SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012759162.1 NCBI__GCF_000023185.1:WP_012759162.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
33% identity, 92% coverage: 12:281/293 of query aligns to 6:265/485 of Q8DLI5

query
sites
Q8DLI5

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R6 (= R12) binding L-glutamate
Y192 (= Y208) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
33% identity, 92% coverage: 12:281/293 of query aligns to 5:264/484 of 2cfoA

query
sites
2cfoA

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active site: S9 (= S16), L239 (= L256), K250 (= K267)
binding glutamic acid: R5 (= R12), Y191 (= Y208), R209 (= R226), H213 (≠ L230)

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 1g59A

query
sites
1g59A

V

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binding : D44 (= D51), R45 (≠ Q52), A46 (≠ T53), R47 (= R54), P109 (≠ R108), V145 (≠ A158), R163 (≠ T184), V166 (≠ I187), E172 (≠ V193), V177 (≠ I198), K180 (≠ R201), S181 (= S202), D182 (= D203), E207 (≠ L228), E208 (≠ D229), R237 (≠ L258), K241 (≠ G262), T242 (≠ R263), K243 (= K264)

Sites not aligning to the query:

binding : 273, 274, 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 2cv2A

query
sites
2cv2A

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active site: K246 (= K267)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R12), A7 (= A14), S9 (= S16), G17 (= G24), I21 (≠ S28), E41 (= E48), Y187 (= Y208), R205 (= R226), A206 (≠ G227), E208 (≠ D229), W209 (≠ L230), L235 (= L256), L236 (≠ I257)
binding : S9 (= S16), T43 (≠ I50), D44 (= D51), R47 (= R54), V145 (≠ A158), R163 (≠ T184), Y168 (≠ A189), E172 (≠ V193), V177 (≠ I198), K180 (≠ R201), S181 (= S202), Y187 (= Y208), E207 (≠ L228), E208 (≠ D229), W209 (≠ L230), V211 (≠ H232), R237 (≠ L258), K241 (≠ G262), L272 (= L293)

Sites not aligning to the query:

binding : 273, 274, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448

2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 2cv1A

query
sites
2cv1A

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M

F

N

N

R

Y

D

A

M

W

A

A

E

R

A

R

E

N

Q

G

A

G

R

R

L

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

Q

R

T

A

R
|
R

C

Y

T

V

P

P

E

G

F

A

E

E

A

E

G

R

I

I

L

L

R

A

D

A

L

L

E

K

W

W

L

L

-

G

-

L

-

S

-

Y

-

D

-

E

-

G

-

P

D

D

I

V

G

G

W

G

A

P

S

H

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

P

E

L

Y

Y

Q

Q

A

K

A

Y

L

A

H

E

A

E

L

L

I

L

E

K

R

R

G

G

L

W

V

A

Y

Y

P

R

A

A

F

F

L

E

T

T

R

P

G

E

E

E

V

L

K

E

A

Q

R

-

I

-

A

I

A

R

Y

K

E

E

A

K

A

G

G

G

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

D

A

G

S

R

D

A

R

R

E

N

R

I

P

P

A

P

D

E

E

E

R

A

R

E

D

E

M

R

L

A

T

R

S

R

G

G

K

E

K

P

H

H

A
x
V

W

I

R

R

L

L

D

K

M

V

R

P

K

R

A

P

L

G

D

T

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

T

E

E

T

V

G

K

D

D

G

E

R

L

T
x
R

G

G

E

V

I
x
V

A

V

A

Y

E

D

P

N

D

Q

V
x
E

W

I

G

P

D

D

V

V

I
x
V

L

L

S

L

R
x
K

S
|
S

D

D

A

G

P

Y

S

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G
x
A

L
x
E

D
x
E

L
x
W

F

L

H
x
V

A

S

T

T

S

P

V

I

H

H

R

V

L

L

Q

Y

V

R

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

R

Y

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

L
x
R

G

N

A

P

D

D

G
x
K

R

T

K
|
K

L

I

S

S

K
|
K

S

R

Q

K

G

S

D

H

S

T

G

S

L

L

G

D

E

W

L

Y

R

K

A

A

Q

E

G

G

M

F

S

L

G

P

A

E

D

A

I

L

R

R

R

N

L

Y

V

L

G

C

L

L

active site: K246 (= K267)
binding adenosine-5'-triphosphate: P8 (= P15), S9 (= S16), G17 (= G24), T18 (≠ H25), I21 (≠ S28), R47 (= R54), A206 (≠ G227), W209 (≠ L230), L235 (= L256), L236 (≠ I257)
binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R12), A7 (= A14), E41 (= E48), Y187 (= Y208), R205 (= R226), W209 (≠ L230)
binding : S9 (= S16), E41 (= E48), T43 (≠ I50), D44 (= D51), R47 (= R54), V145 (≠ A158), R163 (≠ T184), V166 (≠ I187), E172 (≠ V193), V177 (≠ I198), K180 (≠ R201), S181 (= S202), Y187 (= Y208), E207 (≠ L228), E208 (≠ D229), W209 (≠ L230), V211 (≠ H232), R237 (≠ L258), K241 (≠ G262), K243 (= K264)

Sites not aligning to the query:

binding : 273, 274, 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 2cuzA

query
sites
2cuzA

V

V

F

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

N

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

L

Y

S

I

A

A

L

L

M

F

N

N

R

Y

D

A

M

W

A

A

E

R

A

R

E

N

Q

G

A

G

R

R

L

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

Q

R

T

A

R

R

C

Y

T

V

P

P

E

G

F

A

E

E

A

E

G

R

I

I

L

L

R

A

D

A

L

L

E

K

W

W

L

L

-

G

-

L

-

S

-

Y

-

D

-

E

-

G

-

P

D

D

I

V

G

G

W

G

A

P

S

H

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

P

E

L

Y

Y

Q

Q

A

K

A

Y

L

A

H

E

A

E

L

L

I

L

E

K

R

R

G

G

L

W

V

A

Y

Y

P

R

A

A

F

F

L

E

T

T

R

P

G

E

E

E

V

L

K

E

A

Q

R

-

I

-

A

I

A

R

Y

K

E

E

A

K

A

G

G

G

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

D

A

G

S

R

D

A

R

R

E

N

R

I

P

P

A

P

D

E

E

E

R

A

R

E

D

E

M

R

L

A

T

R

S

R

G

G

K

E

K

P

H

H

A

V

W

I

R

R

L

L

D

K

M

V

R

P

K

R

A

P

L

G

D

T

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

T

E

E

T

V

G

K

D

D

G

E

R

L

T

R

G

G

E

V

I

V

A

V

A

Y

E

D

P

N

D

Q

V

E

W

I

G

P

D

D

V

V

I

V

L

L

S

L

R

K

S

S

D

D

A

G

P

Y

S

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G

A

L

E

D

E

L

W

F

L

H

V

A

S

T

T

S

P

V

I

H

H

R

V

L

L

Q

Y

V

R

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

R

Y

F

H

Y

H

H

H

M

R

P

L

L

I

L

L

R

G

N

A

P

D

D

G

K

R

T

K

K

L

I

S

S

K
|
K

S

R

Q

K

G

S

D

H

S

T

G

S

L

L

G

D

E

W

L

Y

R

K

A

A

Q

E

G

G

M

F

S

L

G

P

A

E

D

A

I

L

R

R

R

N

L

Y

V

L

G

C

L

L

active site: K246 (= K267)
binding glutamic acid: R5 (= R12), A7 (= A14), S9 (= S16), E41 (= E48), Y187 (= Y208), R205 (= R226)

1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 1n78A

query
sites
1n78A

V

V

F

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

N

T

G

G

P

D

L

P

H

H

L

V

G
|
G

H
x
T

A

A

L

Y

S
x
I

A

A

L

L

M

F

N

N

R

Y

D

A

M

W

A

A

E

R

A

R

E

N

Q

G

A

G

R

R

L

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

Q

R

T

A

R
|
R

C

Y

T

V

P

P

E

G

F

A

E

E

A

E

G

R

I

I

L

L

R

A

D

A

L

L

E

K

W

W

L

L

-

G

-

L

-

S

-

Y

-

D

-

E

-

G

-

P

D

D

I

V

G

G

W

G

A

P

S

H

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

P

E

L

Y

Y

Q

Q

A

K

A

Y

L

A

H

E

A

E

L

L

I

L

E

K

R

R

G

G

L

W

V

A

Y

Y

P

R

A

A

F

F

L

E

T

T

R

P

G

E

E

E

V

L

K

E

A

Q

R

-

I

-

A

I

A

R

Y

K

E

E

A

K

A

G

G

G

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

D

A

G

S

R

D

A

R

R

E

N

R

I

P

P

A

P

D

E

E

E

R

A

R

E

D

E

M

R

L

A

T

R

S

R

G

G

K

E

K

P

H

H

A
x
V

W

I

R

R

L

L

D

K

M

V

R

P

K

R

A

P

L

G

D

T

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

T

E

E

T

V

G

K

D

D

G

E

R

L

T
x
R

G

G

E

V

I
x
V

A

V

A
x
Y

E

D

P

N

D

Q

V
x
E

W

I

G

P

D

D

V

V

I
x
V

L

L

S

L

R
x
K

S
|
S

D

D

A

G

P

Y

S

P

S

T

Y
|
Y

H

H

L

L

S

A

V
x
N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G
x
A

L
x
E

D
x
E

L
x
W

F
x
L

H
x
V

A

S

T

T

S

P

V

I

H

H

R

V

L

L

Q

Y

V

R

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

R

Y

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

L
x
R

G

N

A

P

D

D

G
x
K

R

T

K

K

L

I

S

S

K
|
K

S

R

Q

K

G

S

D

H

S

T

G

S

L

L

G

D

E

W

L

Y

R

K

A

A

Q

E

G

G

M

F

S

L

G

P

A

E

D

A

I

L

R

R

R

N

L

Y

V

L

G

C

L

L

active site: K246 (= K267)
binding glutamol-amp: R5 (= R12), A7 (= A14), P8 (= P15), S9 (= S16), G17 (= G24), T18 (≠ H25), I21 (≠ S28), E41 (= E48), Y187 (= Y208), N191 (≠ V212), R205 (= R226), A206 (≠ G227), E208 (≠ D229), W209 (≠ L230), L235 (= L256), L236 (≠ I257)
binding : S9 (= S16), T43 (≠ I50), D44 (= D51), R47 (= R54), V145 (≠ A158), R163 (≠ T184), V166 (≠ I187), Y168 (≠ A189), E172 (≠ V193), V177 (≠ I198), K180 (≠ R201), S181 (= S202), Y187 (= Y208), E207 (≠ L228), E208 (≠ D229), W209 (≠ L230), L210 (≠ F231), V211 (≠ H232), R237 (≠ L258), K241 (≠ G262)

Sites not aligning to the query:

binding : 273, 274, 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of 1j09A

query
sites
1j09A

V

V

F

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

N

T

G

G

P

D

L

P

H
|
H

L

V

G

G

H

T

A

A

L

Y

S

I

A

A

L

L

M

F

N

N

R

Y

D

A

M

W

A

A

E

R

A

R

E

N

Q

G

A

G

R

R

L

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

Q

R

T

A

R

R

C

Y

T

V

P

P

E

G

F

A

E

E

A

E

G

R

I

I

L

L

R

A

D

A

L

L

E

K

W

W

L

L

-

G

-

L

-

S

-

Y

-

D

-

E

-

G

-

P

D

D

I

V

G

G

W

G

A

P

S

H

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

P

E

L

Y

Y

Q

Q

A

K

A

Y

L

A

H

E

A

E

L

L

I

L

E

K

R

R

G

G

L

W

V

A

Y

Y

P

R

A

A

F

F

L

E

T

T

R

P

G

E

E

E

V

L

K

E

A

Q

R

-

I

-

A

I

A

R

Y

K

E

E

A

K

A

G

G

G

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

D

A

G

S

R

D

A

R

R

E

N

R

I

P

P

A

P

D

E

E

E

R

A

R

E

D

E

M

R

L

A

T

R

S

R

G

G

K

E

K

P

H

H

A

V

W

I

R

R

L

L

D

K

M

V

R

P

K

R

A

P

L

G

D

T

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

T

E

E

T

V

G

K

D

D

G

E

R

L

T

R

G

G

E

V

I

V

A

V

A

Y

E

D

P

N

D

Q

V

E

W

I

G

P

D

D

V

V

I

V

L

L

S

L

R

K

S

S

D

D

A

G

P

Y

S

P

S

T

Y
|
Y

H

H

L

L

S

A

V
x
N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R
|
R

G

A

L

E

D
x
E

L
x
W

F

L

H

V

A

S

T

T

S

P

V

I

H

H

R

V

L

L

Q

Y

V

R

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

R

Y

F

H

Y

H

H

H

M

R

P

L
|
L

I
x
L

L

R

G

N

A

P

D

D

G

K

R

T

K
|
K

L
x
I

S
|
S

K
|
K

S
x
R

Q

K

G

S

D

H

S

T

G

S

L

L

G

D

E

W

L

Y

R

K

A

A

Q

E

G

G

M

F

S

L

G

P

A

E

D

A

I

L

R

R

R

N

L

Y

V

L

G

C

L

L

active site: K246 (= K267)
binding adenosine-5'-triphosphate: H15 (= H22), E208 (≠ D229), L235 (= L256), L236 (≠ I257), K243 (= K264), I244 (≠ L265), S245 (= S266), K246 (= K267), R247 (≠ S268)
binding glutamic acid: R5 (= R12), A7 (= A14), S9 (= S16), E41 (= E48), Y187 (= Y208), N191 (≠ V212), R205 (= R226), W209 (≠ L230)

P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 97% coverage: 10:293/293 of query aligns to 3:272/468 of P27000

query
sites
P27000

V

V

F

T

R

R

F

I

A

A

P

P

S

S

P

P

N

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

L

Y

S

I

A

A

L

L

M

F

N

N

R

Y

D

A

M

W

A

A

E

R

A

R

E

N

Q

G

A

G

R

R

L

F

L

I

L

V

R

R

I

I

E

E

D

D

I

T

D

D

Q

R

T

A

R

R

C

Y

T

V

P

P

E

G

F

A

E

E

A

E

G

R

I

I

L

L

R

A

D

A

L

L

E

K

W

W

L

L

-

G

-

L

-

S

-

Y

-

D

-

E

-

G

-

P

D

D

I

V

G

G

W

G

A

P

S

H

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

P

E

L

Y

Y

Q

Q

A

K

A

Y

L

A

H

E

A

E

L

L

I

L

E

K

R

R

G

G

L

W

V

A

Y

Y

P

R

A

A

F

F

L

E

T

T

R

P

G

E

E

E

V

L

K

E

A

Q

R

-

I

-

A

I

A

R

Y

K

E

E

A

K

A

G

G

G

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

D

A

G

S

R

D

A

R

R

E

N

R

I

P

P

A

P

D

E

E

E

R

A

R

E

D

E

M

R

L

A

T

R

S

R

G

G

K

E

K

P

H

H

A

V

W

I

R

R

L

L

D

K

M

V

R

P

K

R

A

P

L

G

D

T

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

T

E

E

T

V

G

K

D

D

G

E

R

L

T

R

G

G

E

V

I

V

A

V

A

Y

E

D

P

N

D

Q

V

E

W

I

G

P

D

D

V

V

I

V

L

L

S

L

R

K

S

S

D

D

A

G

P

Y

S

P

S

T

Y

Y

H

H

L

L

S

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

V

T

T

H

D

V

V

V

I

R

R

G

A

L

E

D

E

L

W

F

L

H

V

A

S

T

T

S

P

V

I

H

H

R

V

L

L

Q

Y

V

R

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

R

Y

F

H

Y

H

H

H

M

R

P

L

L

I

L

L

R

G

N

A

P

D

D

G

K

R

T

K

K

L

I

S

S

K

K

S

R

Q

K

G

S

D

H

S

T

G

S

L

L

G

D

E

W

L

Y

R

K

A

A

Q

E

G

G

M

F

S

L

G

P

A

E

D

A

I

L

R

R

R

N

L

Y

V

L

G

C

L

L

Sites not aligning to the query:

358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).

P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
32% identity, 92% coverage: 12:281/293 of query aligns to 6:254/471 of P04805

query
sites
P04805

R

R

F

F

A

A

P

P

S

S

P

P

N

T

G

G

P

Y

L

L

H

H

L

V

G

G

H

G

A

A

L

R

S

T

A

A

L

L

M

Y

N

S

R

W

D

L

M

F

A

A

E

R

A

N

E

H

Q

G

A

G

R

E

L

F

L

V

L

L

R

R

I

I

E

E

D

D

I

T

D

D

Q

L

T

E

R

R

C

S

T

T

P

P

E

E

F

A

E

I

A

E

G

A

I

I

L

M

R

D

D

G

L

M

E

N

W

W

L

L

D

S

I

L

G

E

W

W

A

D

S

E

P

G

V

P

R

Y

Q

Q

S

T

E

K

H

R

I

F

P

D

E

R

Y

Y

Q

N

A

A

A

V

L

I

H

D

A

Q

L

M

I

L

E

E

R

E

G

G

L

T

V

A

Y

Y

P

K

A
x
C

F

Y

L
x
C

T

S

R

K

G

E

E

R

V

L

K

E

A

A

R

L

I

R

A

E

Y

Q

E

M

A

A

A

K

G

G

E

E

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

K

P

P

H

R

Y

Y

P

D

A

G

S

R

D
x
C

R

R

-
x
H

-

S

-
x
H

E

E

R
x
H

P
x
H

A

A

D

D

E

D

R

E

R

P

D
x
C

M

V

L

V

T

-

S

-

G

-

K

-

H

-

A

-

W

-

R

-

L

-

D

-

M

-

R

R

K

F

A

A

L

N

D

P

L

Q

I

E

G

G

E

S

L

V

F

F

W

-

T

-

E

-

T

-

G

D

D

D

G

Q

R

I

T

R

G

G

E

P

I

I

A

E

A

F

E

S

P

N

D

Q

V

E

W

L

G

D

D

D

V

L

I

I

L

I

S

R

R

R

S

T

D

D

A

G

P

S

S

P

S

T

Y

Y

H

N

L

F

S

C

V

V

D

D

A

W

L

D

Q

M

G

E

V

I

T

T

H

H

V

V

V

I

R

R

G

G

L

E

D

D

L

-

F

-

H

H

A

I

T

N

S

N

V

T

H

P

R

R

L

Q

L

I

Q

N

V

I

L

L

L

K

G

A

L

L

-

K

-

A

P

P

Q

V

P

P

V

V

Y

Y

H

A

H

H

H

V

R

S

L

M

I

I

L

N

G

G

A

D

D

D

G

G

R

K

K

K

L

L

S
|
S

K

K

S

R

Q

H

G

G

D

A

S

V

G

S

L

V

G

M

E

Q

L

Y

R

R

A

D

Q

D

G

G

C98 (≠ A104) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (≠ L106) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (≠ D139) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (vs. gap) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (vs. gap) mutation to Q: No change in activity or in zinc content.
H131 (≠ R142) mutation to Q: No change in activity or in zinc content.
H132 (≠ P143) mutation to Q: No change in activity or in zinc content.
C138 (≠ D149) mutation to S: No change in activity or in zinc content.
S239 (= S266) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
32% identity, 92% coverage: 12:281/293 of query aligns to 6:254/468 of 8i9iA

query
sites
8i9iA

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

N

T

G

G

P

Y

L

L

H

H

L

V

G

G

H

G

A

A

L

R

S

T

A

A

L

L

M

Y

N

S

R

W

D

L

M

F

A

A

E

R

A

N

E

H

Q

G

A

G

R

E

L

F

L

V

L

L

R

R

I

I

E

E

D

D

I

T

D

D

Q

L

T

E

R

R

C

S

T

T

P

P

E

E

F

A

E

I

A

E

G

A

I

I

L

M

R

D

D

G

L

M

E

N

W

W

L

L

D

S

I

L

G

E

W

W

A

D

S

E

P

G

V

P

R

Y

Q

Q

S

T

E

K

H

R

I

F

P

D

E

R

Y

Y

Q

N

A

A

A

V

L

I

H

D

A

Q

L

M

I

L

E

E

R

E

G

G

L

T

V

A

Y

Y

P

K

A

C

F

Y

L
x
C

T

S

R

K

G

E

E

R

V

L

K

E

A

A

R

L

I

R

A

E

Y

Q

E

M

A

A

A

K

G

G

E

E

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

K

P

P

H

R

Y
|
Y

P

D

A

G

S

R

D
x
C

R

R

-

H

-

S

-

H

E

E

R

H

P

H

A

A

D

D

E

D

R

E

R

P

D

C

M

V

L

V

T

-

S

-

G

-

K

-

H

-

A

-

W

-

R

-

L

-

D

-

M

-

R

R

K

F

A

A

L

N

D

P

L

Q

I

E

G

G

E

S

L

V

F

F

W

-

T

-

E

-

T

-

G

D

D

D

G

Q

R

I

T

R

G

G

E

P

I

I

A

E

A

F

E

S

P

N

D

Q

V

E

W

L

G

D

D

D

V

L

I

I

L

I

S

R

R

R

S

T

D

D

A

G

P

S

S

P

S

T

Y

Y

H

N

L

F

S

C

V

V

D

D

A

W

L

D

Q

M

G

E

V

I

T

T

H

H

V

V

V

I

R
|
R

G

G

L

E

D

D

L

-

F

-

H
|
H

A

I

T

N

S

N

V

T

H

P

R

R

L

Q

L

I

Q

N

V

I

L

L

L

K

G

A

L

L

-

K

-

A

P

P

Q

V

P

P

V

V

Y

Y

H

A

H

H

H

V

R

S

L

M

I

I

L

N

G

G

A

D

D

D

G

G

R

E

K

K

L

L

S

S

K

K

S

R

Q

H

G

G

D

A

S

V

G

S

L

V

G

M

E

Q

L

Y

R

R

A

D

Q

D

G

G

binding glutamic acid: R6 (= R12), A8 (= A14), P9 (= P15), S10 (= S16), R199 (= R226), H203 (= H232)
binding zinc ion: C100 (≠ L106), Y121 (= Y135), C125 (≠ D139)

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
31% identity, 96% coverage: 12:293/293 of query aligns to 5:279/485 of 4griB

query
sites
4griB

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

N

T

G

G

P

L

L

Q

H

H

L

I

G

G

H

G

A

I

L

R

S

T

A

A

L

L

M

F

N

N

R

Y

D

F

M

F

A

A

E

K

A

S

E

C

Q

G

A

G

R

K

L

F

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

Q

Q

T

S

R

R

C

Y

T

S

P

P

E

E

F

A

E

E

A

N

G

D

I

L

L

Y

R

S

D

S

L

L

E

K

W

W

L

L

D

G

I

I

G

S

W

F

-

D

A

E

S

G

P

P

V

V

-

V

-

G

-

D

-

Y

-

A

-

P

R

Y

R

V

Q

Q

S

S

E

Q

H

R

I

S

P

A

E

I

Y

Y

Q

K

A

Q

A

Y

L

A

H

K

A

Y

L

L

I

I

E

E

R

S

G

G

L

H

V

A

Y

Y

P

Y

A
x
C

F

Y

L
x
C

T

S

R

-

G

P

E

E

V

R

K

L

A

E

R

R

I

I

A

K

Y

I

E

Q

A

N

A

I

G

N

E

K

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

M

T

P

P

P

H

G

Y
|
Y

P

D

A

R

S

H

D
x
C

R

R

E

N

R

L

P

S

A

N

D

E

E

E

R

V

R

E

D

N

M

A

L

L

T

I

S

K

G

K

K

I

K

K

H

P

A

V

W

V

R

R

L

F

D

-

M

-

R

-

K

-

A

K

L

I

D

P

L

L

I

E

G

G

E

D

L

-

F

-

W

-

T

T

E

S

T

F

G

D

D

D

G

I

R

L

T

L

G

G

E

R

I

I

A

T

-

W

A

A

E

N

P

K

D

D

V

I

W

S

G

P

D

D

V

P

I

V

L

I

S

L

R

K

S

S

D

D

A

G

P

L

S

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

V

V

D

D

A

Y

L

L

Q

M

G

K

V

I

T

T

H

H

V

V

V

L

R
|
R

G

A

L

Q

D

E

L
x
W

F

V

H

S

A

S

T

G

S

P

V

L

H

H

R

V

L

L

Q

Y

V

K

L

A

L

F

G

K

L

W

P

K

Q

P

P

P

V

I

Y

Y

H

C

H

H

H

L

R

P

L

M

I

V

L

M

G

G

A

N

D

D

G

G

R

Q

K

K

L

L

S

S

K
|
K

S

R

Q

H

G

G

D

S

S

T

G

A

L

L

G

R

E

Q

L

F

R

I

A

E

Q

D

G

G

M

Y

S

L

G

P

A

E

D

A

I

I

R

I

R

N

L

Y

V

V

G

T

L

L

active site: S9 (= S16), K253 (= K267)
binding glutamic acid: R5 (= R12), A7 (= A14), S9 (= S16), E41 (= E48), Y194 (= Y208), R212 (= R226), W216 (≠ L230)
binding zinc ion: C105 (≠ A104), C107 (≠ L106), Y128 (= Y135), C132 (≠ D139)

4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
35% identity, 88% coverage: 12:268/293 of query aligns to 7:230/290 of 4a91A

query
sites
4a91A

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

N

S

G

G

P

E

L

L

H

H

L

F

G

G

H

S

A

L

L

I

S

A

A

A

L

L

M

G

N

S

R

Y

D

L

M

Q

A

A

E

R

A

A

E

R

Q

Q

A

G

R

R

L

W

L

L

L

V

R

R

I

I

E
|
E

D

D

I

I

D

D

Q

P

T

P

R

R

C

E

T

V

P

P

E

G

F

A

E

A

A

E

G

T

I

I

L

L

R

R

D

Q

L

L

E

E

W

H

L

Y

D

G

I

L

G

H

W

W

A

D

S

G

P

D

V

V

R

L

R

W

Q

Q

S

S

E

Q

H

R

I

H

P

D

E

A

Y

Y

Q

R

A

E

A

A

L

L

H

A

A

W

L

L

I

H

E

E

R

Q

G

G

L

L

V

S

Y

Y

P

Y

A
x
C

F

T

L
x
C

T

T

R

R

G

A

E

R

V

I

K

Q

A

S

R

I

I

G

A

G

A

I

Y
|
Y

E

D

A

G

A

H

G
x
C

E

R

P

V

W

L

P

H

H

H

D

G

P

P

D

D

G

N

T

A

P

A

H

-

Y

-

P

V

A

R

S

I

D

R

R

Q

E

Q

R

H

P

P

A

V

D

T

E

Q

R

F

R

T

D

D

M

Q

L

L

T

-

S

R

G

G

K

I

H

H

A

A

W

-

R

-

L

-

D

D

M

E

R

K

K

L

A

A

L

R

D

E

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

-

E

-

T

-

G

-

D

-

G

-

R

-

T

-

G

-

E

-

I

-

A

-

E

-

P

-

D

-

V

-

W

-

G

-

D

D

V

F

I

I

L

I

S

H

R

R

S

R

D

D

A

G

P

L

S

F

S

A

Y
|
Y

H

N

L

L

S

A

V

V

D

D

A

H

L

F

Q

Q

G

G

V

V

T

T

H

E

V

I

V

V

R
|
R

G

G

L

A

D

D

L
|
L

F

I

H

E

A

P

T

T

S

V

V

R

H

Q

R

I

L

S

L

L

Q

Y

V

Q

L

L

L

F

G

G

L

W

P

K

Q

V

P

P

V

D

Y

Y

H

I

H

H

H

L

R

P

L

L

I

A

L

L

G

N

A

P

D

Q

G

G

R

A

K

K

L

L

S

S

K
|
K

S

Q

active site: S11 (= S16), K229 (= K267)
binding glutamic acid: R7 (= R12), A9 (= A14), S11 (= S16), E43 (= E48), Y170 (= Y208), R188 (= R226), L192 (= L230)
binding zinc ion: C99 (≠ A104), C101 (≠ L106), Y113 (= Y118), C117 (≠ G122)

P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
35% identity, 88% coverage: 12:269/293 of query aligns to 19:243/308 of P27305

query
sites
P27305

R

R

F

F

A

A

P

P

S

S

P

P

N

S

G

G

P

E

L

L

H

H

L

F

G

G

H

S

A

L

L

I

S

A

A

A

L

L

M

G

N

S

R

Y

D

L

M

Q

A

A

E

R

A

A

E

R

Q

Q

A

G

R

R

L

W

L

L

L

V

R

R

I

I

E
|
E

D

D

I

I

D

D

Q

P

T

P

R

R

C

E

T

V

P

P

E

G

F

A

E

A

A

E

G

T

I

I

L

L

R

R

D

Q

L

L

E

E

W

H

L

Y

D

G

I

L

G

H

W

W

A

D

S

G

P

D

V

V

R

L

R

W

Q

Q

S

S

E

Q

H

R

I

H

P

D

E

A

Y

Y

Q

R

A

E

A

A

L

L

H

A

A

W

L

L

I

H

E

E

R

Q

G

G

L

L

V

S

Y

Y

P

Y

A

C

F

T

L

C

T

T

R

R

G

A

E

R

V

I

K

Q

A

S

R

I

I

G

A

G

A

I

Y

Y

E

D

A

G

A

H

G

C

E

R

P

V

W

L

P

H

H

H

D

G

P

P

D

D

G

N

T

A

P

A

H

-

Y

-

P

V

A

R

S

I

D

R

R

Q

E

Q

R

H

P

P

A

V

D

T

E

Q

R

F

R

T

D

D

M

Q

L

L

T

-

S

R

G

G

K

I

H

H

A

A

W

-

R

-

L

-

D

D

M

E

R

K

K

L

A

A

L

R

D

E

L

-

I

-

G

-

E

-

L

-

F

-

W

-

T

-

E

-

T

-

G

-

D

-

G

-

R

-

T

-

G

-

E

-

I

-

A

-

E

-

P

-

D

-

V

-

W

-

G

-

D

D

V

F

I

I

L

I

S

H

R

R

S

R

D

D

A

G

P

L

S

F

S

A

Y
|
Y

H

N

L

L

S

A

V

V

D

D

A

H

L

F

Q

Q

G

G

V

V

T

T

H

E

V

I

V

V

R
|
R

G

G

L

A

D

D

L

L

F

I

H

E

A

P

T

T

S

V

V

R

H

Q

R

I

L

S

L

L

Q

Y

V

Q

L

L

L

F

G

G

L

W

P

K

Q

V

P

P

V

D

Y

Y

H

I

H

H

H

L

R

P

L

L

I

A

L

L

G

N

A

P

D

Q

G

G

R

A

K

K

L

L

S

S

K

K

S

Q

Q

N

E55 (= E48) binding L-glutamate
Y182 (= Y208) binding L-glutamate
R200 (= R226) binding L-glutamate

6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
31% identity, 91% coverage: 7:272/293 of query aligns to 1:266/502 of 6brlA

query
sites
6brlA

Q

E

K

K

P

V

V

R

F

V

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

N

T

G

G

P

P

L

L

H

H

L

L

G

G

H

G

A

V

L

R

S

T

A

A

L

L

M

Y

N

D

R

Y

D

L

M

F

A

A

E

K

A

H

E

N

Q

G

A

G

R

D

L

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

Q

T

T

Q

R

R

C

Y

T

V

P

P

E

G

F

S

E

E

A

E

G

Y

I

I

L

M

R

E

D

A

L

L

E

E

W

W

L

I

D

G

I

M

-

V

-

P

-

D

-

E

-

S

-

P

-

K

-

H

G

G

W

G

A

P

S

Y

P

A

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

R

P

D

E

I

Y

Y

Q

D

A

R

A

Y

L

T

H

E

A

Q

L

I

I

L

E

K

R

T

G

D

L

Y

V

A

Y

Y

P

L

A

A

F

F

L

D

T

T

R

P

G

E

E

E

V

L

K

D

A

Q

R

I

I

R

A

A

A

E

Y

F

E

E

A

A

A

R

G

G

E

D

P

V

W

F

P

A

H

Y

D

N

P

-

D

-

G

-

T

-

P

-

H

-

Y

Y

P

E

A

T

S

R

D

N

R

R

E

L

R

R

-

N

-

S

-

I

-

S

-

L

P

P

A

E

D

E

E

E

R

V

R

K

D

K

M

L

L

L

T

E

S

E

G

K

K

T

K

P

H

Y

A

V

W

I

R

R

L

F

D

K

M

M

R

-

K

-

A

P

L

L

D

D

L

R

I

I

G

I

E

N

L

L

L

-

F

-

W

-

T

-

E

-

T

-

G

N

D

D

G

I

R

I

T

R

G

G

E

K

I

F

A

S

A

V

E

N

P

T

D

N

V

T

W

L

G

D

D

D

V

K

I

V

L

L

S

V

R

K

S

N

D

D

A

G

P

M

S

P

S

T

Y
|
Y

H

H

L

F

S

A

V
x
N

V

I

D

D

A

H

L

E

Q

M

G

K

V

I

T

T

H

H

V

V

V

I

R
|
R

G

G

L

E

D

E

L
x
W

F

L

H

P

A

S

T

M

S

A

V

L

H

H

R

V

L

L

Q

Y

V

E

L

A

L

M

G

G

L

W

P

D

Q

A

P

P

V

E

Y

F

H

A

H

H

H

L

R

S

L

L

I

I

L

L

G

K

A

P

D

E

G

G

R

K

-

G

K

K

L

L

S

S

K
|
K

S

R

Q

D

G

G

D

D

S

K

active site: K261 (= K267)
binding glutamic acid: R6 (= R12), A8 (= A14), S10 (= S16), E42 (= E48), Y201 (= Y208), N205 (≠ V212), R219 (= R226), W223 (≠ L230)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
29% identity, 92% coverage: 12:282/293 of query aligns to 106:350/564 of 3al0C

query
sites
3al0C

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

N

T

G

G

P

H

L

L

H

H

L

V

G
|
G

H

G

A

A

L

R

S
x
T

A

A

L

L

M

F

N

N

R

W

D

M

M

F

A

A

E

R

A

K

E

E

Q

G

A

G

R

K

L

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

Q

T

T

E

R
|
R

C

S

T

S

P

R

E

E

F

Y

E

E

A

Q

G

Q

I

I

L

L

R

E

D

S

L

L

E

R

W

W

L

C

-

G

-

L

-

D

-

W

-

D

-

E

-

G

-

P

D

D

I

I

G

G

W

G

A

D

S

F

P

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

L

P

E

E

I

Y

Y

Q

R

A

E

A

Y

L

A

H

E

A

K

L

L

I

V

E

E

R

-

G

-

L

-

V

-

Y

-

P

-

A

-

F

-

L

-

T

-

R

-

G

-

E

-

V

-

K

-

A

-

R

-

I

-

A

-

Y

-

E

-

A

-

G

-

E

-

P

-

W

-

P

-

H

-

D

-

P

-

D

D

G

K

T

R

P

A

H

Y

Y

Y

P

V

A

V

S
x
Y

D

D

R

K

E

E

R

D

P
|
P

A

S

D

K

E

E

R

L

R

-

D

-

M

F

L

T

T

T

S

Y

G

E

K

Y

K

P

H

H

A

E

W

Y

R

K

L

E

D

K

M

G

R

H

K

P

A

V

L

T

D

I

L

K

I

F

G

K

E

V

L

L

L

P

F

G

W

K

T

T

E

S

T

F

G

E

D

D

G

L

R

L

T
x
K

G

G

E

Y

I
x
M

A

E

A

F

E

D

P

N

D

S

V

T

W

L

G

E

D

D

V

F

I
|
I

L

I

S

M

R
x
K

S
|
S

D

N

A

G

P

F

S

P

S

T

Y
|
Y

H

N

L

F

S

A

V

V

D

D

A

H

L

L

Q

M

G

R

V

I

T

S

H

H

V

V

V

F

R
|
R

G
|
G

L

E

D
|
D

L
x
H

F
x
L

H
x
S

A
x
N

T

T

S

P

V
x
K

H

Q

R

L

L

M

L

I

Q

Y

V

E

L

A

L

F

G

G

L

W

P

E

Q

A

P

P

V

V

Y

F

H

M

H

H

H

I

R

P

L
|
L

I
|
I

L

L

G

G

A

S

D

D

G
x
R

R

T

K
x
P

L
|
L

S

S

K
|
K

S

R

Q

H

G

G

D

A

S

T

G

S

L

V

G

E

E

H

L

F

R

R

A

R

Q

E

G

G

M

I

active site: S110 (= S16), K335 (= K267)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R12), A108 (= A14), P109 (= P15), G118 (= G24), T122 (≠ S28), E142 (= E48), Y276 (= Y208), R294 (= R226), G295 (= G227), D297 (= D229), H298 (≠ L230), L324 (= L256), I325 (= I257), L333 (= L265)
binding : T144 (≠ I50), D145 (= D51), R148 (= R54), Y208 (≠ S138), P213 (= P143), K252 (≠ T184), M255 (≠ I187), I266 (= I198), K269 (≠ R201), S270 (= S202), Y276 (= Y208), D297 (= D229), H298 (≠ L230), L299 (≠ F231), S300 (≠ H232), N301 (≠ A233), K304 (≠ V236), R330 (≠ G262), P332 (≠ K264)

Sites not aligning to the query:

binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
31% identity, 92% coverage: 12:281/293 of query aligns to 6:239/380 of 4g6zA

query
sites
4g6zA

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

N

T

G

G

P

F

L

I

H

H

L

L

G

G

H

N

A

I

L

R

S

S

A

A

L

L

M

Y

N

P

R

W

D

A

M

F

A

A

E

R

A

K

E

M

Q

K

A

G

R

T

L

F

L

V

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

Q

V

T

E

R

R

C

S

T

S

P

Q

E

E

F

A

E

V

A

D

G

A

I

I

L

L

R

E

D

G

L

M

E

A

W

W

L

L

D

G

I

L

G

D

W

Y

A

D

S

E

P

G

V

P

R

Y

Q

Q

S

M

E

Q

H

R

I

M

P

D

E

R

Y

Y

Q

R

A

E

A

V

L

L

H

A

A

Q

L

M

I

Q

E

E

R

K

G

G

L

L

V

V

Y

Y

P

P

A

C

F

Y

L

M

T

R

R

-

G

-

E

-

V

-

K

-

A

-

R

-

I

-

A

-

Y

Y

E

D

A

G

A

T

G

W

E

R

P

P

W

E

P

P

H

G

D

K

P

V

D

-

G

-

T

L

P

P

H

E

Y

P

P

P

A

A

S

G

D

-

R

-

E

V

R

A

P

P

A

V

D

L

E

R

R

F

R

R

D

N

M

P

L

L

T

T

S

G

G

-

K

-

H

-

A

-

W

-

R

-

L

-

D

-

M

-

R

-

K

-

A

-

L

-

D

-

L

-

I

-

G

-

E

-

L

T

L

V

F

A

W

W

T

D

E

D

T

A

G

V

D

K

G

G

R

R

T

V

G

-

E

E

I

I

A

S

A

N

E

E

P

E

D

-

V

-

W

L

G

D

D

D

V

L

I

V

L

V

S

A

R

R

S

P

D

D

A

G

P

T

S

P

S

M

Y

Y

H

N

L

F

S

C

V

V

D

D

A

L

L

D

Q

M

G

G

V

I

T

T

H

H

V

V

V

I

R
|
R

G

G

L

D

D

D

L

-

F

-

H
|
H

A

V

T

N

S

N

V

T

H

P

R

R

L

Q

L

I

Q

N

V

I

L

L

-

R

-

A

L

L

G

G

L

G

P

E

Q

V

P

P

V

V

Y

Y

H

A

H

H

H

L

R

P

L
x
T

I

V

L

L

G

N

A

E

D

Q

G

G

R

E

K

K

L

M

S

S

K
|
K

S

R

Q

H

G

G

D

A

S

M

G

S

L

V

G

M

E

G

L

Y

R

R

A

D

Q

A

G

G

active site: S10 (= S16), T214 (≠ L256), K225 (= K267)
binding glutamic acid: R6 (= R12), A8 (= A14), S10 (= S16), E42 (= E48), R184 (= R226), H188 (= H232)

8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
31% identity, 90% coverage: 12:274/293 of query aligns to 7:261/488 of 8vc5A

query
sites
8vc5A

R

R

F

I

A

A

P

P

S

S

P

P

N

T

G

G

P

D

L

P

H

H

L

V

G

G

H

T

A

A

L

Y

S

I

A

A

L

L

M

F

N

N

R

L

D

C

M

F

A

A

E

R

A

Q

E

H

Q

G

A

G

R

Q

L

F

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

Q

Q

T

L

R

R

C

S

T

T

P

R

E

E

F

S

E

E

A

Q

G

Q

I

I

L

Y

R

D

D

A

L

L

E

R

W

W

L

L

D

G

I

I

G

E

W

W

-

D

-

E

-

G

-

P

-

D

-

V

A

G

S

G

P

P

-

H

-

G

V

P

R

Y

R

R

Q

Q

S

S

E

E

H

R

I

G

P

H

E

I

Y

Y

Q

K

A

K

A

Y

L

S

H

D

A

E

L

L

I

V

E

E

R

K

G

G

L

H

V

A

Y

F

P

T

A
x
C

F

F

L
x
C

T

T

R

P

G

E

E

R

V

L

K

D

A

A

R

V

I

R

A

A

A

E

Y

Q

E

M

A

A

A

R

G

K

E

E

P

-

W

-

P

-

H

-

D

-

P

-

D

-

G

-

T

T

P

P

H

R

Y
|
Y

P

D

A

G

S

H

D
x
C

R

M

E

H

R

L

P

P

A

K

D

D

E

E

R

V

R

Q

D

R

M

R

L

L

T

A

S

A

G

G

K

E

K

S

H

H

A

V

W

T

R

R

L

M

D

K

M

V

-

P

R

T

K

E

A

G

L

V

D

C

L

V

I

V

G

P

E

D

L

M

L

L

F

-

W

-

T

-

E

-

T

-

G

-

D

-

G

-

R

-

T

R

G

G

E

D

I

V

A

E

A

I

E

P

P

W

D

D

V

R

W

M

G

D

D

M

V

Q

I

V

L

L

S

M

R

K

S

A

D

D

A

G

P

L

S

P

S

T

Y

Y

H

F

L

L

S

A

V

N

V

V

D

D

A

H

L

L

Q

M

G

G

V

I

T

T

H

H

V

V

V

L

R

R

G

G

L

E

D

E

L

W

F

L

H

P

A

S

T

A

S

P

V

K

H

L

R

I

L

K

L

L

Q

Y

V

E

L

Y

L

F

G

G

L

W

P

E

Q

Q

P

P

V

Q

Y

L

H

C

H

Y

H

M

R

P

L

L

I

L

L

R

G

N

A

P

D

D

G

K

R

S

K

K

L

L

S

S

K

K

S

R

Q

K

G

N

D

P

S

T

G

S

L

I

binding zinc ion: C107 (≠ A104), C109 (≠ L106), Y130 (= Y135), C134 (≠ D139)

3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
47% identity, 32% coverage: 10:102/293 of query aligns to 12:103/455 of 3aiiA

query
sites
3aiiA

V

V

F

L

R

R

F

F

A

A

P

P

S
x
N

P

P

N

S

G

G

P

P

L

L

H

H

L

I

G

G

H

H

A

A

L

R

S

A

A

A

L

I

M

L

N

N

R

H

D

E

M

Y

A

A

E

R

A

K

E

Y

Q

D

A

G

R

R

L

L

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

Q

P

T

R

R

R

C

V

T

D

P

P

E

E

F

A

E

Y

A

D

G

M

I

I

L

P

R

A

D

D

L

L

E

E

W

W

L

L

D

G

I

V

G

E

W

W

A

D

S

E

P

T

V

V

R

-

R

I

Q

Q

S

S

E

D

H

R

I

M

P

E

E

T

Y

Y

Q

Y

A

E

A

Y

L

T

H

E

A

K

L

L

I

I

E

E

R

R

G

G

L

G

V

A

Y

Y

active site: N18 (≠ S16)

Sites not aligning to the query:

active site: 231, 234
binding zinc ion: 105, 107, 122, 124

2re8A Glutaminyl-tRNA synthetase mutant c229r with bound analog 5'-o-[n-(l- glutamyl)-sulfamoyl]adenosine (see paper)
25% identity, 75% coverage: 12:230/293 of query aligns to 23:226/529 of 2re8A

query
sites
2re8A

R

R

F

F

A
x
P

P
|
P

S
x
E

P

P

N

N

G

G

P

Y

L

L

H

H

L

I

G
|
G

H
|
H

A

A

L

K

S

S

A

I

L

C

M

L

N

N

R

F

D

G

M

I

A

A

E

Q

A

D

E

Y

Q

K

A

G

R

Q

L

C

L

N

L

L

R

R

I

F

E
x
D

D

D

I
x
T

D
x
N

Q

P

T

V

R

K

C

E

T

D

P

I

E

E

F

Y

E

V

A

E

G

S

I

I

L

K

R

N

D

D

L

V

E

E

W

W

L

L

D

G

I

F

G

H

W

W

A

S

S

G

P

N

V

V

R

R

R

Y

Q

S

S

S

E

D

H

Y

I

F

P

D

E

Q

Y

L

Q

H

A

A

A

Y

L

A

H

I

A

E

L

L

I

I

E

N

R

K

G

G

L

L

V

A

Y

Y

P

V

A

D

F

E

L
|
L

T

T

R
x
P

G

E

E

Q

V

I

K
x
R

A

E

R

Y

I
x
R

A

G

A
x
T

Y
x
L

E

T

A

Q

A

P

G

G

E

K

P

N

W

S

P

P

H

Y

D

-

P

-

D

-

G

-

T

-

P

-

H

-

Y

-

P

-

A

-

S

-

D

-

R

R

E

D

R

R

P

S

A

V

D

E

E

E

R

N

-

L

-

A

-

L

R

F

D

E

M

K

L

M

T

R

S

A

G

G

K

G

K

F

H

E

A

E

W
x
G

R

K

L

A

D

C

M

L

R

R

K

A

A

K

L

I

D

D

L

M

I

A

G

S

E
x
P

L
x
F

L
x
I

F

V

W

M

T

R

E

D

T

P

G
x
V

D

L

G

Y

R
|
R

T

I

-

K

-

F

G

A

E

E

I

H

A

H

A

Q

E

T

P

G

D

N

V

K

W

W

G

C

D

I

V

Y

I

P

L
x
M

S

-

R

-

S

-

D

-

A

-

P

-

S

-

Y
|
Y

H

D

L

F

S

T

V
x
H

V

C

V

I

D

S

D

D

A

A

L

L

Q

E

G

G

V

I

T

T

H

H

V

S

V

L

R
|
R

G
x
T

L

L

D

E

L
x
F

binding o5'-(l-glutamyl-sulfamoyl)-adenosine: P25 (≠ A14), P26 (= P15), E27 (≠ S16), G35 (= G24), H36 (= H25), D59 (≠ E48), Y204 (= Y208), H208 (≠ V212), R222 (= R226), T223 (≠ G227)
binding : E27 (≠ S16), T61 (≠ I50), N62 (≠ D51), L117 (= L106), P119 (≠ R108), R123 (≠ K112), R126 (≠ I115), T128 (≠ A117), L129 (≠ Y118), G161 (≠ W159), P174 (≠ E172), F175 (≠ L173), I176 (≠ L174), V182 (≠ G180), R185 (= R183), M203 (≠ L199), Y204 (= Y208), F226 (≠ L230)

Sites not aligning to the query:

binding o5'-(l-glutamyl-sulfamoyl)-adenosine: 253, 254, 263
binding : 6, 228, 306, 309, 310, 311, 313, 314, 316, 319, 322, 329, 334, 362, 363, 392, 394, 395, 403, 405, 406, 435, 499, 501, 502, 527

Query Sequence

>WP_012759162.1 NCBI__GCF_000023185.1:WP_012759162.1
MTTSERQKPVFRFAPSPNGPLHLGHALSALMNRDMAEAEQARLLLRIEDIDQTRCTPEFE
AGILRDLEWLDIGWASPVRRQSEHIPEYQAALHALIERGLVYPAFLTRGEVKARIAAYEA
AGEPWPHDPDGTPHYPASDRERPADERRDMLTSGKKHAWRLDMRKALDLIGELLFWTETG
DGRTGEIAAEPDVWGDVILSRSDAPSSYHLSVVVDDALQGVTHVVRGLDLFHATSVHRLL
QVLLGLPQPVYHHHRLILGADGRKLSKSQGDSGLGELRAQGMSGADIRRLVGL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory