SitesBLAST
Comparing WP_012966505.1 NCBI__GCF_000025505.1:WP_012966505.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
46% identity, 99% coverage: 3:253/253 of query aligns to 4:267/268 of 4ywjA
- active site: H156 (= H146), K160 (= K150)
- binding nicotinamide-adenine-dinucleotide: G11 (= G10), R12 (= R11), M13 (= M12), D35 (≠ Q29), R36 (≠ A30), F76 (= F67), T77 (≠ S68), V81 (≠ A72), G99 (= G90), T101 (= T92), A124 (≠ P115), N125 (= N116), F126 (= F117), R237 (= R222), F240 (= F225)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
45% identity, 99% coverage: 3:253/253 of query aligns to 4:266/266 of 5temA
- active site: H155 (= H146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), R12 (= R11), M13 (= M12), E34 (≠ V33), R35 (= R34), F75 (= F67), T76 (≠ S68), S80 (≠ A72), G98 (= G90), T100 (= T92), P123 (= P115), N124 (= N116), Y125 (≠ F117), F239 (= F225)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T92), P123 (= P115), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
45% identity, 99% coverage: 3:253/253 of query aligns to 4:266/269 of 5tejB
- active site: H155 (= H146), K159 (= K150)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T92), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), R12 (= R11), M13 (= M12), E34 (≠ V33), R35 (= R34), F75 (= F67), T76 (≠ S68), S80 (≠ A72), G98 (= G90), T100 (= T92), P123 (= P115), N124 (= N116), Y125 (≠ F117), F239 (= F225)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
45% identity, 99% coverage: 3:253/253 of query aligns to 4:266/269 of 5tejA
- active site: H155 (= H146), K159 (= K150)
- binding nicotinamide-adenine-dinucleotide: G8 (= G7), G11 (= G10), R12 (= R11), M13 (= M12), E34 (≠ V33), R35 (= R34), F75 (= F67), T76 (≠ S68), S80 (≠ A72), G98 (= G90), T100 (= T92), P123 (= P115)
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 2:264/269 of 1arzB
- active site: H155 (= H146), K159 (= K150)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G7), G10 (≠ A9), G11 (= G10), R12 (= R11), M13 (= M12), E34 (≠ V33), F75 (= F67), T76 (≠ S68), R77 (≠ V69), G80 (≠ A72), H84 (≠ L76), G98 (= G90), T100 (= T92), A123 (≠ P115), N124 (= N116), F125 (= F117), F239 (= F225)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T92), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157), F239 (= F225)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
44% identity, 99% coverage: 1:251/253 of query aligns to 6:268/273 of P04036
- G12 (= G7) binding
- GRM 15:17 (= GRM 10:12) binding
- RM 16:17 (= RM 11:12) binding
- E38 (≠ V33) binding
- R39 (= R34) binding
- TR 80:81 (≠ SV 68:69) binding
- GTT 102:104 (= GTT 90:92) binding ; binding
- AANF 126:129 (≠ SPNF 114:117) binding
- F129 (= F117) binding
- H159 (= H146) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K150) binding ; mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R222) binding
- F243 (= F225) binding
1drvA Escherichia coli dhpr/acnadh complex (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 3:265/270 of 1drvA
- active site: H156 (= H146), K160 (= K150)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G7), G12 (= G10), R13 (= R11), M14 (= M12), E35 (≠ V33), F76 (= F67), T77 (≠ S68), R78 (≠ V69), G81 (≠ A72), G99 (= G90), A124 (≠ P115), F126 (= F117), R237 (= R222)
1druA Escherichia coli dhpr/nadh complex (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 3:265/270 of 1druA
- active site: H156 (= H146), K160 (= K150)
- binding nicotinamide-adenine-dinucleotide: G9 (= G7), G12 (= G10), R13 (= R11), M14 (= M12), E35 (≠ V33), R36 (= R34), F76 (= F67), T77 (≠ S68), R78 (≠ V69), G81 (≠ A72), G99 (= G90), T100 (= T91), T101 (= T92), A124 (≠ P115), N125 (= N116), F126 (= F117), F240 (= F225)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 3:265/270 of 1arzA
1drwA Escherichia coli dhpr/nhdh complex (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 5:267/272 of 1drwA
- active site: H158 (= H146), K162 (= K150)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G7), G14 (= G10), R15 (= R11), M16 (= M12), E37 (≠ V33), R38 (= R34), F78 (= F67), T79 (≠ S68), R80 (≠ V69), G101 (= G90), T102 (= T91), T103 (= T92), A126 (≠ P115), N127 (= N116), F128 (= F117), F242 (= F225)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
44% identity, 99% coverage: 1:251/253 of query aligns to 5:267/272 of 1dihA
- active site: H158 (= H146), K162 (= K150)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G7), G14 (= G10), R15 (= R11), M16 (= M12), R38 (= R34), F78 (= F67), T79 (≠ S68), R80 (≠ V69), G83 (≠ A72), G101 (= G90), T103 (= T92), N127 (= N116), F128 (= F117), R239 (= R222), F242 (= F225)
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
38% identity, 99% coverage: 1:251/253 of query aligns to 1:212/216 of Q9X1K8
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
38% identity, 99% coverage: 1:251/253 of query aligns to 6:217/218 of 1vm6B
- active site: H132 (= H146), K136 (= K150)
- binding nicotinamide-adenine-dinucleotide: G12 (= G7), S14 (≠ A9), G15 (= G10), R16 (= R11), M17 (= M12), D37 (= D32), V38 (= V33), F53 (= F67), S54 (= S68), S55 (≠ V69), E57 (= E71), A58 (= A72), G76 (= G90), T78 (= T92), Y101 (≠ P115), N102 (= N116), F103 (= F117), F192 (= F225)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
39% identity, 100% coverage: 1:253/253 of query aligns to 2:266/267 of 3ijpB
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
39% identity, 100% coverage: 1:253/253 of query aligns to 2:266/266 of 3ijpA
- active site: H155 (= H146), K159 (= K150)
- binding sodium ion: I21 (≠ V21), Q22 (≠ E22), R24 (≠ N24), V27 (= V27)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G7), N10 (≠ A9), G11 (= G10), R12 (= R11), M13 (= M12), R35 (= R34), F75 (= F67), S76 (= S68), Q77 (≠ V69), A80 (= A72), G98 (= G90), T100 (= T92), G123 (≠ P115), N124 (= N116), M125 (≠ F117), F239 (= F225)
5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
32% identity, 99% coverage: 1:251/253 of query aligns to 2:230/230 of 5wolA
- active site: H133 (= H146), K137 (= K150)
- binding pyridine-2-carboxylic acid: P104 (= P115), T144 (= T157), K147 (= K160)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (= N6), G11 (= G10), K12 (≠ R11), M13 (= M12), G34 (= G47), R35 (≠ K48), F54 (= F67), T55 (≠ S68), T56 (≠ V69), S59 (≠ A72), G77 (= G90), T78 (= T91), T79 (= T92), P104 (= P115), N105 (= N116), F106 (= F117)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
28% identity, 98% coverage: 3:251/253 of query aligns to 5:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ A9), G12 (= G10), K13 (≠ R11), M14 (= M12), D35 (= D32), H36 (≠ V33), K37 (≠ R34), L76 (≠ F67), T77 (≠ S68), G99 (= G90), T100 (= T91), T101 (= T92), P126 (= P115), N127 (= N116), F128 (= F117)
- binding pyridine-2,6-dicarboxylic acid: P126 (= P115), H155 (= H146), H156 (= H147), K159 (= K150), S164 (= S155), G165 (= G156), T166 (= T157), A215 (≠ G200)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
28% identity, 98% coverage: 3:251/253 of query aligns to 5:265/265 of 5z2eA
1p9lA Structure of m. Tuberculosis dihydrodipicolinate reductase in complex with nadh and 2,6 pdc (see paper)
28% identity, 96% coverage: 1:244/253 of query aligns to 1:234/245 of 1p9lA
- active site: H132 (= H146), K136 (= K150)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G7 (= G7), G10 (= G10), K11 (≠ R11), V12 (≠ M12), D33 (= D39), A34 (= A40), F52 (= F67), T53 (≠ S68), V57 (≠ A72), G75 (= G90), T77 (= T92), P103 (= P115), N104 (= N116), F105 (= F117), F217 (= F225)
- binding pyridine-2,6-dicarboxylic acid: H133 (= H147), K136 (= K150), S141 (= S155), G142 (= G156), T143 (= T157), A192 (≠ G200)
1c3vA Dihydrodipicolinate reductase from mycobacterium tuberculosis complexed with NADPH and pdc (see paper)
28% identity, 96% coverage: 1:244/253 of query aligns to 1:234/245 of 1c3vA
- active site: H132 (= H146), K136 (= K150)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: K9 (≠ A9), G10 (= G10), K11 (≠ R11), V12 (≠ M12), D33 (= D39), A34 (= A40), F52 (= F67), T53 (≠ S68), V57 (≠ A72), G75 (= G90), T77 (= T92), P103 (= P115), N104 (= N116), F217 (= F225)
- binding pyridine-2,6-dicarboxylic acid: T77 (= T92), N104 (= N116), K136 (= K150), S141 (= S155), G142 (= G156), T143 (= T157), A192 (≠ G200)
Query Sequence
>WP_012966505.1 NCBI__GCF_000025505.1:WP_012966505.1
MNVCVNGAAGRMGRLVVKNAVEANHKVVQAFDVREIGKDAGELAGVGKIGTLIQEMSDLN
CDVAIDFSVPEATIKLVELALKSNTKLVIGTTGFNDEQKRVIEEASKKIPIVMSPNFSVG
VNVFWKIIEEASKLLRDYDVEILEIHHRFKRDAPSGTALKAAEIVKNEIGVEKLVFGRSG
ECLRGDEIGVMAIRGGDVVGEHYVMFIGFGERIEINHKAWSREIFARGAIKAAEWVMNVD
KPGLYGMRDVLGV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory