SitesBLAST

R26 (= R26) mutation R->G,S,Q: Strong decrease in phenylalanine transport activity.
P54 (= P54) mutation to A: 50% of wild-type phenylalanine transport activity.; mutation to G: No change in phenylalanine transport activity.; mutation to L: 26% of wild-type phenylalanine transport activity.
F87 (= F87) mutation to L: No effect on phenylalanine transport activity.
F90 (= F90) mutation to L: 65% of wild-type phenylalanine transport activity.
Y92 (≠ G92) mutation to L: 41% of wild-type phenylalanine transport activity.
Y94 (= Y94) mutation to L: 69% of wild-type phenylalanine transport activity.
W95 (≠ L95) mutation to L: 10% of wild-type phenylalanine transport activity.
F98 (≠ C98) mutation to L: No effect on phenylalanine transport activity.
F101 (= F101) mutation to L: 38% of wild-type phenylalanine transport activity.
W105 (= W105) mutation to L: 39% of wild-type phenylalanine transport activity.
Y107 (= Y107) mutation to L: No effect on phenylalanine transport activity.
W108 (= W108) mutation to L: 71% of wild-type phenylalanine transport activity.
F111 (≠ W111) mutation to L: 60% of wild-type phenylalanine transport activity.; mutation to Y: Enables the transport of tryptophan to almost the same steady-state level as that of phenylalanine.
E118 (= E118) mutation E->G,L,V,N: Loss of activity.
K168 (= K168) mutation K->L,R: Strong decrease in phenylalanine transport activity.; mutation to N: Loss of activity.
E226 (= E229) mutation E->A,Q,K,R,W: Loss of activity.
R252 (= R255) mutation R->D,E,F,W,P: Loss of activity.
P341 (= P344) mutation to A: 5% of wild-type phenylalanine transport activity.; mutation P->G,Q,K,R: Loss of activity.; mutation to S: 3% of wild-type phenylalanine transport activity.; mutation to T: 17% of wild-type phenylalanine transport activity.
P442 (= P447) mutation to A: 46% of wild-type phenylalanine transport activity.; mutation to G: 52% of wild-type phenylalanine transport activity.; mutation to L: 43% of wild-type phenylalanine transport activity.

Q88CZ8 L-histidine transporter HutT from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
42% identity, 95% coverage: 14:457/467 of query aligns to 2:445/467 of Q88CZ8

query
sites
Q88CZ8

E

Q

E

A

P

Q

H

G

L

L

A

K

R

R

N

G

L

L

S

S

N

A

R

R

H

H

L

I

Q

R

L

F

I

M

A

A

I

L

G

G

G

S

T

A

I

I

G

G

T
|
T

G

G

L

L

F

F

M

Y

G

G

S

S

G

A

K

S

A

A

V

I

S

Q

L

M

A

A

G

G

P

P

S

A

I

V

L

L

I

A

Y

Y

A

L

I

I

T

G

G

G

F

A

M

A

L

V

F

F

F

M

V

V

M

M

R

R

A

A

L

L

G

G

E

E

I

M

L

A

L

V

S

H

N

N

L

P

Q

V

Y

A

R

G

S

S

F

F

A

G

D

H

F

Y

A

A

G

T

D

T

Y

Y

L

L

G

G

P

P

C

M

A

A

Q

G

F

F

F

I

T

L

G

G

W

W

T

T

Y

Y

W

A

L

F

C
x
E

W

M

I

V

V

I

T

V

A

A

V

I

A

A

E

D

V

V

V

T

A

A

V

F

S

G

G

I

Y

Y

V

M

S

G

F

F

W

W

F

F

P

P

H

E

L

V

A

A

P

R

W

W

I

I

P

W

A

V

L

L

G

G

L

I

I

V

T

F

I

L

L

I

L

G

I

G

L

L

N

N

L

L

P

C

T

N

V

V

R

K

N

V

F

F

G

G

E

E

I

M

E

E

F

F

W

W

F

L

A

S

L

L

I

L

K
|
K

I

V

I

G

T

A

I

I

I

V

G

A

L

M

I

I

I

L

T

A

G

G

I

L

Y

G

M

I

L

M

M

A

T

F

G

G

F

F

V

S

-

Q

L

V

P

G

N

T

G

G

T

H

Q

A

A

V

S

G

I

M

A

S

H

N

L

L

W

F

N

D

H

H

G

G

F

F

F

M

P

P

N

N

G

G

S

V

L

G

G

G

F

L

I

I

A

A

G

S

F

F

Q

A

I

V

S

V

V

M

F
|
F

A

A

F

F

V

G

G

G

I

I

E
|
E

L

I

V

I

G

G

T

V

A

T

A

A

A

G

E

E

A

A

E

K

N

D

P

P

M

Q

R

R

N

V

L

I

P

P

K

K

A

A

I

I

N

N

N

A

I

V

P

P

I

L

R

R

I

I

V

L

L

L

F

F

Y

Y

I

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G

L

A

T

L

L

F

F

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L

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M

T

C

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L

T

Y

P

P

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W

N

P

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D

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N

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G

S

S

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P

F

F

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I

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S

L

N

A

L

G

G

I

I

G

G

I

S

A

A

H

A

F

V

I

L

N

N

F

V

V

V

L

I

T

S

S

A

A

A

S

I

S

S

S

A

S

I

N

N

S

S

G

D

I

I

Y

F

S

G

T

A

S

G

R

R

M

M

V

M

Y

Y

G

G

L

L

A

A

T

Q

V

Q

G

G

L

H

A

A

P

P

K

R

A

G

F

F

S

S

K

K

L

L

S

S

N

K

R

H

K

G

V

V

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P

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W

H

M

A

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L

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F

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M

C

G

I

A

F

A

L

L

S

I

S

G

V

V

V

L

L

L

L

N

Y

Y

A

L

G

I

Q

P

S

E

M

-

I

-

Q

N

V

V

F

F

T

L

L

L

V

I

T

A

T

S

I

I

S

A

A

T

L

F

L

A

F

T

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F

W

I

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W

W

S

L

I

M

I

I

L

L

V

L

S

T

Y

Q

L

V

Q

A

Y

M

R

R

K

S

-

M

H

S

L

R

E

E

R

Q

H

V

E

A

K

Q

S

L

T

K

F

F

K

P

M

V

P

P

G

F

G

W

R

P

A

Y

S

G

V

P

V

A

M

M

V

A

F

I

I

A

F

F

F

M

A

V

F

F

V

I

L

F

W

G

A

V

L

L

T

G

Q

Y

E

F

P

P

D

D

T

T

L

Q

A

A

M

L

K

I

V

V

T

G

P

V

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I

W

W

F

V

V

V

L

F

L

L

G

V

I

A

A

S

Y

Y

T27 (= T39) mutation T->A,S: Retains 60% of wild-type activity.; mutation to N: Retains 20% of wild-type activity.
E98 (≠ C110) mutation to A: Retains 80% of wild-type activity.
K156 (= K168) mutation K->A,Q: Retains less than 10% of wild-type activity.; mutation to R: Retains 40% of wild-type activity.
F212 (= F223) mutation F->A,Q: Loss of activity.; mutation to Y: No change in activity.
E218 (= E229) mutation E->A,Q: Loss of activity.; mutation to D: Retains 70% of wild-type activity.

P15993 Aromatic amino acid transport protein AroP; Aromatic amino acid:H(+) symporter AroP; General aromatic amino acid permease; General aromatic transport system from Escherichia coli (strain K12) (see paper)
39% identity, 95% coverage: 13:457/467 of query aligns to 5:444/457 of P15993

query
sites
P15993

R

Q

E

Q

E

H

E

G

P

E

H

Q

L

L

A

K

R

R

N

G

L

L

S

K

N

N

R

R

H

H

L

I

Q

Q

L

L

I

I

A

A

I

L

G

G

T

A

I

I

G

G

T

T

G

G

L

L

F

F

M

L

G

G

S

S

G

A

K

S

A

V

V

I

S

Q

L

S

A

A

G

G

P

P

S

G

I

I

L

I

L

L

I

G

Y

Y

A

A

I

I

T

A

G

G

F

F

M

I

L

A

F

F

F

L

V

I

M

M

R

R

A

Q

L

L

G

G

E

E

I

M

L

V

S

E

N

E

L

P

Q

V

Y

A

R

G

S

S

F

F

A

S

D

H

F

F

A

A

G

Y

D

K

Y

Y

L

W

G

G

P

S

C

F

A

A

Q

G

F

F

F

A

T

S

G

G

W

W

T

N

Y

Y

W

W

L

V

C

L

W
x
Y

I

V

V

L

T

V

A

A

V

M

A

A

E

E

V

L

V

T

A

A

V

V

S

G

G

K

Y

Y

V

I

S

Q

F

F

W

W

F

Y

P

P

H

E

L

I

A

P

P

T

W

W

I

V

P

S

A

A

L

A

G

V

L

F

I

F

T

V

I

V

L

I

L

N

I

A

L

I

N

N

L

L

P

T

T

N

V

V

R

K

N

V

F

F

G

G

E

E

I

M

E

E

F

F

W

W

F

F

A

A

L

I

I

I

K

K

I

V

I

I

T

A

I

V

G

A

L

M

I

I

T

F

G

G

I

G

Y

W

M

L

L

L

M

F

T

S

G

G

F

-

V

-

L

-

P

N

N

G

G

G

T

P

Q

Q

A

A

S

T

I

V

A

S

H

N

L

L

W

W

N

D

H

Q

G

G

F

F

F

L

P

P

N

H

G

G

S

F

L

T

G

G

F

L

I

V

A

M

G

M

F

M

Q

A

I

I

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I

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M

F

F

A

S

F

F

V

G

G

G

I

L

E

E

L

L

V

V

G

G

T

I

A

T

A

A

E

E

A

A

E

D

N

N

P

P

M

E

R

Q

N

S

L

I

P

P

K

K

A

A

I

T

N

N

N

Q

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Y

R

R

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L

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F

F

Y

Y

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I

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G

A

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L

F

A

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M

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T

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D

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A

N

D

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S

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P

F

F

V

V

A

L

M

I

F

F

S

H

L

E

A

L

G

G

I

D

G

T

I

F

A

V

A

A

H

N

F

A

I

L

N

N

F

I

V

V

L

L

T

T

S

A

A

A

S

L

S

S

S

V

S

Y

N

N

S

S

G

C

I

V

Y

Y

S

C

T

N

S

S

R

R

M

M

V

L

Y

F

G

G

L

L

A

A

T

Q

V

Q

G

G

L

N

A

A

P

P

K

K

A

A

F

L

S

A

K

S

L

V

S

D

N

K

R

R

K

G

V

V

P

P

V

V

H

N

A

T

L

I

I

L

F

V

S

S

C

A

I

L

F

V

L

T

L

A

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L

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C

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V

L

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Y

A

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A

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M

S

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A

Q

F

V

G

F

L

T

L

L

M

V

A

T

L

T

V

I

V

S

S

A

A

L

L

L

-

F

-

I

V

F

I

I

N

W

W

S

A

I

M

I

I

L

S

V

L

S

A

Y

H

L

M

Q

K

Y

F

R

R

K

A

H

K

L

Q

E

E

R

Q

H

G

E

V

K

V

S

T

T

R

F

F

K

P

M

A

-

L

-

Y

P

P

G

L

G

G

R

N

A

W

S

-

V

-

M

I

V

C

F

L

I

L

F

F

F

M

A

A

F

A

V

V

L

L

W

V

A

I

L

M

T

L

Q

M

E

T

P

P

D

G

T

M

L

A

A

I

A

S

M

V

K

Y

V

L

T

I

P

P

L

V

W

W

F

L

V

I

L

V

L

L

G

G

I

I

A

G

Y

Y

Y103 (≠ W111) Key residue for tryptophan transport; mutation to F: Decreases tryptophan transport to less than 50% of wild-type levels and reduces the ability of tryptophan to inhibit phenylalanine transport from 95 to 62%.

P46349 Gamma-aminobutyric acid permease; GABA permease; 4-aminobutyrate permease; Gamma-aminobutyrate permease; Proline transporter GabP from Bacillus subtilis (strain 168) (see paper)
37% identity, 86% coverage: 13:412/467 of query aligns to 2:396/469 of P46349

query
sites
P46349

R

N

E

Q

E

S

E

Q

P

S

H

G

L

L

A

K

R

K

N

E

L

L

S

K

N

T

R

R

H

H

L

M

Q

T

L

M

I

I

A

S

I

I

G

A

G

G

T

V

I

I

G

G

T

A

G

G

L

L

F

F

M

V

G
|
G

S

S

G

G

K

S

A

V

V

I

S

H

L

S

A

T

G
|
G

P

P

S

G

I

A

L

V

I

S

Y

Y

A

A

I

L

T

A

G

G

F

L

M

L

L

V

F

I

F

F

V

I

M

M

R

R

A

M

L

L

G

G

E

E

I

M

L

S

L

A

S

V

N

N

L

P

Q

T

Y

S

R

G

S

S

F

F

A

S

D

Q

F

Y

A

A

G

H

D

D

Y

A

L

I

G

G

P

P

C

W

A

A

Q

G

F

F

F

T

T

I

G

G

W

W

T

L

Y

Y

W

W

L

F

C

F

W

W

I

V

V

I

T

V

A

I

V

A

A

I

E

E

V

A

V

I

A

A

V

G

S

A

G

G

Y

I

V

I

S

Q

F

Y

W

W

F

F

P

H

H

D

L

I

A

P

P

L

W

W

I

L

P

T

A

S

L

L

G

I

L

L

I

T

T

I

I

V

L

L

L

T

I

L

L

T

N

N

L

V

P

Y

T

S

V

V

R

K

N

S

F

F

G

G

E

E

I

F

E

E

F

Y

W

W

F

F

A

S

L

L

I

I

K

K

I

V

T

T

I

I

G

A

L

F

I

L

I

I

T

V

G

G

I

-

Y

F

M

A

L

F

M

I

T

F

G

G

F

F

V

A

L

-

P

P

N

G

G

S

T

E

Q

P

A

V

S

G

I

F

A

S

H

N

L

L

W

T

N

G

H

K

G

G

F

F

P

P

N

E

G

G

S

I

L

S

G

S

F

V

I

L

A

L

G

G

F

I

Q

V

I

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V

V

I

F

F

A

S

F

F

V

M

G

G

I

T

E

E

L

I

V

V

G

A

T

I

A

A

A

G

E

E

A

T

E

S

N

N

P

P

M

I

R

E

N

S

L

V

P

T

K

K

A

A

I

T

N

R

N

S

I

V

P

V

I

W

R

R

I

I

V

I

L

V

F

F

Y

Y

I

V

G

G

A

S

L

I

F

A

V

I

I

V

T

A

V

L

T

L

P

P

W

W

N

N

Q

S

V

A

D

N

P

I

N

L

S

E

S

S

P

P

F

F

V

V

A

A

M

V

F

L

S

E

L

H

A

I

G

G

I

V

G

P

I

A

A

A

H

Q

F

I

I

M

N

N

F

F

V

I

V

V

L

L

T

T

S

A

A

V

S

L

S

S

S

C

S

L

N

N

S

S

G
|
G

I

L

Y

Y

S

T

T

T

S

S

R

R

M

M

V

L

Y

Y

G

S

L

L

A

A

T

E

V

R

G

N

L

E

A

A

P

P

K

R

A

R

F

F

S

M

K

K

L

L

S

S

N

K

R

K

K

G

V

V

P

P

V

V

H

Q

A

A

L

I

I

V

F

A

S
x
G

C

T

I

F

F
|
F

L

S

L

Y

S

I

S

A

V

V

L

M

L

N

Y

Y

A

F

G

S

Q

P

S

D

M

-

I

-

Q

T

V

V

F

F

T

L

L

F

V

L

T

V

T

N

I

S

S

S

A

G

L

A

L

I

F

A

I

L

F

L

I

V

W

Y

S

L

I

V

I

I

L

A

V

V

S

S

Y

Q

L

L

Q

K

Y

M

R

R

R

K

K

K

H

-

L

L

E

E

R

K

H

T

E

N

K

P

S

E

T

A

F

L

K

K

M

I

G33 (= G44) mutation to D: Lack of activity.
G42 (= G53) mutation to S: Lack of activity.
G301 (= G314) mutation to V: Lack of activity.
G338 (≠ S351) mutation to E: Lack of activity.
F341 (= F354) mutation to S: Lack of activity.

Sites not aligning to the query:

414 G→R: Lack of activity.

P25737 Lysine-specific permease LysP; Lysine transporter LysP; Trigger transporter LysP from Escherichia coli (strain K12) (see 2 papers)
36% identity, 94% coverage: 10:449/467 of query aligns to 4:447/489 of P25737

query
sites
P25737

D

E

L

T

H

K

R

T

E

T

E

E

E

A

P

P

H

G

L

L

A

R

R

R

N

E

L

L

S

K

N

A

R

R

H

H

L

L

Q

T

L

M

I

I

A

A

I

I

G

G

T

S

I

I

G

G

T

T

G

G

L

L

F

F

M

V

G

A

S

S

G

G

K

A

A

T

V

I

S

S

L

Q

A

A

G

G

P

P

S

G

-

G

I

A

L

L

I

S

Y

Y

A

M

I

L

T

I

G

G

F

L

M

M

L

V

F

Y

F

F

V

L

M

M

R

T

A

S

L

L

G

G

E

E

I

L

L

A

S

Y

N

M

L

P

Q

V

Y

S

R

G

S

S

F

F

A

A

D

T

F

Y

A

G

G

Q

D

N

Y

Y

L

V

G

E

P

E

C

G

A

F

Q

G

F

F

F

A

T

L

G

G

W

W

T

N

Y
|
Y

W

W

L

Y

C

N

W
|
W

I

A

V

V

T

T

A

I

V

A

A

V

E

D

V

L

V

V

A

A

V

A

S

Q

G

L

Y

V

V

M

S

S

F

W

W

W

F

F

P

P

H

D

L

T

A

P

P

G

W

W

I

I

P

W

A

S

L

A

G

L

L

F

I

L

T

G

I

V

L

I

L

F

I

L

L

L

N

N

L

Y

P

I

T

S

V

V

R

R

N

G

F

F

G

G

E

E

I

A

E

E

F

Y

W

W

F

F

A

S

L

L

I

I

K
|
K

I

V

I

T

T

T

I

V

I

I

G

V

L

F

I

I

T

V

G

G

I

V

Y

L

M

M

L

I

M

I

T

G

G

I

F

F

V

-

L

-

P

-

N

K

G

G

T

A

Q

Q

A

P

S

A

I

G

A

W

H

S

L

N

W

W

N

T

H

I

G

G

-

E

G

A

F

P

F

F

P

A

N

G

G

G

S

F

L

A

G

A

F

M

I

I

A

G

G

V

F

A

Q

M

I

I

S

V

V

G

F
|
F

A

S

F

F

V

Q

G

G

I

T

E
|
E

L

L

V

I

G

G

T

I

A

A

A

G

E
|
E

A

S

E

E

N

D

P

P

M

A

R

K

N

N

L

I

P

P

K

R

A

A

I

V

N

R

N

Q

I

V

P

F

I

W

R

R

I

I

V

L

L

L

F

F

Y

Y

I

V

G

F

A

A

L

I

F

L

V

I

I

I

I

S

T

L

V

I

T

I

P

P

W

Y

-

T

-

D

-

P

-

S

-

L

-

R

N

N

Q
x
D

V

V

-

K

D
|
D

P

I

N

S

S

V

S

S

P

P

F

F

V

T

A

L

M

V

F

F

S

Q

L

H

A

A

G

G

I

L

G

L

I

S

A

A

H

A

F

V

I

M

N

N

F

A

V

V

V

I

L

L

T

T

S

A

A

V

S

L

S

S

S

A

S

G

N

N

S

S

G

G

I

M

Y

Y

S

A

T

S

S

T

R

R

M

M

V

L

Y

Y

G

T

L

L

A

A

T

C

V

D

G

G

L

K

A

A

P

P

K

R

A

I

F

F

S

A

K

K

L

L

S

S

N

R

R

G

K

G

V

V

P

P

V

R

H

N

A

A

L

L

I

Y

F

A

S

T

C

T

I

V

F

I

L

A

-

G

L

L

S

C

S

F

V

L

V

T

L

S

L

M

Y

F

A

G

G

N

Q

Q

S

T

M

-

I

-

Q

-

V

V

F

Y

T

L

L

W

V

L

T

L

T

N

I

T

S

S

A

G

L

M

L

T

F

G

I

F

F

I

I

A

W

W

S

L

I

G

I

I

L

A

V

I

S

S

Y

H

L

Y

Q

R

Y

F

R

R

K

G

H

Y

-

V

L

L

E

Q

R

G

H

H

E

D

K

I

S

N

T

D

F

L

K

P

M

Y

P

R

G

S

G

G

R

F

A

F

S

P

V

L

V

G

M

P

V

I

F

F

I

A

F

F

F

I

A

L

F

C

V

L

L

I

W

I

A

T

L

L

T

G

Q

Q

E

N

P

Y

D
x
E

T

-

L

-

A

A

A

F

M

L

K

K

V
x
D

T

T

P

I

L
x
D

W

W

Y102 (= Y107) mutation to L: Retains 4% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
W106 (= W111) mutation to L: Retains 20% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
K163 (= K168) mutation to A: Retains 24% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
F216 (= F223) mutation to L: Retains 13% of wild-type lysine uptake activity. Increases the capacity to inhibit CadC in the presence of lysine.
E222 (= E229) mutation to A: Abolishes lysine uptake. Strongly inhibits CadC.
E230 (= E237) mutation to V: Abolishes lysine uptake. Shows significant less inhibition of CadC.
D275 (≠ Q275) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-278.
D278 (= D277) Essential for the stimulus-dependent interaction with CadC; mutation to A: Retains 88% of wild-type lysine uptake activity, but can hardly inhibit CadC. Cannot interact with CadC; when associated with A-275.
E438 (≠ D438) mutation to A: Retains 14% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D443 (≠ V445) mutation to A: Retains 11% of wild-type lysine uptake activity. Is unable to inhibit CadC.
D446 (≠ L448) mutation to A: Retains 13% of wild-type lysine uptake activity. Is unable to inhibit CadC.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

P04817 Arginine permease CAN1; Canavanine resistance protein 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
32% identity, 85% coverage: 10:406/467 of query aligns to 75:487/590 of P04817

query
sites
P04817

D

D

L

E

H

G

R

E

E

V

E

Q

E

N

P

A

H

E

L

V

A

K

R

R

N

E

L

L

S

K

N

Q

R

R

H

H

L

I

Q

G

L

M

I

I

A

A

I

L

G

G

T

T

I

I

G

G

T

T

G

G

L

L

F

F

M

I

G

G

S

L

G

S

K

T

A
x
P

V

L

S

T

L

N

A

A

G

G

P

P

-

V

S

G

I

A

L

L

L

I

I

S

Y

Y

A

L

I

F

T

M

G

G

F

S

M

L

L

A

F

Y

F

S

V

V

M

T

R

Q

A

S

L

L

G

G

E

E

I

M

L

A

L

T

S

F

N

I

L
x
P

Q
x
V

Y

T

R

S

S
|
S

F

F

A

T

D

V

F

F

A

S

G

Q

D

R

Y

F

L

L

G

S

P

P

C

A

A

F

Q

G

F

A

T

N

G

G

W

Y

T

M

Y
|
Y

W

W

L

F

C

S

W

W

I

A

V

I

T

T

A

F

V

A

A

L

E

E

V

L

V

S

A

V

V

V

S

G

G

Q

Y

V

V

I

S

Q

F

F

W

W

F

T

P

Y

H

K

-

V

-

P

L

L

A

A

P

A

W

W

I

I

P

S

A

I

L

F

G

W

L

V

I

I

T

-

I

-

L

I

L

T

I

I

L

M

N

N

L

L

P

F

T

P

V

V

R

K

N

Y

F

Y

G

G

E

E

I

F

E

E

F

F

W

W

F

V

A

A

L

S

I

I

K

K

I

V

I

L

T

A

I

I

G

G

L

F

I

L

I

I

T

Y

G

C

I

F

Y

C

M

M

L

V

M

C

-

G

T

A

G

G

F

V

V

T

L

G

P

P

N

V

G

G

T

F

Q

R

-

Y

-

W

-

R

-

N

-

P

-

G

-

A

-

W

-

G

-

P

A

G

S

I

I

I

A

S

H

K

L

D

W

K

N

N

H

E

G

G

G

R

F

F

F

-

P

-

N

-

G

-

S

-

L

L

G

G

F

W

I

V

A

S

G

S

F

L

Q

I

I

N

S

A

V

A

F

F

A

T

F

F

V

Q

G

G

I

T

E

E

L

L

V

V

G

G

T

I

A

T

A

A

A
x
G

E

E

A

A

E

A

N

N

P
|
P

M

R

R

K

N

S

L

V

P

P

K

R

A

A

I

I

N

K

I

V

P

V

I

F

R

R

I

I

V

L

L

T

F

F

Y

Y

I

I

G

G

A

S

L

L

F

L

V

F

I

I

I

G

T

L

V

L

T

V

P

P

W

Y

N

N

Q

-

V

-

D

D

P

P

-

K

-

L

-

T

-

Q

-

S

-
x
T

-
x
S

-
x
Y

-

V

N

S

S

T

S

S

P

P

F

F

V

I

A

I

M

A

F

I

S

E

L

N

A

S

G

G

I

T

G

K

I

V

A

L

A

P

H

H

F

I

I

F

N

N

F

A

V

V

V

I

L

L

T

T

S

T

A

I

S

I

S

S

S

A

N

N

S

S

G

N

I

I

Y

Y

S

V

T

G

S

S

R

R

M

I

V

L

Y

F

G

G

L

L

A

S

T

K

V

N

G

K

L

L

A

A

P

P

K

K

A

F

F

L

S

S

K

R

L

T

S

T

N

K

R

G

K

G

V

V

P

P

V

Y

H

I

A

A

L

V

I

F

F

V

S

T

C

A

I

A

F

F

L

-

S

G

S

A

V

L

V

A

L

Y

L

M

Y

E

A

T

G

S

Q

T

S

G

M

G

I

D

Q

K

V

V

F

F

T

E

L
x
W

V

L

T

L

T

N

I

I

S

T

A

G

L

V

L

A

F

G

I
x
F

F

F

I

A

W

W

S

L

I

F

I

I

L

S

V

I

S

S

Y

H

L

I

Q

R

Y

F

-

M

-

Q

-

A

-

L

R

K

R

Y

K

R

H

G

L

I

E

S

R

R

H

D

E

E

P113 (≠ A48) mutation to L: In CAN1-343; confers citrulline transport activity in GAP1-deleted cells.
P148 (≠ L82) mutation to L: In CAN1-337; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, but not sensitivity to L-aspartic acid alpha-hydroxamate or p-fluoro-L-phenylalanine.
V149 (≠ Q83) mutation to F: In CAN1-315; confers citrulline transport activity in GAP1-deleted cells.
S152 (= S86) mutation to F: In CAN1-342; confers citrulline transport activity in GAP1-deleted cells.
Y173 (= Y107) mutation to D: In CAN1-306; confers citrulline transport activity in GAP1-deleted cells.; mutation to H: In CAN1-327; confers citrulline transport activity in GAP1-deleted cells.
G308 (≠ A236) mutation to A: In CAN1-341; confers citrulline transport activity in GAP1-deleted cells.
P313 (= P241) mutation to S: In CAN1-329; confers citrulline transport activity in GAP1-deleted cells and leads to sensitivity to L-glutamic acid alpha-hydroxamate, alpha-aminoisobutyrate, 3-chloro-L-alanine, L-ethionine, L-allylglycine, and D-histidine, L-aspartic acid alpha-hydroxamate and p-fluoro-L-phenylalanine.
TS 354:355 (vs. gap) mutation Missing: In CAN1-318; confers citrulline transport activity in GAP1-deleted cells.
Y356 (vs. gap) mutation to H: In CAN1-340; confers citrulline transport activity in GAP1-deleted cells.; mutation to N: In CAN1-339; confers citrulline transport activity in GAP1-deleted cells.
W451 (≠ L374) mutation to C: In CAN1-328; confers citrulline transport activity in GAP1-deleted cells.; mutation to L: In CAN1-316; confers citrulline transport activity in GAP1-deleted cells.; mutation to S: In CAN1-335; confers citrulline transport activity in GAP1-deleted cells.
F461 (≠ I384) mutation to S: In CAN1-307; confers citrulline transport activity in GAP1-deleted cells.

P19145 General amino-acid permease GAP1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
32% identity, 85% coverage: 19:415/467 of query aligns to 86:495/602 of P19145

query
sites
P19145

L

L

A

K

R

H

N

H

L

L

S

K

N

N

R

R

H

H

L

L

Q

Q

L

M

I

I

A

A

I

I

G

G

T

A

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

S

G

G

K

T

A

A

V

L

S

R

L

T

A

G

G

G

P

P

S

A

I

S

L

L

I

I

-

G

Y

W

A

G

I

S

T

T

G

G

F

T

M

M

L

I

F

Y

F

A

V

M

M

V

R

M

A

A

L

L

G

G

E

E

I

L

L

A

L

V

S

I

N

F

L

P

Q

I

Y

S

R

G

S

G

F

F

A

T

D

T

F

Y

A

A

G

T

D

R

Y

F

L

I

G

D

P

E

C

S

A

F

Q

G

F

Y

F

A

T

N

G

N

W

F

T

N

Y

Y

W

M

L

L

C

Q

W

W

I

L

V

V

T

V

A

L

V

P

A

L

E

E

V

I

V

V

A

S

V

A

S

S

G

I

Y

T

V

V

S

N

F

F

W

W

-

G

-

T

F

D

P

P

H

K

L

Y

A

R

P

D

W

G

I

F

P

V

A

A

L

L

G

F

L

W

I

L

T

A

I

I

L

-

L

V

I

I

L

I

N

N

L

M

P

F

T

G

V

V

R

K

N

G

F

Y

G

G

E

E

I

A

E

E

F

F

W

V

F

F

A

S

L

F

I

I

K

K

I

V

I

I

T

T

I

V

G

G

L

F

I

I

T

L

G

G

I

I

Y

I

M

L

L

N

M

C

T

G

G

G

F

G

V

P

L

T

P

-

N

-

G

-

T

G

Q

G

A

Y

S

I

I

G

A

G

H

K

L

Y

W

W

N

H

H

D

G

P

G

G

F

A

F

F

P

A

N

G

G

D

S

T

-

P

-

G

-

A

-

K

-

F

L

K

G

G

F

V

I

C

A

S

G

V

F

F

Q

V

I

T

S

A

V

A

F

F

A

S

F

F

V
x
A

G

G

I

S

E

E

L

L

V

V

G

G

T

L

A

A

A

S

E

E

A

S

E

V

N

E

P

P

M

R

R

K

N

S

L

V

P

P

K

K

A

A

I

A

N

K

N

Q

I

V

P

F

I

W

R

R

I

I

V

T

L

L

F

F

Y

Y

I

I

G

L

A

S

L

L

F

L

V

M

I

I

I

G

T

L

V

L

T

V

P

P

W

Y

N

N

Q

D

-

K

-

S

-

L

-

I

-

G

-

A

-

S

V

V

D

D

P

A

N

A

S

A

S

S

P

P

F

F

V

V

A

I

M

A

F

I

S

K

L

T

A

H

G

G

I

I

G

K

I

G

A

L

A

P

H

S

F

V

I

V

N

N

F

V

V

V

V

I

L

L

T

I

S

A

A

V

S

L

S

S

S

V

S

G

N

N

S

S

G

A

I

I

Y

Y

S

A

T

C

S

S

R

R

M

T

V

M

Y

V

G

A

L

L

A

A

T

E

V

Q

G

R

L

F

A

L

P

P

K

E

A

I

F

F

S

S

K

Y

L

V

S

D

N

R

R

K

K

G

V

R

P

P

V

L

H

V

A

G

L

I

I

A

F

V

S

T

C

S

I

A

F

F

L

G

L

L

S

-

V

I

V

A

L

F

L

V

Y

A

A

A

G

S

Q

K

S

K

M

E

I

G

Q

E

V

V

F

F

T

N

L

W

V

L

T

L

T

A

I

L

S

S

A

G

L

L

L

S

F

S

I

L

F

F

I

T

W

W

S

G

I

G

I

I

L

C

V

I

S

C

Y

H

L

I

Q

R

Y

F

R

R

R

K

K

A

H

L

L

A

E

A

R

Q

H

G

-

R

-

G

-

L

E

D

K

E

S

L

T

S

F

F

K

K

M

S

P

P

G

T

G

G

A297 (≠ V226) mutation to V: Impairs basic amino-acids transport and regulation by these amino-acids.

Sites not aligning to the query:

76 modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9URZ4 Cationic amino acid transporter 1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
31% identity, 82% coverage: 14:398/467 of query aligns to 75:466/587 of Q9URZ4

query
sites
Q9URZ4

E

E

E

D

E

G

P

T

H

A

L

L

A

K

R

R

N

S

L

L

S

K

N

S

R

R

H

H

L

M

Q

Q

L

M

I

I

A

S

I

I

G

G

T

A

I

I

G

G

T

T

G

G

L

L

F

Y

M

V

G

G

S

S

G

G

K

S

A

S

V

L

S

A

L

D

A

G

G

G

P

P

-

A

S

S

I

V

L

I

I

N

Y

Y

A

S

I

L

T

I

G

G

F

I

M

M

L

M

F

F

V

I

M

V

R

Y

A

A

L

L

G

G

E

E

I

M

L

A

L

V

S

A

N

Y

L

P

Q

V

Y

A

R

G

S

G

F

F

A

N

D

T

F

Y

A

A

G

T

D

R

Y

F

L

I

G

D

P

P

C

A

A

W

Q

G

F

F

F

A

T

V

G

S

W

W

T

N

Y

Y

W

F

L

I

C

N

W

Y

I

F

V

V

T

T

A

F

V

P

A

L

E

E

V

L

V

T

A

T

V

C

S

A

G

I

Y

T

V

F

S

R

F

Y

W

W

F

T

P

D

-

I

H

N

L

S

A

A

P

A

W

W

I

I

P

S

A

I

L

F

G

-

L

-

I

L

T

V

I

V

L

I

I

I

L

V

N

N

L

L

P

F

T

G

V

V

R

R

N

A

F

Y

G

G

E

E

I

V

E

E

F

F

W

I

F

L

A

S

L

T

I

V

K

K

I

V

T

A

I

T

I

F

G

G

L

F

I

I

T

L

G

A

I

I

Y

I

M

I

L

N

M

C

T

G

G

G

F

-

V

-

L

V

P

P

N

T

G

D

T

H

Q

R

A

G

S

Y

I

I

A

G

H

G

L

S

W

I

N

I

H

K

G

H

G

K

F

P

F

F

P

R

N

H

G

G

S

F

L

K

G

G

F

F

I

C

A

S

G

V

F

F

Q

T

I

T

S

A

V

A

F

F

A

S

F

F

V

S

G

G

I

T

E

E

L

V

V

I

G

G

T

L

A

A

E

E

A

V

E

D

N

N

P

P

M

Q

R

K

N

A

L

L

P

P

K

H

A

A

I

V

N

K

N

Q

I

V

P

F

I

W

R

R

I

I

V

A

L

I

F

F

Y

Y

I

V

G

V

A

S

L

L

F

I

V

L

I

I

-

G

I

L

T

L

V

I

T

S

P

P

W

-

N

-

Q

-

V

D

D

D

P

P

N

N

-

L

-

M

-

G

-

N

-

G

-

S

-

T

-

S

S

V

S

S

P

P

F

F

V

V

A

L

M

A

F

I

S

K

L

E

A

A

G

N

I

I

G

K

I

G

A

L

A

P

H

S

F

V

I

F

N

N

F

A

V

V

V

I

L

I

T

I

S

S

A

V

S

V

S

S

S

V

S

T

N

N

S

S

G

S

I

T

Y

Y

S

T

T

A

S

G

R

R

M

T

V

L

Y

H

G

G

L

M

A

A

T

N

V

L

G

K

L

Q

A

A

P

P

K

S

A

F

F

F

S

K

K

Y

L

T

S

D

N

R

R

L

K

G

V

R

P

P

V

L

H

L

A

A

L

M

I

I

F

V

S

V

C

L

I

L

F

F

L

G

L

F

S

F

S

A

V

Y

V

I

-

N

-

E

L

A

L

D

Y

K

A

N

G

G

Q

N

S

D

M

V

I

S

Q

D

-

T

V

V

F

F

T

N

L

W

V

L

T

L

T

A

I

L

S

S

A

G

L

L

L

S

F

N

I

F

F

F

I

T

W

W

S

G

I

S

I

I

L

C

V

L

S

C

Y

H

L

I

Q

I

Y

F

R

R

Sites not aligning to the query:

29 modified: Phosphoserine
30 modified: Phosphoserine
37 modified: Phosphoserine

P48813 High-affinity glutamine permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
27% identity, 84% coverage: 6:399/467 of query aligns to 132:535/663 of P48813

query
sites
P48813

K
|
K

P

Q

S

S

V

P

D

Q

L

E

H

Q

R

E

E

Q

E

K

E

Q

P

E

H

N

L

L

A

K

R

K

N

S

L

I

S

K

N

P

R

R

H

H

L

T

Q

V

L

M

I

M

A

S

I

L

G

G

G

T

T

G

I

I

G

G

T

T

G

G

L

L

F

L

M

V

G

G

S

N

G

S

K

K

A

V

V

L

S

N

L

N

A

A

G

G

P

P

S

G

I

G

L

L

L

I

I

I

-

G

Y

Y

A

A

I

I

T

M

G

G

F

S

M

C

L

V

F

Y

F

C

V

I

M

I

R

Q

A

A

L

C

G

G

E

E

I

L

L

A

L

V

S

I

N

-

L

-

Q

-

Y

-

R

-

S

-

F

Y

A

S

D

D

F

L

A

I

G

G

D

G

Y

F

-

N

-

T

-

Y

-

P

-

L

-

F

-

L

L

V

G

D

P

P

C

A

A

L

Q

G

F

F

F

S

T

V

G

A

W

W

T

L

Y

F

W

C

L

L

C

Q

W

W

I

L

V

C

T

V

A

C

V

P

A

L

E

E

V

L

V

V

A

T

V

A

S

S

G

M

Y

T

V

I

S

K

F

Y

W

W

F

T

P

T

H

S

L

V

A

N

P

P

W

D

I

V

P

F

A

V

L

V

G

I

L

F

I

Y

T

V

I

L

L

I

L

V

I

V

L

I

N

N

L

V

P

F

T

G

V

A

R

K

N

G

F

Y

G

A

E

E

I

A

E

D

F

F

W

F

F

F

A

N

L

C

I

C

K

K

I

I

I

L

T

M

I

I

I

V

G

G

L

F

I

F

I

I

T

L

G

A

I

I

Y

I

M

I

L

D

M

C

T

G

G

G

F

-

V

-

L

-

P

-

N

A

G

G

T

T

Q

D

A

G

S

Y

I

I

-

G

A

S

H

K

L

Y

W

W

N

R

H

D

G

P

G

G

F

A

F

F

P

R

N

G

G

D

S

T

-

P

-

I

-

Q

-

R

-

F

L

K

G

G

F

V

I

V

A

A

G

T

F

F

Q

V

I

T

S

A

V

A

F

F

A

A

F

F

V

G

G

M

I

S

E

E

L

Q

V

L

G

A

T

M

A

T

A

A

A

S

E

E

A

Q

E

S

N

N

P

P

M

R

R

K

N

A

L

I

P

P

K

S

A

A

I

A

N

K

I

M

P

I

I

Y

R

R

I

I

V

L

L

F

F

V

Y

F

I

L

G

A

A

S

L

L

F

T

V

L

I

V

I

G

T

F

V

L

T

V

P

P

W

Y

N

T

Q

S

V

D

D

Q

-

L

-

G

-

A

-

G

-

S

-

A

P

T

N

K

S

A

S

S

P

P

F

Y

V

V

A

I

M

A

F

V

S

S

L

S

A

H

G

G

I

V

G

R

I

V

A

V

A

P

H

H

F

F

I

I

N

N

F

A

V

V

V

I

L

L

T

L

S

S

A

V

S

L

S

S

S

V

S

A

N

N

S

G

G

A

I

F

Y

Y

S

T

T

S

S

S

R

R

M

I

V

L

Y

M

G

S

L

L

A

A

T

K

V

Q

G

G

L

N

A

A

P

P

K

K

A

C

F

F

S

D

K

Y

L

I

S

D

N

R

R

E

K

G

V

R

P

P

V

A

H

A

A

A

L

M

I

L

F

V

S

S

C

A

I

L

F

F

L

G

L

V

S

-

V

I

V

A

L

F

L

C

Y

A

A

S

G

S

Q

K

S

K

M

E

I

E

Q

D

V

V

F

F

T

T

L

W

V

L

T

L

T

A

I

I

S

S

A

G

L

L

L

S

F

Q

I

L

F

F

I

T

W

W

S

I

I

T

I

I

L

C

V

L

S

S

Y

H

L

I

Q

R

Y

F

R

R

K132 (= K6) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q03770 SPS-sensor component SSY1; Amino-acid permease homolog SSY1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
24% identity, 85% coverage: 18:413/467 of query aligns to 276:750/852 of Q03770

query
sites
Q03770

H

H

L

I

A

Q

R

R

N

K

L

L

S

K

N

V

R

R

H

H

L

I

Q

Q

L

M

I

L

A

S

I

I

G

G

G

A

T

C

I

F

G

S

T

V

G

G

L

L

F

F

M

L

G

T

S

S

G

G

K

K

A

A

V

F

S

S

L

I

A

A

G

G

P

P

-

F

S

G

I

T

L

L

I

G

Y

F

A

G

I

L

T

T

G

G

F

S

M

I

L

I

F

L

F

A

V

T

M

M

R

L

A

S

L

F

G

T

E

E

I

L

L

S

L

T

S

L

N

I

L

P

Q

V

Y

S

R

S

S

G

F

F

A

S

D

G

F

L

A

A

G

S

D

R

Y

F

L

V

G

E

P

D

C

A

A

F

Q

G

F

F

F

A

T

L

G

G

W

W

T

T

Y

Y

W

W

L

I

C

S

W

C

I

M

V

L

T

A

A

L

V

P

A

A

E

Q

V

V

V

S

A

S

V

S

S
x
T

G

F

Y

Y

V

L

S

S

F

Y

W

Y

F

N

P

N

-

V

H

N

L

I

A

S

P

K

W

G

I

V

P

T

A

A

L

-

G

G

L

F

I

I

T

T

I

L

L

F

-

S

-

A

-

F

-

S

L

I

I

V

L

V

N

N

L

L

P

L

T

D

V

V

R

S

N

I

F

M

G

G

E

E

I

I

E

V

F

Y

W

V

F

A

A

G

L

I

I

S

K

K

I

V

I

I

T

I

I

A

I

I

G

L

L

M

I

V

I

F

T

T

G

M

I

I

Y

I

M

L

-

N

-

A

-

G

-

H

-

G

-

N

-

D

-

I

-

H

-

E

-

G

-

V

-

G

-

F

-

R

-

Y

-

W

-

D

-

S

-

K

-

S

-

V

-

R

-

N

L

L

M

T

T

Y

G

G

F

L

V

Y

L

R

P

P

N

T

G

F

T

D

Q

L

A

A

S

D

I

A

A

G

H

E

L

G

W

S

N

K

H

K

G

G

G

I

F

S

F

G

P

P

N

K

G

G

S

R

-

F

L

L

G

A

F

T

I

A

A

S

G

V

F

M

Q

L

I

I

S

S

V

T

F

F

A

A

F

F

V

S

G

G

I

V

E

E

L

M

V

T

G

F

T

L

A

A

A

S

A

G

E

E

A

A

E

I

N

N

P

P

M

R

R

K

N

T

L

I

P

P

K

S

A

A

I

T

N

K

N

R

I

T

P

F

I

S

R

I

I

V

V

L

L

I

F

S

Y

Y

I

V

G

F

A

L

L

I

F

F

-

S

-

V

-

G

-

I

-

N

-

I

-

Y

-

S

-

G

-

D

-

P

-

R

-

L

-

S

-

Y

-

F

-

P

-

G

-

I

-

S

-

E

-

K

-

R

-

Y

-

E

V

A

I

I

T

K

V

G

T

T

-

G

-

M

-

D

-

W

-

R

-

L

-

R

-

T

-

N

-

C

-

R

-

G

-

I

P

D

W

Y

N

R

Q

Q

V

I

D

S

P

V

N

G

-

T

-

G

-

Y

S

S

P

P

F

W

V

V

A

V

M

A

F

L

S

Q

L

N

A

F

G

G

I

L

G

C

I

T

A

F

A

A

H

S

F

A

I

F

N

N

F

A

V

I

V

L

L

I

T

F

S

F

A

T

S

A

S

T

S

A

S

G

N

I

S

S

G

S

I

L

Y

F

S

S

T

C

S

S

R

R

M

T

V

L

Y

Y

G

A

L

M

A

S

T

V

V

Q

G

R

L

K

A

A

P

P

K

P

A

V

F

F

S

E

K

I

L

C

S

S

N

K

R

R

K

G

V

V

P

P

V

Y

H

V

A

S

L

V

I

I

F

F

S

S

C

S

I

L

F

F

L

-

S

S

S

V

V

I

V

A

L

Y

L

I

Y

A

A

V

G

D

Q

Q

S

T

M

A

I

I

Q

E

V

N

F

F

T

D

L

V

V

L

T

A

T

N

I

V

S

S

A

S

L

A

L

S

F

T

I

S

F

I

I

I

W

W

S

M

I

G

I

L

L

N

V

L

S

S

Y

F

L

L

Q

R

Y

F

-

Y

-

A

-

L

-

K

-

Q

R

R

R

K

K

D

H

I

L

I

E

S

R

R

H

N

E

D

K

S

S

S

-

Y

T

P

F

Y

K

K

M

S

P

P

T382 (≠ S123) mutation T->H,L: Constitutively active, up-regulates amino acid permease transcription in response to subthreshold concentrations of amino acids.; mutation to K: In SSY1-102; constitutively active, up-regulates amino acid permease transcription in the absence of amino-acids.; mutation to R: Constitutively active, up-regulates amino acid permease transcription in the absence of amino acids.

P30825 High affinity cationic amino acid transporter 1; CAT-1; CAT1; Ecotropic retroviral leukemia receptor homolog; Ecotropic retrovirus receptor homolog; Solute carrier family 7 member 1; System Y+ basic amino acid transporter from Homo sapiens (Human) (see paper)
23% identity, 87% coverage: 4:409/467 of query aligns to 14:446/629 of P30825

query
sites
P30825

I

L

S

R

K

R

P

K

S

V

V

V

D

D

L

C

H

S

R

R

E

E

E

T

P

-

H

R

L

L

A

S

R

R

N

C

L

L

S

N

N

T

R

F

H

D

L

L

Q

V

L

A

I

L

A

G

I

V

G

G

G

S

T

T

I

L

G

G

T

A

G

G

L

V

F

Y

M

V

G

L

S

A

G

G

K

A

-

V

A

A

V

R

S

E

L

N

A

A

G

G

P

P

S

A

I

I

L

V

L

I

I

S

Y

F

A

L

I

I

T

A

G

A

F

L

M

A

L

S

F

V

F

L

V

A

M

G

R

L

A

C

L

Y

G

G

E

E

I

F

L

G

L

A

S

R

N

V

L

P

Q

K

Y

T

R

G

S

S

F

A

A

Y

D

L

F

Y

A

S

G

Y

D

V

Y

T

L

V

G

G

P

E

C

L

A

W

Q

A

F

F

F

I

T

T

G

G

W

W

T

N

Y

L

W

I

L

L

C

S

W

Y

I

I

V

I

T

-

A

-

V

-

A

-

E

-

V

-

A

G

V

T

S

S

G

S

Y

V

V

A

S

R

F

A

W

W

F

S

P

A

H

T

L

F

A

D

P

E

W

L

I

I

-

G

-

R

P

P

A

I

L

G

G

E

L

F

I

S

T

R

I

T

L

H

L

M

I

T

L

L

N

N

L

A

P

P

T

G

V

V

R

-

N

-

F

L

G

A

E

E

I

N

E

P

F

D

W

I

F

F

A

A

L

V

-

I

-

L

-

I

-

L

-

T

-

G

-

L

-

T

-

L

-

G

I

V

K

K

I

E

I

S

T

A

I

M

I

V

G

N

L

K

I

I

I

F

T

T

G

C

I

I

Y

N

M

V

L

L

M

V

T

L

G

G

F

F

V

I

L

M

P

V

N

S

G

G

-

F

T

V

Q

K

A

G

S

S

I

V

A

K

H

N

-

W

-

Q

-

L

-

T

-

E

-

D

-

F

-

G

-
x
N

-

T

-

S

-

G

-

R

-

L

-

C

L

L

W

N

N

N

H

D

-

T

-

K

-

E

-

G

-

K

-

P

-

G

-

V

G

G

F

F

F

M

P

P

N

F

G

G

S

F

L

S

G

G

F

V

I

L

A

S

G

G

F

A

Q

A

I

T

S

C

V

F

F

Y

A

A

F

F

V

V

G

G

I

F

E

D

L

C

V

I

G

A

T

T

A

T

A

G

A

E

E

E

A

V

E

K

N

N

P

P

M

Q

R

K

N

A

L

I

P

P

K

V

A

G

I

I

N

V

N

A

I

S

P

L

I

L

R

I

I

C

V

F

L

I

F

A

Y

Y

I

F

G

G

A

V

L

S

F

A

V

A

I

L

I

T

T

L

V

M

T

M

P

P

W

Y

N

F

Q

C

V

L

D

D

P

-

N

N

S

N

S

S

P

P

F

L

V

P

A

D

M

A

F

F

S

K

L

H

A

V

G

G

I

W

G

E

I

G

A

A

A

K

H

Y

F

A

I

V

N

A

F

V

V

G

V

S

L

L

T

C

S

A

A

L

S

S

S

A

S

S

S

L

N

L

S

G

G

S

I

M

Y

F

S

P

T

M

S

P

R

R

M

V

V

I

Y

Y

G

A

L

M

A

A

T

E

V

D

G

G

L

L

A

L

P

F

K

K

A

F

F

L

S

A

K

N

L

V

S

N

N

D

R

R

-

T

K

K

V

T

P

P

V

I

H

I

A

A

L

T

I

L

F

A

S

S

C

G

I

A

F

-

L

V

L

A

S

A

S

V

V

M

V

A

L

F

L

L

Y

F

A

D

G

L

Q

K

S

D

M

L

I

V

Q

D

V

L

F

M

T

S

L

I

V

G

T

T

T

L

I

L

S

A

A

-

L

-

F

Y

I

S

F

L

I

V

W

A

S

A

I

C

I

V

L

L

V

V

S

L

Y

R

L

Y

Q

Q

Y

P

R

E

R

Q

K

P

H

N

L

L

E

V

R

Y

H

Q

E

M

K

A

S

S

T

T

N226 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

6f34A Crystal structure of a bacterial cationic amino acid transporter (cat) homologue bound to arginine. (see paper)
27% identity, 45% coverage: 202:413/467 of query aligns to 207:408/458 of 6f34A

query
sites
6f34A

N

N

H

W

G

T

G

P

F

F

F

M

P

P

N

Y

G

G

S

F

L

S

G

G

F

V

I

A

A

T

G

G

F

A

Q

A

I

T

S

V

V

F

F
|
F

A
|
A

F

Y

V
x
I

G

G

I

F

E

D

L

A

V

V

G

S

T

T

A

A

A

E

E

E

A

V

E

R

N

N

P

P

M

Q

R

R

N

D

L

M

P

P

K

I

A

G

I

I

N

I

N

V

I

S

P

L

I

L

R

V

I

C

V

T

L

L

F

L

Y

Y

I

I

G

A

A

V

L

S

F

L

V

V

I

L

I

T

T

G

V

I

T

V

P

P

W

Y

N

E

Q

Q

V

L

D

N

P

V

N

K

S

-

S

N

P

P

F

V

V

A

A

F

M

A

F

L

S

N

L

Y

A

I

G

H

I
x
Q

G

D

I

W

A
x
V

A

A

H

G

F

F

I

I

N

S

F

L

V

G

V

A

L

I

T

A

S

G

A

I

S

T

S
x
V

S

L

N

L

S

V

G

S

I

M

Y

Y

S

G

T

Q

S

T

R

R

M

L

V

F

Y

Y

G

A

L

I

A

S

T

R

V

D

G

G

L

L

A

L

P

P

K

K

A

V

F

F

S

A

K

R

L

I

S

S

-

P

N

T

R

R

K

Q

V

V

P

P

V

-

H

-

A

-

L

-

I

-

F

Y

S

V

C

N

I

T

F

W

L

L

L

T

S

G

S

A

V

A

V

V

L

A

L

V

Y

F

A

A

G

G

Q

I

S

I

M

P

I

L

Q

N

V

K

F

L

T

A

L

E

V

L

T

T

T

N

I

I

S

G

A

T

L

-

L

L

F

F

I

A

F

F

I

I

W

T

S

V

I

S

I

I

L

G

V

V

S

L

Y

V

L

L

Q

R

Y

K

R

T

R

Q

K

P

H

D

L

L

E

K

R

R

H

-

E

-

K

-

S

-

T

A

F

F

K

R

M

V

P

P

binding arginine: F228 (= F223), A229 (= A224), I231 (≠ V226), V314 (≠ S310)
binding : Q296 (≠ I292), V299 (≠ A295)

Sites not aligning to the query:

binding arginine: 40, 42, 43, 44, 115, 116, 119
binding cholesterol: 201, 202
binding : 28, 30, 31, 34, 178, 179, 186, 187, 190, 194

5oqtA Crystal structure of a bacterial cationic amino acid transporter (cat) homologue (see paper)
27% identity, 45% coverage: 202:413/467 of query aligns to 205:406/456 of 5oqtA

query
sites
5oqtA

N

N

H

W

G

T

G

P

F

F

F

M

P

P

N

Y

G

G

S

F

L

S

G

G

F

V

I

A

A

T

G

G

F

A

Q

A

I

T

S

V

V

F

F
|
F

A
|
A

F

Y

V
x
I

G

G

I

F

E

D

L

A

V

V

G

S

T

T

A

A

A

E

E

E

A

V

E

R

N

N

P

P

M

Q

R

R

N

D

L

M

P

P

K

I

A

G

I

I

N

I

N

V

I

S

P

L

I

L

R

V

I

C

V

T

L

L

F

L

Y

Y

I

I

G

A

A

V

L

S

F

L

V

V

I

L

I

T

T

G

V

I

T

V

P

P

W

Y

N

E

Q

Q

V

L

D

N

P

V

N

K

S

-

S

N

P

P

F

V

V

A

A

F

M

A

F

L

S

N

L

Y

A

I

G

H

I
x
Q

G

D

I

W

A
x
V

A

A

H

G

F

F

I

I

N

S

F

L

V

G

V

A

L

I

T

A

S

G

A

I

S

T

S

V

S

L

N

L

S

V

G

M

I

M

Y

Y

S

G

T

Q

S

T

R

R

M

L

V

F

Y

Y

G

A

L

I

A

S

T

R

V

D

G

G

L

L

A

L

P

P

K

K

A

V

F

F

S

A

K

R

L

I

S

S

-

P

N

T

R

R

K

Q

V

V

P

P

V

-

H

-

A

-

L

-

I

-

F

Y

S

V

C

N

I

T

F

W

L

L

L

T

S

G

S

A

V

A

V

V

L

A

L

V

Y

F

A

A

G

G

Q

I

S

I

M

P

I

L

Q

N

V

K

F

L

T

A

L

E

V

L

T

T

T

N

I

I

S

G

A

T

L

-

L

L

F

F

I

A

F

F

I

I

W

T

S

V

I

S

I

I

L

G

V

V

S

L

Y

V

L

L

Q

R

Y

K

R

T

R

Q

K

P

H

D

L

L

E

K

R

R

H

-

E

-

K

-

S

-

T

A

F

F

K

R

M

V

P

P

binding alanine: F226 (= F223), A227 (= A224), I229 (≠ V226)
binding : Q294 (≠ I292), V297 (≠ A295)

Sites not aligning to the query:

binding alanine: 38, 40, 41, 42
binding : 24, 26, 28, 29, 32, 176, 177, 184, 188, 192

P60061 Arginine/agmatine antiporter from Escherichia coli (strain K12) (see 3 papers)
22% identity, 74% coverage: 90:433/467 of query aligns to 76:402/445 of P60061

query
sites
P60061

F

Y

A

A

G

R

D

R

Y

C

L

F

G

G

P

P

C

F

A

L

Q

G

F

Y

F

Q

T

T

G

N

W

V

T

L

Y
|
Y

W

W

L

L

-
x
A

C
|
C

W

W

I

I

V

G

T
x
N

A

-

V

I

A

A

E
x
M

V

V

A

I

V

G

S

V

G

G

Y

Y

V

L

S

S

F

Y

W

F

F

F

P

P

H

I

L

L

A

K

-

D

P

P

W

L

I

V

P

L

A

T

L

I

G

T

L

C

I

V

T

V

I

V

L

L

-

W

-

I

-

F

L

V

I

L

L

L

N

N

L

I

P

V

T

G

V

P

R

K

N

M

F

I

G

T

E

R

I

V

E

Q

F

A

W

V

F

A

A

T

L

V

I

L

K

A

I

L

I

I

T

P

I

I

I

V

G

G

L

I

I

A

I

V

T

F

G

G

I

W

Y

F

M

W

L

F

M

-

T

-

G

-

F

-

V

-

L

-

P

-

N

-

G

-

T

R

Q

G

A

E

S

T

I

Y

A

M

H

A

L

A

W

W

N

N

H

V

G

S

G

G

F

L

F

-

P

-

N

-

G

G

S

T

L

F

G

G

F

A

I

I

-

Q

A

S

G

T

F

L

Q

N

I

V

S

T

V

L

F
x
W

A
x
S

F

F

V
x
I

G

G

I

V

E

E

L

S

V

A

G

S

T

V

A

A

A

G

E

V

A

V

E

K

N

N

P

P

M

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

N

G

I

G

P

V

I

L

R

I

I

A

V

A

L

V

F

C

Y

Y

I

V

G

L

A

S

L

T

F

T

V

A

I

I

I

M

T

G

V

M

T

I

P

P

W

N

N

A

Q

A

V

L

D

R

P

V

N

S

S

A

S

S

P

P

F

F

V

G

A

D

M

A

F

A

S

R

L

M

A

A

G

L

I

G

G

D

I

T

A

A

A

G

H

A

F

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

S

L

S

G

S

S

S

L

N

G

S

G

G
x
W

I

T

Y

L

S

L

T

A

S

G

R

Q

M

T

V

A

Y

K

G

A

L

A

A

A

T

D

V

D

G

G

L

L

A

F

P

P

K

P

A

I

F

F

S

A

K

R

L

V

S

N

N

K

R

A

K

G

V

T

P

P

V

V

H

A

A

G

L

L

-

I

-

V

-

G

I

I

F

L

S

M

C

T

I

I

F

F

L

Q

L

L

S

S

V

I

V

-

L

-

Y

-

A

-

G

S

Q

P

S

N

M

A

I

T

Q

K

V

E

F

F

T

G

L

L

V

V

T

S

I

V

S
|
S

A

V

L

I

L

F

F

T

I

L

F

V

I

P

W

Y

S

L

I

Y

I

T

L

C

V

A

S

A

Y

L

L

L

Q

L

Y

L

R

G

R

H

K

G

H

H

L

F

E

G

R

K

H

A

E

R

K

P

S

-

T

-

F

-

K

-

M

-

P

-

G

-

R

-

A

A

S

Y

V

L

V

A

M

V

V

T

F

T

I

I

F

A

F

F

A

L

F

Y

V

C

L

I

W

W

A

A

L

V

Y93 (= Y107) mutation to L: Greatly decreased Arg uptake into liposomes.
A96 (vs. gap) binding agmatine; binding L-arginine
C97 (= C110) binding agmatine
N101 (≠ T114) binding agmatine; mutation to A: Vmax for Arg-Agm exchange 1% of wild-type, KM increases 3-fold.; mutation to D: Nearly wild-type Arg-Agm exchange.
M104 (≠ E118) binding agmatine; mutation to A: 30% decreased affinity for Arg, 50% decreased affinity for Agm.
W202 (≠ F223) binding L-arginine; mutation to L: Halves Arg uptake into liposomes.
S203 (≠ A224) binding agmatine
I205 (≠ V226) binding agmatine; binding L-arginine; mutation to A: About wild-type affinity for Arg and Agm.
W293 (≠ G314) binding agmatine; mutation W->C,H,L: Loss of Arg-Agm exchange.; mutation W->F,Y: Less than 20% Arg-Agm exchange activity. Vmax 15% of wild-type rate.
S357 (= S379) binding L-arginine; mutation to A: 20% decreased affinity for Arg, 40% decrease affinity for Agm.

Sites not aligning to the query:

23 binding agmatine; binding L-arginine
26 binding L-arginine

P60063 Arginine/agmatine antiporter from Escherichia coli O157:H7 (see 3 papers)
22% identity, 74% coverage: 90:433/467 of query aligns to 76:402/445 of P60063

query
sites
P60063

F

Y

A

A

G

R

D

R

Y

C

L

F

G

G

P

P

C

F

A

L

Q

G

F
x
Y

F

Q

T

T

G

N

W

V

T

L

Y
|
Y

W

W

L

L

-
x
A

C
|
C

W

W

I

I

V

G

T
x
N

A

-

V

I

A

A

E

M

V

V

A

I

V

G

S

V

G

G

Y

Y

V

L

S

S

F

Y

W

F

F

F

P

P

H

I

L

L

A

K

-

D

P

P

W

L

I

V

P

L

A

T

L

I

G

T

L

C

I

V

T

V

I

V

L

L

-

W

-

I

-

F

L

V

I

L

L

L

N

N

L

I

P

V

T

G

V

P

R

K

N

M

F

I

G

T

E

R

I

V

E

Q

F

A

W

V

F

A

A

T

L

V

I

L

K

A

I

L

I

I

T

P

I

I

I

V

G

G

L

I

I

A

I

V

T

F

G

G

I

W

Y

F

M

W

L

F

M

-

T

-

G

-

F

-

V

-

L

-

P

-

N

-

G

-

T

R

Q

G

A

E

S

T

I

Y

A

M

H

A

L

A

W

W

N

N

H

V

G

S

G

G

F

L

F

-

P

-

N

-

G

G

S

T

L

F

G

G

F

A

I

I

-

Q

A

S

G

T

F

L

Q

N

I

V

S

T

V

L

F
x
W

A

S

F

F

V
x
I

G
|
G

I
x
V

E
|
E

L
x
S

V
x
A

G

S

T

V

A

A

A

G

E

V

A

V

E

K

N

N

P

P

M

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

N

G

I

G

P

V

I

L

R

I

I

A

V

A

L

V

F

C

Y

Y

I

V

G

L

A

S

L

T

F

T

V

A

I

I

I

M

T

G

V

M

T

I

P

P

W

N

N

A

Q

A

V

L

D

R

P

V

N

S

S

A

S

S

P

P

F

F

V

G

A

D

M

A

F

A

S

R

L

M

A

A

G

L

I

G

G

D

I

T

A

A

A

G

H

A

F

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

S

L

S

G

S

S

S

L

N

G

S

G

G
x
W

I

T

Y

L

S

L

T

A

S

G

R

Q

M

T

V

A

Y

K

G

A

L

A

A

A

T

D

V

D

G

G

L

L

A

F

P

P

K

P

A

I

F

F

S

A

K

R

L

V

S

N

N

K

R

A

K

G

V

T

P

P

V

V

H

A

A

G

L

L

-

I

-

V

-

G

I

I

F

L

S

M

C

T

I

I

F
|
F

L

Q

L

L

S

S

V

I

V

-

L

-

Y

-

A

-

G

S

Q

P

S

N

M

A

I

T

Q

K

V

E

F

F

T

G

L

L

V

V

T

S

I

V

S
|
S

A

V

L

I

L

F

F

T

I

L

F

V

I

P

W
x
Y

S

L

I

Y

I

T

L

C

V

A

S

A

Y

L

L

L

Q

L

Y

L

R

G

R

H

K

G

H

H

L

F

E

G

R

K

H

A

E

R

K

P

S

-

T

-

F

-

K

-

M

-

P

-

G

-

R

-

A

A

S

Y

V

L

V

A

M

V

V

T

F

T

I

I

F

A

F

F

A

L

F

Y

V

C

L

I

W

W

A

A

L

V

Y87 (≠ F101) mutation to A: Markedly reduced binding affinity for Agm but not for Arg. 50% Agm antiport.
Y93 (= Y107) mutation to A: Reduced binding affinity for Arg, no binding to Agm. 25% Agm antiport.; mutation to K: Almost no binding to both Arg and Agm. 5% Agm antiport.
A96 (vs. gap) binding L-arginine
C97 (= C110) binding L-arginine
N101 (≠ T114) binding L-arginine
W202 (≠ F223) Periplasmic (proximal) gate; binding L-arginine
I205 (≠ V226) binding L-arginine
GVESA 206:210 (≠ GIELV 227:231) Helix-breaking GVESA motif TM6
E208 (= E229) mutation E->A,D: 5-10% Agm antiport.
W293 (≠ G314) binding L-arginine
F337 (= F354) mutation to A: Severely decreased antiport.
S357 (= S379) binding L-arginine
Y365 (≠ W387) mutation to A: Markedly weakened binding to Arg but not to Agm. 5% Agm antiport.

Sites not aligning to the query:

22 N→A: No change in antiport activity, 6-fold higher affinity for Arg.
23 binding L-arginine
25:27 Helix-breaking GSG motif TM1
26 binding L-arginine; S→K: 5% Agm antiport.
27 binding L-arginine
74 Y→A: 50% antiport activity at pH 6.0, 10-fold higher than wild-type antiport activity at pH 7.5, i.e. loss of pH-dependence of substrate transport. No change in binding of Arg or Agm.; mutation Y->C,H,L,M,Q,S: Loss of pH-dependence of substrate transport.; Y→F: Approximately wild-type antiport.

5j4nA Crystal structure of the l-arginine/agmatine antiporter adic in complex with agmatine at 2.6 angstroem resolution (see paper)
22% identity, 74% coverage: 90:433/467 of query aligns to 72:398/437 of 5j4nA

query
sites
5j4nA

F

Y

A

A

G

R

D

R

Y

C

L

F

G

G

P

P

C

F

A

L

Q

G

F

Y

F

Q

T

T

G

N

W

V

T

L

Y

Y

W

W

L

L

-
x
A

C
|
C

W

W

I

I

V
x
G

T
x
N

A

-

V

I

A

A

E
x
M

V

V

A

I

V

G

S

V

G

G

Y

Y

V

L

S

S

F

Y

W

F

F

F

P

P

H

I

L

L

A

K

-

D

P

P

W

L

I

V

P

L

A

T

L

I

G

T

L

C

I

V

T

V

I

V

L

L

-

W

-

I

-

F

L

V

I

L

L

L

N

N

L

I

P

V

T

G

V

P

R

K

N

M

F

I

G

T

E

R

I

V

E

Q

F

A

W

V

F

A

A

T

L

V

I

L

K

A

I

L

I

I

T

P

I

I

I

V

G

G

L

I

I

A

I

V

T

F

G

G

I

W

Y

F

M

W

L

F

M

-

T

-

G

-

F

-

V

-

L

-

P

-

N

-

G

-

T

R

Q

G

A

E

S

T

I

Y

A

M

H

A

L

A

W

W

N

N

H

V

G

S

G

G

F

L

F

-

P

-

N

-

G

G

S

T

L

F

G

G

F

A

I

I

-

Q

A

S

G

T

F

L

Q

N

I

V

S

T

V

L

F

W

A

S

F

F

V

I

G

G

I

V

E

E

L

S

V

A

G

S

T

V

A

A

A

G

E

V

A

V

E

K

N

N

P

P

M

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

N

G

I

G

P

V

I

L

R

I

I

A

V

A

L

V

F

C

Y

Y

I

V

G

L

A

S

L

T

F

T

V

A

I

I

I

M

T

G

V

M

T

I

P

P

W

N

N

A

Q

A

V

L

D

R

P

V

N

S

S

A

S

S

P

P

F

F

V

G

A

D

M

A

F

A

S

R

L

M

A

A

G

L

I

G

G

D

I

T

A

A

A

G

H

A

F

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

S

L

S

G

S

S

S

L

N

G

S

G

G
x
W

I

T

Y

L

S

L

T

A

S

G

R

Q

M

T

V

A

Y

K

G

A

L

A

A

A

T

D

V

D

G

G

L

L

A

F

P

P

K

P

A

I

F

F

S

A

K

R

L

V

S

N

N

K

R

A

K

G

V

T

P

P

V

V

H

A

A

G

L

L

-

I

-

V

-

G

I

I

F

L

S

M

C

T

I

I

F

F

L

Q

L

L

S

S

V

I

V

-

L

-

Y

-

A

-

G

S

Q

P

S

N

M

A

I

T

Q

K

V

E

F

F

T

G

L

L

V

V

T

S

I

V

S

S

A

V

L

I

L

F

F

T

I

L

F

V

I

P

W

Y

S

L

I

Y

I

T

L

C

V

A

S

A

Y

L

L

L

Q

L

Y

L

R

G

R

H

K

G

H

H

L

F

E

G

R

K

H

A

E

R

K

P

S

-

T

-

F

-

K

-

M

-

P

-

G

-

R

-

A

A

S

Y

V

L

V

A

M

V

V

T

F

T

I

I

F

A

F

F

A

L

F

Y

V

C

L

I

W

W

A

A

L

V

binding agmatine: A92 (vs. gap), C93 (= C110), G96 (≠ V113), N97 (≠ T114), M100 (≠ E118), W289 (≠ G314)

Sites not aligning to the query:

binding agmatine: 19

3l1lA Structure of arg-bound escherichia coli adic (see paper)
22% identity, 87% coverage: 28:433/467 of query aligns to 9:385/423 of 3l1lA

query
sites
3l1lA

L

V

Q

T

L

L

I

M

A

V

I

S

G

G

G

A

T
x
I

I

M

G

G

T
x
S

G
|
G

L

V

F

F

M

L

G

L

S

P

G

A

K

N

A

L

V

A

S

S

L

T

A

G

G

G

P

-

S

-

I

-

L

-

I

-

Y

I

A

A

I

I

T

Y

G

G

F

W

M

L

L

V

F

T

F

I

V

I

M

-

R

-

A

-

L

-

G

G

E

A

I

L

L

G

L

L

S

S

N

M

L

V

Q

Y

Y

A

R

K

-

M

S

S

F

F

A

L

D

D

-

P

-

S

-

P

-

G

-

S

-

Y

-

A

F

Y

A

A

G

R

D

R

Y

C

L

F

G

G

P

P

C

F

A

L

Q

G

F

Y

F

Q

T

T

G

N

W

V

T

L

Y

Y

W

W

L

L

-

A

C

C

W

W

I

I

V
x
G

T
x
N

A

-

V

I

A

A

E

M

V

V

A

I

V

G

S

V

G

G

Y

Y

V

L

S

S

F

Y

W

F

F

F

P

P

H

I

L

L

A

K

-

D

P

P

W

W

I

V

P

L

A

T

L

I

G

T

L

C

I

V

T

V

I

V

L

L

-

W

-

I

-

F

L

V

I

L

L

L

N

N

L

I

P

V

T

G

V

P

R

K

N

M

F

I

G

T

E

R

I

V

E

Q

F

A

W

V

F

A

A

T

L

V

I

L

K

A

I

L

I

I

T

P

I

I

I

V

G

G

L

I

I

A

I

V

T

F

G

G

I

W

Y

F

M

-

L

-

M

-

T

-

G

-

F

-

V

-

L

-

P

-

N

-

G

-

T

-

Q

-

A

-

S

-

I

-

A

-

H

-

L

-

W

W

N

F

H

R

G

G

G

E

F

T

F

Y

P

-

N

-

G

-

S

M

L

A

G

A

F

I

I

Q

A

S

G

T

F

L

Q

N

I

V

S

T

V

L

F
x
W

A
x
S

F

F

V
x
I

G

G

I

V

E

E

L

S

V

A

G

S

T

V

A

A

A

G

E

V

A

V

E

K

N

N

P

P

M

K

R

R

N

N

L

V

P

P

K

I

A

A

I

T

N

I

N

G

I

G

P

V

I

L

R

I

I

A

V

A

L

V

F

C

Y

Y

I

V

G

L

A

S

L

T

F

T

V

A

I

I

I

M

T

G

V

M

T

I

P

P

W

N

N

A

Q

A

V

L

D

R

P

V

N

S

S

A

S

S

P

P

F

F

V

G

A

D

M

A

F

A

S

R

L

M

A

A

G

L

I

G

G

D

I

T

A

A

A

G

H

A

F

I

I

V

N

S

F

F

V

C

V

A

L

A

T

A

S

G

A

C

S

L

S

G

S

S

S

L

N

G

S

G

G
x
W

I

T

Y

L

S

L

T

A

S

G

R

Q

M

T

V

A

Y

K

G

A

L

A

A

A

T

D

V

D

G

G

L

L

A

F

P

P

K

P

A

I

F

F

S

A

K

R

L

V

S

N

N

K

R

A

K

G

V

T

P

P

V

V

H

A

A

G

L

L

-

I

-

V

-

G

I

I

F

L

S

M

C

T

I

I

F

F

L

Q

L

L

S

S

V

I

V

-

L

-

Y

-

A

-

G

S

Q

P

S

N

M

A

I

T

Q

K

V

E

F

F

T

G

L

L

V

V

T

S

I

V

S

S

A

V

L

I

L

F

F

T

I

L

F

V

I

P

W

Y

S

L

I

Y

I

T

L

C

V

A

S

A

Y

L

L

L

Q

L

Y

L

R

G

R

H

K

G

H

H

L

F

E

G

R

K

H

A

E

R

K

P

S

-

T

-

F

-

K

-

M

-

P

-

G

-

R

-

A

A

S

Y

V

L

V

A

M

V

V

T

F

T

I

I

F

A

F

F

A

L

F

Y

V

C

L

I

W

W

A

A

L

V

binding arginine: I17 (≠ T36), S20 (≠ T39), G21 (= G40), G94 (≠ V113), N95 (≠ T114), W185 (≠ F223), S186 (≠ A224), I188 (≠ V226), W276 (≠ G314)

P63235 Glutamate/gamma-aminobutyrate antiporter; Glu/GABA antiporter; Extreme acid sensitivity protein from Escherichia coli (strain K12) (see 2 papers)
24% identity, 51% coverage: 186:424/467 of query aligns to 163:417/511 of P63235

query
sites
P63235

G

G

F

I

V

L

L

L

P

P

N

A

G

F

T

I

Q

L

A

I

S

A

I

L

A

A

H

A

L

I

W

Y

N

L

H

H

G

S

G

G

-

A

-

P

-

V

-

A

-

I

-

E

-

M

-

D

-

S

-

K

-

T

F

F

P

P

N

D

G

F

S

S

-

K

L

V

G

G

F

T

I

L

A

V

G

V

F

F

Q

V

I

A

S

F

V

I

F
x
L

A

S

F

Y

V

M

G

G

I

V

E
|
E

L

A

V

S

G

A

T

T

A

H

A

V

A

N

E

E

A

M

E

S

N

N

P

P

M

G

R

R

N

D

L

Y

P

P

K

L

A

A

I

M

N

L

I

L

P

M

I

V

R

A

-

A

I

I

V

C

L

L

F

S

Y

S

I

V

G

G

A

G

L

L

F

-

V

S

I

I

I

A

T

M

V

V

T

I

P

P

W

G

N

N

Q

E

V

I

D

N

P

L

N

S

S

A

-

G

-

V

-

M

S

Q

P

T

F

F

V

T

A

V

M

L

F

M

S

S

L

H

A

V

G

A

I

P

G

E

I

I

-

E

-

W

A

T

A

V

H

R

F

V

I

I

N

S

F

A

V

L

L

L

T

L

S

G

A

V

S

L

S

A

S
x
E

S

I

N

A

S

S

G
x
W

I

I

Y

V

S

G

T

P

S

S

R

R

M

G

V

M

Y

Y

G

V

L

T

A

A

T

Q

V

K

G

N

L

L

A

L

P

P

K

A

A

A

F

F

S

A

K

K

L

M

S

N

N

K

R

N

K

G

V

V

P

P

V

V

H

T

A

L

L

V

I

I

F

S

S

Q

C

L

I

V

F

I

L

T

-

S

L

I

S

A

S

L

V

I

L

L

L

T

Y

N

A

T

G

G

Q

G

S

G

M

N

I

N

Q

M

V

S

F

F

T

L

L

I

V

A

T

L

T

A

I

L

S

T

A

V

L

V

L

I

F
x
Y

I

L

F

C

I

A

W
x
Y

S

F

I

M

I

L

L

F

V

I

S

G

Y

Y

L

I

Q

V

Y

L

R

V

R

L

K

K

H

H

L

P

E

D

R

L

H

-

E

-

K

K

S

R

T

T

F

F

K

N

M

I

P

P

G

G

R

K

A

G

S

V

V

K

V

L

M

V

V

V

F

A

I

I

L212 (≠ F223) mutation to A: 70% decrease in substrate transport.
E218 (= E229) mutation to A: At least 90% decrease in substrate transport.
E304 (≠ S310) mutation to A: At least 90% decrease in substrate transport.
W308 (≠ G314) mutation to A: At least 90% decrease in substrate transport.
Y378 (≠ F383) mutation to A: At least 90% decrease in substrate transport.
Y382 (≠ W387) mutation to A: At least 90% decrease in substrate transport.

Sites not aligning to the query:

25 M→A: 25% decrease in substrate transport.
30 Y→A: At least 90% decrease in substrate transport.
471:511 mutation Missing: Shifts the pH-dependent substrate transport towards higher pH values. Transports Gln, but not Glu, at pH 7.0 or higher.
491 H→A: Allows substrate transport at pH 6.5.
497 R→A: Allows substrate transport at pH 6.5.
499 R→A: Allows substrate transport at pH 6.5.
502 H→A: Allows substrate transport at pH 6.5.
503 Y→A: Allows substrate transport at pH 6.5.

P76037 Putrescine importer PuuP from Escherichia coli (strain K12) (see paper)
24% identity, 38% coverage: 73:249/467 of query aligns to 62:242/461 of P76037

query
sites
P76037

A

A

L

L

G

A

E

G

I

V

L

L

L

F

S

T

N

A

L

I

Q

S

Y

Y

-

G

-

K

-

L

-

V

R

R

S

Q

F

F

A

P

D

E

-

A

-

G

-

S

-

A

-

Y

-

T

F

Y

A

A

G

Q

D

K

Y

S

L

I

G

N

P

P

C

H

A

V

Q

G

F

F

F

M

T

V

G

G

W

W

T

S

Y

S

W

L

L

L

C

D

W
x
Y

I

L

V

F

T

L

A

P

V

M

A

I

E

N

V

V

V

L

A

L

V

A

S

K

G

I

Y

Y

V

L

S

S

F

A

W

L

F

F

P

P

H

E

L

V

A

P

P

P

W

W

I

V

P

W

A

V

L

V

G

T

L

F

I

V

T

A

I

I

L

L

L

T

I

A

L

A

N

N

L

L

P

K

T

S

V

V

R

N

N

L

F

V

G

A

E

N

I

F

E

N

F

T

W

L

F

F

A

V

L

L

I

V

K

Q

I

-

T

-

I

-

I

I

G

S

L

I

I

M

I

V

T

V

G

F

I

I

Y

F

M

L

L

V

M

V

T

Q

G

G

F

L

V

H

L

K

P

G

N

E

G

G

T

-

Q

-

A

-

S

-

I

V

A

G

H

T

L

V

W

W

N

S

H

L

G

Q

G

P

F

F

F

I

P

S

N

E

G

N

S

A

-

H

-

L

L

I

G

P

F

I

I

I

A

T

G

G

F

A

Q

T

I

I

S

V

V

C

F

F

A

S

F

F

V

L

G

G

I

F

E

D

L

A

V

V

G

T

T

T

A

L

A

S

A

E

E

E

A

T

E

P

N

D

P

A

M

A

R

R

N

V

L

I

P

P

K

K

A

A

I

I

Y110 (≠ W111) mutation to X: The uptake activity is reduced to one-eighth of that of wild-type.

P0AAF1 Putrescine transporter PotE; Putrescine-proton symporter / putrescine-ornithine antiporter from Escherichia coli (strain K12) (see 2 papers)
22% identity, 77% coverage: 19:379/467 of query aligns to 1:356/439 of P0AAF1

query
sites
P0AAF1

L

M

A

S

R

Q

N

A

L

K

S

S

N

N

R

K

-

M

-

G

-

V

H

Q

L

L

Q

T

L

I

I

L

A

T

I

M

G

V

G

N

T

M

I

M

G

G

T

S

G

G

L

I

F

I

M

M

G

L

S

P

G

T

K

K

A

L

V

A

S

E

L

V

A

G

G

T

P

I

S

S

I

I

L

I

-

S

-

W

L

L

I

V

Y

T

A

A

I

V

T

G

G

S

F

M

M

A

L

L

F

A

F

W

V

A

M

F

R

A

A

K

L
x
C

G

G

E

M

I

F

L

S

L

R

S
x
K

N

S

L

G

Q

G

Y

M

R

G

S

G

F

Y

A

A

D
x
E

F
x
Y

A

A

G

-

D

-

Y

-

L

F

G

G

P
x
K

C

S

A

G

Q

N

F

F

F

M

T

A

G

N

W
x
Y

T

T

Y
|
Y

W

G

L

V

C

S

W

L

I

L

V

I

T

A

A

N

V

V

A

A

E

I

V

A

V

I

A

S

V

A

S

V

G

G

Y

Y

-

G

V

T

S

E

F

L

W

L

F

G

P

A

H

S

L

L

A

S

P

P

W

V

I

Q

P

I

A

G

L

L

G

A

L

T

I

I

T

G

I

V

L

L

L

W

I

I

L

C

N

-

L

-

P

-

T

T

V

V

R

A

N

N

F

F

G

G

E

G

I

A

E

R

F

I

W

-

F

T

A

G

L

Q

I

I

K

S

I

S

I

I

T

T

I

V

I

W

G

G

L

V

I

I

T

P

G

V

I

V

Y

G

M

L

L

C

M

I

T

I

G

G

F

W

V

F

L

W

P

F

N

S

G

P

T

T

Q

L

A

-

S

-

I

Y

A

V

H

D

L

S

W

W

N

N

-

P

-

H

H

H

G

A

G

P

F

F

P

S

N

-

G

-

S

A

L

V

G

G

F

-

I

-

A

S

G

S

F

I

Q

A

I

M

S

T

V

L

F
x
W

A

A

F

F

V

L

G

G

I

L

E
|
E

L

S

V

A

G
x
C

T

A

A

N

A

T

A

D

E

V

A

V

E

E

N

N

P

P

M

E

R

R

N

N

L

V

P

P

K

I

A

A

I

V

N

L

N

G

I

G

P

T

I

L

R

G

I

A

V

A

L

V

F

I

Y

Y

I

I

G

V

A

S

L

T

F

N

V

V

I

I

I

A

T

G

V

I

T

V

P

P

W

N

N

M

Q

E

V

L

D

A

P

N

N

S

S

T

S

A

P

P

F

F

V

G

A

L

M

A

F

F

S

A

L

Q

A

M

G

F

I

T

G

P

I

E

A

V

A

G

H

K

F

V

I

I

N

M

F

A

V

L

V

M

L

V

T

M

S

S

A
x
C

S
x
C

S

G

S

S

S

L

N

L

S

G

G
x
W

I

Q

Y

F

S

T

T

I

S

A

R

Q

M

V

V

F

Y
x
K

G

S

L

S

A

S

T

D

V

E

G

G

L
x
Y

A

F

P

P

K

K

A

I

F

F

S

S

K

R

L

V

S

T

N

K

R

V

K

D

V

A

P

P

V

V

H

Q

A

G

L

M

I

L

F

-

S

T

C

I

I

V

F

I

L

I

L

Q

S

S

S

G

V

L

V

A

L

L

L

M

Y

T

A

I

G

S

Q

P

S

S

M

L

I

N

Q

S

V

Q

F

F

T

N

L

V

V

L

T

V

T

N

I

L

S

A

C62 (≠ L74) mutation C->A,T: Strong decrease in both uptake and excretion activities.; mutation to S: Moderate decrease in both uptake and excretion activities.
K68 (≠ S80) mutation to A: Slight decrease in both uptake and excretion activities.
E77 (≠ D89) mutation E->A,D,N,Q: Strong decrease in both uptake and excretion activities.
Y78 (≠ F90) mutation to L: Uptake activity decreases more than excretion activity.
K82 (≠ P97) mutation to A: Slight decrease in both uptake and excretion activities.
Y90 (≠ W105) mutation to L: Uptake activity decreases more than excretion activity.
Y92 (= Y107) mutation to L: Moderate decrease in both uptake and excretion activities.
W201 (≠ F223) mutation W->F,L,Y: Strong decrease in both uptake and excretion activities.
E207 (= E229) mutation E->A,D,N,Q: Lack of both uptake and excretion activities.
C210 (≠ G232) mutation to A: Moderate decrease in both uptake and excretion activities.
C285 (≠ A307) mutation to A: Moderate decrease in both uptake and excretion activities.
C286 (≠ S308) mutation to A: Moderate decrease in both uptake and excretion activities.
W292 (≠ G314) mutation W->F,L,Y: Strong decrease in both uptake and excretion activities.
K301 (≠ Y323) mutation to A: Excretion activity decreases more than uptake activity.
Y308 (≠ L330) mutation to L: Excretion activity decreases more than uptake activity.

Sites not aligning to the query:

422 W→L: Uptake activity decreases more than excretion activity.
425 Y→F: Moderate decrease in both uptake and excretion activities.; Y→L: Strong decrease in both uptake and excretion activities.
433 mutation E->A,D,N,Q: Strong decrease in both uptake and excretion activities.

Query Sequence

>WP_012783773.1 NCBI__GCF_000022745.1:WP_012783773.1
MNTISKPSVDLHREEEPHLARNLSNRHLQLIAIGGTIGTGLFMGSGKAVSLAGPSILLIY
AITGFMLFFVMRALGEILLSNLQYRSFADFAGDYLGPCAQFFTGWTYWLCWIVTAVAEVV
AVSGYVSFWFPHLAPWIPALGLITILLILNLPTVRNFGEIEFWFALIKIITIIGLIITGI
YMLMTGFVLPNGTQASIAHLWNHGGFFPNGSLGFIAGFQISVFAFVGIELVGTAAAEAEN
PMRNLPKAINNIPIRIVLFYIGALFVIITVTPWNQVDPNSSPFVAMFSLAGIGIAAHFIN
FVVLTSASSSSNSGIYSTSRMVYGLATVGLAPKAFSKLSNRKVPVHALIFSCIFLLSSVV
LLYAGQSMIQVFTLVTTISALLFIFIWSIILVSYLQYRRKHLERHEKSTFKMPGGRASVV
MVFIFFAFVLWALTQEPDTLAAMKVTPLWFVLLGIAYWVMKLKQKQS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory