SitesBLAST
Comparing WP_012968177.1 NCBI__GCF_000025465.1:WP_012968177.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1gl3A Aspartate beta-semialdehyde dehydrogenase in complex with NADP and substrate analogue s-methyl cysteine sulfoxide (see paper)
55% identity, 99% coverage: 1:368/372 of query aligns to 1:366/367 of 1gl3A
- active site: C135 (= C135), Q162 (= Q162), R267 (= R268), H274 (= H275)
- binding cysteine: C135 (= C135), Q162 (= Q162), I230 (= I230), E241 (= E241), R267 (= R268), H274 (= H275)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: R10 (= R10), G11 (= G11), M12 (= M12), V13 (= V13), T37 (= T37), S38 (= S38), C72 (= C72), Q73 (= Q73), A98 (= A98), C135 (= C135), S165 (= S165), G166 (= G166), G168 (= G168), A169 (= A169), Q350 (= Q352), A355 (= A357)
P0A9Q9 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Escherichia coli (strain K12) (see 2 papers)
55% identity, 99% coverage: 1:368/372 of query aligns to 1:366/367 of P0A9Q9
- RGMV 10:13 (= RGMV 10:13) binding NADP(+)
- TS 37:38 (= TS 37:38) binding NADP(+)
- Q73 (= Q73) binding NADP(+)
- C135 (= C135) active site, Acyl-thioester intermediate; mutation to A: Complete loss of activity.; mutation to S: 99.7% loss of activity.
- SGGGA 165:169 (≠ SGAGA 165:169) binding NADP(+)
- R173 (= R173) binding NADP(+)
- P193 (≠ A193) binding NADP(+)
- Q350 (= Q352) binding NADP(+)
5bntC X-ray crystal structure of a aspartate-semialdehyde dehydrogenase bound to NADP from pseudomonas aeruginosa
54% identity, 100% coverage: 1:371/372 of query aligns to 2:371/371 of 5bntC
- active site: C136 (= C135), Q163 (= Q162), R269 (= R268), H276 (= H275)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), R11 (= R10), G12 (= G11), M13 (= M12), V14 (= V13), T38 (= T37), S39 (= S38), Q74 (= Q73)
4r5mA Crystal structure of vc-aspartate beta-semialdehyde-dehydrogenase with NADP and 4-nitro-2-phosphono-benzoic acid (see paper)
51% identity, 99% coverage: 3:371/372 of query aligns to 2:369/369 of 4r5mA
- active site: C134 (= C135), Q161 (= Q162), R267 (= R268), H274 (= H275)
- binding 4-nitro-2-phosphonobenzoic acid: C71 (= C72), G73 (= G74), G74 (= G75), A96 (= A97), A97 (= A98), S98 (= S99), R101 (= R102), K243 (= K244)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), T36 (= T37), S37 (= S38), Q72 (= Q73), G73 (= G74), G165 (= G166)
1mb4A Crystal structure of aspartate semialdehyde dehydrogenase from vibrio cholerae with NADP and s-methyl-l-cysteine sulfoxide (see paper)
51% identity, 99% coverage: 3:371/372 of query aligns to 2:369/369 of 1mb4A
- active site: C134 (= C135), Q161 (= Q162), R267 (= R268), H274 (= H275)
- binding cysteine: N133 (= N134), C134 (= C135), G165 (= G166), E240 (= E241), R267 (= R268), H274 (= H275)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), T36 (= T37), S37 (= S38), C71 (= C72), Q72 (= Q73), G73 (= G74), A97 (= A98), S164 (= S165), Q350 (= Q352), L351 (= L353), A355 (= A357)
3pzrA Crystals structure of aspartate beta-semialdehyde dehydrogenase from vibrio cholerae with NADP and product of s-carbamoyl-l-cysteine (see paper)
51% identity, 99% coverage: 3:371/372 of query aligns to 2:369/370 of 3pzrA
- active site: C134 (= C135), Q161 (= Q162), R267 (= R268), H274 (= H275)
- binding cysteine: C134 (= C135), Q161 (= Q162), G165 (= G166), E240 (= E241), R267 (= R268), H274 (= H275), Q350 (= Q352)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G7 (= G8), R9 (= R10), G10 (= G11), M11 (= M12), V12 (= V13), S35 (= S36), T36 (= T37), S37 (= S38)
Q9KQG2 Aspartate-semialdehyde dehydrogenase 1; ASA dehydrogenase 1; ASADH 1; Aspartate-beta-semialdehyde dehydrogenase 1; EC 1.2.1.11 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961) (see paper)
51% identity, 99% coverage: 3:371/372 of query aligns to 2:369/370 of Q9KQG2
- RGMV 9:12 (= RGMV 10:13) binding NADP(+)
- TS 36:37 (= TS 37:38) binding NADP(+)
- Q72 (= Q73) binding NADP(+)
- C134 (= C135) active site, Acyl-thioester intermediate
- SG 164:165 (= SG 165:166) binding NADP(+)
- P192 (≠ A193) binding NADP(+)
- Q350 (= Q352) binding NADP(+)
P44801 Aspartate-semialdehyde dehydrogenase; ASA dehydrogenase; ASADH; Aspartate-beta-semialdehyde dehydrogenase; EC 1.2.1.11 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see 2 papers)
50% identity, 99% coverage: 1:369/372 of query aligns to 1:370/371 of P44801
- RGMV 10:13 (= RGMV 10:13) binding NADP(+)
- TS 37:38 (= TS 37:38) binding NADP(+)
- Q74 (= Q73) binding NADP(+)
- R103 (= R102) binding phosphate; mutation to K: 2-fold increase in affinity for ASA, 23-fold decrease in affinity for phosphate, and 275-fold decrease in activity.; mutation to L: 7-fold increase in affinity for ASA, 150-fold decrease in affinity for phosphate, and 1400-fold decrease in activity.
- C136 (= C135) active site, Acyl-thioester intermediate
- S166 (= S165) binding NADP(+)
- E243 (= E241) mutation to D: No change in affinity for ASA and 82-fold decrease in activity.
- K246 (= K244) binding phosphate; mutation to R: 2-fold increase in affinity for ASA, nearly no change in affinity for phosphate, and 30-fold decrease in activity.
- R270 (= R268) mutation to K: 2-fold decrease in affinity for ASA and 825-fold decrease in activity.
- H277 (= H275) active site, Proton acceptor
- Q353 (= Q352) binding NADP(+)
1pquA Crystal structure of the h277n mutant of aspartate semialdehyde dehydrogenase from haemophilus influenzae bound with NADP, s-methyl cysteine sulfoxide and cacodylate (see paper)
50% identity, 99% coverage: 1:369/372 of query aligns to 1:370/371 of 1pquA
- active site: C136 (= C135), Q163 (= Q162), R270 (= R268), N277 (≠ H275)
- binding cysteine: C136 (= C135), Q163 (= Q162), G167 (= G166), E243 (= E241), R270 (= R268)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), R10 (= R10), G11 (= G11), M12 (= M12), V13 (= V13), T36 (≠ S36), T37 (= T37), S38 (= S38), C73 (= C72), Q74 (= Q73), A99 (= A98), S166 (= S165), L354 (= L353), A358 (= A357)
7tcmA Crystal structure of aspartate-semialdehyde dehydrogenase from acinetobacter baumannii in complex with NADP
51% identity, 99% coverage: 3:369/372 of query aligns to 4:369/373 of 7tcmA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), R11 (= R10), G12 (= G11), M13 (= M12), V14 (= V13), T38 (= T37), S39 (= S38), C74 (= C72), Q75 (= Q73), G76 (= G74), A100 (= A98), S167 (= S165), Q352 (= Q352), A357 (= A357)
1tb4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound periodate (see paper)
49% identity, 99% coverage: 1:370/372 of query aligns to 1:357/357 of 1tb4A
1ta4A Crystal structure of aspartate-semialdehyde dehydrogenase from haemophilus influenzae with a bound arsenate (see paper)
49% identity, 99% coverage: 1:370/372 of query aligns to 1:357/357 of 1ta4A