SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012852549.1 NCBI__GCF_000024385.1:WP_012852549.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
55% identity, 99% coverage: 3:395/395 of query aligns to 5:396/654 of P36204

query
sites
P36204

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R36 (= R34) binding substrate
R118 (= R116) binding substrate
R151 (= R153) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
55% identity, 99% coverage: 3:393/395 of query aligns to 4:393/398 of 1vpeA

query
sites
1vpeA

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M

active site: R35 (= R34), K196 (= K199), G353 (= G352), G376 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G197), A195 (≠ S198), K196 (= K199), K200 (= K203), G218 (= G221), A219 (≠ G222), N316 (= N318), P318 (= P320), G320 (= G322), V321 (= V323), E323 (= E325), G352 (= G351), G353 (= G352), D354 (= D353), S355 (= S354)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
53% identity, 100% coverage: 2:395/395 of query aligns to 4:393/394 of 1phpA

query
sites
1phpA

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active site: R36 (= R34), K197 (= K199), G351 (= G352), G374 (= G376)
binding adenosine-5'-diphosphate: G195 (= G197), K201 (= K203), G219 (= G221), G220 (= G222), L237 (= L239), N316 (= N318), P318 (= P320), G320 (= G322), V321 (= V323), E323 (= E325), G350 (= G351), D352 (= D353), S353 (= S354)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
53% identity, 100% coverage: 2:395/395 of query aligns to 4:393/394 of P18912

query
sites
P18912

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K

R

R

L

L

G

G

E

E

L

L

G

E

T

R

E

P

V

V

A

A

F

K

A

T

S

N

D

E

V

A

V

V

G

G

E

D

S

E

A

V

A

K

R

A

T

A

V

V

A

D

G

R

L

L

A

N

D

E

G

G

Q

D

V

V

A

L

L

L

E

E

N

N

L

V

R

R

F

F

E

Y

P

P

G

G

E

E

T

E

S

K

K

N

D

D

D

P

A

E

E

L

R

A

G

K

A

A

F

F

A

A

D

E

K

-

L

-

A

-

L

L

A

A

D

D

L

L

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

A

V

A

H

H

R

R

K

A

H

H

A

A

S

S

V

T

Y

E

D

G

V

I

P

A

R

H

R

Y

L

L

P

P

H

A

A

V

A

A

G

G

G

F

L

L

I

M

V

E

A

K

E

E

V

L

D

E

V

V

L

L

R

G

K

K

L

A

T

L

E

S

E

N

V

P

E

D

R

R

P

P

Y

F

A

T

V

A

V

I

L

I

G

G

S

A

K

K

V

V

S

K

D

D

K
|
K

L

I

G

G

V

V

I

I

D

D

N

N

L

L

N

E

K

K

A

V

D

D

R

N

I

L

L

I

I

I

G

G

M

L

V

A

F

Y

T

T

F

F

L

V

A

K

A

A

Q

L

G

G

H

H

Q

D

V

V

G

G

K

K

S

S

L

L

E

E

Q

E

D

D

Q

K

L

I

D

E

T

L

V

A

R

K

E

S

Y

F

L

M

A

E

R

K

A

A

E

K

K

E

N

K

G

G

V

V

E

R

L

F

V

Y

L

M

P

P

V

V

D

D

I

V

V

V

A

V

A

A

T

D

A

R

F

F

A

A

A

N

D

D

A

A

D

N

H

T

Q

K

V

V

P

P

A

I

D

D

A

A

I

I

P

P

A

A

D

D

R

W

M

S

G

A

L

L

D

D

I

I

G

G

P

P

A

K

S

T

G

R

R

E

L

L

F

Y

A

R

E

D

R

V

L

I

A

R

G

E

A

S

K

K

T

L

V

V

F

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

E

D

P

A

Y

F

S

A

H

H

G

G

T

T

R

K

A

A

V

I

A

A

Q

E

G

A

L

L

I

A

D

E

S

A

G

L

-

D

A

T

F

Y

T

S

V

V

V

I

G

G

G
|
G

D
|
D

S
|
S

A

A

V

V

R

E

S

K

L

F

G

G

F

L

D

A

E

D

S

K

A

-

F

M

S

D

H

H

I

I

S

S

T

T

G

G

A

A

S

S

L

L

E

E

Y

F

L

M

E

E

G

G

K

K

T

Q

L

L

P

P

G

G

L

V

A

V

A

A

L

L

E

E

D

D

K201 (= K203) binding ATP
N316 (= N318) binding ATP
E323 (= E325) binding ATP
GGDS 350:353 (= GGDS 351:354) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
48% identity, 100% coverage: 2:395/395 of query aligns to 4:393/394 of P40924

query
sites
P40924

R

K

T

T

I

L

D

K

D

D

L

I

D

D

V

V

R

K

G

G

K

K

R

V

V

V

L

F

V

C

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

D

D

G

R

E

I

V

T

T

D

D

G

T

R

R

I

I

R

R

A

A

S

A

L

L

P

P

T

T

I

I

N

K

T

H

L

L

R

A

E

D

R

Q

G

G

A

A

K

K

V

V

I

L

V

L

C

A

A

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

Q

E

V

V

K

V

P

E

E

E

F

L

S

R

L

L

A

T

P

P

V

V

A

A

R

A

R

R

L

L

G

G

E

E

L

L

G

G

T

K

E

E

V

V

A

K

F

K

A

A

S

D

D

E

V

A

V

Y

G

G

E

D

S

A

A

V

A

K

R

A

T

Q

V

I

A

S

G

E

L

M

A

K

D

D

G

G

Q

D

V

V

A

L

L

V

L

L

E

E

N

N

L

V

R

R

F

F

E

Y

P

P

G

G

E

E

T

E

S

K

K

N

D

D

D

P

A

E

E

L

R

A

G

K

A

A

F

F

A

A

D

E

K

-

L

-

A

-

L

L

A

A

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

A

V

A

H

H

R

R

K

A

H

H

A

A

S

S

V

T

Y

A

D

G

V

I

P

A

R

E

R

H

L

L

P

P

H

A

A

V

A

A

G

G

G

F

L

L

I

M

V

E

A

K

E

E

V

L

D

D

V

V

L

L

R

G

K

K

L

A

T

V

E
x
S

E

N

V

P

E

D

R

R

P

P

Y

F

A

T

V

A

V

I

L

I

G

G

S

A

K

K

V

V

S

K

D

D

K

K

L

I

G

G

V

V

I

I

D

E

N

S

L

L

N

D

K

K

A

V

D

D

R

N

I

L

L

I

I

I

G

G

M

L

V

A

F

Y

T

T

F

F

L

V

A

K

A

A

Q

L

G

G

H

Y

Q

E

V

V

G

G

K

K

S

S

L

L

E

E

Q

E

D

D

Q

K

L

I

D

E

T

L

V

A

R

K

E

S

Y

F

L

M

A

D

R

R

A

A

E

K

K

E

N

K

G

G

V

V

E

N

L

F

V

Y

L

M

P

P

V

E

D

D

I

V

V

L

A

V

A

A

T

D

A

D

F

F

A

S

A

N

D

D

A

A

D

N

H

V

Q

K

V

I

V

V

P

P

A

I

D

S

A

E

I

I

P

P

A

S

D

D

R

L

M

E

G

A

L

I

D

D

I

I

G

G

P

T

A

K

S
x
T

G

R

R

E

L

T

F

Y

A

A

E

D

R

V

L

I

A

K

G

N

A

S

K

K

T

L

V

V

F

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

M

I

E

D

P

L

Y

F

S

A

H

Q

G

G

T

T

R

K

A

A

V

V

A

A

Q

E

G

A

L

L

I

A

D

E

S

A

G

K

-

D

A

T

F

Y

T

S

V

V

V

I

G

G

D

D

S

S

A

A

V

V

R

E

S

K

L

F

G

G

F

L

D

A

E

D

S

K

A

-

F

M

S

S

H

H

I

I

S

S

T

T

G

G

A

A

S

S

L

L

E

E

Y

F

L

M

E

E

G

G

K

K

T

E

L

L

P

P

G

G

L

V

A

A

L

L

E

N

D

D

S183 (≠ E185) modified: Phosphoserine
T299 (≠ S301) modified: Phosphothreonine

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
45% identity, 99% coverage: 3:395/395 of query aligns to 6:406/407 of 4axxA

query
sites
4axxA

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

V

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

Q

V

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

N

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R

R

F

F

-

H

-

V

-

E

E

E

P

E

G

G

E

K

T

A

S

K

K

A

D

E

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

Q

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

D

D

K
|
K

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

A

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

A

E

E

R

A

L

V

A

T

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

R

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R37 (= R34), K206 (= K199), G364 (= G352), G387 (= G376)
binding adenosine-5'-diphosphate: G204 (= G197), A205 (≠ S198), K210 (= K203), G228 (= G221), G229 (= G222), N327 (= N318), P329 (= P320), G331 (= G322), V332 (= V323), E334 (= E325), G363 (= G351), G364 (= G352), D365 (= D353), T366 (≠ S354)
binding beryllium trifluoride ion: K206 (= K199), K210 (= K203), G363 (= G351)

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
45% identity, 99% coverage: 3:395/395 of query aligns to 6:404/405 of 2wzcA

query
sites
2wzcA

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

V

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

Q

V

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

N

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

P

V

G

E

E

E

T

E

S

G

K

K

-

G

D

E

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

Q

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

D

D

K
|
K

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

A

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

A

E

E

R

A

L

V

A

T

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

R

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R37 (= R34), K204 (= K199), G362 (= G352), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G197), A203 (≠ S198), K204 (= K199), K208 (= K203), G226 (= G221), G227 (= G222), N325 (= N318), P327 (= P320), G329 (= G322), V330 (= V323), E332 (= E325), G361 (= G351), D363 (= D353), T364 (≠ S354)
binding tetrafluoroaluminate ion: R37 (= R34), K204 (= K199), K208 (= K203), G361 (= G351), G362 (= G352), G384 (= G375)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
45% identity, 99% coverage: 3:395/395 of query aligns to 6:404/405 of 2wzbA

query
sites
2wzbA

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

V

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

Q

V

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

N

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

P

V

G

E

E

E

T

E

S

G

K

K

-

G

D

E

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

Q

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

D

D

K
|
K

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

A

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

A

E

E

R

A

L

V

A

T

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

R

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R37 (= R34), K204 (= K199), G362 (= G352), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G197), A203 (≠ S198), K204 (= K199), K208 (= K203), G226 (= G221), G227 (= G222), N325 (= N318), P327 (= P320), G329 (= G322), V330 (= V323), E332 (= E325), G361 (= G351), D363 (= D353), T364 (≠ S354)
binding trifluoromagnesate: K204 (= K199), K208 (= K203), G361 (= G351), G384 (= G375), G385 (= G376)

2x15A The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp and 1,3- bisphosphoglycerate
44% identity, 99% coverage: 3:395/395 of query aligns to 6:407/408 of 2x15A

query
sites
2x15A

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D
|
D

L

F

N
|
N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

V

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H
|
H

L

L

G

G

R
|
R

P

P

K

D

G

G

Q

V

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

N

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R
|
R

F

F

E

H

P

V

G

E

E

E

T

E

S

G

K

K

-

G

-

K

-

A

D

E

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R
|
R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

Q

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

D

D

K
|
K

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

A

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

A

E

E

R

A

L

V

A

T

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

R

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R37 (= R34), K207 (= K199), G365 (= G352), G388 (= G376)
binding adenosine-5'-diphosphate: G205 (= G197), A206 (≠ S198), K207 (= K199), K211 (= K203), G229 (= G221), G230 (= G222), N328 (= N318), P330 (= P320), G332 (= G322), V333 (= V323), E335 (= E325), G364 (= G351), G365 (= G352), D366 (= D353), T367 (≠ S354)
binding adenosine-5'-triphosphate: G205 (= G197), A206 (≠ S198), K207 (= K199), K211 (= K203), G229 (= G221), G230 (= G222), N328 (= N318), G332 (= G322), V333 (= V323), E335 (= E325), G364 (= G351), G365 (= G352), D366 (= D353), T367 (≠ S354), G387 (= G375), G388 (= G376)
binding 1,3-bisphosphoglyceric acid: D22 (= D19), N24 (= N21), R37 (= R34), H61 (= H57), R64 (= R60), R121 (= R116), R162 (= R153), K207 (= K199), K211 (= K203), G364 (= G351), G387 (= G375), G388 (= G376)

1vjcA Structure of pig muscle pgk complexed with mgatp (see paper)
44% identity, 99% coverage: 3:395/395 of query aligns to 7:415/416 of 1vjcA

query
sites
1vjcA

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

I

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

Q

I

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

D

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

P

V

G

E

E

E

T

E

S

G

K

K

-

G

-

K

-

D

-

A

-

S

-

G

-

S

-

K

-

V

-

K

-

A

D

D

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

K

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S

A

K
|
K

V

V

S

A

D

D

K
|
K

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

S

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

S

E

E

R

A

L

V

A

A

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

Q

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S

T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R38 (= R34), K215 (= K199), G373 (= G352), G396 (= G376)
binding adenosine-5'-triphosphate: G213 (= G197), K219 (= K203), G238 (= G222), G340 (= G322), V341 (= V323), E343 (= E325), D374 (= D353)

2wzdA The catalytically active fully closed conformation of human phosphoglycerate kinase k219a mutant in complex with adp, 3pg and aluminium trifluoride (see paper)
45% identity, 99% coverage: 3:395/395 of query aligns to 6:404/405 of 2wzdA

query
sites
2wzdA

T

T

I

L

D

D

D

K

L

L

D

D

V

V

R

K

G

G

K

K

R

R

V

V

L

V

V

M

R

R

S

V

D

D

L

F

N

N

V

V

P

P

L

M

D

K

G

N

D

N

R

Q

I

I

T

T

D

N

G

Q

R
|
R

I

I

R

K

A

A

S

A

L

V

P

P

T

S

I

I

N

K

T

F

L

C

R

L

E

D

R

N

G

G

A

A

K

K

-

S

V

V

I

V

V

L

C

M

A

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

Q

V

V

P

K

M

P

P

E

D

-

K

F

Y

S

S

L

L

A

E

P

P

V

V

A

A

R

V

R

E

L

L

G

K

E

S

L

L

G

G

T

K

E

D

V

V

A

L

F

F

A

L

S

K

D

D

V

C

V

V

G

G

E

P

S

E

A

V

A

E

R

K

T

A

V

C

A

A

G

N

L

P

A

A

D

A

G

G

Q

S

V

V

A

I

L

L

E

E

N

N

L

L

R

R

F

F

E

H

P

V

G

E

E

E

T

E

S

G

K

K

-

G

D

E

D

P

A

A

E

K

R

I

G

E

A

A

F

F

A

R

D

A

K

S

L

L

A

S

A

K

L

L

A

G

D

D

L

V

Y

Y

V

V

G

N

D

D

G

A

F

F

G

G

A

T

V

A

H

H

R

R

K

A

H

H

A

S

S

S

V

M

Y

V

D

G

V

V

P

-

R

-

R

N

L

L

P

P

H

Q

A

K

A

A

G

G

L

F

I

L

V

M

A

K

-

K

E

E

V

L

D

N

V

Y

L

F

R

A

K

K

L

A

T

L

E

E

E

S

V

P

E

E

R

R

P

P

Y

F

A

L

V

A

V

I

L

L

G

G

G
|
G

S
x
A

K
|
K

V

V

S

A

D

D

K

A

L

I

G

Q

V

L

I

I

D

N

N

N

L

M

L

L

N

D

K

K

A

V

D

N

R

E

I

M

L

I

I

I

G

G

G
|
G

M

M

V

A

F

F

T

T

F

F

L

L

A

K

A

V

-

L

Q

N

G

N

H

M

Q

E

V

I

G

G

K

T

S

S

L

L

L

F

E

D

Q

E

D

E

Q

G

L

A

D

K

T

I

V

V

R

K

E

D

Y

L

L

M

A

S

R

K

A

A

E

E

K

K

N

N

G

G

V

V

E

K

L

I

V

T

L

L

P

P

V

V

D

D

I

F

V

V

A

T

A

A

T

D

A

K

F

F

A

D

A

E

D

N

A

A

D

K

H

T

-

G

Q

Q

V

A

V

T

P

V

A

A

D

S

A

G

I

I

P

P

A

A

D

G

R

W

M

M

G

G

L

L

D

D

I

C

G

G

P

P

A

E

S

S

G

S

R

K

L

K

F

Y

A

A

E

E

R

A

L

V

A

T

G

R

A

A

K

K

T

Q

V

I

F

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

E

E

P

A

Y

F

S

A

H

R

G

G

T

T

R

K

A

A

V

L

A

M

Q

D

G

E

L

V

I

V

D

K

S

A

-

T

-

S

-

R

G

G

A

C

F

I

T

T

V

I

G

G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

C

V

C

R

A

S

K

L

W

G

N

F

-

D

T

E

E

S

D

A

K

F

V

S

S

H

H

I

V

S

S

T

T

G

G

G
|
G

A

A

S

S

L

L

E

E

Y

L

L

L

E

E

G

G

K

K

T

V

L

L

P

P

G

G

L

V

A

D

A

A

L

L

E

S

D

N

active site: R37 (= R34), K204 (= K199), G362 (= G352), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G197), A203 (≠ S198), K204 (= K199), G226 (= G221), G227 (= G222), N325 (= N318), P327 (= P320), G329 (= G322), V330 (= V323), E332 (= E325), G361 (= G351), D363 (= D353), T364 (≠ S354)
binding aluminum fluoride: R37 (= R34), K204 (= K199), G361 (= G351), G362 (= G352), G384 (= G375)

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
45% identity, 99% coverage: 3:395/395 of query aligns to 8:414/416 of P00560

query
sites
P00560

T

S

I

V

D

Q

D

D

L

L