SitesBLAST

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
27% identity, 96% coverage: 3:530/552 of query aligns to 94:636/667 of P09342

query
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P09342

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C161 (= C69) modified: Disulfide link with 307
P194 (≠ Q102) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ R209) modified: Disulfide link with 161

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
27% identity, 96% coverage: 3:530/552 of query aligns to 91:633/664 of P09114

query
sites
P09114

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P191 (≠ Q102) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (≠ H473) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

5d6rB Acetolactate synthase from klebsiella pneumoniae in complex with mechanism-based inhibitor
27% identity, 96% coverage: 5:533/552 of query aligns to 7:534/548 of 5d6rB

query
sites
5d6rB

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active site: I26 (≠ V24), G28 (= G26), A29 (≠ E27), K30 (≠ S28), I31 (≠ F29), E51 (= E49), T74 (= T72), H113 (≠ F111), Q114 (= Q112), S115 (≠ E113), Q163 (≠ E161), L254 (≠ I252), E281 (≠ P279), M386 (≠ A385), Q412 (≠ G410), M414 (= M412), D439 (= D437), D466 (≠ N463), G468 (= G465), Y469 (= Y467), M471 (≠ T469), V472 (≠ I470), Q475 (≠ H473)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(Z)-2-fluoro-1-hydroxy-2-phosphonoethenyl]-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: M386 (≠ A385), G387 (= G386), S388 (≠ N387), Q412 (≠ G410), M414 (= M412), D439 (= D437), G440 (= G438), G468 (= G465), Y469 (= Y467), N470 (≠ G468), M471 (≠ T469)
binding magnesium ion: R63 (= R61), Q212 (≠ D211), D439 (= D437), D466 (≠ N463), G468 (= G465)

Sites not aligning to the query:

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (≠ I252), R292 (≠ M278), W489 (≠ H473)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), G426 (= G410), M428 (= M412), G452 (= G436), D453 (= D437), G454 (= G438), S455 (≠ C439), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), M263 (≠ D249), L264 (≠ V250), M266 (≠ I252), H267 (≠ G253), G286 (= G272), R288 (= R274), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408)
binding magnesium ion: A37 (≠ S28), T82 (= T72), S83 (≠ R73), Q122 (= Q112), Y381 (vs. gap), D453 (= D437), M458 (= M442), Q461 (= Q445), N480 (= N464), H482 (≠ I466)

Sites not aligning to the query:

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
29% identity, 88% coverage: 3:490/552 of query aligns to 97:591/670 of P17597

query
sites
P17597

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I

Y
x
D

P
x
I

D

D

A

S

S

A

E

E

L

I

G

G

R

K

I

N

Y

K

R

T

P

P

D

H

L

V

A

S

I

V

C

C

A

-

P

-

A

G

D
|
D

F
x
V

V

K

A

L

A

A

L

L

A

Q

D

G

-

M

-

N

-

K

-

V

L

L

E

E

A

N

P

R

A

A

E

E

P

E

L

L

W

K

A

L

E

D

R

F

T

G

A

V

R

W

L

R

H

N

Q

E

A

L

Y

N

L

V

A

Q

W

K

S

Q

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K

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F

P

P

L

L

S

S

G

F

P

K

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A

F

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M

A

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-

P

-

Q

-

Y

I

A

M

I

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L

E

D

A

E

N

L

T

T

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D

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K

T

A

I

I

F

I

T

S

N

T

G

G

A

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G

N
x
Q

Y
x
H

A

Q

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M

W

W

V

A

H

A

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F

F

H

Y

R

N

F

Y

R

K

F

P

N

R

T

Q

Q

W

A

L

A

S

P

S

T
x
G

S
x
G

-

L

G
|
G

S
x
A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

V

I

A

G

A

A

K

S

R

V

L

A

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N

P

P

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R

A

E

I

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V

I

V

C

D

F

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A

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G

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D

G
|
G

C
x
S

F

F

L

I

M

M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

L

N

N

N
|
N

G
x
Q

I
x
H

Y
x
L

G
|
G

T
x
M

I

V

R

M

M

Q

H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

A

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V

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A

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L

L

T

G

N

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P

A122 (≠ S28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E49) binding thiamine diphosphate
S186 (= S91) binding FAD
P197 (≠ Q102) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ E104) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q112) binding thiamine diphosphate
K220 (≠ E122) binding (R)-imazaquin
R246 (= R151) binding (R)-imazaquin; binding FAD
K256 (≠ E161) binding chlorimuron-ethyl
G308 (= G207) binding FAD
T----L 331:332 (≠ SFRRQM 232:237) binding FAD
C340 (= C245) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ VGIG 250:253) binding FAD
GVRFD 371:375 (≠ GSRMS 272:276) binding FAD
DR 376:377 (≠ EM 277:278) binding chlorimuron-ethyl
DI 395:396 (≠ YP 299:300) binding FAD
DV 414:415 (≠ DF 321:322) binding FAD
QH 487:488 (≠ NY 387:388) binding thiamine diphosphate
GG 508:509 (≠ TS 408:409) binding FAD
GAM 511:513 (≠ GSM 410:412) binding thiamine diphosphate
D538 (= D437) binding Mg(2+)
DGS 538:540 (≠ DGC 437:439) binding thiamine diphosphate
N565 (= N464) binding Mg(2+)
NQHLGM 565:570 (≠ NGIYGT 464:469) binding thiamine diphosphate
H567 (≠ I466) binding Mg(2+)
W574 (≠ H473) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.

Sites not aligning to the query:

653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/585 of 5k2oA

query
sites
5k2oA

K

R

T

K

G

G

G

A

E

D

L

I

I

L

V

V

E

E

A

A

L

L

K

E

A

R

N

Q

G

G

V

V

K

E

R

T

L

V

S

F

C

A

V
x
Y

P

P

G
|
G

E
x
G

S
x
A

F
x
S

L

M

A

E

V

I

L

H

D

Q

A

A

L

L

-

T

R

R

D

S

S

S

D

S

I

I

D

R

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N

L

V

V

L

C

P

R

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E
|
E

G

Q

G

G

A

G

A

V

M

F

M

A

A

A

D

E

C

G

W

Y

G

A

R

R

L

S

T

S

G

G

E

K

P

P

G

G

I

I

C

C

M

I

V

A

T
|
T

R

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

S

V

A

S

G

G

L

L

H

A

I

D

A

A

K

L

Q

L

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S

I

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M

L

I

V

L

A

F

I

I

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G

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R

A

M

R

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A

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F

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E
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E

-

T

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-

I

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E

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I

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E

R

E

A

A

F

F

A

F

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L

A

A

T

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

L

V

T

D

L

V

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P

E
x
K

D

D

M

I

L

Q

R

Q

D

Q

E

L

V

A

-

I

-

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-

N

-

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-

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Y

Y

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R

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L

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x
G

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L

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x
M

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A

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L

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L

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G

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R

M

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x
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M
x
R

P
x
V

S

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S

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D

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x
V

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A

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A

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A

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D

G

-

M

-

N

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K

-

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x
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x
M

W

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V

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F

F

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Y

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A

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x
G

S

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-

L

G
|
G

S

A

M
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M

G

G

Y

F

G

G

L

L

P

P

A

A

V

I

A

G

A

A

K

S

R

V

L

A

F

N

P

P

E

D

R

A

E

I

V

V

I

V

C

D

F

I

A

D

G

G

D
|
D

G
|
G

C
x
S

F

F

L

I

M

M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

L

N

N

N
|
N

G

Q

I
x
H

Y
x
L

G
|
G

T
x
M

I
x
V

R

M

M

Q

H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

A

R

N

V

R

S

A

S

H

T

T

D

F

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L

T

G

N

D

P

P

active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (≠ I252), R292 (≠ M278), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), G286 (= G272), R288 (= R274), D290 (≠ S276), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), Q404 (≠ A389), M405 (≠ T390), G423 (≠ T408)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), M428 (= M412), D453 (= D437), G454 (= G438), S455 (≠ C439), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)

Sites not aligning to the query:

active site: 558
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: 568

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 11:505/582 of 3ea4A

query
sites
3ea4A

K

R

T

K

G

G

G

A

E

D

L

I

I

L

V

V

E

E

A

A

L

L

K

E

A

R

N

Q

G

G

V

V

K

E

R

T

L

V

S

F

C

A

V
x
Y

P

P

G
|
G

E
x
G

S
x
A

F
x
S

L

M

A

E

V

I

L

H

D

Q

A

A

L

L

-

T

R

R

D

S

S

S

D

S

I

I

D

R

V

N

L

V

V

L

C

P

R

R

Q

H

E
|
E

G

Q

G

G

A

G

A

V

M

F

M

A

A

A

D

E

C

G

W

Y

G

A

R

R

L

S

T

S

G

G

E

K

P

P

G

G

I

I

C

C

M

I

V

A

T
|
T

R

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

S

V

A

S

G

G

L

L

H

A

I

D

A

A

K

L

Q

L

D

D

S

S

I

V

P

P

M

L

I

V

L

A

F

I

I

T

G

G

Q

Q

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V

Q

P

R

R

E

R

A

M

R

I

E

G

R

T

E

D

A

A

F
|
F

Q
|
Q

E
|
E

-

T

-

P

-

I

V

V

E

E

F

V

R

T

R

R

A

S

F

I

T

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E

K

F

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A

N

K

Y

W

L

V

V

G

M

E

D

I

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D

E

D

D

A

-

R

-

I

I

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P

E

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F

I

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I

T

E

R

E

A

A

F

F

A

F

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A

A

T

T

S

S

G

G

R
|
R

P

P

G

G

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L

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D

L

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x
K

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A

-

I

-

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V

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G

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x
G

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L

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N

A

S

D

S

A

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V

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A

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A

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G

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M
x
R

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x
V

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x
V

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A

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A

A

L

L

A

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D

G

-

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-

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-

K

-

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L

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K

A

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P

F

P

P

L

L

S

S

G

F

P

K

G

T

A

F

V

G

H

E

M

A

G

I

P

P

-

P

-

Q

-

Y

I

A

M

I

E

K

W

V

L

L

E

D

A

E

N

L

T

T

G

D

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A

I

I

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I

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S

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T

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x
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x
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x
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A

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x
M

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W

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A

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F

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Y

R

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x
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|
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G

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L

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P

A

A

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I

A

G

A

A

K

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R

V

L

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P

P

E

D

R

A

E

I

V

V

I

V

C

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F

I

A

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|
G

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G
|
G

C
x
S

F

F

L

I

M

M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

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P

I

V

I

K

A

V

V

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N

N

N
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N

G

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x
H

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x
L

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x
M

I
x
V

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M

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H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

A

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N

V

R

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S

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T

D

F

L

L

T

G

N

D

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P

active site: Y32 (≠ V24), G34 (= G26), G35 (≠ E27), A36 (≠ S28), S37 (≠ F29), E58 (= E49), T81 (= T72), F120 (= F111), Q121 (= Q112), E122 (= E113), K170 (≠ E161), M265 (≠ I252), V292 (≠ P279), V399 (≠ A385), G425 (= G410), M427 (= M412), D452 (= D437), N479 (= N464), H481 (≠ I466), L482 (≠ Y467), M484 (≠ T469), V485 (≠ I470), W488 (≠ H473)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ E277), R291 (≠ M278), W488 (≠ H473)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R151), G221 (= G206), G222 (= G207), G223 (≠ T208), T245 (≠ S232), L246 (≠ F233), M247 (≠ R234), L263 (≠ V250), G264 (= G251), M265 (≠ I252), H266 (≠ G253), G285 (= G272), R287 (= R274), D289 (≠ S276), R291 (≠ M278), D309 (≠ Y299), I310 (≠ P300), G327 (≠ A320), D328 (= D321), V329 (≠ F322), M404 (≠ T390), G422 (≠ T408)
binding magnesium ion: D452 (= D437), N479 (= N464), H481 (≠ I466)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ A385), G400 (= G386), Q401 (≠ N387), H402 (≠ Y388), M427 (= M412), G451 (= G436), D452 (= D437), G453 (= G438), S454 (≠ C439), N479 (= N464), H481 (≠ I466), L482 (≠ Y467), G483 (= G468), M484 (≠ T469), V485 (≠ I470)

Sites not aligning to the query:

active site: 557
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 11:505/582 of 3e9yA

query
sites
3e9yA

K

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K

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G

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A

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I

I

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P

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|
G

E
x
G

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x
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R

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G

G

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C

C

M

I

V

A

T
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T

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G

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A

A

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N

N

A

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A

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G

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A

A

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V

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R

A

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I

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V

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G

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G
|
G

C
x
S

F

F

L

I

M

M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

L

N

N

N
|
N

G

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I
x
H

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x
L

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|
G

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x
M

I
x
V

R

M

M

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x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

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H

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F

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L

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P

active site: Y32 (≠ V24), G34 (= G26), G35 (≠ E27), A36 (≠ S28), S37 (≠ F29), E58 (= E49), T81 (= T72), F120 (= F111), Q121 (= Q112), E122 (= E113), K170 (≠ E161), M265 (≠ I252), V292 (≠ P279), V399 (≠ A385), G425 (= G410), M427 (= M412), D452 (= D437), N479 (= N464), H481 (≠ I466), L482 (≠ Y467), M484 (≠ T469), V485 (≠ I470), W488 (≠ H473)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ E277), R291 (≠ M278), W488 (≠ H473)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R151), G221 (= G206), G222 (= G207), G223 (≠ T208), T245 (≠ S232), L246 (≠ F233), M247 (≠ R234), L263 (≠ V250), G285 (= G272), R287 (= R274), D289 (≠ S276), R291 (≠ M278), D309 (≠ Y299), I310 (≠ P300), G327 (≠ A320), D328 (= D321), V329 (≠ F322), M404 (≠ T390), G422 (≠ T408)
binding magnesium ion: D452 (= D437), N479 (= N464), H481 (≠ I466)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ A385), G400 (= G386), Q401 (≠ N387), H402 (≠ Y388), M427 (= M412), G451 (= G436), G453 (= G438), S454 (≠ C439), N479 (= N464), H481 (≠ I466), L482 (≠ Y467), G483 (= G468), M484 (≠ T469), V485 (≠ I470)

Sites not aligning to the query:

active site: 557
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 567

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 8et4A

query
sites
8et4A

K

R

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K

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G

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I

I

L

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V

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E

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A

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G

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N

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G

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D

F

I

A

D

G
|
G

D
|
D

G
|
G

C
x
S

F

F

L

I

M
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M

H

N

G

V

Q
|
Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

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N

N

N
|
N

G

Q

I
x
H

Y
x
L

G
|
G

T
x
M

I
x
V

R

M

M

Q

H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

A

R

N

V

R

S

A

S

H

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T

D

F

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L

T

G

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D

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P

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), G426 (= G410), M428 (= M412), G452 (= G436), D453 (= D437), G454 (= G438), S455 (≠ C439), M458 (= M442), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), M266 (≠ I252), H267 (≠ G253), G286 (= G272), V287 (≠ S273), R288 (= R274), D290 (≠ S276), R292 (≠ M278), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408)
binding magnesium ion: F370 (≠ G358), D453 (= D437), M458 (= M442), Q461 (= Q445), N480 (= N464), H482 (≠ I466)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ I252), R292 (≠ M278), M485 (≠ T469), W489 (≠ H473)

Sites not aligning to the query:

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 5wj1A

query
sites
5wj1A

K

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x
H

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x
L

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|
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x
M

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x
V

R

M

M

Q

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x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

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active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), M263 (≠ D249), L264 (≠ V250), G286 (= G272), R288 (= R274), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408), G424 (≠ S409)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ I252), D291 (≠ E277), R292 (≠ M278), M485 (≠ T469), W489 (≠ H473)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), M428 (= M412), D453 (= D437), G454 (= G438), S455 (≠ C439), M458 (= M442), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 5k6tA

query
sites
5k6tA

K

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K

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G

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D

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G

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|
G

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x
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x
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x
W

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K

G

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active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ G253), R292 (≠ M278), M485 (≠ T469), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), G286 (= G272), R288 (= R274), D290 (≠ S276), R292 (≠ M278), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), Q404 (≠ A389), M405 (≠ T390), G423 (≠ T408)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), G426 (= G410), M428 (= M412), G452 (= G436), G454 (= G438), S455 (≠ C439), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 5k6rA

query
sites
5k6rA

K

R

T

K

G

G

G

A

E

D

L

I

I

L

V

V

E

E

A

A

L

L

K

E

A

R

N

Q

G

G

V

V

K

E

R

T

L

V

S

F

C

A

V
x
Y

P

P

G
|
G

E
x
G

S
x
A

F
x
S

L

M

A

E

V

I

L

H

D

Q

A

A

L

L

-

T

R

R

D

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I

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L

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V

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C

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E
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E

G

Q

G

G

A

G

A

V

M

F

M

A

A

A

D

E

C

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W

Y

G

A

R

R

L

S

T

S

G

G

E

K

P

P

G

G

I

I

C

C

M

I

V

A

T
|
T

R

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

S

V

A

S

G

G

L

L

H

A

I

D

A

A

K

L

Q

L

D

D

S

S

I

V

P

P

M

L

I

V

L

A

F

I

I

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Q

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V

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E

R

A

M

R

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D

A

A

F
|
F

Q
|
Q

E
|
E

-

T

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P

-

I

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E

E

F

V

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T

R

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A

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F

I

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E

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D

A

-

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-

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R

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x
K

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-

I

-

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-

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-

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x
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-

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x
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M

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x
R

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x
V

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E

L

A

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N

-

I

-

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Y

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K

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H

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x
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x
I

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A

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A

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G

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x
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x
V

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K

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A

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A

Q

D

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-

M

-

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-

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-

V

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A

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-

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-

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A

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I

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S

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x
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G

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x
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x
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M

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Y

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K

F

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T

Q

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W

A

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x
G

S

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-

L

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A

M
|
M

G

G

Y

F

G

G

L

L

P

P

A

A

V

I

A

G

A

A

K

S

R

V

L

A

F

N

P

P

E

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R

A

E

I

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V

I

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C

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F

I

A

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G

G

D
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D

G
|
G

C
x
S

F

F

L

I

M
|
M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

L

N

N

N
|
N

G

Q

I
x
H

Y
x
L

G
|
G

T
x
M

I
x
V

R

M

M

Q

H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

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V

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P

active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (≠ M278), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), M266 (≠ I252), G286 (= G272), R288 (= R274), R292 (≠ M278), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), G328 (≠ A320), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), G426 (= G410), M428 (= M412), D453 (= D437), G454 (= G438), S455 (≠ C439), M458 (= M442), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 1z8nA

query
sites
1z8nA

K

R

T

K

G

G

G

A

E

D

L

I

I

L

V

V

E

E

A

A

L

L

K

E

A

R

N

Q

G

G

V

V

K

E

R

T

L

V

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F

C

A

V
x
Y

P

P

G
|
G

E
x
G

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x
A

F
x
S

L

M

A

E

V

I

L

H

D

Q

A

A

L

L

-

T

R

R

D

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S

S

D

S

I

I

D

R

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L

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V

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E

G

Q

G

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G

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M

A

A

A

D

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C

G

W

Y

G

A

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R

L

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S

G

G

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K

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P

G

G

I

I

C

C

M

I

V

A

T
|
T

R

S

G

G

P

P

G

G

A

A

T

T

N

N

A

L

S

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A

S

G

G

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A

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A

A

K

L

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L

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G

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Q

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E

G

R

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E

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A

A

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F

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Q

E
|
E

-

T

-

P

-

I

V

V

E

E

F

V

R

T

R

R

A

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F

I

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E
x
K

F

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A

N

K

Y

W

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V

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M

E

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I

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D

E

D

D

A

-

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-

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E

R

E

A

A

F

F

A

F

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A

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S

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R

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G

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x
K

D

D

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Y

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G

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x
G

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x
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x
V

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E

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-

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M

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A

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Y

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F

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W

A

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P

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x
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x
G

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|
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|
M

G

G

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F

G

G

L

L

P

P

A

A

V

I

A

G

A

A

K

S

R

V

L

A

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N

P

P

E

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A

E

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V

V

I

V

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F

I

A

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|
G

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G
|
G

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x
S

F

F

L

I

M

M

H

N

G

V

Q

Q

E

E

F

L

A

A

T

T

A

I

I

R

R

V

Y

E

G

N

L

L

P

P

I

V

I

K

A

V

V

L

N

N

N
|
N

G

Q

I
x
H

Y
x
L

G
|
G

T
x
M

I
x
V

R

M

M

Q

H
x
W

Q

E

E

D

R

R

E

F

Y

Y

P

K

G

A

R

N

V

R

S

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S

H

T

T

D

F

L

L

T

G

N

D

P

P

active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (≠ E122), R161 (= R151), Y191 (= Y176), R194 (≠ V179), D291 (≠ E277), R292 (≠ M278), D312 (= D301), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), G265 (= G251), M266 (≠ I252), H267 (≠ G253), G286 (= G272), V287 (≠ S273), R288 (= R274), D290 (≠ S276), R292 (≠ M278), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408), G424 (≠ S409)
binding magnesium ion: D453 (= D437), N480 (= N464)
binding thiamine diphosphate: V400 (≠ A385), G401 (= G386), Q402 (≠ N387), H403 (≠ Y388), G426 (= G410), M428 (= M412), G452 (= G436), G454 (= G438), S455 (≠ C439), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), G484 (= G468), M485 (≠ T469), V486 (≠ I470)

Sites not aligning to the query:

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 1yi1A

query
sites
1yi1A

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active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ E277), R292 (≠ M278), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), M263 (≠ D249), L264 (≠ V250), G265 (= G251), M266 (≠ I252), H267 (≠ G253), G286 (= G272), V287 (≠ S273), R288 (= R274), D290 (≠ S276), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408), G424 (≠ S409)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)

Sites not aligning to the query:

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
29% identity, 88% coverage: 3:490/552 of query aligns to 12:506/582 of 1yi0A

query
sites
1yi0A

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active site: Y33 (≠ V24), G35 (= G26), G36 (≠ E27), A37 (≠ S28), S38 (≠ F29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (≠ E161), M266 (≠ I252), V293 (≠ P279), V400 (≠ A385), G426 (= G410), M428 (= M412), D453 (= D437), N480 (= N464), H482 (≠ I466), L483 (≠ Y467), M485 (≠ T469), V486 (≠ I470), W489 (≠ H473)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ E277), R292 (≠ M278), W489 (≠ H473)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G206), G223 (= G207), G224 (≠ T208), T246 (≠ S232), L247 (≠ F233), M248 (≠ R234), L264 (≠ V250), G265 (= G251), M266 (≠ I252), H267 (≠ G253), G286 (= G272), V287 (≠ S273), R288 (= R274), D290 (≠ S276), R292 (≠ M278), V293 (≠ P279), D310 (≠ Y299), I311 (≠ P300), G328 (≠ A320), D329 (= D321), V330 (≠ F322), M405 (≠ T390), G423 (≠ T408), G424 (≠ S409)
binding magnesium ion: D453 (= D437), N480 (= N464), H482 (≠ I466)

Sites not aligning to the query:

Query Sequence

>WP_012758316.1 NCBI__GCF_000023185.1:WP_012758316.1
MKKTGGELIVEALKANGVKRLSCVPGESFLAVLDALRDSDIDVLVCRQEGGAAMMADCWG
RLTGEPGICMVTRGPGATNASAGLHIAKQDSIPMILFIGQVQREAREREAFQEVEFRRAF
TEFAKWVGEIDDAARIPEFVTRAFAVATSGRPGPVVLTLPEDMLRDEVEAPRAKHYASVE
AHPGRRQIDDFYLRLLKAERPMVILGGTRWDADAVADFQSFAERFQLPVGCSFRRQMLFD
HLHPCYAGDVGIGINPALAKEIKESDLLILLGSRMSEMPSSSYTLINIPYPQQSLVHIYP
DASELGRIYRPDLAICAAPADFVAALADLEAPAEPLWAERTARLHQAYLAWSKPPLSGPG
AVHMGPIMEWLEANTGPQTIFTNGAGNYATWVHRFHRFRRFNTQAAPTSGSMGYGLPAAV
AAKRLFPEREVICFAGDGCFLMHGQEFATAIRYGLPIIAVVVNNGIYGTIRMHQEREYPG
RVSSTDLTNPDFAALARAYGGHGETVERTEEFAPAFERARASGKPAIIEVKLDPEAITPT
RTLSEIAQTKSR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory