SitesBLAST
Comparing WP_012759947.1 NCBI__GCF_000023185.1:WP_012759947.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3hrdD Crystal structure of nicotinate dehydrogenase (see paper)
43% identity, 86% coverage: 5:153/174 of query aligns to 4:152/160 of 3hrdD
- binding flavin-adenine dinucleotide: E44 (≠ L45), G45 (= G46), E46 (= E47)
- binding fe2/s2 (inorganic) cluster: E40 (≠ Y41), C42 (= C43), S43 (≠ G44), G45 (= G46), C47 (= C48), G48 (= G49), C50 (= C51), C62 (= C63), Q100 (= Q101), C101 (= C102), G102 (= G103), C104 (= C105), C136 (= C137), C138 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C138 (= C139)
Q0QLF3 Nicotinate dehydrogenase small FeS subunit; NDH; Nicotinic acid hydroxylase small FeS subunit; NAH; EC 1.17.1.5 from Eubacterium barkeri (Clostridium barkeri) (see paper)
43% identity, 86% coverage: 5:153/174 of query aligns to 4:152/157 of Q0QLF3
- C42 (= C43) binding [2Fe-2S] cluster
- C47 (= C48) binding [2Fe-2S] cluster
- C50 (= C51) binding [2Fe-2S] cluster
- C62 (= C63) binding [2Fe-2S] cluster
- C101 (= C102) binding [2Fe-2S] cluster
- C104 (= C105) binding [2Fe-2S] cluster
- C136 (= C137) binding [2Fe-2S] cluster
- C138 (= C139) binding [2Fe-2S] cluster
4zohC Crystal structure of glyceraldehyde oxidoreductase (see paper)
39% identity, 85% coverage: 5:152/174 of query aligns to 10:156/161 of 4zohC
- binding fe2/s2 (inorganic) cluster: C47 (= C43), S50 (≠ G46), C52 (= C48), G53 (= G49), C55 (= C51), C67 (= C63), C106 (= C102), G107 (= G103), C109 (= C105), C141 (= C137), C143 (= C139)
- binding pterin cytosine dinucleotide: Q105 (= Q101), C143 (= C139)
8gy3B Cryo-em structure of membrane-bound aldehyde dehydrogenase from gluconobacter oxydans
39% identity, 89% coverage: 5:159/174 of query aligns to 1:154/154 of 8gy3B
- binding fe2/s2 (inorganic) cluster: G38 (= G42), C39 (= C43), G40 (= G44), G42 (= G46), C44 (= C48), G45 (= G49), C47 (= C51), C59 (= C63), C97 (= C102), C100 (= C105), Q101 (≠ L106), C132 (= C137), C134 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q96 (= Q101), C134 (= C139)
8uemC The cryoem structure of the high affinity carbon monoxide dehydrogenase from mycobacterium smegmatis
39% identity, 85% coverage: 5:152/174 of query aligns to 1:148/153 of 8uemC
- binding fe2/s2 (inorganic) cluster: W37 (≠ Y41), G38 (= G42), C39 (= C43), S42 (≠ G46), N43 (≠ E47), C44 (= C48), G45 (= G49), C47 (= C51), K57 (≠ R61), C59 (= C63), C98 (= C102), G99 (= G103), C101 (= C105), C133 (= C137), C135 (= C139)
- binding pterin cytosine dinucleotide: Q97 (= Q101), C135 (= C139)
1ffuD Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava which lacks the mo-pyranopterin moiety of the molybdenum cofactor (see paper)
40% identity, 84% coverage: 7:152/174 of query aligns to 5:150/156 of 1ffuD
- binding fe2/s2 (inorganic) cluster: C41 (= C43), S44 (≠ G46), H45 (≠ E47), C46 (= C48), G47 (= G49), C49 (= C51), C61 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
1ffvA Carbon monoxide dehydrogenase from hydrogenophaga pseudoflava (see paper)
40% identity, 84% coverage: 7:152/174 of query aligns to 4:149/155 of 1ffvA
- binding fe2/s2 (inorganic) cluster: I38 (≠ Y41), C40 (= C43), S43 (≠ G46), C45 (= C48), G46 (= G49), C48 (= C51), C60 (= C63), C99 (= C102), G100 (= G103), C102 (= C105), C134 (= C137), C136 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q98 (= Q101), C136 (= C139)
1dgjA Crystal structure of the aldehyde oxidoreductase from desulfovibrio desulfuricans atcc 27774 (see paper)
36% identity, 96% coverage: 8:174/174 of query aligns to 5:174/906 of 1dgjA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), G39 (= G42), C40 (= C43), G41 (= G44), G43 (= G46), Q44 (≠ E47), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding pterin cytosine dinucleotide: Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 391, 427, 503, 507, 535, 869, 870
- binding molybdenum (iv)oxide: 424, 535, 698, 869
- binding pterin cytosine dinucleotide: 423, 424, 535, 652, 655, 656, 657, 658, 697, 698, 700, 702, 703, 799, 800, 803, 804, 807, 865, 866, 867, 868, 869
5y6qA Crystal structure of an aldehyde oxidase from methylobacillus sp. Ky4400 (see paper)
38% identity, 85% coverage: 5:152/174 of query aligns to 3:151/157 of 5y6qA
- binding fe2/s2 (inorganic) cluster: G40 (= G42), C41 (= C43), D42 (≠ G44), G44 (= G46), C46 (= C48), G47 (= G49), C49 (= C51), C61 (= C63), C101 (= C102), G102 (= G103), C104 (= C105), C136 (= C137), C138 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C138 (= C139)
1sb3C Structure of 4-hydroxybenzoyl-coa reductase from thauera aromatica (see paper)
44% identity, 73% coverage: 26:152/174 of query aligns to 24:150/161 of 1sb3C
- binding fe2/s2 (inorganic) cluster: Q39 (≠ Y41), C41 (= C43), G44 (= G46), C46 (= C48), G47 (= G49), C49 (= C51), C61 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C135 (= C137), C137 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C137 (= C139)
4usaA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with trans-cinnamaldehyde (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 4usaA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 531, 532, 535, 539
- binding hydrocinnamic acid: 255, 425, 494, 497, 535, 626
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us9A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with 3- phenylpropionaldehyde (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 4us9A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 3-phenylpropanal: 255, 257, 258, 752
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4us8A Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with benzaldehyde (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 4us8A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 532, 535, 539
- binding benzaldehyde: 255, 255, 394, 425, 425, 425, 425, 497, 497, 501, 531, 535, 535, 626, 626, 626, 694, 696, 697
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 697, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
4c7yA Aldehyde oxidoreductase from desulfovibrio gigas (mop), soaked with sodium dithionite and sodium sulfide (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 4c7yA
- binding fe2/s2 (inorganic) cluster: C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding bicarbonate ion: 460, 498, 531, 535, 539
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
- binding hydrogen peroxide: 696, 697, 869
3fc4A Ethylene glycol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 3fc4A
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding 1,2-ethanediol: 535, 622, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
3fahA Glycerol inhibited form of aldehyde oxidoreductase from desulfovibrio gigas (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 3fahA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding glycerol: 416, 535, 622, 683, 696, 697, 869, 884, 889, 890, 892
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 419, 420, 421, 422, 533, 650, 653, 654, 655, 656, 695, 696, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
1sijA Crystal structure of the aldehyde dehydrogenase (a.K.A. Aor or mop) of desulfovibrio gigas covalently bound to [aso3]- (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of 1sijA
- binding fe2/s2 (inorganic) cluster: V38 (≠ Y41), C40 (= C43), E41 (≠ G44), G43 (= G46), C45 (= C48), G46 (= G49), C48 (= C51), R58 (= R61), C60 (= C63), Q99 (= Q101), C100 (= C102), G101 (= G103), C103 (= C105), C137 (= C137), C139 (= C139)
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): Q99 (= Q101), C139 (= C139)
Sites not aligning to the query:
- active site: 390, 425, 501, 505, 533, 869, 870
- binding arsenite: 535, 696, 697, 869
- binding magnesium ion: 899, 903
- binding (molybdopterin-cytosine dinucleotide-s,s)-dioxo-aqua-molybdenum(v): 420, 421, 422, 533, 653, 654, 655, 656, 695, 696, 698, 700, 701, 799, 800, 804, 807, 865, 866, 867, 868, 869
Q46509 Aldehyde oxidoreductase; Molybdenum iron sulfur protein; EC 1.2.99.7 from Megalodesulfovibrio gigas (Desulfovibrio gigas) (see paper)
35% identity, 88% coverage: 9:161/174 of query aligns to 6:161/907 of Q46509
- C40 (= C43) binding [2Fe-2S] cluster
- C45 (= C48) binding [2Fe-2S] cluster
- C48 (= C51) binding [2Fe-2S] cluster
- C60 (= C63) binding [2Fe-2S] cluster
- C100 (= C102) binding [2Fe-2S] cluster
- C103 (= C105) binding [2Fe-2S] cluster
- C137 (= C137) binding [2Fe-2S] cluster
- C139 (= C139) binding [2Fe-2S] cluster
7dqxC Crystal structure of xanthine dehydrogenase family protein
37% identity, 82% coverage: 7:149/174 of query aligns to 6:149/160 of 7dqxC
- binding fe2/s2 (inorganic) cluster: C42 (= C43), G45 (= G46), V46 (≠ E47), C47 (= C48), C50 (= C51), R60 (= R61), C62 (= C63), Q100 (= Q101), C101 (= C102), C104 (= C105), C137 (= C137), C139 (= C139)
- binding pterin cytosine dinucleotide: Q100 (= Q101), C139 (= C139)
P77165 Aldehyde oxidoreductase iron-sulfur-binding subunit PaoA; EC 1.2.99.6 from Escherichia coli (strain K12) (see paper)
35% identity, 87% coverage: 7:158/174 of query aligns to 63:229/229 of P77165
- C99 (= C43) binding [2Fe-2S] cluster
- C104 (= C48) binding [2Fe-2S] cluster
- G105 (= G49) binding [2Fe-2S] cluster
- C107 (= C51) binding [2Fe-2S] cluster
- C119 (= C63) binding [2Fe-2S] cluster
- C158 (= C102) binding [2Fe-2S] cluster
- C161 (= C105) binding [2Fe-2S] cluster
- C208 (= C137) binding [2Fe-2S] cluster
- C210 (= C139) binding [2Fe-2S] cluster
Query Sequence
>WP_012759947.1 NCBI__GCF_000023185.1:WP_012759947.1
MKPPVSVSLNVNDALWRVSAKPETPLLYILRNDLCLNGPKYGCGLGECGACAVLVDGRPV
RSCTVPLGAIGKRRVITLEGLAVDGLLHPVQQAFIEEAAAQCGYCLNGMIIATVGLLQRN
TDPDESDIRDALRHHLCRCGTHVEILAAVRRAVTLTKAGRPVDAMTDDMTEASR
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory