SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012967145.1 NCBI__GCF_000025465.1:WP_012967145.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
87% identity, 100% coverage: 1:404/406 of query aligns to 1:404/405 of P40732

query
sites
P40732

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GT 108:109 (= GT 108:109) binding pyridoxal 5'-phosphate
K255 (= K255) modified: N6-(pyridoxal phosphate)lysine
T284 (= T284) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
86% identity, 98% coverage: 7:404/406 of query aligns to 2:399/402 of 4jevB

query
sites
4jevB

A

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active site: F136 (= F141), E188 (= E193), D221 (= D226), Q224 (= Q229), K250 (= K255), T279 (= T284), R372 (= R377)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (= I51), S102 (= S107), G103 (= G108), T104 (= T109), F136 (= F141), H137 (= H142), E188 (= E193), E193 (= E198), D221 (= D226), V223 (= V228), Q224 (= Q229), K250 (= K255), R372 (= R377)

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
85% identity, 98% coverage: 7:404/406 of query aligns to 2:394/397 of 4jewA

query
sites
4jewA

A

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active site: F136 (= F141), E188 (= E193), D221 (= D226), Q224 (= Q229), K250 (= K255), T274 (= T284), R367 (= R377)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: G103 (= G108), T104 (= T109), F136 (= F141), H137 (= H142), R139 (= R144), E188 (= E193), E193 (= E198), D221 (= D226), V223 (= V228), K250 (= K255)
binding picric acid: K25 (= K30), K27 (= K32), W32 (= W37)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
85% identity, 97% coverage: 12:404/406 of query aligns to 1:388/389 of 2pb0A

query
sites
2pb0A

T

T

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H

K

E

S

Q

Q

G

G

E

E

T

T

L

L

W

W

H

H

T

T

S

S

N

N

V

V

F

F

T

T

N

N

E

E

P

P

A

A

L

L

R

R

L

L

G

G

C

R

K

K

L

L

V

I

E

D

A

A

T

T

F

F

A

A

E

E

R

R

V

V

V

L

F

F

M

M

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

T

A

A

F

F

K

K

L

L

A

A

R

R

H

H

Y

Y

A

A

V

C

T

V

R

R

H

H

S

S

P

P

Y

F

K

K

T

T

K

K

I

I

A

A

F

F

H

H

N

N

A

A

F
|
F

H
|
H

G

G

R

R

S

S

L

L

F

F

T

T

V

V

S

S

V

V

G

G

Q

Q

P

P

K

K

Y

Y

S

S

D

D

G

G

F

F

G

G

P

P

K

K

P

P

A

A

D

D

I

I

V

I

H

H

I

V

P

P

F

F

N

N

D

D

L

L

Q

H

A

A

V

V

K

K

A

A

V

V

M

M

D

D

H

H

T

T

C

C

A

A

V

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

V

V

T

Q

A

A

T

T

P

P

A

E

F

F

L

L

Q

K

G

G

L

L

R

R

E

D

L

L

C

C

D

D

Q

E

H

H

Q

Q

A

A

L

L

V

V

F

F

D
|
D

E

E

V
|
V

Q
|
Q

C

C

G

G

M

M

G

G

R

R

T

T

G

G

S

D

L

L

F

F

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

I

L

L

T

T

S

S

A

A

K
|
K

A

A

L

L

G

G

F

F

P

P

V

V

S

S

A

A

M

M

L

L

T

T

H

Q

E

E

I

I

A

A

S

S

A

A

F

F

H

-

A

-

G

-

S

-

H

-

G

G

S

S

T
|
T

Y

Y

G

G

N

N

P

P

L

L

A

A

C

C

A

A

V

V

A

A

N

G

A

A

F

F

D

D

L

I

I

I

N

N

T

T

P

P

A

E

V

V

L

L

D

Q

G

G

V

I

S

H

A

T

K

K

R

R

E

Q

L

Q

F

F

V

V

K

Q

H

H

L

L

Q

Q

Q

A

L

I

D

D

A

E

E

Q

F

F

D

D

L

I

F

F

S

S

D

D

I

I

R

R

G

G

M

M

G

G

L

L

I

I

G

G

A

A

E

E

L

L

K

K

P

P

Q

K

H

Y

K

K

G

G

R

R

A

A

R

R

D

D

F

F

L

L

Y

Y

A

A

A

G

A

A

Q

E

A

A

G

G

V

V

M

M

V

V

L

L

N

N

A

A

G

G

P

A

D

D

V

V

M

M

R
|
R

F

F

V

A

P

P

S

S

L

L

I

V

D

E

E

E

Q

A

D

D

I

I

A

H

E

E

G

G

M

M

A

Q

R

R

F

F

A

A

Q

Q

A

A

V

V

A

G

K

K

V

V

I

V

active site: F130 (= F141), E182 (= E193), D215 (= D226), Q218 (= Q229), K244 (= K255), T268 (= T284), R361 (= R377)
binding pyridoxal-5'-phosphate: S96 (= S107), G97 (= G108), T98 (= T109), F130 (= F141), H131 (= H142), E182 (= E193), D215 (= D226), V217 (= V228), Q218 (= Q229), K244 (= K255)

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
60% identity, 99% coverage: 5:405/406 of query aligns to 1:400/401 of 4adbB

query
sites
4adbB

Q

Q

P

P

A

-

I

I

T

T

R

R

A

E

T

N

F

F

D

D

E

E

V

W

I

M

L

I

P

P

I

V

Y

Y

A

A

P

P

A

A

E

P

F

F

I

I

P

P

V

V

K

R

G

G

K

E

G

G

S

S

R

R

V

L

W

W

D

D

Q

Q

G

G

K

K

E

E

Y

Y

V

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

T

N

A

A

L

L

G

G

H

H

C

A

H

H

P

P

A

E

L

L

V

R

A

E

A

A

L

L

H

N

E

E

Q

Q

G

A

E

S

T

K

L

F

W

W

H

H

T

T

S

G

N

N

V

G

F

Y

T

T

N

N

E

E

P

P

A

V

L

L

R

R

L

L

G

A

C

K

K

K

L

L

V

I

E

D

A

A

T

T

F

F

A

A

E

D

R

R

V

V

V

F

F

F

M

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

L

A

A

R

R

H

K

Y

F

A

A

V

H

T

D

R

R

H

Y

S

G

P

S

Y

H

K

K

T

S

K

G

I

I

I

V

A

A

F

F

H

K

N

N

A

A

F
|
F

H
|
H

G

G

R

R

S

T

L

L

F

F

T

T

V

V

S

S

V

A

G

G

Q

Q

P

P

K

A

Y

Y

S

S

D

Q

G

D

F

F

G

A

P

P

K

L

P

P

A

A

D

D

I

I

V

R

H

H

I

A

P

A

F

Y

N

N

D

D

L

I

Q

N

A

S

V

A

K

S

A

A

V

L

M

I

D

D

H

S

T

T

C

C

A

A

V

V

V

I

V

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

V

V

T

V

A

P

A

A

T

S

P

N

A

A

F

F

L

L

Q

Q

G

G

L

L

R

R

E

E

L

L

C

C

D

N

Q

R

H

H

Q

N

A

A

L

L

V

I

F

F

D
|
D

E

E

V
|
V

Q
|
Q

C

T

G

G

M

V

G

G

R

R

T

T

G

G

S

E

L

L

F

Y

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

L

L

L

T

T

S

T

A

A

K
|
K

A

A

L

L

G

G

F

F

P

P

V

V

S

G

A

A

M

L

L

L

T

A

T

T

H

E

E

E

I

C

A

A

S

R

A

V

F

M

H

T

A

V

G

G

S

T

H

H

G

G

S

T

T
|
T

Y

Y

G

G

N

N

P

P

L

L

A

A

C

S

A

A

V

V

A

A

N

G

A

K

A

V

F

L

D

E

L

L

I

I

N

N

T

T

P

P

A

E

V

M

L

L

D

N

G

G

V

V

S

K

A

Q

K

R

R

H

E

D

L

W

F

F

V

V

K

E

H

R

L

L

Q

N

Q

T

L

I

D

N

A

H

E

R

F

Y

D

G

L

L

F

F

S

S

D

E

I

V

R

R

G

G

M

L

G

G

L

L

I

I

G

G

A

C

E

V

L

L

K

N

P

A

Q

D

H

Y

K

A

G

G

R

Q

A

A

R

K

D

Q

F

I

L

S

Y

Q

A

E

A

A

Q

K

A

A

G

G

V

V

M

M

V

V

L

L

N

I

A

A

G

G

P

G

D

N

V

V

M

V

R
|
R

F

F

V

A

P

P

S

A

L

L

I

N

I

V

D

S

E

E

Q

E

D

E

I

V

A

T

E

T

G

G

M

L

A

D

R

R

F

F

A

A

Q

A

A

A

V

C

A

E

K

H

V

F

I

V

N

S

active site: F136 (= F141), E188 (= E193), D221 (= D226), Q224 (= Q229), K250 (= K255), T279 (= T284), R372 (= R377)
binding pyridoxal-5'-phosphate: S102 (= S107), G103 (= G108), A104 (≠ T109), F136 (= F141), H137 (= H142), D221 (= D226), V223 (= V228), Q224 (= Q229), K250 (= K255)

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
60% identity, 99% coverage: 5:405/406 of query aligns to 1:400/400 of 4addA

query
sites
4addA

Q

Q

P

P

A

-

I

I

T

T

R

R

A

E

T

N

F

F

D

D

E

E

V

W

I

M

L

I

P

P

I

V

Y
|
Y

A

A

P

P

A

A

E

P

F

F

I

I

P

P

V

V

K

R

G

G

K

E

G

G

S

S

R

R

V

L

W

W

D

D

Q

Q

G

G

K

K

E

E

Y

Y

V

I

D

D

F

F

A

A

G

G

I

I

A

A

V

V

T

N

A

A

L

L

G

G

H

H

C

A

H

H

P

P

A

E

L

L

V

R

A

E

A

A

L

L

H

N

E

E

Q

Q

G

A

E

S

T

K

L

F

W

W

H

H

T

T

S

G

N

N

V

G

F

Y

T

T

N

N

E

E

P

P

A

V

L

L

R

R

L

L

G

A

C

K

K

K

L

L

V

I

E

D

A

A

T

T

F

F

A

A

E

D

R

R

V

V

V

F

F

F

M

C

N

N

S

S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

L

A

A

R

R

H

K

Y

F

A

A

V

H

T

D

R

R

H

Y

S

G

P

S

Y

H

K

K

T

S

K

G

I

I

I

V

A

A

F

F

H

K

N

N

A

A

F
|
F

H
|
H

G

G

R
|
R

S

T

L

L

F

F

T

T

V

V

S

S

V

A

G

G

Q

Q

P

P

K

A

Y

Y

S

S

D

Q

G

D

F

F

G

A

P

P

K

L

P

P

A

A

D

D

I

I

V

R

H

H

I

A

P

A

F

Y

N

N

D

D

L

I

Q

N

A

S

V

A

K

S

A

A

V

L

M

I

D

D

H

S

T

T

C

C

A

A

V

V

V

I

V

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

V

V

T

V

A

P

A

A

T

S

P

N

A

A

F

F

L

L

Q

Q

G

G

L

L

R

R

E

E

L

L

C

C

D

N

Q

R

H

H

Q

N

A

A

L

L

V

I

F

F

D
|
D

E

E

V
|
V

Q
|
Q

C

T

G

G

M

V

G

G

R

R

T

T

G

G

S

E

L

L

F

Y

A

A

Y

Y

M

M

H

H

Y

Y

G

G

V

V

T

T

P

P

D

D

I

L

L

L

T

T

S

T

A

A

K
|
K

A

A

L

L

G

G

F

F

P

P

V

V

S

G

A

A

M

L

L

L

T

A

T

T

H

E

E

E

I

C

A

A

S

R

A

V

F

M

H

T

A

V

G

G

S

T

H

H

G

G

S

T

T
|
T

Y

Y

G

G

N

N

P

P

L

L

A

A

C

S

A

A

V

V

A

A

N

G

A

K

A

V

F

L

D

E

L

L

I

I

N

N

T

T

P

P

A

E

V

M

L

L

D

N

G

G

V

V

S

K

A

Q

K

R

R

H

E

D

L

W

F

F

V

V

K

E

H

R

L

L

Q

N

Q

T

L

I

D

N

A

H

E

R

F

Y

D

G

L

L

F

F

S

S

D

E

I

V

R

R

G

G

M

L

G

G

L

L

I

I

G

G

A

C

E

V

L

L

K

N

P

A

Q

D

H

Y

K

A

G

G

R

Q

A

A

R

K

D

Q

F

I

L

S

Y

Q

A

E

A

A

Q

K

A

A

G

G

V

V

M

M

V

V

L

L

N

I

A

A

G

G

P

G

D

N

V

V

M

V

R
|
R

F

F

V

A

P

P

S

A

L

L

I

N

I

V

D

S

E

E

Q

E

D

E

I

V

A

T

E

T

G

G

M

L

A

D

R

R

F

F

A

A

Q

A

A

A

V

C

A

E

K

H

V

F

I

V

N

S

active site: F136 (= F141), E188 (= E193), D221 (= D226), Q224 (= Q229), K250 (= K255), T279 (= T284), R372 (= R377)
binding pyridoxal-5'-phosphate: G103 (= G108), A104 (≠ T109), F136 (= F141), H137 (= H142), D221 (= D226), V223 (= V228), K250 (= K255)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (= Y21), F136 (= F141), R139 (= R144)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
43% identity, 95% coverage: 23:406/406 of query aligns to 19:392/393 of 2ordA

query
sites
2ordA

P

P

A

A

E

T

F

F

I

-

P

-

V

V

K

Y

G

G

K

K

G

G

S

S

R

W

V

I

W

Y

D

D

Q

E

Q

K

G

G

K

N

E

A

Y

Y

V

L

D

D

F

F

A

T

G

S

G

G

I

I

A

A

V

V

T

N

A

V

L

L

G

G

H

H

C

S

H

H

P

P

A

R

L

L

V

V

A

E

A

A

L

I

H

K

E

D

Q

Q

G

A

E

E

T

K

L

L

W

I

H

H

T

C

S

S

N

N

V

L

F

F

T

W

N

N

E

R

P

P

A

Q

L

M

R

E

L

L

G

A

C

E

K

L

L

L

V

S

E

K

A

N

T

T

F

F

A

G

E

G

R

K

V

V

V

F

F

F

M

A

N

N

S

T

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

I

K

K

L

I

A

A

R

R

H

K

Y

Y

A

G

V

K

T

K

R

K

H

S

S

E

P

-

Y

K

K

K

T

Y

K

R

I

I

I

L

A

S

F

A

H

H

N

N

A

S

F
|
F

H
|
H

G

G

R

R

S

T

L

L

F

G

T

S

V

L

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

D

K

G

P

F

F

G

E

P

P

K

L

P

V

A

P

D

G

I

F

V

E

H

Y

I

F

P

E

F

F

N

N

D

N

L

V

Q

E

A

D

V

L

K

R

A

R

V

K

M

M

D

S

D

E

H

D

T

V

C

C

A

A

V

V

V

F

V

L

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

S

G

G

V

I

T

V

A

P

A

A

T

T

P

K

A

E

F

F

L

L

Q

E

G

E

L

A

R

R

E

K

L

L

C

C

D

D

Q

E

H

Y

Q

D

A

A

L

L

V

V

F

F

D
|
D

E

E

V
|
V

Q
|
Q

C

C

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

L

F

F

A

A

Y

Y

M

Q

H

K

Y

Y

G

G

V

V

T

V

P

P

D

D

I

V

L

L

T

T

S

T

A

A

K
|
K

A

G

L

L

G

G

F

V

P

P

V

I

S

G

A

A

M

V

L

I

T

-

T

V

H

N

E

E

I

R

A

A

S

N

A

V

F

L

H

E

A

P

G

G

S

D

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

C

A

R

V

A

A

G

N

V

A

T

A

V

F

I

D

K

L

E

I

L

N

T

T

K

P

E

A

G

V

F

L

L

D

E

G

E

V

V

S

E

A

E

K

K

R

G

E

N

L

Y

F

L

V

M

K

K

H

K

L

L

Q

Q

Q

E

L

M

D

K

A

E

E

E

F

Y

D

D

L

V

F

V

S

A

D

D

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

I

G

G

A

I

E

Q

L

F

K

R

P

E

Q

E

H

V

K

S

G

N

R

-

A

-

R

R

D

E

F

V

L

A

Y

T

A

K

A

C

A

F

Q

E

A

N

G

K

V

L

M

L

V

V

L

V

N

P

A

A

G

G

P

N

D

N

V

T

M

I

R
|
R

F

F

V

L

P

P

S

P

L

L

I

T

I

V

D

E

-

Y

-

G

E

E

Q

I

D

D

I

L

A

A

E

-

G

-

M

-

A

-

R

-

F

-

A

V

Q

E

A

T

V

L

A

K

K

K

V

V

I

L

N

Q

G

G

active site: F134 (= F141), E186 (= E193), D219 (= D226), Q222 (= Q229), K248 (= K255), T276 (= T284), R367 (= R377)
binding pyridoxal-5'-phosphate: G102 (= G108), T103 (= T109), F134 (= F141), H135 (= H142), E186 (= E193), D219 (= D226), V221 (= V228), Q222 (= Q229), K248 (= K255)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
43% identity, 95% coverage: 23:406/406 of query aligns to 11:384/385 of Q9X2A5

query
sites
Q9X2A5

P

P

A

A

E

T

F

F

I

-

P

-

V

V

K

Y

G

G

K

K

G

G

S

S

R

W

V

I

W

Y

D

D

Q

E

Q

K

G

G

K

N

E

A

Y

Y

V

L

D

D

F

F

A

T

G

S

G

G

I

I

A

A

V

V

T

N

A

V

L

L

G

G

H

H

C

S

H

H

P

P

A

R

L

L

V

V

A

E

A

A

L

I

H

K

E

D

Q

Q

G

A

E

E

T

K

L

L

W

I

H

H

T

C

S

S

N

N

V

L

F

F

T

W

N

N

E

R

P

P

A

Q

L

M

R

E

L

L

G

A

C

E

K

L

L

L

V

S

E

K

A

N

T

T

F

F

A

G

E

G

R

K

V

V

V

F

F

F

M

A

N

N

S

T

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

I

K

K

L

I

A

A

R

R

H

K

Y

Y

A

G

V

K

T

K

R

K

H

S

S

E

P

-

Y

K

K

K

T

Y

K

R

I

I

I

L

A

S

F

A

H

H

N

N

A

S

F

F

H

H

G

G

R

R

S

T

L

L

F

G

T

S

V

L

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

D

K

G

P

F

F

G

E

P

P

K

L

P

V

A

P

D

G

I

F

V

E

H

Y

I

F

P

E

F

F

N

N

D

N

L

V

Q

E

A

D

V

L

K

R

A

R

V

K

M

M

D

S

D

E

H

D

T

V

C

C

A

A

V

V

V

F

V

L

E

E

P

P

I

I

Q

Q

G

G

E

E

G

S

G

G

V

I

T

V

A

P

A

A

T

T

P

K

A

E

F

F

L

L

Q

E

G

E

L

A

R

R

E

K

L

L

C

C

D

D

Q

E

H

Y

Q

D

A

A

L

L

V

V

F

F

D

D

E

E

V

V

Q

Q

C

C

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

L

F

F

A

A

Y

Y

M

Q

H

K

Y

Y

G

G

V

V

T

V

P

P

D

D

I

V

L

L

T

T

S

T

A

A

K

K

A

G

L

L

G

G

F

V

P

P

V

I

S

G

A

A

M

V

L

I

T

-

T

V

H

N

E

E

I

R

A

A

S

N

A

V

F

L

H

E

A

P

G

G

S

D

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

C

A

R

V

A

A

G

N

V

A

T

A

V

F

I

D

K

L

E

I

L

N

T

T

K

P

E

A

G

V

F

L

L

D

E

G

E

V

V

S

E

A

E

K

K

R

G

E

N

L

Y

F

L

V

M

K

K

H

K

L

L

Q

Q

Q

E

L

M

D

K

A

E

E

E

F

Y

D

D

L

V

F

V

S

A

D

D

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

I

G

G

A

I

E

Q

L

F

K

R

P

E

Q

E

H

V

K

S

G

N

R

-

A

-

R

R

D

E

F

V

L

A

Y

T

A

K

A

C

A

F

Q

E

A

N

G

K

V

L

M

L

V

V

L

V

N

P

A

A

G

G

P

N

D

N

V

T

M

I

R

R

F

F

V

L

P

P

S

P

L

L

I

T

I

V

D

E

-

Y

-

G

E

E

Q

I

D

D

I

L

A

A

E

-

G

-

M

-

A

-

R

-

F

-

A

V

Q

E

A

T

V

L

A

K

K

K

V

V

I

L

N

Q

G

G

GT 94:95 (= GT 108:109) binding pyridoxal 5'-phosphate
T268 (= T284) binding pyridoxal 5'-phosphate

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
41% identity, 97% coverage: 11:404/406 of query aligns to 29:429/429 of P73133

query
sites
P73133

A

A

T

D

F

F

D

D

E

T

V

Y

I

V

L

M

P

N

I

T

Y
|
Y

A

G

P

R

A

F

E

P

F

I

I

A

P

I

V

A

K

R

G

G

K

Q

G

G

S

S

R

T

V

L

W

W

D

D

Q

T

Q

E

G

G

K

K

E

S

Y

Y

V

L

D

D

F

F

A

V

G

A

G

G

I

I

A

A

V

T

T

C

A

T

L

L

G

G

H

H

C

A

H

H

P

P

A

A

L

L

V

V

A

R

A

A

L

V

H

S

E

D

Q

Q

G

I

E

Q

T

K

L

L

W

H

H

H

T

V

S

S

N

N

V

L

F

Y

T

Y

N

I

E

P

P

E

A

Q

L

G

R

E

L

L

G

A

C

K

K

W

L

I

V

V

E

E

A

H

T

S

F

C

A

A

E

D

R

R

V

V

V

F

F

F

M

C

N

N

S
|
S

G
|
G

T
x
A

E

E

A

A

N

N

E

E

T

A

A

A

F

I

K

K

L

L

A

V

R

R

H

K

Y

Y

A

A

V

H

T

T

R

V

H

L

S

D

P

F

Y

L

K

E

T

Q

K

P

I

V

I

I

A

L

F

T

H

A

N

K

A

A

-

S

F

F

H

H

G

G

R
|
R

S

T

L

L

F

A

T

T

V

I

S

T

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

Y

S

Q

D

Q

G

Y

F

F

G

D

P

P

K

L

P

V

A

P

D

G

I

F

V

D

H

Y

I

V

P

P

F

Y

N

N

D

D

L

I

Q

R

A

S

V

L

K

E

A

N

V

K

M

V

D

A

D

D

-

L

-

D

-

E

-

G

-

N

-

S

H

R

T

V

C

A

A

A

V

I

V

F

V

L

E

E

P

P

I

L

Q

Q

G

G

E
|
E

G

G

V

V

T

R

A

P

A

G

T

D

P

L

A

A

F

Y

L

F

Q

K

G

R

L

V

R

R

E

E

L

I

C

C

D

D

Q

Q

H

N

Q

D

A

I

L

L

V

V

F

F

D
|
D

E

E

V

V

Q
|
Q

C

V

G

G

M

V

G

G

R

R

T

T

G

G

S

K

L

L

F

W

A

G

Y

Y

M

E

H

H

Y

L

G

G

V

V

T

E

P

P

D

D

I

I

L

F

T

T

S

S

A

A

K
|
K

A

G

L

L

G

A

G

G

F

V

P

P

V

I

S

G

A

A

M

M

L

M

T

-

T

C

H

K

E

K

I

F

A

C

S

D

A

V

F

F

H

E

A

P

G

G

S

N

H

H

G

A

S

S

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

C

A

A

V

A

A

G

N

L

A

A

A

V

F

L

D

K

L

T

I

I

N

E

T

G

P

D

A

R

V

L

L

L

D

D

G

N

V

V

S

Q

A

A

K

R

R

G

E

E

L

Q

F

L

V

R

K

S

H

G

L

L

Q

A

Q

E

L

I

D

K

A

N

E

Q

F

Y

-

P

D

T

L

L

F

F

S

T

D

E

I

V

R

R

G

G

M

W

G

G

L

L

L

I

I

N

G

G

A

L

E

E

L

I

K

S

P

A

Q

E

H

S

K

S

G

L

R

T

A

S

R

V

D

E

F

I

L

V

Y

K

A

A

A

M

Q

E

A

Q

G

G

V

L

M

L

V

L

L

A

N

P

A

A

G

G

P

P

D

K

V

V

M

L

R
|
R

F

F

V

V

P

P

S

P

L

L

I

V

D

T

E

E

Q

A

D

E

I

I

A

A

E

Q

G

A

M

V

A

E

R

I

F

L

A

R

Q

Q

A

A

V

I

A

A

K

T

V

L

I

V

Y39 (= Y21) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (= S107) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G108) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (≠ T109) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R144) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E198) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D226) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q229) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K255) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T284) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R377) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
42% identity, 95% coverage: 15:398/406 of query aligns to 67:451/457 of Q9M8M7

query
sites
Q9M8M7

E

K

V

V

I

I

L

V

P

G

I

T

Y

Y

A

A

P

R

A

A

E

P

F

V

I

V

P

L

V

S

K

S

G

G

K

K

G

G

S

C

R

K

V

L

W

F

D

D

Q

P

Q

E

G

G

K

K

E

E

Y

Y

V

L

D

D

F

C

A

A

G

S

G

G

I

I

A

A

V

V

T

N

A

A

L

L

G

G

H

H

C

G

H

D

P

P

A

D

L

W

V

L

A

R

A

A

L

V

H

T

E

E

Q

Q

G

A

E

G

T

V

L

L

W

A

H

H

T

V

S

S

N

N

V

V

F

Y

T

Y

N

T

E

I

P

P

A

Q

L

I

R

E

L

L

G

A

C

K

K

R

L

L

V

V

E

A

A

S

T

S

F

F

A

A

E

D

R

R

V

V

V

F

F

F

M

C

N

N

S

S

G

G

T

T

E

E

A

A

N

N

E

E

T

A

A

A

F

I

K

K

L

F

A

S

R

R

H

K

Y

F

A

Q

V

R

T

F

R

T

H

H

S

P

P

E

Y

D

K

K

-

E

-

V

-

A

T

T

K

G

I

F

I

I

A

A

F

F

H

T

N

N

A

S

F

F

H

H

G

G

R

R

S

T

L

L

F

G

T

A

V

L

S

A

V

L

G

T

G

S

Q

K

P

E

K

Q

Y

Y

S

R

D

T

G

P

F

F

G

E

P

P

K

I

P

M

A

P

D

G

I

V

V

T

H

F

I

L

P

E

F

Y

N

G

D

N

L

I

Q

Q

A

A

-

A

V

T

K

D

A

L

V

I

M

R

D

S

D

G

H

K

T

I

C

A

A

A

V

V

V

F

V

V

E

E

P

P

I

I

Q

Q

G

G

E

E

G

G

V

I

T

Y

A

S

A

A

T

T

P

K

A

E

F

F

L

L

Q

Q

G

S

L

L

R

R

E

S

L

A

C

C

D

D

Q

A

H

A

Q

G

A

S

L

L

V

V

F

F

D

D

E

E

V

V

Q

Q

C

C

G

G

M

L

G

G

R

R

T

T

G

G

S

L

L

M

F

W

A

A

Y

Y

M

E

H

A

Y

F

G

G

V

V

T

T

P

P

D

D

I

I

L

M

T

T

S

V

A

A

K

K

A

P

L

L

G

A

G

G

F

L

P

P

V

I

S

G

A

A

M

V

L

L

T

V

T

T

H

E

E

K

I

V

A

A

S

E

A

T

F

I

H

N

A

Y

G

G

S

D

H

H

G

G

S

S

T

T

Y

F

G

A

G

G

N

S

P

P

L

L

A

V

C

C

A

S

V

A

A

A

N

I

A

A

A

V

F

M

D

D

L

K

I

V

N

S

T

K

P

P

A

S

V

F

L

L

D

S

G

S

V

V

S

S

A

N

K

K

-

G

-

R

-

Y

-

F

R

R

E

D

L

L

F

L

V

V

K

K

H

K

L

L

Q

-

L

-

D

-

A

G

E

G

F

N

D

S

L

H

F

V

S

K

D

E

I

V

R

R

G

G

M

E

G

G

L

L

L

I

I

I

G

G

A

V

E

E

L

L

K

D

P

V

Q

P

H

-

K

-

G

-

R

-

A

A

R

S

D

S

F

L

L

V

Y

D

A

A

A

C

A

R

Q

D

A

S

G

G

V

L

M

L

V

I

L

L

N

T

A

A

G

G

P

K

-

G

D

N

V

V

M

V

R

R

F

I

V

V

P

P

S

P

L

L

I

V

I

I

D

S

E

E

Q

E

D

E

I

I

A

E

E

R

G

A

M

V

A

E

R

I

F

M

A

S

Q

Q

Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
40% identity, 92% coverage: 23:394/406 of query aligns to 12:370/376 of O66442

query
sites
O66442

P

P

A

V

E

K

F

F

I

-

P

-

V

V

K

R

G

G

K

K

G

G

S

V

R

Y

V

L

W

Y

D

D

Q

E

G

G

K

K

E

E

Y

Y

V

L

D

D

F

F

A

V

G

S

G

G

I

I

A

G

V

V

T

N

A

S

L

L

G

G

H

H

C

A

H

Y

P

P

A

K

L

L

V

T

A

E

A

A

L

L

H

K

E

E

Q

Q

G

V

E

E

T

K

L

L

W

L

H

H

T

V

S

S

N

N

V

L

F

Y

T

E

N

N

E

P

P

W

A

Q

L

E

R

E

L

L

G

A

C

H

K

K

L

L

V

V

E

K

A

H

T

F

F

W

A

T

E

E

-

G

R

K

V

V

V

F

F

F

M

A

N

N

S

S

G
|
G

T
|
T

E

E

A

S

N

V

E

E

T

A

A

A

F

I

K

K

L

L

A

A

R

R

H

K

Y

Y

A

W

V

R

T

D

R

K

H

-

S

G

P

K

Y

N

K

K

T

W

K

K

I

F

I

I

A

S

F

F

H

E

N

N

A

S

F

F

H

H

G

G

R

R

S

T

L

Y

F

G

T

S

V

L

S

S

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

F

S

H

D

K

G

G

F

F

G

E

P

P

K

L

P

V

A

P

D

G

I

F

V

S

H

Y

I

A

P

K

F

L

N

N

D

D

L

I

Q

D

A

S

V

V

K

Y

A

K

V

L

M

L

D

D

D

E

H

E

T

T

C

A

A

G

V

I

E

E

P

V

I

I

Q

Q

G

G

E

E

G

G

V

V

T

N

A

E

A

A

T

S

P

E

A

D

F

F

L

L

Q

S

G

K

L

L

R

Q

E

E

L

I

C

C

D

K

Q

E

H

K

Q

D

A

V

L

L

V

I

F

I

D

D

E

E

V

V

Q

Q

C

T

G

G

M

I

G

G

R

R

T

T

G

G

S

E

L

F

F

Y

A

A

Y

Y

M

Q

H

H

Y

F

G

N

V

L

T

K

P

P

D

D

I

V

L

I

T

A

S

L

A

A

K
|
K

A

G

L

L

G

G

F

V

P

P

V

I

S

G

A

A

M

I

L

L

T

A

T

R

H

E

E

E

I

V

A

A

S

Q

A

S

F

F

H

T

A

P

G

G

S

S

H

H

G

G

S

S

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

C

A

R

V

A

A

G

N

T

A

V

F

V

D

D

L

E

I

V

N

E

T

K

P

-

A

-

V

L

L

L

D

P

G

H

V

V

S

R

A

E

K

V

R

G

E

N

L

Y

F

F

V

K

K

E

H

K

L

L

Q

K

Q

E

L

L

D

G

A

K

E

-

F

-

D

-

L

-

F

-

S

G

D

K

I

V

R

K

G

G

M

R

G

G

L

L

L

M

I

L

G

G

A

L

E

E

L

L

K

E

P

R

Q

E

H

-

K

-

G

-

R

-

A

C

R

K

D

D

F

Y

L

V

Y

L

A

K

A

A

A

L

Q

E

A

K

G

G

V

L

M

L

V

I

L

N

N

C

A

T

G

A

P

G

D

K

V

V

M

L

R

R

F

F

V

L

P

P

S

P

L

L

I

I

D

Q

E

K

Q

E

D

H

I

I

A

D

E

R

G

A

M

I

A

S

GT 96:97 (= GT 108:109) binding pyridoxal 5'-phosphate
K242 (= K255) modified: N6-(pyridoxal phosphate)lysine
T271 (= T284) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
40% identity, 92% coverage: 23:394/406 of query aligns to 11:369/375 of 2eh6A

query
sites
2eh6A

P

P

A

V

E

K

F

F

I

-

P

-

V

V

K

R

G

G

K

K

G

G

S

V

R

Y

V

L

W

Y

D

D

Q

E

G

G

K

K

E

E

Y

Y

V

L

D

D

F

F

A

V

G

S

G

G

I

I

A

G

V

V

T

N

A

S

L

L

G

G

H

H

C

A

H

Y

P

P

A

K

L

L

V

T

A

E

A

A

L

L

H

K

E

E

Q

Q

G

V

E

E

T

K

L

L

W

L

H

H

T

V

S

S

N

N

V

L

F

Y

T

E

N

N

E

P

P

W

A

Q

L

E

R

E

L

L

G

A

C

H

K

K

L

L

V

V

E

K

A

H

T

F

F

W

A

T

E

E

-

G

R

K

V

V

V

F

F

F

M

A

N

N

S

S

G
|
G

T
|
T

E

E

A

S

N

V

E

E

T

A

A

A

F

I

K

K

L

L

A

A

R

R

H

K

Y

Y

A

W

V

R

T

D

R

K

H

-

S

G

P

K

Y

N

K

K

T

W

K

K

I

F

I

I

A

S

F

F

H

E

N

N

A

S

F
|
F

H
|
H

G

G

R

R

S

T

L

Y

F

G

T

S

V

L

S

S

V

A

G

T

G

G

Q

Q

P

P

K

K

Y

F

S

H

D

K

G

G

F

F

G

E

P

P

K

L

P

V

A

P

D

G

I

F

V

S

H

Y

I

A

P

K

F

L

N

N

D

D

L

I

Q

D

A

S

V

V

K

Y

A

K

V

L

M

L

D

D

D

E

H

E

T

T

C

A

A

G

V

I

E
|
E

P

V

I

I

Q

Q

G

G

E

E

G

G

V

V

T

N

A

E

A

A

T

S

P

E

A

D

F

F

L

L

Q

S

G

K

L

L

R

Q

E

E

L

I

C

C

D

K

Q

E

H

K

Q

D

A

V

L

L

V

I

F

I

D
|
D

E

E

V
|
V

Q
|
Q

C

T

G

G

M

I

G

G

R

R

T

T

G

G

S

E

L

F

F

Y

A

A

Y

Y

M

Q

H

H

Y

F

G

N

V

L

T

K

P

P

D

D

I

V

L

I

T

A

S

L

A

A

K
|
K

A

G

L

L

G

G

F

V

P

P

V

I

S

G

A

A

M

I

L

L

T

A

T

R

H

E

E

E

I

V

A

A

S

Q

A

S

F

F

H

T

A

P

G

G

S

S

H

H

G

G

S

S

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

C

A

R

V

A

A

G

N

T

A

V

F

V

D

D

L

E

I

V

N

E

T

K

P

-

A

-

V

L

L

L

D

P

G

H

V

V

S

R

A

E

K

V

R

G

E

N

L

Y

F

F

V

K

K

E

H

K

L

L

Q

K

Q

E

L

L

D

G

A

K

E

-

F

-

D

-

L

-

F

-

S

G

D

K

I

V

R

K

G

G

M

R

G

G

L

L

L

M

I

L

G

G

A

L

E

E

L

L

K

E

P

R

Q

E

H

-

K

-

G

-

R

-

A

C

R

K

D

D

F

Y

L

V

Y

L

A

K

A

A

A

L

Q

E

A

K

G

G

V

L

M

L

V

I

L

N

N

C

A

T

G

A

P

G

D

K

V

V

M

L

R
|
R

F

F

V

L

P

P

S

P

L

L

I

I

D

Q

E

K

Q

E

D

H

I

I

A

D

E

R

G

A

M

I

A

S

active site: F127 (= F141), E179 (= E193), D212 (= D226), Q215 (= Q229), K241 (= K255), T270 (= T284), R352 (= R377)
binding pyridoxal-5'-phosphate: G95 (= G108), T96 (= T109), F127 (= F141), H128 (= H142), E179 (= E193), D212 (= D226), V214 (= V228), K241 (= K255)

3nx3A Crystal structure of acetylornithine aminotransferase (argd) from campylobacter jejuni
38% identity, 95% coverage: 17:400/406 of query aligns to 4:385/388 of 3nx3A

query
sites
3nx3A

I

I

L

I

P

P

I

T

Y

Y

A

K

P

R

A

F

E

D

F

I

I

V

P

L

V

E

K

K

G

G

K

Q

G

G

S

V

R

Y

V

L

W

F

D

D

Q

D

Q

K

G

A

K

K

E

K

Y

Y

V

L

D

D

F

F

A

S

G

S

G

G

I

I

A

G

V

V

T

C

A

A

L

L

G

G

H

Y

C

N

H

H

P

A

A

K

L

F

V

N

A

A

A

K

L

I

H

K

E

A

Q

Q

G

V

E

D

T

K

L

L

W

L

H

H

T

T

S

S

N

N

V

L

F

Y

T

Y

N

N

E

E

P

N

A

I

L

A

R

A

L

A

G

A

C

K

K

N

L

L

V

A

E

K

A

A

T

S

F

A

A

L

E

E

R

R

V

V

V

F

F

F

M

T

N

N

S

S

G

G

T

T

E

E

A

S

N

I

E

E

T

G

A

A

F

M

K

K

L

T

A

A

R

R

H

K

Y

Y

A

A

V

F

T

N

R

K

H

G

S

V

P

K

Y

-

K

G

T

G

K

Q

I

F

I

I

A

A

F

F

H

K

N

H

A

S

F
|
F

H

H

G

G

R

R

S

T

L

L

F

G

T

A

V

L

S

S

V

L

G

T

G

A

Q

N

P

E

K

K

Y

Y

S

Q

D

K

G

P

F

F

G

K

P

P

K

L

P

I

A

S

D

G

I

V

V

K

H

F

I

A

P

K

F

Y

N

N

D

D

L

I

Q

S

A

S

V

V

K

E

A

K

V

L

M

V

D

N

D

E

H

K

T

T

C

C

A

A

V

I

V

L

E
|
E

P

S

I

V

Q

Q

G

G

E

E

G

G

V

I

T

N

A

P

A

A

T
x
N

P

K

A

D

F
|
F

L

Y

Q

K

G

A

L

L

R

R

E

K

L

L

C

C

D

D

Q

E

H

K

Q

D

A

I

L

L

V

I

F

A

D
|
D

E

E

V

I

Q
|
Q

C

C

G

G

M

M

G

G

R

R

T

S

G

G

S

K

L

F

F

F

A

A

Y

Y

M

E

H

H

Y

A

G

Q

V

I

T

L

P

P

D

D

I

I

L

M

T

T

S

S

A

A

K
|
K

A

A

L

L

G

G

G

C

G

G

F

L

P

S

V

V

S

G

A

A

M

F

L

V

T

I

T

N

H

Q

E

K

I

V

A

A

S

S

-

N

A

S

F

L

H

E

A

A

G

G

S

D

H

H

G

G

S

S

T
|
T

Y

Y

G

G

N

N

P

P

L

L

A

V

C

C

A

A

V

G

A

V

N

N

A

A

A

V

F

F

D

E

L

I

I

F

N

K

T

E

P

E

A

K

V

I

L

L

D

E

G

N

V

V

S

N

A

K

K

L

R

T

E

P

L

Y

F

L

V

E

K

Q

H

S

L

L

Q

D

Q

E

L

L

D
x
I

A

N

E

E

F
|
F

D
|
D

L

F

F
x
C

S

K

D

K

I

R

R

K

G

G

M

L

G

G

L

F

L

M

I

Q

G

G

A

L

E

S

L

L

K

D

P

K

Q

S

H

V

K

K

G

-

R

-

A

V

R

A

D

K

F

V

L

I

Y

Q

A

K

A

C

A

Q

Q

E

A

N

G

A

V

L

M

L

V

L

L

I

N

S

A

C

G

G

P

E

D

N

V

D

M

L

R
|
R

F

F

V

L

P

P

S

P

L

L

I

I

I

L

D
x
Q

E
x
K

Q

E

D

H

I

I

A

D

E

E

G

M

M

S

A

E

R

K

F

L

A

R

Q

K

A

A

V

L

active site: F127 (= F141), E179 (= E193), D212 (= D226), Q215 (= Q229), K241 (= K255), T271 (= T284), R362 (= R377)
binding magnesium ion: N191 (≠ T205), F194 (= F208), I313 (≠ D326), F316 (= F329), D317 (= D330), C319 (≠ F332), Q370 (≠ D385), K371 (≠ E386)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
39% identity, 95% coverage: 20:404/406 of query aligns to 23:394/395 of Q5SHH5

query
sites
Q5SHH5

I

V

Y

Y

A

N

P

K

A

H

E

D

F

L

I

L

P

I

V

V

K

R

G

G

K

Q

G

G

S

A

R

R

V

V

W

W

D

D

Q

A

Q

E

G

G

K

N

E

E

Y

Y

V

I

D

D

F

C

A

V

G

G

I

Y

A

G

V

V

T

A

A

N

L

L

G

G

H

H

C

G

H

N

P

P

A

E

L

V

V

V

A

E

A

A

L

V

H

K

E

R

Q

Q

G

A

E

E

T

T

L

L

W

M

H

A

T

M

S

P

N

Q

V

T

F

L

T

P

N

T

E

-

P

P

A

M

L

R

R

G

L

E

G

F

C

Y

K

R

L

T

V

L

E

T

A

A

T

I

F

L

A

P

-

P

-

E

-

L

E

N

R

R

V

V

V

F

F

P

M

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

F

A

A

R

R

H

A

Y

H

A

T

V

-

T

-

R

-

H

-

S

-

P

-

Y

G

K

R

T

K

K

K

I

F

I

V

A

A

F

A

H

M

N

R

A

G

F

F

H

S

G

G

R

R

S

T

L

M

F

G

T

S

V

L

S

S

V

V

G

T

G

W

Q

E

P

P

K

K

Y

Y

S

R

D

E

G

P

F

F

G

L

P

P

K

L

P

V

A

E

D

P

I

V

V

E

H

F

I

I

P

P

F

Y

N

N

D

D

L

V

Q

E

A

A

V

L

K

K

A

R

V

A

M

V

D

D

D

E

H

E

T

T

C

A

A

A

V

V

V

I

V

L

E

E

P

P

I

V

Q

Q

G

G

E

E

G

G

V

V

T

R

A

P

A

A

T

T

P

P

A

E

F

F

L

L

Q

R

G

A

L

A

R

R

E

E

L

I

C

T

D

Q

Q

E

H

K

Q

G

A

A

L

L

V

I

F

L

D

D

E

E

V

I

Q

Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

R

F

F

A

A

Y

F

M

E

H

H

Y

F

G

G

V

I

T

V

P

P

D

D

I

I

L

L

T

T

S

L

A

A

K
|
K

A

A

L

L

G

G

F

V

P

P

V

L

S

G

A

A

M

A

L

V

T

M

T

R

H

E

E

E

I

V

A

A

S

R

A

S

F

M

H

P

A

K

G

G

S

G

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

M

A

A

V

A

A

G

N

V

A

A

F

I

D

R

L

Y

I

L

N

E

T

R

P

T

A

R

V

L

L

W

D

E

G

R

V

A

S

A

A

E

K

L

R

G

E

P

L

W

F

F

V

M

K

E

H

K

L

L

Q

R

Q

A

L

I

D

P

A

S

E

-

F

-

D

P

L

K

F

I

S

R

D

E

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

V

G

G

A

L

E

E

L

L

K

K

P

E

Q

K

H

A

K

-

G

-

R

-

A

A

R

P

D

Y

F

I

L

A

Y

R

A

L

A

E

A

K

Q

E

A

H

G

R

V

V

M

L

V

A

L

L

N

Q

A

A

G

G

P

P

D

T

V

V

M

I

R

R

F

F

V

L

P

P

S

P

L

L

I

V

I

I

D

E

E

K

Q

E

D

D

I

-

A

-

E

-

G

-

M

L

A

E

R

R

F

V

A

V

Q

E

A

A

V

V

A

R

K

A

V

V

I

L

GT 113:114 (= GT 108:109) binding pyridoxal 5'-phosphate
K254 (= K255) modified: N6-(pyridoxal phosphate)lysine
T283 (= T284) binding pyridoxal 5'-phosphate

P9WPZ7 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
41% identity, 96% coverage: 2:389/406 of query aligns to 4:382/400 of P9WPZ7

query
sites
P9WPZ7

A

A

T

S

E

T

Q

T

P

T

A

A

I

T

T

M

R

R

A

Q

T

R

F

W

D

Q

E

A

V

V

I

M

L

M

P

N

I

N

Y

Y

A

G

P

T

A

P

E

P

F

I

I

A

P

L

V

A

K

S

G

G

K

D

G

G

S

A

R

V

V

V

W

T

D

D

Q

V

Q

D

G

G

K

R

E

T

Y

Y

V

I

D

D

F

L

A

L

G

G

I

I

A

A

V

V

T

N

A

V

L

L

G

G

H

H

C

R

H

H

P

P

A

A

L

V

V

I

A

E

A

A

L

V

H

T

E

R

Q

Q

G

M

E

S

T

T

L

L

W

G

H

H

T

T

S

S

N

N

V

L

F

Y

T

A

N

T

E

E

P

P

A

G

L

I

R

A

L

L

G

A

C

E

K

E

L

L

V

V

E

A

A

L

T

L

F

G

A

A

E

D

-

Q

-

R

-

T

R

R

V

V

V

F

F

F

M

C

N

N

S

S

G

G

T

A

E

E

A

A

N

N

E

E

T

A

A

A

F

F

K

K

L

L

A

S

R

R

H

L

Y

T

A

G

V

-

T

-

R

-

H

-

S

-

P

-

Y

-

K

R

T

T

K

K

I

L

I

V

A

A

F

A

H

H

N

D

A

A

F

F

H

H

G

G

R

R

S

T

L

M

F

G

T

S

V

L

S

A

V

L

G

T

G

G

Q

Q

P

P

K

A

Y

K

S

Q

D

T

G

P

F

F

G

A

P

P

K

L

P

P

A

G

D

D

I

V

V

T

H

H

I

V

P

G

F

Y

N

G

D

D

L

V

Q

D

A

A

V

L

K

A

A

A

V

A

M

V

D

D

H

H

T

T

C

A

A

A

V

V

V

F

V

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

V

V

T

V

A

V

A

P

T

P

P

A

A

G

F

Y

L

L

Q

A

G

A

L

A

R

R

E

D

L

I

C

T

D

A

Q

R

H

R

Q

G

A

A

L

L

V

V

F

L

D

D

E

E

V

V

Q

Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

A

L

F

F

F

A

A

Y

H

M

Q

H

H

Y

D

G

G

V

I

T

T

P

P

D

D

I

V

L

V

T

T

S

L

A

A

K

K

A

G

L

L

G

G

F

L

P

P

V

I

S

G

A

A

M

C

L

L

T

A

T

V

H

G

E

P

I

A

A

A

S

E

A

L

F

L

H

T

A

P

G

G

S

L

H

H

G

G

S

S

T

T

Y

F

G

G

N

N

P

P

L

V

A

C

C

A

A

A

V

A

A

A

N

L

A

A

A

V

F

L

D

R

L

V

I

L

N

A

T

S

P

-

A

-

V

-

L

-

D

D

G

G

V

L

S

V

A

R

K

R

R

A

E

E

L

V

F

L

V

G

K
|
K

H

S

L

L

Q

R

Q

H

-

G

L

I

D

E

A

A

-

L

E

G

F

H

D

P

L

L

F

I

S

D

D

H

I

V

R

R

G

G

M

R

G

G

L

L

I

L

G

G

A

I

E

A

L

L

K

T

P

A

Q

P

H

H

K

-

G

-

R

-

A

A

R

K

D

D

F

A

L

E

Y

A

A

T

A

A

A

R

Q

D

A

A

G

G

V

Y

M

L

V

V

L

N

N

A

A

A

G

A

P

P

D

D

V

V

M

I

R

R

F

L

V

A

P

P

S

P

L

L

I

I

D

A

E

E

Q

A

D

Q

I

L

K314 (= K320) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine; partial

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 95% coverage: 20:404/406 of query aligns to 15:386/387 of 1wkhA

query
sites
1wkhA

I

V

Y

Y

A

N

P

K

A

H

E

D

F

L

I

L

P

I

V

V

K

R

G

G

K

Q

G

G

S

A

R

R

V

V

W

W

D

D

Q

A

Q

E

G

G

K

N

E

E

Y

Y

V

I

D

D

F

C

A

V

G

G

I
x
Y

A

G

V

V

T

A

A

N

L

L

G

G

H

H

C

G

H

N

P

P

A

E

L

V

V

V

A

E

A

A

L

V

H

K

E

R

Q

Q

G

A

E

E

T

T

L

L

W

M

H

A

T

M

S

P

N

Q

V

T

F

L

T

P

N

T

E

P

P

M

A

R

L

G

R

E

L

F

G

Y

C

R

K

T

L

L

V

T

E

A

A

I

T

L

F

P

A

P

E

E

-

L

-

N

R

R

V

V

V

F

F

P

M

V

N

N

S
|
S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

F

A

A

R

R

H

A

Y

H

A

T

V

-

T

-

R

-

H

-

S

-

P

-

Y

G

K

R

T

K

K

K

I

F

I

V

A

A

F

A

H

M

N

R

A

G

F
|
F

H
x
S

G

G

R

R

S

T

L

M

F

G

T

S

V

L

S

S

V

V

G

T

G

W

Q

E

P

P

K

K

Y

Y

S

R

D

E

G

P

F

F

G

L

P

P

K

L

P

V

A

E

D

P

I

V

V

E

H

F

I

I

P

P

F

Y

N

N

D

D

L

V

Q

E

A

A

V

L

K

K

A

R

V

A

M

V

D

D

D

E

H

E

T

T

C

A

A

A

V

V

V

I

V

L

E
|
E

P

P

I

V

Q

Q

G

G

E
|
E

G

G

V

V

T

R

A

P

A

A

T

T

P

P

A

E

F

F

L

L

Q

R

G

A

L

A

R

R

E

E

L

I

C

T

D

Q

Q

E

H

K

Q

G

A

A

L

L

V

I

F

L

D
|
D

E

E

V
x
I

Q
|
Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

R

F

F

A

A

Y

F

M

E

H

H

Y

F

G

G

V

I

T

V

P

P

D

D

I

I

L

L

T

T

S

L

A

A

K
|
K

A

A

L

L

G

G

F

V

P

P

V

L

S

G

A

V

M

A

L

V

T

M

T

R

H

E

E

E

I

V

A

A

S

R

A

S

F

M

H

P

A

K

G

G

S

G

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

M

A

A

V

A

A

G

N

V

A

A

F

I

D

R

L

Y

I

L

N

E

T

R

P

T

A

R

V

L

L

W

D

E

G

R

V

A

S

A

A

E

K

L

R

G

E

P

L

W

F

F

V

M

K

E

H

K

L

L

Q

R

Q

A

L

I

D

P

A

S

E

-

F

-

D

P

L

K

F

I

S

R

D

E

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

V

G

G

A

L

E

E

L

L

K

K

P

E

Q

K

H

A

K

-

G

-

R

-

A

A

R

P

D

Y

F

I

L

A

Y

R

A

L

A

E

A

K

Q

E

A

H

G

R

V

V

M

L

V

A

L

L

N

Q

A

A

G

G

P

P

D

T

V

V

M

I

R
|
R

F

F

V

L

P

P

S

P

L

L

I

V

I

I

D

E

E

K

Q

E

D

D

I

-

A

-

E

-

G

-

M

L

A

E

R

R

F

V

A

V

Q

E

A

A

V

V

A

R

K

A

V

V

I

L

active site: F132 (= F141), E184 (= E193), D217 (= D226), Q220 (= Q229), K246 (= K255), T275 (= T284), R363 (= R377)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I51), S104 (= S107), G105 (= G108), T106 (= T109), F132 (= F141), S133 (≠ H142), E184 (= E193), E189 (= E198), D217 (= D226), I219 (≠ V228), K246 (= K255), R363 (= R377)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 95% coverage: 20:404/406 of query aligns to 15:386/387 of 1wkgA

query
sites
1wkgA

I

V

Y
|
Y

A

N

P

K

A

H

E

D

F

L

I

L

P

I

V

V

K

R

G

G

K

Q

G

G

S

A

R

R

V

V

W

W

D

D

Q

A

Q

E

G

G

K

N

E

E

Y

Y

V

I

D

D

F

C

A

V

G

G

I
x
Y

A

G

V

V

T

A

A

N

L

L

G

G

H

H

C

G

H

N

P

P

A

E

L

V

V

V

A

E

A

A

L

V

H

K

E

R

Q

Q

G

A

E

E

T

T

L

L

W

M

H

A

T

M

S

P

N

Q

V

T

F

L

T

P

N

T

E

P

P

M

A

R

L

G

R

E

L

F

G

Y

C

R

K

T

L

L

V

T

E

A

A

I

T

L

F

P

A

P

E

E

-

L

-

N

R

R

V

V

V

F

F

P

M

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

F

A

A

R

R

H

A

Y

H

A

T

V

-

T

-

R

-

H

-

S

-

P

-

Y

G

K

R

T

K

K

K

I

F

I

V

A

A

F

A

H

M

N

R

A

G

F
|
F

H
x
S

G

G

R
|
R

S

T

L

M

F

G

T

S

V

L

S

S

V

V

G

T

G

W

Q

E

P

P

K

K

Y

Y

S

R

D

E

G

P

F

F

G

L

P

P

K

L

P

V

A

E

D

P

I

V

V

E

H

F

I

I

P

P

F

Y

N

N

D

D

L

V

Q

E

A

A

V

L

K

K

A

R

V

A

M

V

D

D

D

E

H

E

T

T

C

A

A

A

V

V

V

I

V

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

V

V

T

R

A

P

A

A

T

T

P

P

A

E

F

F

L

L

Q

R

G

A

L

A

R

R

E

E

L

I

C

T

D

Q

Q

E

H

K

Q

G

A

A

L

L

V

I

F

L

D
|
D

E

E

V
x
I

Q
|
Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

R

F

F

A

A

Y

F

M

E

H

H

Y

F

G

G

V

I

T

V

P

P

D

D

I

I

L

L

T

T

S

L

A

A

K
|
K

A

A

L

L

G

G

F

V

P

P

V

L

S

G

A

V

M

A

L

V

T

M

T

R

H

E

E

E

I

V

A

A

S

R

A

S

F

M

H

P

A

K

G

G

S

G

H

H

G
|
G

S
x
T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

M

A

A

V

A

A

G

N

V

A

A

F

I

D

R

L

Y

I

L

N

E

T

R

P

T

A

R

V

L

L

W

D

E

G

R

V

A

S

A

A

E

K

L

R

G

E

P

L

W

F

F

V

M

K

E

H

K

L

L

Q

R

Q

A

L

I

D

P

A

S

E

-

F

-

D

P

L

K

F

I

S

R

D

E

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

V

G

G

A

L

E

E

L

L

K

K

P

E

Q

K

H

A

K

-

G

-

R

-

A

A

R

P

D

Y

F

I

L

A

Y

R

A

L

A

E

A

K

Q

E

A

H

G

R

V

V

M

L

V

A

L

L

N

Q

A

A

G

G

P

P

D

T

V

V

M

I

R
|
R

F

F

V

L

P

P

S

P

L

L

I

V

I

I

D

E

E

K

Q

E

D

D

I

-

A

-

E

-

G

-

M

L

A

E

R

R

F

V

A

V

Q

E

A

A

V

V

A

R

K

A

V

V

I

L

active site: F132 (= F141), E184 (= E193), D217 (= D226), Q220 (= Q229), K246 (= K255), T275 (= T284), R363 (= R377)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y21), Y46 (≠ I51), G105 (= G108), T106 (= T109), F132 (= F141), S133 (≠ H142), R135 (= R144), E184 (= E193), D217 (= D226), I219 (≠ V228), Q220 (= Q229), K246 (= K255), G273 (= G282), T274 (≠ S283), T275 (= T284)

Sites not aligning to the query:

active site: 13

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
39% identity, 95% coverage: 20:404/406 of query aligns to 15:386/387 of 1vefA

query
sites
1vefA

I

V

Y

Y

A

N

P

K

A

H

E

D

F

L

I

L

P

I

V

V

K

R

G

G

K

Q

G

G

S

A

R

R

V

V

W

W

D

D

Q

A

Q

E

G

G

K

N

E

E

Y

Y

V

I

D

D

F

C

A

V

G

G

I

Y

A

G

V

V

T

A

A

N

L

L

G

G

H

H

C

G

H

N

P

P

A

E

L

V

V

V

A

E

A

A

L

V

H

K

E

R

Q

Q

G

A

E

E

T

T

L

L

W

M

H

A

T

M

S

P

N

Q

V

T

F

L

T

P

N

T

E

P

P

M

A

R

L

G

R

E

L

F

G

Y

C

R

K

T

L

L

V

T

E

A

A

I

T

L

F

P

A

P

E

E

-

L

-

N

R

R

V

V

V

F

F

P

M

V

N

N

S

S

G
|
G

T
|
T

E

E

A

A

N

N

E

E

T

A

A

A

F

L

K

K

L

F

A

A

R

R

H

A

Y

H

A

T

V

-

T

-

R

-

H

-

S

-

P

-

Y

G

K

R

T

K

K

K

I

F

I

V

A

A

F

A

H

M

N

R

A

G

F
|
F

H
x
S

G

G

R

R

S

T

L

M

F

G

T

S

V

L

S

S

V

V

G

T

G

W

Q

E

P

P

K

K

Y

Y

S

R

D

E

G

P

F

F

G

L

P

P

K

L

P

V

A

E

D

P

I

V

V

E

H

F

I

I

P

P

F

Y

N

N

D

D

L

V

Q

E

A

A

V

L

K

K

A

R

V

A

M

V

D

D

D

E

H

E

T

T

C

A

A

A

V

V

V

I

V

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

V

V

T

R

A

P

A

A

T

T

P

P

A

E

F

F

L

L

Q

R

G

A

L

A

R

R

E

E

L

I

C

T

D

Q

Q

E

H

K

Q

G

A

A

L

L

V

I

F

L

D
|
D

E

E

V
x
I

Q

Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

K

L

R

F

F

A

A

Y

F

M

E

H

H

Y

F

G

G

V

I

T

V

P

P

D

D

I

I

L

L

T

T

S

L

A

A

K
|
K

A

A

L

L

G

G

F

V

P

P

V

L

S

G

A

V

M

A

L

V

T

M

T

R

H

E

E

E

I

V

A

A

S

R

A

S

F

M

H

P

A

K

G

G

S

G

H

H

G

G

S

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

A

A

C

M

A

A

V

A

A

G

N

V

A

A

F

I

D

R

L

Y

I

L

N

E

T

R

P

T

A

R

V

L

L

W

D

E

G

R

V

A

S

A

A

E

K

L

R

G

E

P

L

W

F

F

V

M

K

E

H

K

L

L

Q

R

Q

A

L

I

D

P

A

S

E

-

F

-

D

P

L

K

F

I

S

R

D

E

I

V

R

R

G

G

M

M

G

G

L

L

L

M

I

V

G

G

A

L

E

E

L

L

K

K

P

E

Q

K

H

A

K

-

G

-

R

-

A

A

R

P

D

Y

F

I

L

A

Y

R

A

L

A

E

A

K

Q

E

A

H

G

R

V

V

M

L

V

A

L

L

N

Q

A

A

G

G

P

P

D

T

V

V

M

I

R
|
R

F

F

V

L

P

P

S

P

L

L

I

V

I

I

D

E

E

K

Q

E

D

D

I

-

A

-

E

-

G

-

M

L

A

E

R

R

F

V

A

V

Q

E

A

A

V

V

A

R

K

A

V

V

I

L

active site: F132 (= F141), D217 (= D226), K246 (= K255), T275 (= T284), R363 (= R377)
binding pyridoxal-5'-phosphate: G105 (= G108), T106 (= T109), F132 (= F141), S133 (≠ H142), E184 (= E193), D217 (= D226), I219 (≠ V228), K246 (= K255)

Sites not aligning to the query:

active site: 13

7nncC Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal-5'-phosphate and 6-methoxyquinoline-3-carboxylic acid
41% identity, 94% coverage: 7:389/406 of query aligns to 3:376/391 of 7nncC

query
sites
7nncC

A

A

I

T

T

M

R

R

A

Q

T

R

F

W

D

Q

E

A

V

V

I

M

L

M

P

N

I

N

Y

Y

A

G

P

T

A

P

E

P

F

I

I

A

P

L

V

A

K

S

G

G

K

D

G

G

S

A

R

V

V

V

W

T

D

D

Q

V

Q

D

G

G

K

R

E

T

Y

Y

V

I

D

D

F

L

A

L

G

G

I

I

A

A

V

V

T

N

A

V

L

L

G

G

H

H

C

R

H

H

P

P

A

A

L

V

V

I

A

E

A

A

L

V

H

T

E

R

Q

Q

G

M

E

S

T

T

L

L

W

G

H

H

T

T

S

S

N
|
N

V
x
L

F

Y

T

A

N

T

E

E

P

P

A

G

L

I

R

A

L

L

G

A

C

E

K

E

L

L

V

V

E

A

A

L

T

L

F

G

A

A

E

D

-

Q

-

R

-

T

R

R

V

V

V

F

F

F

M

C

N

N

S

S

G

G

T

A

E

E

A

A

N

N

E

E

T

A

A

A

F

F

K

K

L

L

A

S

R

R

H

L

Y

T

A

G

V

-

T

-

R

-

H

-

S

-

P

-

Y

-

K

R

T

T

K

K

I

L

I

V

A

A

F

A

H

H

N

D

A

A

F
|
F

H

H

G

G

R

R

S

T

L

M

F

G

T

S

V

L

S

A

V

L

G

T

G

G

Q

Q

P

P

K

A

Y

K

S

Q

D

T

G

P

F

F

G

A

P

P

K

L

P

P

A

G

D

D

I

V

V

T

H

H

I

V

P

G

F

Y

N

G

D

D

L

V

Q

D

A

A

V

L

K

A

A

A

V

A

M

V

D

D

H

H

T

T

C

A

A

A

V

V

V

F

V

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

V

V

T

V

A

V

A

P

T

P

P

A

A

G

F

Y

L

L

Q

A

G

A

L

A

R

R

E

D

L

I

C

T

D

A

Q

R

H

R

Q

G

A

A

L

L

V

V

F

L

D
|
D

E

E

V

V

Q

Q

C

T

G

G

M

M

G

G

R

R

T

T

G

G

S

A

L

F

F

F

A

A

Y

H

M

Q

H

H

Y

D

G

G

V

I

T

T

P

P

D

D

I

V

L

V

T

T

S

L

A

A

K
|
K

A

G

L

L

G

G

F

L

P

P

V

I

S

G

A

A

M

C

L

L

T

A

T

V

H

G

E

P

I

A

A

A

S

E

A

L

F

L

H

T

A

P

G

G

S

L

H

H

G

G

S

S

T

T

Y

F

G

G

N

N

P

P

L

V

A

C

C

A

A

A

V

A

A

A

N

L

A

A

A

V

F

L

D

R

L

V

I

L

N

A

T

S

P

-

A

-

V

-

L

-

D

D

G

G

V

L

S

V

A

R

K

R

R

A

E

E

L

V

F

L

V

G

K

K

H

S

L

L

Q

R

Q

H

-

G

L

I

D

E

A

A

-

L

E

G

F

H

D

P

L

L

F

I

S

D

D

H

I

V

R

R

G

G

M

R

G

G

L

L

I

L

G

G

A

I

E

A

L

L

K

T

P

A

Q

P

H

H

K

-

G

-

R

-

A

A

R

K

D

D

F

A

L

E

Y

A

A

T

A

A

A

R

Q

D

A

A

G

G

V

Y

M

L

V

V

L

N

N

A

A

A

G

A

P

P

D

D

V

V

M

I

R

R

F

L

V

A

P

P

S

P

L

L

I

I

D

A

E

E

Q

A

D

Q

I

L

active site: F133 (= F141), D218 (= D226), K247 (= K255)
binding 6-methoxyquinoline-3-carboxylic acid: N75 (= N79), L76 (≠ V80)

7nn4A Crystal structure of mycobacterium tuberculosis argd with prosthetic group pyridoxal 5'-phosphate and 3-hydroxy-2-naphthoic acid.
41% identity, 94% coverage: 7:389/406 of query aligns to 3:376/391 of 7nn4A

query
sites
7nn4A

A

A

I

T

T

M

R

R

A

Q

T

R

F

W

D

Q

E

A

V

V

I

M

L

M

P

N

I

N

Y
|
Y

A

G

P

T

A

P

E

P

F

I

I

A

P

L

V

A

K

S

G

G

K

D

G

G

S

A

R

V

V

V

W

T

D

D

Q

V

Q

D

G

G

K

R

E

T

Y

Y

V

I

D

D

F

L

A

L

G

G

I

I

A

A

V

V

T

N

A

V

L

L

G

G

H

H

C

R

H

H

P

P

A

A

L

V

V

I

A

E

A

A

L

V

H

T

E

R

Q

Q

G

M

E

S

T

T

L

L

W

G

H

H

T

T

S

S

N

N

V
x
L

F

Y

T

A

N

T

E

E

P

P

A

G

L

I

R

A

L

L

G

A

C

E

K

E

L

L

V

V

E

A

A

L

T

L

F

G

A

A

E

D

-

Q

-

R

-

T

R

R

V

V

V

F

F

F

M

C

N

N

S

S

G

G

T

A

E

E

A

A

N

N

E

E

T

A

A

A

F

F

K

K

L

L

A

S

R

R

H

L

Y

T

A

G

V

-

T

-

R

-

H

-

S

-

P

-

Y

-

K

R

T

T

K

K

I

L

I

V

A

A

F

A

H

H

N

D

A

A

F
|
F

H

H

G

G

R
|
R

S

T

L

M

F

G

T

S

V

L

S

A

V

L

G

T

G

G

Q

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active site: F133 (= F141), D218 (= D226), K247 (= K255)
binding 3-hydroxynaphthalene-2-carboxylic acid: Y17 (= Y21), L76 (≠ V80), R136 (= R144), G274 (= G282)

Query Sequence

>WP_012967145.1 NCBI__GCF_000025465.1:WP_012967145.1
MATEQPAITRATFDEVILPIYAPAEFIPVKGKGSRVWDQQGKEYVDFAGGIAVTALGHCH
PALVAALHEQGETLWHTSNVFTNEPALRLGCKLVEATFAERVVFMNSGTEANETAFKLAR
HYAVTRHSPYKTKIIAFHNAFHGRSLFTVSVGGQPKYSDGFGPKPADIVHIPFNDLQAVK
AVMDDHTCAVVVEPIQGEGGVTAATPAFLQGLRELCDQHQALLVFDEVQCGMGRTGSLFA
YMHYGVTPDILTSAKALGGGFPVSAMLTTHEIASAFHAGSHGSTYGGNPLACAVANAAFD
LINTPAVLDGVSAKRELFVKHLQQLDAEFDLFSDIRGMGLLIGAELKPQHKGRARDFLYA
AAQAGVMVLNAGPDVMRFVPSLIIDEQDIAEGMARFAQAVAKVING

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory