SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012759307.1 NCBI__GCF_000023185.1:WP_012759307.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2ay9A Aromatic amino acid aminotransferase with 5-phenylvaleric acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay9A

query
sites
2ay9A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

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S

A

L

L

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M

P

G

I

E

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F

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Q

A

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R

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K

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L

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G

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V

Y

Y

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K

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D

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G

T

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T

P

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I

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E

A

A

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E

E

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Q

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E

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E

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A

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G

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x
T

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G

A

A

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L

A

A

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E

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M

A

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D

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W
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W

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N

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H

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V

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S

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I

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M

E

N

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M

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Y

Y

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Y

Y

L

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D

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R

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G

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V

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D

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H

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P

P

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T

G

G

A

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D

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W

Q

A

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Q

G

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E

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E

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D

A

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A

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M

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P

E

E

M

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S
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S

C

C

S
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S

K
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K

N

N

F

F

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G

I

I

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Y

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R
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R

T

T

G

G

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C

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C

A

A

N

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A

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x
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G

A

A

A

K

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V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 5-phenylvaleric acid: Y65 (= Y65), W127 (= W127), N129 (= N129), N180 (= N180), S281 (= S281), F282 (≠ M282), R371 (= R371)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay8A Aromatic amino acid aminotransferase with 4-(2-thienyl)butyric acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay8A

query
sites
2ay8A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G
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G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

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R

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M

H

L

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E

A

T

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T

K

K

A

T

Y
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Y

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A

G

G

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L

A

S

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G

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E

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P

V

E

F

F

C

Q

D

K

L

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I

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G

E

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A

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A

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T

R

A

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T

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G
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G

A
x
T

G

G

A

A

L

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L

A

A

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G

E

L

L

I

A

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M

A

A

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P

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R

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W

V

P

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N

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H

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V

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S

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E

N

D

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M

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Y

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Y

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A

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T

G

R

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D

D

F

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A

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K

D

A

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K

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G

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D

I

M

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T

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S

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K
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K

N

N

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F

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G

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Y

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R
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R

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G

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C

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x
F

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V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 4-(2-thienyl)butyric acid: G34 (= G34), Y65 (= Y65), W127 (= W127), N180 (= N180), S281 (= S281), F282 (≠ M282), F345 (≠ I345), R371 (= R371)
binding pyridoxal-5'-phosphate: G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay7A Aromatic amino acid aminotransferase with 4-phenylbutyric acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay7A

query
sites
2ay7A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G

G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y
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Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G
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G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
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W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N

N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
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D

I

L

A
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A

Y
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Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

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R

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L

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I

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A

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P

E

E

M

V

L

L

V

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S

A

S
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S

C

C

S
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S

K
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K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
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R

T

T

G

G

A

C

A

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F

L

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A

C

A

A

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D

A

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S

M

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F

H

H

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G

A

A

A

K

I

I

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P

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E

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A

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W

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G

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A

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Y

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V

E

G

D

D

G

S

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R

I

I

N

N

V

I

A

A

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G

L

L

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A

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V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 4-phenyl-butanoic acid: Y65 (= Y65), S281 (= S281)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), T101 (≠ A101), W127 (= W127), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay6A Aromatic amino acid aminotransferase with 3-indolebutyric acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay6A

query
sites
2ay6A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G

G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y
|
Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S
|
S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R

R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 3-indolebutyric acid: Y65 (= Y65), S281 (= S281)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay5A Aromatic amino acid aminotransferase with 3-indolepropionic acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay5A

query
sites
2ay5A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G

G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y
|
Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S
|
S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R

R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding indolylpropionic acid: Y65 (= Y65), S281 (= S281)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay3A Aromatic amino acid aminotransferase with 3-(3,4-dimethoxyphenyl) propionic acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay3A

query
sites
2ay3A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G
|
G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y

Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N
|
N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S

S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R
|
R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 3-(3,4-dimethoxyphenyl)propionic acid: G34 (= G34), W127 (= W127), N129 (= N129), N180 (= N180), R371 (= R371)
binding pyridoxal-5'-phosphate: G99 (= G99), G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay1A Aromatic amino acid aminotransferase with 4-aminohydrocinnamic acid (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 2ay1A

query
sites
2ay1A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G

G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y
|
Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G

G

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S
|
S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R

R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding 4-aminohydrocinnamic acid: Y65 (= Y65), S281 (= S281)
binding pyridoxal-5'-phosphate: G100 (= G100), T101 (≠ A101), W127 (= W127), N180 (= N180), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

1ay4A Aromatic amino acid aminotransferase without substrate (see paper)
47% identity, 99% coverage: 1:392/396 of query aligns to 1:392/394 of 1ay4A

query
sites
1ay4A

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

R

Q

A

D

D

S

P

R

R

S

Q

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G

G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y

Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G

G

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N

N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S

S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R

R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W127 (= W127), D208 (= D208), A210 (= A210), K243 (= K243)
binding pyridoxal-5'-phosphate: G100 (= G100), T101 (≠ A101), W127 (= W127), D208 (= D208), A210 (= A210), Y211 (= Y211), S240 (= S240), S242 (= S242), K243 (= K243), R251 (= R251)

2ay4B Aromatic amino acid aminotransferase with 3-(p-tolyl)propionic acid (see paper)
46% identity, 99% coverage: 1:392/396 of query aligns to 1:386/388 of 2ay4B

query
sites
2ay4B

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I
|
I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

-

Q

-

D

-

S

-

R

-

S

-

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G
|
G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y

Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G

G

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N
|
N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S

S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R
|
R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W121 (= W127), D202 (= D208), A204 (= A210), K237 (= K243)
binding pyridoxal-5'-phosphate: G94 (= G100), T95 (≠ A101), W121 (= W127), N174 (= N180), D202 (= D208), A204 (= A210), Y205 (= Y211), S234 (= S240), S236 (= S242), K237 (= K243), R245 (= R251)
binding 3-(p-tolyl)propionic acid: I13 (= I13), G28 (= G34), W121 (= W127), N123 (= N129), R365 (= R371)

2ay2B Aromatic amino acid aminotransferase with cyclohexane propionic acid (see paper)
46% identity, 99% coverage: 1:392/396 of query aligns to 1:386/388 of 2ay2B

query
sites
2ay2B

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

-

Q

-

D

-

S

-

R

-

S

-

N

G

K

K

I

I

D

D

L

L

G

G

V

V

G
|
G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y

Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G

G

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N

N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S

S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R
|
R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W121 (= W127), D202 (= D208), A204 (= A210), K237 (= K243)
binding cyclohexane propionic acid: G28 (= G34), W121 (= W127), R365 (= R371)
binding pyridoxal-5'-phosphate: G94 (= G100), T95 (≠ A101), W121 (= W127), N174 (= N180), D202 (= D208), A204 (= A210), Y205 (= Y211), S234 (= S240), S236 (= S242), K237 (= K243), R245 (= R251)

1ay8B Aromatic amino acid aminotransferase complex with 3-phenylpropionate (see paper)
46% identity, 99% coverage: 1:392/396 of query aligns to 1:386/388 of 1ay8B

query
sites
1ay8B

M

M

F

L

D

G

D

N

L

L

I

K

M

P

P

Q

P

A

A

P

D

D

K

K

I

I

L

L

S

A

L

L

M

M

P

G

I

E

F

F

R

-

Q

-

D

-

S

-

R

-

S

-

N

G

K

K

I

I

D

D

L

L

G

G

V
|
V

G
|
G

V

V

Y

Y

R

K

D

D

A

A

S

T

G

G

T

H

T

T

P

P

I

I

P

M

R

R

A

A

V

V

R

H

E

A

A

A

E

E

K

Q

R

R

I

M

H

L

T

E

A

T

Q

E

T

T

K

K

A

T

Y

Y

V

A

G

G

P

L

A

S

G

G

D

E

P

P

V

E

F

F

C

Q

D

K

L

A

I

M

G

G

R

E

L

L

V

I

F

L

G

G

E

D

A

G

A

L

P

K

W

S

E

E

R

T

I

T

R

A

G

T

I

L

Q

A

T

T

P

V

G

G

G
|
G

A
x
T

G

G

A

A

L

L

T

R

V

Q

L

A

A

L

G

E

L

L

I

A

S

R

L

M

A

A

R

N

P

P

G

D

A

L

A

R

V

V

H

F

V

V

P

S

D

D

P

P

T

T

W
|
W

V

P

N
|
N

H

H

V

V

S

S

I

I

L

M

E

N

D

F

N

M

R

G

L

L

R

P

V

V

V

Q

T

T

Y

Y

P

R

Y

Y

L

F

D

D

R

A

R

E

T

T

G

R

E

G

V

V

D

D

F

F

D

E

A

G

L

M

L

K

D

A

H

D

F

L

S

A

R

A

S

A

E

K

R

K

G

G

D

D

I

M

V

V

L

L

H

H

G

G

C

C

H

H

N
|
N

P

P

T

T

G

G

A

A

D

N

P

L

S

T

R

L

S

D

Q

Q

W

W

Q

A

A

E

L

I

A

A

E

S

I

I

I

L

A

E

E

K

R

T

G

G

L

A

V

L

P

P

L

L

V

I

D
|
D

I

L

A
|
A

Y
|
Y

Q

Q

G

G

F

F

G

G

E

D

G

G

L

L

E

E

D

E

D

D

A

A

F

A

V

G

V

T

R

R

L

L

L

I

T

A

G

S

M

R

V

I

P

P

E

E

M

V

L

L

V

I

S

A

S
|
S

C

C

S
|
S

K
|
K

N

N

F

F

G

G

I

I

Y

Y

R

R

E

E

R
|
R

T

T

G

G

A

C

A

L

F

L

I

A

L

L

A

C

A

A

N

D

A

A

D

A

R

T

A

R

D

E

A

L

A

A

K

Q

A

G

Q

A

L

M

T

A

V

F

R

L

A

N

R

R

L

Q

V

T

Y

Y

S

S

M

F

P

P

D

F

H

H

G

G

A

A

A

K

I

I

V

V

R

S

T

T

V

V

L

L

E

T

D

T

P

P

A

E

L

L

S

R

A

A

D

D

W

W

R

M

A

A

E

E

L

L

D

E

D

A

M

V

R

R

S

S

S

G

I

M

L

L

S

R

L

L

R

R

Q

E

G

Q

L

L

A

A

A

G

S

E

F

L

R

R

R

D

F

L

T

S

N

G

G

S

S

D

D

R

Y

F

D

G

F

F

L

V

A

A

K

E

N

H

K

R

G

G

M

M

I

F

S

S

L

R

I

L

G

G

L

A

T

T

P

P

G

E

E

Q

A

V

V

K

M

R

L

I

R

K

E

E

Q

E

H

F

A

G

I

I

Y

Y

I

M

V

V

E

G

D

D

G

S

R
|
R

I

I

N

N

V

I

A

A

G

G

L

L

Q

N

A

D

S

N

Q

T

I

I

D

P

T

I

F

L

A

A

E

R

A

A

V

I

L

I

A

E

V

V

active site: W121 (= W127), D202 (= D208), A204 (= A210), K237 (= K243)
binding hydrocinnamic acid: V27 (= V33), G28 (= G34), W121 (= W127), N123 (= N129), R365 (= R371)
binding pyridoxal-5'-phosphate: G94 (= G100), T95 (≠ A101), W121 (= W127), N174 (= N180), D202 (= D208), A204 (= A210), Y205 (= Y211), S234 (= S240), S236 (= S242), K237 (= K243), R245 (= R251)

5vwrA E.Coli aspartate aminotransferase-(1r,3s,4s)-3-amino-4- fluorocyclopentane-1-carboxylic acid (fcp)-alpha-ketoglutarate (see paper)
43% identity, 99% coverage: 1:392/396 of query aligns to 1:395/396 of 5vwrA

query
sites
5vwrA

M

M

F

F

D

E

D

N

L

I

I

T

M

A

P

A

P

P

A

A

D

D

K

P

I
|
I

L

L

S

G

L

L

M

A

P

D

I

L

F

F

R

R

Q

A

D

D

S

E

R

R

S

P

N

G

K

K

I

I

D

N

L

L

G

G

V

I

G
|
G

V

V

Y

Y

R

K

D

D

A

E

S

T

G

G

T

K

T

T

P

P

I

V

P

L

R

T

A

S

V

V