SitesBLAST
Comparing WP_012759040.1 NCBI__GCF_000023185.1:WP_012759040.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
51% identity, 98% coverage: 8:487/488 of query aligns to 2:472/488 of 8vc5A
2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 2cv2A
- active site: K246 (= K260)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R13), A7 (= A15), S9 (= S17), G17 (= G25), I21 (= I29), E41 (= E49), Y187 (= Y201), R205 (= R219), A206 (≠ G220), E208 (= E222), W209 (= W223), L235 (= L249), L236 (≠ M250)
- binding : S9 (= S17), T43 (= T51), D44 (= D52), R47 (= R55), V145 (= V159), R163 (≠ Y177), Y168 (≠ I182), E172 (≠ S186), V177 (= V191), K180 (= K194), S181 (≠ A195), Y187 (= Y201), E207 (= E221), E208 (= E222), W209 (= W223), V211 (≠ A225), R237 (= R251), K241 (= K255), L272 (= L286), M273 (≠ F287), G274 (≠ F288), E282 (= E296), S299 (= S313), P303 (≠ A317), V304 (≠ I318), K309 (= K323), W312 (= W326), R319 (= R333), P357 (≠ T364), R358 (= R365), R417 (= R428), Q432 (≠ S443), R435 (≠ F446), L442 (≠ S453), E443 (≠ R454), T444 (≠ S455), G446 (≠ P457), L447 (= L458), F448 (= F459)
2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 2cv1A
- active site: K246 (= K260)
- binding adenosine-5'-triphosphate: P8 (= P16), S9 (= S17), G17 (= G25), T18 (= T26), I21 (= I29), R47 (= R55), A206 (≠ G220), W209 (= W223), L235 (= L249), L236 (≠ M250)
- binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R13), A7 (= A15), E41 (= E49), Y187 (= Y201), R205 (= R219), W209 (= W223)
- binding : S9 (= S17), E41 (= E49), T43 (= T51), D44 (= D52), R47 (= R55), V145 (= V159), R163 (≠ Y177), V166 (= V180), E172 (≠ S186), V177 (= V191), K180 (= K194), S181 (≠ A195), Y187 (= Y201), E207 (= E221), E208 (= E222), W209 (= W223), V211 (≠ A225), R237 (= R251), K241 (= K255), K243 (= K257), M273 (≠ F287), G274 (≠ F288), S276 (≠ Q290), E282 (= E296), S299 (= S313), P303 (≠ A317), V304 (≠ I318), K309 (= K323), W312 (= W326), R319 (= R333), P357 (≠ T364), R358 (= R365), R417 (= R428), L427 (= L438), Q432 (≠ S443), R435 (≠ F446), L442 (≠ S453), E443 (≠ R454), T444 (≠ S455), G446 (≠ P457), L447 (= L458), F448 (= F459)
2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 2cuzA
1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 1n78A
- active site: K246 (= K260)
- binding glutamol-amp: R5 (= R13), A7 (= A15), P8 (= P16), S9 (= S17), G17 (= G25), T18 (= T26), I21 (= I29), E41 (= E49), Y187 (= Y201), N191 (= N205), R205 (= R219), A206 (≠ G220), E208 (= E222), W209 (= W223), L235 (= L249), L236 (≠ M250)
- binding : S9 (= S17), T43 (= T51), D44 (= D52), R47 (= R55), V145 (= V159), R163 (≠ Y177), V166 (= V180), Y168 (≠ I182), E172 (≠ S186), V177 (= V191), K180 (= K194), S181 (≠ A195), Y187 (= Y201), E207 (= E221), E208 (= E222), W209 (= W223), L210 (= L224), V211 (≠ A225), R237 (= R251), K241 (= K255), M273 (≠ F287), G274 (≠ F288), E282 (= E296), R297 (≠ N311), P303 (≠ A317), V304 (≠ I318), K309 (= K323), W312 (= W326), R319 (= R333), P357 (≠ T364), R358 (= R365), R417 (= R428), L427 (= L438), Q432 (≠ S443), R435 (≠ F446), L442 (≠ S453), E443 (≠ R454), T444 (≠ S455), G446 (≠ P457), L447 (= L458), F448 (= F459)
1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 1j09A
- active site: K246 (= K260)
- binding adenosine-5'-triphosphate: H15 (= H23), E208 (= E222), L235 (= L249), L236 (≠ M250), K243 (= K257), I244 (≠ L258), S245 (= S259), K246 (= K260), R247 (= R261)
- binding glutamic acid: R5 (= R13), A7 (= A15), S9 (= S17), E41 (= E49), Y187 (= Y201), N191 (= N205), R205 (= R219), W209 (= W223)
P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of P27000
- R358 (= R365) mutation to Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).
1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
45% identity, 96% coverage: 10:477/488 of query aligns to 2:466/468 of 1g59A
- binding : D44 (= D52), R45 (≠ A53), A46 (≠ T54), R47 (= R55), P109 (= P117), V145 (= V159), R163 (≠ Y177), V166 (= V180), E172 (≠ S186), V177 (= V191), K180 (= K194), S181 (≠ A195), D182 (= D196), E207 (= E221), E208 (= E222), R237 (= R251), K241 (= K255), T242 (≠ S256), K243 (= K257), M273 (≠ F287), G274 (≠ F288), E282 (= E296), S299 (= S313), L300 (≠ K314), P303 (≠ A317), V304 (≠ I318), K309 (= K323), W312 (= W326), R319 (= R333), P357 (≠ T364), R358 (= R365), R417 (= R428), K426 (= K437), L427 (= L438), Q432 (≠ S443), R435 (≠ F446), L442 (≠ S453), E443 (≠ R454), T444 (≠ S455), P445 (≠ L456), G446 (≠ P457), L447 (= L458), F448 (= F459)
4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
39% identity, 97% coverage: 9:479/488 of query aligns to 1:480/485 of 4griB
- active site: S9 (= S17), K253 (= K260)
- binding glutamic acid: R5 (= R13), A7 (= A15), S9 (= S17), E41 (= E49), Y194 (= Y201), R212 (= R219), W216 (= W223)
- binding zinc ion: C105 (= C113), C107 (= C115), Y128 (= Y136), C132 (= C140)
3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
35% identity, 96% coverage: 10:479/488 of query aligns to 103:560/564 of 3al0C
- active site: S110 (= S17), K335 (= K260)
- binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R13), A108 (= A15), P109 (= P16), G118 (= G25), T122 (≠ I29), E142 (= E49), Y276 (= Y201), R294 (= R219), G295 (= G220), D297 (≠ E222), H298 (≠ W223), L324 (= L249), I325 (≠ M250), L333 (= L258)
- binding : T144 (= T51), D145 (= D52), R148 (= R55), Y208 (≠ P117), P213 (≠ Q122), K252 (≠ Y177), M255 (≠ V180), I266 (≠ V191), K269 (= K194), S270 (≠ A195), Y276 (= Y201), D297 (≠ E222), H298 (≠ W223), L299 (= L224), S300 (≠ A225), N301 (≠ S226), K304 (= K229), R330 (≠ K255), P332 (≠ K257), G363 (≠ F288), W364 (≠ I289), R365 (≠ Q290), E370 (= E296), S387 (= S313), K389 (≠ A315), V391 (≠ A317), I392 (= I318), K397 (= K323), W400 (= W326), R407 (= R333), E446 (≠ T364), K447 (≠ R365), Q453 (≠ E371), I457 (≠ L375), R509 (= R428), K520 (= K439), Q524 (≠ S443), R527 (≠ F446), V535 (≠ R454), T536 (≠ S455), G538 (≠ P457), L539 (= L458)
6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
35% identity, 97% coverage: 10:481/488 of query aligns to 3:501/502 of 6brlA
Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
45% identity, 69% coverage: 10:346/488 of query aligns to 3:337/485 of Q8DLI5
- R6 (= R13) binding L-glutamate
- Y192 (= Y201) binding L-glutamate
2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
45% identity, 69% coverage: 10:346/488 of query aligns to 2:336/484 of 2cfoA
P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
34% identity, 97% coverage: 10:482/488 of query aligns to 3:465/471 of P04805
- C98 (= C113) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
- C100 (= C115) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
- C125 (= C140) mutation to S: Loss of activity. Strong decrease in zinc content.
- H127 (≠ N142) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
- H129 (≠ T144) mutation to Q: No change in activity or in zinc content.
- H131 (≠ E146) mutation to Q: No change in activity or in zinc content.
- H132 (≠ E147) mutation to Q: No change in activity or in zinc content.
- C138 (≠ T158) mutation to S: No change in activity or in zinc content.
- S239 (= S259) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.
8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
34% identity, 97% coverage: 10:482/488 of query aligns to 3:465/468 of 8i9iA
4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
34% identity, 67% coverage: 10:334/488 of query aligns to 3:298/380 of 4g6zA
3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
28% identity, 60% coverage: 3:293/488 of query aligns to 4:283/455 of 3aiiA
P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
29% identity, 52% coverage: 13:267/488 of query aligns to 19:248/308 of P27305
- E55 (= E49) binding L-glutamate
- Y182 (= Y201) binding L-glutamate
- R200 (= R219) binding L-glutamate
4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
30% identity, 51% coverage: 13:261/488 of query aligns to 7:230/290 of 4a91A
- active site: S11 (= S17), K229 (= K260)
- binding glutamic acid: R7 (= R13), A9 (= A15), S11 (= S17), E43 (= E49), Y170 (= Y201), R188 (= R219), L192 (≠ W223)
- binding zinc ion: C99 (= C113), C101 (= C115), Y113 (= Y136), C117 (= C140)
O13775 Probable glutamate--tRNA ligase, cytoplasmic; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
26% identity, 52% coverage: 10:262/488 of query aligns to 207:446/716 of O13775
Sites not aligning to the query:
- 190 modified: Phosphoserine
Query Sequence
>WP_012759040.1 NCBI__GCF_000023185.1:WP_012759040.1
MTVSGTATGVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEF
ETKVLDALKWCGLEWKEGPDIGGPYGPYRQSDRKEMYQPYGQELLDKGHAFRCFCTPARL
EQMRETQRAAGKPPKYDGLCLNLTAEEVTSRIAAGETTVIRMKIPAEGSCDFTDGVYGDV
SIPWDSVDMQVLVKADGMPTYHMANVIDDHLMKITHVARGEEWLASVPKHILLYRYFDWD
QPVFMHLSLMRNADKSKLSKRKNPTSISYYSALGYIPEALMNFLGLFFIQIAEGEELLGM
DELSEKFDPANLSKAGAIFDIQKLDWLNGRWIREKLSEEEFQARVLTWAMENDRLKEGLR
LSQTRISKLGELPDLAGFLLKSDLGLQPSDFAKIKSPPEEILEILNTVQPDLEKILEWNV
ETIEAELRAIADRMGKKLKVVVSPLFVAVSGSSRSLPLFDSMAILGRSVVRQRLKLASQA
VAALVGSK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory