SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012756940.1 NCBI__GCF_000023185.1:WP_012756940.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7kb1C Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
54% identity, 98% coverage: 7:426/427 of query aligns to 6:425/428 of 7kb1C

query
sites
7kb1C

G

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binding pyridoxal-5'-phosphate: Y57 (= Y57), R59 (= R59)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: G87 (= G87), Q88 (≠ H88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N370), I370 (= I371), R404 (= R405)

7kb1A Complex of o-acety-l-homoserine aminocarboxypropyltransferase (mety) from thermotoga maritima and a key reaction intermediate (see paper)
54% identity, 98% coverage: 7:426/427 of query aligns to 6:425/428 of 7kb1A

query
sites
7kb1A

G

G

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Y

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N

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T

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A

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A

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K

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y57 (= Y57), R59 (= R59), G87 (= G87), Q88 (≠ H88), Y112 (= Y112), N160 (= N159), D185 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), N369 (= N370), I370 (= I371), R404 (= R405)

8wkoA Crystal structure of o-acetylhomoserine sulfhydrylase from lactobacillus plantarum in the closed form (see paper)
51% identity, 98% coverage: 8:427/427 of query aligns to 9:425/425 of 8wkoA

query
sites
8wkoA

F

F

N

E

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L

A

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Y

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A

A

A

A

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D

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A

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G

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K

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A

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D

V

L

V

I

R

R

L

I

S

S

V

V

G

G

I

V

E

E

D

N

V

A

K

D

D

D

I

L

I

I

A

A

D

D

L

L

E

D

Q

Q

A

A

L

L

S

A

K

Q

I

V

binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: G87 (= G87), S88 (≠ H88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), S206 (= S206), A207 (≠ L207), T208 (= T208), F209 (= F210), G212 (= G213), M217 (= M218), V369 (≠ I371), A370 (≠ G372)
binding proline: H213 (= H214), Q284 (≠ T286), S288 (≠ T290)

2ctzA Crystal structure of o-acetyl homoserine sulfhydrylase from thermus thermophilus hb8
50% identity, 98% coverage: 8:425/427 of query aligns to 3:421/421 of 2ctzA

query
sites
2ctzA

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

Q

E

P

P

D

E

P

P

T

T

T

T

G

L

A

S

R

R

V

Q

T

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

A

S

F

Y

V

V

F

F

N

K

D

S

S

P

D

E

H

H

A

A

A

A

A

N

L

L

F

F

G

A

L

L

Q

K

A

E

F

F

G

G

N

N

I

I

Y

Y

T

S

R
|
R

I

I

M

M

N

N

P

P

T

T

Q

V

A

D

V

V

L

L

E

E

E

K

R

R

V

L

A

A

A

A

L

L

E

E

G

G

G

G

T

K

A

A

A

A

L

L

A

A

V

T

A

A

S
|
S

G
|
G

H
|
H

A

A

A

A

Q
|
Q

V

F

I

L

V

A

F

L

H

T

N

T

I

L

M

A

R

Q

P

A

G

G

E

D

N

N

F

I

I

V

A

S

A

T

R

P

Q

N

L

L

Y
|
Y

G

G

G

G

S

T

I

F

N

N

Q

Q

F

F

G

K

H

V

A

T

F

L

E

K

N

R

F

L

G

G

W

I

Q

E

V

V

R

R

W

F

A

T

D

S

A

R

A

E

D

E

-

R

P

P

A

E

S

E

F

F

E

L

S

A

Q

L

I

T

D

D

D

E

K

K

T

T

R

R

G

A

I

W

F

W

I

V

E

E

S

S

L

I

A

G

N

N

P

P

G

A

G

L

T

N

F

I

V

P

D

D

I

L

A

E

A

A

I

L

A

A

D

Q

V

A

A

A

H

R

R

E

H

K

G

G

L

V

P

A

L

L

I

I

V

V

D
|
D

N

N

T
|
T

M

F

A

G

T

M

P

G

-

G

Y

Y

F

L

I

L

R

R

P

P

I

L

E

A

H

W

G

G

A

A

D

A

I

L

V

V

V

T

H

H

S
|
S

L

L

T
|
T

K
|
K

F

W

L

V

G

G

G

G

H

H

G

G

N

A

S

V

M

I

G

A

G

G

L

A

I

I

V

V

D

D

G

G

G

G

T

N

F

F

D

P

W

W

S

-

K

E

S

G

G

G

N

R

Y

Y

P

P

M

L

L

L

S

T

S

E

P

P

R

Q

P

P

E

G

Y

Y

N

H

G

G

M

L

V

R

L

L

H

T

A

E

T

A

F

F

G

G

N

E

F

L

A

A

F

F

A

I

I

V

A

K

A

A

R

R

V

V

L

D

G

G

L

L

R

R

D

D

L

Q

G

G

P

Q

A

A

I

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

I

V

L

L

T

L

G

G

I

M

E

E

T

T

L

L

P

S

L

L

R

R

M

A

Q

E

R

R

H

H

S

V

D

E

N

N

A

T

I

L

A

H

V

L

A

A

K

H

W

W

L

L

K

L

A

E

H

Q

S

P

K

Q

I

V

A

A

W

W

V

V

N

N

Y

Y

A

P

G

G

L

L

D

P

D

H

D

H

P

P

N

H

H

H

A

D

L

R

Q

A

Q

Q

R

K

Y

Y

S

F

P

K

K

G

G

K

A

P

G

G

S

A

V

V

F

L

T

T

F

F

G

G

V

L

K

K

G

G

G

G

Y

Y

E

E

A

A

G

A

K

K

T

R

L

F

V

I

E

S

G

R

L

L

E

K

L

L

F

I

S

S

H

H

L

L

A

A

N

N

I

V

G

G

D

D

T

T

R

R

S

T

L

L

V

A

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

T

S

D

P

E

E

Q

E

R

Q

I

A

A

Q

A

A

G

G

A

V

G

S

P

P

G

E

V

M

V

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

V

V

K

E

D

D

I

L

I

K

A

A

D

E

L

L

E

K

Q

E

A

A

L

L

S

A

active site: R54 (= R59), Y107 (= Y112), D180 (= D184), K206 (= K209)
binding pyridoxal-5'-phosphate: S81 (= S86), G82 (= G87), H83 (= H88), Q86 (= Q91), Y107 (= Y112), D180 (= D184), T182 (= T186), S203 (= S206), T205 (= T208), K206 (= K209)

Q5SK88 O-acetyl-L-homoserine sulfhydrylase 1; OAH-sulfhydrylase 1; EC 2.5.1.- from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
50% identity, 98% coverage: 8:425/427 of query aligns to 3:421/421 of Q5SK88

query
sites
Q5SK88

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

Q

E

P

P

D

E

P

P

T

T

T

T

G

L

A

S

R

R

V

Q

T

V

P

P

I

I

Y

Y

Q

P

T

T

T

T

A

S

F

Y

V

V

F

F

N

K

D

S

S

P

D

E

H

H

A

A

A

A

A

N

L

L

F

F

G

A

L

L

Q

K

A

E

F

F

G

G

N

N

I

I

Y

Y

T

S

R

R

I

I

M

M

N

N

P

P

T

T

Q

V

A

D

V

V

L

L

E

E

E

K

R

R

V

L

A

A

A

A

L

L

E

E

G

G

G

G

T

K

A

A

A

A

L

L

A

A

V

T

A

A

S

S

G

G

H

H

A

A

A

A

Q

Q

V

F

I

L

V

A

F

L

H

T

N

T

I

L

M

A

R

Q

P

A

G

G

E

D

N

N

F

I

I

V

A

S

A

T

R

P

Q

N

L

L

Y

Y

G

G

G

G

S

T

I

F

N

N

Q

Q

F

F

G

K

H

V

A

T

F

L

E

K

N

R

F

L

G

G

W

I

Q

E

V

V

R

R

W

F

A

T

D

S

A

R

A

E

D

E

-

R

P

P

A

E

S

E

F

F

E

L

S

A

Q

L

I

T

D

D

D

E

K

K

T

T

R

R

G

A

I

W

F

W

I

V

E

E

S

S

L

I

A

G

N

N

P

P

G

A

G

L

T

N

F

I

V

P

D

D

I

L

A

E

A

A

I

L

A

A

D

Q

V

A

A

A

H

R

R

E

H

K

G

G

L

V

P

A

L

L

I

I

V

V

D

D

N

N

T

T

M

F

A

G

T

M

P

G

-

G

Y

Y

F

L

I

L

R

R

P

P

I

L

E

A

H

W

G

G

A

A

D

A

I

L

V

V

V

T

H

H

S

S

L

L

T

T

K
|
K

F

W

L

V

G

G

G

G

H

H

G

G

N

A

S

V

M

I

G

A

G

G

L

A

I

I

V

V

D

D

G

G

G

G

T

N

F

F

D

P

W

W

S

-

K

E

S

G

G

G

N

R

Y

Y

P

P

M

L

L

L

S

T

S

E

P

P

R

Q

P

P

E

G

Y

Y

N

H

G

G

M

L

V

R

L

L

H

T

A

E

T

A

F

F

G

G

N

E

F

L

A

A

F

F

A

I

I

V

A

K

A

A

R

R

V

V

L

D

G

G

L

L

R

R

D

D

L

Q

G

G

P

Q

A

A

I

L

S

G

P

P

F

F

N

E

A

A

F

W

L

V

I

V

L

L

T

L

G

G

I

M

E

E

T

T

L

L

P

S

L

L

R

R

M

A

Q

E

R

R

H

H

S

V

D

E

N

N

A

T

I

L

A

H

V

L

A

A

K

H

W

W

L

L

K

L

A

E

H

Q

S

P

K

Q

I

V

A

A

W

W

V

V

N

N

Y

Y

A

P

G

G

L

L

D

P

D

H

D

H

P

P

N

H

H

H

A

D

L

R

Q

A

Q

Q

R

K

Y

Y

S

F

P

K

K

G

G

K

A

P

G

G

S

A

V

V

F

L

T

T

F

F

G

G

V

L

K

K

G

G

G

G

Y

Y

E

E

A

A

G

A

K

K

T

R

L

F

V

I

E

S

G

R

L

L

E

K

L

L

F

I

S

S

H

H

L

L

A

A

N

N

I

V

G

G

D

D

T

T

R

R

S

T

L

L

V

A

I

I

H

H

P

P

A

A

S

S

T

T

T

T

H

H

R

S

Q

Q

L

L

T

S

D

P

E

E

Q

E

R

Q

I

A

A

Q

A

A

G

G

A

V

G

S

P

P

G

E

V

M

V

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

H

V

V

K

E

D

D

I

L

I

K

A

A

D

E

L

L

E

K

Q

E

A

A

L

L

S

A

K206 (= K209) modified: N6-(pyridoxal phosphate)lysine

O13326 Homocysteine synthase; O-acetylhomoserine sulfhydrylase; OAH SHL; OAH sulfhydrylase; EC 2.5.1.49 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
48% identity, 98% coverage: 8:425/427 of query aligns to 8:427/429 of O13326

query
sites
O13326

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Q

Q

E

P

P

D

D

P

A

T

A

T

T

G

S

A

S

R

R

V

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

T

A

S

F

Y

V

V

F

F

N

R

D

D

S

C

D

D

H

H

A

G

A

G

A

R

L

L

F

F

G

G

L

L

Q

Q

A

E

F

P

G

G

N

Y

I

I

Y

Y

T

S

R

R

I

M

M

M

N

N

P

P

T

T

Q

A

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

H

G

G

T

A

A

A

A

A

L

I

A

A

V

T

A

S

S

S

G

G

H

T

A

S

A

A

Q

L

V

F

I

M

V

A

F

L

H

T

N

T

I

L

M

A

R

K

P

A

G

G

E

D

N

N

F

I

I

V

A

S

A

T

R

S

Q

Y

L

L

Y

Y

G

G

G

G

S

T

I

Y

N

N

Q

L

F

F

G

K

H

V

A

T

F

L

E

P

N

R

F

L

G

G

W

I

Q

T

V

T

R

K

W

F

A

V

D

N

A

G

A

D

D

D

P

P

A

N

S

D

F

L

E

A

S

A

Q

Q

I

I

D

D

D

E

K

N

T

T

R

K

G

A

I

V

F

Y

I

V

E

E

S

S

L

I

A

G

N

N

P

P

G

M

G

Y

T

N

F

V

V

P

D

D

I

F

A

E

A

R

I

I

A

A

D

E

V

V

A

A

H

H

R

A

H

A

G

G

L

V

P

P

L

L

I

M

V

V

D

D

N

N

T

T

M

F

A

G

T

G

P

G

-

G

Y

Y

F

L

I

V

R

R

P

P

I

I

E

D

H

H

G

G

A

A

D

D

I

I

V

V

V

T

H

H

S

S

L

A

T

T

K

K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

M

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

K

F

F

D

D

W

W

S

K

K

K

-

N

S

S

G

K

N

R

Y

F

P

P

M

E

L

F

S

N

S

E

P

P

R

H

P

P

E

G

Y

Y

N

H

G

G

M

M

V

V

L

F

H

T

A

E

T

T

F

F

G

G

N

N

F

L

A

A

F

Y

A

A

I

F

A

A

A

C

R

R

V

T

L

Q

G

T

L

L

R

R

D

D

L

V

G

G

P

G

A

N

I

A

S

N

P

P

F

F

N

G

A

V

F

F

L

L

I

L

L

L

T

Q

G

G

I

L

E

E

T

T

L

L

P

S

L

L

R

R

M

M

Q

E

R

R

H

H

S

V

D

Q

N

N

A

A

I

F

A

A

V

L

A

A

K

K

W

Y

L

L

K

E

A

K

H

H

S

P

K

K

I

V

A

N

W

W

V

V

N

S

Y

Y

A

P

G

G

L

L

D

E

D

S

D

H

P

V

N

S

H

H

A

K

L

L

Q

A

Q

K

R

K

Y

Y

S

L

P

K

K

N

G

G

A

Y

G

G

S

A

V

V

F

L

T

S

F

F

G

G

V

A

K

K

G

G

G

G

Y

P

E

D

A

Q

G

S

K

R

T

K

L

V

V

V

E

N

G

A

L

L

E

K

L

L

F

A

S

S

H

Q

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

T

L

L

V

V

I

I

H

A

P

P

A

A

S

Y

T

T

T

T

H

H

R

L

Q

Q

L

L

T

T

D

D

E

E

Q

E

R

Q

I

I

A

S

A

A

G

G

A

V

G

T

P

K

G

D

V

L

V

I

R

R

L

V

S

A

V

V

G
|
G

I

I

E

E

D

H

V

I

K

D

D

D

I

I

I

I

A

A

D

D

L

F

E

A

Q

Q

A

A

L

L

S

E

G411 (= G409) mutation to D: Impairs homocysteine synthase activity.

8erbK Crystal structure of fub7 in complex with vinylglycine ketimine (see paper)
47% identity, 98% coverage: 8:426/427 of query aligns to 6:426/429 of 8erbK

query
sites
8erbK

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

Q

T

P

P

D

D

P

P

T

H

T

T

G

R

A

S

R

T

V

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

A

S

F

Y

V

T

F

F

N

N

D

D

S

S

D

A

H

H

A

G

A

A

A

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y
|
Y

T

S

R
|
R

I

L

M

M

N

N

P

P

T

T

Q

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

V

F

I

L

V

T

F

I

H

A

N

T

I

L

M

A

R

K

P

A

G

G

E

D

N

N

F

I

I

V

A

A

A

S

R

S

Q

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

G

N

H

V

A

L

F

L

E

P

N

R

F

F

G

G

W

I

Q

K

V

T

R

K

W

F

A

V

D

R

A

S

A

G

D

K

P

L

A

E

S

D

F

Y

E

A

S

A

Q

A

I

I

D

D

D

D

K

Q

T

T

R

R

G

A

I

I

F

Y

I

V

E

E

S

S

L

M

A

S

N
|
N

P

P

G

D

G

Y

T

V

F

V

V

P

D

D

I

F

A

E

A

G

I

I

A

A

D

K

V

I

A

A

H

H

R

E

H

H

G

G

L

I

P

P

L

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

F

Y

I

I

R

R

P

P

I

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

A

T
|
T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

M

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

D

N

W

W

S

N

K

K

-

H

S

S

G

D

N

R

Y

F

P

P

M

E

L

M

S

V

S

E

P

P

R

S

P

P

E

S

Y

Y

N

H

G

G

M

L

V

K

L

Y

H

W

A

E

T

A

F

F

G

G

N

P

F

A

A

T

F

F

A

I

I

T

A

R

A

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

I

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

I

E

A

K

V

L

A

S

K

K

W

Y

L

F

K

E

A

S

H

S

S

P

K

N

I

V

A

S

W

W

V

V

N

L

Y

W

A

P

G

G

L

S

D

E

D

S

D

H

P

P

N

T

H

Y

A

S

L

Q

Q

A

Q

K

R

K

Y

Y

S

L

P

T

K

R

G

G

A

F

G

G

S

A

V

M

F

L

T

S

F

I

G

G

V

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

K

S

T

K

L

V

V

V

E

D

G

G

L

L

E

K

L

L

F

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

R

R

I

L

A

A

A

S

G

G

A

V

G

T

P

E

G

D

V

M

V

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

V

V

K

D

D

D

I

I

I

I

A

A

D

D

L

F

E

E

Q

Q

A

S

L

F

S

Q

K

K

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]but-3-enoic acid: Y55 (= Y57), R57 (= R59), G85 (= G87), Q86 (≠ H88), Q89 (= Q91), Y110 (= Y112), N157 (= N159), D182 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), T385 (= T385), R405 (= R405)

8erjB Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
47% identity, 98% coverage: 8:426/427 of query aligns to 5:425/428 of 8erjB

query
sites
8erjB

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

Q

T

P

P

D

D

P

P

T

H

T

T

G

R

A

S

R

T

V

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

A

S

F

Y

V

T

F

F

N

N

D

D

S

S

D

A

H

H

A

G

A

A

A

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y

Y

T

S

R

R

I

L

M

M

N

N

P

P

T

T

Q

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S

S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

V

F

I

L

V

T

F

I

H

A

N

T

I

L

M

A

R

K

P

A

G

G

E

D

N

N

F

I

I

V

A

A

A

S

R

S

Q

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

G

N

H

V

A

L

F

L

E

P

N

R

F

F

G

G

W

I

Q

K

V

T

R

K

W

F

A

V

D

R

A

S

A

G

D

K

P

L

A

E

S

D

F

Y

E

A

S

A

Q

A

I

I

D

D

D

D

K

Q

T

T

R

R

G

A

I

I

F

Y

I

V

E

E

S

S

L

M

A

S

N

N

P

P

G

D

G

Y

T

V

F

V

V

P

D

D

I

F

A

E

A

G

I

I

A

A

D

K

V

I

A

A

H

H

R

E

H

H

G

G

L

I

P

P

L

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

F

Y

I

I

R

R

P

P

I

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

A

T

T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

M

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

D

N

W

W

S

N

K

K

-

H

S

S

G

D

N

R

Y

F

P

P

M

E

L

M

S

V

S

E

P

P

R

S

P

P

E

S

Y

Y

N

H

G

G

M

L

V

K

L

Y

H

W

A

E

T

A

F

F

G

G

N

P

F

A

A

T

F

F

A

I

I

T

A

R

A

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

I

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

I

E

A

K

V

L

A

S

K

K

W

Y

L

F

K

E

A

S

H

S

S

P

K

N

I

V

A

S

W

W

V

V

N

L

Y

W

A

P

G

G

L

S

D

E

D

S

D

H

P

P

N

T

H

Y

A

S

L

Q

Q

A

Q

K

R

K

Y

Y

S

L

P

T

K

R

G

G

A

F

G

G

S

A

V

M

F

L

T

S

F

I

G

G

V

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

K

S

T

K

L

V

V

V

E

D

G

G

L

L

E

K

L

L

F

V

S

S

H

N

L

L

A
|
A

N
|
N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T
|
T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

R

R

I

L

A

A

A

S

G

G

A

V

G

T

P

E

G

D

V

M

V

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

V

V

K

D

D

D

I

I

I

I

A

A

D

D

L

F

E

E

Q

Q

A

S

L

F

S

Q

K

K

binding (2S)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]but-3-enoic acid: G84 (= G87), Q85 (≠ H88), Q88 (= Q91), Y109 (= Y112), D181 (= D184), S204 (= S206), K207 (= K209), A368 (= A369), N369 (= N370), T384 (= T385), R404 (= R405)

8erjA Crystal structure of fub7 in complex with e-2-aminocrotonate (see paper)
47% identity, 98% coverage: 8:426/427 of query aligns to 5:425/428 of 8erjA

query
sites
8erjA

F

F

N

E

T

T

L

L

A

Q

I

L

H

H

A

A

G

G

A

Y

Q

T

P

P

D

D

P

P

T

H

T

T

G

R

A

S

R

T

V

A

T

V

P

P

I

I

Y

Y

Q

A

T

T

T

S

A

S

F

Y

V

T

F

F

N

N

D

D

S

S

D

A

H

H

A

G

A

A

A

R

L

L

F

F

G

G

L

L

Q

K

A

E

F

L

G

G

N

N

I

I

Y

Y

T

S

R

R

I

L

M

M

N

N

P

P

T

T

Q

V

A

D

V

V

L

F

E

E

E

K

R

R

V

I

A

A

A

A

L

L

E

E

G

G

G

G

T

I

A

A

A

A

L

A

A

A

V

T

A

S

S
|
S

G
|
G

H
x
Q

A

A

A

A

Q
|
Q

V

F

I

L

V

T

F

I

H

A

N

T

I

L

M

A

R

K

P

A

G

G

E

D

N

N

F

I

I

V

A

A

A

S

R

S

Q

H

L

L

Y
|
Y

G

G

G

G

S

T

I

Y

N

N

Q

Q

F

L

G

N

H

V

A

L

F

L

E

P

N

R

F

F

G

G

W

I

Q

K

V

T

R

K

W

F

A

V

D

R

A

S

A

G

D

K

P

L

A

E

S

D

F

Y

E

A

S

A

Q

A

I

I

D

D

D

D

K

Q

T

T

R

R

G

A

I

I

F

Y

I

V

E

E

S

S

L

M

A

S

N
|
N

P

P

G

D

G

Y

T

V

F

V

V

P

D

D

I

F

A

E

A

G

I

I

A

A

D

K

V

I

A

A

H

H

R

E

H

H

G

G

L

I

P

P

L

L

I

V

V

V

D
|
D

N

N

T

T

M

L

-

G

A

A

T

G

P

G

Y

Y

F

Y

I

I

R

R

P

P

I

I

E

E

H

H

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

A

T
|
T

K
|
K

F

W

L

I

G

G

G

G

H

H

G

G

N

T

S

T

M

I

G

G

G

G

L

V

I

I

V

V

D

D

G

S

G

G

T

R

F

F

D

N

W

W

S

N

K

K

-

H

S

S

G

D

N

R

Y

F

P

P

M

E

L

M

S

V

S

E

P

P

R

S

P

P

E

S

Y

Y

N

H

G

G

M

L

V

K

L

Y

H

W

A

E

T

A

F

F

G

G

N

P

F

A

A

T

F

F

A

I

I

T

A

R

A

I

R

R

V

V

L

E

G

M

L

L

R

R

D

D

L

I

G

G

P

A

A

C

I

L

S

S

P

P

F

F

N

S

A

A

F

Q

L

Q

I

L

L

L

T

L

G

G

I

I

E

E

T

T

L

L

P

G

L

L

R

R

M

A

Q

E

R

R

H

H

S

A

D

Q

N

N

A

T

I

E

A

K

V

L

A

S

K

K

W

Y

L

F

K

E

A

S

H

S

S

P

K

N

I

V

A

S

W

W

V

V

N

L

Y

W

A

P

G

G

L

S

D

E

D

S

D

H

P

P

N

T

H

Y

A

S

L

Q

Q

A

Q

K

R

K

Y

Y

S

L

P

T

K

R

G

G

A

F

G

G

S

A

V

M

F

L

T

S

F

I

G

G

V

V

K

K

G

G

G

D

Y

A

E

S

A

A

G

G

K

S

T

K

L

V

V

V

E

D

G

G

L

L

E

K

L

L

F

V

S

S

H

N

L

L

A

A

N

N

I

V

G

G

D

D

T

A

R

K

S

S

L

L

V

A

I

I

H

H

P

P

A

W

S

S

T

T

T

T

H

H

R

E

Q

Q

L

L

T

S

D

E

E

D

Q

E

R

R

I

L

A

A

A

S

G

G

A

V

G

T

P

E

G

D

V

M

V

I

R
|
R

L

I

S

S

V

V

G

G

I

I

E

E

D

H

V

V

K

D

D

D

I

I

I

I

A

A

D

D

L

F

E

E

Q

Q

A

S

L

F

S

Q

K

K

binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: S83 (= S86), G84 (= G87), Q85 (≠ H88), Q88 (= Q91), Y109 (= Y112), N156 (= N159), D181 (= D184), S204 (= S206), T206 (= T208), K207 (= K209), R404 (= R405)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 4hf8A

query
sites
4hf8A

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

R

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E
|
E

S

T

L

P

A

A

N
|
N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G87), I88 (≠ H88), Y112 (= Y112), E155 (= E155), N159 (= N159), D184 (= D184), S206 (= S206), K209 (= K209), S338 (≠ N370), R373 (= R405)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 4omaA

query
sites
4omaA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E

E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G87), I88 (≠ H88), Y112 (= Y112), D184 (= D184), S206 (= S206), T208 (= T208), K209 (= K209), V337 (≠ A369), S338 (≠ N370), R373 (= R405)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 3jwbA

query
sites
3jwbA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E

E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

L

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A

V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding norleucine: Y112 (= Y112), K209 (= K209), S338 (≠ N370), R373 (= R405)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 3jwaA

query
sites
3jwaA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E

E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

L

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (= Y112), K209 (= K209), V337 (≠ A369), S338 (≠ N370), R373 (= R405)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 3jw9A

query
sites
3jw9A

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G

G

H

I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E

E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S

S

L

V

T

T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R59 (= R59), Y112 (= Y112), D184 (= D184), K209 (= K209)
binding S-ethyl-L-cysteine: Y112 (= Y112), K209 (= K209), V337 (≠ A369), S338 (≠ N370)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 7:394/396 of 6egrA

query
sites
6egrA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R

R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E
|
E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T
|
T

M
x
F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A

V

N

S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

Y

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

binding pyridoxal-5'-phosphate: G87 (= G87), I88 (≠ H88), Y112 (= Y112), E155 (= E155), D184 (= D184), T186 (= T186), F187 (≠ M187), S206 (= S206), T208 (= T208), K209 (= K209)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
41% identity, 98% coverage: 7:426/427 of query aligns to 6:393/395 of 5m3zA

query
sites
5m3zA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

A

L

L

Q

E

A

E

F

S

G

G

N

Y

I

I

Y

Y

T

T

R
|
R

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G
x
H

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E
|
E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T
|
T

M

F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: R58 (= R59), Y111 (= Y112), D183 (= D184), K208 (= K209)
binding norleucine: Y111 (= Y112), H113 (≠ G114), K208 (= K209), V336 (≠ A369), S337 (≠ N370)
binding pyridoxal-5'-phosphate: G86 (= G87), I87 (≠ H88), Y111 (= Y112), E154 (= E155), D183 (= D184), T185 (= T186), S205 (= S206), T207 (= T208), K208 (= K209)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G87), I87 (≠ H88), Y111 (= Y112), D183 (= D184), S205 (= S206), T207 (= T208), K208 (= K209), V336 (≠ A369), S337 (≠ N370), R372 (= R405)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
39% identity, 98% coverage: 7:426/427 of query aligns to 7:383/386 of 3mkjA

query
sites
3mkjA

G

G

F

F

N

N

T

T

L

Q

A

I

I

V

H

H

A

A

G

G

A

Q

Q

Q

P

P

D

D

P

P

T

S

T

T

G

G

A

A

R

L

V

S

T

T

P

P

I

I

Y

F

Q

Q

T

T

T

S

A

T

F

F

V

V

F

F

N

D

D

S

S

A

D

E

H

Q

A

G

A

A

A

A

L

R

F

F

G

-

L

-

Q

-

A

-

F

-

G

-

N

-

I

-

Y

-

T

-

R

-

I

L

M

G

N

N

P

P

T

T

Q

T

A

D

V

A

L

L

E

E

E

K

R

K

V

L

A

A

A

V

L

L

E

E

G

R

G

G

T

E

A

A

A

G

L

L

A

A

V

T

A

A

S

S

G
|
G

H
x
I

A

S

A

A

Q

I

V

T

I

T

V

T

F

L

H

L

N

T

I

L

M

C

R

Q

P

Q

G

G

E

D

N

H

F

I

I

V

A

S

A

A

R

S

Q

A

L

I

Y
|
Y

G

G

G

C

S

T

I

H

N

A

Q

F

F

L

G

S

H

H

A

S

F

M

E

P

N

K

F

F

G

G

W

I

Q

N

V

V

R

S

W

F

A

V

D

D

A

A

A

A

D

K

P

P

A

E

S

E

F

I

E

R

S

A

Q

A

I

M

D

R

D

P

K

E

T

T

R

K

G

V

I

V

F

Y

I

I

E
|
E

S

T

L

P

A

A

N

N

P

P

G

T

G

L

T

S

F

L

V

V

D

D

I

I

A

E

A

T

I

V

A

A

D

G

V

I

A

A

H

H

R

Q

H

Q

G

G

L

A

P

L

L

L

I

V

V

V

D
|
D

N

N

T

T

M
x
F

A

M

T

S

P

P

Y

Y

F

C

I

Q

R

Q

P

P

I

L

E

Q

H

L

G

G

A

A

D

D

I

I

V

V

V

V

H

H

S
|
S

L

V

T
|
T

K
|
K

F

Y

L

I

G

N

G

G

H

H

G

G

N

D

S

V

M

I

G

G

G

G

L

I

I

I

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

K

F

Q

A

E

F

F

A

I

I

D

A

Q

A

A

R

R

V

F

L

V

G

G

L

L

R

K

D

D

L

I

-

T

G

G

P

G

A

C

I

M

S

S

P

P

F

F

N

N

A

A

F

W

L

L

I

T

L

L

T

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

M

M

Q

E

R

R

H

H

S

C

D

E

N

N

A

A

I

L

A

K

V

I

A

A

K

R

W

F

L

L

K

E

A

G

H

H

S

P

K

S

I

I

A

T

W

R

V

V

N

Y

Y

Y

A

P

G

G

L

L

D

S

D

S

D

H

P

P

N

Q

H

Y

A

E

L

L

Q

G

Q

Q

R

R

Y

Q

S

M

P

S

K

L

G

-

A

P

G

G

S

G

V

I

F

I

T

S

F

F

G

E

V

I

K

A

G

G

G

G

Y

L

E

E

A

A

G

G

K

R

T

R

L

M

V

I

E

N

G

S

L

V

E

E

L

L

F

C

S

L

H

L

L

A

A

V

N

S

I

L

G

G

D

D

T

T

R

E

S

T

L

L

V

I

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

P

L

V

T

A

D

P

E

E

Q

E

R

R

I

L

A

K

A

A

G

G

A

I

G

T

P

D

G

G

V

L

V

I

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

P

K

E

D

D

I

I

I

I

A

N

D

D

L

L

E

E

Q

H

A

A

L

I

S

R

K

K

active site: Y101 (= Y112), D173 (= D184), K198 (= K209)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G87), I77 (≠ H88), Y101 (= Y112), E144 (= E155), D173 (= D184), F176 (≠ M187), S195 (= S206), T197 (= T208), K198 (= K209)

1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
40% identity, 98% coverage: 6:425/427 of query aligns to 8:395/398 of 1pg8A

query
sites
1pg8A

P

P

G

G

F

F

N

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

Q

D

P

P

D

Q

P

D

T

H

T

G

G

G

A

A

R

L

V

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

A

T

F

F

V

T

F

F

N

P

D

T

S

V

D

E

H

Y

A

G

A

A

A

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

T

S

R
|
R

I

I

M

S

N

N

P

P

T

T

Q

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S
|
S

G
|
G

H
x
M

A

G

A

A

Q

I

V

T

I

S

V

T

F

L

H

W

N

T

I

L

M

L

R

R

P

P

G

G

E

D

N

E

F

V

I

L

A

L

A

G

R

N

Q

T

L

L

Y
|
Y

G

G

G

C

S

T

I

F

N

A

Q

F

F

L

G

H

H

H

A

G

F

I

E

G

N

E

F

F

G

G

W

V

Q

K

V

L

R

R

W

H

A

V

D

D

A

M

A

A

D

D

P

L

A

Q

S

A

F

L

E

E

S

A

Q

A

I

M

D

T

D

P

K

A

T

T

R

R

G

V

I

I

F

Y

I

F

E

E

S

S

L

P

A

A

N

N

P

P

G

N

G

M

T

H

F

M

V

A

D

D

I

I

A

A

A

G

I

V

A

A

D

K

V

I

A

A

H

R

R

K

H

H

G

G

L

A

P

T

L

V

I

V

V

V

D
|
D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

F

L

I

Q

R

R

P

P

I

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S
|
S

L

A

T
|
T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

M

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

S

F

Q

A

A

F

L

A

V

I

D

A

R

A

I

R

R

V

L

L

Q

G

G

L

L

R

K

D

D

L

M

-

T

G

G

P

A

A

V

I

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

T

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

I

Q

A

V

V

L

A

A

K

E

W

F

L

L

K

A

A

R

H

Q

S

P

K

Q

I

V

A

E

W

L

V

I

N

H

Y

Y

A

P

G

G

L

L

D

A

D

S

D

F

P

P

N

Q

H

Y

A

T

L

L

-

A

-

R

Q

Q

Q

Q

R

M

Y

S

S

Q

P

P

K

-

G

-

A

-

G

G

S

G

V

M

F

I

T

A

F

F

G

E

V

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

K

R

T

R

L

F

V

M

E

N

G

A

L

L

E

Q

L

L

F

F

S

S

H

R

L

A

A

V

N

S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

R

R

I

A

A

H

A

Y

G

G

A

I

G

S

P

E

G

G

V

L

V

V

R

R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

I

K

D

D

D

I

L

I

L

A

A

D

D

L

V

E

Q

Q

Q

A

A

L

L

S

K

active site: R61 (= R59), Y114 (= Y112), D186 (= D184), K211 (= K209)
binding pyridoxal-5'-phosphate: Y59 (= Y57), R61 (= R59), S88 (= S86), G89 (= G87), M90 (≠ H88), Y114 (= Y112), D186 (= D184), S208 (= S206), T210 (= T208), K211 (= K209)

P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
40% identity, 98% coverage: 6:425/427 of query aligns to 8:395/398 of P13254

query
sites
P13254

P

P

G

G

F

F

N

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

Q

D

P

P

D

Q

P

D

T

H

T

G

G

G

A

A

R

L

V

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

A

T

F

F

V

T

F

F

N

P

D

T

S

V

D

E

H

Y

A

G

A

A

A

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

T
x
S

R
|
R

I

I

M

S

N

N

P

P

T

T

Q

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S

S

G
|
G

H
x
M

A

G

A

A

Q

I

V

T

I

S

V

T

F

L

H

W

N

T

I

L

M

L

R

R

P

P

G

G

E

D

N

E

F

V

I

L

A

L

A

G

R

N

Q

T

L

L

Y
|
Y

G

G

G
x
C

S

T

I

F

N

A

Q

F

F

L

G

H

H

H

A

G

F

I

E

G

N

E

F

F

G

G

W

V

Q

K

V

L

R

R

W

H

A

V

D

D

A

M

A

A

D

D

P

L

A

Q

S

A

F

L

E

E

S

A

Q

A

I

M

D

T

D

P

K

A

T

T

R

R

G

V

I

I

F

Y

I

F

E

E

S

S

L

P

A

A

N

N

P

P

G

N

G

M

T

H

F

M

V

A

D

D

I

I

A

A

A

G

I

V

A

A

D

K

V

I

A

A

H

R

R

K

H

H

G

G

L

A

P

T

L

V

I

V

V

V

D

D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

F

L

I

Q

R

R

P

P

I

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S
|
S

L
x
A

T
|
T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

M

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

S

F

Q

A

A

F

L

A

V

I

D

A

R

A

I

R

R

V

L

L

Q

G

G

L

L

R
x
K

D
|
D

L

M

-

T

G

G

P

A

A

V

I

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

T

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

I

Q

A

V

V

L

A

A

K

E

W

F

L

L

K

A

A

R

H

Q

S

P

K

Q

I

V

A

E

W

L

V

I

N

H

Y

Y

A

P

G

G

L

L

D

A

D

S

D

F

P

P

N

Q

H

Y

A

T

L

L

-

A

-

R

Q

Q

Q

Q

R

M

Y

S

S

Q

P

P

K

-

G

-

A

-

G

G

S

G

V

M

F

I

T

A

F

F

G

E

V

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

K

R

T

R

L

F

V

M

E

N

G

A

L

L

E

Q

L

L

F

F

S

S

H

R

L

A

A

V

N

S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

R

R

I

A

A

H

A

Y

G

G

A

I

G

S

P

E

G

G

V

L

V

V

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

I

K

D

D

D

I

L

I

L

A

A

D

D

L

V

E

Q

Q

Q

A

A

L

L

S

K

YSR 59:61 (≠ YTR 57:59) binding pyridoxal 5'-phosphate
R61 (= R59) mutation R->A,E,F: Loss of elimination activity against L-methionine.
GM 89:90 (≠ GH 87:88) binding in other chain
Y114 (= Y112) binding substrate
C116 (≠ G114) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
SAT 208:210 (≠ SLT 206:208) binding in other chain
K211 (= K209) modified: N6-(pyridoxal phosphate)lysine
K240 (≠ R270) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
D241 (= D271) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
R375 (= R405) binding substrate

5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
40% identity, 98% coverage: 6:425/427 of query aligns to 2:389/392 of 5x2xA

query
sites
5x2xA

P

P

G

G

F

F

N

A

T

T

L

R

A

A

I

I

H

H

A

H

G

G

A

Y

Q

D

P

P

D

Q

P

D

T

H

T

G

G

G

A

A

R

L

V

V

T

P

P

P

I

V

Y

Y

Q

Q

T

T

T

A

A

T

F

F

V

T

F

F

N

P

D

T

S

V

D

E

H

Y

A

G

A

A

A

A

L

C

F

F

G

A

L

G

Q

E

A

Q

F

A

G

G

N

H

I

F

Y
|
Y

T

S

R
|
R

I

I

M

S

N

N

P

P

T

T

Q

L

A

N

V

L

L

L

E

E

E

A

R

R

V

M

A

A

A

S

L

L

E

E

G

G

G

G

T

E

A

A

A

G

L

L

A

A

V

L

A

A

S

S

G
|
G

H
x
M

A

G

A

A

Q

I

V

T

I

S

V

T

F

L

H

W

N

T

I

L

M

L

R

R

P

P

G

G

E

D

N

E

F

V

I

L

A

L

A

G

R

N

Q

T

L

L

Y
|
Y

G

G

G

C

S

T

I

F

N

A

Q

F

F

L

G

H

H

H

A

G

F

I

E

G

N

E

F

F

G

G

W

V

Q

K

V

L

R

R

W

H

A

V

D

D

A

M

A

A

D

D

P

L

A

Q

S

A

F

L

E

E

S

A

Q

A

I

M

D

T

D

P

K

A

T

T

R

R

G

V

I

I

F

Y

I

F

E

E

S

S

L

P

A

A

N
|
N

P

P

G

N

G

M

T

H

F

M

V

A

D

D

I

I

A

A

A

G

I

V

A

A

D

K

V

I

A

A

H

R

R

K

H

H

G

G

L

A

P

T

L

V

I

V

V

V

D
|
D

N

N

T

T

M

Y

A

C

T

T

P

P

Y

Y

F

L

I

Q

R

R

P

P

I

L

E

E

H

L

G

G

A

A

D

D

I

L

V

V

V

V

H

H

S
|
S

L

A

T
|
T

K
|
K

F

Y

L

L

G

S

G

G

H

H

G

G

N

D

S

I

M

T

G

A

G

G

L

I

I

V

V

V

D

-

G

-

G

-

T

-

F

-

D

-

W

-

S

-

K

-

S

-

G

-

N

-

Y

-

P

-

M

-

L

-

S

-

S

-

P

-

R

-

P

-

E

-

Y

-

N

-

G

-

M

-

V

-

L

-

H

-

A

-

T

-

F

-

G

G

N

S

F

Q

A

A

F

L

A

V

I

D

A

R

A

I

R

R

V

L

L

Q

G

G

L

L

R

K

D

D

L

M

-

T

G

G

P

A

A

V

I

L

S

S

P

P

F

H

N

D

A

A

F

A

L

L

I

L

L

M

T

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

M

M

Q

D

R

R

H

H

S

C

D

A

N

N

A

A

I

Q

A

V

V

L

A

A

K

E

W

F

L

L

K

A

A

R

H

Q

S

P

K

Q

I

V

A

E

W

L

V

I

N

H

Y

Y

A

P

G

G

L

L

D

A

D

S

D

F

P

P

N

Q

H

Y

A

T

L

L

-

A

-

R

Q

Q

Q

Q

R

M

Y

S

S

Q

P

P

K

-

G

-

A

-

G

G

S

G

V

M

F

I

T

A

F

F

G

E

V

L

K

K

G

G

G

G

Y

I

E

G

A

A

G

G

K

R

T

R

L

F

V

M

E

N

G

A

L

L

E

Q

L

L

F

F

S

S

H

R

L

A

A
x
V

N
x
S

I

L

G

G

D

D

T

A

R

E

S

S

L

L

V

A

I

Q

H

H

P

P

A

A

S

S

T

M

T

T

H

H

R

S

Q

S

L

Y

T

T

D

P

E

E

Q

E

R

R

I

A

A

H

A

Y

G

G

A

I

G

S

P

E

G

G

V

L

V

V

R
|
R

L

L

S

S

V

V

G

G

I

L

E

E

D

D

V

I

K

D

D

D

I

L

I

L

A

A

D

D

L

V

E

Q

Q

Q

A

A

L

L

S

K

active site: R55 (= R59), Y108 (= Y112), D180 (= D184), K205 (= K209)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y57), R55 (= R59), G83 (= G87), M84 (≠ H88), Y108 (= Y112), N155 (= N159), D180 (= D184), S202 (= S206), T204 (= T208), K205 (= K209), V333 (≠ A369), S334 (≠ N370), R369 (= R405)

Query Sequence

>WP_012756940.1 NCBI__GCF_000023185.1:WP_012756940.1
MAKNDPGFNTLAIHAGAQPDPTTGARVTPIYQTTAFVFNDSDHAAALFGLQAFGNIYTRI
MNPTQAVLEERVAALEGGTAALAVASGHAAQVIVFHNIMRPGENFIAARQLYGGSINQFG
HAFENFGWQVRWADAADPASFESQIDDKTRGIFIESLANPGGTFVDIAAIADVAHRHGLP
LIVDNTMATPYFIRPIEHGADIVVHSLTKFLGGHGNSMGGLIVDGGTFDWSKSGNYPMLS
SPRPEYNGMVLHATFGNFAFAIAARVLGLRDLGPAISPFNAFLILTGIETLPLRMQRHSD
NAIAVAKWLKAHSKIAWVNYAGLDDDPNHALQQRYSPKGAGSVFTFGVKGGYEAGKTLVE
GLELFSHLANIGDTRSLVIHPASTTHRQLTDEQRIAAGAGPGVVRLSVGIEDVKDIIADL
EQALSKI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory