L

L

K

K

V

L

V

V

F

I

S

N

G

L

G

G

A
|
A

G

G

V

V

D

D

H

A

V

L

L

V

K

A

L

R

P

D

G

D

L

L

P

P

D

D

V

V

P

P

L

V

V

T

R
|
R

F

L

V

S

D

D

R

P

T

N

L
x
M

T

S

T

Q

R

M

M
|
M

S

A

E

S

W

F

V

V

V

L

M

F

Q

C

C

V

L

L

L

R

H

H

L

A

R

R

Q

D

H

I

R

P

A

T

Y

F

E

E

A

R

L

A

V

Q

K

R

K

E

H

G

E

R

W

W

R

H

D

Y

L

V

S

H

Q

P

P

R

E

T

A

A

D

E

I

I

T

R

V

V

G

G

I

V

M

L

G

G

M
x
L

G
|
G

V
x
D

L
|
L

G

G

Q

A

D

A

A

A

R

L

K

E

L

L

A

A

V

R

M

H

G

G

F

F

K

D

V

V

I

R

G

G

W
|
W

S
|
S

R
|
R

S
x
T

K

P

R
x
K

A

A

V

L

E

E

G

G

I

V

E

S

T

C

F

F

D

H

A

G

-

L

A

E

G

A

L

L

D

P

A

G

F

F

L

L

A

A

R

G

T

S

D

E

F

I

L

V

V

V

G

V

L

M

L
|
L

P
|
P

L

L

T

T

A

P

D

E

T
|
T

K

R

G

G

I

L

F

M

N

N

A

A

T

E

L

R

F

L

K

A

K

H

L

L

S

P

R

R

K

G

G

A

P

K

F

-

G

-

A

-

P

-

V

-

F

F

I

I

N

N

A
x
V

G

A

R
|
R

G

G

G

P

S

V

Q

V

V

D

E

E

A

A

D

A

I

L

L

I

E

A

C

A

L

L

D

R

S

S

G

G

V

H

L

I

G

A

G

E

A

A

S

T

L

L

D
|
D

V

V

F

F

E

E

K

V

E
|
E

P

P

L

L

S

P

R

V

E

G

S

S

R

P

F

L

W

W

D

A

I

M

P

D

N

N

V

V

Y

L

V

V

T

T

P

P

H
|
H

V

L

A
|
A

A
x
S

S

I

S

A

D

I

V

P

K

R

A

T

L

A

F

A

A

P

H

Q

V

I

E

V

E

E

Q

N

I

I

E

R

R

R

F

I

E

E

S

A

G

G

L

E

A

P

L

V

E

L

H

N

V

Q

V

V

D

D

K

P

V

R

A

R

G

G

Y
|
Y

active site: M98 (≠ L98), R230 (= R234), D254 (= D258), E259 (= E263), H278 (= H282)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A74 (= A74), R92 (= R92), M102 (= M102), L147 (≠ M147), G148 (= G148), D149 (≠ V149), L150 (= L150), W168 (= W168), S169 (= S169), R170 (= R170), T171 (≠ S171), K173 (≠ R173), L201 (= L200), P202 (= P201), T207 (= T206), V228 (≠ A232), R230 (= R234), H278 (= H282), A280 (= A284), S281 (≠ A285), Y315 (= Y319)

3kboA 2.14 angstrom crystal structure of putative oxidoreductase (ycdw) from salmonella typhimurium in complex with NADP
39% identity, 87% coverage: 43:319/319 of query aligns to 35:312/312 of 3kboA

query
sites
3kboA

D

D

L
x
N

S

N

G

P

I

A

D

D

Y

Y

A

A

V

L

V

V

W

W

K

Q

S

P

A

P

P

V

D

E

L

M

F

L

S
x
A

R

-

A

G

P
x
R

D
x
R

L

L

K

K

V

A

V

V

F

F

S

V

G

L

G

G

A

A

G

G

V

V

D

D

H

A

V

I

L

L

-

S

K

K

L

L

P

N

G

A

L

H

P

P

D

E

-

M

-

L

-

D

-

A

-

S

V

I

P

P

L

L

V

F

R
|
R

F

L

V

E

D

D

R

T

T

G

L
x
M

T

G

T

L

R

Q

M
|
M

S

Q

E

E

W

Y

V

A

V

V

M

S

Q

Q

C

V

L

L

L

H

H

W

L

F

R

R

Q

R

H

F

R

D

A

D

Y

Y

E

Q

A

A

L

L

V

K

K

N

K

Q

H

A

E

L

W

W

R

K

D

P

L

L

S

P

Q

E

P

Y

E

T

A

R

A

E

D

E

I

F

T

S

V

V

G

G

I

I

M

M

G
|
G

M
x
A

G
|
G

V
|
V

L
|
L

G

G

Q

A

D

K

A

V

A

A

R

E

K

S

L

L

A

Q

V

A

M

W

G

G

F

F

K

P

V

L

I

R

G

C

W
|
W

S
|
S

R
|
R

S
|
S

K

R

R
x
K

A

S

V

W

E

P

G

G

I

V

E

E

T

S

F

Y

-

V

D

G

A

R

A

E

G

E

L

L

D

R

A

A

F

F

L

L

A

N

R

Q

T

T

D

R

F

V

L

L

V

I

G

N

L
|
L

L

L

P
|
P

L

N

T

T

A

A

D

Q

T

T

K

V

G

G

I

I

F

I

N

N

A

S

T

E

L

L

F

L

K

D

K

Q

L

L

S

-

R

-

K

-

G

-

P

P

F

D

G

G

A

A

P

Y

V

V

F

-

I

L

N

N

A
x
L

G
x
A

R
|
R

G

G

G

V

S

H

Q

V

V

Q

E

E

A

A

D

D

I

L

L

L

E

A

C

A

L

L

D

D

S

S

G

G

V

K

L

L

G

K

G

G

A

A

S

M

L

L

D
|
D

V

V

F

F

E

S

K

Q

E
|
E

P

P

L

L

S

P

R

Q

E

E

S

S

R

P

F

L

W

W

D

R

I

H

P

P

N

R

V

V

Y

A

V

M

T

T

P

P

H
|
H

V

I

A

A

A
|
A

S

V

S

T

D

R

V

P

K

A

A

E

L

A

F

I

A

D

H

Y

V

I

E

S

E

R

Q

T

I

I

E

T

R

Q

F

L

E

E

S

K

G

G

L

E

A

P

L

V

E

T

H

G

V

Q

V

V

D

D

K

R

V

A

A

R

G

G

Y
|
Y

active site: M95 (≠ L98), R227 (= R234), E256 (= E263), H275 (= H282)
binding N-(2-hydroxyethyl)-N,N-dimethyl-3-sulfopropan-1-aminium: N36 (≠ L44), A53 (≠ S61), R55 (≠ P64), R56 (≠ D65)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R89 (= R92), M99 (= M102), G143 (= G146), A144 (≠ M147), G145 (= G148), V146 (= V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), S168 (= S171), K170 (≠ R173), L197 (= L199), P199 (= P201), L225 (≠ A232), A226 (≠ G233), R227 (= R234), D251 (= D258), H275 (= H282), A278 (= A285), Y312 (= Y319)

7jqiA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with alpha-ketoglutarate and NADP+
38% identity, 88% coverage: 40:319/319 of query aligns to 32:312/312 of 7jqiA

query
sites
7jqiA

K

K

G

S

R

G

D

D

L

N

S

D

G

S

I

A

D

D

Y

Y

A

A

V

L

V

V

W

W

K

H

S

P

A

P

P

V

D

E

L

M

F

L

S

A

R

-

A

G

P

R

D

D

L

L

K

K

V

A

V

V

F

F

S

A

G

L

G
|
G

A
|
A

G
|
G

V

V

D

D

H

S

V

I

L

L

-

S

K

K

L

L

P

Q

G

A

L

H

P

P

D

E

-

M

-

L

-

N

-

P

-

S

V

V

P

P

L

L

V

F

R
|
R

F

L

V

E

D

D

R

T

T

G

L

M

T

G

T

E

R

Q

M
|
M

S

Q

E

E

W

Y

V

A

V

V

M

S

Q

Q

C

V

L

L

L

H

H

W

L

F

R

R

Q

R

H

F

R

D

A

D

Y

Y

E

R

A

I

L

Q

V

Q

K

N

K

S

H

S

E

H

W

W

R

Q

D

P

L

L

S

P

Q

E

P

Y

E

H

A

R

A

E

D

D

I

F

T

T

V

I

G

G

I

I

M

L

G

G

M
x
A

G
|
G

V
|
V

L
|
L

G

G

Q

S

D

K

A

V

A

A

R

Q

K

S

L

L

A

Q

V

T

M

W

G

R

F

F

K

P

V

L

I

R

G

C

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R
x
K

A

S

V

W

E

P

G

G

I

V

E

Q

T

S

F

F

D

A

A

G

-

R

A

E

G

E

L

L

D

S

A

A

F

F

L

L

A

S

R

Q

T

C

D

R

F

V

L

L

V

I

G

N

L

L

L
|
L

P
|
P

L

N

T

T

A

P

D

E

T

T

K

V

G

G

I

I

F

I

N

N

A

Q

T

Q

L

L

F

L

K

E

K

K

L

L

S

-

R

-

K

-

G

-

P

P

F

D

G

G

A

A

P

-

V

Y

F

L

I

L

N

N

A
x
L

G

A

R
|
R

G

G

G

V

S

H

Q

V

V

V

E

E

A

D

D

D

I

L

L

L

E

A

C

A

L

L

D

D

S

S

G

G

V

K

L

V

G

K

G

G

A

A

S

M

L

L

D

D

V

V

F

F

E

N

K

R

E

E

P

P

L

L

S

P

R

P

E

E

S

S

R

P

F

L

W

W

D

Q

I

H

P

P

N

R

V

V

Y

T

V

I

T

T

P

P

H
|
H

V

V

A

A

A
|
A

S

I

S

T

D

R

V

P

K

A

A

E

L

A

F

V

A

E

H

Y

V

I

E

S

E

R

Q

T

I

I

E

A

R

Q

F

L

E

E

S

K

G

G

L

E

A

K

L

V

E

C

H

G

V

Q

V

V

D

D

K

R

V

A

A

R

G

G

Y
|
Y

binding 2-oxoglutaric acid: G64 (= G73), A65 (= A74), G66 (= G75), R227 (= R234), H275 (= H282)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A65 (= A74), R89 (= R92), M99 (= M102), A144 (≠ M147), G145 (= G148), V146 (= V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), T168 (≠ S171), K170 (≠ R173), L198 (= L200), P199 (= P201), L225 (≠ A232), R227 (= R234), H275 (= H282), A278 (= A285), Y312 (= Y319)

6p35A 2.5 angstrom structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with 2-keto arginine and NADP
38% identity, 88% coverage: 40:319/319 of query aligns to 32:312/312 of 6p35A

query
sites
6p35A

K

K

G

S

R

G

D

D

L

N

S

D

G

S

I

A

D

D

Y

Y

A

A

V

L

V

V

W

W

K

H

S

P

A

P

P

V

D

E

L

M

F

L

S

A

R

-

A

G

P

R

D

D

L

L

K

K

V

A

V

V

F

F

S

A

G

L

G

G

A
|
A

G

G

V

V

D

D

H

S

V

I

L

L

-

S

K

K

L

L

P

Q

G

A

L

H

P

P

D

E

-

M

-

L

-

N

-

P

-

S

V

V

P

P

L

L

V

F

R
|
R

F

L

V

E

D

D

R

T

T

G

L

M

T

G

T

E

R

Q

M
|
M

S

Q

E

E

W

Y

V

A

V

V

M

S

Q

Q

C

V

L

L

L

H

H

W

L

F

R

R

Q

R

H

F

R

D

A

D

Y

Y

E

R

A

I

L

Q

V

Q

K

N

K

S

H

S

E

H

W

W

R

Q

D

P

L

L

S

P

Q

E

P

Y

E

H

A

R

A

E

D

D

I

F

T

T

V

I

G

G

I

I

M

L

G

G

M
x
A

G
|
G

V
|
V

L
|
L

G

G

Q

S

D

K

A

V

A

A

R

Q

K

S

L

L

A

Q

V

T

M

W

G

R

F

F

K

P

V

L

I

R

G

C

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R
x
K

A

S

V

W

E

P

G

G

I

V

E

Q

T

S

F

F

D

A

A

G

-

R

A

E

G

E

L

L

D

S

A

A

F

F

L

L

A

S

R

Q

T

C

D

R

F

V

L

L

V

I

G

N

L

L

L
|
L

P
|
P

L

N

T

T

A

P

D

E

T

T

K

V

G

G

I

I

F

I

N

N

A

Q

T

Q

L

L

F

L

K

E

K

K

L

L

S

-

R

-

K

-

G

-

P

P

F

D

G

G

A

A

P

-

V

Y

F

L

I

L

N

N

A
x
L

G

A

R
|
R

G

G

G

V

S

H

Q

V

V

V

E

E

A

D

D

D

I

L

L

L

E

A

C

A

L

L

D

D

S

S

G

G

V

K

L

V

G

K

G

G

A

A

S

M

L

L

D

D

V

V

F

F

E

N

K

R

E

E

P

P

L

L

S

P

R

P

E

E

S

S

R

P

F

L

W

W

D

Q

I

H

P

P

N

R

V

V

Y

T

V

I

T

T

P

P

H
|
H

V

V

A
|
A

S

I

S
x
T

D

R

V

P

K

A

A

E

L

A

F

V

A

E

H

Y

V

I

E

S

E

R

Q

T

I

I

E

A

R

Q

F

L

E

E

S

K

G

G

L

E

A

K

L

V

E

C

H

G

V

Q

V

V

D

D

K

R

V

A

A

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: A65 (= A74), R89 (= R92), M99 (= M102), A144 (≠ M147), G145 (= G148), V146 (= V149), L147 (= L150), W165 (= W168), S166 (= S169), R167 (= R170), T168 (≠ S171), K170 (≠ R173), L198 (= L200), P199 (= P201), L225 (≠ A232), R227 (= R234), H275 (= H282), A277 (= A284), A278 (= A285), Y312 (= Y319)
binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: A65 (= A74), R227 (= R234), H275 (= H282), T280 (≠ S287)

Sites not aligning to the query:

binding 5-[(diaminomethylidene)amino]-2-oxopentanoic acid: 10, 11, 15

7jqhA Structure of wild type glyoxylate/hydroxypyruvate reductase a from escherichia coli in complex with phosphate and NADP+
38% identity, 88% coverage: 40:319/319 of query aligns to 33:313/313 of 7jqhA

query
sites
7jqhA

K

K

G

S

R

G

D

D

L

N

S

D

G

S

I

A

D

D

Y

Y

A

A

V

L

V

V

W

W

K

H

S

P

A

P

P

V

D

E

L

M

F

L

S

A

R

-

A

G

P

R

D

D

L

L

K

K

V

A

V

V

F

F

S

A

G

L

G

G

A
|
A

G

G

V

V

D

D

H

S

V

I

L

L

-

S

K

K

L

L

P

Q

G

A

L

H

P

P

D

E

-

M

-

L

-

N

-

P

-

S

V

V

P

P

L

L

V

F

R
|
R

F

L

V

E

D

D

R

T

T

G

L

M

T

G

T

E

R

Q

M
|
M

S

Q

E

E

W

Y

V

A

V

V

M

S

Q

Q

C

V

L

L

L

H

H

W

L

F

R

R

Q

R

H

F

R

D

A

D

Y

Y

E

R

A

I

L

Q

V

Q

K

N

K

S

H

S

E

H

W

W

R

Q

D

P

L

L

S

P

Q

E

P

Y

E

H

A

R

A

E

D

D

I

F

T

T

V

I

G

G

I

I

M

L

G

G

M
x
A

G
|
G

V
|
V

L
|
L

G

G

Q

S

D

K

A

V

A

A

R

Q

K

S

L

L

A

Q

V

T

M

W

G

R

F

F

K

P

V

L

I

R

G

C

W
|
W

S
|
S

R
|
R

S
x
T

K

R

R
x
K

A

S

V

W

E

P

G

G

I

V

E

Q

T

S

F

F

D

A

A

G

-

R

A

E

G

E

L

L

D

S

A

A

F

F

L

L

A

S

R

Q

T

C

D

R

F

V

L

L

V

I

G

N

L

L

L
|
L

P
|
P

L

N

T

T

A

P

D

E

T

T

K

V

G

G

I

I

F

I

N

N

A

Q

T

Q

L

L

F

L

K

E

K

K

L

L

S

-

R

-

K

-

G

-

P

P

F

D

G

G

A

A

P

-

V

Y

F

L

I

L

N

N

A
x
L

G
x
A

R
|
R

G

G

G

V

S

H

Q

V

V

V

E

E

A

D

D

D

I

L

L

L

E

A

C

A

L

L

D

D

S

S

G

G

V

K

L

V

G

K

G

G

A

A

S

M

L

L

D
|
D

V

V

F

F

E

N

K

R

E

E

P

P

L

L

S

P

R

P

E

E

S

S

R

P

F

L

W

W

D

Q

I

H

P

P

N

R

V

V

Y

T

V

I

T

T

P

P

H
|
H

V

V

A

A

A
|
A

S

I

S

T

D

R

V

P

K

A

A

E

L

A

F

V

A

E

H

Y

V

I

E

S

E

R

Q

T

I

I

E

A

R

Q

F

L

E

E

S

K

G

G

L

E

A

K

L

V

E

C

H

G

V

Q

V

V

D

D

K

R

V

A

A

R

G

G

Y
|
Y

binding nadp nicotinamide-adenine-dinucleotide phosphate: A66 (= A74), R90 (= R92), M100 (= M102), A145 (≠ M147), G146 (= G148), V147 (= V149), L148 (= L150), W166 (= W168), S167 (= S169), R168 (= R170), T169 (≠ S171), K171 (≠ R173), L199 (= L200), P200 (= P201), L226 (≠ A232), A227 (≠ G233), R228 (= R234), D252 (= D258), H276 (= H282), A279 (= A285), Y313 (= Y319)

4zqbB Crystal structure of NADP-dependent dehydrogenase from rhodobactersphaeroides in complex with NADP and sulfate
35% identity, 86% coverage: 47:319/319 of query aligns to 48:316/316 of 4zqbB

query
sites
4zqbB

I

V

D

D

Y

I

A

A

V

I

V

V

W

A

K

N

S

P

A

D

P

P

D

A

L

D

F

L

S

A

R

E

A

L

P

P

D

N

L

L

K

V

V

W

V

I

F

H

S

S

G

L

G

W

A

A

G

G

V

V

D

E

H

R

V

L

L

V

K

A

L

E

P

L

G

G

L

H

P

L

D

A

V

R

P

P

L

I

V

V

R
|
R

F

L

V

V

D

D

R

P

T

E

L
|
L

T

A

T

R

R

T

M
|
M

S

A

E

E

W

A

V

A

V

L

M

A

Q

W

C

T

L

Y

H

L

L

F

R

R

Q

D

H

M

R

P

A

A

Y

Y

E

A

A

A

L

Q

V

Q

K

R

K

A

H

R

E

V

W

W

R

K

D

G

L

L

S

P

Q

Y

P

K

E

R

A

P

A

E

D

R

I

T

T

T

V

V

G

G

I

V

M

L

G
|
G

M
x
L

G
|
G

V
x
E

L
|
L

G

G

Q

A

D

A

A

A

R

L

K

R

L

L

A

R

V

D

M

A

G

G

F

F

K

D

V

V

I

H

G

G

W
|
W

S
|
S

R
|
R

S
|
S

K

P

R
x
K

A

E

V

I

E

A

G

G

I

V

E

T

T

C

F

H

D

A

A

G

-

E

A

E

G

T

L

L

D

E

A

R

F

M

L

L

A

G

R

Q

T

V

D

E

F

I

L

L

V

V

G

C

L

L

L
|
L

P
|
P

L

L

T

T

A

G

D

E

T

T

K

R

G

G

I

L

F

L

N

D

A

A

T

R

L

R

F

L

K

A

K

C

L

L

S

-

R

-

K

-

G

-

P

P

F

E

G

G

A

A

P

Q

V

I

F

-

I

V

N

N

A
x
F

G

A

R
|
R

G

G

G

P

S

I

Q

L

V

D

E

S

A

A

D

A

I

L

L

I

E

E

C

A

L

L

D

D

S

S

G

G

V

R

L

I

G

G

G

H

A

A

S

V

L

L

D

D

V

V

F

F

E

E

K

V

E
|
E

P

P

L

L

S

P

R

E

E

A

S

S

R

P

F

F

W

W

D

G

I

H

P

P

N

K

V

V

Y

T

V

V

T

L

P

P

H
|
H

V

I

A
x
S

A
|
A

S

A

S

T

D

D

V

P

K

E

A

T

L

A

F

S

A

A

H

I

V

V

E

G

E

A

Q

H

I

V

E

A

R

D

F

Y

E

R

S

A

G

T

L

G

A

R

L

I

E

P

H

P

V

S

V

V

D

D

K

L

V

T

A

R

G

G

Y
|
Y

active site: L99 (= L98), R231 (= R234), E260 (= E263), H279 (= H282)
binding nadp nicotinamide-adenine-dinucleotide phosphate: R93 (= R92), M103 (= M102), G147 (= G146), L148 (≠ M147), G149 (= G148), E150 (≠ V149), L151 (= L150), W169 (= W168), S170 (= S169), R171 (= R170), S172 (= S171), K174 (≠ R173), L202 (= L200), P203 (= P201), F229 (≠ A232), R231 (= R234), H279 (= H282), S281 (≠ A284), A282 (= A285), Y316 (= Y319)

5mhaB D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
33% identity, 67% coverage: 100:313/319 of query aligns to 93:307/308 of 5mhaB

query
sites
5mhaB

T

T

R

T

M

V

S

G

E

E

W

T

V

V

V

A

M

G

Q

Y

C

M

L

L

L

T

H

F

L

A

R

R

Q

R

H

L

R

H

A

A

Y

Y

E

R

A

D

L

A

V

Q

K

H

K

D

H

H

E

A

W

W

R

-

D

D

L

L

S

P

Q

R

P

Y

E

E

A

E

-

P

-

F

-

T

-

L

A

A

D

G

I

E

T

R

V

V

G

C

I

V

M

V

G

G

M
x
L

G
|
G

V
x
T

L
|
L

G

G

Q

R

D

G

A

V

R

D

K

R

L

A

A

A

V

A

M

L

G

G

F

M

K

E

V

V

I

V

G

G

W

V

S
x
R

R
|
R

S
|
S

K

G

R

D

A

P

V

V

E

D

G

N

I

V

E

S

T

T

-

V

F

Y

D

T

A
x
P

A

D

G

R

L

L

D

H

A

E

F

A

L

I

A

A

R

D

T

A

D

R

F

F

L

V

V

V

G

L

L

A

L
x
T

P
|
P

L

L

T

T

A

D

D

E

T
|
T

K

E

G

G

I

M

F

V

N

A

A

A

T

P

L

E

F
|
F

K
x
E

K

T

L
x
M

S

R

R

E

K

D

G

A

P

S

F

L

G

-

A

-

P

-

V

-

F

-

I

V

N

N

A
x
V

G
x
A

R
|
R

G

G

G

P

S

V

Q

V

V

V

E

E

A

S

D

D

I

L

L

V

E

A

C

A

L

L

D

D

S

S

G

G

V
x
D

L

I

G

A

G

G

A

A

S

A

L

L

D

D

V

V

F

F

E

S

K

E

E

E

P

P

L

L

S

P

R

E

E

D

S

S

R

P

F

L

W

W

D

D

I

F

P

E

N

D

V

V

Y

L

V

I

T

T

P

P

H
|
H

V

V

A
x
S

A

A

S

A

S

T

-

S

-

K

-
x
Y

-

H

-

E

D

D

V

V

K

A

A

A

L

L

F

-

A

-

H

-

V

I

E

R

E

E

Q

N

I

I

E

E

R

K

F

I

E

A

S

T

G

G

L

D

A

E

L

L

E

T

H

N

V

R

V

V

binding 2-Ketohexanoic acid: R226 (= R234), H274 (= H282), Y282 (vs. gap)
binding (2R)-2-hydroxyhexanoic acid: Y282 (vs. gap)
binding magnesium ion: F212 (= F215), E213 (≠ K216), M215 (≠ L218), D243 (≠ V251)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L143 (≠ M147), G144 (= G148), T145 (≠ V149), L146 (= L150), R165 (≠ S169), R166 (= R170), S167 (= S171), P180 (≠ A183), T197 (≠ L200), P198 (= P201), T203 (= T206), V224 (≠ A232), A225 (≠ G233), R226 (= R234), H274 (= H282), S276 (≠ A284)

Sites not aligning to the query:

5mhaA D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with a mixture of 2-ketohexanoic acid and 2-hydroxyhexanoic acid, and NADPH (1.57 a resolution)
33% identity, 67% coverage: 100:313/319 of query aligns to 93:307/308 of 5mhaA

query
sites
5mhaA

T

T

R

T

M

V

S

G

E

E

W

T

V

V

V

A

M

G

Q

Y

C

M

L

L

L

T

H

F

L

A

R

R

Q

R

H

L

R

H

A

A

Y

Y

E

R

A

D

L

A

V

Q

K

H

K

D

H

H

E

A

W

W

R

-

D

D

L

L

S

P

Q

R

P

Y

E

E

A

E

-

P

-

F

-
x
T

-

L

A
|
A

D

G

I

E

T

R

V

V

G

C

I

V

M

V

G

G

M
x
L

G
|
G

V
x
T

L
|
L

G

G

Q

R

D

G

A

V

R

D

K

R

L

A

A

A

V

A

M

L

G

G

F

M

K

E

V

V

I

V

G

G

W

V

S
x
R

R
|
R

S
|
S

K

G

R

D

A

P

V

V

E

D

G

N

I

V

E

S

T

T

-

V

F

Y

D

T

A
x
P

A

D

G

R

L

L

D

H

A

E

F

A

L

I

A

A

R

D

T

A

D

R

F

F

L

V

V

V

G

L

L

A

L
x
T

P
|
P

L

L

T

T

A

D

D

E

T
|
T

K

E

G

G

I

M

F

V

N

A

A

A

T

P

L

E

F

F

K

E

K

T

L

M

S

R

R

E

K

D

G

A

P

S

F

L

G

-

A

-

P

-

V

-

F

-

I

V

N

N

A
x
V

G
x
A

R
|
R

G

G

G

P

S

V

Q

V

V

V

E

E

A

S

D

D

I

L

L

V

E

A

C

A

L

L

D

D

S

S

G

G

V

D

L

I

G

A

G

G

A

A

S

A

L

L

D

D

V

V

F

F

E

S

K

E

E

E

P

P

L

L

S

P

R

E

E

D

S

S

R

P

F

L

W

W

D

D

I

F

P

E

N

D

V

V

Y

L

V

I

T

T

P

P

H
|
H

V

V

A
x
S

A

A

S

A

S

T

-

S

-

K

-
x
Y

-

H

-

E

D

D

V

V

K

A

A

A

L

L

F

-

A

-

H

-

V

I

E

R

E

E

Q

N

I

I

E

E

R

K

F

I

E

A

S

T

G

G

L

D

A

E

L

L

E

T

H

N

V

R

V

V

binding 2-Ketohexanoic acid: R226 (= R234), H274 (= H282), Y282 (vs. gap)
binding magnesium ion: T132 (vs. gap), A134 (= A138)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: L143 (≠ M147), G144 (= G148), T145 (≠ V149), L146 (= L150), R165 (≠ S169), R166 (= R170), S167 (= S171), P180 (≠ A183), T197 (≠ L200), P198 (= P201), T203 (= T206), V224 (≠ A232), A225 (≠ G233), R226 (= R234), H274 (= H282), S276 (≠ A284)

Sites not aligning to the query:

5mh5A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-keto-hexanoic acid and NADP+ (1.4 a resolution)
33% identity, 67% coverage: 100:313/319 of query aligns to 93:307/308 of 5mh5A

query
sites
5mh5A

T

T

R

T

M

V

S

G

E

E

W

T

V

V

V

A

M

G

Q

Y

C

M

L

L

L

T

H

F

L

A

R

R

Q

R

H

L

R

H

A

A

Y

Y

E

R

A

D

L

A

V

Q

K

H

K

D

H

H

E

A

W

W

R

-

D

D

L

L

S

P

Q

R

P

Y

E

E

A

E

-

P

-

F

-
x
T

-

L

A
|
A

D

G

I

E

T

R

V

V

G

C

I

V

M

V

G
|
G

M
x
L

G
|
G

V
x
T

L
|
L

G

G

Q

R

D

G

A

V

R

D

K

R

L

A

A

A

V

A

M

L

G

G

F

M

K

E

V

V

I

V

G

G

W

V

S
x
R

R
|
R

S
|
S

K

G

R

D

A

P

V

V

E

D

G

N

I

V

E

S

T

T

-

V

F

Y

D

T

A

P

A

D

G

R

L

L

D

H

A

E

F

A

L

I

A

A

R

D

T

A

D

R

F

F

L

V

V

V

G

L

L

A

L
x
T

P
|
P

L

L

T

T

A

D

D

E

T
|
T

K

E

G

G

I

M

F

V

N

A

A

A

T

P

L

E

F
|
F

K
x
E

K

T

L
x
M

S

R

R

E

K

D

G

A

P

S

F

L

G

-

A

-

P

-

V

-

F

-

I

V

N

N

A
x
V

G
x
A

R
|
R

G

G

G

P

S

V

Q

V

V

V

E

E

A

S

D

D

I

L

L

V

E

A

C

A

L

L

D

D

S

S

G

G

V
x
D

L

I

G

A

G

G

A

A

S

A

L

L

D

D

V

V

F

F

E

S

K

E

E

E

P

P

L

L

S

P

R

E

E

D

S

S

R

P

F

L

W

W

D

D

I

F

P

E

N

D

V

V

Y

L

V

I

T

T

P

P

H
|
H

V

V

A
x
S

A

A

S

A

S

T

-

S

-

K

-
x
Y

-

H

-

E

D

D

V

V

K

A

A

A

L

L

F

-

A

-

H

-

V

I

E

R

E

E

Q

N

I

I

E

E

R

K

F

I

E

A

S

T

G

G

L

D

A

E

L

L

E

T

H

N

V

R

V

V

binding 2-Ketohexanoic acid: R226 (= R234), H274 (= H282), Y282 (vs. gap)
binding magnesium ion: T132 (vs. gap), A134 (= A138), F212 (= F215), E213 (≠ K216), M215 (≠ L218), D243 (≠ V251)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G142 (= G146), L143 (≠ M147), G144 (= G148), T145 (≠ V149), L146 (= L150), R165 (≠ S169), R166 (= R170), S167 (= S171), T197 (≠ L200), P198 (= P201), T203 (= T206), V224 (≠ A232), A225 (≠ G233), R226 (= R234), H274 (= H282), S276 (≠ A284)

Sites not aligning to the query:

5mh6A D-2-hydroxyacid dehydrogenases (d2-hdh) from haloferax mediterranei in complex with 2-ketohexanoic acid and NAD+ (1.35 a resolution)
33% identity, 67% coverage: 100:313/319 of query aligns to 91:305/306 of 5mh6A

query
sites
5mh6A

T

T

R

T

M

V

S

G

E

E

W

T

V

V

V

A

M

G

Q

Y

C

M

L

L

L

T

H

F

L

A

R

R

Q

R

H

L

R

H

A

A

Y

Y

E

R

A

D

L

A

V

Q

K

H

K

D

H

H

E

A

W

W

R

-

D

D

L

L

S

P

Q

R

P

Y

E

E

A

E

-

P

-

F

-
x
T

-

L

A
|
A

D

G

I

E

T

R

V

V

G

C

I

V

M

V

G

G

M

L

G
|
G

V
x
T

L
|
L

G

G

Q

R

D

G

A

V

R

D

K

R

L

A

A

A

V

A

M

L

G

G

F

M

K

E

V

V

I

V

G

G

W

V

S

R

R
|
R

S

S

K

G

R

D

A

P

V

V

E

D

G

N

I

V

E

S

T

T

-

V

F

Y

D

T

A

P

A

D

G

R

L

L

D

H

A

E

F

A

L

I

A

A

R

D

T

A

D

R

F

F

L

V

V

V

G

L

L

A

L

T

P
|
P

L

L

T

T

A

D

D

E

T
|
T

K

E

G

G

I

M

F

V

N

A

A

A

T

P

L

E

F
|
F

K
x
E

K

T

L
x
M

S

R

R

E

K

D

G

A

P

S

F

L

G

-

A

-

P

-

V

-

F

-

I

V

N

N

A
x
V

G
x
A

R
|
R

G
|
G

G
x
P

S

V

Q
x
V

V

V

E
|
E

A

S

D

D

I

L

L

V

E

A

C

A

L

L

D

D

S

S

G

G

V
x
D

L

I

G

A

G

G

A

A

S

A

L

L

D

D

V

V

F

F

E
x
S

K

E

E

E

P

P

L

L

S

P

R

E

E

D

S

S

R

P

F

L

W

W

D

D

I

F

P

E

N

D

V

V

Y

L

V

I

T

T

P

P

H
|
H

V

V

A
x
S

A

A

S

A

S

T

-

S

-

K

-
x
Y

-

H

-

E

D

D

V

V

K

A

A

A

L

L

F

-

A

-

H

-

V

I

E

R

E

E

Q

N

I

I

E

E

R

K

F

I

E

A

S

T

G

G

L

D

A

E

L

L

E

T

H

N

V

R

V

V

binding 2-Ketohexanoic acid: R224 (= R234), H272 (= H282), Y280 (vs. gap)
binding magnesium ion: T130 (vs. gap), A132 (= A138), F210 (= F215), E211 (≠ K216), M213 (≠ L218), G225 (= G235), P226 (≠ G236), V228 (≠ Q238), E230 (= E240), D241 (≠ V251), S251 (≠ E261)
binding nicotinamide-adenine-dinucleotide: G142 (= G148), T143 (≠ V149), L144 (= L150), R164 (= R170), P196 (= P201), T201 (= T206), V222 (≠ A232), A223 (≠ G233), R224 (= R234), H272 (= H282), S274 (≠ A284)

Sites not aligning to the query:

3dc2A Crystal structure of serine bound d-3-phosphoglycerate dehydrogenase from mycobacterium tuberculosis (see paper)
31% identity, 72% coverage: 58:287/319 of query aligns to 56:282/526 of 3dc2A

query
sites
3dc2A

D

E

L

V

F

L

S

A

R

A

A

A

P

P

D

K

L

L

K

K

V

I

V

V

F

A

S

R

G

A

G

G

A

V

G

G

V

L

D

D

H

N

V

V

L

D

K

V

L

D

P

A

G

A

L

T

P

A

D

R

V

G

P

V

L

L

V

V

R

V

F

N

V

A

D

P

R

T

T

S

L
x
N

T

I

T

H

R

S

M

A

S

A

E

E

W

H

V

A

V

L

M

A

Q

L

C

L

L

L

L

A

H

A

L

S

R

R

Q

Q

H

I

R

P

A

A

Y

A

E

D

A

A

L

S

V

L

K

R

K

E

H

H

E

T

W

W

R

K

-

R

-

S

D

S

L

F

S

S

Q

G

P

T

E

E

A

I

A

F

D

G

I

K

T

T

V

V

G

G

I

V

M

V

G

G

M

L

G

G

V

R

L

I

G

G

Q

Q

D

L

A

V

A

A

R

Q

K

R

L

I

A

A

V

A

M

F

G

G

F

A

K

Y

V

V

I

V

G

A

W

Y

-

D

-

P

-

Y

-

V

S

S

R

P

S

A

K

R

R

A

A

A

V

Q

E

L

G

G

I

I

E

E

T

L

F

L

D

S

A

-

G

-

L

L

D

D

A

D

F

L

L

L

A

A

R

R

T

A

D

D

F

F

L

I

V

S

G

V

L

H

L

L

P

P

L

K

T

T

A

P

D

E

T

T

K

A

G

G

I

L

F

I

N

D

A

K

T

-

L

-

F

-

K

E

K

A

L

L

S

A

R

K

K

T

G

K

P

P

F

-

G

-

A

G

P

V

V

I

F

I

I

V

N

N

A

A

G

A

R
|
R

G

G

S

L

Q

V

V

D

E

E

A

A

D

A

I

L

L

A

E

D

C

A

L

I

D

T

S

G

G

G

V

H

L

V

G

R

G

A

A

A

S

G

L

L

D
|
D

V

V

F

F

E

A

K

T

E
|
E

P

P

L

C

S

T

R

-

E

D

S

S

R

P

F

L

W

F

D

E

I

L

P

A

N

Q

V

V

Y

V

V

V

T

T

P

P

H
|
H

V

L

A

G

A

A

S

S

S

T

active site: N96 (≠ L98), R230 (= R234), D254 (= D258), E259 (= E263), H277 (= H282)

Sites not aligning to the query:

binding serine: 458, 460, 461, 462, 463, 464, 465, 484

3ddnB Crystal structure of hydroxypyruvic acid phosphate bound d-3- phosphoglycerate dehydrogenase in mycobacterium tuberculosis (see paper)
31% identity, 72% coverage: 58:287/319 of query aligns to 57:283/525 of 3ddnB

query
sites
3ddnB

D

E

L

V

F

L

S

A

R

A

A

A

P

P

D

K

L

L

K

K

V

I

V

V

F

A

S
x
R

G

A

G

G

A
x
V

G

G

V

L

D

D

H

N

V

V

L

D

K

V

L

D

P

A

G

A

L

T

P

A

D

R

V

G

P

V

L

L

V

V

R

V

F

N

V

A

D

P

R

T

T

S

L
x
N

T

I

T

H

R

S

M

A

S

A

E

E

W

H

V

A

V

L

M

A

Q

L

C

L

L

L

L

A

H

A

L

S

R

R

Q

Q

H

I

R

P

A

A

Y

A

E

D

A

A

L

S

V

L

K

R

K

E

H

H

E

T

W

W

R

K

-

R

-

S

D

S

L

F

S

S

Q

G

P

T

E

E

A

I

A

F

D

G

I

K

T

T

V

V

G

G

I

V

M

V

G

G

M

L

G

G

V

R

L

I

G

G

Q

Q

D

L

A

V

A

A

R

Q

K

R

L

I

A

A

V

A

M

F

G

G

F

A

K

Y

V

V

I

V

G

A

W

Y

-

D

-

P

-

Y

-

V

S

S

R

P

S

A

K

R

R

A

A

A

V

Q

E

L

G

G

I

I

E

E

T

L

F

L

D

S

A

-

G

-

L

L

D

D

A

D

F

L

L

L

A

A

R

R

T

A

D

D

F

F

L

I

V

S

G

V

L

H

L

L

P

P

L

K

T

T

A

P

D

E

T

T

K

A

G

G

I

L

F

I

N

D

A

K

T

-

L

-

F

-

K

E

K

A

L

L

S

A

R

K

K

T

G

K

P

P

F

-

G

-

A

G

P

V

V

I

F

I

I

V

N

N

A

A

G

A

R
|
R

G

G

S

L

Q

V

V

D

E

E

A

A

D

A

I

L

L

A

E

D

C

A

L

I

D

T

S

G

G

G

V

H

L

V

G

R

G

A

A

A

S

G

L

L

D
|
D

V

V

F

F

E

A

K

T

E
|
E

P

P

L

C

S

T

R

-

E

D

S

S

R

P

F

L

W

F

D

E

I

L

P

A

N

Q

V

V

Y

V

V

V

T

T

P

P

H
|
H

V

L

A

G

A
|
A

S

S

S

T

active site: N97 (≠ L98), R231 (= R234), D255 (= D258), E260 (= E263), H278 (= H282)
binding 2-oxo-3-(phosphonooxy)propanoic acid: R70 (≠ S71), V73 (≠ A74), N97 (≠ L98), A281 (= A285)

Sites not aligning to the query:

binding 2-oxo-3-(phosphonooxy)propanoic acid: 287

5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
31% identity, 86% coverage: 42:315/319 of query aligns to 40:311/318 of 5j23A

query
sites
5j23A

R

R

D

D

L

V

S

S

G

G

I

I

D

-

Y

-

A

A

V

V

V

S

W

G

K

K

S

L

A

P

P

V

D

P

L

L

F

M

S

D

R

A

A

F

P

P

D

S

L

L

K

E

V

I

V

V

F

A

S

N

G

F

G

G

A
x
V

G

G

V

Y

D

D

H

G

V

V

-

D

-

V

-

S

-

R

-

A

-

R

-

G

L

I

K

V

L

V

P

T

G

N

L

T

P

P

D

D

V

V

P

-

L

-

V

-

R

-

F

-

V

-

D

-

R

-

T

-

L
|
L

T

T

T

E

R

E

M

V

S

A

E

D

W

T

V

A

V

I

M

G

Q

L

C

L

L

L

L

N

H

T

L

L

R

R

Q

L

H

L

R

P

A

Q

Y

A

E

E

A

Q

L

W

V

L

K

R

K

Q

H

G

E

R

W

W

-

V

R

R

D

E

-

G

-

A

-

F

-

P

L

L

S

S

Q

P

P

L

E

S

A

L

A

R

D

G

I

R

T

T

V

V

G

G

I

L

M

F

G

G

M
x
L

G
|
G

V
x
R

L
x
I

G

G

Q

L

D

A

A

I

A

A

R

R

K

R

L

L

A

E

V

A

M

F

G

G

F

V

K

S

V

I

I

A

G

Y

W

H

S
x
T

R
|
R

S

T

K

P

R

R

A

-

V

-

E

E

G

G

I

L

E

G

T

F

F

T

D

Y

A

H

A

P

G

T

L

L

D

V

A

G

F

M

L

A

A

E

R

A

T

V

D

D

F

T

L

L

V

I

G

V

L

I

L

V

P
|
P

L

G

T

T

A

A

D
x
S

T
|
T

K

L

G

K

I

A

F

V

N

N

A

A

T

D

L

V

F

L

K

S

K

A

L

L

S

G

R

P

K

K

G

G

P

-

F

-

G

-

A

-

P

-

V

V

F

L

I

I

N

N

A

V

G

G

R
|
R

G

G

G

S

S

T

Q

V

V

D

E

E

A

A

D

A

I

L

L

V

E

T

C

A

L

L

D

Q

S

N

G

G

V

T

L

I

G

A

G

G

A

A

S

G

L

L

D
|
D

V

V

F

F

E

E

K

N

E
|
E

P

P

L

N

S

V

R

P

E

E

S

A

R

-

F

L

W

L

D

S

I

F

P

P

N

N

V

V

Y

S

V

L

T

L

P

P

H
|
H

V

V

A

A

A

S

S

A

S

S

D

V

V

V

-

T

-

R

K

N

A

A

L

M

F

S

A

D

H

L

V

V

E

V

E

D

Q

N

I

L

E

K

R

A

-

W

F

F

E

S

S

T

G

G

L

E

A

A

L

L

E

T

H

P

V

V

V

A

D

E

K

T

active site: L94 (= L98), R228 (= R234), D252 (= D258), E257 (= E263), H275 (= H282)
binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (≠ A74), L148 (≠ M147), G149 (= G148), R150 (≠ V149), I151 (≠ L150), T170 (≠ S169), R171 (= R170), P200 (= P201), S204 (≠ D205), T205 (= T206), R228 (= R234)

5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
31% identity, 86% coverage: 42:315/319 of query aligns to 40:311/317 of 5v7gA

query
sites
5v7gA

R

R

D

D

L

V

S

S

G

G

I

I

D

-

Y

-

A

A

V

V

V

S

W

G

K

K

S

L

A

P

P

V

D

P

L

L

F

M

S

D

R

A

A

F

P

P

D

S

L

L

K

E

V

I

V

V

F

A

S

N

G

F

G
|
G

A
x
V

G
|
G

V

Y

D

D

H

G

V

V

-

D

-

V

-

S

-

R

-

A

-

R

-

G

L

I

K

V

L

V

P

T

G

N

L

T

P

P

D

D

V

V

P

-

L

-

V

-

R

-

F

-

V

-

D

-

R

-

T

-

L
|
L

T

T

T

E

R

E

M
x
V

S

A

E

D

W

T

V

A

V

I

M

G

Q

L

C

L

L

L

L

N

H

T

L

L

R

R

Q

L

H

L

R

P

A

Q

Y

A

E

E

A

Q

L

W

V

L

K

R

K

Q

H

G

E

R

W

W

-

V

R

R

D

E

-

G

-

A

-

F

-

P

L

L

S

S

Q

P

P

L

E

S

A

L

A

R

D

G

I

R

T

T

V

V

G

G

I

L

M
x
F

G

G

M
x
L

G
|
G

V
x
R

L
x
I

G

G

Q

L

D

A

A

I

A

A

R

R

K

R

L

L

A

E

V

A

M

F

G

G

F

V

K

S

V

I

I

A

G

Y

W

H

S
x
T

R
|
R

S

T

K

P

R

R

A

-

V

-

E

E

G

G

I

L

E

G

T

F

F

T

D

Y

A

H

A

P

G

T

L

L

D

V

A

G

F

M

L

A

A

E

R

A

T

V

D

D

F

T

L

L

V

I

G

V

L

I

L
x
V

P
|
P

L

G

T

T

A

A

D
x
S

T
|
T

K

L

G

K

I

A

F

V

N

N

A

A

T

D

L

V

F

L

K

S

K

A

L

L

S

G

R

P

K

K

G

G

P

-

F

-

G

-

A

-

P

-

V

V

F

L

I

I

N

N

A
x
V

G
|
G

R
|
R

G

G

G

S

S

T

Q

V

V

D

E

E

A

A

D

A

I

L

L

V

E

T

C

A

L

L

D

Q

S

N

G

G

V

T

L

I

G

A

G

G

A

A

S

G

L

L

D
|
D

V

V

F

F

E

E

K

N

E
|
E

P

P

L

N

S

V

R

P

E

E

S

A

R

-

F

L

W

L

D

S

I

F

P

P

N

N

V

V

Y

S

V

L

T

L

P

P

H
|
H

V

V

A
|
A

A

S

S

A

S

S

D

V

V

V

-

T

-

R

K

N

A

A

L

M

F

S

A

D

H

L

V

V

E

V

E

D

Q

N

I

L

E

K

R

A

-

W

F

F

E

S

S

T

G

G

L

E

A

A

L

L

E

T

H

P

V

V

V

A

D

E

K

T

active site: L94 (= L98), R228 (= R234), D252 (= D258), E257 (= E263), H275 (= H282)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (≠ A74), V98 (≠ M102), F146 (≠ M145), L148 (≠ M147), G149 (= G148), R150 (≠ V149), I151 (≠ L150), T170 (≠ S169), R171 (= R170), V199 (≠ L200), P200 (= P201), S204 (≠ D205), T205 (= T206), V226 (≠ A232), G227 (= G233), R228 (= R234), H275 (= H282), A277 (= A284)
binding oxalate ion: G69 (= G73), V70 (≠ A74), G71 (= G75), R228 (= R234), H275 (= H282)

5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
31% identity, 86% coverage: 42:315/319 of query aligns to 41:312/319 of 5v7nA

query
sites
5v7nA

R

R

D

D

L

V

S

S

G

G

I

I

D

-

Y

-

A

A

V

V

V

S

W

G

K

K

S

L

A

P

P

V

D

P

L

L

F

M

S

D

R

A

A

F

P

P

D

S

L

L

K

E

V

I

V

V

F

A

S

N

G

F

G
|
G

A
x
V

G
|
G

V

Y

D

D

H

G

V

V

-

D

-

V

-

S

-

R

-

A

-

R

-

G

L

I

K

V

L

V

P

T

G

N

L

T

P

P

D

D

V

V

P

-

L

-

V

-

R

-

F

-

V

-

D

-

R

-

T

-

L
|
L

T

T

T

E

R

E

M
x
V

S

A

E

D

W

T

V

A

V

I

M

G

Q

L

C

L

L

L

L

N

H

T

L

L

R

R

Q

L

H

L

R

P

A

Q

Y

A

E

E

A

Q

L

W

V

L

K

R

K

Q

H

G

E

R

W

W

-

V

R

R

D

E

-

G

-

A

-

F

-

P

L

L

S

S

Q

P

P

L

E

S

A

L

A

R

D

G

I

R

T

T

V

V

G

G

I

L

M

F

G

G

M
x
L

G
|
G

V
x
R

L
x
I

G

G

Q

L

D

A

A

I

A

A

R

R

K

R

L

L

A

E

V

A

M

F

G

G

F

V

K

S

V

I

I

A

G

Y

W

H

S
x
T

R
|
R

S

T

K

P

R

R

A

-

V

-

E

E

G

G

I

L

E

G

T

F

F

T

D

Y

A

H

A

P

G

T

L

L

D

V

A

G

F

M

L

A

A

E

R

A

T

V

D

D

F

T

L

L

V

I

G

V

L

I

L
x
V

P
|
P

L

G

T

T

A

A

D
x
S

T
|
T

K

L

G

K

I

A

F

V

N

N

A

A

T

D

L

V

F

L

K

S

K

A

L

L

S

G

R

P

K

K

G

G

P

-

F

-

G

-

A

-

P

-

V

V

F

L

I

I

N

N

A
x
V

G
|
G

R
|
R

G

G

G

S

S

T

Q

V

V

D

E

E

A

A

D

A

I

L

L

V

E

T

C

A

L

L

D

Q

S

N

G

G

V

T

L

I

G

A

G

G

A

A

S

G

L

L

D
|
D

V

V

F

F

E

E

K

N

E
|
E

P

P

L

N

S

V

R

P

E

E

S

A

R

-

F

L

W

L

D

S

I

F

P

P

N

N

V

V

Y

S

V

L

T

L

P

P

H
|
H

V

V

A
|
A

A
x
S

S

A

S

S

D

V

V

V

-

T

-
x
R

K

N

A

A

L

M

F

S

A

D

H

L

V

V

E

V

E

D

Q

N

I

L

E

K

R

A

-

W

F

F

E

S

S

T

G

G

L

E

A

A

L

L

E

T

H

P

V

V

V

A

D

E

K

T

active site: L95 (= L98), R229 (= R234), D253 (= D258), E258 (= E263), H276 (= H282)
binding 2-keto-D-gluconic acid: G70 (= G73), V71 (≠ A74), G72 (= G75), R229 (= R234), H276 (= H282), S279 (≠ A285), R285 (vs. gap)
binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (≠ A74), V99 (≠ M102), L149 (≠ M147), G150 (= G148), R151 (≠ V149), I152 (≠ L150), T171 (≠ S169), R172 (= R170), V200 (≠ L200), P201 (= P201), S205 (≠ D205), T206 (= T206), V227 (≠ A232), G228 (= G233), R229 (= R234), H276 (= H282), A278 (= A284)

Query Sequence

>WP_012709948.1 NCBI__GCF_000018545.1:WP_012709948.1
MPAKSPVIVDLKFIPEEVDAALNGAFPGREVINLADPVHKGRDLSGIDYAVVWKSAPDLF
SRAPDLKVVFSGGAGVDHVLKLPGLPDVPLVRFVDRTLTTRMSEWVVMQCLLHLRQHRAY
EALVKKHEWRDLSQPEAADITVGIMGMGVLGQDAARKLAVMGFKVIGWSRSKRAVEGIET
FDAAGLDAFLARTDFLVGLLPLTADTKGIFNATLFKKLSRKGPFGAPVFINAGRGGSQVE
ADILECLDSGVLGGASLDVFEKEPLSRESRFWDIPNVYVTPHVAASSDVKALFAHVEEQI
ERFESGLALEHVVDKVAGY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory