SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012759370.1 NCBI__GCF_000023185.1:WP_012759370.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
28% identity, 90% coverage: 8:102/106 of query aligns to 3:101/104 of 2gtvX

query
sites
2gtvX

S

E

Q

K

L

L

A

A

S

E

Y

I

R
|
R

Q

K

S

K

I

I

D

D

N

E

I

I

D

D

A

N

A

K

L

I

V

L

H

K

-

A

-

R

-

W

-

P

-

W

-

A

-

E

-

K

M

L

L

I

A

A

E

E

R
|
R

F

N

R

S

C

L

T

A

K

K

E

D

V
|
V

G

A

V

E

L

I

K
|
K

A

N

K

Q

Y

L

N

G

L

I

P

P

P

I

A

N

D
|
D

P

P

A

E

R
|
R

E
|
E

E

K

Y

Y

Q

I

I

Y

E

D

R

R

L

I

R

R

Q

K

L

L

A

C

K

K

A

E

A

H

N

N

L

V

D

D

P

E

D

N

F

I

A

G

E

I

K

K

F

-

L

-

N

-

F

-

V

I

I

F

K

Q

E

R

V

L

I

I

R

E

H

H

H
x
N

E

K

Q

A

I

L

A
x
Q

A

K

D

Q

H

Y

A

L

E

E

Q

E

S

T

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R9 (= R14), R34 (= R31), V41 (= V38), K45 (= K42), D54 (= D51), R57 (= R54), E58 (= E55), N90 (≠ H91), Q94 (≠ A95)

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
30% identity, 84% coverage: 10:98/106 of query aligns to 2:90/95 of 1ecmB

query
sites
1ecmB

L

L

A

L

S

A

Y

L

R

R

Q

E

S

K

I

I

D

S

N

A

I

L

D

D

A

E

A

K

L

L

V

L

H

A

M

L

L

L

A

A

E

E

R
|
R

F

R

R

E

C

L

T

A

K

V

E

E

V
|
V

G

G

V

K

L

A

K
|
K

A

L

K

L

Y

S

N

H

L

R

P

P

P

V

A
x
R

D
|
D

P

I

A

D

R
|
R

E
|
E

E

R

Y

D

Q

L

I

L

E

E

R

R

L

L

R

I

Q

T

L

L

A

G

K

K

A

A

A

H

N

H

L

L

D

D

P

A

D

H

F

Y

A

I

E

T

K

R

F

L

L

F

N

Q

F

L

V

I

I
|
I

K

E

E

D

V
x
S

I

V

R

L

H

T

H
x
Q

E

Q

Q

A

I

L

A

L

A

Q

D

Q

H

H

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R31), V30 (= V38), K34 (= K42), R42 (≠ A50), D43 (= D51), R46 (= R54), E47 (= E55), I76 (= I84), S79 (≠ V87), Q83 (≠ H91)

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
30% identity, 84% coverage: 10:98/106 of query aligns to 7:95/386 of P0A9J8

query
sites
P0A9J8

L

L

A

L

S

A

Y

L

R
|
R

Q

E

S

K

I

I

D

S

N

A

I

L

D

D

A

E

A

K

L

L

V

L

H

A

M

L

L

L

A

A

E

E

R
|
R

F

R

R

E

C

L

T

A

K

V

E

E

V

V

G

G

V

K

L

A

K
|
K

A

L

K

L

Y

S

N

H

L

R

P

P

P

V

A

R

D

D

P

I

A

D

R

R

E
|
E

E

R

Y

D

Q

L

I

L

E

E

R

R

L

L

R

I

Q

T

L

L

A

G

K

K

A

A

A

H

N

H

L

L

D

D

P

A

D

H

F

Y

A

I

E

T

K

R

F

L

L

F

N

Q

F

L

V

I

I

I

K

E

E

D

V

S

I

V

R

L

H

T

H
x
Q

E

Q

Q

A

I

L

A

L

A

Q

D

Q

H

H

R11 (= R14) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R31) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K42) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E55) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.
Q88 (≠ H91) mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

Query Sequence

>WP_012759370.1 NCBI__GCF_000023185.1:WP_012759370.1
MIDPNVKSQLASYRQSIDNIDAALVHMLAERFRCTKEVGVLKAKYNLPPADPAREEYQIE
RLRQLAKAANLDPDFAEKFLNFVIKEVIRHHEQIAADHAEQSAAAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory