SitesBLAST

R12 (= R5) to Q: in ARGINSA; 18-fold reduction in catalytic efficiency toward argininosuccinate; dbSNP:rs145138923
D31 (= D24) to N: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs754995756
K51 (= K44) mutation to N: 2-fold reduction in activity.
K69 (≠ E62) modified: N6-acetyllysine
E73 (vs. gap) to K: in ARGINSA; complete loss of argininosuccinate lyase activity; abolishes protein expression
D87 (= D75) to G: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs752100894
H89 (= H77) mutation to Q: 10-fold reduction in activity.
R94 (≠ S82) to C: in ARGINSA; severe; dbSNP:rs374304304
R95 (≠ K83) to C: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs28940585
R113 (= R101) to Q: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on nitric oxide production; dbSNP:rs752783461
D120 (≠ C108) to E: in ARGINSA; severe
V178 (≠ D166) to M: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941473
T181 (≠ R169) to S: in a breast cancer sample; somatic mutation
R182 (= R170) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs751590073
R186 (= R174) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs752397242
G200 (= G188) to V: in a breast cancer sample; somatic mutation
R236 (= R224) to W: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on NOS complex formation; dbSNP:rs761268464
D237 (= D225) to N: in ARGINSA; severe; dbSNP:rs552951774
Q286 (= Q273) to R: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941472
K288 (= K275) modified: N6-acetyllysine; mutation to R: Refractory to inhibition by TSA and NAM and by addition of extra amino acids. No effect on protein structure.
R297 (= R284) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs750431938
R306 (≠ Y293) to W: in ARGINSA; severe; dbSNP:rs868834862
Q326 (= Q313) to L: in ARGINSA; severe
V335 (≠ S322) to L: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression
M360 (= M347) to T: in ARGINSA; loss of argininosuccinate lyase activity; may cause protein misfolding; dbSNP:rs875989948
M382 (≠ V370) to R: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression
R385 (= R373) to L: in ARGINSA; severe
H388 (= H376) to Q: in ARGINSA; severe
A398 (≠ N386) to D: in ARGINSA; impairs tetramer formation likely due to protein misfolding; loss of argininosuccinate lyase activity

Sites not aligning to the query:

456 R → W: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs759396688

P24058 Argininosuccinate lyase; ASAL; Arginosuccinase; Delta crystallin II; Delta-2 crystallin; EC 4.3.2.1 from Anas platyrhynchos (Mallard) (Anas boschas) (see 4 papers)
37% identity, 91% coverage: 5:443/481 of query aligns to 14:456/468 of P24058

query
sites
P24058

R

R

L

F

S

S

G

G

S

S

L

T

D

D

E

P

D

I

A

M

L

E

K

K

L

L

T

N

S

S

S
|
S

L

I

E

A

H

Y

D
|
D

R

Q

H

R

I

L

F

S

K

E

Y

V

D

D

I

I

L

Q

V

G

D

S

Y

M

A

A

H

Y

V

A

L

K

G

A

L

L

L

E

K

K

V

A

G

G

L

I

I

L

T

T

K

K

E

T

D

E

A

L

K

E

R

K

I

I

F

L

E

S

A

G

L

L

R

E

E

K

V

I

E

S

K

E

-

E

-

W

-

S

-

K

-

G

L

V

G

F

L

V

E

V

R

K

L

Q

S

S

G

D

E

E

D
|
D

V

I

H

H

E

T

A

A

I

N

E

E

S

R

K

R

V

L

V

K

E

E

I

L

A

I

K

G

E

D

S

I

G

A

M

G

K

K

M

L

H

H

T

T

A

G

R

R

S

S

R

R

N
|
N

D
|
D

E

Q

I

V

A

V

A

T

C

D

L

L

R

K

L

L

F

F

A

M

R

K

D

N

K

S

I

L

I

S

E

I

I

I

L

S

S

T

E

H

L

L

E

Q

L

L

R

I

K

K

T

T

F

L

I

V

E

E

K

R

A

A

R

A

Q

I

Y

E

F

I

D

D

C

V

I

I

M

L

P

P

G

G

F

Y

T
|
T

H
|
H

L

L

Q

Q

Y

K

A

A

Q

Q

P

P

T

I

K

R

L

W

S

S

H

Q

Y

F

L

L

I

L

A

S

Y

H

H

A

D

V

A

A

I

L

R

T

R

R

D

D

F

S

D

E

R

R

G

L

I

G

E

E

I

V

F

K

S

K

R

R

V

I

N

N

K

V

S

L

P

P

L

L

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

N

P

P

H

L

E

D

L

I

D

D

R

R

E

E

Y

M

V

L

A

R

Q

S

L

E

L

L

G

E

F

F

D

A

G

S

L

I

V

S

E

L

N

N

T

S

M

M

D

D

A

A

S

I

A

S

S

E

R
|
R

D

D

F

F

L

V

I

V

E

E

S

F

L

L

F

S

L

F

S

A

T

T

S

L

I

L

M

M

L

I

N

H

L

L

S

S

R

K

F

M

C

A

E

E

E

D

I

L

I

I

L

I

W

Y

A

S

-

T

S

S

E

E

F

F

D

G

F

F

V

L

E

T

L

L

P

S

D

D

E

A

F

F

S

S

S
x
T

S

G

S
|
S

S

S

I

L

M

M

P

P

Q

Q

K

K

N
|
N

P

P

D
|
D

V

S

A

L

E
|
E

I

L

I

I

R

R

A

S

K

K

A

A

G

G

K

R

S

V

V

F

G

G

Y

R

L

L

A

A

S

S

A

I

C

L

T

M

I

V

L

L

K

K

A

G

L

L

P

P

Y

S

A

T

Y
|
Y

N

N

R
x
K

D

D

F

L

Q
|
Q

E

E

M
x
D

N
x
K

S

E

L

A

L

V

Y

F

V

D

S

V

M

V

N

D

E

T

C

L

L

T

L

A

S

V

V

L

K

Q

T

V

F

A

R

T

K

G

M

V

F

I

S

S

A

T

L

L

K

Q

F

I

K

S

E

K

E

E

V

N

M

M

R

-

E

E

K

K

A

A

S

L

K

T

G

P

L

E

T

M

L

L

A

A

T

T

D

D

L

L

A

A

D

L

L

Y

L

L

V

V

R

R

K

K

G

G

V

V

P

P

F

F

R

R

L

Q

S

A

H

H

K

T

I

A

V

S

G

G

S

K

L

A

V

V

K

H

K

L

N

-

R

-

I

-

P

-

S

-

A

-

E

-

L

-

I

-

K

-

E

-

A

A

E

E

S

T

F

K

G

G

V

I

K

T

I

I

-

N

E

K

V

L

S

S

E

L

D

E

E

D

L

L

K

K

-

S

-

I

-

S

-

P

-

Q

-

F

-

S

-

D

-

V

-

S

E

Q

W

V

L

F

D

N

V

F

E

V

K

N

S

S

L

V

E

E

R

Q

R

Y

K

T

N

A

V

L

G

A

G

G

T

T

A

A

K

K

E

S

L

V

E

T

R

T

M

Q

I

I

R

E

E

Q

S29 (= S20) binding in chain A; mutation to A: 10% decrease in catalytic efficiency.
D33 (= D24) mutation to N: 99% decrease in catalytic efficiency.
D89 (= D75) mutation to N: Loss of activity.
N116 (= N102) binding in chain A; mutation to D: 99% decrease in catalytic efficiency.
D117 (= D103) mutation to A: 55% decrease in catalytic efficiency.; mutation to E: 58% decrease in catalytic efficiency.
T161 (= T147) binding in chain C; mutation to A: Loss of activity.; mutation to D: Loss of activity.; mutation to S: 30% decrease in catalytic efficiency.; mutation to V: Loss of activity.
H162 (= H148) mutation to E: Loss of activity.
R238 (= R224) mutation to Q: Loss of activity.
T281 (≠ S266) mutation to V: 80% decrease in catalytic efficiency.
S283 (= S268) mutation to A: Loss of activity.; mutation to C: Loss of activity.; mutation to D: Loss of activity.; mutation to H: Loss of activity.; mutation to T: Loss of activity.
N291 (= N276) binding in chain B; mutation to L: Loss of activity.
D293 (= D278) mutation to N: 99% decrease in catalytic efficiency.
E296 (= E281) mutation to D: Loss of activity.
Y323 (= Y308) binding in chain A
K325 (≠ R310) mutation to N: 99% decrease in catalytic efficiency.
Q328 (= Q313) binding in chain A
D330 (≠ M315) mutation to N: Loss of activity.
K331 (≠ N316) binding in chain A; mutation to Q: Loss of activity.

Sites not aligning to the query:

11 W→A: 98% decrease in catalytic efficiency.; W→F: 90% decrease in catalytic efficiency.; W→M: 99% decrease in catalytic efficiency.; W→R: 97% decrease in catalytic efficiency.; W→Y: 50% decrease in catalytic efficiency.

1k7wD Crystal structure of s283a duck delta 2 crystallin mutant (see paper)
37% identity, 89% coverage: 16:443/481 of query aligns to 8:439/450 of 1k7wD

query
sites
1k7wD

K

K

L

L

T

N

S

S

L

I

E

A

H

Y

D

D

R

Q

H

R

I

L

F

S

K

E

Y

V

D

D

I

I

L

Q

V

G

D

S

Y

M

A

A

H

Y

V

A

L

K

G

A

L

L

L

E

K

K

V

A

G

G

L

I

I

L

T

T

K

K

E

T

D

E

A

L

K

E

R

K

I

I

F

L

E

S

A

G

L

L

R

E

E

K

V

I

E

S

K

E

-

E

-

W

-

S

-

K

-

G

L

V

G

F

L

V

E

V

R

K

L

Q

S

S

G

D

E
|
E

D

D

V

I

H

H

E

T

A

A

I

N

E

E

S

R

K

R

V

L

V

K

E

E

I

L

A

I

K

G

E

D

S

I

G

A

M

G

K

K

M

L

H

H

T

T

A

G

R

R

S

S

R
|
R

N
|
N

D

D

E

Q

I
x
V

A

V

A

T

C

D

L

L

R

K

L

L

F

F

A

M

R

K

D

N

K

S

I

L

I

S

E

I

I

I

L

S

S

T

E

H

L

L

E

Q

L

L

R

I

K

K

T

T

F

L

I

V

E

E

K

R

A

A

R

A

Q

I

Y

E

F

I

D

D

C

V

I

I

M

L

P

P

G

G

F

Y

T
|
T

H
|
H

L

L

Q

Q

Y

K

A

A

Q

Q

P

P

T

I

K

R

L

W

S

S

H

Q

Y

F

L

L

I

L

A

S

Y

H

H

A

D

V

A

A

I

L

R

T

R

R

D

D

F

S

D

E

R

R

G

L

I

G

E

E

I

V

F

K

S

K

R

R

V

I

N

N

K

V

S

L

P

P

L

L

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

N

P

P

H

L

E

D

L

I

D

D

R

R

E

E

Y

M

V

L

A

R

Q

S

L

E

L

L

G

E

F

F

D

A

G

S

L

I

V

S

E

L

N

N

T

S

M

M

D

D

A

A

S

I

A

S

S

E

R

R

D

D

F

F

L

V

I

V

E

E

S

F

L

L

F

S

L

F

S

A

T

T

S

L

I

L

M

M

L

I

N

H

L

L

S

S

R

K

F

M

C

A

E

E

E

D

I

L

I

I

L

I

W

Y

A

S

-

T

S

S

E

E

F

F

D

G

F

F

V

L

E

T

L

L

P

S

D

D

E

A

F

F

S

S

S

T

S

G

S
x
A

S
|
S

I

L

M

M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

V

S

A

L

E
|
E

I

L

I

I

R

R

A

S

K

K

A

A

G

G

K

R

S

V

V

F

G

G

Y

R

L

L

A

A

S

S

A

I

C

L

T

M

I

V

L

L

K

K

A

G

L

L

P

P

Y

S

A

T

Y
|
Y

N

N

R

K

D

D

F

L

Q
|
Q

E

E

M

D

N
x
K

S

E

L

A

L

V

Y

F

V

D

S

V

M

V

N

D

E

T

C

L

L

T

L

A

S

V

V

L

K

Q

T

V

F

A

R

T

K

G

M

V

F

I

S

S

A

T

L

L

K

Q

F

I

K

S

E

K

E

E

V

N

M

M

R

-

E

E

K

K

A

A

S

L

K

T

G

P

L

E

T

M

L

L

A

A

T

T

D

D

L

L

A

A

D

L

L

Y

L

L

V

V

R

R

K

K

G

G

V

V

P

P

F

F

R

R

L

Q

S

A

H

H

K

T

I

A

V

S

G

G

S

K

L

A

V

V

K

H

K

L

N

-

R

-

I

-

P

-

S

-

A

-

E

-

L

-

I

-

K

-

E

-

A

A

E

E

S

T

F

K

G

G

V

I

K

T

I

I

-

N

E

K

V

L

S

S

E

L

D

E

E

D

L

L

K

K

-

S

-

I

-

S

-

P

-

Q

-

F

-

S

-

D

-

V

-

S

E

Q

W

V

L

F

D

N

V

F

E

V

K

N

S

S

L

V

E

E

R

Q

R

Y

K

T

N

A

V

L

G

G

T

T

A

A

K

K

E

S

L

V

E

T

R

T

M

Q

I

I

R

E

E

Q

active site: E71 (= E74), T144 (= T147), H145 (= H148), A266 (≠ S268), S267 (= S269), K272 (= K274), E279 (= E281)
binding argininosuccinate: R98 (= R101), N99 (= N102), V102 (≠ I105), T144 (= T147), H145 (= H148), Y306 (= Y308), Q311 (= Q313), K314 (≠ N316)

1tj7B Structure determination and refinement at 2.44 a resolution of argininosuccinate lyase from e. Coli (see paper)
35% identity, 91% coverage: 5:441/481 of query aligns to 1:443/451 of 1tj7B

query
sites
1tj7B

R

R

L

F

S

T

G

Q

S

A

L

A

D

D

E

Q

D

R

A

F

L

K

K

Q

L

F

T

N

S

D

S

S

L

L

E

R

H

F

D

D

R

Y

H

R

I

L

F

A

K

E

Y

Q

D

D

I

I

L

V

V

G

D

S

Y

V

A

A

H

W

V

S

L

K

G

A

L

L

L

V

K

T

V

V

G

G

L

V

I

L

T

T

K

A

E

E

D

E

A

Q

K

A

R

Q

I

L

F

E

E

E

A

A

L

L

-

N

-

V

-

L

-

E

-

D

-

V

R

R

E

A

V

R

E

P

K

Q

L

Q

G

I

L

L

E

E

R

S

L

-

S

D

G

A

E

E

D

D

V

I

H

H

E

S

A

W

I

V

E

E

S

G

K

K

V

L

V

I

E

D

I

K

A

V

K

G

E

Q

S

L

G

G

M

K

K

K

M

L

H

H

T

T

A

G

R

R

S

S

R

R

N

N

D

D

E

Q

I

V

A

A

A

T

C

D

L

L

R

K

L

L

F

W

A

C

R

K

D

D

K

T

I

V

I

S

E

E

I

L

L

L

S

T

E

A

L

N

L

R

E

Q

L

L

R

Q

K

S

T

A

F

L

I

V

E

E

K

T

A

A

R

Q

Q

N

Y

N

F

Q

D

D

C

A

I

V

M

M

P

P

G

G

F

Y

T

T

H
|
H

L

L

Q

Q

Y

R

A

A

Q

Q

P

P

T

V

K

T

L

F

S

A

H

H

Y

W

L

C

I

L

A

A

Y

Y

H

V

D

E

A

M

I

L

R

A

R

R

D

D

F

E

D

S

R

R

G

L

I

Q

E

D

I

A

F

L

S

K

R

R

V

L

N

D

K

V

S

S

P

P

L

L

G

G

A

C

A

G

A

A

F

L

A

A

S

G

T

T

P

A

H

Y

E

E

L

I

D

D

R

R

E

E

Y

Q

V

L

A

A

Q

G

L

W

L

L

G

G

F

F

D

A

G

S

L

A

V

T

E

R

N

N

T

S

M

L

D

D

A

S

S

V

A

S

S

D

R

R

D

D

F

H

L

V

I

L

E

E

S

L

L

L

F

S

L

A

S

A

T

A

S

I

I

G

M

M

L

V

N

H

L

L

S

S

R

R

F

F

C

A

E

E

E

D

I

L

I

I

L

F

W

F

-

N

A

T

S

G

E

E

F

A

D

G

F

F

V

V

E

E

L

L

P

S

D

D

E

R

F

V

S

T

S

S

S

G

S
|
S

S

S

I

L

M
|
M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

V

A

A

L

E
|
E

I

L

I

I

R

R

A

G

K

K

A

C

G

G

K

R

S

V

V

Q

G

G

Y

A

L

L

A

T

S

G

A

M

C

M

T

M

I

T

L

L

K

K

A

G

L

L

P

P

Y

L

A

A

Y

Y

N

N

R

K

D

D

F

M

Q

Q

E

E

M

D

N

K

S

E

L

G

L

L

Y

F

V

D

S

A

M

L

N

D

E

T

C

W

L

L

L

D

S

C

V

L

K

H

T

M

F

A

R

A

K

L

M

V

F

L

S

D

A

G

L

I

K

Q

F

V

K

K

E

R

E

P

V

R

M

C

R

Q

E

E

K

A

A

A

S

Q

K

Q

G

G

L

Y

T

A

L

N

A

A

T

T

D

E

L

L

A

A

D

D

L

Y

L

L

V

V

R

A

K

K

G

G

V

V

P

P

F

F

R

R

L

E

S

A

H

H

K

H

I

I

V

V

G

G

-

E

S

A

L

V

V

V

K

E

K

A

N

I

R

R

I

Q

P

G

P

K

S

P

A

L

E

E

E

D

L

L

-

P

I

L

K

S

E

E

A

L

E

Q

S

K

F

F

G

S

V

-

K

-

I

-

E

Q

V

V

S

I

E

D

D

E

E

D

L

V

K

Y

E

P

W

I

L

L

D

S

V

L

E

Q

K

S

S

C

L

L

E

D

R

K

R

R

K

A

N

A

V

K

G

G

T

V

A

S

K

P

E

Q

L

V

E

A

R

Q

M

A

I

I

active site: H150 (= H148), S271 (= S268), K277 (= K274), E284 (= E281)
binding phosphate ion: S271 (= S268), M274 (= M271), K277 (= K274)

1hy0A Crystal structure of wild type duck delta 1 crystallin (eye lens protein) (see paper)
35% identity, 89% coverage: 17:443/481 of query aligns to 7:437/447 of 1hy0A

query
sites
1hy0A

L

L

T

S

S

T

S

S

L

I

E

S

H

T

D

E

R

Q

H

R

I

L

F

S

K

E

Y

V

D

D

I

I

L

Q

V

A

D

S

Y

I

A

A

H

Y

V

A

L

K

G

A

L

L

L

E

K

K

V

A

G

G

L

I

I

L

T

T

K

K

E

T

D

E

A

L

K

E

R

K

I

I

F

L

E

S

A

G

L

L

R

E

E

K

V

I

-

S

E

E

K

E

L

L

G

S

-

K

-

G

-

V

-

I

L

V

E

V

R

T

L

Q

S

S

G

D

E
|
E

D

D

V

I

H

Q

E

T

A

A

I

N

E

E

S

R

K

R

V

L

V

K

E

E

I

L

A

I

K

G

E

D

S

I

G

A

M

G

K

K

M

L

H

H

T

T

A

G

R

R

S

S

R

R

N

N

D

E

E

Q

I

V

A

V

A

T

C

D

L

L

R

K

L

L

F

F

A

M

R

K

D

N

K

S

I

L

I

S

E

I

I

I

L

S

S

T

E

H

L

L

E

Q

L

L

R

I

K

K

T

T

F

L

I

V

E

E

K

R

A

A

R

A

Q

I

Y

E

F

I

D

D

C

V

I

I

M

L

P

P

G

G

F

Y

T
|
T

H
|
H

L

L

Q

Q

Y

K

A

A

Q

Q

P

P

T

I

K

R

L

W

S

S

H

Q

Y

F

L

L

I

L

A

S

Y

H

H

A

D

V

A

A

I

L

R

T

R

R

D

D

F

S

D

E

R

R

G

L

I

G

E

E

I

V

F

K

S

K

R

R

V

I

N

N

K

V

S

L

P

P

L

L

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

N

P

P

H

L

E

D

L

I

D

D

R

R

E

E

Y

M

V

L

A

R

Q

S

L

E

L

L

G

E

F

F

D

A

G

S

L

I

V

S

E

L

N

N

T

S

M

M

D

D

A

A

S

I

A

S

S

E

R

R

D

D

F

F

L

V

I

V

E

E

S

F

L

L

F

S

L

V

S

A

T

T

S

L

I

L

M

L

L

I

N

H

L

L

S

S

R

K

F

M

C

A

E

E

E

D

I

L

I

I

L

I

W

Y

A

S

-

T

S

S

E

E

F

F

D

G

F

F

V

L

E

T

L

L

P

S

D

D

E

A

F

F

S

S

S

T

S

G

S
|
S

I

L

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

A

L

E
|
E

I

L

I

I

R

R

A

S

K

K

A

S

G

G

K

R

S

V

V

F

G

G

Y

R

L

L

A

A

S

S

A

I

C

L

T

M

I

V

L

L

K

K

A

G

L

L

P

P

Y

S

A

T

Y

Y

N

N

R

K

D

D

F

L

Q

Q

E

E

M

D

N

K

S

E

L

A

L

V

Y

I

V

D

S

V

M

V

N

D

E

T

C

L

L

T

L

A

S

V

V

L

K

Q

T

V

F

A

R

T

K

G

M

V

F

I

S

S

A

T

L

L

K

Q

F

I

K

S

E

K

E

E

V

N

M

M

R

-

E

E

K

K

A

A

S

L

K

T

G

P

L

E

T

M

L

L

A

A

T

T

D

D

L

L

A

A

D

L

L

Y

L

L

V

V

R

R

K

K

G

G

V

M

P

P

F

F

R

R

L

Q

S

A

H

H

K

T

I

A

V

S

G

G

S

K

L

A

V

V

K

H

K

L

N

-

R

-

I

-

P

-

S

-

A

-

E

-

L

-

I

-

K

-

E

-

A

A

E

E

S

T

F

K

G

G

V

I

K

A

I

I

-

N

E

N

V

L

S

T

E

L

D

E

E

D

L

L

K

K

-

S

-

I

-

S

-

P

-

L

-

F

-

S

-

D

-

V

-

S

E

Q

W

V

L

F

D

N

V

F

E

V

K

N

S

S

L

V

E

E

R

Q

R

Y

K

T

N

A

V

L

G

G

T

T

A

A

K

K

E

S

L

V

E

T

R

T

M

Q

I

I

R

E

E

Q

active site: E69 (= E74), T142 (= T147), H143 (= H148), S264 (= S268), S265 (= S269), K270 (= K274), E277 (= E281)
binding sulfate ion: S264 (= S268), M267 (= M271), N272 (= N276)

6ienB Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
37% identity, 92% coverage: 13:456/481 of query aligns to 3:449/454 of 6ienB

query
sites
6ienB

D

D

A

A

L

L

K

A

-

A

L

L

T

S

S

K

S

S

L

T

E

H

H

F

D

D

R

W

H

V

I

L

F

A

K

P

Y

Y

D

D

I

L

L

T

V

A

D

S

Y

R

A

A

H

H

V

T

L

M

G

V

L

L

L

F

K

R

V

A

G

G

L

L

I

L

T

T

K

E

E

E

D

Q

A

R

K

D

R

G

I

L

F

L

E

A

A

G

L

L

-

D

-

S

-

L

-

A

R

Q

E

D

V

V

E

A

K

D

L

G

G

S

L

F

E

G

R

P

L

L

-

V

S

T

G

D

E

E

D

D

V

V

H

H

E

A

A

A

I

L

E

E

S

R

K

G

V

L

V

I

E

D

-

R

I

V

A

G

K

P

E

D

S

L

G

G

M

G

K

R

M

L

H

R

T

A

A

G

R

R

S
|
S

R
|
R

N
|
N

D

D

E

Q

I

V

A

A

C

L

L

F

R

R

L

M

F

W

A

L

R

R

D

D

K

A

I

V

I

R

E

R

I

V

L

A

S

T

E

G

L

V

L

L

E

D

L

V

R

V

K

G

T

A

F

L

I

A

E

E

K

Q

A

A

R

A

Q

A

Y

H

F

P

D

S

C

A

I

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

Y

S

A

A

Q

Q

P

P

T

I

K

L

L

L

S

A

H

H

Y

H

L

L

I

L

A

A

Y

H

H

A

D

H

A

P

I

L

R

L

R

R

D

D

F

L

D

D

R

R

G

I

I

V

E

D

I

F

F

D

S

K

R

R

V

A

N

A

K

V

S

S

P

P

L

Y

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

S

P

S

H

L

E

G

L

L

D

D

R

P

E

D

Y

A

V

I

A

A

Q

A

L

D

L

L

G

G

F

F

D

S

G

A

L

A

V

A

E

D

N

N

T

S

M

V

D

D

A

A

S

T

A

A

S

A

R

R

D

D

F

F

L

A

I

A

E

E

S

A

L

A

F

F

L

V

S

F

T

A

S

M

I

I

M

A

L

V

N

D

L

L

S

S

R

R

F

L

C

A

E

E

E

D

I

I

L

V

W

W

A

S

S

S

-

T

E

E

F

F

D

G

F

Y

V

V

E

T

L

L

P

H

D

D

E

S

F

W

S

S

S

T

S

G

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

V

I

A

A

E

E

I

L

I

A

R

R

A

G

K

K

A

S

G

G

K

R

S

L

V

I

G

G

Y

N

L

L

A

A

S

G

A

L

C

L

T

A

I

T

L

L

K

K

A

A

L

Q

P

P

Y

L

A

A

Y
|
Y

N

N

R

R

D

D

F

L

Q
|
Q

E

E

M

D

N
x
K

S

E

L

P

L

V

Y

F

V

D

S

S

M

V

N

A

E

Q

C

L

L

E

L

L

S

L

V

L

K

P

T

A

F

M

R

A

K

G

M

L

F

V

S

A

A

S

L

L

K

T

F

F

K

N

E

V

E

Q

V

R

M

M

R

A

E

E

K

L

A

A

S

P

K

A

G

G

L

Y

T

T

L

L

A

A

T

T

D

D

L

L

A

A

D

E

L

W

L

L

V

V

R

R

K

Q

G

G

V

V

P

P

F

F

R

R

L

S

S

A

H

H

K

E

I

A

V

A

G

G

S

A

L

A

V

V

K

R

K

A

N

A

R

E

I

Q

P

R

P

G

S

V

A

G

E

L

E

Q

L

E

I

L

K

T

E

D

A

D

E

E

S

L

F

A

G

A

V

I

K

S

I

P

E

E

V

L

S

T

E

P

D

-

E

Q

L

V

K

R

E

E

W

V

L

L

D

T

V

I

E

E

K

G

S

S

L

V

E

S

R

A

R

R

K

D

N

C

V

R

G

G

T

T

A

A

K

P

E

G

E

R

L

-

E

-

R

-

M

-

I

V

R

A

E

E

R

Q

E

L

R

N

K

A

I

I

S

G

E

E

D

A

E

A

E

E

K

R

L

L

K

R

binding argininosuccinate: S97 (= S100), R98 (= R101), N99 (= N102), T144 (= T147), H145 (= H148), S266 (= S268), S267 (= S269), M269 (= M271), K272 (= K274), Y306 (= Y308), Q311 (= Q313), K314 (≠ N316)

6ienC Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
39% identity, 76% coverage: 13:379/481 of query aligns to 3:377/418 of 6ienC

query
sites
6ienC

D

D

A

A

L

L

K

A

-

A

L

L

T

S

S

K

S

S

L

T

E

H

H

F

D

D

R

W

H

V

I

L

F

A

K

P

Y

Y

D

D

I

L

L

T

V

A

D

S

Y

R

A

A

H

H

V

T

L

M

G

V

L

L

L

F

K

R

V

A

G

G

L

L

I

L

T

T

K

E

E

E

D

Q

A

R

K

D

R

G

I

L

F

L

E

A

A

G

L

L

-

D

-

S

-

L

-

A

R

Q

E

D

V

V

E

A

K

D

L

G

G

S

L

F

E

G

R

P

L

L

-

V

S

T

G

D

E

E

D

D

V

V

H

H

E

A

A

A

I

L

E

E

S

R

K

G

V

L

V

I

E

D

-

R

I

V

A

G

K

P

E

D

S

L

G

G

M

G

K

R

M

L

H

R

T

A

A

G

R

R

S
|
S

R
|
R

N
|
N

D

D

E

Q

I
x
V

A

A

C

L

L

F

R

R

L

M

F

W

A

L

R

R

D

D

K

A

I

V

I

R

E

R

I

V

L

A

S

T

E

G

L

V

L

L

E

D

L

V

R

V

K

G

T

A

F

L

I

A

E

E

K

Q

A

A

R

A

Q

A

Y

H

F

P

D

S

C

A

I

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

Y

S

A

A

Q

Q

P

P

T

I

K

L

L

L

S

A

H

H

Y

H

L

L

I

L

A

A

Y

H

H

A

D

H

A

P

I

L

R

L

R

R

D

D

F

L

D

D

R

R

G

I

I

V

E

D

I

F

F

D

S

K

R

R

V

A

N

A

K

V

S

S

P

P

L

Y

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

S

P

S

H

L

E

G

L

L

D

D

R

P

E

D

Y

A

V

I

A

A

Q

A

L

D

L

L

G

G

F

F

D

S

G

A

L

A

V

A

E

D

N

N

T

S

M

V

D

D

A

A

S

T

A

A

S

A

R

R

D

D

F

F

L

A

I

A

E

E

S

A

L

A

F

F

L

V

S

F

T

A

S

M

I

I

M

A

L

V

N

D

L

L

S

S

R

R

F

L

C

A

E

E

E

D

I

I

L

V

W

W

A

S

S

S

-

T

E

E

F

F

D

G

F

Y

V

V

E

T

L

L

P

H

D

D

E

S

F

W

S

S

S

T

S

G

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N

N

P

P

D

D

V

I

A

A

E

E

I

L

I

A

R

R

A

G

K

K

A

S

G

G

K

R

S

L

V

I

G

G

Y

N

L

L

A

A

S

G

A

L

C

L

T

A

I

T

L

L

K

K

A

A

L

Q

P

P

Y

L

A

A

Y
|
Y

N

N

R

R

D

D

F

L

Q
|
Q

E

E

M

D

N
x
K

S

E

L

P

L

V

Y

F

V

D

S

S

M

V

N

A

E

Q

C

L

L

E

L

L

S

L

V

L

K

P

T

A

F

M

R

A

K

G

M

L

F

V

S

A

A

S

L

L

K

T

F

F

K

N

E

V

E

Q

V

R

M

M

R

A

E

E

K

L

A

A

S

P

K

A

G

G

L

Y

T

T

L

L

A

A

T

T

D

D

L

L

A

A

D

E

L

W

L

L

V

V

R

R

K

Q

G

G

V

V

P

P

F

F

R

R

L

S

S

A

H

H

K

R

I

E

V

V

binding arginine: R98 (= R101), N99 (= N102), V102 (≠ I105), Y306 (= Y308), Q311 (= Q313), K314 (≠ N316)
binding argininosuccinate: T144 (= T147), H145 (= H148), S266 (= S268), S267 (= S269), M269 (= M271), K272 (= K274)
binding fumaric acid: S97 (= S100), R98 (= R101), N99 (= N102)

P02521 Delta-1 crystallin; Delta crystallin I from Gallus gallus (Chicken) (see paper)
33% identity, 92% coverage: 1:443/481 of query aligns to 8:454/466 of P02521

query
sites
P02521

M

L

I

L

R

G

S

G

R

R

L

F

S

V

G

G

S

S

L

T

D

D

E

P

D

I

A

M

L

E

K

I

L

L

T

S

S

S

L

I

E

S

H

T

D

E

R

Q

H

R

I

L

F

T

K

E

Y

V

D

D

I

I

L

Q

V

A

D

S

Y

M

A

A

H

Y

V

A

L

K

G

A

L

L

L

E

K

K

V

A

G

S

L

I

I

L

T

T

K

K

E

T

D

E

A

L

K

E

R

K

I

I

F

L

E

S

A

G

L

L

R

E

E

K

V

I

E

S

K

E

-

E

-

S

-

K

-

G

L

V

G

L

L

V

E

M

R

T

L

Q

S

S

G

D

E

E

D

D

V

I

H

Q

E

T

A

A

I

I

E

E

S

R

K

R

V

L

V

K

E

E

I

L

A

I

K

G

E

D

S

I

G

A

M

G

K

K

M

L

H

Q

T

T

A

G

R

R

S

S

R

R

N

N

D

E

E

Q

I

V

A

V

A

T

C

D

L

L

R

K

L

L

F

L

A

L

R

K

D

S

K

S

I

I

I

S

E

V

I

I

L

S

S

T

E

H

L

L

E

Q

L

L

R

I

K

K

T

T

F

L

I

V

E

E

K

R

A

A

R

A

Q

I

Y

E

F

I

D

D

C

I

I

I

M

M

P

P

G

G

F

Y

T

T

H

H

L

L

Q

Q

Y

K

A

A

Q

L

P

P

T

I

K

R

L

W

S

S

H

Q

Y

F

L

L

I

L

A

S

Y

H

H

A

D

V

A

A

I

L

R

T

R

R

D

D

F

S

D

E

R

R

G

L

I

G

E

E

I

V

F

K

S

K

R

R

V

I

N

T

K

V

S

L

P

P

L

L

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

N

P

P

H

L

E

E

L

I

D

D

R

R

E

E

Y

L

V

L

A

R

Q

S

L

E

L

L

G

D

F

M

D

T

G

S

L

I

V

T

E

L

N

N

T

S

M

I

D

D

A

A

S

I

A

S

S

E

R

R

D

D

F

F

L

V

I

V

E

E

S

L

L

I

F

S

L

V

S

A

T

T

S

L

I

L

M

M

L

I

N

H

L

L

S

S

R

K

F

L

C

A

E

E

E

D

I

L

I

I

L

I

W

F

A

S

-

T

S

T

E

E

F

F

D

G

F

F

V

V

E

T

L

L

P

S

D

D

E

A

F

Y

S

S

S

T

S

G

S

S

I

L

M

L

P

P

Q

Q

K

K

N

N

P

P

D

D

V

S

A

L

E

E

I

L

I

I

R

R

A

S

K

K

A

A

G

G

K

R

S

V

V

F

G

G

Y

R

L

L

A

A

S

A

A

I

C

L

T

M

I

V

L

L

K

K

A

G

L

I

P

P

Y

S

A

T

Y

F

N

S

R

K

D

D

F

L

Q

Q

E

E

M

D

N

K

S

E

L

A

L

V

Y

L

V

D

S

V

M

V

N

D

E

T

C

L

L

T

L

A

S

V

V

L

K

Q

T

V

F

A

R

T

K

G

M

V

F

I

S

S

A

T

L

L

K

Q

F

V

K

N

E

K

E

E

V

N

M

M

R

-

E

E

K

K

A

A

S

L

K

T

G

P

L

E

T

L

L

L

A

S

T

T

D

D

L

L

A

A

D

L

L

Y

L

L

V

V

R

R

K

K

G

G

V

M

P

P

F

I

R

R

L

Q

S

A

H

Q

K

T

I

A

V

S

G

G

S

K

L

A

V

V

K

H

K

L

N

-

R

-

I

-

P

-

S

-

A

-

E

-

L

-

I

-

K

-

E

-

A

A

E

E

S

T

F

K

G

G

V

I

K

T

I

I

-

N

E

N

V

L

S

T

E

L

D

E

E

D

L

L

K

K

-

S

-

I

-

S

-

P

-

L

-

F

-

A

-

S

-

D

-

V

-

S

E

Q

W

V

L

F

D

S

V

V

E

V

K

N

S

S

L

V

E

E

R

Q

R

Y

K

T

N

A

V

V

G

G

T

T

A

A

K

K

E

S

L

V

E

T

R

A

M

Q

I

I

R

E

E

Q

Sites not aligning to the query:

2 modified: Blocked amino end (Ala)

6ienA Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
36% identity, 92% coverage: 13:456/481 of query aligns to 3:447/452 of 6ienA

query
sites
6ienA

D

D

A

A

L

L

K

A

-

A

L

L

T

S

S

K

S

S

L

T

E

H

H

F

D

D

R

W

H

V

I

L

F

A

K

P

Y

Y

D

D

I

L

L

T

V

A

D

S

Y

R

A

A

H

H

V

T

L

M

G

V

L

L

L

F

K

R

V

A

G

G

L

L

I

L

T

T

K

E

E

E

D

Q

A

R

K

D

R

G

I

L

F

L

E

A

A

G

L

L

-

D

-

S

-

L

-

A

R

Q

E

D

V

V

E

A

K

D

L

G

G

S

L

F

E

G

R

P

L

L

-

V

S

T

G

D

E

E

D

D

V

V

H

H

E

A

A

A

I

L

E

E

S

R

K

G

V

L

V

I

E

D

-

R

I

V

A

G

K

P

E

D

S

L

G

G

M

G

K

R

M

L

H

R

T

A

A

G

R

R

S

S

R
|
R

N
|
N

D

D

E

Q

I
x
V

A

A

C

L

L

F

R

R

L

M

F

W

A

L

R

R

D

D

K

A

I

V

I

R

E

R

I

V

L

A

S

T

E

G

L

V

L

L

E

D

L

V

R

V

K

G

T

A

F

L

I

A

E

E

K

Q

A

A

R

A

Q

A

Y

H

F

P

D

S

C

A

I

I

M

M

P

P

G

G

F

K

T
|
T

H
|
H

L

L

Q

Q

Y

S

A

A

Q

Q

P

P

T

I

K

L

L

L

S

A

H

H

Y

H

L

L

I

L

A

A

Y

H

H

A

D

H

A

P

I

L

R

L

R

R

D

D

F

L

D

D

R

R

G

I

I

V

E

D

I

F

F

D

S

K

R

R

V

A

N

A

K

V

S

S

P

P

L

Y

G

G

A

S

A

G

A

A

F

L

A

A

S

G

T

S

P

S

H

L

E

G

L

L

D

D

R

P

E

D

Y

A

V

I

A

A

Q

A

L

D

L

L

G

G

F

F

D

S

G

A

L

A

V

A

E

D

N

N

T

S

M

V

D

D

A

A

S

T

A

A

S

A

R

R

D

D

F

F

L

A

I

A

E

E

S

A

L

A

F

F

L

V

S

F

T

A

S

M

I

I

M

A

L

V

N

D

L

L

S

S

R

R

F

L

C

A

E

E

E

D

I

I

L

V

W

W

A

S

S

S

-

T

E

E

F

F

D

G

F

Y

V

V

E

T

L

L

P

H

D

D

E

S

F

W

S

S

S

T

S

G

S
|
S

I

-

M

-

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

I

A

A

E

E

I

L

I

A

R

R

A

G

K

K

A

S

G

G

K

R

S

L

V

I

G

G

Y

N

L

L

A

A

S

G

A

L

C

L

T

A

I

T

L

L

K

K

A

A

L

Q

P

P

Y

L

A

A

Y
|
Y

N

N

R

R

D

D

F

L

Q
|
Q

E

E

M

D

N
x
K

S

E

L

P

L

V

Y

F

V

D

S

S

M

V

N

A

E

Q

C

L

L

E

L

L

S

L

V

L

K

P

T

A

F

M

R

A

K

G

M

L

F

V

S

A

A

S

L

L

K

T

F

F

K

N

E

V

E

Q

V

R

M

M

R

A

E

E

K

L

A

A

S

P

K

A

G

G

L

Y

T

T

L

L

A

A

T

T

D

D

L

L

A

A

D

E

L

W

L

L

V

V

R

R

K

Q

G

G

V

V

P

P

F

F

R

R

L

S

S

A

H

H

K

E

I

A

V

A

G

G

S

A

L

A

V

V

K

R

K

A

N

A

R

E

I

Q

P

R

P

G

S

V

A

G

E

L

E

Q

L

E

I

L

K

T

E

D

A

D

E

E

S

L

F

A

G

A

V

I

K

S

I

P

E

E

V

L

S

T

E

P

D

-

E

Q

L

V

K

R

E

E

W

V

L

L

D

T

V

I

E

E

K

G

S

S

L

V

E

S

R

A

R

R

K

D

N

C

V

R

G

G

T

T

A

A

K

P

E

G

E

R

L

-

E

-

R

-

M

-

I

V

R

A

E

E

R

Q

E

L

R

N

K

A

I

I

S

G

E

E

D

A

E

A

E

E

K

R

L

L

K

R

binding argininosuccinate: R98 (= R101), N99 (= N102), V102 (≠ I105), T144 (= T147), H145 (= H148), Y304 (= Y308), Q309 (= Q313), K312 (≠ N316)
binding fumaric acid: S266 (= S268), S267 (= S269), K270 (= K274), N272 (= N276)

6g3hA Crystal structure of edds lyase in complex with ss-edds (see paper)
32% identity, 97% coverage: 10:477/481 of query aligns to 24:490/497 of 6g3hA

query
sites
6g3hA

L

F

D

D

E

A

D

A

A

A

L

L

K

T

L

L

T

T

S

S

S

A

L

-

E

-

H

-

D

-

R

-

H

-

I

V

F

F

K

P

Y

Y

D

D

I

S

L

Q

V

I

D

H

Y

R

A

A

H

H

V

V

L

V

G

M

L

L

L

T

K

E

V

Q

G

G

L

I

I

L

T

T

K

V

E

E

D

E

A

S

K

A

R

T

I

I

F

L

E

S

A

G

L

L

R

A

E

Q

V

V

E

D

K

E

L

L

G

A

L

A

E

T

R

D

L

G

S

S

G

L

E

R

D

-

V

T

H

Y

E

L

A

P

I

Y

E

E

S

A

K

A

V

L

V

K

E

R

I

T

A

I

K

G

E

S

S

V

G

A

M

G

K

K

M

M

H

H

T

I

A

G

R

R

S

S

R

R

N

N

D

D

E

L

I

A

A

N

A

A

C

G

L

K

R

R

L

M

F

F

A

L

R

R

D

D

K

Q

I

L

E

R

I

T

L

I

S

E

E

A

L

V

L

I

E

G

L

Y

R

R

K

E

T

A

F

V

I

V

E

H

K

K

A

A

R

A

Q

D

Y

H

F

L

D

D

C

T

I

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

Y

E

A

A

Q

Q

P

P

T

I

K

T

L

L

S

G

H

H

Y

Y

L

L

I

M

A

A

Y

I

H

S

D

E

A

N

I

L

R

A

R

K

D

N

F

L

D

D

R

R

G

Y

I

R

E

E

I

L

F

Y

S

A

R

R

V

I

N

N

K

L

S

C

P

P

L

L

G

G

A

A

F

T

A

A

S

G

T

T

P

G

H

W

E

P

L

L

D

N

R

R

E

D

Y

R

V

T

A

S

Q

A

L

L

G

G

F

F

D

D

G

G

L

L

V

V

E

V

N

N

T

S

M

I

D

E

A

G

S

V

A

A

S

G

R

W

D

D

F

H

L

V

I

A

E

E

S

H

L

A

F

F

L

V

S

N

T

A

S

V

I

F

M

L

L

S

N

G

L

L

S

S

R

R

F

L

C

A

E

S

E

E

I

I

I

Q

L

L

W

W

A

S

S

T

-

D

E

E

F

Y

D

Q

F

V

V

A

E

E

L

L

P

D

D

A

E

S

F

F

S

A

S

G

S

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

V

S

A

L

E

E

I
x
R

I

S

R

R

A

K

K

A

A

A

G

F

K

A

S

A

V

M

G

G

Y

P

L

L

A

V

S

G

A

I

C

L

T

T

I

S

L

L

K

N

A

A

L

I

P

E

Y

Y

A

Q

Y

Y

N

S

R

A

D

A

F

R

Q

V

E

E

M

L

N

E

S

P

L

R

L

-

Y

-

V

-

S

S

M

I

N

D

E

A

C

L

L

I

L

A

S

A

V

T

K

H

T

A

F

M

R

T

K

G

M

V

F

V

S

R

A

T

L

L

K

H

F

P

K

N

E

K

E

E

V

R

M

M

R

R

E

Q

K

Y

A

A

S

A

K

E

G

N

L

Y

T

S

L

T

A

M

T

T

D

D

L

L

A

T

D

D

L

M

L

L

V

V

R

R

K

R

-

V

G

G

V

I

P

D

F

Y

R

R

L

E

S

A

H

H

K

E

I

I

V

V

G

A

S

H

L

V

V

V

-

I

-

T

-

A

-

I

-

E

-

K

-

G

-

I

K

K

K

A

N

N

R

K

I

I

P

-

S

-

A

G

E

L

E

D

L

L

I

V

K

Q

E

E

A

A

-

A

-

V

E

A

S

Q

F

T

G

G

V

A

K

G

I

I

E

N

V

V

S

S

E

A

D

D

E

D

L

I

K

K

E

D

W

A

L

L

D

D

V

P

E

W

K

Q

S

N

L

V

E

L

R

R

K

E

N

G

V

K

G

G

G

M

T

P

A

A

K

P

E

M

E

S

L

V

E

K

R

A

M

S

I

I

R

D

E

D

R

A

E

M

R

A

K

E

I

L

S

H

E

K

D

D

E

R

E

A

K

W

L

L

K

A

V

N

L

A

K

T

S

Q

R

A

I

L

E

A

E

N

R

A

I

K

K

Q

H

T

L

L

M

A

N

D

E

S

V

V

E

Q

R

Q

V

I

C

I

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: S276 (= S268), S277 (= S269), M279 (= M271), K282 (= K274), N284 (= N276), D286 (= D278), R290 (≠ I282)

Sites not aligning to the query:

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: 22

6g3gA Crystal structure of edds lyase in complex with succinate (see paper)
32% identity, 97% coverage: 10:477/481 of query aligns to 24:490/497 of 6g3gA

query
sites
6g3gA

L

F

D

D

E

A

D

A

A

A

L

L

K

T

L

L

T

T

S

S

S

A

L

-

E

-

H

-

D

-

R

-

H

-

I

V

F

F

K

P

Y

Y

D

D

I

S

L

Q

V

I

D

H

Y

R

A

A

H

H

V

V

L

V

G

M

L

L

L

T

K

E

V

Q

G

G

L

I

I

L

T

T

K

V

E

E

D

E

A

S

K

A

R

T

I

I

F

L

E

S

A

G

L

L

R

A

E

Q

V

V

E

D

K

E

L

L

G

A

L

A

E

T

R

D

L

G

S

S

G

L

E

R

D

-

V

T

H

Y

E

L

A

P

I

Y

E

E

S

A

K

A

V

L

V

K

E

R

I

T

A

I

K

G

E

S

S

V

G

A

M

G

K

K

M

M

H

H

T

I

A

G

R

R

S

S

R

R

N

N

D

D

E

L

I

A

A

N

A

A

C

G

L

K

R

R

L

M

F

F

A

L

R

R

D

D

K

Q

I

L

E

R

I

T

L

I

S

E

E

A

L

V

L

I

E

G

L

Y

R

R

K

E

T

A

F

V

I

V

E

H

K

K

A

A

R

A

Q

D

Y

H

F

L

D

D

C

T

I

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

Y

E

A

A

Q

Q

P

P

T

I

K

T

L

L

S

G

H

H

Y

Y

L

L

I

M

A

A

Y

I

H

S

D

E

A

N

I

L

R

A

R

K

D

N

F

L

D

D

R

R

G

Y

I

R

E

E

I

L

F

Y

S

A

R

R

V

I

N

N

K

L

S

C

P

P

L

L

G

G

A

A

F

T

A

A

S

G

T

T

P

G

H

W

E

P

L

L

D

N

R

R

E

D

Y

R

V

T

A

S

Q

A

L

L

G

G

F

F

D

D

G

G

L

L

V

V

E

V

N

N

T

S

M

I

D

E

A

G

S

V

A

A

S

G

R

W

D

D

F

H

L

V

I

A

E

E

S

H

L

A

F

F

L

V

S

N

T

A

S

V

I

F

M

L

L

S

N

G

L

L

S

S

R

R

F

L

C

A

E

S

E

E

I

I

I

Q

L

L

W

W

A

S

S

T

-

D

E

E

F

Y

D

Q

F

V

V

A

E

E

L

L

P

D

D

A

E

S

F

F

S

A

S

G

S

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

A

L

E

E

I

R

I

S

R

R

A

K

K

A

A

A

G

F

K

A

S

A

V

M

G

G

Y

P

L

L

A

V

S

G

A

I

C

L

T

T

I

S

L

L

K

N

A

A

L

I

P

E

Y

Y

A

Q

Y

Y

N

S

R

A

D

A

F

R

Q

V

E

E

M

L

N

E

S

P

L

R

L

-

Y

-

V

-

S

S

M

I

N

D

E

A

C

L

L

I

L

A

S

A

V

T

K

H

T

A

F

M

R

T

K

G

M

V

F

V

S

R

A

T

L

L

K

H

F

P

K

N

E

K

E

E

V

R

M

M

R

R

E

Q

K

Y

A

A

S

A

K

E

G

N

L

Y

T

S

L

T

A

M

T

T

D

D

L

L

A

T

D

D

L

M

L

L

V

V

R

R

K

R

-

V

G

G

V

I

P

D

F

Y

R

R

L

E

S

A

H

H

K

E

I

I

V

V

G

A

S

H

L

V

V

V

-

I

-

T

-

A

-

I

-

E

-

K

-

G

-

I

K

K

K

A

N

N

R

K

I

I

P

-

S

-

A

G

E

L

E

D

L

L

I

V

K

Q

E

E

A

A

-

A

-

V

E

A

S

Q

F

T

G

G

V

A

K

G

I

I

E

N

V

V

S

S

E

A

D

D

E

D

L

I

K

K

E

D

W

A

L

L

D

D

V

P

E

W

K

Q

S

N

L

V

E

L

R

R

K

E

N

G

V

K

G

G

G

M

T

P

A

A

K

P

E

M

E

S

L

V

E

K

R

A

M

S

I

I

R

D

E

D

R

A

E

M

R

A

K

E

I

L

S

H

E

K

D

D

E

R

E

A

K

W

L

L

K

A

V

N

L

A

K

T

S

Q

R

A

I

L

E

A

E

N

R

A

I

K

K

Q

H

T

L

L

M

A

N

D

E

S

V

V

E

Q

R

Q

V

I

C

I

binding succinic acid: S276 (= S268), S277 (= S269), M279 (= M271), K282 (= K274), N284 (= N276)

6g3fA Crystal structure of edds lyase in complex with fumarate (see paper)
32% identity, 97% coverage: 10:477/481 of query aligns to 24:490/497 of 6g3fA

query
sites
6g3fA

L

F

D

D

E

A

D

A

A

A

L

L

K

T

L

L

T

T

S

S

S

A

L

-

E

-

H

-

D

-

R

-

H

-

I

V

F

F

K

P

Y

Y

D

D

I

S

L

Q

V

I

D

H

Y

R

A

A

H

H

V

V

L

V

G

M

L

L

L

T

K

E

V

Q

G

G

L

I

I

L

T

T

K

V

E

E

D

E

A

S

K

A

R

T

I

I

F

L

E

S

A

G

L

L

R

A

E

Q

V

V

E

D

K

E

L

L

G

A

L

A

E

T

R

D

L

G

S

S

G

L

E

R

D

-

V

T

H

Y

E

L

A

P

I

Y

E

E

S

A

K

A

V

L

V

K

E

R

I

T

A

I

K

G

E

S

S

V

G

A

M

G

K

K

M

M

H

H

T

I

A

G

R

R

S

S

R

R

N

N

D

D

E

L

I

A

A

N

A

A

C

G

L

K

R

R

L

M

F

F

A

L

R

R

D

D

K

Q

I

L

E

R

I

T

L

I

S

E

E

A

L

V

L

I

E

G

L

Y

R

R

K

E

T

A

F

V

I

V

E

H

K

K

A

A

R

A

Q

D

Y

H

F

L

D

D

C

T

I

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

Y

E

A

A

Q

Q

P

P

T

I

K

T

L

L

S

G

H

H

Y

Y

L

L

I

M

A

A

Y

I

H

S

D

E

A

N

I

L

R

A

R

K

D

N

F

L

D

D

R

R

G

Y

I

R

E

E

I

L

F

Y

S

A

R

R

V

I

N

N

K

L

S

C

P

P

L

L

G

G

A

A

F

T

A

A

S

G

T

T

P

G

H

W

E

P

L

L

D

N

R

R

E

D

Y

R

V

T

A

S

Q

A

L

L

G

G

F

F

D

D

G

G

L

L

V

V

E

V

N

N

T

S

M

I

D

E

A

G

S

V

A

A

S

G

R

W

D

D

F

H

L

V

I

A

E

E

S

H

L

A

F

F

L

V

S

N

T

A

S

V

I

F

M

L

L

S

N

G

L

L

S

S

R

R

F

L

C

A

E

S

E

E

I

I

I

Q

L

L

W

W

A

S

S

T

-

D

E

E

F

Y

D

Q

F

V

V

A

E

E

L

L

P

D

D

A

E

S

F

F

S

A

S

G

S

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

A

L

E

E

I

R

I

S

R

R

A

K

K

A

A

A

G

F

K

A

S

A

V

M

G

G

Y

P

L

L

A

V

S

G

A

I

C

L

T

T

I

S

L

L

K

N

A

A

L

I

P

E

Y

Y

A

Q

Y

Y

N

S

R

A

D

A

F

R

Q

V

E

E

M

L

N

E

S

P

L

R

L

-

Y

-

V

-

S

S

M

I

N

D

E

A

C

L

L

I

L

A

S

A

V

T

K

H

T

A

F

M

R

T

K

G

M

V

F

V

S

R

A

T

L

L

K

H

F

P

K

N

E

K

E

E

V

R

M

M

R

R

E

Q

K

Y

A

A

S

A

K

E

G

N

L

Y

T

S

L

T

A

M

T

T

D

D

L

L

A

T

D

D

L

M

L

L

V

V

R

R

K

R

-

V

G

G

V

I

P

D

F

Y

R

R

L

E

S

A

H

H

K

E

I

I

V

V

G

A

S

H

L

V

V

V

-

I

-

T

-

A

-

I

-

E

-

K

-

G

-

I

K

K

K

A

N

N

R

K

I

I

P

-

S

-

A

G

E

L

E

D

L

L

I

V

K

Q

E

E

A

A

-

A

-

V

E

A

S

Q

F

T

G

G

V

A

K

G

I

I

E

N

V

V

S

S

E

A

D

D

E

D

L

I

K

K

E

D

W

A

L

L

D

D

V

P

E

W

K

Q

S

N

L

V

E

L

R

R

K

E

N

G

V

K

G

G

G

M

T

P

A

A

K

P

E

M

E

S

L

V

E

K

R

A

M

S

I

I

R

D

E

D

R

A

E

M

R

A

K

E

I

L

S

H

E

K

D

D

E

R

E

A

K

W

L

L

K

A

V

N

L

A

K

T

S

Q

R

A

I

L

E

A

E

N

R

A

I

K

K

Q

H

T

L

L

M

A

N

D

E

S

V

V

E

Q

R

Q

V

I

C

I

binding fumaric acid: S276 (= S268), S277 (= S269), M279 (= M271), K282 (= K274), N284 (= N276)

6g3iA Crystal structure of edds lyase in complex with n-(2-aminoethyl) aspartic acid (aeaa) (see paper)
32% identity, 97% coverage: 10:477/481 of query aligns to 24:490/496 of 6g3iA

query
sites
6g3iA

L

F

D

D

E

A

D

A

A

A

L

L

K

T

L

L

T

T

S

S

S

A

L

-

E

-

H

-

D

-

R

-

H

-

I

V

F

F

K

P

Y

Y

D

D

I

S

L

Q

V

I

D

H

Y

R

A

A

H

H

V

V

L

V

G

M

L

L

L

T

K

E

V

Q

G

G

L

I

I

L

T

T

K

V

E

E

D

E

A

S

K

A

R

T

I

I

F

L

E

S

A

G

L

L

R

A

E

Q

V

V

E

D

K

E

L

L

G

A

L

A

E

T

R

D

L

G

S

S

G

L

E

R

D

-

V

T

H

Y

E

L

A

P

I

Y

E

E

S

A

K

A

V

L

V

K

E

R

I

T

A

I

K

G

E

S

S

V

G

A

M

G

K

K

M

M

H

H

T

I

A

G

R

R

S

S

R

R

N

N

D

D

E

L

I

A

A

N

A

A

C

G

L

K

R

R

L

M

F

F

A

L

R

R

D

D

K

Q

I

L

E

R

I

T

L

I

S

E

E

A

L

V

L

I

E

G

L

Y

R

R

K

E

T

A

F

V

I

V

E

H

K

K

A

A

R

A

Q

D

Y

H

F

L

D

D

C

T

I

V

M

M

P

V

G

V

F

Y

T

T

H

Q

L

R

Q

K

Y

E

A

A

Q

Q

P

P

T

I

K

T

L

L

S

G

H

H

Y

Y

L

L

I

M

A

A

Y

I

H

S

D

E

A

N

I

L

R

A

R

K

D

N

F

L

D

D

R

R

G

Y

I

R

E

E

I

L

F

Y

S

A

R

R

V

I

N

N

K

L

S

C

P

P

L

L

G

G

A

A

F

T

A

A

S

G

T

T

P

G

H

W

E

P

L

L

D

N

R

R

E

D

Y

R

V

T

A

S

Q

A

L

L

G

G

F

F

D

D

G

G

L

L

V

V

E

V

N

N

T

S

M

I

D

E

A

G

S

V

A

A

S

G

R

W

D

D

F

H

L

V

I

A

E

E

S

H

L

A

F

F

L

V

S

N

T

A

S

V

I

F

M

L

L

S

N

G

L

L

S

S

R

R

F

L

C

A

E

S

E

E

I

I

I

Q

L

L

W

W

A

S

S

T

-

D

E

E

F

Y

D

Q

F

V

V

A

E

E

L

L

P

D

D

A

E

S

F

F

S

A

S

G

S

T

S
|
S

I

I

M
|
M

P

P

Q

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

V

S

A

L

E

E

I
x
R

I

S

R

R

A

K

K

A

A

A

G

F

K

A

S

A

V

M

G

G

Y

P

L

L

A

V

S

G

A

I

C

L

T

T

I

S

L

L

K

N

A

A

L

I

P

E

Y

Y

A

Q

Y

Y

N

S

R

A

D

A

F

R

Q

V

E

E

M

L

N

E

S

P

L

R

L

-

Y

-

V

-

S

S

M

I

N

D

E

A

C

L

L

I

L

A

S

A

V

T

K

H

T

A

F

M

R

T

K

G

M

V

F

V

S

R

A

T

L

L

K

H

F

P

K

N

E

K

E

E

V

R

M

M

R

R

E

Q

K

Y

A

A

S

A

K

E

G

N

L

Y

T

S

L

T

A

M

T

T

D

D

L

L

A

T

D

D

L

M

L

L

V

V

R

R

K

R

-

V

G

G

V

I

P

D

F

Y

R

R

L

E

S

A

H

H

K

E

I

I

V

V

G

A

S

H

L

V

V

V

-

I

-

T

-

A

-

I

-

E

-

K

-

G

-

I

K

K

K

A

N

N

R

K

I

I

P

-

S

-

A

G

E

L

E

D

L

L

I

V

K

Q

E

E

A

A

-

A

-

V

E

A

S

Q

F

T

G

G

V

A

K

G

I

I

E

N

V

V

S

S

E

A

D

D

E

D

L

I

K

K

E

D

W

A

L

L

D

D

V

P

E

W

K

Q

S

N

L

V

E

L

R

R

K

E

N

G

V

K

G

G

G

M

T

P

A

A

K

P

E

M

E

S

L

V

E

K

R

A

M

S

I

I

R

D

E

D

R

A

E

M

R

A

K

E

I

L

S

H

E

K

D

D

E

R

E

A

K

W

L

L

K

A

V

N

L

A

K

T

S

Q

R

A

I

L

E

A

E

N

R

A

I

K

K

Q

H

T

L

L

M

A

N

D

E

S

V

V

E

Q

R

Q

V

I

C

I

binding (2~{S})-2-(2-azanylethylamino)butanedioic acid: S276 (= S268), S277 (= S269), M279 (= M271), K282 (= K274), N284 (= N276), D286 (= D278)
binding fumaric acid: R290 (≠ I282)

Sites not aligning to the query:

binding fumaric acid: 22

4adlA Crystal structures of rv1098c in complex with malate (see paper)
24% identity, 46% coverage: 72:292/481 of query aligns to 96:334/459 of 4adlA

query
sites
4adlA

S
|
S

G

G

E
x
T

D

S

V

S

H

N

E

M

A

N

I

T

E

N

S

E

K

V

V

I

V

A

E

S

I

I

A

A

K

A

E

K

S

G

G

G

M

V

K

T

M

L

H

H

T

P

-

N

-

D

-

V

-

N

-

M

A

S

R

Q

S
|
S

R
x
S

N
|
N

D

D

E

T

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

A

A

A

R

T

D

E

K

A

I

A

I

V

-

A

E

H

I

L

L

I

S

P

E

A

L

L

L

Q

E

Q

L

L

R

H

K

D

T

A

F

L

I

A

E

A

K

K

A

A

R

L

Q

D

Y

W

F

H

D

T

C

V

I

V

M

K

P

S

G

G

F

R

T
|
T

H
|
H

L

L

Q

M

Y

D

A

A

Q

V

P

P

T

V

K

T

L

L

S

G

H

Q

Y

E

L

F

I

S

A

G

Y

Y

H

A

D

R

A

Q

I

I

R

E

R

A

D

G

F

I

D

E

R

R

G

V

I

R

E

A

I

C

F

L

S

P

R

R

V

L

N

G

K

E

S

L

P

A

L

I

G

G

A

G

A

T

A

A

F

V

A

G

S

T

-

G

-

L

-

N

T

A

P

P

H

D

E

D

L

F

D

G

R

V

E

R

Y

V

V

V

A

A

Q

V

L

L

L

V

G

A

F

Q

D

T

G

G

L

L

V

S

E

E

-

L

-

R

-

T

-

A

N

N

T

S

M

F

D

E

A

A

S

Q

A

A

S

A

R

R

D

D

F

G

L

L

I

V

E

E

S

A

L

S

F

G

L

A

S

L

T

R

S

T

I

I

M

A

L

V

N

S

L

L

S

T

R

K

F

I

C

A

E

N

E

D

I

I

I

-

L

R

W

W

A

M

S

G

E

S

F

G

D

P

F

L

-

T

-

G

-

L

-

A

-

E

V

I

E

Q

L

L

P

P

D

D

E

-

F

L

S

Q

S

P

S

G

S
|
S

I

I

M

M

P

P

Q

G

K
|
K

K

V

N

N

P

P

D

V

V

L

A

P

E
|
E

I

A

I

V

R

T

A

Q

K

V

A

A

G

A

K

Q

S

V

V

I

G

G

active site: T178 (= T147), H179 (= H148), S310 (= S268), S311 (= S269), K316 (= K274), E323 (= E281)
binding (2S)-2-hydroxybutanedioic acid: S96 (= S72), T98 (≠ E74), S130 (= S100), S131 (≠ R101), N132 (= N102)

P9WN93 Fumarate hydratase class II; Fumarase C; Aerobic fumarase; Iron-independent fumarase; EC 4.2.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
24% identity, 46% coverage: 72:292/481 of query aligns to 104:342/474 of P9WN93

query
sites
P9WN93

S
|
S

G
|
G

E
x
T

D

S

V

S

H

N

E

M

A

N

I

T

E

N

S

E

K

V

V

I

V

A

E

S

I

I

A

A

K

A

E

K

S

G

G

G

M

V

K

T

M

L

H

H

T

P

-

N

-

D

-

V

-

N

-

M

A

S

R

Q

S
|
S

R
x
S

N
|
N

D

D

E

T

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

A

A

A

R

T

D

E

K

A

I

A

I

V

-

A

E

H

I

L

L

I

S

P

E

A

L

L

L

Q

E

Q

L

L

R

H

K

D

T

A

F

L

I

A

E

A

K

K

A

A

R

L

Q

D

Y

W

F

H

D

T

C

V

I

V

M

K

P

S

G

G

F

R

T
|
T

H

H

L

L

Q

M

Y

D

A

A

Q

V

P

P

T

V

K

T

L

L

S

G

H

Q

Y

E

L

F

I

S

A

G

Y

Y

H

A

D

R

A

Q

I

I

R

E

R

A

D

G

F

I

D

E

R

R

G

V

I

R

E

A

I

C

F

L

S

P

R

R

V

L

N

G

K

E

S

L

P

A

L

I

G

G

A

G

A

T

A

A

F

V

A

G

S

T

-

G

-

L

-

N

T

A

P

P

H

D

E

D

L

F

D

G

R

V

E

R

Y

V

V

V

A

A

Q

V

L

L

L

V

G

A

F

Q

D

T

G

G

L

L

V

S

E

E

-

L

-

R

-

T

-

A

N

N

T

S

M

F

D

E

A

A

S

Q

A

A

S

A

R

R

D

D

F

G

L

L

I

V

E

E

S

A

L

S

F

G

L

A

S

L

T

R

S

T

I

I

M

A

L

V

N

S

L

L

S

T

R

K

F

I

C

A

E

N

E

D

I

I

I

-

L

R

W

W

A

M

S

G

E

S

F

G

D

P

F

L

-

T

-

G

-

L

-

A

-

E

V

I

E

Q

L

L

P

P

D

D

E

-

F

L

S

Q

S

P

S

G

S
|
S

I

I

M

M

P

P

Q

G

K
|
K

K
x
V

N
|
N

P

P

D

V

V

L

A

P

E

E

I

A

I

V

R

T

A

Q

K

V

A

A

G

A

K

Q

S

V

V

I

G

G

SGT 104:106 (≠ SGE 72:74) binding substrate
SSN 138:140 (≠ SRN 100:102) binding substrate
T186 (= T147) binding substrate
S318 (= S268) active site; mutation S->A,C: Absence of fumarase activity.
S319 (= S269) binding substrate
KVN 324:326 (≠ KKN 274:276) binding substrate

4apbD Crystal structure of mycobacterium tuberculosis fumarase (rv1098c) s318c in complex with fumarate (see paper)
24% identity, 46% coverage: 72:292/481 of query aligns to 96:334/462 of 4apbD

query
sites
4apbD

S

S

G

G

E
x
T

D

S

V

S

H

N

E

M

A

N

I

T

E

N

S

E

K

V

V

I

V

A

E

S

I

I

A

A

K

A

E

K

S

G

G

G

M

V

K

T

M

L

H

H

T

P

-

N

-

D

-

V

-

N

-

M

A

S

R

Q

S
|
S

R
x
S

N
|
N

D

D

E

T

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

A

A

A

R

T

D

E

K

A

I

A

I

V

-

A

E

H

I

L

L

I

S

P

E

A

L

L

L

Q

E

Q

L

L

R

H

K

D

T

A

F

L

I

A

E

A

K

K

A

A

R

L

Q

D

Y

W

F

H

D

T

C

V

I

V

M

K

P

S

G

G

F

R

T
|
T

H
|
H

L

L

Q

M

Y

D

A

A

Q

V

P

P

T

V

K

T

L

L

S

G

H

Q

Y

E

L

F

I

S

A

G

Y

Y

H

A

D

R

A

Q

I

I

R

E

R

A

D

G

F

I

D

E

R

R

G

V

I

R

E

A

I

C

F

L

S

P

R

R

V

L

N

G

K

E

S

L

P

A

L

I

G

G

A

G

A

T

A

A

F

V

A

G

S

T

-

G

-

L

-

N

T

A

P

P

H

D

E

D

L

F

D

G

R

V

E

R

Y

V

V

V

A

A

Q

V

L

L

L

V

G

A

F

Q

D

T

G

G

L

L

V

S

E

E

-

L

-

R

-

T

-

A

N

N

T

S

M

F

D

E

A

A

S

Q

A

A

S

A

R

R

D

D

F

G

L

L

I

V

E

E

S

A

L

S

F

G

L

A

S

L

T

R

S

T

I

I

M

A

L

V

N

S

L

L

S

T

R

K

F

I

C

A

E

N

E

D

I

-

I

I

L

R

W

W

A

M

S

G

E

S

F

G

D

P

F

L

-

T

-

G

-

L

-

A

-

E

V

I

E

Q

L

L

P

P

D

D

E

-

F

L

S

Q

S

P

S

G

S
x
C

S
|
S

I

I

M
|
M

P

P

Q

G

K
|
K

K

V

N
|
N

P

P

D

V

V

L

A

P

E
|
E

I

A

I

V

R

T

A

Q

K

V

A

A

G

A

K

Q

S

V

V

I

G

G

active site: H179 (= H148), C310 (≠ S268), K316 (= K274), E323 (= E281)
binding fumaric acid: T98 (≠ E74), S130 (= S100), S131 (≠ R101), N132 (= N102), T178 (= T147), H179 (= H148), C310 (≠ S268), S311 (= S269), M313 (= M271), K316 (= K274), N318 (= N276)

Q9LCC6 Aspartate ammonia-lyase; Aspartase; EC 4.3.1.1 from Bacillus sp. (see 3 papers)
24% identity, 68% coverage: 33:361/481 of query aligns to 51:412/468 of Q9LCC6

query
sites
Q9LCC6

L

I

V

V

D

K

Y

K

A

S

H

A

V

A

L

L

G

A

L

N

L

M

K

E

V

V

G

G

L

L

I

L

T

D

K

K

E

E

D

V

A

G

K

Q

R

Y

I

I

F

V

E

K

A

A

L

A

R

D

E

E

V

V

-

I

-

E

-

G

-

K

-

W

-

N

E

D

K

Q

L

F

G

I

L

V

E

D

R

P

L

I

S

Q

G

G

E

G

-

A

-

G

-
x
T

-

S

-

I

-

N

-

M

D

N

V

A

H

N

E

E

A

V

I

I

E

A

S

N

K

R

V

A

V

L

E

E

I

L

A

M

K

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

S

P

G

N

M

S

K
x
H

M

V

H

N

T

M

A

S

R

Q

S
|
S

R
x
T

N
|
N

D

D

E

A

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

-

A

-

R

-

D

-

K

A

I

V

I

L

E

S

I

L

L

L

S

N

E

Q

L

L

L

I

E

E

-

T

-

K

-

Y

L

M

R

Q

K

Q

T

E

F

F

I

M

E

K

K

K

A

A

R

D

Q

E

Y

F

F

A

D

G

C

V

I

I

M
x
K

P

M

G

G

F

R

T
|
T

H
|
H

L

L

Q

Q

Y

D

A

A

Q

V

P

P

T

I

K

L

L

L

S

G

H

Q

Y

E

L

F

I

E

A

A

Y

Y

H

A

D

R

A

V

I

I

R

A

R

R

D

D

F

I

D

E

R

R

G

I

I

A

E

N

I

T

F

R

S

N

R

N

V

L

N

Y

K

D

S

I

P

N

L

M

G

G

A

A

A

T

A

A

F

V

A

G

S

-

T

T

P

G

H

L

E

N

L

A

D

D

R

P

E

E

Y

Y

V

I

A

S

-

I

-

V

-

T

-

E

Q

H

L

L

L

A

G

K

F

F

D

S

G

G

-

H

-

P

-

L

-

R

L

S

V

A

E

Q

N

H

T

L

M

V

D

D

A

A

S

T

A

Q

S

N

R

T

D

D

F

C

L

Y

I

T

E

E

S

V

L

S

F

S

L

A

S

L

T

K

S

V

I

C

M

M

L

I

N

N

L

M

S

S

R

K

F

I

C

A

E

N

E

D

I

L

I

R

L

L

W

M

A

A

S

S

-

G

-

P

-

R

-

A

E

G

F

L

D

S

F

E

V

I

E

V

L

L

P

P

D

A

E

R

F

-

S

Q

S

P

S

G

S
|
S

I
|
I

M
|
M

P
|
P

Q

G

K
|
K

K

V

N
|
N

P

P

D

V

V

M

A

P

E

E

I

V

I

M

R

N

A

Q

K

V

A

A

G

F

K

Q

S

V

V

F

G

G

Y

-

L

-

A

-

S

N

A

D

C

L

T

T

I

I

L

T

K

S

A

A

L

-

P

S

Y

E

A

A

Y

G

N

Q

R

F

D

E

F

L

Q

N

E

V

M

M

N

E

S

P

L

V

L

L

Y

F

V

F

S

N

M

L

N

I

E

Q

C

S

L

I

L

S

S

I

V

M

K

T

T

N

F

V

R

F

K

K

M

S

F

F

S

T

-

E

-

N

-

C

-

L

-

K

A

G

L

I

K

K

F

A

K

N

E

E

V

R

M

M

R

K

E

E

K

Y

A

V

S

E

K

K

G

S

L

I

T

G

L

I

A

I

T

T

D

A

L

I

T101 (vs. gap) binding L-aspartate; mutation to A: 7100-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to S: 80-fold decrease in catalytic efficiency.
H134 (≠ K94) mutation to A: Retains full activity. Shows a slightly stronger affinity for L-aspartate. Does not affect tertiary structure.
S140 (= S100) binding L-aspartate; mutation to A: 27-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation S->K,R: Loss of activity.
T141 (≠ R101) binding L-aspartate; mutation to A: 15-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to K: 40000-fold decrease in catalytic efficiency.; mutation T->V,R: Loss of activity.
N142 (= N102) binding L-aspartate; mutation to A: Loss of activity. Does not result in any major conformational changes.; mutation to Q: 3000-fold decrease in catalytic efficiency.
K183 (≠ M143) mutation to A: Loss of activity. Does not affect tertiary structure.
T187 (= T147) binding L-aspartate; mutation to A: 6280-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to S: 2.3-fold decrease in catalytic efficiency.
H188 (= H148) binding L-aspartate; mutation to A: 100-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation H->K,Q,R: Loss of activity.
S318 (= S268) mutation to A: Loss of activity.
S319 (= S269) binding L-aspartate; mutation to A: Almost no change in catalytic efficiency.
I320 (= I270) mutation to A: 50-fold decrease in catalytic efficiency.
M321 (= M271) mutation to A: 338-fold decrease in catalytic efficiency.
P322 (= P272) mutation to A: Almost no change in catalytic efficiency.
K324 (= K274) binding L-aspartate; mutation to A: Loss of activity. Does not result in any major conformational changes.; mutation K->D,H,R,S,V: Loss of activity.
N326 (= N276) mutation to A: 22500-fold decrease in catalytic efficiency. Does not result in any major conformational changes.; mutation to Q: 168750-fold decrease in catalytic efficiency.

3r6qA A triclinic-lattice structure of aspartase from bacillus sp. Ym55-1 (see paper)
24% identity, 68% coverage: 33:361/481 of query aligns to 47:408/462 of 3r6qA

query
sites
3r6qA

L

I

V

V

D

K

Y

K

A

S

H

A

V

A

L

L

G

A

L

N

L

M

K

E

V

V

G

G

L

L

I

L

T

D

K

K

E

E

D

V

A

G

K

Q

R

Y

I

I

F

V

E

K

A

A

L

A

R

D

E

E

V

V

-

I

-

E

-

G

-

K

-

W

-

N

E

D

K

Q

L

F

G

I

L

V

E

D

R

P

L

I

S

Q

G

G

E

G

-

A

-

G

-

T

-

S

-

I

-

N

-

M

D

N

V

A

H

N

E

E

A

V

I

I

E

A

S

N

K

R

V

A

V

L

E

E

I

L

A

M

K

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

S

P

G

N

M

S

K

H

M

V

H

N

T

M

A

S

R

Q

S

S

R

T

N

N

D

D

E

A

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

-

A

-

R

-

D

-

K

A

I

V

I

L

E

S

I

L

L

L

S

N

E

Q

L

L

L

I

E

E

-

T

-

K

-

Y

L

M

R

Q

K

Q

T

E

F

F

I

M

E

K

K

K

A

A

R

D

Q

E

Y

F

F

A

D

G

C

V

I

I

M

K

P

M

G

G

F

R

T

T

H
|
H

L

L

Q

Q

Y

D

A

A

Q

V

P

P

T

I

K

L

L

L

S

G

H

Q

Y

E

L

F

I

E

A

A

Y

Y

H

A

D

R

A

V

I

I

R

A

R

R

D

D

F

I

D

E

R

R

G

I

I

A

E

N

I

T

F

R

S

N

R

N

V

L

N

Y

K

D

S

I

P

N

L

M

G

G

A

A

A

T

A

A

F

V

A

G

S

-

T

T

P

G

H

L

E

N

L

A

D

D

R

P

E

E

Y

Y

V

I

A

S

-

I

-

V

-

T

-

E

Q

H

L

L

L

A

G

K

F

F

D

S

G

G

-

H

-

P

-

L

-

R

L

S

V

A

E

Q

N

H

T

L

M

V

D

D

A

A

S

T

A

Q

S

N

R

T

D

D

F

C

L

Y

I

T

E

E

S

V

L

S

F

S

L

A

S

L

T

K

S

V

I

C

M

M

L

I

N

N

L

M

S

S

R

K

F

I

C

A

E

N

E

D

I

L

I

R

L

L

W

M

A

A

S

S

-

G

-

P

-

R

-

A

E

G

F

L

D

S

F

E

V

I

E

V

L

L

P

P

D

A

E

R

F

-

S

Q

S

P

S

G

S
|
S

S

S

I

I

M

M

P

P

Q

G

K
|
K

K

V

N

N

P

P

D

V

V

M

A

P

E
|
E

I

V

I

M

R

N

A

Q

K

V

A

A

G

F

K

Q

S

V

V

F

G

G

Y

-

L

-

A

-

S

N

A

D

C

L

T

T

I

I

L

T

K

S

A

A

L

-

P

S

Y

E

A

A

Y

G

N

Q

R

F

D

E

F

L

Q

N

E

V

M

M

N

E

S

P

L

V

L

L

Y

F

V

F

S

N

M

L

N

I

E

Q

C

S

L

I

L

S

S

I

V

M

K

T

T

N

F

V

R

F

K

K

M

S

F

F

S

T

-

E

-

N

-

C

-

L

-

K

A

G

L

I

K

K

F
x
A

K

N

E
|
E

E

E

V

R

M

M

R

K

E

E

K

Y

A

V

S

E

K

K

G

S

L

I

T

G

L

I

A

I

T

T

D

A

L

I

active site: H184 (= H148), S314 (= S268), K320 (= K274), E327 (= E281)
binding calcium ion: A389 (≠ F342), E391 (= E344)

3r6vG Crystal structure of aspartase from bacillus sp. Ym55-1 with bound l- aspartate (see paper)
24% identity, 68% coverage: 33:361/481 of query aligns to 48:409/463 of 3r6vG

query
sites
3r6vG

L

I

V

V

D

K

Y

K

A

S

H

A

V

A

L

L

G

A

L

N

L

M

K

E

V

V

G

G

L

L

I

L

T

D

K

K

E

E

D

V

A

G

K

Q

R

Y

I

I

F

V

E

K

A

A

L

A

R

D

E

E

V

V

-

I

-

E

-

G

-

K

-

W

-

N

E

D

K

Q

L

F

G

I

L

V

E

D

R

P

L

I

S

Q

G

G

E

G

-

A

-

G

-

T

-

S

-

I

-

N

-

M

D

N

V

A

H

N

E

E

A

V

I

I

E

A

S

N

K

R

V

A

V

L

E

E

I

L

A

M

K

G

E

E

-

E

-

K

-

G

-

N

-

Y

-

S

-

K

-

I

-

S

S

P

G

N

M

S

K

H

M

V

H

N

T

M

A

S

R

Q

S

S

R

T

N

N

D

D

E

A

I

F

A

P

A

T

C

A

L

T

R

H

L

I

F

-

A

-

R

-

D

-

K

A

I

V

I

L

E

S

I

L

L

L

S

N

E

Q

L

L

L

I

E

E

-

T

-

K

-

Y

L

M

R

Q

K

Q

T

E

F

F

I

M

E

K

K

K

A

A

R

D

Q

E

Y

F

F

A

D

G

C

V

I

I

M

K

P

M

G

G

F

R

T

T

H
|
H

L

L

Q

Q

Y

D

A

A

Q

V

P

P

T

I

K

L

L

L

S

G

H

Q

Y

E

L

F

I

E

A

A

Y

Y

H

A

D

R

A

V

I

I

R

A

R

R

D

D

F

I

D

E

R

R

G

I

I

A

E

N

I

T

F

R

S

N

R

N

V

L

N

Y

K

D

S

I

P

N

L

M

G

G

A

A

A

T

A

A

F

V

A

G

S

-

T

T

P

G

H

L

E

N

L

A

D

D

R

P

E

E

Y

Y

V

I

A

S

-

I

-

V

-

T

-

E

Q

H

L

L

L

A

G

K

F

F

D

S

G

G

-

H

-

P

-

L

-

R

L

S

V

A

E

Q

N

H

T

L

M

V

D

D

A

A

S

T

A

Q

S

N

R

T

D

D

F

C

L

Y

I

T

E

E

S

V

L

S

F

S

L

A

S

L

T

K

S

V

I

C

M

M

L

I

N

N

L

M

S

S

R

K

F

I

C

A

E

N

E

D

I

L

I

R

L

L

W

M

A

A

S

S

-

G

-

P

-

R

-

A

E

G

F

L

D

S

F

E

V

I

E

V

L

L

P

P

D

A

E

R

F

-

S

Q

S

P

S
x
G

S
|
S

I

I

M

M

P

P

Q

G

K
|
K

K

V

N

N

P

P

D

V

V

M

A

P

E
|
E

I

V

I

M

R

N

A

Q

K

V

A

A

G

F

K

Q

S

V

V

F

G

G

Y

-

L

-

A

-

S

N

A

D

C

L

T

T

I

I

L

T

K

S

A

A

L

-

P

S

Y

E

A

A

Y

G

N

Q

R

F

D

E

F

L

Q

N

E

V

M

M

N

E

S

P

L

V

L

L

Y

F

V

F

S

N

M

L

N

I

E

Q

C

S

L

I

L

S

S

I

V

M

K

T

T

N

F

V

R

F

K

K

M

S

F

F

S

T

-

E

-

N

-

C

-

L

-

K

A

G

L

I

K

K

F

A

K

N

E

E

V

R

M

M

R

K

E

E

K

Y

A

V

S

E

K

K

G

S

L

I

T

G

L

I

A

I

T

T

D

A

L

I

active site: H185 (= H148), S315 (= S268), K321 (= K274), E328 (= E281)
binding aspartic acid: G314 (≠ S267), S315 (= S268), S316 (= S269)

Query Sequence

>WP_012965390.1 NCBI__GCF_000025505.1:WP_012965390.1
MIRSRLSGSLDEDALKLTSSLEHDRHIFKYDILVDYAHVLGLLKVGLITKEDAKRIFEAL
REVEKLGLERLSGEDVHEAIESKVVEIAKESGMKMHTARSRNDEIAACLRLFARDKIIEI
LSELLELRKTFIEKARQYFDCIMPGFTHLQYAQPTKLSHYLIAYHDAIRRDFDRGIEIFS
RVNKSPLGAAAFASTPHELDREYVAQLLGFDGLVENTMDASASRDFLIESLFLSTSIMLN
LSRFCEEIILWASEFDFVELPDEFSSSSSIMPQKKNPDVAEIIRAKAGKSVGYLASACTI
LKALPYAYNRDFQEMNSLLYVSMNECLLSVKTFRKMFSALKFKEEVMREKASKGLTLATD
LADLLVRKGVPFRLSHKIVGSLVKKNRIPPSAEELIKEAESFGVKIEVSEDELKEWLDVE
KSLERRKNVGGTAKEELERMIRERERKISEDEEKLKVLKSRIEERIKHLMNEVERVCSGK
G

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory