SitesBLAST
Comparing WP_012759746.1 NCBI__GCF_000023185.1:WP_012759746.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
6torB Human o-phosphoethanolamine phospho-lyase (see paper)
41% identity, 41% coverage: 571:969/973 of query aligns to 2:394/404 of 6torB
5g4jA Phospholyase a1rdf1 from arthrobacter in complex with phosphoethanolamine (see paper)
34% identity, 43% coverage: 550:966/973 of query aligns to 2:415/423 of 5g4jA
- active site: S14 (≠ Q562), Y130 (= Y678), D201 (≠ E752), D234 (= D785), Q237 (= Q788), K264 (= K815), T294 (≠ S845), K395 (= K946)
- binding {5-hydroxy-6-methyl-4-[(E)-{[2-(phosphonooxy)ethyl]imino}methyl]pyridin-3-yl}methyl dihydrogen phosphate: Y44 (≠ V592), R73 (= R621), G103 (= G651), S104 (= S652), Y130 (= Y678), H131 (= H679), D234 (= D785), V236 (= V787), Q237 (= Q788), K264 (= K815), T294 (≠ S845), R397 (≠ K948)
5g4iA Plp-dependent phospholyase a1rdf1 from arthrobacter aurescens tc1 (see paper)
34% identity, 43% coverage: 550:966/973 of query aligns to 2:415/423 of 5g4iA
- active site: S14 (≠ Q562), Y130 (= Y678), D201 (≠ E752), D234 (= D785), Q237 (= Q788), K264 (= K815), T294 (≠ S845), K395 (= K946)
- binding pyridoxal-5'-phosphate: G103 (= G651), S104 (= S652), H131 (= H679), D234 (= D785), V236 (= V787), Q237 (= Q788), K264 (= K815)
- binding phosphate ion: Y44 (≠ V592), R397 (≠ K948)
1d7vA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with nma (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1d7vA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding potassium ion: H75 (vs. gap), L76 (≠ T618), F77 (≠ N619), S78 (= S620), T301 (= T846), H302 (≠ G847), V303 (≠ G848), S304 (= S849), D305 (vs. gap)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-2-methylalanine: A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), S213 (≠ N757), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1d7uA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with lcs (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1d7uA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding potassium ion: H75 (vs. gap), L76 (≠ T618), F77 (≠ N619), S78 (= S620), T301 (= T846), H302 (≠ G847), V303 (≠ G848), S304 (= S849), D305 (vs. gap)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1d7sA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with dcs (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1d7sA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Q50 (vs. gap), G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
- binding potassium ion: H75 (vs. gap), L76 (≠ T618), F77 (≠ N619), S78 (= S620), T301 (= T846), H302 (≠ G847), V303 (≠ G848), S304 (= S849), D305 (vs. gap)
1d7rA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with 5pa (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1d7rA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), S213 (≠ N757), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
- binding potassium ion: H75 (vs. gap), L76 (≠ T618), F77 (≠ N619), S78 (= S620), T301 (= T846), H302 (≠ G847), V303 (≠ G848), S304 (= S849), D305 (vs. gap)
1zobA Crystal structure of dialkylglycine decarboxylases bound with calcium ion
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1zobA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding calcium ion: L76 (≠ T618), S78 (= S620), V303 (≠ G848), S304 (= S849), D305 (vs. gap)
- binding pyridoxal-5'-phosphate: T108 (≠ S650), A110 (≠ S652), N113 (= N655), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815)
1zc9A The crystal structure of dialkylglycine decarboxylase complex with pyridoxamine 5-phosphate (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1zc9A
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815)
1m0qA Structure of dialkylglycine decarboxylase complexed with s-1- aminoethanephosphonate (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1m0qA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding (1s)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]ethylphosphonic acid: Q50 (vs. gap), T108 (≠ S650), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), S213 (≠ N757), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1m0pA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- phenylethanephosphonate (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1m0pA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-phenylethylphosphonic acid: Q50 (vs. gap), T108 (≠ S650), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), S213 (≠ N757), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1m0oA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- methylpropanephosphonate (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1m0oA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-methylpropylphosphonic acid: Q50 (vs. gap), G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1m0nA Structure of dialkylglycine decarboxylase complexed with 1- aminocyclopentanephosphonate (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1m0nA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding 1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]cyclopentylphosphonic acid: Q50 (vs. gap), M51 (vs. gap), A110 (≠ S652), N113 (= N655), W136 (≠ Y678), H137 (= H679), E208 (= E752), S213 (≠ N757), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815), R404 (≠ K946)
1dgdA An alkali metal ion size-dependent switch in the active site structure of dialkylglycine decarboxylase (see paper)
33% identity, 44% coverage: 546:971/973 of query aligns to 3:429/431 of 1dgdA
- active site: G19 (≠ Q562), W136 (≠ Y678), E208 (= E752), D241 (= D785), Q244 (= Q788), K270 (= K815), T301 (= T846), R404 (≠ K946)
- binding pyridoxal-5'-phosphate: T108 (≠ S650), G109 (= G651), A110 (≠ S652), W136 (≠ Y678), H137 (= H679), E208 (= E752), D241 (= D785), A243 (≠ V787), Q244 (= Q788), K270 (= K815)
5wyaA Structure of amino acid racemase, 2.65 a (see paper)
30% identity, 43% coverage: 546:959/973 of query aligns to 4:419/439 of 5wyaA
- active site: A20 (≠ Q562), Y140 (= Y678), E215 (= E752), D248 (= D785), N251 (≠ Q788), K278 (= K815), T307 (≠ S845), R406 (≠ K946)
- binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ V595), Y82 (≠ R621), S112 (= S650), G113 (= G651), S114 (= S652), Y140 (= Y678), H141 (= H679), E215 (= E752), D248 (= D785), V250 (= V787), N251 (≠ Q788), K278 (= K815), F306 (= F843), T307 (≠ S845), R406 (≠ K946)
5wyfA Structure of amino acid racemase, 2.12 a (see paper)
30% identity, 43% coverage: 546:959/973 of query aligns to 6:421/446 of 5wyfA
- active site: A22 (≠ Q562), Y142 (= Y678), E217 (= E752), D250 (= D785), N253 (≠ Q788), K280 (= K815), T309 (≠ S845), R408 (≠ K946)
- binding n-[o-phosphono-pyridoxyl]-isoleucine: A54 (≠ V595), Y84 (≠ R621), G115 (= G651), S116 (= S652), Y142 (= Y678), H143 (= H679), D222 (≠ N757), D250 (= D785), V252 (= V787), N253 (≠ Q788), K280 (= K815), F308 (= F843), T309 (≠ S845), R408 (≠ K946)
4ysnC Structure of aminoacid racemase in complex with plp (see paper)
30% identity, 43% coverage: 546:959/973 of query aligns to 13:428/448 of 4ysnC
- active site: A29 (≠ Q562), Y149 (= Y678), E224 (= E752), D257 (= D785), N260 (≠ Q788), K287 (= K815), T316 (≠ S845), R415 (≠ K946)
- binding pyridoxal-5'-phosphate: S121 (= S650), G122 (= G651), S123 (= S652), Y149 (= Y678), H150 (= H679), E224 (= E752), D257 (= D785), V259 (= V787), K287 (= K815), F315 (= F843), T316 (≠ S845)
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
31% identity, 42% coverage: 555:965/973 of query aligns to 8:431/443 of 6fyqA
- active site: F15 (vs. gap), Y147 (= Y678), D243 (= D785), K272 (= K815)
- binding pyridoxal-5'-phosphate: G114 (= G651), S115 (= S652), Y147 (= Y678), H148 (= H679), G149 (= G680), E210 (= E752), D243 (= D785), V245 (= V787), I246 (≠ Q788), K272 (= K815)
O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
30% identity, 46% coverage: 527:971/973 of query aligns to 16:460/474 of O58478
- D251 (≠ N757) mutation to A: Loss of activity.
- K308 (= K815) mutation to A: Loss of activity.
7vo1A Structure of aminotransferase-substrate complex (see paper)
29% identity, 45% coverage: 536:970/973 of query aligns to 1:441/452 of 7vo1A
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (≠ V595), S121 (= S650), G122 (= G651), T123 (≠ S652), F149 (≠ Y678), H150 (= H679), R152 (≠ W681), E234 (≠ N757), D262 (= D785), V264 (= V787), Q265 (= Q788), K291 (= K815), N318 (≠ S844), T319 (≠ S845), R417 (≠ K946)
Query Sequence
>WP_012759746.1 NCBI__GCF_000023185.1:WP_012759746.1
MTDEALVDRMALPRPDVTATDAEEILLAHYSLSGTLAELGSQQDRNYRVDSERGRYVLKI
CHAAYDIRELEAQNAAIRHLKSRQDAPRVPKVIPTNEGREIVVLTVRGQGYQVRLLEYLE
GEGLTELTYLAPASVAALGALCARLAQALADFNHPGLDRSLQWDLRRAGPVAVQLLSAIT
DSAARDRIAKTMVMAVRRIQPLAPALRLQAVHHDVTGDNVVGHRDAHGHIIPDGVIDFGD
IIRGWLVGDLAVTCASLLHQADGDPFHILPAVTAYQAIYPLSEEELKALWPLIVARAVIL
VASGEQQISVDPDNDYVRDNLDRERAIFDTAMSVPFDLMEAAILKAAGVDVTAPETSGWL
PLLPDIDPAGIAYVDLGVRSPHFSAGNWLNTDMDWRLLARMATENGTAATRYGEYRLSRA
GTAKGQATCALHVDICLAAGSAIAAPFAGRIGWKDQHLTLASDTMTLHLDGLDLSVEDGA
EIAAGDSLGTVFGEASSLGGLRVQLCSVAGLEPPLFATPRTAAAWSVLCPSPSLLLSPQA
DAPQPETAKLFARRRAHLARPQKNYYAAPPQIERGWKEHLFDVEGRAYLDMVNNVTILGH
GHPKLAAAISAQWLRLNTNSRFHYAAITEFSERLAALSPDGLDAVFLVNSGSEANDLALR
LAQAHSGARNMLCLLEAYHGWSAASDAVSTSIADNPQAPTTRPDWVHTIVSPNTYRGDFR
GPDTAADYLGMATPVLEAIDAAGEGLAGFIAESVYGNAGGIPLPEGYLKELYAQVRARGG
VCIADEVQVGYARLGHYFWGFEQQGVVPDIITVAKGMGNGHPLGAVITTREIAQSLEKEG
TFFSSTGGSPVSCIAGMTVLDIMAEEKLQENARAVGDHLKARLAALIDRHPIAGAVHGMG
LYLGLEFVRDRTTLEPATEETAAICDRLLDLGVIMQPTGDHQNVLKIKPPLCLSIDSADF
FADTLEKVLEEGW
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory