SitesBLAST
Comparing WP_012760746.1 NCBI__GCF_000023185.1:WP_012760746.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ktoA Crystal structure of a putative isovaleryl-coa dehydrogenase (psi- nysgrc-012251) from sinorhizobium meliloti 1021
68% identity, 99% coverage: 2:380/381 of query aligns to 6:376/377 of 4ktoA
- active site: M130 (= M126), S131 (= S127), E239 (= E243), A360 (= A364), R372 (= R376)
- binding flavin-adenine dinucleotide: L128 (= L124), M130 (= M126), S131 (= S127), M155 (≠ F156), W156 (= W157), T158 (= T159), R265 (= R269), F268 (= F272), I272 (= I276), F275 (= F279), M278 (= M282), Q333 (= Q337), A334 (= A338), G337 (= G341), L355 (= L359), G359 (= G363), T362 (= T366), E364 (= E368)
1ivhA Structure of human isovaleryl-coa dehydrogenase at 2.6 angstroms resolution: structural basis for substrate specificity (see paper)
61% identity, 98% coverage: 6:380/381 of query aligns to 8:386/387 of 1ivhA
- active site: M130 (= M126), S131 (= S127), E249 (= E243), A370 (= A364), R382 (= R376)
- binding coenzyme a persulfide: S137 (= S133), S185 (vs. gap), R186 (≠ K180), V239 (= V233), Y240 (≠ K234), M243 (= M237), E249 (= E243), R250 (= R244), G369 (= G363), A370 (= A364), G371 (= G365), V375 (≠ I369)
- binding flavin-adenine dinucleotide: L128 (= L124), M130 (= M126), S131 (= S127), G136 (= G132), S137 (= S133), W161 (= W157), T163 (= T159), R275 (= R269), F278 (= F272), F285 (= F279), M288 (= M282), Q343 (= Q337), C344 (≠ A338), G347 (= G341), T372 (= T366), E374 (= E368)
8sgrA Human liver mitochondrial isovaleryl-coa dehydrogenase (see paper)
61% identity, 98% coverage: 6:380/381 of query aligns to 12:390/393 of 8sgrA
- binding flavin-adenine dinucleotide: S135 (= S127), G140 (= G132), S141 (= S133), W165 (= W157), T167 (= T159), R279 (= R269), F282 (= F272), I286 (= I276), F289 (= F279), Q347 (= Q337), C348 (≠ A338), G351 (= G341), L369 (= L359), G375 (= G365), T376 (= T366), L382 (≠ Y372)
P26440 Isovaleryl-CoA dehydrogenase, mitochondrial; IVD; Butyryl-CoA dehydrogenase; EC 1.3.8.4; EC 1.3.8.1 from Homo sapiens (Human) (see 5 papers)
61% identity, 98% coverage: 6:380/381 of query aligns to 45:423/426 of P26440
- 165:174 (vs. 124:133, 80% identical) binding FAD
- S174 (= S133) binding substrate
- WIT 198:200 (= WIT 157:159) binding FAD
- SR 222:223 (≠ -K 180) binding substrate
- G250 (= G207) to A: in IVA; uncertain significance
- Y277 (≠ K234) binding substrate
- DLER 284:287 (≠ DYER 241:244) binding substrate
- E286 (= E243) active site, Proton acceptor; mutation to D: Residual isovaleryl-CoA dehydrogenase activity.; mutation to G: Loss of isovaleryl-CoA dehydrogenase activity. Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 407-E.; mutation to Q: Loss of isovaleryl-CoA dehydrogenase activity.
- A291 (= A248) to V: in IVA; uncertain significance; dbSNP:rs886042098
- R312 (= R269) binding FAD
- Q323 (= Q280) binding FAD
- I379 (= I336) to T: in IVA; uncertain significance
- QCFGG 380:384 (≠ QALGG 337:341) binding FAD
- R398 (= R355) to Q: in IVA; uncertain significance; dbSNP:rs1477527791
- Y403 (= Y360) to N: in IVA; uncertain significance
- A407 (= A364) mutation to E: Does not affect isovaleryl-CoA dehydrogenase activity; when associated with 286-D.
- AG 407:408 (= AG 364:365) binding substrate
- TSE 409:411 (≠ TNE 366:368) binding FAD
Sites not aligning to the query:
- 1:32 modified: transit peptide, Mitochondrion
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
43% identity, 96% coverage: 13:379/381 of query aligns to 11:377/378 of 5ol2F
- active site: L124 (≠ M126), T125 (≠ S127), G241 (≠ E243), G374 (≠ R376)
- binding calcium ion: E29 (= E31), E33 (≠ S35), R35 (≠ T37)
- binding coenzyme a persulfide: L238 (= L240), R242 (= R244), E362 (≠ A364), G363 (= G365)
- binding flavin-adenine dinucleotide: F122 (≠ L124), L124 (≠ M126), T125 (≠ S127), P127 (≠ V129), T131 (≠ S133), F155 (≠ W157), I156 (= I158), T157 (= T159), E198 (≠ L200), R267 (= R269), F270 (= F272), L274 (≠ I276), F277 (= F279), Q335 (= Q337), L336 (≠ A338), G338 (= G340), G339 (= G341), Y361 (≠ G363), T364 (= T366), E366 (= E368)
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
42% identity, 99% coverage: 3:379/381 of query aligns to 2:378/380 of 4l1fA
- active site: L125 (≠ M126), T126 (≠ S127), G242 (≠ E243), E363 (≠ A364), R375 (= R376)
- binding coenzyme a persulfide: T132 (≠ S133), H179 (≠ K180), F232 (≠ V233), M236 (= M237), E237 (≠ S238), L239 (= L240), D240 (= D241), R243 (= R244), Y362 (≠ G363), E363 (≠ A364), G364 (= G365), R375 (= R376)
- binding flavin-adenine dinucleotide: F123 (≠ L124), L125 (≠ M126), T126 (≠ S127), G131 (= G132), T132 (≠ S133), F156 (≠ W157), I157 (= I158), T158 (= T159), R268 (= R269), Q270 (= Q271), F271 (= F272), I275 (= I276), F278 (= F279), L281 (≠ M282), Q336 (= Q337), I337 (≠ A338), G340 (= G341), I358 (≠ L359), Y362 (≠ G363), T365 (= T366), Q367 (≠ E368)
- binding 1,3-propandiol: L5 (= L6), Q10 (≠ D11)
8sgsA Human liver mitochondrial short-chain specific acyl-coa dehydrogenase (see paper)
43% identity, 99% coverage: 6:381/381 of query aligns to 4:379/381 of 8sgsA
- binding coenzyme a: S131 (= S133), A133 (≠ V135), N177 (≠ P179), F231 (≠ V233), M235 (= M237), L238 (= L240), I312 (≠ R314), E362 (≠ A364), G363 (= G365)
- binding flavin-adenine dinucleotide: F122 (≠ L124), L124 (≠ M126), S125 (= S127), G130 (= G132), S131 (= S133), W155 (= W157), T157 (= T159), R267 (= R269), F270 (= F272), L274 (≠ I276), L277 (≠ F279), Q335 (= Q337), I336 (≠ A338), G338 (= G340), G339 (= G341), I357 (≠ L359), I360 (= I362), Y361 (≠ G363), T364 (= T366), E366 (= E368)
7y0aC Crystal structure of human short-chain acyl-coa dehydrogenase
43% identity, 99% coverage: 6:381/381 of query aligns to 10:385/387 of 7y0aC
- binding flavin-adenine dinucleotide: F128 (≠ L124), L130 (≠ M126), S131 (= S127), G136 (= G132), S137 (= S133), W161 (= W157), T163 (= T159), T214 (= T210), R273 (= R269), F276 (= F272), L280 (≠ I276), L283 (≠ F279), V285 (≠ L281), Q341 (= Q337), I342 (≠ A338), G345 (= G341), I363 (≠ L359), Y367 (≠ G363), T370 (= T366), E372 (= E368), L376 (≠ Y372)
7y0bA Crystal structure of human short-chain acyl-coa dehydrogenase
43% identity, 99% coverage: 6:381/381 of query aligns to 7:382/385 of 7y0bA
- binding (2S,3R,4R,5S,6R)-2-[4-chloranyl-3-[[4-[(3S)-oxolan-3-yl]oxyphenyl]methyl]phenyl]-6-(hydroxymethyl)oxane-3,4,5-triol: M343 (≠ A342), T347 (≠ K346), E348 (= E347)
- binding flavin-adenine dinucleotide: F125 (≠ L124), L127 (≠ M126), S128 (= S127), G133 (= G132), S134 (= S133), W158 (= W157), T160 (= T159), R270 (= R269), F273 (= F272), L280 (≠ F279), V282 (≠ L281), Q338 (= Q337), I339 (≠ A338), G342 (= G341), I360 (≠ L359), Y364 (≠ G363), T367 (= T366), E369 (= E368), I370 (= I369), L373 (≠ Y372)
P16219 Short-chain specific acyl-CoA dehydrogenase, mitochondrial; SCAD; Butyryl-CoA dehydrogenase; EC 1.3.8.1 from Homo sapiens (Human) (see 3 papers)
43% identity, 99% coverage: 6:381/381 of query aligns to 34:409/412 of P16219
- G90 (= G62) to S: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908005
- E104 (= E76) natural variant: Missing (in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs387906308)
- 152:161 (vs. 124:133, 60% identical) binding in other chain
- R171 (≠ E143) to W: 69% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1800556
- WIT 185:187 (= WIT 157:159) binding in other chain
- A192 (= A164) to V: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940874
- G209 (= G181) to S: 86% of wild-type acyl-CoA dehydrogenase activity; confers susceptibility to ethylmalonicaciduria; dbSNP:rs1799958
- R297 (= R269) binding FAD
- Q308 (= Q280) binding in other chain
- R325 (= R297) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs121908006
- S353 (= S325) to L: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28941773
- QILGG 365:369 (≠ QALGG 337:341) binding FAD
- R380 (= R352) to W: in ACADSD; loss of acyl-CoA dehydrogenase activity; dbSNP:rs28940875
- TSE 394:396 (≠ TNE 366:368) binding in other chain
Sites not aligning to the query:
- 1:24 modified: transit peptide, Mitochondrion
1jqiA Crystal structure of rat short chain acyl-coa dehydrogenase complexed with acetoacetyl-coa (see paper)
42% identity, 99% coverage: 6:381/381 of query aligns to 7:382/384 of 1jqiA