SitesBLAST
Comparing WP_012756106.1 NCBI__GCF_000023185.1:WP_012756106.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7rbxC Crystal structure of isocitrate lyase and phosphorylmutase:isocitrate lyase from brucella melitensis biovar abortus 2308 bound to itaconic acid (see paper)
88% identity, 99% coverage: 2:426/429 of query aligns to 1:425/425 of 7rbxC
6lrtA Crystal structure of isocitrate lyase (caur_3889) from chloroflexus aurantiacus in complex with isocitrate and manganese ion
75% identity, 96% coverage: 15:424/429 of query aligns to 18:423/423 of 6lrtA
7cmyC Isocitrate lyase from bacillus cereus atcc 14579 in complex with magnessium ion, glyoxylate, and succinate
69% identity, 96% coverage: 12:424/429 of query aligns to 14:417/417 of 7cmyC
7rb1A Isocitrate lyase-1 from mycobacterium tuberculosis covalently modified by 5-descarboxy-5-nitro-d-isocitric acid (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 22:427/427 of 7rb1A
- binding dihydroxyacetic acid: Y89 (= Y82), S91 (= S84), W93 (= W86), D153 (= D147), R228 (= R222), T347 (= T343)
- binding (3E)-3-(hydroxyimino)propanoic acid: C191 (= C185), G192 (= G186), H193 (= H187), R228 (= R222), S315 (= S311), S317 (= S313), T347 (= T343)
- binding magnesium ion: A276 (= A272), A279 (≠ C275), Q308 (≠ K304)
6wsiA Intact cis-2,3-epoxysuccinic acid bound to isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 22:427/427 of 6wsiA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ C275), Q308 (≠ K304)
- binding (2R,3S)-oxirane-2,3-dicarboxylic acid: C191 (= C185), G192 (= G186), H193 (= H187), R228 (= R222), E285 (= E281), N313 (= N309), S315 (= S311), S317 (= S313), T347 (= T343)
6vb9A Covalent adduct of cis-2,3-epoxysuccinic acid with isocitrate lyase-1 from mycobacterium tuberculosis (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 22:427/427 of 6vb9A
- active site: Y89 (= Y82), D108 (= D101), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ C275), Q308 (≠ K304)
- binding oxalic acid: Y89 (= Y82), S91 (= S84), G92 (= G85), W93 (= W86), D153 (= D147), C191 (= C185), R228 (= R222), W283 (= W279), T347 (= T343)
5dqlA Crystal structure of 2-vinyl glyoxylate modified isocitrate lyase from mycobacterium tuberculosis (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 22:427/427 of 5dqlA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), C191 (= C185), H193 (= H187), R228 (= R222), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding magnesium ion: A276 (= A272), A279 (≠ C275), Q308 (≠ K304)
- binding 4-hydroxy-2-oxobutanoic acid: W93 (= W86), D108 (= D101), C191 (= C185), H193 (= H187), S315 (= S311), S317 (= S313), T347 (= T343), L348 (= L344)
6c4aA Crystal structure of 3-nitropropionate modified isocitrate lyase from mycobacterium tuberculosis with pyruvate (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 23:428/428 of 6c4aA
- active site: Y90 (= Y82), D109 (= D101), D154 (= D147), E156 (= E149), H181 (= H174), E183 (= E176), C192 (= C185), H194 (= H187), R229 (= R222), E286 (= E281), Q309 (≠ K304), S316 (= S311), S318 (= S313)
- binding 3-nitropropanoic acid: Y357 (= Y352), S358 (≠ G353), R380 (≠ A375)
- binding magnesium ion: A277 (= A272), A280 (≠ C275), Q309 (≠ K304)
- binding pyruvic acid: Y90 (= Y82), S92 (= S84), G93 (= G85), W94 (= W86), D154 (= D147), C192 (= C185), R229 (= R222), W284 (= W279), T348 (= T343)
P9WKK7 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
68% identity, 96% coverage: 15:424/429 of query aligns to 22:427/428 of P9WKK7
- SGW 91:93 (= SGW 84:86) binding substrate
- D153 (= D147) binding Mg(2+)
- C191 (= C185) mutation to S: Adopts a conformation almost identical to the wild-type.
- GH 192:193 (= GH 186:187) binding substrate
- R228 (= R222) binding substrate
- NCSPS 313:317 (= NCSPS 309:313) binding substrate
- K334 (≠ R330) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- T347 (= T343) binding substrate
6xppA Crystal structure of itaconate modified mycobaterium tuberculosis isocitrate lyase (see paper)
68% identity, 96% coverage: 15:424/429 of query aligns to 21:426/426 of 6xppA
- active site: Y88 (= Y82), D107 (= D101), D152 (= D147), E154 (= E149), H179 (= H174), E181 (= E176), C190 (= C185), H192 (= H187), R227 (= R222), E284 (= E281), Q307 (≠ K304), S314 (= S311), S316 (= S313)
- binding 2-methylidenebutanedioic acid: W92 (= W86), C190 (= C185), H192 (= H187), R227 (= R222), N312 (= N309), S314 (= S311), S316 (= S313), T346 (= T343)
- binding magnesium ion: A275 (= A272), A278 (≠ C275), Q307 (≠ K304)
1f8iA Crystal structure of isocitrate lyase:nitropropionate:glyoxylate complex from mycobacterium tuberculosis (see paper)
67% identity, 96% coverage: 15:424/429 of query aligns to 22:427/427 of 1f8iA
- active site: Y89 (= Y82), D108 (= D101), D153 (= D147), E155 (= E149), H180 (= H174), E182 (= E176), S191 (≠ C185), H193 (= H187), R228 (= R222), E285 (= E281), Q308 (≠ K304), S315 (= S311), S317 (= S313)
- binding glyoxylic acid: Y89 (= Y82), S91 (= S84), W93 (= W86), D153 (= D147), T347 (= T343)
P0A9G6 Isocitrate lyase; ICL; Isocitrase; Isocitratase; EC 4.1.3.1 from Escherichia coli (strain K12) (see 3 papers)
62% identity, 96% coverage: 15:424/429 of query aligns to 19:434/434 of P0A9G6
- SGW 91:93 (= SGW 84:86) binding substrate
- D157 (= D147) binding Mg(2+)
- C195 (= C185) active site, Proton acceptor; mutation to A: Large decrease in activity.; mutation to S: Large decrease in activity.
- A219 (= A209) mutation to C: Isocitrate lyase activity is reduced compared to the wild-type.
- R232 (= R222) binding substrate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1igwC Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
61% identity, 92% coverage: 15:407/429 of query aligns to 18:416/416 of 1igwC
- active site: Y88 (= Y82), D107 (= D101), D156 (= D147), E158 (= E149), H183 (= H174), E185 (= E176), C194 (= C185), R231 (= R222), E288 (= E281), K311 (= K304), S318 (= S311), S320 (= S313)
- binding pyruvic acid: S90 (= S84), G91 (= G85), W92 (= W86), D156 (= D147), R231 (= R222), T350 (= T343)
1igwA Crystal structure of the isocitrate lyase from the a219c mutant of escherichia coli (see paper)
58% identity, 92% coverage: 15:407/429 of query aligns to 18:396/396 of 1igwA
- active site: Y88 (= Y82), D107 (= D101), D156 (= D147), E158 (= E149), H183 (= H174), E185 (= E176), C194 (= C185), R227 (= R222), E284 (= E281), K307 (= K304)
- binding pyruvic acid: S90 (= S84), W92 (= W86), D156 (= D147), R227 (= R222), T330 (= T343)
5e9fD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
35% identity, 91% coverage: 15:406/429 of query aligns to 30:453/453 of 5e9fD
- active site: Y99 (= Y82), D118 (= D101), D172 (= D147), D174 (≠ E149), H199 (= H174), E201 (= E176), R240 (= R222), E330 (= E281), Q353 (≠ K304), S360 (= S311), S362 (= S313)
- binding magnesium ion: D118 (= D101), D172 (= D147)
5e9gD Structural insights of isocitrate lyases from magnaporthe oryzae (see paper)
34% identity, 92% coverage: 15:407/429 of query aligns to 31:486/486 of 5e9gD
- active site: Y100 (= Y82), D119 (= D101), D173 (= D147), D175 (≠ E149), H200 (= H174), E202 (= E176), C211 (= C185), H213 (= H187), R248 (= R222), E363 (= E281), Q386 (≠ K304), S393 (= S311), S395 (= S313)
- binding glyoxylic acid: Y100 (= Y82), S102 (= S84), G103 (= G85), W104 (= W86), D173 (= D147), H200 (= H174), R248 (= R222), T424 (= T343)
- binding glycerol: C211 (= C185), G212 (= G186), H213 (= H187), R248 (= R222)
6edwC Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
40% identity, 54% coverage: 13:243/429 of query aligns to 20:262/724 of 6edwC
Sites not aligning to the query:
6edwB Crystal structure of mycobacterium tuberculosis icl2 in the apo form (see paper)
40% identity, 54% coverage: 13:243/429 of query aligns to 20:262/746 of 6edwB
Sites not aligning to the query:
6edzA Crystal structure of mycobacterium tuberculosis icl2 in complex with acetyl-coa, form i (see paper)
40% identity, 54% coverage: 13:243/429 of query aligns to 20:262/733 of 6edzA
Sites not aligning to the query:
- active site: 437, 460, 467, 469
- binding acetyl coenzyme *a: 643, 644, 645, 646, 652, 653, 655, 657, 658, 679, 681, 682, 684, 688, 692, 732
7ebeA Crystal structure of isocitrate lyase-1 from candida albicans (see paper)
39% identity, 53% coverage: 15:243/429 of query aligns to 30:268/544 of 7ebeA
- active site: Y99 (= Y82), D118 (= D101), D172 (= D147), D174 (≠ E149), H199 (= H174), E201 (= E176), C210 (= C185), H212 (= H187), R247 (= R222)
- binding magnesium ion: G102 (= G85), W103 (= W86), D172 (= D147)
Sites not aligning to the query:
Query Sequence
>WP_012756106.1 NCBI__GCF_000023185.1:WP_012756106.1
MTDFYKLVPNALADRFDGIERPYSAEDVQRLRGSVALSHTLAAMGADRLWQLLHQEDFVN
ALGALSGNQAMQMVRAGLKAIYLSGWQVAADANTASAMYPDQSLYPANAGPELAKRINRT
LQRADQIETAEAQGLSVETWFAPIVADAEAGFGGPLNAFEIMKAYIEAGAAGVHFEDQLA
SEKKCGHLGGKVLIPTAAHIRNLDAARLAADVMGVATLVIARTDAEAAKLLTSDIDERDQ
PFVDYDAGRTVEGFYQVRNGIEPCIARAVAYAPHCDLIWCETSKPDLDQARRFAEGVHKV
HPGKLLAYNCSPSFNWKKNLDEATIARFQRELGAMGYKFQFITLAGFHQLNYGMYELARG
YRQRQMSAYSELQQAEFAAEVNGYTATKHQREVGTGYFDAVSLAITGGRSSTTAMHGSTE
HAQFKPAAE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory