SitesBLAST – Find functional sites

 

SitesBLAST

Comparing WP_012853158.1 NCBI__GCF_000024385.1:WP_012853158.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

1dbfA Chorismate mutase from bacillus subtilis at 1.30 angstrom (see paper)
47% identity, 99% coverage: 1:119/120 of query aligns to 1:120/127 of 1dbfA

query
sites
1dbfA
M
 
M
A
 
M
V
 
I
R
 
R
A
 
G
I
 
I
R
|
R
G
 
G
A
 
A
T
 
T
Q
 
T
V
 
V
E
|
E
A
 
R
D
 
D
D
 
T
R
 
E
E
 
E
Q
 
E
I
 
I
L
 
L
E
 
Q
A
 
K
T
 
T
T
x
K
E
 
Q
L
 
L
V
 
L
T
 
E
E
 
K
V
 
I
M
 
I
R
 
E
R
 
E
N
 
N
G
 
H
L
 
T
S
 
K
T
 
P
D
 
E
D
 
D
V
 
V
I
 
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Q
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M
I
 
L
F
 
L
T
 
S
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A
T
 
T
P
 
P
D
 
D
L
 
L
T
 
H
A
 
A
E
 
V
F
 
F
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P
A
 
A
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K
A
 
A
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R
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x
E
L
 
L
-
 
S
G
 
G
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W
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Q
E
 
Y
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P
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V
L
x
T
C
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C
A
 
M
T
 
Q
E
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E
L
 
M
D
 
D
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P
 
T
H
 
G
A
 
G
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L
P
 
K
R
 
K
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C
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I
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V
M
 
M
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M
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V
E
 
Q
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T
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D
R
 
V
P
 
P
R
 
Q
S
 
D
Q
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Q
I
 
I
Q
 
R
H
 
H
V
 
V
Y
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Y
L
 
L
R
 
E
G
x
K
A
 
A
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V
A
 
V
L
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L
R
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R
L
 
P
D
 
D
I
 
L
A
 
S

Sites not aligning to the query:

P19080 Chorismate mutase AroH; CM; EC 5.4.99.5 from Bacillus subtilis (strain 168) (see 2 papers)
46% identity, 99% coverage: 1:119/120 of query aligns to 1:120/127 of P19080

query
sites
P19080
M
 
M
A
 
M
V
 
I
R
 
R
A
 
G
I
 
I
R
|
R
G
 
G
A
 
A
T
 
T
Q
 
T
V
 
V
E
 
E
A
 
R
D
 
D
D
 
T
R
 
E
E
 
E
Q
 
E
I
 
I
L
 
L
E
 
Q
A
 
K
T
 
T
T
 
K
E
 
Q
L
 
L
V
 
L
T
 
E
E
 
K
V
 
I
M
 
I
R
 
E
R
 
E
N
 
N
G
 
H
L
 
T
S
 
K
T
 
P
D
 
E
D
 
D
V
 
V
I
 
V
S
 
Q
V
 
M
I
 
L
F
 
L
T
 
S
A
 
A
T
 
T
P
 
P
D
 
D
L
 
L
T
 
H
A
 
A
E
 
V
F
 
F
P
 
P
A
 
A
L
 
K
A
 
A
A
 
V
R
 
R
K
 
E
L
 
L
-
 
S
G
 
G
F
 
W
H
 
Q
E
 
Y
V
 
V
P
 
P
L
 
V
L
x
T
C
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C
A
x
M
T
x
Q
E
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E
L
 
M
D
 
D
V
 
V
P
 
T
H
 
G
A
 
G
L
 
L
P
 
K
R
 
K
V
 
C
I
 
I
R
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R
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V
M
 
M
A
 
M
H
 
T
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V
E
 
Q
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T
D
 
D
R
 
V
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P
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Q
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D
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Q
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I
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R
H
 
H
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V
Y
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Y
L
 
L
R
 
E
G
 
K
A
 
V
V
 
V
A
 
V
L
 
L
R
 
R
L
 
P
D
 
D
I
 
L
A
 
S

3zp7A Arg90cit chorismate mutase of bacillus subtilis in complex with chorismate and prephenate (see paper)
47% identity, 96% coverage: 1:115/120 of query aligns to 1:116/117 of 3zp7A

query
sites
3zp7A
M
 
M
A
 
M
V
 
I
R
 
R
A
 
G
I
 
I
R
|
R
G
 
G
A
 
A
T
 
T
Q
 
T
V
 
V
E
 
E
A
 
R
D
 
D
D
 
T
R
 
E
E
 
E
Q
 
E
I
 
I
L
 
L
E
 
Q
A
 
K
T
 
T
T
 
K
E
 
Q
L
 
L
V
 
L
T
 
E
E
 
K
V
 
I
M
 
I
R
 
E
R
 
E
N
 
N
G
 
H
L
 
T
S
 
K
T
 
P
D
 
E
D
 
D
V
 
V
I
 
V
S
 
Q
V
 
M
I
 
L
F
 
L
T
 
S
A
 
A
T
 
T
P
 
P
D
 
D
L
 
L
T
 
H
A
 
A
E
 
V
F
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F
P
 
P
A
|
A
L
x
K
A
 
A
A
 
V
R
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R
K
 
E
L
 
L
-
 
S
G
 
G
F
 
W
H
 
Q
E
 
Y
V
 
V
P
 
P
L
x
V
L
x
T
C
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C
A
 
M
T
 
Q
E
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E
L
 
M
D
 
D
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V
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T
H
 
G
A
 
G
L
 
L
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K
R
 
K
V
 
C
I
 
I
R
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R
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V
M
 
M
A
 
M
H
 
T
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V
E
 
Q
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T
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D
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V
P
 
P
R
 
Q
S
 
E
Q
 
Q
I
 
I
Q
 
R
H
 
H
V
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V
Y
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Y
L
 
L
R
 
E
G
 
K
A
 
A
V
 
V
A
 
V
L
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L
R
 
R

2chtA Crystal structures of the monofunctional chorismate mutase from bacillus subtilis and its complex with a transition state analog (see paper)
47% identity, 93% coverage: 3:114/120 of query aligns to 2:114/114 of 2chtA

query
sites
2chtA
V
 
I
R
 
R
A
 
G
I
 
I
R
|
R
G
 
G
A
 
A
T
 
T
Q
 
T
V
 
V
E
 
E
A
 
R
D
 
D
D
 
T
R
 
E
E
 
E
Q
 
E
I
 
I
L
 
L
E
 
Q
A
 
K
T
 
T
T
 
K
E
 
Q
L
 
L
V
 
L
T
 
E
E
 
K
V
 
I
M
 
I
R
 
E
R
 
E
N
 
N
G
 
H
L
 
T
S
 
K
T
 
P
D
 
E
D
 
D
V
 
V
I
 
V
S
 
Q
V
 
M
I
 
L
F
 
L
T
 
S
A
 
A
T
 
T
P
 
P
D
 
D
L
 
L
T
 
H
A
 
A
E
 
V
F
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F
P
 
P
A
|
A
L
x
K
A
 
A
A
 
V
R
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R
K
 
E
L
 
L
-
 
S
G
 
G
F
 
W
H
 
Q
E
 
Y
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V
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P
L
x
V
L
x
T
C
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C
A
 
M
T
 
Q
E
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E
L
 
M
D
 
D
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V
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T
H
 
G
A
 
G
L
 
L
P
 
K
R
 
K
V
 
C
I
 
I
R
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R
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V
M
 
M
A
 
M
H
 
T
V
 
V
E
 
Q
T
 
T
D
 
D
R
 
V
P
 
P
R
 
Q
S
 
D
Q
 
Q
I
 
I
Q
 
R
H
 
H
V
 
V
Y
|
Y
L
 
L
R
 
E
G
 
K
A
 
A
V
 
V
A
 
V
L
|
L

1comA The monofunctional chorismate mutase from bacillus subtilis: structure determination of chorismate mutase and its complexes with a transition state analog and prephenate, and implications on the mechanism of enzymatic reaction (see paper)
47% identity, 93% coverage: 3:114/120 of query aligns to 2:114/114 of 1comA

query
sites
1comA
V
 
I
R
 
R
A
 
G
I
 
I
R
|
R
G
 
G
A
 
A
T
 
T
Q
 
T
V
 
V
E
 
E
A
 
R
D
 
D
D
 
T
R
 
E
E
 
E
Q
 
E
I
 
I
L
 
L
E
 
Q
A
 
K
T
 
T
T
 
K
E
 
Q
L
 
L
V
 
L
T
 
E
E
 
K
V
 
I
M
 
I
R
 
E
R
 
E
N
 
N
G
 
H
L
 
T
S
 
K
T
 
P
D
 
E
D
 
D
V
 
V
I
 
V
S
 
Q
V
 
M
I
 
L
F
 
L
T
 
S
A
 
A
T
 
T
P
 
P
D
 
D
L
 
L
T
 
H
A
 
A
E
 
V
F
|
F
P
 
P
A
|
A
L
 
K
A
 
A
A
 
V
R
|
R
K
 
E
L
 
L
-
 
S
G
 
G
F
 
W
H
 
Q
E
 
Y
V
 
V
P
 
P
L
x
V
L
x
T
C
|
C
A
 
M
T
 
Q
E
|
E
L
 
M
D
 
D
V
 
V
P
 
T
H
 
G
A
 
G
L
 
L
P
 
K
R
 
K
V
 
C
I
 
I
R
|
R
L
 
V
M
 
M
A
 
M
H
 
T
V
 
V
E
 
Q
T
 
T
D
 
D
R
 
V
P
 
P
R
 
Q
S
 
D
Q
 
Q
I
 
I
Q
 
R
H
 
H
V
 
V
Y
|
Y
L
 
L
R
 
E
G
 
K
A
 
A
V
 
V
A
 
V
L
 
L

Query Sequence

>WP_012853158.1 NCBI__GCF_000024385.1:WP_012853158.1
MAVRAIRGATQVEADDREQILEATTELVTEVMRRNGLSTDDVISVIFTATPDLTAEFPAL
AARKLGFHEVPLLCATELDVPHALPRVIRLMAHVETDRPRSQIQHVYLRGAVALRLDIAQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory