SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012964546.1 NCBI__GCF_000025505.1:WP_012964546.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 1 hits to proteins with known functional sites (download)

Q58487 Mevalonate kinase; MK; MVK; EC 2.7.1.36 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
25% identity, 75% coverage: 23:227/273 of query aligns to 54:277/312 of Q58487

query
sites
Q58487

A

S

F

L

A

G

L

L

N

N

L

L

K

N

T

E

R

I

V

K

R

N

I

I

I

N

P

P

G

N

K

N

E

F

G

G

D

D

F

F

E

-

S

-

R

K

A

Y

L

C

E

L

C

C

V

A

V

I

K

K

K

N

V

T

F

L

R

D

S

Y

L

L

N

N

I

I

D

E

G

P

K

K

-

T

-

G

-

F

-

K

V

I

K

N

V

I

E

S

S

S

E

K

I

I

P

P

R

I

R

S

S

C

G

G

L

L

G

G

S

S

S

S

S

A

A

S

V

I

V

T

N

I

A

G

L

T

M

I

C

K

A

A

A

V

Y

S

K

G

Y

F

L

Y

N

N

K

K

E

E

L

L

E

K

A

D

H

D

S

E

I

I

L

A

R

K

A

L

N

G

A

Y

R

M

I

V

S

E

L

K

E

E

C

-

G

-

M

I

S

Q

Y

G

T

K

G

A

A

S

F

I

D

T

D

D

A

T

S

S

A

T

S

I

L

T

L

Y

G

K

G

G

V

I

V

L

F

E

S

I

D

K

N

N

A

N

K

K

M

F

K

R

L

K

F

I

K

K

-

G

-

E

-

F

R

E

E

E

S

F

L

L

E

K

G

N

K

C

A

K

A

F

I

L

L

I

I

V

P

Y

A

A

W

E

E

K

R
|
R

K

K

N

K

V

K

-

T

-

A

-

E

-

L

-

V

-

N

D

E

L

V

K

A

K

K

I

I

R

E

E

N

R

K

R

D

E

E

-

I

-

F

-

K

E

E

V

I

E

D

R

K

A

V

F

I

K

D

L

E

A

A

M

L

E

K

G

I

K

K

L

N

R

K

E

E

-

D

-

F

-

G

A

K

M

L

Y

M

V

T

N

K

S

N

Y

H

E

E

Y

L

C

L

R

K

A

K

L

L

N

N

L

I

P

S

-

T

-

P

-

K

F

L

E

D

P

R

V

I

V

V

A

D

A

I

Y

G

N

N

-

R

L

F

G

G

L

F

N

G

A

A

G
x
K

L

L

S

T

G

G

N

A

G

G

R196 (= R162) mutation to K: 13.5-fold decrease in affinity for mevalonate, whereas only little effect on affinity for ATP and on reaction rate.; mutation to Q: 1900-fold decrease in catalytic efficiency.; mutation to V: 63-fold decrease in catalytic efficiency.
K272 (≠ G222) mutation K->R,A: 13- to 26-fold decrease in catalytic efficiency. Still thermostable.

Query Sequence

>WP_012964546.1 NCBI__GCF_000025505.1:WP_012964546.1
MVEGKAFAAGTIVNALAALKGVAFALNLKTRVRIIPGKEGDFESRALECVVKKVFRSLNI
DGKVKVESEIPRRSGLGSSSAVVNALMCAAYKYLNKELEAHSILRANARISLECGMSYTG
AFDDASASLLGGVVFSDNAKMKLFKRESLEGKAAILIPAWERKNVDLKKIRERREEVERA
FKLAMEGKLREAMYVNSYEYCRALNLPFEPVVAAYNLGLNAGLSGNGPSYVAFGENVEDV
KSVWESFGKVIFVDIVNVPAEDVKISGSLFIQT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory