SitesBLAST

O30207 O-phospho-L-seryl-tRNA:Cys-tRNA synthase 1; Sep-tRNA:Cys-tRNA synthase 1; SepCysS 1; EC 2.5.1.73 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
47% identity, 96% coverage: 11:388/394 of query aligns to 2:370/371 of O30207

query
sites
O30207

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AR 78:79 (= AR 86:87) binding pyridoxal 5'-phosphate
N183 (= N191) binding pyridoxal 5'-phosphate
SGH 206:208 (= SGH 214:216) binding pyridoxal 5'-phosphate
K209 (= K217) modified: N6-(pyridoxal phosphate)lysine

2e7iA Crystal structure of sep-trna:cys-tRNA synthase from archaeoglobus fulgidus (see paper)
45% identity, 94% coverage: 19:388/394 of query aligns to 3:343/344 of 2e7iA

query
sites
2e7iA

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binding pyridoxal-5'-phosphate: G50 (= G85), A51 (= A86), R52 (= R87), H76 (= H109), N156 (= N191), A158 (= A193), Y159 (= Y194), S179 (= S214), H181 (= H216), K182 (= K217)
binding sulfate ion: R52 (= R87), Y77 (= Y110), R240 (= R284), R243 (≠ K287)

5b7uA Apo structure of cysteine desulfurase from thermococcus onnurineus na1 at 1.89a (see paper)
38% identity, 20% coverage: 158:236/394 of query aligns to 162:240/402 of 5b7uA

query
sites
5b7uA

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active site: D195 (≠ N191), A197 (= A193), Q198 (≠ Y194), K221 (= K217)

Sites not aligning to the query:

active site: 119, 356, 361
binding cysteine: 45, 275

5b87A Crystal structure of a cysteine desulfurase from thermococcus onnurineus na1 in complex with alanine at 2.3 angstrom resolution (see paper)
38% identity, 20% coverage: 158:236/394 of query aligns to 156:234/397 of 5b87A

query
sites
5b87A

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active site: D189 (≠ N191), A191 (= A193), Q192 (≠ Y194), K215 (= K217)
binding alanine: N164 (≠ Y166), K215 (= K217)
binding pyridoxal-5'-phosphate: N164 (≠ Y166), D189 (≠ N191), A191 (= A193), Q192 (≠ Y194), S212 (= S214), H214 (= H216), K215 (= K217)

Sites not aligning to the query:

active site: 113, 350, 355
binding alanine: 23, 370
binding pyridoxal-5'-phosphate: 84, 85, 86, 113

Q8KUU5 Cysteine desulfurase; CyD; EC 2.8.1.7 from Synechococcus elongatus (strain ATCC 33912 / PCC 7942 / FACHB-805) (Anacystis nidulans R2) (see paper)
24% identity, 43% coverage: 68:236/394 of query aligns to 291:462/626 of Q8KUU5

query
sites
Q8KUU5

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Sites not aligning to the query:

1:225 Cargo-loading domain; mutation Missing: No longer loaded into nanocompartments, no change in catalytic efficiency.

7xekA Sufs with d-cysteine for 6 h
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/413 of 7xekA

query
sites
7xekA

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L

binding (2~{S},4~{S})-2-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]-1,3-thiazolidine-4-carboxylic acid: N173 (≠ Y166), D198 (≠ N191), A200 (= A193), S221 (= S214), H223 (= H216), K224 (= K217)

Sites not aligning to the query:

binding (2~{S},4~{S})-2-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]-1,3-thiazolidine-4-carboxylic acid: 28, 29, 92, 93, 121, 376

7xejA Sufs with d-cysteine for 5 min
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/413 of 7xejA

query
sites
7xejA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding (2~{S})-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-propanoic acid: N173 (≠ Y166), D198 (≠ N191), A200 (= A193), S221 (= S214), H223 (= H216), K224 (= K217)

Sites not aligning to the query:

binding (2~{S})-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-propanoic acid: 28, 29, 92, 93, 121, 376

7xetA Sufs with beta-cyano-l-alanine
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/406 of 7xetA

query
sites
7xetA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding (2~{E})-3-cyano-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylimino]propanoic acid: N173 (≠ Y166), D198 (≠ N191), A200 (= A193), S221 (= S214), H223 (= H216), K224 (= K217)

Sites not aligning to the query:

binding (2~{E})-3-cyano-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylimino]propanoic acid: 28, 29, 93, 121, 356, 376

O32164 Cysteine desulfurase SufS; EC 2.8.1.7 from Bacillus subtilis (strain 168) (see 3 papers)
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/406 of O32164

query
sites
O32164

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y

N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N

D

T

G

A

A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S

S

G

S

H

H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

K224 (= K217) modified: N6-(pyridoxal phosphate)lysine

Sites not aligning to the query:

361 active site, Cysteine persulfide intermediate; C→A: Loss of cysteine desulfurase activity, still binds SufU and Cys.

7cepA Crystal structure of l-cycloserine-bound form of cysteine desulfurase sufs from bacillus subtilis (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 153:236/414 of 7cepA

query
sites
7cepA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: N175 (≠ Y166), D200 (≠ N191), A202 (= A193), S223 (= S214), H225 (= H216), K226 (= K217)

Sites not aligning to the query:

binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: 30, 31, 94, 95, 123, 378

7ceoA Crystal structure of pmp-bound form of cysteine desulfurase sufs from bacillus subtilis (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/412 of 7ceoA

query
sites
7ceoA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y

N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: D198 (≠ N191), A200 (= A193), S221 (= S214), H223 (= H216), K224 (= K217)

Sites not aligning to the query:

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: 92, 93, 121

5zsoA Sufs from bacillus subtilis, soaked with l-cysteine for 90 sec (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/405 of 5zsoA

query
sites
5zsoA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H

H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: N173 (≠ Y166), D198 (≠ N191), A200 (= A193), S221 (= S214), K224 (= K217)

Sites not aligning to the query:

binding n-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-cysteine: 28, 29, 92, 93, 121, 356, 361, 376

7cerA Crystal structure of d-cycloserine-bound form of cysteine desulfurase sufs h121a from bacillus subtilis (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 153:236/414 of 7cerA

query
sites
7cerA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: N175 (≠ Y166), D200 (≠ N191), A202 (= A193), S223 (= S214), H225 (= H216), K226 (= K217)

Sites not aligning to the query:

binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: 30, 94, 95, 358, 378

5xt6A A sulfur-transferring catalytic intermediate of sufs-sufu complex from bacillus subtilis (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 152:235/405 of 5xt6A

query
sites
5xt6A

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y
x
Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: N174 (≠ Y166), D199 (≠ N191), A201 (= A193), Q202 (≠ Y194), S222 (= S214), H224 (= H216), K225 (= K217)

Sites not aligning to the query:

binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: 55, 93, 94, 122, 275, 276, 357, 377

7xenA Sufs with l-penicillamine
28% identity, 22% coverage: 144:228/394 of query aligns to 138:221/392 of 7xenA

query
sites
7xenA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y
x
Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H

H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding (2~{R})-3-methyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-butanoic acid: N160 (≠ Y166), D185 (≠ N191), A187 (= A193), Q188 (≠ Y194), S208 (= S214), K211 (= K217)

Sites not aligning to the query:

binding (2~{R})-3-methyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]-3-sulfanyl-butanoic acid: 28, 29, 79, 80, 108, 347, 363

7xepA Sufs with l-propargylglycine
28% identity, 22% coverage: 144:228/394 of query aligns to 153:236/408 of 7xepA

query
sites
7xepA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y
x
N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y

Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

binding [6-methyl-4-[[(5-methyl-2-oxidanylidene-furan-3-ylidene)amino]methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: N175 (≠ Y166), D200 (≠ N191), A202 (= A193), S223 (= S214), H225 (= H216), K226 (= K217)

Sites not aligning to the query:

binding [6-methyl-4-[[(5-methyl-2-oxidanylidene-furan-3-ylidene)amino]methyl]-5-oxidanyl-pyridin-3-yl]methyl dihydrogen phosphate: 30, 31, 94, 95, 123, 378

5j8qA Crystal structure of the cysteine desulfurase sufs of bacillus subtilis (see paper)
28% identity, 22% coverage: 144:228/394 of query aligns to 151:234/413 of 5j8qA

query
sites
5j8qA

T

S

F

L

E

E

R

D

V

V

K

R

E

E

E

T

T

V

G

T

K

S

Y

N

P

T

D

K

L

I

A

V

L

A

L

V

T

S

H

H

V

V

D

S

Y

N

R

V

Y

L

G

G

N

T

L

V

A

N

D

P

A

I

K

K

E

E

V

M

G

A

K

K

I

I

C

A

R

H

E

D

Y

N

G

G

I

A

P

V

L

I

I

V

L

V

N
x
D

T

G

A
|
A

Y
x
Q

S

S

S

T

G

P

V

H

M

M

E

K

V

I

D

D

G

V

K

Q

K

D

L

L

N

D

A

C

D

D

F

F

I

F

V

A

A

L

S
|
S

G

S

H
|
H

K
|
K

S

M

W

C

A

G

A

P

T

T

A

G

P

-

I

V

G

G

I

V

L

L

active site: D198 (≠ N191), A200 (= A193), Q201 (≠ Y194), K224 (= K217)
binding pyridoxal-5'-phosphate: D198 (≠ N191), A200 (= A193), S221 (= S214), H223 (= H216), K224 (= K217)

Sites not aligning to the query:

active site: 121, 356, 361
binding alanine: 28, 29, 121, 360, 376
binding pyridoxal-5'-phosphate: 92, 93, 121

Query Sequence

>WP_012966499.1 NCBI__GCF_000025505.1:WP_012966499.1
MNLDRYSPQNLRRPSSGMINVHPIQRGGILTEEARQVILEWADGYSVCDLCLEGRVDLVK
NPPIAEFKRDVAEFLNMDEVRFTAGARHAKFVIMSAFKEKTIVVDSLAHYTSYIAAELND
MKVYEVPNSGYPEFKIEPEKYAETFERVKEETGKYPDLALLTHVDYRYGNLADAKEVGKI
CREYGIPLILNTAYSSGVMEVDGKKLNADFIVASGHKSWAATAPIGILATNYDFVDDVFR
TSKTRGEWSGRAFTKKEVALFGCSPVYGLPIITLMASFPAVVRRIKKWEEEVAKARWFVK
EMEKIEGVQLIGERPKNHTLMQFESPAFHSIAKRHKKRGFFLYHELKKRGIVGVQPGMTK
NFKINVYGLSWEEVEKVAEAFKDIAESYGLEVED

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory