SitesBLAST

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
41% identity, 100% coverage: 1:183/183 of query aligns to 2:182/185 of 3nz2J

query
sites
3nz2J

M

M

T

K

R

M

T

S

Q

E

K

L

E

E

K

K

M

M

L

L

A

K

G

G

D

E

M

H

Y

F

N

D

P

G

A

A

D

S

E

A

E

E

I

I

Q

E

T

A

D

L

L

R

L

S

A

Q

T

A

G

G

A

R

W

L

L

K

K

L

R

E

Y

I

N

N

D

Q

T

S

L

L

G

-

S

D

T

E

A

A

E

E

H

R

W

Y

N

-

A

A

L

L

L

Q

L

R

E

E

R

L

L

F

G

G

E

H

V

L

G

G

T

H

G

K

A

S

V

C

I

V

R

Q

P

P

S

P

F

F

H

H

C

C

D

E

Y

F

G

G

F

K

N

T

I

I

R

R

L

I

G

G

A

D

Y

H

V

T

Y

F

I

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

A

A

E

P

V

I

T

T

I

I

G

G

A

D

G

H

T

V

A

L

I

I

G
|
G

P

P

A

S

V

T

Q

Q

I

F

Y
|
Y

T

T

A

A

D

S

H
|
H

P

S

H

L

D

D

P

Y

E

R

Q

R

R

R

Q

Q

A

A

G

W

L

E

Q

T

L

I

G

C

R

K

P

P

V

I

R

V

I

I

G

E

K

D

D

D

V

V

W
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W

I

I

G
|
G

G

G

G

N

A

V

I

V

I

I

L

N

P

Q

G

G

V

V

T

T

I

I

G

G

D

A

N

R

A

S

V

V

I

V

G
x
A

A
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A

G

N

S

S

V

V

T
x
N

R

Q

D

D

I

V

P

P

A

P

G

D

E

T

K

L

A

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M
x
G

G

G

N
x
T

P
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P

A

A

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R

L

I

K

L

R
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R

binding acetyl coenzyme *a: G104 (= G105), Y110 (= Y111), H114 (= H115), W138 (= W139), G140 (= G141), A158 (≠ G159), A159 (= A160), N164 (≠ T165), G174 (≠ M175), T176 (≠ N177), P177 (= P178), R182 (= R183)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
41% identity, 98% coverage: 4:183/183 of query aligns to 2:179/183 of 3nz2C

query
sites
3nz2C

T

S

Q

E

K

L

E

E

K

K

M

M

L

L

A

K

G

G

D

E

M

H

Y

F

N

D

P

G

A

A

D

S

E

A

E

E

I

I

Q

E

T

A

D

L

L

R

L

S

A

Q

T

A

G

G

A

R

W

L

L

K

K

L

R

E

Y

I

N

N

D

Q

T

S

L

L

G

-

S

D

T

E

A

A

E

E

H

R

W

Y

N

-

A

A

L

L

L

Q

L

R

E

E

R

L

L

F

G

G

E

H

V

L

G

G

T

H

G

K

A

S

V

C

I

V

R

Q

P

P

S

P

F

F

H

H

C

C

D

E

Y

F

G

G

F

K

N

T

I

I

R

R

L

I

G

G

A

D

Y

H

V

T

Y

F

I

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

A

A

E

P

V

I

T

T

I

I

G

G

A

D

G

H

T

V

A

L

I

I

G

G

P

P

A

S

V

T

Q

Q

I

F

Y
|
Y

T

T

A

A

D

S

H
|
H

P

S

H

L

D

D

P

Y

E

R

Q

R

R

R

Q

Q

A

A

G

W

L

E

Q

T

L

I

G

C

R

K

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P

V

I

R

V

I

I

G

E

K

D

D

D

V

V

W

W

I

I

G
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G

G

G

G

N

A

V

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V

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I

L

N

P

Q

G

G

V

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T

I

I

G

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D

A

N

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A

S

V

V

I

V

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x
A

A
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A

G
x
N

S

S

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x
N

R

Q

D

D

I

V

P

P

A

P

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K

L

A

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M
x
G

G

G

N
x
T

P
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P

A

A

R

R

L

I

K
x
L

R
|
R

binding acetyl coenzyme *a: Y107 (= Y111), H111 (= H115), G137 (= G141), A155 (≠ G159), A156 (= A160), N161 (≠ T165), G171 (≠ M175), T173 (≠ N177), P174 (= P178), L178 (≠ K182), R179 (= R183)
binding magnesium ion: N157 (≠ G161), T173 (≠ N177)

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
41% identity, 98% coverage: 4:183/183 of query aligns to 2:182/190 of 5u2kA

query
sites
5u2kA

T

T

Q

E

K

K

E

E

K

K

M

M

L

L

A

A

G

E

D

K

M

W

Y

Y

N

-

P

-

A

-

D

D

E

A

E

N

I

F

Q

D

T

Q

D

Y

L

L

L

I

A

N

T

E

G

R

A

A

W

R

L

A

K

K

R

D

Y

I

N

C

D

F

T

E

L

L

G

N

S

H

T

T

-

R

-

P

-

S

A

A

E

T

H

N

W

K

N

R

A

K

L

E

L

L

L

I

E

D

R

Q

L

L

G

F

E

Q

V

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T

T

T

G

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A

N

V

V

-

S

I

I

R

S

P

I

S

P

F

F

H

D

C

T

D

D

Y

Y

G

G

F

W

N

N

I

V

R

K

L

L

G

G

A

K

Y

N

V

V

Y

Y

I

V

N

N

F

T

N

N

C

C

V

Y

I

F

L
x
M

D

D

V

G

A

G

E

Q

V

I

T

T

I

I

G

G

A

D

G

N

T

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A

F

I

I

G

G

P

P

A

N

V

C

Q

G

I

F

Y
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Y

T

T

A
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A

D

T

H
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H

P

P

H

L

D

N

P

F

E

H

Q

H

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N

A

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L

K

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A

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W

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L
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L

P

P

G

G

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V

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T

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I

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E

N

G

A

S

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V

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G

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A

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G

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T

R
x
K

D

D

I

I

P

P

A

P

G

H

E

S

K

L

A

A

M

V

G

G

N

N

P

P

A

C

R

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L

V

K

V

R

R

binding coenzyme a: M90 (≠ L91), Y110 (= Y111), A112 (= A113), H114 (= H115), L146 (= L147), T164 (= T165), K165 (≠ R166)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
42% identity, 97% coverage: 7:183/183 of query aligns to 2:172/176 of 3ectA

query
sites
3ectA

E

E

K

K

M

M

L

L

A

K

G

G

D

H

M

-

Y

-

N

-

P

-

A

A

D

S

E

A

E

E

I

I

Q

E

T

A

D

L

L

R

L

S

A

Q

T

A

G

G

A

R

W

L

L

K

K

L

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Y

I

N

N

D

Q

T

S

L

L

G

-

S

D

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E

A

A

E

E

H

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Y

N

-

A

A

L

L

L

Q

L

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E

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R

L

L

F

G

G

E

H

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L

G

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P

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F

F

H

H

C

C

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Y

F

G

G

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K

N

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I

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R

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I

G

G

A

D

Y

H

V

T

Y

F

I

I

N

N

F

M

N

N

C

V

V

V

I

M

L

L

D

D

V

G

A

A

E

P

V

I

T

T

I

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G

G

A

D

G

H

T

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A

L

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G

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P

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Q

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F

Y

Y

T

T

A

A

D

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H

H

P

S

H

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D

P

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E

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Q

Q

A

A

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L

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C

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K

D

D

D

V

V

W

W

I

I

G

G

G

N

A

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L

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P

Q

G
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G

V

V

T

T

I

I

G

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D

A

N

R

A

S

V

V

I

V

G

A

A

A

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N

S

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D
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D

I

V

P

P

A

P

G

D

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T

K

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G

G

G

N

T

P

P

A

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L

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R

binding calcium ion: G138 (= G149), D156 (= D167)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
38% identity, 100% coverage: 1:183/183 of query aligns to 1:183/203 of P07464

query
sites
P07464

M
|
M

T

N

R

M

T

P

Q

M

K

T

E

E

K

R

M

I

L

R

A

A

G

G

D

K

M

L

Y

F

N

T

P
x
D

A

M

D

C

E

E

E

G

I

L

Q

P

T

E

D

K

L

R

L

L

A

R

T

G

G

K

A

T

W

L

L

M

K

Y

R

E

Y

F

N

N

D

H

T

S

L

H

G

P

S

S

T

E

A

V

E

E

H

K

W

R

N

E

A

S

L

L

L

I

L

K

E

E

R

M

L

F

G

A

E

T

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V

G

G

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G

N

A

A

V

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V

R

E

P

P

S

P

F

V

H

Y

C

F

D
x
S

Y

Y

G

G

F

S

N

N

I

I

R

H

L

I

G

G

A

R

Y

N

V

F

Y

Y

I

A

N
|
N

F

F

N

N

C

L

V

T

I

I

L

V

D

D

V
x
D

A

Y

E

T

V

V

T

T

I

I

G

G

A

D

G

N

T

V

A

L

I

I

G

A

P

P

A

N

V

V

Q

T

I

L

Y

S

T

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A

T

D

G

H
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H

P

P

H

V

D

H

P

H

E

E

Q

L

R

R

Q

K

A

N

G

G

L

E

Q

M

L

Y

G

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P

V

I

R

T

I

I

G

G

K

N

D

N

V

V

W

W

I

I

G

G

G
x
S

G

H

A

V

I

V

I

I

L

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

A

S

V

V

I

I

G

G

A
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A

G

G

S

S

V

I

V

V

T
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T

R
x
K

D

D

I

I

P

P

A

P

G

N

E

V

K

V

A

A

M

A

G

G

N

V

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P

A

C

R
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R

L

V

K

I

R
|
R

M1 (= M1) modified: Initiator methionine, Removed; Partial
D17 (≠ P17) binding substrate
S71 (≠ D71) binding substrate
N85 (= N85) binding in other chain; binding in other chain
D93 (≠ V93) binding substrate
H115 (= H115) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ G142) binding in other chain
A160 (= A160) binding in other chain
TK 165:166 (≠ TR 165:166) binding acetyl-CoA
R180 (= R180) binding acetyl-CoA
R183 (= R183) binding in other chain

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
39% identity, 97% coverage: 7:183/183 of query aligns to 6:182/201 of 1krvA

query
sites
1krvA

E

E

K

R

M

I

L

R

A

A

G

G

D

K

M

L

Y

F

N

T

P
x
D

A

M

D

C

E

E

E

G

I

L

Q

P

T

E

D

K

L
x
R

L

L

A

R

T

G

G

K

A

T

W

L

L

M

K

Y

R

E

Y

F

N

N

D

H

T

S

L

H

G

P

S

S

T

E

A

V

E

E

H

K

W

R

N

E

A

S

L

L

L

I

L

K

E

E

R

M

L

F

G

A

E

T

V

V

G

G

T

E

G

N

A

A

V

W

I

V

R

E

P

P

S

P

F

V

H

Y

C

F

D
x
S

Y

Y

G

G

F

S

N

N

I

I

R

H

L

I

G

G

A

R

Y

N

V

F

Y
|
Y

I

A

N
|
N

F

F

N

N

C

L

V

T

I

I

L
x
V

D

D

V
x
D

A

Y

E

T

V

V

T

T

I

I

G

G

A

D

G

N

T

V

A

L

I

I

G
x
A

P

P

A

N

V

V

Q

T

I

L

Y
x
S

T

V

A
x
T

D

G

H

H

P

P

H

V

D

H

P

H

E

E

Q

L

R

R

Q

K

A

N

G

G

L

E

Q
x
M

L

Y

G

S

R

F

P

P

V

I

R

T

I

I

G

G

K

N

D

N

V

V

W
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W

I

I

G
|
G

G
x
S

G

H

A

V

I

V

I

I

L
x
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

A

S

V

V

I

I

G

G

A
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A

G

G

S

S

V

I

V

V

T

T

R

K

D

D

I

I

P

P

A

P

G

N

E

V

K

V

A

A

M

A

G

G

N

V

P
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P

A

C

R
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R

L

V

K

I

R
|
R

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (≠ P17), R25 (≠ L26), S70 (≠ D71), Y82 (= Y83), N84 (= N85), V90 (≠ L91), D92 (≠ V93), M126 (≠ Q127)
binding coenzyme a: A104 (≠ G105), S110 (≠ Y111), T112 (≠ A113), W138 (= W139), G140 (= G141), S141 (≠ G142), N146 (≠ L147), A159 (= A160), P177 (= P178), R179 (= R180), R182 (= R183)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
39% identity, 97% coverage: 7:183/183 of query aligns to 6:182/201 of 1kruA

query
sites
1kruA

E

E

K

R

M

I

L

R

A

A

G

G

D

K

M

L

Y

F

N

T

P
x
D

A

M

D

C

E

E

E

G

I

L

Q
x
P

T

E

D

K

L
x
R

L

L

A

R

T

G

G

K

A

T

W

L

L

M

K

Y

R

E

Y

F

N

N

D

H

T

S

L

H

G

P

S

S

T

E

A

V

E

E

H

K

W

R

N

E

A

S

L

L

L

I

L

K

E

E

R

M

L

F

G

A

E

T

V

V

G

G

T

E

G

N

A

A

V
x
W

I

V

R

E

P

P

S

P

F

V

H

Y

C

F

D
x
S

Y

Y

G

G

F

S

N

N

I

I

R

H

L

I

G

G

A

R

Y

N

V

F

Y
|
Y

I

A

N
|
N

F

F

N

N

C

L

V

T

I

I

L
x
V

D

D

V
x
D

A

Y

E

T

V

V

T

T

I

I

G

G

A

D

G

N

T

V

A
x
L

I

I

G

A

P

P

A

N

V

V

Q

T

I

L

Y

S

T

V

A

T

D

G

H
|
H

P

P

H

V

D

H

P

H

E

E

Q

L

R

R

Q

K

A

N

G

G

L

E

Q
x
M

L

Y

G

S

R

F

P

P

V

I

R

T

I

I

G

G

K

N

D

N

V

V

W
|
W

I

I

G
|
G

G
x
S

G

H

A

V

I

V

I

I

L

N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

A

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

T

T

R

K

D

D

I

I

P

P

A

P

G

N

E

V

K

V

A

A

M

A

G

G

N

V

P
|
P

A

C

R

R

L

V

K

I

R
|
R

binding coenzyme a: H114 (= H115), W138 (= W139), G140 (= G141), S141 (≠ G142), G158 (= G159), A159 (= A160), P177 (= P178), R182 (= R183)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (≠ P17), P22 (≠ Q23), R25 (≠ L26), W62 (≠ V63), S70 (≠ D71), Y82 (= Y83), Y82 (= Y83), N84 (= N85), V90 (≠ L91), D92 (≠ V93), L102 (≠ A103), H114 (= H115), M126 (≠ Q127)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
39% identity, 97% coverage: 7:183/183 of query aligns to 6:182/200 of 1krrA

query
sites
1krrA

E

E

K

R

M

I

L

R

A

A

G

G

D

K

M

L

Y

F

N

T

P

D

A

M

D

C

E

E

E

G

I

L

Q

P

T

E

D

K

L

R

L

L

A

R

T

G

G

K

A

T

W

L

L

M

K

Y

R

E

Y

F

N

N

D

H

T

S

L

H

G

P

S

S

T

E

A

V

E

E

H

K

W

R

N

E

A

S

L

L

L

I

L

K

E

E

R

M

L

F

G

A

E

T

V

V

G

G

T

E

G

N

A

A

V

W

I

V

R

E

P

P

S

P

F

V

H

Y

C

F

D

S

Y

Y

G

G

F

S

N

N

I

I

R

H

L

I

G

G

A

R

Y

N

V

F

Y

Y

I

A

N
|
N

F

F

N

N

C

L

V

T

I

I

L

V

D

D

V

D

A

Y

E

T

V

V

T

T

I

I

G

G

A

D

G

N

T

V

A

L

I
|
I

G
x
A

P
|
P

A

N

V

V

Q

T

I

L

Y

S

T

V

A
x
T

D

G

H
|
H

P

P

H

V

D

H

P

H

E

E

Q

L

R

R

Q

K

A

N

G

G

L

E

Q

M

L

Y

G

S

R

F

P

P

V

I

R

T

I

I

G

G

K

N

D

N

V

V

W

W

I

I

G
|
G

G
x
S

G

H

A

V

I

V

I

I

L
x
N

P

P

G

G

V

V

T

T

I

I

G

G

D

D

N

N

A

S

V

V

I

I

G
|
G

A
|
A

G

G

S

S

V

I

V

V

T

T

R

K

D

D

I

I

P

P

A

P

G

N

E

V

K

V

A

A

M
x
A

G

G

N

V

P
|
P

A

C

R

R

L

V

K

I

R
|
R

binding acetyl coenzyme *a: N84 (= N85), I103 (= I104), A104 (≠ G105), P105 (= P106), T112 (≠ A113), H114 (= H115), G140 (= G141), S141 (≠ G142), N146 (≠ L147), G158 (= G159), A159 (= A160), A174 (≠ M175), P177 (= P178), R182 (= R183)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
35% identity, 51% coverage: 89:181/183 of query aligns to 183:276/307 of A1ADJ6

query
sites
A1ADJ6

V

V

I

V

L

T

D

D

V

K

A

C

E

N

V

V

T

T

I

I

G

G

A

H

G

D

T

C

A

M

I

I

G

A

P

R

A

D

V

V

Q

I

I

L

Y

R

T

A

A

S

D

D

-

G

H
|
H

P

P

H

I

D

F

P

D

E

I

Q

H

R

S

Q

K

A

K

G

R

L

I

Q

N

L

W

G

A

R

K

P

D

V

I

R

I

I

I

G

S

K

S

D

Y

V

V

W
|
W

I

V

G

G

G

R

G

N

A

V

I

S

I

I

L

M

P

K

G

G

V

V

T

S

I

V

G

G

D

S

N

G

A

S

V

V

I

I

G

G

A

Y

G

G

S

S

V

I

V

V

T

T

R

K

D

D

I

V

P

P

A

S

G

M

E

C

K

A

A

A

M

A

G

G

N

N

P

P

A

A

R

K

L

I

H210 (= H115) mutation to A: Loss of activity.
W234 (= W139) mutation to A: Loss of activity.

8vr6A Crystal structure of the pcryo_0619 n-acetryltransferase from psychrobacter cryohalolentis k5 in the presence of coa-disulfide
32% identity, 84% coverage: 26:179/183 of query aligns to 13:165/177 of 8vr6A

query
sites
8vr6A

L

F

L

L

A

R

T

T

G

T

A

Y

W

Y

L

L

K

K

R

K

Y

Y

N

N

D

R

T

S

L

L

-

P

-

F

-

G

G

D

S

M

T

I

A

V

E

D

H

R

W

W

N

D

A

K

L

A

L

K

L

L

E

L

R

G

L

F

G

G

E

E

V

-

G

G

T

T

G

-

A

S

V

I

I

Y

R

D

P

S

S

S

F

I

H

V

C

L

D

G

Y

-

G

-

F

-

N

E

I

V

R

K

L

V

G

G

A

K

Y

D

V

T

Y

W

I

I

N

G

F

P

N

N

C

-

V

T

I

I

L

L

D

D

V

G

A

S

E

G

V

G

T

G

I

L

G

I

A

I

-

G

-

S

G

N

T

C

A

S

I

I

G

S

P

A

A

G

V

V

Q

Q

I

I

Y
|
Y

T

T

A
x
H

D
|
D

H
x
T

P

V

H

R

D

K

P

S

E

L

Q

S

R

G

Q

G

A

K

G

A

L

D

Q

I

L

D

G

K

R

A

P

S

V

T

R

R

I

I

G

G

K

S

D

D

V

C

W

Y

I

L

G

G

G

P

G

N

A

T

I

I

L
x
V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

N

R

A

V

V

V

I

V

G

G

A

A

G

N

S

S

V
x
L

V

V

T
x
L

R

K

D

D

I

I

P

P

A

S

G

D

E

C

K

K

A

V

M

F

G

G

N
x
S

P
|
P

A

A

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: Y97 (= Y111), H99 (≠ A113), D100 (= D114), T101 (≠ H115), V133 (≠ L147), L149 (≠ V163), L151 (≠ T165), S163 (≠ N177), P164 (= P178)

8vr7B Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
32% identity, 84% coverage: 26:179/183 of query aligns to 14:166/177 of 8vr7B

query
sites
8vr7B

L

F

L

L

A

R

T

T

G

T

A

Y

W

Y

L

L

K

K

R

K

Y

Y

N

N

D

R

T

S

L

L

-

P

-

F

-

G

G

D

S

M

T

I

A

V

E

D

H
x
R

W
|
W

N

D

A

K

L

A

L

K

L

L

E

L

R

G

L

F

G

G

E

E

V

-

G

G

T

T

G

-

A

S

V

I

I

Y

R

D

P

S

S

S

F

I

H

V

C

L

D

G

Y

-

G

-

F

-

N

E

I

V

R

K

L

V

G

G

A

K

Y

D

V

T

Y

W

I

I

N

G

F

P

N

N

C

-

V

T

I

I

L

L

D

D

V

G

A

S

E

G

V

G

T

G

I

L

G
x
I

A

I

-

G

-

S

G

N

T

C

A

S

I

I

G

S

P

A

A

G

V

V

Q

Q

I

I

Y
|
Y

T

T

A

H

D
|
D

H
x
T

P

V

H

R

D

K

P

S

E

L

Q

S

R

G

Q

G

A

K

G

A

L

D

Q

I

L

D

G

K

R

A

P

S

V

T

R
|
R

I

I

G

G

K

S

D

D

V

C

W
x
Y

I

L

G

G

G
x
P

G

N

A

T

I

I

L

V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

N

R

A

V

V

V

I

V

G
|
G

A
|
A

G

N

S

S

V
x
L

V

V

T
x
L

R

K

D

D

I

I

P

P

A

S

G

D

E

C

K
|
K

A

V

M
x
F

G

G

N

S

P
|
P

A

A

binding acetyl coenzyme *a: Y98 (= Y111), D101 (= D114), T102 (≠ H115), L150 (≠ V163), L152 (≠ T165), P165 (= P178)
binding coenzyme a: Y126 (≠ W139), P129 (≠ G142), G146 (= G159), A147 (= A160), K160 (= K173), F162 (≠ M175)
binding 3-[4-(2-hydroxyethyl)piperazin-1-yl]propane-1-sulfonic acid: R37 (≠ H46), W38 (= W47)
binding 3'-phosphate-adenosine-5'-diphosphate: I84 (≠ G99), R120 (= R133)

Sites not aligning to the query:

binding coenzyme a: 175

8vr7E Crystal structure of the pcryo_0619 n-acetyltransferase from psychrobacter cryohalolentis k5 int he presence of acetyl coenzyme a
32% identity, 84% coverage: 26:179/183 of query aligns to 14:166/180 of 8vr7E

query
sites
8vr7E

L

F

L

L

A

R

T

T

G

T

A

Y

W

Y

L

L

K

K

R

K

Y
|
Y

N

N

D
x
R

T

S

L

L

-

P

-

F

-

G

G

D

S

M

T

I

A

V

E

D

H
x
R

W
|
W

N

D

A

K

L

A

L

K

L

L

E

L

R

G

L

F

G

G

E

E

V

-

G

G

T

T

G

-

A

S

V

I

I

Y

R

D

P

S

S

S

F

I

H

V

C

L

D

G

Y

-

G

-

F

-

N

E

I

V

R

K

L

V

G

G

A

K

Y

D

V

T

Y
x
W

I

I

N

G

F

P

N

N

C

-

V

T

I

I

L

L

D

D

V

G

A

S

E

G

V

G

T

G

I

L

G

I

A

I

-

G

-

S

G

N

T

C

A

S

I

I

G
x
S

P

A

A

G

V

V

Q

Q

I

I

Y
|
Y

T

T

A

H

D
|
D

H
x
T

P

V

H

R

D

K

P

S

E

L

Q

S

R

G

Q

G

A

K

G

A

L

D

Q

I

L

D

G

K

R

A

P

S

V

T

R

R

I

I

G

G

K

S

D

D

V

C

W
x
Y

I

L

G

G

G

P

G

N

A

T

I

I

L
x
V

P

K

G

G

V

V

T

K

I

I

G

G

D

D

N

R

A

V

V

V

I

V

G
|
G

A
|
A

G

N

S

S

V
x
L

V

V

T
x
L

R

K

D

D

I

I

P

P

A

S

G

D

E

C

K
|
K

A

V

M
x
F

G

G

N
x
S

P
|
P

A

A

binding acetyl coenzyme *a: W69 (≠ Y83), S92 (≠ G105), Y98 (= Y111), D101 (= D114), T102 (≠ H115), Y126 (≠ W139), V134 (≠ L147), G146 (= G159), A147 (= A160), L150 (≠ V163), L152 (≠ T165), K160 (= K173), F162 (≠ M175), S164 (≠ N177), P165 (= P178)
binding 3'-phosphate-adenosine-5'-diphosphate: Y24 (= Y36), R26 (≠ D38), R37 (≠ H46), W38 (= W47)

Sites not aligning to the query:

binding acetyl coenzyme *a: 175

8j40A Crystal structure of catb8 in complex with chloramphenicol (see paper)
36% identity, 49% coverage: 92:180/183 of query aligns to 52:158/209 of 8j40A

query
sites
8j40A

D

D

V

V

A

D

E

K

V

L

T

I

I

I

G

G

A

S

G

F

T

C

A

S

I

I

G

G

P

S

A

G

V

A

Q

S

I

F

Y

I

T

M

A

A

D

G

-

N

-

Q

-

G

H

H

P

Q

H

H

D

D

-

W

-

A

-

S

-

F

-

P

-

F

-
x
Y

-

M

-

Q

-

E

P

P

E

A

Q

F

R

S

Q

R

A

A

-

L

-

D

G

A

L

F

Q

Q

L

R

G

A

R

G

P

D

V

T

R

V

I

I

G

G

K

N

D

D

V

V

W
|
W

I

I

G

G

G

S

G

E

A

A

I

M

I

I

L

M

P

P

G

G

V

I

T

K

I

I

G

G

D

D

N

G

A

A

V
|
V

I

I

G

G

A
x
S

G

R

S

S

V

L

V

V

T

T

R

K

D

D

I

V

P

E

A

P

G

Y

E

A

K

I

A

I

M

G

G

G

N

N

P

P

A

A

R

K

binding chloramphenicol: Y90 (vs. gap), W117 (= W139), V135 (= V157), S138 (≠ A160)

Sites not aligning to the query:

binding chloramphenicol: 7, 8, 9, 29, 31, 47, 50, 187, 190, 191, 202, 203, 206

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
34% identity, 48% coverage: 95:181/183 of query aligns to 46:139/290 of 4mzuB

query
sites
4mzuB

E

D

V

V

T

V

I

I

G

G

A

D

G

N

T

V

A

T

I

I

G

K

P

S

A
x
G

V

V

Q

Q

I

I

Y

W

T

D

A

G

D

I

H

H

P

I

H

Q

D

D

-

D

-

V

-
x
F

-

I

-

G

P

P

E
x
N

Q

V

R

T

Q

F

A
x
T

G
x
N

L
x
D

Q
x
K

L

Q

G
x
P

R

R

P

S

V

L

R

K

-

T

-

I

I

V

G

K

K

K

D

G

V

A

W

S

I

I

G
|
G

G
x
A

G

N

A

S

I

T

I

I

L

L

P

P

G

G

V

I

T

L

I

I

G

G

D

E

N

N

A

A

V

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

T
|
T

R
x
K

D

N

I

V

P

P

A

D

G

N

E

A

K

I

A

V

M
x
I

G

G

N

N

P

P

A

G

R
|
R

L

I

binding coenzyme a: F72 (vs. gap), T80 (≠ A124), N81 (≠ G125), D82 (≠ L126), K83 (≠ Q127), P85 (≠ G129), G99 (= G141), A100 (≠ G142), G117 (= G159), A118 (= A160), T123 (= T165), K124 (≠ R166), I133 (≠ M175), R138 (= R180)
binding magnesium ion: G58 (≠ A107), N76 (≠ E120)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymine: 20, 21, 38, 39
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
33% identity, 48% coverage: 95:181/183 of query aligns to 46:140/294 of 4mzuF

query
sites
4mzuF

E

D

V

V

T

V

I

I

G

G

A

D

G

N

T

V

A

T

I

I

G

K

P

S

A

G

V

V

Q

Q

I

I

Y

W

T

D

A

G

D

I

H

H

P

I

H

Q

D

D

-

D

-

V

-

F

-

I

-

G

-

P

-

N

-

V

-

T

-

F

P

T

E
x
N

Q
x
D

R
x
K

Q

Q

A

P

G

R

L

S

Q

K

L

I

G

K

R

T

P

I

V

V

R

K

I

K

G

G

K

A

D

S

V

-

W

-

I

I

G
|
G

G
x
A

G

N

A

S

I

T

I

I

L
|
L

P

P

G

G

V

I

T

L

I

I

G

G

D

E

N

N

A

A

V

M

I

V

G
|
G

A
|
A

G

G

S

A

V

V

V

I

T
|
T

R
x
K

D

N

I

V

P

P

A

D

G

N

E

A

K

I

A

V

M
x
I

G

G

N
|
N

P

P

A

G

R
|
R

L

I

binding coenzyme a: N81 (≠ E120), D82 (≠ Q121), K83 (≠ R122), G100 (= G141), A101 (≠ G142), L106 (= L147), G118 (= G159), A119 (= A160), T124 (= T165), K125 (≠ R166), I134 (≠ M175), N136 (= N177), R139 (= R180)

Sites not aligning to the query:

binding thymine: 20, 21, 38
binding thymidine-5'-diphosphate: 189, 203, 205, 270

4eaaA X-ray crystal structure of the h141n mutant of perosamine n- acetyltransferase from caulobacter crescentus in complex with coa and gdp-perosamine (see paper)
36% identity, 66% coverage: 62:182/183 of query aligns to 98:208/210 of 4eaaA

query
sites
4eaaA

A

A

V

V

I

V

R

S

P

P

S

S

F

V

H

R

C

L

D

G

Y

E

G

G

F

V

N

A

I

V

R

M

L

A

G

G

A

-

Y

-

V

V

Y

A

I

I

N

N

F

A

N

D

C

S

V

W

I

I

L

G

D

D

V

L

A

A

E

I

V

I

T

N

I

T

G

G

A

A

G

-

T

-

A

V

I

V

G

D

P

N

A

D

V

C

Q

R

I

L

Y

G

T

A

A

A

D

C

H

H

P

-

H

L

D

G

P
|
P

E

A

Q

S

R

A

Q

L

A

A

G

G

L

-

Q

-

L

-

G

-

R

-

P

G

V

V

R

S

I

V

G

G

K

E

D

R

V

A

W
x
F

I

L

G

G

G
x
V

G

G

A

A

I

R

I

V

L

I

P

P

G

G

V

V

T

T

I

I

G

G

D

A

N

D

A

T

V

I

I

V

G

G

A
|
A

G

G

S

G

V

V

T

V

R

R

D

D

I

L

P

P

A

D

G

S

E

V

K

L

A

A

M
x
I

G

G

N

V

P

P

A

A

R

K

L

I

K
|
K

binding coenzyme a: P150 (= P119), F165 (≠ W139), V168 (≠ G142), A186 (= A160), I201 (≠ M175), K208 (= K182)

Sites not aligning to the query:

binding GDP-perosamine: 14, 15, 16, 35, 36, 37, 52, 70, 71

P50870 Streptogramin A acetyltransferase; Virginiamycin acetyltransferase D; Vat(D); EC 2.3.1.- from Enterococcus faecium (Streptococcus faecium) (see paper)
38% identity, 47% coverage: 95:180/183 of query aligns to 60:162/209 of P50870

query
sites
P50870

E

K

V

L

T

K

I

I

G

G

A

K

G

F

T

C

A

S

I

I

G

G

P

P

A

G

V

V

Q

T

I

I

Y

I

T

M

-

N

-

G

A

A

D

N

H
|
H

P

R

H

M

D

D

P

G

E

S

Q

T

R

Y

Q

P

A

F

G

N

L

L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

-

M

-

P

Q

K

L

L

G

D

R

Q

-

L

P

P

V

I

R

K

-

G

-

D

-

T

-

I

I

I

G

G

K

N

D

D

V

V

W

W

I

I

G

G

G

K

G

D

A

V

I

V

I

I

L

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

N

G

A

A

V

I

I

V

G

A

A

A

G

N

S

S

V

V

T

V

R

K

D

D

I

I

P

A

A

P

G

Y

E

M

K

L

A

A

M

G

G

G

N

N

P

P

A

A

R

N

H82 (= H115) mutation to A: 105-fold decrease in activity.

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
38% identity, 47% coverage: 95:180/183 of query aligns to 60:162/206 of 1khrA

query
sites
1khrA

E

K

V

L

T

K

I

I

G

G

A

K

G

F

T

C

A

S

I

I

G

G

P

P

A

G

V

V

Q

T

I

I

Y

I

T

M

-

N

-

G

A
|
A

D
x
N

H
|
H

P

R

H
x
M

D

D

P

G

E

S

Q

T

R

Y

Q

P

A

F

G

N

L
|
L

-

F

-

G

-

N

-

G

-

W

-

E

-

K

-

H

-

M

-

P

Q

K

L

L

G

D

R

Q

-

L

P

P

V

I

R

K

-

G

-

D

-

T

-

I

I

I

G

G

K

N

D

D

V

V

W
|
W

I

I

G

G

G

K

G

D

A

V

I

V

I

I

L

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

N

G

A

A

V

I

I

V

G
x
A

A
|
A

G

N

S

S

V

V

T
x
V

R

K

D

D

I

I

P

A

A

P

G

Y

E

M

K
x
L

A

A

M
x
G

G

G

N

N

P
|
P

A

A

R

N

binding coenzyme a: H82 (= H115), M84 (≠ H117), W121 (= W139), A141 (≠ G159), A142 (= A160), V147 (≠ T165), L155 (≠ K173), G157 (≠ M175), P160 (= P178)
binding virginiamycin m1: A80 (= A113), N81 (≠ D114), H82 (= H115), L93 (= L126)

Sites not aligning to the query:

Query Sequence

>WP_012755323.1 NCBI__GCF_000023185.1:WP_012755323.1
MTRTQKEKMLAGDMYNPADEEIQTDLLATGAWLKRYNDTLGSTAEHWNALLLERLGEVGT
GAVIRPSFHCDYGFNIRLGAYVYINFNCVILDVAEVTIGAGTAIGPAVQIYTADHPHDPE
QRQAGLQLGRPVRIGKDVWIGGGAIILPGVTIGDNAVIGAGSVVTRDIPAGEKAMGNPAR
LKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory