SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012964674.1 NCBI__GCF_000025505.1:WP_012964674.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5odhG Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus soaked with heterodisulfide for 3.5 minutes (see paper)
52% identity, 82% coverage: 2:637/777 of query aligns to 4:648/653 of 5odhG

query
sites
5odhG

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binding flavin-adenine dinucleotide: I148 (= I146), G149 (= G147), G150 (= G148), G151 (= G149), V152 (= V150), A153 (= A151), E172 (= E170), K173 (= K171), G179 (= G177), R180 (≠ K178), L184 (= L182), T187 (≠ V185), F188 (= F186), I327 (≠ T326), G328 (= G327), Y329 (= Y328), Y405 (≠ V403), K408 (= K406), S499 (≠ G492), G538 (= G531), K544 (= K537), D545 (= D538), I546 (= I539), S549 (= S542)
binding fe (iii) ion: C45 (= C43), H75 (= H73)
binding iron/sulfur cluster: C10 (= C8), C12 (= C10), C45 (= C43), A69 (= A67), C70 (= C68), H75 (= H73), C245 (= C243), T246 (≠ K244), G247 (= G245), C248 (= C246), C251 (= C250), C255 (= C254), P256 (= P255), C292 (= C291), I293 (= I292), E294 (≠ G293), C295 (= C294), C298 (= C297), C302 (= C301), C385 (= C384), C398 (= C396), S399 (= S397), N400 (≠ R398), C402 (= C400), C403 (= C401), R430 (= R428), C583 (= C576), G585 (= G578), C586 (= C579), G587 (≠ R580), C589 (= C582), C593 (= C586), A609 (≠ P602), C616 (= C605), K617 (≠ V606), C619 (= C608), G620 (= G609), C622 (= C611), C626 (= C615), S628 (≠ A617)

5odcA Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
52% identity, 82% coverage: 2:637/777 of query aligns to 4:648/653 of 5odcA

query
sites
5odcA

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A

A

M

A

E

I

L

P

I

L

F

A

G

K

G

G

E

E

A

V

E

E

F

V

D

E

P

P

Y

I

N

I

A
|
A

Y

S

V

V

N

D

E

A

E

E

K

I

C
|
C

I

G

G

G

C
|
C

R
x
G

I
x
V

C
|
C

E

V

E

K

V

Q

C
|
C

N

P

F

Y

N

G

A

A

V

P

T

R

F

L

E

V

N

E

K

K

K

D

A

G

K

K

I

V

D

V

P
x
A

N

E

A

V

-

I

-

S

-

A

-

L

C
|
C

V
x
K

M

G

C
|
C

G
|
G

V

T

C
|
C

A

P

A

A

S

G

C
|
C

P

P

A
x
S

D

G

A

A

I
x
L

D

E

L

Q

G

D

F

H

F

F

K

K

E

T

D

I

A

Q

I

L

V

F

A

K

M

Q

I

I

D

E

A

G

L

M

binding flavin-adenine dinucleotide: I148 (= I146), G149 (= G147), G151 (= G149), V152 (= V150), A153 (= A151), E172 (= E170), K173 (= K171), G179 (= G177), R180 (≠ K178), L184 (= L182), T187 (≠ V185), F188 (= F186), A326 (= A325), I327 (≠ T326), G328 (= G327), Y329 (= Y328), A351 (≠ T350), L352 (= L351), E355 (= E354), Y405 (≠ V403), K408 (= K406), S499 (≠ G492), G538 (= G531), K544 (= K537), D545 (= D538), I546 (= I539)
binding iron/sulfur cluster: C10 (= C8), C12 (= C10), C45 (= C43), A69 (= A67), C70 (= C68), H75 (= H73), C245 (= C243), T246 (≠ K244), G247 (= G245), C248 (= C246), G249 (≠ D248), C251 (= C250), C255 (= C254), P256 (= P255), Y285 (≠ A284), C292 (= C291), E294 (≠ G293), C295 (= C294), G296 (≠ R295), C298 (= C297), C302 (= C301), Q384 (= Q383), C385 (= C384), R389 (= R388), C398 (= C396), S399 (= S397), N400 (≠ R398), C402 (= C400), C403 (= C401), R430 (= R428), A576 (= A569), C583 (= C576), C586 (= C579), G587 (≠ R580), V588 (≠ I581), C589 (= C582), C593 (= C586), A609 (≠ P602), C616 (= C605), K617 (≠ V606), C619 (= C608), G620 (= G609), C622 (= C611), C626 (= C615), S628 (≠ A617), L631 (≠ I620)

7bkdA Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (heterodislfide reductase core and mobile arm in conformational state 1, composite structure) (see paper)
48% identity, 82% coverage: 2:637/777 of query aligns to 4:661/664 of 7bkdA

query
sites
7bkdA

R

R

I

I

G

G

V

V

F

F

V

V

C
|
C

H

H

C
|
C

G

G

L

T

N

N

I

I

A

A

R

G

I

S

V

M

D

S

V

I

Q

D

E

D

V

V

N

N

Y

Y

A

A

K

K

N

T

L

L

E

P

D

Y

V

V

Y

A

F

V

S

A

T

D

D

Q

L

Y

K

Q

Y

Y

A

M

C
|
C

S

S

D

T

S

P

G

G

Q

Q

E

K

I

I

I

D

K

D

A

A

I

I

K

K

E

E

N

Y

R

N

L

L

D

T

A

G

V

V

I

V

A

A

A
|
A

C
|
C

S

S

P

P

K

R

L

L

H

H

E

E

H

P

T

T

F

F

R

R

K

T

A

A

A

T

M

K

R

E

A

G

G

G

L

L

N

N

P

P

Y

F

M

R

V

F

L

E

M

M

A

A

N

N

I

I

R

R

E

E

Q

Q

C

N

S

S

W

W

V

V

H

H

Q

M

E

H

-

G

H

M

P

W

K

D

A

E

A

A

T

T

E

Q

K

K

A

A

K

K

D

D

L

Q

V

V

R

R

M

M

A

A

V

V

A

A

A

K

A

A

R

A

K

K

L

L

E

E

P

D

L

L

S

V

R

P

K

K

R

S

I

V

E

P

V

V

K

E

K

K

S

T

A

A

V

M

V

V

I
x
V

G
|
G

G

G

G
|
G

V
|
V

A

A

G

G

I

M

E

Q

A

A

L

L

T

D

L

L

A

A

N

S

A

A

G

G

V

I

K

K

V

T

Y

Y

L

L

I

I

E
|
E

K
x
R

A

T

P

P

T

T

I

I

G

G

G
|
G

K
x
R

M

M

A

S

T

Q

L
|
L

N

D

E

K

V
x
T

F
|
F

P

P

T

T

N

L

D

D

C

C

S

S

I

Q

C

C

I

I

L

L

A

T

P

P

K

K

M

M

S

V

E

D

A

V

F

G

N

R

H

H

E

P

N

N

I

I

E

E

V

M

I

M

T

T

N

Y

A

T

E

E

V

V

L

E

E

K

V

V

S

E

G

G

H

Y

V

I

G

G

N

N

F

F

K

D

V

V

K

T

V

L

R

R

K

K

H

K

P

A

R

R

Y

G

V

V

-

L

-

T

-

P

D

T

E

E

N

A

K

T

C

A

K

K

-

G

-

I

-

V

-

G

-
x
C

G

N

C
x
G

I
x
C

D
x
G

D

D

C
|
C

S

S

S

A

V

V

C
|
C

P
|
P

V

V

E

I

V

K

P

P

N

N

E

P

F

F

D

E

Y

M

T

G

I

M

G

A

V

P

R

R

K

K

A
|
A

I
|
I

Y

Y

L

I

P

Y

I

H

P

A

Q

Q

S

V

T

M

P

P

L

L

Y

I

A
x
Y

A

T

I

V

D

D

W

F

E

D

H

S

C
|
C

I
x
V

G
x
K

C
|
C

R
x
G

L

L

C
|
C

E

V

K

E

A

A

C
|
C

-

G

E

D

P

K

K

K

A
|
A

V

I

D

D

F

L

N

E

Q

M

K

Q

P

D

E

E

D

F

L

I

E

T

I

V

E

K

A

V

G

G

V

T

I

A

I

V

V

L

A
|
A

T
|
T

G
|
G

Y
|
Y

K

E

P

L

F

F

D

P

A

I

R

E

R

N

K

K

E

R

E

E

Y

W

G

G

Y

Y

G

K

V

Q

Y

F

K

D

N

N

V

V

I

I

T

N

T

A

L

L

E

E

L

F

E
|
E

R

R

L

L

L

I

S

C

A

A

S

S

G

G

P

P

T

T

L

G

G

G

N

H

L

L

Y

V

R

R

P

P

S

S

D

D

S

G

S

K

V

T

P

P

R

M

K

K

I

V

A

G

F

F

I

V

Q
x
L

C
|
C

V

A

G

G

S

S

R

R

D

D

V

N

K

T

-

G

-

I

T

G

N

K

K

P

Y

Y

C
|
C

S
|
S

R
|
R

V

F

C
|
C

M

M

V
x
Y

S

S

I

L

K
|
K

N

H

A

A

Y

H

I

Q

I

I

K

M

E

E

R

K

Y

I

P

P

E

G

A

A

D

V

V

A

S

Y

V

L

F

F

F

Y

I

M

D

D

I

I

R
|
R

A

S

F

F

G

G

R

K

M

M

Y

Y

E

E

F

F

F

Y

A

Y

R

R

A

I

Q

Q

E

H

K

E

G

G

I

A

R

K

F

F

I

I

R

R

G

G

R

R

V

V

A

A

E

N

I

V

Y

L

E

E

-

D

L

K

E

E

N

T

G

K

N

N

L

L

I

H

L

V

T

F

Y

T

E

E

N

D

T

T

L

L

T

L

G

G

E

R

I

P

K

V

E

D

E

V

E

E

F

V

E

D

L

L

V

L

V

V

L

L

S

A

I

A

G

A

M

V

-

Q

-

P

-

N

E

E

G

G

N

A

T

N

D

E

L

L

A

R

N

K

K

K

L

F

G

G

I

V

S

S

V

A

G

S

E

Q

D

D

G

G

F

W

Y

M

D

L

V

E

A

A

H

H

P

P

K

K

L

L

R

N

P

P

A

C

E

G

T

T

D

T

V

T

K

A

G

G

I

V

F

F

L

L

A

A

G

G

A
x
V

A

C

S

Q

G

G

P

P

K
|
K

D

D

I
|
I

Q

P

D

D

S

T

V

V

A

A

S

Q

A

A

G

E

L

G

A

A

S

S

K

A

A

A

M

S

E

I

L

P

I

I

F

H

G

M

G

G

E

E

A

V

E

E

F

L

D

E

P

P

Y

Y

N

F

A

A

Y

M

V

C

N

I

E

D

E

E

K

L

C
|
C

I
x
A

G

G

C
|
C

R
x
G

I

M

C
|
C

E

V

E

N

V

L

C
|
C

N

P

F

Y

N

S

A

A

V
x
L

T

S

F

L

E

G

N

E

K

K

K

N

A

G

K

R

-

T

-

V

-

M

-

V

I

V

D

T

P

E

N

A

A

K

C
|
C

V
x
K

M

G

C
|
C

G
|
G

V

T

C
|
C

A

G

S

F

C
|
C

P

P

A

G

D

G

A

A

I
|
I

D

K

L

M

G

Q

F

H

F

F

K

T

E

T

D

P

A

Q

I

I

V

V

A

A

M

Q

I

I

D

D

A

A

L

F

binding flavin-adenine dinucleotide: V149 (≠ I146), G150 (= G147), G152 (= G149), V153 (= V150), E173 (= E170), R174 (≠ K171), G180 (= G177), R181 (≠ K178), L185 (= L182), T188 (≠ V185), F189 (= F186), A339 (= A325), T340 (= T326), G341 (= G327), Y342 (= Y328), E368 (= E354), Y419 (≠ V403), K422 (= K406), V552 (≠ A532), K557 (= K537), I559 (= I539)
binding iron/sulfur cluster: C10 (= C8), C12 (= C10), C45 (= C43), A69 (= A67), C70 (= C68), C257 (vs. gap), G259 (≠ C246), C260 (≠ I247), G261 (≠ D248), C263 (= C250), C267 (= C254), P268 (= P255), A284 (= A271), I285 (= I272), Y297 (≠ A284), C304 (= C291), V305 (≠ I292), K306 (≠ G293), C307 (= C294), G308 (≠ R295), C310 (= C297), C314 (= C301), A319 (= A305), L397 (≠ Q383), C398 (= C384), C412 (= C396), S413 (= S397), R414 (= R398), C416 (= C400), C417 (= C401), R444 (= R428), C596 (= C576), A597 (≠ I577), C599 (= C579), G600 (≠ R580), C602 (= C582), C606 (= C586), L611 (≠ V591), C629 (= C605), K630 (≠ V606), C632 (= C608), G633 (= G609), C635 (= C611), C639 (= C615), I644 (= I620)

6tjrA Structure of hdra-like subunit from hyphomicrobium denitrificans (see paper)
33% identity, 31% coverage: 307:543/777 of query aligns to 88:325/340 of 6tjrA

query
sites
6tjrA

D

N

F

F

N

T

Q

A

K

K

P

L

E

S

D

D

-

G

L

T

E

S

I

I

E

D

A

C

G

A

V

S

I

A

I

I

V

L

A

T

T
|
T

G
|
G

Y
x
F

K

S

P
x
H

F

F

D

D

A

S

R

V

R

N

K

K

E

P

E

E

Y

W

G

G

Y

F

G

G

V

M

Y

F

K

P

N

D

V

V

I

V

T

T

T
|
T

L

T

E

Q

L

V

E

E

R

Q

L

M

L

I

S

S

A

-

S

S

G

G

P

K

T

G

L

V

G

R

N

C

L

L

Y

-

R

-

P

-

S

S

D

D

S

G

S

R

V

K

P

P

R

K

K

R

I

V

A

A

F

I

I

L

Q

L

C
|
C

V

V

G

G

S
|
S

R
|
R

D

D

V

R

K

Q

T

I

N

G

K

R

-

E

Y

W

C
|
C

S
|
S

R
x
K

V
x
I

C
|
C

M

T

V
|
V

S

S

I

A

K

N

N

L

A

A

Y

M

I

E

I

I

K

R

E

E

R

E

Y

L

P

P

E

D

A

C

D

H

V

V

S

Y

V

I

F

Y

I

M

D

D

I

I

R
|
R

A

T

F

F

G

G

R

H

M

Y

Y

E

E

S

E

D

F

Y

A

W

R

R

A

S

Q

Q

E

E

K

E

-

F

G

K

I

V

R

K

F

Y

I

I

R

K

G

A

R

R

V

I

A

A

E

E

I

V

Y

T

E

S

L

-

E

D

N

G

G

K

N

Q

L

L

I

I

L

V

T

K

Y

G

E

E

N

D

T

T

L

L

T

V

G

K

E

R

I

P

K

I

E

T

E

I

E

P

F

F

E

D

L

M

V

V

L

H

S

A

I

I

G

G

M

M

E

D

G

P

N
|
N

T

V

D
|
D

-

N

-

M

-

T

L

I

A

S

N

A

K

I

L

F

G

G

I

V

S

E

V

L

G

H

E

K

D

H

G

G

F

Y

Y

I

D

A

V

R

A

K

H

D

P

T

K

Y

L

G

R

L

P

M

A

G

E

A

T

T

D

S

V

R

K

P

G

G

I

V

F

F

L

V

A

A

G

G

A
x
S

A

A

S

I

G

G

P

P

K
x
E

D
x
T

I
|
I

Q

D

D

D

S

S

V

I

binding flavin-adenine dinucleotide: T108 (= T326), G109 (= G327), F110 (≠ Y328), H112 (≠ P330), T132 (= T350), I178 (≠ V399), V182 (= V403), N272 (= N493), D274 (= D495), S314 (≠ A532), E319 (≠ K537), T320 (≠ D538), I321 (= I539)
binding iron/sulfur cluster: C162 (= C384), S165 (= S387), R166 (= R388), C175 (= C396), S176 (= S397), K177 (≠ R398), C179 (= C400), C180 (= C401), R207 (= R428)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 7, 9, 10, 11, 30, 31, 32, 37, 38, 44, 47, 48

5odcD Heterodisulfide reductase / [nife]-hydrogenase complex from methanothermococcus thermolithotrophicus at 2.3 a resolution (see paper)
47% identity, 16% coverage: 644:769/777 of query aligns to 3:128/138 of 5odcD

query
sites
5odcD

E

E

P

P

L

K

I

I

L

I

I

G

F

F

A

C

C
|
C

H

N

F

W

C

C

S

T

Y

Y

G

G

A

G

L

A

D

D

L

T

A

A

G

G

T

V

T

G

K

R

T

M

Q

Q

Y

Y

S

P

P

P

N

S

V

I

R

R

V

I

I

I

R

R

T

V

L

M

C
|
C

S
|
S

G

G

R

R

V

I

D

E

P

P

E

S

W

L

I

I

L

L

R

K

A

A

L

F

K

K

R

E

G

G

I

A

D

D

G

G

V

V

M

F

V

V

T

G

G

G

C
|
C

R

H

L

L

G

G

E

D

C
|
C

H
|
H

F

Y

R

D

V

S

G

G

N

N

Y

Y

H

K

A

W

V

Q

D

R

R

R

I

V

K

M

A

M

L

L

K

Y

K

E

L

L

E

E

I

L

G

G

I

I

N

E

P

K

E

E

R

R

V

L

E

N

T

H

S

E

W

W

H

I

S

S

A

A

G

S

E

E

G

G

A

E

E

K

I

F

A

Q

K

N

D

T

I

M

D

K

E

D

F

F

V

Y

E

N

R

K

I

I

A

E

K

A

L

L

G

G

binding fe2/s2 (inorganic) cluster: C11 (= C652), C40 (= C681), S41 (= S682), C65 (= C706), C70 (= C711), H71 (= H712)

7bkbF Formate dehydrogenase - heterodisulfide reductase - formylmethanofuran dehydrogenase complex from methanospirillum hungatei (hexameric, composite structure) (see paper)
44% identity, 16% coverage: 644:769/777 of query aligns to 3:128/137 of 7bkbF

query
sites
7bkbF

E

K

P

P

L

Q

I

I

L

L

I

A

F

I

A

I

C
|
C

H

N

F
x
W

C

C

S

S

Y

Y

G

A

A

G

L

A

D

D

L

L

A

A

G

G

T

G

T

A

K

R

T

I

Q

Q

Y

Y

S

P

P

P

N

T

V

V

R

R

V

A

I

I

R

R

T

V

L

M

C
|
C

S
x
T

G

G

R

R

V

V

D

D

P

M

E

L

W

F

I

I

L

L

R

K

A

A

L

F

K

V

R

E

G

G

I

A

D

D

G

G

V

V

M

L

V

V

T

S

G

G

C
|
C

R

H

L

F

G

G

E

D

C
|
C

H
|
H

F

Y

R

L

V

E

G

G

N

N

Y

Y

H

K

A

A

V

A

D

K

R

R

I

M

K

F

A

M

L

I

K

K

K

N

L

L

E

R

E

N

I

I

G

G

I

L

N

D

P

D

E

R

R

R

V

F

E

R

T

M

S

T

W

F

H

V

S

S

A

A

G

S

E

E

G

G

A

A

E

K

I

W

A

G

K

M

D

V

I

M

D

E

E

D

F

V

V

T

E

N

R

T

I

I

A

K

K

E

L

L

G

G

binding fe2/s2 (inorganic) cluster: C11 (= C652), W13 (≠ F654), C40 (= C681), T41 (≠ S682), C65 (= C706), C70 (= C711), H71 (= H712)

6fahE Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
54% identity, 6% coverage: 571:620/777 of query aligns to 2:50/393 of 6fahE

query
sites
6fahE

V

V

N

I

E

E

K

K

C
|
C

I

I

G

G

C
|
C

R

S

I

K

C
|
C

E

Q

E

K

V

S

C
|
C

N
x
P

F

F

N

D

A

A

V

I

T

T

F

I

E

E

N

N

K

K

K

I

A

A

K

V

I

I

D

G

P

-

N

D

A

A

C
|
C

V
x
T

M
x
N

C
|
C

G

G

V

T

C
|
C

A

I

A

D

S

V

C
|
C

P

P

A

T

D

E

A
|
A

I

I

binding iron/sulfur cluster: C7 (= C576), C10 (= C579), C13 (= C582), C17 (= C586), P18 (≠ N587), C35 (= C605), T36 (≠ V606), N37 (≠ M607), C38 (= C608), C41 (= C611), C45 (= C615), A49 (= A619)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 180, 200, 281, 282, 307, 308, 320, 321, 322, 323, 324, 337, 338, 339, 343, 344, 358, 359, 377

8b6fAL ndutt15 (see paper)
31% identity, 12% coverage: 557:647/777 of query aligns to 101:206/218 of 8b6fAL

query
sites
8b6fAL

I

L

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

T

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G

A

C
|
C

R
x
K

I

L

C
|
C

E

Q

E

S

V

A

C
|
C

N

P

F

A

N

R

A

A

V
x
I

T

T

F

I

E

E

N

T

K

E

-

P

-

R

-

P

-

D

-

G

-

S

-

R

-

T

-

V

K

R

A
x
Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V

F

C
|
C

A

Q

A

E

S

A

C
|
C

P
|
P

A

V

D

D

A
|
A

I
|
I

D

V

L
x
E

G

G

-

P

-

N

-

Y

-

E

-

Q

-

T

-

A

F

Y

F

L

K

H

E

E

D

D

A

L

I

F

V

Y

A

D

M

K

I

Y

D

K

A

L

L

L

A

E

E

N

E

G

K

D

K

K

V

W

E

E

P

P

L

Q

I

I

binding iron/sulfur cluster: H106 (≠ A562), C118 (= C576), I119 (= I577), C121 (= C579), K122 (≠ R580), C124 (= C582), C128 (= C586), I133 (≠ V591), Y150 (≠ A598), C157 (= C605), I158 (≠ V606), Y159 (≠ M607), C160 (= C608), G161 (= G609), C163 (= C611), C167 (= C615), P168 (= P616), A171 (= A619), I172 (= I620), E174 (≠ L622)

7zm7I Cryoem structure of mitochondrial complex i from chaetomium thermophilum (inhibited by ddm) (see paper)
38% identity, 8% coverage: 558:620/777 of query aligns to 70:140/185 of 7zm7I

query
sites
7zm7I

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G
x
A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

V

C
|
C

N
x
P

F

A

N

Q

A

A

V
x
I

T

T

F

I

E

E

-

A

-

E

-

R

-

A

-

D

-

G

-

S

-

R

N

T

K

T

K

R

A
x
Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G

G

V

F

C
|
C

A

Q

A

E

S

S

C
|
C

P

P

A

V

D

D

A
|
A

I
|
I

binding iron/sulfur cluster: H74 (≠ A562), C86 (= C576), I87 (= I577), A88 (≠ G578), C89 (= C579), K90 (≠ R580), C92 (= C582), C96 (= C586), P97 (≠ N587), I101 (≠ V591), Y118 (≠ A598), C125 (= C605), I126 (≠ V606), Y127 (≠ M607), C128 (= C608), C131 (= C611), C135 (= C615), A139 (= A619), I140 (= I620)

5gupH structure of mammalian respiratory supercomplex I1III2IV1 (see paper)
37% identity, 8% coverage: 558:620/777 of query aligns to 61:131/176 of 5gupH

query
sites
5gupH

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G
x
A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

V

C
|
C

N

P

F

A

N

Q

A

A

V

I

T

T

F

I

E

E

-

A

-

E

-

P

-

R

-

A

-

D

-

G

-

S

-

R

N

T

K

T

K
x
R

A

Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V
x
F

C
|
C

A

Q

A

E

S

A

C
|
C

P
|
P

A

V

D

D

A
|
A

I
|
I

binding iron/sulfur cluster: H65 (≠ A562), C77 (= C576), I78 (= I577), A79 (≠ G578), C80 (= C579), K81 (≠ R580), C83 (= C582), C87 (= C586), C116 (= C605), I117 (≠ V606), Y118 (≠ M607), C119 (= C608), G120 (= G609), F121 (≠ V610), C122 (= C611), C126 (= C615), P127 (= P616), A130 (= A619), I131 (= I620)
binding : R108 (≠ K597)

Sites not aligning to the query:

8e73S8 qcr9 (see paper)
36% identity, 8% coverage: 558:620/777 of query aligns to 66:136/181 of 8e73S8

query
sites
8e73S8

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

-

Y

-

V

T

N

G

E

E

K

R

C
|
C

I
|
I

G

A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

I

C
|
C

N

P

F

A

N

Q

A

A

V
x
I

T

T

F

I

E

E

-

A

-

E

-

R

-

E

-

D

-

G

-

S

-

R

N

T

K

T

K

R

A

Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V
x
F

C
|
C

A

Q

A

E

S

A

C
|
C

P
|
P

A

V

D

D

A
|
A

I
|
I

binding iron/sulfur cluster: H70 (≠ A562), C82 (= C576), I83 (= I577), C85 (= C579), K86 (≠ R580), C88 (= C582), C92 (= C586), I97 (≠ V591), C121 (= C605), I122 (≠ V606), Y123 (≠ M607), C124 (= C608), G125 (= G609), F126 (≠ V610), C127 (= C611), C131 (= C615), P132 (= P616), A135 (= A619), I136 (= I620)

Sites not aligning to the query:

binding iron/sulfur cluster: 138

Q9VF27 NADH dehydrogenase (ubiquinone) 23 kDa subunit; EC 7.1.1.2 from Drosophila melanogaster (Fruit fly) (see paper)
32% identity, 13% coverage: 518:620/777 of query aligns to 61:172/217 of Q9VF27

query
sites
Q9VF27

R

R

P

A

A

A

E

S

T

T

D

M

V

F

K

F

G

G

I

E

F

L

L

L

A

R

G

G

A

F

A

A

S

V

G

T

P

L

K

A

D

H

I

I

Q

F

D

K

S

E

V

P

A

A

S

T

A

I

G

N

L

Y

A

P

A

F

S

E

K

K

A

G

-

P

M

L

E

S

L

P

I

R

F

F

G

R

G

G

E

E

A

H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C

C

I

I

G

A

C

C

R

K

I

L

C

C

E

E

E

A

V

I

C

C

N

P

F

A

N

Q

A

A

V

I

T

T

F

I

E

E

-

A

-

E

-

R

-

A

-

D

-

G

-

S

-

R

N

T

K

T

K

R

A

Y

K

D

I

I

D

D

P

M

N

T

A

K

C

C

V

I

M

Y

C

C

G

G

V

F

C

C

A

Q

A

E

S

A

C

C

P

P

A

V

D

D

A

A

I

I

Sites not aligning to the query:

199 G→D: Disrupts mitochondrial function and results in enlarged mitochondria. Neurons present vacuolar lesions leading to neurodegeneration in the central brain. Results in behavioral defects and shorten lifespan.

7b0nI 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM. (see paper)
37% identity, 8% coverage: 558:620/777 of query aligns to 76:146/191 of 7b0nI

query
sites
7b0nI

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G
x
A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

I

C
|
C

N

P

F

A

N

L

A

A

V

I

T

T

F

I

E

D

-

A

-

E

-

R

-

I

-

D

-

G

-

S

-

R

N

T

K

T

K

K

A

Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V

Y

C
|
C

A

Q

A

E

S

S

C
|
C

P

P

A

V

D

D

A

A

I

I

binding iron/sulfur cluster: H80 (≠ A562), C92 (= C576), I93 (= I577), A94 (≠ G578), C95 (= C579), K96 (≠ R580), C98 (= C582), C102 (= C586), C131 (= C605), I132 (≠ V606), Y133 (≠ M607), C134 (= C608), G135 (= G609), C137 (= C611), C141 (= C615)

Sites not aligning to the query:

binding iron/sulfur cluster: 148

7arcI Cryo-em structure of polytomella complex-i (peripheral arm) (see paper)
36% identity, 8% coverage: 558:620/777 of query aligns to 84:154/199 of 7arcI

query
sites
7arcI

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

-

Y

-

V

T

N

G

E

E

K

R

C
|
C

I
|
I

G
x
S

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

I

C
|
C

N
x
P

F

A

N

Q

A

A

V
x
I

T

T

F

I

E

E

-

A

-

E

-

R

-

E

-

D

-

G

-

S

-

R

-

K

N

T

K

T

K
x
R

A
x
Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V
x
F

C
|
C

A

Q

A

E

S

A

C
|
C

P

P

A

V

D

D

A
|
A

I
|
I

binding iron/sulfur cluster: H88 (≠ A562), C100 (= C576), I101 (= I577), S102 (≠ G578), C103 (= C579), K104 (≠ R580), C106 (= C582), C110 (= C586), P111 (≠ N587), I115 (≠ V591), Y132 (≠ A598), C139 (= C605), I140 (≠ V606), Y141 (≠ M607), C142 (= C608), G143 (= G609), F144 (≠ V610), C145 (= C611), C149 (= C615), A153 (= A619), I154 (= I620)
binding : R131 (≠ K597)

Sites not aligning to the query:

binding iron/sulfur cluster: 156
binding : 168, 169, 171, 172, 173

8b9zI Drosophila melanogaster complex i in the active state (dm1) (see paper)
32% identity, 13% coverage: 518:620/777 of query aligns to 30:141/186 of 8b9zI

query
sites
8b9zI

R

R

P

A

A

A

E

S

T

T

D

M

V

F

K

F

G

G

I

E

F

L

L

L

A

R

G

G

A

F

A

A

S

V

G

T

P

L

K

A

D

H

I

I

Q

F

D

K

S

E

V

P

A

A

S

T

A

I

G

N

L

Y

A

P

A

F

S

E

K

K

A

G

-

P

M

L

E

S

L

P

I

R

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G
x
A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

I

C
|
C

N
x
P

F

A

N

Q

A

A

V

I

T

T

F

I

E

E

-

A

-

E

-

R

-

A

-

D

-

G

-

S

-

R

N

T

K

T

K

R

A
x
Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V
x
F

C
|
C

A

Q

A

E

S

A

C
|
C

P

P

A

V

D

D

A
|
A

I
|
I

binding iron/sulfur cluster: H75 (≠ A562), C87 (= C576), I88 (= I577), A89 (≠ G578), C90 (= C579), K91 (≠ R580), C93 (= C582), C97 (= C586), P98 (≠ N587), Y119 (≠ A598), C126 (= C605), I127 (≠ V606), Y128 (≠ M607), C129 (= C608), G130 (= G609), F131 (≠ V610), C132 (= C611), C136 (= C615), A140 (= A619), I141 (= I620)

Sites not aligning to the query:

binding iron/sulfur cluster: 143

7ak5I Cryo-em structure of respiratory complex i in the deactive state from mus musculus at 3.2 a (see paper)
36% identity, 8% coverage: 558:620/777 of query aligns to 63:133/178 of 7ak5I

query
sites
7ak5I

F

F

G

R

G

G

E

E

A
x
H

E

A

F

L

D

R

P

R

Y

Y

N

P

A

S

Y

-

V

-

N

G

E

E

K

R

C
|
C

I
|
I

G
x
A

C
|
C

R
x
K

I

L

C
|
C

E

E

E

A

V

I

C
|
C

N

P

F

A

N

Q

A

A

V

I

T

T

F

I

E

E

-

A

-

E

-

P

-

R

-

A

-

D

-

G

-

S

-

R

N

T

K

T

K

R

A

Y

K

D

I

I

D

D

P

M

N

T

A

K

C
|
C

V
x
I

M
x
Y

C
|
C

G
|
G

V

F

C
|
C

A

Q

A

E

S

A

C
|
C

P

P

A

V

D

D

A

A

I
|
I

binding iron/sulfur cluster: H67 (≠ A562), C79 (= C576), I80 (= I577), A81 (≠ G578), C82 (= C579), K83 (≠ R580), C85 (= C582), C89 (= C586), C118 (= C605), I119 (≠ V606), Y120 (≠ M607), C121 (= C608), G122 (= G609), C124 (= C611), C128 (= C615), I133 (= I620)

Sites not aligning to the query:

binding iron/sulfur cluster: 135

7np8B Crystal structure of the coenzyme f420-dependent sulfite reductase from methanocaldococcus jannaschii at 2.3-a resolution (see paper)
45% identity, 6% coverage: 571:619/777 of query aligns to 490:538/620 of 7np8B

query
sites
7np8B

V

V

N

N

E

E

K

K

C
|
C

I
x
N

G
|
G

C
|
C

R
x
G

I

R

C
|
C

E

A

E

E

V

V

C
|
C

N
x
K

F

V

N

E

A

A

V
x
I

T

D

F

I

E

R

N

G

K

E

K

T

A
x
S

K

Y

I

T

D

N

P

Y

N

N

A

V

C
|
C

V
|
V

M
x
G

C
|
C

G
|
G

V

K

C
|
C

A

I

A

K

S

N

C
|
C

P

P

A
x
N

D

E

A
|
A

binding iron/sulfur cluster: C495 (= C576), N496 (≠ I577), G497 (= G578), C498 (= C579), G499 (≠ R580), C501 (= C582), C505 (= C586), I510 (≠ V591), S517 (≠ A598), C524 (= C605), V525 (= V606), G526 (≠ M607), C527 (= C608), G528 (= G609), C530 (= C611), C534 (= C615), N536 (≠ A617), A538 (= A619)
binding siroheme: K506 (≠ N587)

Sites not aligning to the query:

binding calcium ion: 85, 228, 273
binding flavin-adenine dinucleotide: 83, 84, 85, 87, 88, 89, 90, 107, 108, 129, 130, 131, 132, 133, 134, 137, 156, 199, 200, 202, 271, 272, 273, 281
binding iron/sulfur cluster: 6, 15, 16, 17, 18, 19, 21, 25, 26, 29, 30, 36, 42, 44, 46, 48, 50, 54, 55, 159, 160, 180, 201, 202, 203, 204, 256, 259, 315, 428, 434, 436, 437, 467, 468, 470, 472, 488, 539
binding sulfite ion: 355, 423, 460, 462
binding siroheme: 355, 384, 386, 387, 388, 392, 429, 434, 459, 460, 462, 470, 472, 474, 553, 554, 555, 556, 594

8a6tB Cryo-em structure of the electron bifurcating fe-fe hydrogenase hydabc complex from thermoanaerobacter kivui in the reduced state (see paper)
45% identity, 6% coverage: 571:620/777 of query aligns to 577:627/630 of 8a6tB

query
sites
8a6tB

V
x
I

N

D

E

P

E

G

K

K

C
|
C

I
|
I

G

G

C
|
C

R

G

I

K

C
|
C

E

V

E

K

V

V

C
|
C

N

P

F

V

N

G

A
|
A

V
x
I

T

S

F

G

E

E

N

K

K

K

A

P

K

H

-

V

I
|
I

D

D

P

Q

N

S

A

K

C
|
C

V

I

M

K

C

C

G

G

V

A

C
|
C

A

A

A

E

S

N

C
|
C

P

P

A
x
K

D

G

A
|
A

I
|
I

binding iron/sulfur cluster: I577 (≠ V571), C582 (= C576), I583 (= I577), C585 (= C579), C588 (= C582), C592 (= C586), A596 (= A590), I597 (≠ V591), I607 (= I600), C612 (= C605), C618 (= C611), C622 (= C615), K624 (≠ A617), A626 (= A619), I627 (= I620)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 36, 82, 85, 86
binding flavin mononucleotide: 201, 227, 230, 355, 535, 536
binding nadp nicotinamide-adenine-dinucleotide phosphate: 320, 337, 340, 341, 342, 433
binding iron/sulfur cluster: 487, 488, 489, 491, 494, 534, 536, 537, 575
binding zinc ion: 471, 558, 564

7p8nB Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
44% identity, 7% coverage: 571:621/777 of query aligns to 562:613/613 of 7p8nB

query
sites
7p8nB

V

I

N

N

E

P

E

D

K

I

C
|
C

I

K

G

G

C
|
C

R
x
G

I

L

C
|
C

E

A

E

R

V

S

C
|
C

N

P

F

Q

N

N

A

A

V

I

T

T

F

G

E

E

N

R

K

G

K

K

A

P

-
x
Y

K

T

I
|
I

D

D

P

Q

N

E

A

K

C
|
C

V

V

M
x
K

C
|
C

G
|
G

V

L

C
|
C

A

A

A

S

S

K

C
|
C

P

P

A

F

D

K

A

A

I

I

D

E

binding iron/sulfur cluster: C567 (= C576), C570 (= C579), G571 (≠ R580), C573 (= C582), C577 (= C586), Y590 (vs. gap), I592 (= I600), C597 (= C605), K599 (≠ M607), C600 (= C608), G601 (= G609), C603 (= C611), C607 (= C615)

Sites not aligning to the query:

binding fe2/s2 (inorganic) cluster: 31, 33, 34, 36, 67, 68, 69, 70, 71
binding flavin mononucleotide: 185, 186, 187, 213, 215, 216, 217, 301, 304, 305, 306, 340, 341, 521, 522
binding iron/sulfur cluster: 320, 473, 474, 475, 476, 477, 480, 519, 520, 522, 523, 560
binding zinc ion: 457, 544, 549, 554

7npaA Crystal structure of the coenzyme f420-dependent sulfite reductase from methanothermococcus thermolithotrophicus at 1.55-a resolution (see paper)
38% identity, 7% coverage: 571:622/777 of query aligns to 490:541/618 of 7npaA

query
sites
7npaA

V

V

N

N

E

E

K

N

C
|
C

I
x
N

G
|
G

C
|
C

R
x
G

I

R

C
|
C

E

A

E

E

V

V

C
|
C

N
x
K

F
x
I

N

E

A
|
A

V
x
I

T

D

F

I

E

R

N

G

K

E

K

T

A
x
S

K

Y

I

T

D

N

P

Y

N

N

A

V

C
|
C

V
x
I

M
x
G

C
|
C

G
|
G

V

K

C
|
C

A

I

A

K

S

A

C
|
C

P

P

A
x
N

D

E

A
x
G

I
x
R

D

D

L

V

binding iron/sulfur cluster: C495 (= C576), N496 (≠ I577), G497 (= G578), C498 (= C579), G499 (≠ R580), C501 (= C582), C505 (= C586), I507 (≠ F588), A509 (= A590), I510 (≠ V591), S517 (≠ A598), C524 (= C605), I525 (≠ V606), G526 (≠ M607), C527 (= C608), G528 (= G609), C530 (= C611), C534 (= C615), N536 (≠ A617), G538 (≠ A619), R539 (≠ I620)
binding siroheme: K506 (≠ N587)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 83, 84, 85, 87, 88, 89, 90, 107, 108, 129, 131, 132, 133, 134, 137, 156, 158, 199, 200, 201, 202, 272, 273, 274, 281
binding iron/sulfur cluster: 15, 16, 17, 18, 19, 21, 25, 30, 36, 42, 44, 46, 48, 50, 54, 55, 159, 160, 180, 201, 202, 203, 204, 256, 259, 315, 428, 434, 436, 437, 467, 468, 470, 472, 488
binding siroheme: 353, 355, 384, 386, 387, 388, 392, 394, 428, 429, 434, 459, 460, 462, 470, 472, 474, 553, 554, 555, 556, 594

Query Sequence

>WP_012964674.1 NCBI__GCF_000025505.1:WP_012964674.1
MRIGVFVCHCGLNIARIVDVQEVVNYAKNLEDVYFSTDLKYACSDSGQEEIIKAIKENRL
DAVVIAACSPKLHEHTFRKAAMRAGLNPYMVLMANIREQCSWVHQEHPKAATEKAKDLVR
MAVAAARKLEPLSRKRIEVKKSAVVIGGGVAGIEAALTLANAGVKVYLIEKAPTIGGKMA
TLNEVFPTNDCSICILAPKMSEAFNHENIEVITNAEVLEVSGHVGNFKVKVRKHPRYVDE
NKCKGCIDDCSSVCPVEVPNEFDYTIGVRKAIYLPIPQSTPLYAAIDWEHCIGCRLCEKA
CEPKAVDFNQKPEDLEIEAGVIIVATGYKPFDARRKEEYGYGVYKNVITTLELERLLSAS
GPTLGNLYRPSDSSVPRKIAFIQCVGSRDVKTNKYCSRVCCMVSIKNAYIIKERYPEADV
SVFFIDIRAFGRMYEEFFARAQEKGIRFIRGRVAEIYELENGNLILTYENTLTGEIKEEE
FELVVLSIGMEGNTDLANKLGISVGEDGFYDVAHPKLRPAETDVKGIFLAGAASGPKDIQ
DSVASAGLAASKAMELIFGGEAEFDPYNAYVNEEKCIGCRICEEVCNFNAVTFENKKAKI
DPNACVMCGVCAASCPADAIDLGFFKEDAIVAMIDALAEEKKVEPLILIFACHFCSYGAL
DLAGTTKTQYSPNVRVIRTLCSGRVDPEWILRALKRGIDGVMVTGCRLGECHFRVGNYHA
VDRIKALKKLLEEIGINPERVETSWHSAGEGAEIAKDIDEFVERIAKLGSIYEEVAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory