SitesBLAST
Comparing WP_012851176.1 NCBI__GCF_000024385.1:WP_012851176.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
2zsjA Crystal structure of threonine synthase from aquifex aeolicus vf5
34% identity, 84% coverage: 59:406/413 of query aligns to 6:343/350 of 2zsjA
- active site: K61 (= K114), T85 (= T138), Q218 (= Q274), A222 (≠ C278), A240 (≠ S303), T317 (≠ S380)
- binding pyridoxal-5'-phosphate: F60 (= F113), K61 (= K114), N87 (= N140), V186 (= V241), G187 (≠ A242), N188 (≠ S243), A189 (≠ G244), G190 (≠ S245), N191 (≠ Q246), A240 (≠ S303), T317 (≠ S380), G318 (= G381)
3aexA Catalytic intermediate analogue of threonine synthase from thermus thermophilus hb8 (see paper)
37% identity, 80% coverage: 56:385/413 of query aligns to 3:322/351 of 3aexA
- active site: K61 (= K114), T85 (= T138), P212 (= P267), G216 (= G272), Q218 (= Q274), A240 (≠ S303), T317 (≠ S380)
- binding (3E)-4-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}-2-oxobut-3-enoic acid: K61 (= K114), S84 (= S137), T85 (= T138), N87 (= N140), T88 (≠ L141), V186 (= V241), G187 (≠ A242), N188 (≠ S243), A189 (≠ G244), G190 (≠ S245), N191 (≠ Q246), A240 (≠ S303), I241 (≠ L304), E287 (= E350), T317 (≠ S380)
- binding phosphate ion: K61 (= K114), T88 (≠ L141), N154 (= N207), S155 (≠ N209), R160 (≠ Y214), N188 (≠ S243)
1v7cA Crystal structure of threonine synthase from thermus thermophilus hb8 in complex with a substrate analogue (see paper)
37% identity, 80% coverage: 56:385/413 of query aligns to 3:322/351 of 1v7cA
- active site: K61 (= K114), T85 (= T138), P212 (= P267), G216 (= G272), Q218 (= Q274), A240 (≠ S303), T317 (≠ S380)
- binding (2e)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-5-phosphonopent-2-enoic acid: K61 (= K114), S84 (= S137), T85 (= T138), N87 (= N140), T88 (≠ L141), F134 (≠ Y186), N154 (= N207), S155 (≠ N209), R160 (≠ Y214), V186 (= V241), G187 (≠ A242), N188 (≠ S243), A189 (≠ G244), G190 (≠ S245), N191 (≠ Q246), A240 (≠ S303), I241 (≠ L304), E287 (= E350), T317 (≠ S380)
1uimA Crystal structure of threonine synthase from thermus thermophilus hb8, orthorhombic crystal form (see paper)
37% identity, 80% coverage: 56:385/413 of query aligns to 3:322/350 of 1uimA
- active site: K61 (= K114), T85 (= T138), P212 (= P267), G216 (= G272), Q218 (= Q274), A240 (≠ S303), T317 (≠ S380)
- binding pyridoxal-5'-phosphate: F60 (= F113), K61 (= K114), N87 (= N140), G187 (≠ A242), N188 (≠ S243), A189 (≠ G244), G190 (≠ S245), N191 (≠ Q246), A240 (≠ S303), E287 (= E350), T317 (≠ S380), G318 (= G381)
3aeyA Apo form of threonine synthase from thermus thermophilus hb8 (see paper)
37% identity, 80% coverage: 56:385/413 of query aligns to 2:321/350 of 3aeyA
- active site: K60 (= K114), T84 (= T138), P211 (= P267), G215 (= G272), Q217 (= Q274), A239 (≠ S303), T316 (≠ S380)
- binding sulfate ion: K60 (= K114), K60 (= K114), G85 (= G139), N86 (= N140), T87 (≠ L141), T87 (≠ L141), S154 (≠ N209), R159 (≠ Y214), N187 (≠ S243), R228 (= R293), V230 (= V295), E231 (vs. gap), R232 (≠ K296), A239 (≠ S303)
6cgqA Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
33% identity, 84% coverage: 59:406/413 of query aligns to 3:331/339 of 6cgqA
- active site: K56 (= K114), T80 (= T138), E206 (≠ G272), S210 (≠ T276), A228 (≠ S303), T305 (≠ S380)
- binding pyridoxal-5'-phosphate: F55 (= F113), K56 (= K114), N82 (= N140), V175 (= V241), G176 (≠ A242), N177 (≠ S243), A178 (≠ G244), G179 (≠ S245), N180 (≠ Q246), A228 (≠ S303), E275 (= E350), T305 (≠ S380), G306 (= G381)
6nmxA Threonine synthase from bacillus subtilis atcc 6633 with plp and appa (see paper)
32% identity, 84% coverage: 59:406/413 of query aligns to 7:341/350 of 6nmxA
- active site: K60 (= K114), T84 (= T138), E216 (≠ Q274), S220 (≠ C278), A238 (≠ S303), T315 (≠ S380)
- binding (2E,3Z)-2-{[(Z)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4(1H)-ylidene}methyl]imino}-5-phosphonopent-3-enoic acid: K60 (= K114), S83 (= S137), T84 (= T138), N86 (= N140), T87 (≠ L141), F133 (≠ Y186), N153 (= N207), S154 (≠ N209), R159 (≠ Y214), V185 (= V241), G186 (≠ A242), N187 (≠ S243), A188 (≠ G244), G189 (≠ S245), N190 (≠ Q246), A238 (≠ S303), I239 (≠ L304), E285 (= E350), T315 (≠ S380)
6cgqB Threonine synthase from bacillus subtilis atcc 6633 with plp and plp- ala (see paper)
32% identity, 84% coverage: 59:406/413 of query aligns to 5:339/345 of 6cgqB
- active site: K58 (= K114), T82 (= T138), E214 (≠ Q274), S218 (≠ C278), A236 (≠ S303), T313 (≠ S380)
- binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-alanine: K58 (= K114), S81 (= S137), T82 (= T138), N84 (= N140), T85 (≠ L141), V183 (= V241), G184 (≠ A242), N185 (≠ S243), A186 (≠ G244), N188 (≠ Q246), A236 (≠ S303), I237 (≠ L304), E283 (= E350), T313 (≠ S380)
- binding phosphate ion: K58 (= K114), T85 (≠ L141), N151 (= N207), S152 (≠ N209), R157 (≠ Y214), N185 (≠ S243)
P9WG59 Threonine synthase; TS; EC 4.2.3.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
33% identity, 81% coverage: 56:388/413 of query aligns to 13:334/360 of P9WG59
- K69 (= K114) modified: N6-(pyridoxal phosphate)lysine
- N95 (= N140) binding pyridoxal 5'-phosphate
- K151 (≠ E195) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
- GNAGN 196:200 (≠ ASGSQ 242:246) binding pyridoxal 5'-phosphate
- T326 (≠ S380) binding pyridoxal 5'-phosphate
2d1fA Structure of mycobacterium tuberculosis threonine synthase (see paper)
33% identity, 81% coverage: 56:388/413 of query aligns to 4:325/349 of 2d1fA
- active site: K60 (= K114), T84 (= T138), D209 (≠ E264), R213 (≠ K269), L215 (≠ F271), A240 (≠ S303), T317 (≠ S380)
- binding pyridoxal-5'-phosphate: F59 (= F113), K60 (= K114), N86 (= N140), V186 (= V241), G187 (≠ A242), N188 (≠ S243), A189 (≠ G244), G190 (≠ S245), N191 (≠ Q246), A240 (≠ S303), T317 (≠ S380)
A0R220 Threonine synthase; TS; EC 4.2.3.1 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
33% identity, 81% coverage: 56:388/413 of query aligns to 13:334/360 of A0R220
- K151 (≠ E195) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
Q9S7B5 Threonine synthase 1, chloroplastic; Protein METHIONINE OVER-ACCUMULATOR 2; EC 4.2.3.1 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
29% identity, 74% coverage: 81:385/413 of query aligns to 169:477/526 of Q9S7B5
- N172 (≠ R84) binding S-adenosyl-L-methionine
- L173 (= L85) binding S-adenosyl-L-methionine
- K181 (≠ E93) binding in monomer B; binding in monomer A
- N187 (≠ Q98) binding in monomer B
- L205 (≠ R116) mutation to R: In mto2-1; causes a strong decrease in the concentration of soluble threonine and over-accumulation of methionine.
2c2bA Crystallographic structure of arabidopsis thaliana threonine synthase complexed with pyridoxal phosphate and s-adenosylmethionine (see paper)
29% identity, 74% coverage: 81:385/413 of query aligns to 94:402/444 of 2c2bA
- binding pyridoxal-5'-phosphate: F127 (= F113), K128 (= K114), D159 (≠ N140), G259 (≠ V241), G260 (≠ A242), N261 (≠ S243), L262 (≠ G244), G263 (≠ S245), N264 (≠ Q246), A321 (≠ S303), H369 (≠ A352), T397 (≠ S380)
- binding s-adenosylmethionine: N97 (≠ R84), L98 (= L85), W100 (≠ R87), W115 (= W101), W115 (= W101), Q246 (= Q228), F247 (≠ L229)
Sites not aligning to the query:
2c2gA Crystal structure of threonine synthase from arabidopsis thaliana in complex with its cofactor pyridoxal phosphate (see paper)
28% identity, 74% coverage: 81:385/413 of query aligns to 112:404/448 of 2c2gA
1pwhA Rat liver l-serine dehydratase- complex with pyridoxyl-(o-methyl- serine)-5-monophosphate (see paper)
28% identity, 65% coverage: 91:358/413 of query aligns to 13:275/327 of 1pwhA
- active site: K41 (= K114), A65 (≠ T138), E194 (≠ Q274), A198 (≠ C278), S200 (≠ P280), A222 (≠ S303), A269 (= A352)
- binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-o-methyl-l-serine: F40 (= F113), K41 (= K114), S64 (= S137), A65 (≠ T138), N67 (= N140), A68 (≠ L141), F136 (≠ N209), G168 (≠ A242), G169 (≠ S243), G170 (= G244), G171 (≠ S245), A222 (≠ S303), G224 (≠ A305)
Sites not aligning to the query:
Q8VBT2 L-serine dehydratase/L-threonine deaminase; SDH; L-serine deaminase; L-threonine dehydratase; TDH; EC 4.3.1.17; EC 4.3.1.19 from Mus musculus (Mouse)
27% identity, 65% coverage: 91:358/413 of query aligns to 13:275/327 of Q8VBT2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
P04968 L-threonine dehydratase biosynthetic IlvA; Threonine deaminase; EC 4.3.1.19 from Escherichia coli (strain K12) (see paper)
24% identity, 64% coverage: 100:365/413 of query aligns to 48:301/514 of P04968
- K62 (= K114) modified: N6-(pyridoxal phosphate)lysine
- N89 (= N140) binding pyridoxal 5'-phosphate
- GGGGL 188:192 (≠ ASGSQ 242:246) binding pyridoxal 5'-phosphate
Sites not aligning to the query:
- 315 binding pyridoxal 5'-phosphate
1tdjA Threonine deaminase (biosynthetic) from e. Coli (see paper)
24% identity, 64% coverage: 100:365/413 of query aligns to 44:297/494 of 1tdjA
- active site: K58 (= K114), A83 (≠ T138), E209 (≠ Q274), S213 (≠ C278), C215 (≠ P280), G237 (≠ S303)
- binding pyridoxal-5'-phosphate: F57 (= F113), K58 (= K114), N85 (= N140), G184 (≠ A242), G185 (≠ S243), G186 (= G244), G187 (≠ S245), G237 (≠ S303), E282 (= E350)
Sites not aligning to the query:
2zr8A Crystal structure of modified serine racemase complexed with serine (see paper)
24% identity, 64% coverage: 99:363/413 of query aligns to 38:290/319 of 2zr8A
- active site: K53 (= K114), S78 (≠ T138), E204 (≠ Q274), G208 (≠ C278), D210 (≠ P280), G232 (vs. gap)
- binding magnesium ion: E204 (≠ Q274), G208 (≠ C278), D210 (≠ P280)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F113), K53 (= K114), S77 (= S137), S78 (≠ T138), N80 (= N140), H81 (≠ L141), P147 (≠ V208), G179 (≠ A242), G180 (≠ S243), G181 (= G244), G182 (≠ S245), G232 (vs. gap), E277 (= E350), T279 (≠ A352)
- binding serine: S78 (≠ T138), R129 (≠ V189), D231 (vs. gap), G232 (vs. gap), A233 (= A301), Q234 (≠ K302), T235 (≠ S303)
Sites not aligning to the query:
2zpuA Crystal structure of modified serine racemase from s.Pombe. (see paper)
24% identity, 64% coverage: 99:363/413 of query aligns to 38:290/319 of 2zpuA
- active site: K53 (= K114), S78 (≠ T138), E204 (≠ Q274), G208 (≠ C278), D210 (≠ P280), G232 (vs. gap)
- binding magnesium ion: E204 (≠ Q274), G208 (≠ C278), D210 (≠ P280)
- binding n-(5'-phosphopyridoxyl)-d-alanine: F52 (= F113), K53 (= K114), S77 (= S137), S78 (≠ T138), N80 (= N140), H81 (≠ L141), P147 (≠ V208), G179 (≠ A242), G180 (≠ S243), G181 (= G244), G182 (≠ S245), G232 (vs. gap), E277 (= E350), T279 (≠ A352)
Sites not aligning to the query:
Query Sequence
>WP_012851176.1 NCBI__GCF_000024385.1:WP_012851176.1
MALDAADLGPATSLTCRECGQSYELGPRYACEQCFGPLEIAYDFAGVTRESIESGPRNIW
RYRGLLPVPANVESTPNTEPGFTRLVRADHLAESLGMQKLWVKDDSGNPTHSFKDRVVAV
ALAAARELGFKVLACPSTGNLANAVAAAAARAGIRSAVFVPADLEQQKIITTAVYGGTFV
TVRGNYDDVNRLASEIAGEQEDWAFVNVNVRPYYAEGSKTLGYEIAEQLGWRLPDQIVIP
VASGSQLTKIDKAFQEFIKLGLVEDKPYKVFGAQATGCSPVAAAFKAGHDVVRPVKPDTI
AKSLAIGNPADGPYVLDVVRRTGGAVEDVSDPEVVEGIRLLAATEGIFAETAGGVTVACL
RKLIASGALDPEAETVIINSGDGLKTLDAVAPVARPSAEIAPTLEDFRASGLA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory