SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012968595.1 NCBI__GCF_000025465.1:WP_012968595.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
91% identity, 100% coverage: 1:473/473 of query aligns to 1:472/472 of P78061

query
sites
P78061

M

M

E

E

T

T

N

N

I

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F

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H282 (= H282) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R357) mutation to Q: Activity is impaired to 3% of wild-type.

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
29% identity, 92% coverage: 36:468/473 of query aligns to 16:424/443 of 4lnkA

query
sites
4lnkA

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active site: D52 (≠ P71), E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), H244 (= H282), R315 (= R352), E332 (= E369), R334 (= R371)
binding adenosine-5'-diphosphate: K43 (= K63), M50 (≠ Y69), F198 (≠ L235), Y200 (≠ H237), N246 (≠ H284), S248 (= S286), S324 (≠ G361), S328 (≠ N365), R330 (= R367)
binding glutamic acid: E133 (= E161), E188 (= E225), V189 (≠ A226), N239 (≠ A277), G240 (= G278), G242 (= G280), E303 (≠ Y340)
binding magnesium ion: E131 (= E159), E188 (= E225), E195 (= E232), H244 (= H282), E332 (= E369)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
29% identity, 92% coverage: 36:468/473 of query aligns to 16:424/443 of 4lniA

query
sites
4lniA

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|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

V

A

M

L

A

S

A

V

I

L

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

N

P

K

-

L

-

E

-

A

Q

P

L

A

P

P

L

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

-

S

Q

K

E

E

V

R

E

M

G

E

N

N

G

G

L

I

E

-

Q

-

E

-

G

-

L

V

P

D

F

L

P

P

I

A

R

T

Q

L

S

A

D

E

A

A

L

L

W

E

E

E

F

F

M

K

Q

S

N

N

D

E

H

V

L

M

R

V

E

K

R

A

L

L

G

G

E

E

R

H

F

L

C

-

H

-

V

-

F

-

H

-

A

-

C

-

K

-

H

-

D

-

E

-

L

-

Q

-

F

F

E

E

R

H

L

F

I

I

T

E

E

A

T

K

E

E

I

I

E

E

W

W

active site: D52 (≠ P71), E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), H244 (= H282), R315 (= R352), E332 (= E369), R334 (= R371)
binding adenosine-5'-diphosphate: E131 (= E159), E183 (≠ D220), D197 (≠ N234), Y200 (≠ H237), N246 (≠ H284), S248 (= S286), R320 (= R357), R330 (= R367)
binding magnesium ion: E131 (= E159), E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), E195 (= E232), H244 (= H282), E332 (= E369)
binding l-methionine-s-sulfoximine phosphate: E133 (= E161), E188 (= E225), H244 (= H282), R297 (= R334), E303 (≠ Y340), R315 (= R352), R334 (= R371)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
29% identity, 92% coverage: 36:468/473 of query aligns to 17:425/444 of P12425

query
sites
P12425

D

N

T

V

Q

K

Y

Y

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

G

K

K

-

A

L

L

E

D

K

N

G

K

C

V

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G
|
G

N

F

V

V

-
x
R

V

I

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

Y

G

L

E

Y

P

P

D

D

R

L

S

N

C

T

I

F

P

-

V

-

P

-

G

-

T

V

L

I

T

F

P

P

S

W

A

T

A

A

D

E

P

K

Q

G

S

K

I

V

A

A

Q

R

V

F

Q

I

L

C

T

D

M

I

V

Y

D

N

E

P

D

D

G

G

A

T

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

N

V

N

L

L

N

K

R

R

L

I

W

L

Q

K

Q

E

L

M

R

E

Q

D

R

L

G

G

L

F

F

S

P

D

V

F

-

N

V

L

A

G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

R

L

K

D

R

E

D

K

A

G

E

E

G

P

Y

T

L

L

Q

E

P

-

C

-

A

-

P

-

G

-

T

L

D

N

D

D

R

K

N

G

T

G

Q

Y

S

F

Q

D

V

L

Y

A

S

P

V

T

D

D

-

L

N

G

L

E

N

N

H

C

F

R

A

R

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D

E

G

A

A

S

V

H

A

H

E
|
E

A
x
V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

A

D

G

N

A

V

V

L

-

D

R

A

S

C

C

D

D

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A

N

G
|
G

S

S

G

G

M

M

H
|
H

I

C

H

N

I

L

S

S

M

L

L

F

N

K

N

N

K

-

G

G

E

V

N

N

V

A

L

F

V

F

D

D

-

E

-

N

G

A

D

D

G

L

E

Q

D

L

S

S

A

E

L

T

L

A

K

K

R

H

A

F

L

I

A

A

G

G

M

I

I

V

D

K

L

H

M

A

P

T

A

S

S

F

M

T

A

A

L

V

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

V

P

P

G
|
G

M

Y

Y
x
E

V

A

P
|
P

T

C

Q

Y

A

V

S

A

W

W

G

S

H

A

N

Q

N

N

R

R

T

S

V

P

A

L

L

I

R

R

I

I

P

P

C

A

G

S

E

R

R

G

Q

I

N

S

H

T

R

R

V

V

E
|
E

Y

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

V

A

M

L

A

S

A

V

I

L

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

N

P

K

-

L

-

E

-

A

Q

P

L

A

P

P

L

I

-

D

-

R

-

N

-

I

-

Y

-

V

-

M

-

S

Q

K

E

E

V

R

E

M

G

E

N

N

G

G

L

I

E

-

Q

-

E

-

G

-

L

V

P

D

F

L

P

P

I

A

R

T

Q

L

S

A

D

E

A

A

L

L

W

E

E

E

F

F

M

K

Q

S

N

N

D

E

H

V

L

M

R

V

E

K

R

A

L

L

G

G

E

E

R

H

F

L

C

-

H

-

V

-

F

-

H

-

A

-

C

-

K

-

H

-

D

-

E

-

L

-

Q

-

F

F

E

E

R

H

L

F

I

I

T

E

E

A

T

K

E

E

I

I

E
|
E

W

W

G59 (= G81) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (vs. gap) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E159) binding Mg(2+)
E134 (= E161) binding Mg(2+)
E189 (= E225) binding Mg(2+)
V190 (≠ A226) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E232) binding Mg(2+)
G241 (= G278) binding L-glutamate
H245 (= H282) binding Mg(2+)
G302 (= G338) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ Y340) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P342) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E369) binding Mg(2+)
E424 (= E467) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
29% identity, 92% coverage: 36:468/473 of query aligns to 20:428/447 of 4s0rD

query
sites
4s0rD

D

N

T

V

Q

K

Y

Y

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

G

K

K

-

A

L

L

E

D

K

N

G

K

C

V

Y

M

F

F

P
x
D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N
x
F

V
|
V

-
x
R

V
x
I

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

Y

G

L

E

Y

P

P

D

D

R

L

S

N

C

T

I

F

P

-

V

-

P

-

G

-

T

V

L

I

T

F

P

P

S

W

A

T

A

A

D

E

P

K

Q

G

S

K

I

V

A

A

Q

R

V

F

Q

I

L

C

T

D

M

I

V

Y

D

N

E

P

D

D

G

G

A

T

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

N

V

N

L

L

N

K

R

R

L

I

W

L

Q

K

Q

E

L

M

R

E

Q

D

R

L

G

G

L

F

F

S

P

D

V

F

-

N

V

L

A

G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

K

R

L

K

D

R

E

D

K

A

G

E

E

G

P

Y

T

L

L

Q

E

P

-

C

-

A

-

P

-

G

-

T

L

D

N

D

D

R

K

N

G

T

G

Q

Y

S

F

Q
x
D

V

L

Y

A

S

P

V

T

D

D

-

L

N

G

L

E

N

N

H

C

F

R

A

R

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D

E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

A

D

G

N

A

V

V

L

-

D

R

A

S

C

C

D

D

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E
x
G

H
x
V

A
x
N

G

G

S

S

G

G

M

M

H
|
H

I

C

H

N

I

L

S

S

M

L

L

F

N

K

N

N

K

-

G

G

E

V

N

N

V

A

L

F

V

F

D

D

-

E

-

N

G

A

D

D

G

L

E

Q

D

L

S

S

A

E

L

T

L

A

K

K

R

H

A

F

L

I

A

A

G

G

M

I

I

V

D

K

L

H

M

A

P

T

A

S

S

F

M

T

A

A

L

V

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

V

P

P

G
|
G

M
x
Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

V

S

A

W

W

G

S

H

A

N

Q

N

N

R
|
R

T

S

V

P

A

L

L

I

R

R

I

I

P

P

C

A

G

S

E

R

R

G

Q

I

N

S

H

T

R

R

V

V

E
|
E

Y

V

R
|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

V

A

M

L

A

S

A

V

I

L

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

N

P

K

-

L

-

E

-

A

Q

P

L

A

P

P

L

I

-

D

-

R

-

N

-

I

-
x
Y

-

V

-

M

-

S

Q

K

E

E

V

R

E

M

G

E

N

N

G

G

L

I

E

-

Q

-

E

-

G

-

L

V

P

D

F

L

P

P

I

A

R

T

Q

L

S

A

D

E

A

A

L

L

W

E

E

E

F

F

M

K

Q

S

N

N

D

E

H

V

L

M

R

V

E

K

R

A

L

L

G

G

E

E

R

H

F

L

C

-

H

-

V

-

F

-

H

-

A

-

C

-

K

-

H

-

D

-

E

-

L

-

Q

-

F

F

E

E

R
x
H

L

F

I

I

T

E

E

A

T

K

E

E

I
|
I

E

E

W

W

active site: D56 (≠ P71), E135 (= E159), E137 (= E161), E192 (= E225), E199 (= E232), H248 (= H282), R319 (= R352), E336 (= E369), R338 (= R371)
binding glutamine: E137 (= E161), E192 (= E225), R301 (= R334), E307 (≠ Y340)
binding magnesium ion: I66 (≠ V84), E135 (= E159), E135 (= E159), E199 (= E232), H248 (= H282), H248 (= H282), E336 (= E369), H419 (≠ R459)
binding : F63 (≠ N82), V64 (= V83), R65 (vs. gap), I66 (≠ V84), D161 (≠ Q191), G241 (≠ E275), V242 (≠ H276), N243 (≠ A277), G305 (= G338), Y306 (≠ M339), Y376 (vs. gap), I426 (= I466)

Sites not aligning to the query:

binding : 430

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
29% identity, 95% coverage: 24:471/473 of query aligns to 1:441/443 of 7tf9S

query
sites
7tf9S

A

A

K

K

E

Y

V

T

K

K

R

E

Y

D

L

I

E

F

T

R

Y

F

P

A

D

D

T

E

Q

Q

-

N

-

V

-

K

Y

F

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

I

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

K

K

K

-

A

L

L

E

D

K

N

G

K

C

I

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N
x
F

V
|
V

V

R

E

I

E

E

A

-

G

-

L

-

G

-

Q

-

D

-

M

-

G

-

E

E

P

S

D

D

R

M

S

Y

C

L

I

F

P

P

V

D

P

L

G

D

T

T

-

W

-

V

L

V

T

F

P

P

S

W

A

T

A

A

D

E

P

K

Q

G

S

K

I

V

A

A

Q

R

V

M

Q

I

L

C

T

D

M

I

V

Y

D

N

E

P

D

D

G

M

A

T

P

P

F

F

D

A

V

G

E

D

P

P

R

R

N

A

V

N

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

M

R

E

Q

E

R

L

G

G

L

F

F

T

P

E

V

F

-

N

V

L

A

G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

L

L

D

D

R

E

K

N

R

R

-

R

-

P

-

T

-

L

-

E

-

L

-

N

D

D

A

S

E

G

G

G

Y
|
Y

L

F

Q

D

P

-

C

L

A

A

P

P

G

T

T

D

D

L

D

G

R

E

N

N

T

C

Q

R

S

R

Q

-

V

-

Y

-

S

-

V

-

D

-

N

-

L

-

N

-

H

-

F

-

A

-

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D

E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

E

D

D

N

-

V

A

L

I

D

T

A

A

C

C

D

D

D

S

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A

N

G
|
G

S

S

G
|
G

M

M

H
|
H

I

F

H

N

I

M

S

S

M

L

L

F

N

N

N

E

K

K

G

G

E

-

N

N

V

A

L

F

V

F

D

D

-

E

-

S

G

G

D

E

G

L

E

E

D

L

S

S

A

Q

L

T

L

A

K

Y

R

H

A

F

L

L

A

A

G

G

M

M

I

L

D

K

L

H

M

A

P

R

A

G

S

Y

M

T

A

A

L

V

L

T

A

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

F

L

Q

V

P

P

G

G

M

Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

I

S

A

W

W

G

S

H

G

N

K

N

N

R

R

T

S

V

P

A

L

L

V

R

R

I

V

P

P

C

S

G

S

E

R

R

G

Q

L

N

S

H

T

R

R

V

L

E
|
E

Y

L

R

R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

V

A

M

M

A

A

A

V

I

L

F

L

A

K

G

A

I

G

L

L

H

S

G

G

L

I

D

K

N

D

-

E

-

L

-

T

P

P

Q

P

L

A

P

P

L

V

Q

D

E

R

E

N

V

I

E

Y

G

G

-

M

N

N

G

E

L

E

E

E

Q

R

E

E

G

A

-

T

-

G

-

I

L

Y

P

D

F

L

P

P

I

E

R

S

Q

L

S

G

D

H

A

A

L

L

W

I

E

E

F

L

M

E

Q

K

N

N

D

E

H

I

L

I

R

K

E

D

R

G

L

L

G

G

E

E

R

H

F

I

C

F

H

E

V

H

F

F

H

I

A
x
E

C

A

K

K

H

T

D
x
I

E

E

L

C

L

D

Q
x
M

F

F

E

R

R

T

L

A

I

V

T

H

E

P

T

W

E

E

I

R

E

E

W

Q

M

Y

L

L

K

E

binding glutamine: E133 (= E161), Y155 (= Y174), E188 (= E225), G240 (= G278), G242 (= G280), R297 (= R334), E303 (≠ Y340)
binding magnesium ion: E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), H244 (= H282), E332 (= E369)
binding : F59 (≠ N82), V60 (= V83), E418 (≠ A448), I422 (≠ D452), M426 (≠ Q456)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
28% identity, 92% coverage: 38:471/473 of query aligns to 21:446/447 of 8oooA

query
sites
8oooA

Q

Q

Y

F

V

V

D

D

V

I

L

L

L

G

T

V

D

P

L

K

N

N

G

V

C
x
A

F

F

R

-

G

-

K

-

R

-

I

-

P

P

V

I

A

K

G

A

L

G

S

E

K

K

-

G

-

I

-

E

-

L

-

R

-

D

-

V

L

L

E

E

K

N

G

G

C

L

Y

Y

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

F

V

V

-

G

V

I

E

N

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

M

G

L

E

K

P

P

D

D

R

L

S

S

C

T

I

F

P

-

V

-

P

-

G

-

T

S

L

V

T

L

P

P

S

W

A
x
R

A

P

D

S

P

E

Q

K

S

S

I
x
V

A

A

Q

R

V

V

Q

I

L

C

T

D

M

V

V

Y

D

T

E

T

D

K

G

G

A

K

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

G

V

C

L

L

N

K

R

R

L

V

W

M

Q

E

L

F

R

K

Q

K

R

E

-

F

-

N

G

G

L

E

F

Y

P

-

V

F

V

V

A

G

V

P

E
|
E

L

P

E

E

F

F

Y

F

L

L

D

-

R

-

K

K

R

K

D

D

A

-

E

-

G

-

Y

-

L

-

Q

-

P

-

P

P

C

H

A

N

P

P

G

H

T

K

D

Y

D

I

R

P

N

A

T

D

Q

D

S

G

Q

G

V

Y

Y

F

S

D

V

L

D

E

N

P

L

M

N

D

H

E

F

A

A
x
P

D

D

V

I

L
x
R

Y
x
R

D

D

I

I

D

V

E

F

L

A

A

L

K

E

L

N

Q

L

L

G

I

F

P

H

A

V

D
x
E

G

A

A
x
S

V

H

A

H

E

E

A

V

S

A

P

P

G

G

Q

Q

F

H

E

E

I

V

N

D

L
x
F

H
x
K

H
x
F

T

-

D

D

N

D

V

A

L

L

D

K

A

T

C

A

D

D

D

S

A

V

L

I

A

T

L

F

K

K

R

T

L

T

V

I

R

K

L

T

M

I

A

A

E

E

K

Q

H

H

K

G

M

L

H

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

E

F

E

G

H

M

A

N

G

G

S

S

G

G

M

M

H

H

I

C

H
|
H

I

Q

S
|
S

M

I

L

W

N

L

N

N

K

-

G

G

E

E

N

P

V

S

L

F

V

Y

D

D

G

E

D

N

G

A

-

P

-

Y

E

Q

D

L

S

S

A

E

L

T

L

C

K

M

R

N

A

Y

L

V

A

A

G

G

M

I

I

L

D

K

L

H

M

A

P

K

A

A

S

I

M

V

A

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

V

P

P

G

G

M

Y

Y

E

V

A

P

P

T

V

Q

N

A

I

S

A

W

W

G

A

H

N

N

S

N

N

R

R

T

S

V

A

A

I

L

I

R
|
R

I

V

P

P

C

A

G

A

E

R

R

G

Q

K

N

G

H

T

R
|
R

V

I

E

E

Y

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

V

A

M

F

A

T

A

V

I

M

F

L

A

A

-

A

-

G

-

L

-

D

G

G

I

V

L

K

H

N

G

K

L

L

D

D

N

A

P

P

Q

E

L

-

P

P

L

V

Q

E

-

R

-

N

-

I

-

F

-

A

-

M

E

S

E

E

V

A

E

E

G

K

N

K

G

E

L

L

E

G

Q

I

E

E

G

S

L

V

P

P

F

A

P

N

I

L

R

K

Q

A

S

-

D

-

A

A

L

L

W

D

E

E

F

L

M

E

Q

N

N

N

D

D

H

V

L

L

R

K

E

N

R

A

L

L

G

G

E

K

R

H

F

I

C

F

H

E

V

S

F

F

H

L

A

E

C

I

K

K

H

N

D

A

E

E

L

W

L

D

Q

S

F

F

E

R

R

T

L

S

I

V

T

T

E

D

T

W

E

E

I

T

E

T

W

A

M

Y

L

L

K

K

binding 2-oxoglutaric acid: A33 (≠ C50), R87 (≠ A111), V93 (≠ I117), P170 (≠ A202), R173 (≠ L205), R174 (≠ Y206), S190 (≠ A222)
binding adenosine-5'-triphosphate: E136 (= E159), E188 (≠ D220), F203 (≠ L235), K204 (≠ H236), F205 (≠ H237), H251 (= H284), S253 (= S286), R325 (= R357), R335 (= R367)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 17, 19

7tenA Glutamine synthetase (see paper)
29% identity, 92% coverage: 36:471/473 of query aligns to 15:440/442 of 7tenA

query
sites
7tenA

D

N

T

V

Q

K

Y

F

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

I

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

K

K

K

-

A

L

L

E

D

K

N

G

K

C

I

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

F

V

V

V

R

E

I

E

E

A

-

G

-

L

-

G

-

Q

-

D

-

M

-

G

-

E

E

P

S

D

D

R

M

S

Y

C

L

I

F

P

P

V

D

P

L

G

D

T

T

-

W

-

V

L

V

T

F

P

P

S

W

A

T

A

A

D

E

P

K

Q

G

S

K

I

V

A

A

Q

R

V

M

Q

I

L

C

T

D

M

I

V

Y

D

N

E

P

D

D

G

M

A

T

P

P

F

F

D

A

V

G

E

D

P

P

R

R

N

A

V

N

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

M

R

E

Q

E

R

L

G

G

L

F

F

T

P

E

V

F

-

N

V

L

A
x
G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

-

F

-

K

L

L

D

D

R

E

K

N

R

R

-

R

-

P

-

T

-

L

-

E

-

L

-

N

D

D

A

S

E

G

G

G

Y

Y

L

F

Q

D

P

-

C

L

A

A

P

P

G

T

T

D

D

L

D

G

R

E

N

N

T

C

Q

R

S

R

Q

-

V

-

Y

-

S

-

V

-

D

-

N

-

L

-

N

-

H

-

F

-

A

-

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D
x
E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N
x
D

L
x
F

H
x
K

H
x
Y

T

E

D

D

N

-

V

A

L

I

D

T

A

A

C

C

D

D

D

S

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A
x
N

G
|
G

S

S

G

G

M

M

H
|
H

I

F

H
x
N

I

M

S
|
S

M

L

L

F

N

N

N

E

K

K

G

G

E

-

N

N

V

A

L

F

V

F

D

D

-

E

-

S

G

G

D

E

G

L

E

E

D

L

S

S

A

Q

L

T

L

A

K

Y

R

H

A

F

L

L

A

A

G

G

M

M

I

L

D

K

L

H

M

A

P

R

A

G

S

Y

M

T

A

A

L

V

L

T

A

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

F

L

Q

V

P

P

G

G

M

Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

I

S

A

W

W

G

S

H

G

N

K

N

N

R
|
R

T

S

V

P

A

L

L

V

R
|
R

I

V

P

P

C

S

G

S

E

R

R

G

Q

L

N
x
S

H

T

R
|
R

V

L

E

E

Y

L

R
|
R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

V

A

M

M

A

A

A

V

I

L

F

L

A

K

G

A

I

G

L

L

H

S

G

G

L

I

D

K

N

D

-

E

-

L

-

T

P

P

Q

P

L

A

P

P

L

V

Q

D

E

R

E

N

V

I

E

Y

G

G

-

M

N

N

G

E

L

E

E

E

Q

R

E

E

G

A

-

T

-

G

-

I

L

Y

P

D

F

L

P

P

I

E

R

S

Q

L

S

G

D

H

A

A

L

L

W

I

E

E

F

L

M

E

Q

K

N

N

D

E

H

I

L

I

R

K

E

D

R

G

L

L

G

G

E

E

R

H

F

I

C

F

H

E

V

H

F

F

H

I

A

E

C

A

K

K

H

T

D

I

E

E

L

C

L

D

Q

M

F

F

E

R

R

T

L

A

I

V

T

H

E

P

T

W

E

E

I

R

E

E

W

Q

M

Y

L

L

K

E

binding adenosine-5'-diphosphate: G128 (≠ A157), E130 (= E159), E182 (≠ D220), D196 (≠ N234), F197 (≠ L235), K198 (≠ H236), Y199 (≠ H237), N245 (≠ H284), S247 (= S286), R319 (= R357), S327 (≠ N365), R329 (= R367)
binding l-methionine-s-sulfoximine phosphate: E130 (= E159), E132 (= E161), E187 (= E225), E194 (= E232), N238 (≠ A277), G239 (= G278), H243 (= H282), R296 (= R334), E302 (≠ Y340), R314 (= R352), R333 (= R371)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
28% identity, 92% coverage: 38:471/473 of query aligns to 20:445/446 of 8ooqB

query
sites
8ooqB

Q

Q

Y

F

V

V

D

D

V

I

L

L

L

G

T

V

D

P

L

K

N

N

G

V

C
x
A

F

F

R

-

G

-

K

-

R

-

I

-

P

P

V

I

A

K

G

A

L

G

S

E

K

K

-

G

-

I

-

E

-

L

-

R

-

D

-

V

L

L

E

E

K

N

G

G

C

L

Y

Y

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

F

V

V

-

G

V

I

E

N

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

M

G

L

E

K

P

P

D

D

R

L

S

S

C

T

I

F

P

-

V

-

P

-

G

-

T

S

L

V

T

L

P

P

S

W

A
x
R

A

P

D

S

P

E

Q

K

S

S

I
x
V

A

A

Q

R

V

V

Q

I

L

C

T

D

M

V

V

Y

D

T

E

T

D

K

G

G

A

K

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

G

V

C

L

L

N

K

R

R

L

V

W

M

Q

E

L

F

R

K

Q

K

R

E

-

F

-

N

G

G

L

E

F

Y

P

-

V

F

V

V

A

G

V

P

E

E

L

P

E
|
E

F

F

Y

F

L

L

D

-

R

-

K

K

R

K

D

D

A

-

E

-

G

-

Y

-

L

-

Q

-

P

-

P

P

C

H

A

N

P

P

G

H

T

K

D

Y

D

I

R

P

N

A

T

D

Q

D

S

G

Q

G

V

Y

Y

F

S

D

V

L

D

E

N

P

L

M

N

D

H

E

F

A

A
x
P

D

D

V

I

L
x
R

Y
x
R

D

D

I

I

D

V

E

F

L

A

A

L

K

E

L

N

Q

L

L

G

I

F

P

H

A

V

D

E

G

A

A
x
S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

V

N

D

L

F

H

K

H

F

T

-

D

D

N

D

V

A

L

L

D

K

A

T

C

A

D

D

D

S

A

V

L

I

A

T

L

F

K

K

R

T

L

T

V

I

R

K

L

T

M

I

A

A

E

E

K

Q

H

H

K

G

M

L

H

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

E

F

E

G

H

M

A

N

G

G

S

S

G

G

M

M

H

H

I

C

H

H

I

Q

S

S

M

I

L

W

N

L

N

N

K

-

G

G

E

E

N

P

V

S

L

F

V

Y

D

D

G

E

D

N

G

A

-

P

-

Y

E

Q

D

L

S

S

A

E

L

T

L

C

K

M

R

N

A

Y

L

V

A

A

G

G

M

I

I

L

D

K

L

H

M

A

P

K

A

A

S

I

M

V

A

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

V

P

P

G

G

M

Y

Y

E

V

A

P

P

T

V

Q

N

A

I

S

A

W

W

G

A

H

N

N

S

N

N

R

R

T

S

V

A

A

I

L

I

R

R

I

V

P

P

C

A

G

A

E

R

R

G

Q

K

N

G

H

T

R

R

V

I

E

E

Y

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

V

A

M

F

A

T

A

V

I

M

F

L

A

A

-

A

-

G

-

L

-

D

G

G

I

V

L

K

H

N

G

K

L

L

D

D

N

A

P

P

Q

E

L

-

P

P

L

V

Q

E

-

R

-

N

-

I

-

F

-

A

-

M

E

S

E

E

V

A

E

E

G

K

N

K

G

E

L

L

E

G

Q

I

E

E

G

S

L

V

P

P

F

A

P

N

I

L

R

K

Q

A

S

-

D

-

A

A

L

L

W

D

E

E

F

L

M

E

Q

N

N

N

D

D

H

V

L

L

R

K

E

N

R

A

L

L

G

G

E

K

R

H

F

I

C

F

H

E

V

S

F

F

H

L

A

E

C

I

K

K

H

N

D

A

E

E

L

W

L

D

Q

S

F

F

E

R

R

T

L

S

I

V

T

T

E

D

T

W

E

E

I

T

E

T

W

A

M

Y

L

L

K

K

binding 2-oxoglutaric acid: A32 (≠ C50), R86 (≠ A111), V92 (≠ I117), P169 (≠ A202), R172 (≠ L205), R173 (≠ Y206), S189 (≠ A222)
binding magnesium ion: E137 (= E161), E192 (= E225), E199 (= E232)

Sites not aligning to the query:

binding 2-oxoglutaric acid: 16, 18

7tdvC Glutamine synthetase (see paper)
28% identity, 95% coverage: 22:471/473 of query aligns to 2:441/443 of 7tdvC

query
sites
7tdvC

A

T

F

F

A

T

K

K

E

E

V

D

K

I

R

R

Y

K

L

F

E

V

T

E

Y

E

P

E

D

N

T

V

Q

R

Y

Y

V

L

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

V

P

P

V

V

A

S

G

Q

L

L

S

E

K

K

-

V

L

L

E

D

K

N

G

E

C

M

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

F

V

V

-

R

V

I

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

Y

G

L

E

H

P

P

D

D

R

L

S

D

C

T

I

-

P

-

V

-

P

-

G

W

T

V

L

I

T

F

P

P

S

W

A

T

A

A

D

G

P

Q

Q

G

S

K

I

V

A

A

Q

R

V

L

Q

I

L

C

T

D

M

V

V

Y

D

K

E

T

D

D

G

G

A

T

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

A

V

N

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

M

R

E

Q

D

R

L

G

G

L

F

F

T

P

D

V

F

-

N

V

L

A
x
G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

-

R

-

K

K

R

L

D

D

A

E

E

K

G

G

Y

-

L

-

Q

-

P

E

P

P

C

T

A

L

P

E

G

L

T

N

D

D

R

G

N

G

T

Y

Q

F

S

D

Q

L

V

A

Y

P

S

T

V

D

D

L

N

G

L

E

N

N

H

C

F

R

A

R

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

D

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

D

A

I

D
x
E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N
x
D

L
x
F

H
x
K

H
x
Y

T

A

D

D

N

A

V

V

L

T

D

-

A

A

C

C

D

D

D

N

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

N

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A

N

G
|
G

S

S

G

G

M

M

H
|
H

I

F

H
x
N

I
x
V

S
|
S

M

L

L

F

N

K

N

G

K

K

G

-

E

E

N

N

V

A

L

F

V

F

D

D

G

P

D

N

G

T

E

E

D

-

S

M

A

G

L

L

L

T

K

E

R

T

A

A

L

Y

-

Q

-

F

-

T

A

A

G

G

M

V

I

L

D

K

L

N

M

A

P

R

A

G

S

F

M

T

A

A

L

V

L

C

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

V

P

P

G

G

M

Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

I

S

A

W

W

G

S

H

G

N

K

N

N

R
|
R

T

S

V

P

A

L

L

I

R
|
R

I

V

P

P

C

S

G

S

E

R

R

G

Q

L

N
x
S

H

T

R
|
R

V

I

E
|
E

Y

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

V

A

M

L

A

A

I

I

F

L

A

E

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

N

-

K

-

L

-

K

-

V

P

P

Q

E

L

P

P

V

L

N

Q

Q

E

N

E

I

V

Y

E

E

G

M

N

N

G

R

L

E

E

E

Q

R

E

E

G

A

L

V

-

G

-

I

-

Q

P

D

F

L

P

P

I

S

R

T

Q

L

S

Y

D

T

A

A

L

L

W

K

E

A

F

M

M

R

Q

E

N

N

D

E

H

V

L

I

R

K

E

K

R

A

L

L

G

G

E

N

R

H

F

I

C

Y

H

N

V

Q

F

F

H

I

A

N

C

S

K

K

H

S

D

I

E

E

L

W

L

D

Q

Y

F

Y

E

R

R

T

L

Q

I

V

T

S

E

E

T

W

E

E

I

R

E

D

W

Q

M

Y

L

M

K

K

binding adenosine-5'-diphosphate: G129 (≠ A157), E131 (= E159), E183 (≠ D220), D197 (≠ N234), F198 (≠ L235), K199 (≠ H236), Y200 (≠ H237), N246 (≠ H284), V247 (≠ I285), S248 (= S286), R320 (= R357), S328 (≠ N365), R330 (= R367)
binding magnesium ion: E131 (= E159), E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), E195 (= E232), H244 (= H282), E332 (= E369)
binding l-methionine-s-sulfoximine phosphate: E131 (= E159), E133 (= E161), E188 (= E225), E195 (= E232), G240 (= G278), H244 (= H282), R297 (= R334), E303 (≠ Y340), R315 (= R352)

7tf6A Glutamine synthetase (see paper)
28% identity, 95% coverage: 22:471/473 of query aligns to 1:436/438 of 7tf6A

query
sites
7tf6A

A

T

F

F

A

T

K

K

E

E

V

D

K

I

R

R

Y

K

L

F

E

A

T

E

Y

E

P

E

D

N

T

V

Q

R

Y

Y

V

L

D

R

V

L

L

Q

L

F

T

T

D

D

L

I

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

V

P

P

V

V

A

S

G

Q

L

L

S

E

K

K

-

V

L

L

E

D

K

N

G

E

C

M

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N
x
F

V

V

-
x
R

V

I

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

Y

G

L

E

H

P

P

D

D

R

L

S

D

C

T

I

-

P

-

V

-

P

-

G

W

T

V

L

I

T

F

P

P

S

W

A

G

A

K

D

V

P

A

Q

R

S

L

I

I

A

C

Q

D

V

V

Q

Y

L

K

T

T

M

-

V

-

D

-

E

-

D

D

G

G

A

T

P

P

F

F

D

E

V

G

E

D

P

P

R

R

N

A

V

N

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

M

R

E

Q

D

R

L

G

G

L

F

F

T

P

D

V

F

-

N

V

L

A

G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

-

R

-

K

K

R

L

D

D

A

E

E

K

G

G

Y

-

L

-

Q

-

P

E

P

P

C

T

A

L

P

E

G

L

T

N

D

D

R

G

N

G

T

Y

Q

F

S

D

Q

L

V

A

Y

P

S

T

V

D

D

L

N

G

L

E

N

N

H

C

F

R

A

R

D

D

V

I

L

V

Y

L

D

E

I

L

D

E

E

D

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

D

A

I

D

E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

A

D

D

N

A

V

V

L

T

D

-

A

A

C

C

D

D

D

N

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

K

H

H

K

N

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E
x
G

H

V

A
x
N

G
|
G

S

S

G

G

M

M

H
|
H

I

F

H

N

I

V

S

S

M

L

L

F

N

K

N

G

K

K

G

-

E

E

N

N

V

A

L

F

V

F

D

D

G

P

D

N

G

T

E

E

D

-

S

M

A

G

L

L

L

T

K

E

R

T

A

A

L

Y

-

Q

-

F

-

T

A

A

G

G

M

V

I

L

D

K

L

N

M

A

P

R

A

G

S

F

M

T

A

A

L

V

L

C

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

V

P

P

G
|
G

M
x
Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

I

S

A

W

W

G

S

H

G

N

K

N

N

R
|
R

T

S

V

P

A

L

L

I

R

R

I

V

P

P

C

S

G

S

E

R

R

G

Q

L

N

S

H

T

R

R

V

I

E
|
E

Y

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

V

A

M

L

A

A

I

I

F

L

A

E

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

N

-

K

-

L

-

K

-

V

P

P

Q

E

L

P

P

V

L

N

Q

Q

E

N

E

I

V
x
Y

E

E

G

M

N

N

G

R

L

E

E

E

Q

R

E

E

G

A

L

V

-

G

-

I

-

Q

P

D

F

L

P

P

I

S

R

T

Q

L

S

Y

D

T

A

A

L

L

W

K

E

A

F

M

M

R

Q

E

N

N

D

E

H

V

L

I

R

K

E

K

R

A

L

L

G

G

E

N

R

H

F

I

C

Y

H

N

V

Q

F

F

H

I

A

N

C

S

K

K

H

S

D

I

E

E

L

W

L

D

Q
x
Y

F

Y

E

R

R

T

L

Q

I

V

T

S

E

E

T

W

E

E

I

R

E

D

W
x
Q

M

Y

L

M

K

K

binding glutamine: E128 (= E161), E183 (= E225), G235 (= G278), H239 (= H282), R292 (= R334), E298 (≠ Y340)
binding magnesium ion: E126 (= E159), E128 (= E161), E183 (= E225), E190 (= E232), H239 (= H282), E327 (= E369)
binding : F58 (≠ N82), R60 (vs. gap), G232 (≠ E275), N234 (≠ A277), G296 (= G338), Y297 (≠ M339), R310 (= R352), Y367 (≠ V405), Y421 (≠ Q456), Q433 (≠ W468)

Sites not aligning to the query:

binding : 437

7tfaB Glutamine synthetase (see paper)
28% identity, 88% coverage: 36:453/473 of query aligns to 15:421/441 of 7tfaB

query
sites
7tfaB

D

N

T

V

Q

R

Y

F

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

L

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

E

K

K

-

A

L

L

E

D

K

N

G

K

C

M

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N
x
Y

V

V

-
x
R

V

I

E

E

A

S

G

D

L

M

G

-

Q

-

D

-

M

Y

G

L

E

Y

P

P

D

D

R

L

S

D

C

T

I

W

P

V

V

V

P

F

G

P

T

W

L

V

T

T

P

S

S

D

A

-

D

-

P

-

Q

-

S

R

I

V

A

A

Q

R

V

L

Q

I

L

C

T

D

M

I

V

Y

D

K

E

P

D

D

G

G

A

S

P

P

F

F

D

A

V

G

E

D

P

P

R

R

N

G

V

I

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

A

R

E

Q

E

R

L

G

G

L

Y

F

T

P

S

V

M

-

N

V

V

A

G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

-

R

-

K

K

R

T

D

D

A

E

E

K

G

G

Y

-

L

-

Q

-

P

-

C

-

A

-

P

D

G

P

T

T

D

T

D

E

R

L

N

N

T

D

Q

Q

S

G

Q

G

V
x
Y

Y

F

S

D

V

L

D

A

N

P

L

M

-

D

-

L

-

G

-

E

N

N

H

C

F

R

A

R

D

E

V

I

L

V

Y

L

D

K

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D

E

G

A

A

S

V

H

A

H

E
|
E

A
x
V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

A

D

D

N

A

V

V

L

-

D

K

A

A

C

A

D

D

D

Q

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

Q

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A
x
N

G
|
G

S

S

G

G

M

M

H
|
H

I

C

H

N

I

Q

S

S

M

L

L

F

N

K

N

D

K

-

G

N

E

E

N

N

V

V

L

F

V

Y

D

D

G

E

D

T

G

D

E

E

D

L

-

G

-

L

S

S

A

Q

L

T

L

A

K

R

R

H

A

Y

L

M

A

A

G

G

M

I

I

L

D

K

L

H

M

A

P

R

A

A

S

M

M

A

A

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

V

P

P

G
|
G

M
x
Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

V

S

A

W

W

G

S

H

A

N

S

N

N

R
|
R

T

S

V

P

A

M

L

I

R

R

I

I

P

P

C

A

G

S

E

R

R

G

Q

L

N

S

H

T

R

R

V

V

E
|
E

Y

V

R

R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

V

A

M

L

A

A

A

V

I

M

F

L

A

R

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

R

P

-

Q

Q

L

M

P

A

L

L

Q

P

E

A

E

P

V

I

E

D

G

R

N

N

G

I

L

Y

E

V

-

M

-

S

-

E

-

R

-

I

Q

E

E

E

G

G

L

I

P

P

-

S

F

L

P

P

I

A

R

D

Q

L

S

K

D

E

A

A

L

L

W

S

E

E

F

L

M

I

Q

R

N

S

D

E

H

V

L

I

R

S

E

D

R

A

L

L

G

G

E

D

R

H

F

A

C

L

H

A

V

Y

F

F

H

Y

A

E

C

L

K

K

H

E

D

I

E

E

binding glutamine: E131 (= E161), Y153 (≠ V192), E186 (= E225), G238 (= G278), H242 (= H282), R295 (= R334), E301 (≠ Y340)
binding magnesium ion: E129 (= E159), E131 (= E161), E186 (= E225), E193 (= E232), H242 (= H282), E330 (= E369)
binding : Y58 (≠ N82), R60 (vs. gap), V187 (≠ A226), N237 (≠ A277), G299 (= G338), Y300 (≠ M339), R313 (= R352)

Sites not aligning to the query:

binding : 424

8oozA Glutamine synthetase (see paper)
30% identity, 81% coverage: 26:408/473 of query aligns to 5:357/430 of 8oozA

query
sites
8oozA

E

E

V

I

K

F

R

R

Y

I

L

V

E

E

T

E

Y

-

P

K

D

N

T

V

Q

R

Y

F

V

V

D

R

V

L

L

Q

L

F

T

V

D

D

L

V

N

Q

G

G

C

I

F

P

R

K

G

N

K

V

R

A

I

I

P

P

V

V

A

G

G

Q

L

L

S

E

K

K

-

A

L

L

E

G

K

P

G

G

C

I

Y

H

F

F

P

D

A

G

S

S

V

S

F

I

A

E

M

-

D

-

I

-

L

-

G

-

N

-

V

-

E

-

A

-

G

-

L

-

G

G

Q

S

D

D

M

M

G

V

-

L

E

R

P

P

D

D

R

P

S

D

C

T

I

F

P

R

V

V

P

-

G

-

T

-

L

L

T

P

P

W

S

S

A

T

A

A

D

E

P

A

Q

R

S

L

I

I

A

C

Q

D

V

I

Q

E

L

L

T

P

M

-

V

-

D

-

E

-

D

D

G

G

A

K

P

P

F

F

D

M

V

G

E

C

P

P

R

R

N

Q

V

V

L

L

N

K

R

K

L

N

W

M

Q

E

L

A

R

A

Q

K

R

L

G

G

L

Y

F

V

P

M

V

N

V

T

A
x
G

V

P

E
|
E

L

M

E

E

F

F

Y

F

L

L

L

F

D

K

R

R

K

Q

R

D

D

G

A

-

E

-

G

-

Y

-

L

-

Q

M

P

P

P

T

C

N

A

I

P

P

G

-

T

-

D

Q

D

D

R

R

N

G

T

G

Q

Y

S

F

Q

D

V

L

Y

A

S

P

V

I

D

D

N

L

L

A

N

E

H

E

F

I

A

K

-

R

D

E

V

I

L

V

Y

L

D

V

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

V

D
x
E

G

A

A

A

V

H

A

H

E

E

A

V

S

A

P

F

G

G

Q

Q

F

H

E

E

I

I

N

D

L
x
F

H
x
K

H
x
Y

T

-

D

D

N

N

V

A

L

L

D

A

A

T

C

A

D

D

D

N

A

V

L

I

A

T

L

L

K

K

R

Y

L

V

V

A

R

K

L

T

M

L

A

A

E

L

K

Q

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

I

E

F

E

G

H

V

A

N

G

G

S

S

G

G

M

M

H
|
H

I

T

H
x
N

I

T

S
|
S

M

L

L

F

N

K

N

D

K

-

G

G

E

K

N

N

V

A

L

F

V

Y

D

D

G

P

D

D

G

A

E

P

D

D

-

Q

-

I

S

S

A

D

L

T

L

L

K

R

R

Y

A

F

L

V

A

G

G

G

M

V

I

L

D

K

L

H

M

I

P

R

A

A

S

I

M

T

A

A

L

I

L

T

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

V

P

P

G

G

M

Y

Y

E

V

A

P

P

T

V

Q

Y

A

I

S

T

W

W

G

S

H

G

N

P

N

N

R

R

T

S

V

S

A

L

L

I

R

R

I

V

P

P

C

A

G

P

E

R

R

G

Q

N

N
x
S

H

T

R
|
R

V

I

E
|
E

Y

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

V

A

M

F

A

A

I

I

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

V

D

K

N

N

P

K

Q

I

L

E

P

P

L

-

Q

P

E

E

E

R

V

V

E

E

G

K

N

N

binding adenosine-5'-triphosphate: G117 (≠ A157), E170 (≠ D220), F185 (≠ L235), K186 (≠ H236), Y187 (≠ H237), N233 (≠ H284), S235 (= S286), S315 (≠ N365), R317 (= R367)
binding magnesium ion: E119 (= E159), H231 (= H282), E319 (= E369)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
28% identity, 88% coverage: 36:453/473 of query aligns to 15:419/439 of 7tdpA

query
sites
7tdpA

D

N

T

V

Q

R

Y

F

V

I

D

R

V

L

L

Q

L

F

T

T

D

D

L

L

N

L

G

G

C

T

F

I

R

K

G

N

K

V

R

E

I

I

P

P

V

V

A

S

G

Q

L

L

S

E

K

K

-

A

L

L

E

D

K

N

G

K

C

M

Y

M

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

Y

V

V

V

R

E

I

E

E

A

-

G

-

L

-

G

-

Q

-

D

-

M

-

G

-

E

E

P

S

D

D

R

M

S

Y

C

L

I

Y

P

P

V

-

P

-

G

-

T

D

L

L

T

D

P

T

S

W

A

V

D

F

P

P

Q

W

S

S

-

D

-

R

I

V

A

A

Q

R

V

L

Q

I

L

C

T

D

M

I

V

Y

D

K

E

P

D

D

G

G

A

S

P

P

F

F

D

A

V

G

E

D

P

P

R

R

N

G

V

I

L

L

N

K

R

R

L

V

W

L

Q

K

Q

E

L

A

R

E

Q

E

R

L

G

G

L

Y

F

T

P

S

V

M

-
x
N

V

V

A
x
G

V

P

E
|
E

L

P

E
|
E

F

F

Y

F

L

L

L

F

D

-

R

-

K

K

R

T

D

D

A

E

E

K

G

G

Y

-

L

-

Q

-

P

-

C

-

A

-

P

D

G

P

T

T

D

T

D

E

R

L

N

N

T

D

Q

Q

S

G

Q

G

V

Y

Y

F

S

D

V

L

D

A

N

P

L

M

-

D

-

L

-

G

-

E

N

N

H

C

F

R

A

R

D

E

V

I

L

V

Y

L

D

K

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

I

D
x
E

G

A

A

S

V

H

A

H

E
|
E

A

V

S

A

P

P

G

G

Q

Q

F

H

E
|
E

I

I

N
x
D

L

F

H

K

H
x
Y

T

A

D

D

N

A

V

V

L

-

D

K

A

A

C

A

D

D

D

Q

A

I

L

Q

A

T

L

F

K

K

R

L

L

V

V

V

R

K

L

T

M

I

A

A

E

R

K

Q

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

L

E

F

E

G

H

V

A

N

G
|
G

S

S

G

G

M

M

H
|
H

I

C

H
x
N

I

Q

S
|
S

M

L

L

F

N

K

N

D

K

-

G

N

E

E

N

N

V

V

L

F

V

Y

D

D

G

E

D

T

G

D

E

E

D

L

-

G

-

L

S

S

A

Q

L

T

L

A

K

R

R

H

A

Y

L

M

A

A

G

G

M

I

I

L

D

K

L

H

M

A

P

R

A

A

S

M

M

A

A

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

V

P

P

G

G

M

Y

Y
x
E

V

A

P

P

T

C

Q

Y

A

V

S

A

W

W

G

S

H

A

N

S

N

N

R
|
R

T

S

V

P

A

M

L

I

R
|
R

I

I

P

P

C

A

G

S

E

R

R

G

Q

L

N

S

H

T

R
|
R

V

V

E
|
E

Y

V

R
|
R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

V

A

M

L

A

A

A

V

I

M

F

L

A

R

G

A

I

G

L

L

H

D

G

G

L

I

D

K

N

R

P

-

Q

Q

L

M

P

A

L

L

Q

P

E

A

E

P

V

I

E

D

G

R

N

N

G

I

L

Y

E

V

-

M

-

S

-

E

-

R

-

I

Q

E

E

E

G

G

L

I

P

P

-

S

F

L

P

P

I

A

R

D

Q

L

S

K

D

E

A

A

L

L

W

S

E

E

F

L

M

I

Q

R

N

S

D

E

H

V

L

I

R

S

E

D

R

A

L

L

G

G

E

D

R

H

F

A

C

L

H

A

V

Y

F

F

H

Y

A

E

C

L

K

K

H

E

D

I

E

E

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A157), E127 (= E159), E179 (≠ D220), D193 (≠ N234), Y196 (≠ H237), N242 (≠ H284), S244 (= S286), R316 (= R357), R326 (= R367)
binding magnesium ion: E127 (= E159), E127 (= E159), E129 (= E161), E184 (= E225), E191 (= E232), E191 (= E232), H240 (= H282), E328 (= E369)
binding l-methionine-s-sulfoximine phosphate: E127 (= E159), E129 (= E161), E184 (= E225), E191 (= E232), G236 (= G278), H240 (= H282), R293 (= R334), E299 (≠ Y340), R311 (= R352), R330 (= R371)

8ooxB Glutamine synthetase (see paper)
30% identity, 81% coverage: 26:408/473 of query aligns to 5:365/438 of 8ooxB

query
sites
8ooxB

E

E

V

I

K

F

R

R

Y

I

L

V

E

E

T

E

Y

-

P

K

D

N

T

V

Q

R

Y

F

V

V

D

R

V

L

L

Q

L

F

T

V

D

D

L

V

N

Q

G

G

C

I

F

P

R

K

G

N

K

V

R

A

I

I

P

P

V

V

A

G

G

Q

L

L

S

E

K

K

-

A

L

L

E

G

K

P

G

G

C

I

Y

H

F

F

P

D

A

G

S

S

V

-

F

-

A

-

M

-

D

S

I

I

L

E

G

G

N

F

V

V

V

R

E

I

E

E

A

-

G

-

L

-

G

-

Q

-

D

-

M

-

G

-

E

E

P

S

D

D

R

M

S

V

C

L

I

R

P

P

V

D

P

P

G

D

T

T

L

F

-

R

-

V

-

L

-

P

-

W

T

S

P

G

S

N

A

T

A

A

D

E

P

A

Q

R

S

L

I

I

A

C

Q

D

V

I

Q

E

L

L

T

P

M

-

V

-

D

-

E

-

D

D

G

G

A

K

P

P

F

F

D

M

V

G

E

C

P

P

R

R

N

Q

V

V

L

L

N

K

R

K

L

N

W

M

Q

E

L

A

R

A

Q

K

R

L

G

G

L

Y

F

V

P

M

V

N

V

T

A

G

V

P

E

E

L

M

E
|
E

F

F

Y

F

L

L

L

F

D

K

R

R

K

Q

R

D

D

G

A

-

E

-

G

-

Y

-

L

-

Q

M

P

P

P

T

C

N

A

I

P

P

G

-

T

-

D

Q

D

D

R

R

N

G

T

G

Q

Y

S

F

Q

D

V

L

Y

A

S

P

V

I

D

D

N

L

L

A

N

E

H

E

F

I

A

K

-

R

D

E

V

I

L

V

Y

L

D

V

I

L

D

E

E

E

L

M

A

G

K

-

L

-

Q

-

L

-

I

F

P

E

A

V

D

E

G

A

A

A

V

H

A

H

E
|
E

A

V

S

A

P

F

G

G

Q

Q

F

H

E
|
E

I

I

N

D

L

F

H

K

H

Y

T

-

D

D

N

N

V

A

L

L

D

A

A

T

C

A

D

D

D

N

A

V

L

I

A

T

L

L

K

K

R

Y

L

V

V

A

R

K

L

T

M

L

A

A

E

L

K

Q

H

H

K

G

M

L

H

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

I

E

F

E

G

H

V

A

N

G

G

S

S

G

G

M

M

H

H

I

T

H

N

I

T

S

S

M

L

L

F

N

K

N

D

K

-

G

G

E

K

N

N

V

A

L

F

V

Y

D

D

G

P

D

D

G

A

E

P

D

D

-

Q

-

I

S

S

A

D

L

T

L

L

K

R

R

Y

A

F

L

V

A

G

G

G

M

V

I

L

D

K

L

H

M

I

P

R

A

A

S

I

M

T

A

A

L

I

L

T

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

V

P

P

G

G

M

Y

Y

E

V

A

P

P

T

V

Q

Y

A

I

S

T

W

W

G

S

H

G

N

P

N

N

R

R

T

S

V

S

A

L

L

I

R

R

I

V

P

P

C

A

G

P

E

R

R

G

Q

N

N

S

H

T

R

R

V

I

E

E

Y

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

V

A

M

F

A

A

I

I

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

V

D

K

N

N

P

K

Q

I

L

E

P

P

L

-

Q

P

E

E

E

R

V

V

E

E

G

K

N

N

binding magnesium ion: E129 (= E161), E183 (= E225), E190 (= E232)

8wwuB Glutamine synthetase
28% identity, 87% coverage: 46:457/473 of query aligns to 40:465/492 of 8wwuB

query
sites
8wwuB

D

D

L

Q

N

Y

G

G

C

L

F

L

R

R

G

G

K

K

R

S

I

L

P

T

V

V

A

A

G

S

L

L

-

K

S

S

K

A

L

F

E

K

K

E

G

G

C

S

Y

E

F

V

P

A

A

I

S

G

V

P

F

F

A

F

M

F

D

D

I

L

L

V

G

S

-

S

-

M

-

V

-

F

N

N

V

L

V

F

E

T

A

A

G

G

L

D

G

F

Q

E

D

D

-

E

-

L

M

S

G

G

E

N

P

P

D

T

R

V

S

V

C

M

I

V

P

P

V

D

P

P

G

T

T

T

L

F

T

K

P

V

-

L

S

P

A

W

A

A

D

D

P

K

Q

T

S

G

I

W

A

M

Q

L

V

A

Q

D

L

L

T

H

M

W

V

-

D

-

E

K

D

S

G

G

A

E

P

P

F

F

D

P

V

L

E

C

P

P

R

R

N

G

V

I

L

M

N

K

R

K

L

A

W

V

Q

K

Q

S

L

L

R

S

Q

D

R

E

G

G

L

Y

F

L

P

F

V

K

V

C

A
x
G

V

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

L

V

D

D

R

R

K

S

R

L

D

S

A

P

E

E

G

S

Y

L

L

G

Q

A

P

P

P

G

C

V

A

Q

P

P

G

D

T

A

D

I

D

Q

R

V

N

Q

T

P

Q

V

S

A

Q

Q

V

G

Y

Y

S

S

V

V

D

-

N

L

L

L

N

E

H

H

F

H

A

L

D

D

V

Q

L

V

Y

D

D

D

I

I

D

M

E

S

L

K

A

V

K

R

L

K

Q

G

L

L

I

L

-

E

-

L

-

N

-

L

P

P

A

L

D
x
R

G

S

A

I

V

E

A

D

E
|
E

A

W

S

A

P

P

G

S

Q

Q

F

M

E
|
E

I

T

N

T

L
x
F

H

D

H
x
V

T

M

D

E

N

G

V

-

L

L

D

E

A

A

C

A

D

D

D

A

A

A

L

L

A

L

L

I

K

K

R

S

L

A

V

I

R

K

L

Q

M

I

A

C

E

S

K

R

H

H

K

G

M

Y

H

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

-

A

Y

I

E

N

E

G

H

F

A

F

G

A

S

S

G

G

M

W

H
|
H

I

M

H
|
H

I

Q

S
|
S

M

L

L

V

N

D

-

K

N

D

K

T

G

R

E

K

N

N

V

L

L

F

V

I

D

P

G

S

D

E

G

G

E

E

D

V

S

L

A

S

L

P

L

L

K

G

R

R

A

A

L

Y

A

A

G

G

-

G

M

L

I

L

D

A

L

N

M

G

P

S

A

A

S

A

M

S

A

S

L

F

L

T

A

T

P

P

N

T

V

V

N

N

S

G

Y

Y

R

R

F

R

Q

Q

P

P

G

Y

M

S

Y

L

V

A

P

P

T

D

Q

R

A

R

S

A

W

W

G

A

H

K

N

D

N

N

R
x
K

T

A

V

A

A

M

L

V

R
|
R

I

V

P

V

C

S

G

A

E

T

R

G

Q

D

-

P

N

A

H

S

R
|
R

V

I

E
|
E

Y

N

R

R

V

I

A

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

L

L

V

Y

M

M

A

A

A

S

I

Q

F

I

A

V

G

S

I

G

L

L

H

D

G

G

L

I

D

K

N

N

P

K

Q

K

L

D

P

P

L

-

Q

-

E

G

E

E

V

L

E

Q

G

E

N

S

G

P

L

Y

E

D

Q

A

E

Q

G

V

L

P

P

M

F

L

P

P

I

T

R

T

Q

L

S

A

D

E

A

A

L

L

W

-

E

D

F

A

M

L

Q

E

N

H

D

D

H

S

-

E

-

L

L

F

R

R

E

S

R

C

L

F

G

G

E

D

R

T

F

F

C

I

H

K

V

Y

F

W

H

L

A

Q

C

L

K

R

H

R

D

S

E

E

L

W

L

A

Q

R

F

F

binding phosphoaminophosphonic acid-adenylate ester: G157 (≠ A157), E159 (= E159), R226 (≠ D220), F241 (≠ L235), V243 (≠ H237), H290 (= H284), S292 (= S286), K360 (≠ R352), R365 (= R357), R376 (= R367)
binding magnesium ion: E159 (= E159), E238 (= E232)
binding manganese (ii) ion: E159 (= E159), E161 (= E161), E231 (= E225), E238 (= E232), H288 (= H282), E378 (= E369)

8wwvA Glutamine synthetase
28% identity, 87% coverage: 46:457/473 of query aligns to 38:463/490 of 8wwvA

query
sites
8wwvA

D

D

L

Q

N

Y

G

G

C

L

F

L

R

R

G

G

K

K

R

S

I

L

P

T

V

V

A

A

G

S

L

L

-

K

S

S

K

A

L

F

E

K

K

E

G

G

C

S

Y

E

F

V

P

A

A

I

S

G

V

P

F

F

A

F

M

F

D

D

I

L

L

V

G

S

-

S

-

M

-

V

-

F

N

N

V

L

V

F

E

T

A

A

G

G

L

D

G

F

Q

E

D

D

-

E

-

L

M

S

G

G

E

N

P

P

D

T

R

V

S

V

C

M

I

V

P

P

V

D

P

P

G

T

T

T

L

F

T

K

P

V

-

L

S

P

A

W

A

A

D

D

P

K

Q

T

S

G

I

W

A

M

Q

L

V

A

Q

D

L

L

T

H

M

W

V

-

D

-

E

K

D

S

G

G

A

E

P

P

F

F

D

P

V

L

E

C

P

P

R

R

N

G

V

I

L

M

N

K

R

K

L

A

W

V

Q

K

Q

S

L

L

R

S

Q

D

R

E

G

G

L

Y

F

L

P

F

V

K

V

C

A
x
G

V

I

E
|
E

L

L

E
|
E

F

W

Y

Y

L

L

-

T

-

K

-

I

L

V

D

D

R

R

K

S

R

L

D

S

A

P

E

E

G

S

Y

L

L

G

Q

A

P

P

P

G

C

V

A

Q

P

P

G

D

T

A

D

I

D

Q

R

V

N

Q

T

P

Q

V

S

A

Q

Q

V

G

Y

Y

S

S

V

V

D

-

N

L

L

L

N

E

H

H

F

H

A

L

D

D

V

Q

L

V

Y

D

D

D

I

I

D

M

E

S

L

K

A

V

K

R

L

K

Q

G

L

L

I

L

-

E

-

L

-

N

-

L

P

P

A

L

D
x
R

G

S

A

I

V

E

A

D

E
|
E

A

W

S

A

P

P

G

S

Q

Q

F

M

E
|
E

I

T

N

T

L
x
F

H
x
D

H
x
V

T

M

D

E

N

G

V

-

L

L

D

E

A

A

C

A

D

D

D

A

A

A

L

L

A

L

L

I

K

K

R

S

L

A

V

I

R

K

L

Q

M

I

A

C

E

S

K

R

H

H

K

G

M

Y

H

H

A

A

T

T

F

F

M

M

A

C

K

K

P

P

-

A

Y

I

E

N

E

G

H

F

A

F

G
x
A

S

S

G

G

M

W

H
|
H

I

M

H
|
H

I

Q

S
|
S

M

L

L

V

N

D

-

K

N

D

K

T

G

R

E

K

N

N

V

L

L

F

V

I

D

P

G

S

D

E

G

G

E

E

D

V

S

L

A

S

L

P

L

L

K

G

R

R

A

A

L

Y

A

A

G

G

-

G

M

L

I

L

D

A

L

N

M

G

P

S

A

A

S

A

M

S

A

S

L

F

L

T

A

T

P

P

N

T

V

V

N

N

S

G

Y

Y

R

R

R
|
R

F

R

Q

Q

P

P

G

Y

M

S

Y

L

V

A

P

P

T

D

Q

R

A

R

S

A

W

W

G

A

H

K

N

D

N

N

R
x
K

T

A

V

A

A

M

L

V

R

R

I

V

P

V

C

S

G

A

E

T

R

G

Q

D

-

P

N

A

H

S

R
|
R

V

I

E
|
E

Y

N

R

R

V

I

A

G

G

E

A

P

D

G

A

A

N

N

P

P

Y

Y

L

L

V

Y

M

M

A

A

A

S

I

Q

F

I

A

V

G

S

I

G

L

L

H

D

G

G

L

I

D

K

N

N

P

K

Q

K

L

D

P

P

L

-

Q

-

E

G

E

E

V

L

E

Q

G

E

N

S

G

P

L

Y

E

D

Q

A

E

Q

G

V

L

P

P

M

F

L

P

P

I

T

R

T

Q

L

S

A

D

E

A

A

L

L

W

-

E

D

F

A

M

L

Q

E

N

H

D

D

H

S

-

E

-

L

L

F

R

R

E

S

R

C

L

F

G

G

E

D

R

T

F

F

C

I

H

K

V

Y

F

W

H

L

A

Q

C

L

K

R

H

R

D

S

E

E

L

W

L

A

Q

R

F

F

binding adenosine-5'-diphosphate: G155 (≠ A157), E157 (= E159), R224 (≠ D220), F239 (≠ L235), D240 (≠ H236), V241 (≠ H237), H288 (= H284), S290 (= S286), R374 (= R367), E376 (= E369)
binding magnesium ion: E157 (= E159), E236 (= E232)
binding manganese (ii) ion: E157 (= E159), E159 (= E161), E229 (= E225), E236 (= E232), H286 (= H282), E376 (= E369)
binding l-methionine-s-sulfoximine phosphate: E157 (= E159), E159 (= E161), E229 (= E225), E236 (= E232), A282 (≠ G278), H286 (= H282), R340 (= R334), K358 (≠ R352)

7cqwA Gmas/adp complex-conformation 1 (see paper)
30% identity, 80% coverage: 38:417/473 of query aligns to 14:378/430 of 7cqwA

query
sites
7cqwA

Q

E

Y

F

V

F

D

L

V

I

L

S

L

F

T

T

D

D

L

L

N

L

G

G

C

V

F

Q

R

R

G

A

K

K

R

L

I

V

P

P

V

A

A

R

G

A

L

I

S

A

K

-

L

-

E

-

K

-

G

-

C

-

Y

-

F

-

P

-

A

-

S

-

V

-

F

-

A

-

M

-

D

D

I

M

L

A

G

V

N

N

V

G

V

A

E

G

E

F

A

A

G

G

L

F

G

A

Q

A

-

W

-

L

D

D

M

M

G

S

E

P

P

A

D

D

R

A

S

D

C

I

I

L

P

A

V

I

P

P

G

-

T

-

L

-

T

-

P

-

S

-

A

-

D

D

P

P

Q

E

S

S

I

L

A

I

Q

Q

V

L

Q

P

L

W

-

K

-

P

-

S

-

V

-

G

-

W

-

L

-

A

T

A

M

D

V

V

D

H

E

F

D

E

G

G

A

R

P

P

F

F

D

P

V

K

E

A

P

P

R

R

N

V

V

A

L

L

N

K

R

S

L

V

W

L

Q

A

Q

R

L

A

R

A

Q

G

R

K

G

D

L

M

F

H

P

L

V

K

V

H

A

G

V

V

E

E

L

C

E

E

F

F

Y

F

L

L

L

I

D

-

R

-

K

-

R

-

D

-

A

-

E

-

G

-

Y

-

L

-

Q

Q

P

P

P

D

C

G

A

S

P

A

G

I

T

S

D

D

D

P

R

A

N

D

T

T

Q

Q

S

A

Q

K

-

P

V

C

Y

Y

S

D

V

Q

D

D

N

A

L

L

N

M

H

R

F

R

A

F

D

D

V

V

L

I

Y

A

D

E

I

I

-

C

D

S

E

Y

L

M

A

V

K

D

L

L

Q

G

L

W

I

G

P

P

A
x
Y

D

Q

G

N

A

D

V

H

A

E

E

D

A

A

S

N

P

-

G

G

Q

Q

F

F

E

E

I

M

N

N

L

W

H

D

H
x
Y

T

A

D

D

N

A

V

L

L

V

D

T

A

A

C

D

D

R

D

H

A

A

L

F

A

-

L

F

K

K

R

F

L

M

V

V

R

K

L

S

M

V

A

A

E

E

K

R

H

H

K

G

M

L

H

R

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

E

A

E

H

H

L

A

T

G

G

S

N

G

G

M

C

H

H

I

T

H
|
H

I
x
L

S
|
S

M

M

L

W

N

T

N

A

K

A

G

G

E

D

N

N

V

-

L

L

V

F

D

E

G

G

D

D

G

G

E

E

-

L

-

G

D

L

S

S

A

P

L

T

L

A

K

Y

R

A

A

F

L

L

A

G

G

G

M

L

I

I

D

G

L

H

M

A

P

K

A

G

S

L

M

T

A

A

L

V

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

F

L

Q

N

P

A

-

P

-

V

-

T

-

V

-

S

-

G

G

A

M

T

Y

W

V

S

P

P

T

N

Q

T

A

I

S

T

W

Y

G

G

H

G

N

N

R

R

T

T

V

H

A

M

L

V

R
|
R

I

I

P

P

C

-

G

-

E

-

R

-

Q

D

N

A

H

G

R
|
R

V

L

E

E

Y

L

R

R

V

L

A

P

G

D

A

G

D

A

A

A

N

N

P

P

Y

Y

L

L

V

M

M

P

A

A

I

I

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

E

N

T

P

Q

Q

A

L

D

P

P

L

G

Q

Q

E

R

-

L

-

D

-

I

-

D

-

M

E

Y

V

V

E

E

G

G

N

H

G

S

L

V

E

E

Q

A

E

E

G

Q

L

L

P

P

F

L

binding adenosine-5'-diphosphate: Y174 (≠ A219), Y191 (≠ H237), H237 (= H284), L238 (≠ I285), S239 (= S286), R317 (= R357), R323 (= R367)

7cquA Gmas/adp/metsox-p complex (see paper)
30% identity, 80% coverage: 38:417/473 of query aligns to 13:377/429 of 7cquA

query
sites
7cquA

Q

E

Y

F

V

F

D

L

V

I

L

S

L

F

T

T

D

D

L

L

N

L

G

G

C

V

F

Q

R

R

G

A

K

K

R

L

I

V

P

P

V

A

A

R

G

A

L

I

S

A

K

-

L

-

E

-

K

-

G

-

C

-

Y

-

F

-

P

-

A

-

S

-

V

-

F

-

A

-

M

-

D

D

I

M

L

A

G

V

N

N

V

G

V

A

E

G

E

F

A

A

G

G

L

F

G

A

Q

A

-

W

-

L

D

D

M

M

G

S

E

P

P

A

D

D

R

A

S

D

C

I

I

L

P

A

V

I

P

P

G

-

T

-

L

-

T

-

P

-

S

-

A

-

D

D

P

P

Q

E

S

S

I

L

A

I

Q

Q

V

L

Q

P

L

W

-

K

-

P

-

S

-

V

-

G

-

W

-

L

-

A

T

A

M

D

V

V

D

H

E

F

D

E

G

G

A

R

P

P

F

F

D

P

V

K

E

A

P

P

R

R

N

V

V

A

L

L

N

K

R

S

L

V

W

L

Q

A

Q

R

L

A

R

A

Q

G

R

K

G

D

L

M

F

H

P

L

V

K

V

H

A

G

V

V

E
|
E

L

C

E
|
E

F

F

Y

F

L

L

L

I

D

-

R

-

K

-

R

-

D

-

A

-

E

-

G

-

Y

-

L

-

Q

Q

P

P

P

D

C

G

A

S

P

A

G

I

T

S

D

D

D

P

R

A

N

D

T

T

Q

Q

S

A

Q

K

-

P

V

C

Y

Y

S

D

V

Q

D

D

N

A

L

L

N

M

H

R

F

R

A

F

D

D

V

V

L

I

Y

A

D

E

I

I

-

C

D

S

E

Y

L

M

A

V

K

D

L

L

Q

G

L

W

I

G

P

P

A
x
Y

D

Q

G

N

A

D

V

H

A
x
E

E

D

A

A

S

N

P

-

G

G

Q

Q

F

F

E
|
E

I

M

N
|
N

L
x
W

H
x
D

H
x
Y

T

A

D

D

N

A

V

L

L

V

D

T

A

A

C

D

D

R

D

H

A

A

L

F

A

-

L

F

K

K

R

F

L

M

V

V

R

K

L

S

M

V

A

A

E

E

K

R

H

H

K

G

M

L

H

R

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

E

A

E

H

H

L

A
x
T

G
|
G

S

N

G

G

M

C

H
|
H

I

T

H
|
H

I
x
L

S
|
S

M

M

L

W

N

T

N

A

K

A

G

G

E

D

N

N

V

-

L

L

V

F

D

E

G

G

D

D

G

G

E

E

-

L

-

G

D

L

S

S

A

P

L

T

L

A

K

Y

R

A

A

F

L

L

A

G

G

G

M

L

I

I

D

G

L

H

M

A

P

K

A

G

S

L

M

T

A

A

L

V

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

N

P

A

-

P

-

V

-

T

-

V

-

S

-

G

G

A

M

T

Y
x
W

V

S

P

P

T

N

Q

T

A

I

S

T

W

Y

G

G

H

G

N

N

R
|
R

T

T

V

H

A

M

L

V

R
|
R

I

I

P

P

C

-

G

-

E

-

R

-

Q

D

N

A

H

G

R
|
R

V

L

E
|
E

Y

L

R
|
R

V

L

A

P

G

D

A

G

D

A

A

A

N

N

P

P

Y

Y

L

L

V

M

M

P

A

A

I

I

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

E

N

T

P

Q

Q

A

L

D

P

P

L

G

Q

Q

E

R

-

L

-

D

-

I

-

D

-

M

E

Y

V

V

E

E

G

G

N

H

G

S

L

V

E

E

Q

A

E

E

G

Q

L

L

P

P

F

L

binding adenosine-5'-diphosphate: E121 (= E159), Y173 (≠ A219), N187 (= N234), W188 (≠ L235), D189 (≠ H236), Y190 (≠ H237), H236 (= H284), L237 (≠ I285), S238 (= S286), R316 (= R357), R322 (= R367)
binding magnesium ion: E121 (= E159), E121 (= E159), E123 (= E161), E178 (≠ A224), E185 (= E232), E185 (= E232), H234 (= H282), E324 (= E369)
binding l-methionine-s-sulfoximine phosphate: E121 (= E159), E123 (= E161), E178 (≠ A224), E185 (= E232), T229 (≠ A277), G230 (= G278), H234 (= H282), R287 (= R334), W299 (≠ Y340), R311 (= R352), R326 (= R371)

7cqqA Gmas in complex with amppnp and metsox (see paper)
30% identity, 80% coverage: 38:417/473 of query aligns to 13:377/429 of 7cqqA

query
sites
7cqqA

Q

E

Y

F

V

F

D

L

V

I

L

S

L

F

T

T

D

D

L

L

N

L

G

G

C

V

F

Q

R

R

G

A

K

K

R

L

I

V

P

P

V

A

A

R

G

A

L

I

S

A

K

-

L

-

E

-

K

-

G

-

C

-

Y

-

F

-

P

-

A

-

S

-

V

-

F

-

A

-

M

-

D

D

I

M

L

A

G

V

N

N

V

G

V

A

E

G

E

F

A

A

G

G

L

F

G

A

Q

A

-

W

-

L

D

D

M

M

G

S

E

P

P

A

D

D

R

A

S

D

C

I

I

L

P

A

V

I

P

P

G

-

T

-

L

-

T

-

P

-

S

-

A

-

D

D

P

P

Q

E

S

S

I

L

A

I

Q

Q

V

L

Q

P

L

W

-

K

-

P

-

S

-

V

-

G

-

W

-

L

-

A

T

A

M

D

V

V

D

H

E

F

D

E

G

G

A

R

P

P

F

F

D

P

V

K

E

A

P

P

R

R

N

V

V

A

L

L

N

K

R

S

L

V

W

L

Q

A

Q

R

L

A

R

A

Q

G

R

K

G

D

L

M

F

H

P

L

V

K

V

H

A

G

V

V

E
|
E

L

C

E
|
E

F

F

Y

F

L

L

L

I

D

-

R

-

K

-

R

-

D

-

A

-

E

-

G

-

Y

-

L

-

Q

Q

P

P

P

D

C

G

A

S

P

A

G

I

T

S

D

D

D

P

R

A

N

D

T

T

Q

Q

S

A

Q

K

-

P

V

C

Y

Y

S

D

V

Q

D

D

N

A

L

L

N

M

H

R

F

R

A

F

D

D

V

V

L

I

Y

A

D

E

I

I

-

C

D

S

E

Y

L

M

A

V

K

D

L

L

Q

G

L

W

I

G

P

P

A
x
Y

D

Q

G

N

A

D

V

H

A
x
E

E

D

A

A

S

N

P

-

G

G

Q

Q

F

F

E
|
E

I

M

N
|
N

L

W

H
x
D

H
x
Y

T

A

D

D

N

A

V

L

L

V

D

T

A

A

C

D

D

R

D

H

A

A

L

F

A

-

L

F

K

K

R

F

L

M

V

V

R

K

L

S

M

V

A

A

E

E

K

R

H

H

K

G

M

L

H

R

A

A

T

T

F

F

M

M

A

P

K

K

P

P

Y

F

E

A

E

H

H

L

A
x
T

G

G

S

N

G

G

M

C

H
|
H

I

T

H
|
H

I

L

S
|
S

M

M

L

W

N

T

N

A

K

A

G

G

E

D

N

N

V

-

L

L

V

F

D

E

G

G

D

D

G

G

E

E

-

L

-

G

D

L

S

S

A

P

L

T

L

A

K

Y

R

A

A

F

L

L

A

G

G

G

M

L

I

I

D

G

L

H

M

A

P

K

A

G

S

L

M

T

A

A

L

V

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

F

L

Q

N

P

A

-

P

-

V

-

T

-

V

-

S

-

G

G

A

M

T

Y
x
W

V

S

P

P

T

N

Q

T

A

I

S

T

W

Y

G

G

H

G

N

N

R
|
R

T

T

V

H

A

M

L

V

R
|
R

I

I

P

P

C

-

G

-

E

-

R

-

Q

D

N

A

H

G

R
|
R

V

L

E
|
E

Y

L

R
|
R

V

L

A

P

G

D

A

G

D

A

A

A

N

N

P

P

Y

Y

L

L

V

M

M

P

A

A

I

I

F

L

A

A

G

A

I

G

L

L

H

D

G

G

L

I

D

E

N

T

P

Q

Q

A

L

D

P

P

L

G

Q

Q

E

R

-

L

-

D

-

I

-

D

-

M

E

Y

V

V

E

E

G

G

N

H

G

S

L

V

E

E

Q

A

E

E

G

Q

L

L

P

P

F

L

binding phosphoaminophosphonic acid-adenylate ester: E121 (= E159), Y173 (≠ A219), E185 (= E232), N187 (= N234), D189 (≠ H236), Y190 (≠ H237), H234 (= H282), H236 (= H284), S238 (= S286), R311 (= R352), R316 (= R357), R322 (= R367), E324 (= E369)
binding magnesium ion: E121 (= E159), E121 (= E159), E123 (= E161), E178 (≠ A224), E185 (= E232), E185 (= E232), H234 (= H282), E324 (= E369)
binding (2s)-2-amino-4-(methylsulfonimidoyl)butanoic acid: E123 (= E161), E178 (≠ A224), T229 (≠ A277), H234 (= H282), R287 (= R334), W299 (≠ Y340), R311 (= R352), R326 (= R371)

Query Sequence

>WP_012968595.1 NCBI__GCF_000025465.1:WP_012968595.1
METNIVEVENFVQQTEERRVSAFAKEVKRYLETYPDTQYVDVLLTDLNGCFRGKRIPVAG
LSKLEKGCYFPASVFAMDILGNVVEEAGLGQDMGEPDRSCIPVPGTLTPSAADPQSIAQV
QLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVELEFYLLDRKRDAEGYLQPPCA
PGTDDRNTQSQVYSVDNLNHFADVLYDIDELAKLQLIPADGAVAEASPGQFEINLHHTDN
VLDACDDALALKRLVRLMAEKHKMHATFMAKPYEEHAGSGMHIHISMLNNKGENVLVDGD
GEDSALLKRALAGMIDLMPASMALLAPNVNSYRRFQPGMYVPTQASWGHNNRTVALRIPC
GERQNHRVEYRVAGADANPYLVMAAIFAGILHGLDNPQLPLQEEVEGNGLEQEGLPFPIR
QSDALWEFMQNDHLRERLGERFCHVFHACKHDELLQFERLITETEIEWMLKNA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory