SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012970085.1 NCBI__GCF_000025485.1:WP_012970085.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7bw9A Crystal structure of serine acetyltransferase isoform 3 in complex with cysteine from entamoeba histolytica
50% identity, 57% coverage: 5:154/265 of query aligns to 103:252/280 of 7bw9A

query
sites
7bw9A

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binding cysteine: D114 (= D16), P115 (= P17), D179 (= D81), H180 (= H82), A206 (≠ G108), R214 (= R116), H215 (= H117)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
47% identity, 63% coverage: 8:173/265 of query aligns to 87:253/272 of 3gvdI

query
sites
3gvdI

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binding cysteine: D95 (= D16), P96 (= P17), D160 (= D81), G186 (= G107), G187 (= G108), R195 (= R116)

4h7oA Crystal structure of serine acetyltransferase from vibrio cholerae o1 biovar el tor n16961
46% identity, 63% coverage: 8:173/265 of query aligns to 80:246/258 of 4h7oA

query
sites
4h7oA

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binding arginine: Y127 (≠ V55), Q131 (≠ I59)
binding cysteine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

Sites not aligning to the query:

binding arginine: 51, 53

1ssqD Serine acetyltransferase- complex with cysteine (see paper)
45% identity, 63% coverage: 8:174/265 of query aligns to 80:246/257 of 1ssqD

query
sites
1ssqD

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binding cysteine: D88 (= D16), D153 (= D81), H154 (= H82)

4hzdA Crystal structure of serine acetyltransferase in complex with coenzyme a from brucella abortus strain s19 (see paper)
43% identity, 62% coverage: 5:168/265 of query aligns to 81:244/250 of 4hzdA

query
sites
4hzdA

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binding coenzyme a: H158 (= H82), G203 (= G127), A204 (= A128), A221 (≠ G145), A222 (≠ S146), T235 (= T159), A237 (≠ V161), I244 (= I168)
binding magnesium ion: S224 (≠ A148), A237 (≠ V161), G238 (= G162)

8i04A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with serine (see paper)
47% identity, 61% coverage: 8:168/265 of query aligns to 80:240/258 of 8i04A

query
sites
8i04A

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binding serine: D88 (= D16), P89 (= P17), D153 (= D81), H154 (= H82), G180 (= G108), R188 (= R116), H189 (= H117)

8i09A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with butyl gallate (see paper)
47% identity, 61% coverage: 8:168/265 of query aligns to 83:243/246 of 8i09A

query
sites
8i09A

D

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binding cysteine: D91 (= D16), D156 (= D81), H157 (= H82), G164 (= G89), R191 (= R116), H192 (= H117)
binding butyl 3,4,5-tris(oxidanyl)benzoate: G183 (= G108), T184 (= T109), G202 (= G127), K218 (≠ R143), I219 (= I144), G220 (= G145), L226 (≠ V151), A236 (≠ V161)

8i06A Crystal structure of serine acetyltransferase from salmonella typhimurium complexed with coa (see paper)
47% identity, 61% coverage: 8:168/265 of query aligns to 84:244/244 of 8i06A

query
sites
8i06A

D

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R

D

N

K

A

Y

F

S

E

T

I

P

L

L

S

L

T

Y

Y

L

P

K

G

G

L

F

H

H

A

A

V

L

M

Q

V

A

H

Y

R

R

I

I

A

G

H

H

R

W

L

L

W

W

R

N

R

K

D

G

F

R

K

R

W

A

S

L

A

A

R

I

V

F

L

L

S

Q

N

N

I

Q

A

V

R

S

L

V

F

S

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

R

R

R

G

F

I

F

M

I

L

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

V

V

I

I

G

E

D

D

D

D

C

V

T

S

L

I

Y
x
L

H
x
Q

G

S

V

V

T

T

L

L

G

G

G

G

T

T

S

G

W

K

Q

T

K

S

G

G

K

D

R

R

H

H

P

P

T

K

L

I

G

R

R

E

D

G

V

V

V

M

V

I

G
|
G

A
|
A

G

G

A

A

K

K

V

I

L

L

G

G

P

N

I

I

E

E

I

V

G

G

D

R

G

G

A

A

R

K

I

I

G
|
G

S
x
A

N

G

A

S

V

V

V

V

V

L

K

Q

A

P

V

V

P

P

P

P

G

H

A

T

T
|
T

A

A

V
x
A

G

G

V

V

P

P

G

A

R

R

I

I

I

V

binding coenzyme a: H158 (= H82), L177 (≠ Y101), Q178 (≠ H102), G203 (= G127), A204 (= A128), G221 (= G145), A222 (≠ S146), T235 (= T159), A237 (≠ V161)
binding cysteine: P93 (= P17), H158 (= H82)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
46% identity, 63% coverage: 8:173/265 of query aligns to 84:250/262 of 1t3dA

query
sites
1t3dA

D

D

I

I

L

Q

C

A

V

V

F

R

D

T

R

R

D
|
D

P
|
P

A

A

A

V

R

D

N

K

A

Y

F

S

E

T

I

P

L

L

S

L

T

Y

Y

L

P

K

G

G

L

F

H

H

A

A

V

L

M

Q

V

A

H

Y

R

R

I

I

A

G

H

H

R

W

L

L

W

W

R

N

R

Q

D

G

F

R

K

R

W

A

S

L

A

A

R

I

V

F

L

L

S

Q

N

N

I

Q

A

V

R

S

L

V

F

T

T

F

G

Q

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

R

R

R

G

F

I

F

M

I

L

D
|
D

H

H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

A

V

V

I

I

G

E

D

N

D

D

C

V

T

S

L

I

Y

L

H

Q

G

S

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

W

K

Q

S

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

R

R

E

D

G

V

V

V

M

V

I

G

G

A

A

G

G

A

A

K

K

V

I

L

L

G

G

P

N

I

I

E

E

I

V

G

G

D

R

G

G

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

V

L

K

Q

A

P

V

V

P

P

P

P

G

H

A

T

T

T

A

A

V

A

G

G

V

V

P

P

G

A

R

R

I

I

I

V

-

G

E

K

P

P

E

D

R

S

D

D

binding cysteine: D92 (= D16), P93 (= P17), D157 (= D81), G184 (= G108), R192 (= R116), H193 (= H117)

4n69A Soybean serine acetyltransferase complexed with serine (see paper)
47% identity, 61% coverage: 8:168/265 of query aligns to 83:243/243 of 4n69A

query
sites
4n69A

D

D

I

L

L

R

C

A

V

A

F

R

D

E

R

R

D

D

P

P

A

A

A

C

R

V

N

S

A

Y

F

S

E

H

I

C

L

L

S

L

T

N

Y

Y

P

K

G

G

L

F

H

L

A

A

V

C

M

Q

V

A

H

H

R

R

I

V

A

A

H

H

R

L

L

L

W

W

R

R

R

Q

D

S

F

R

K

R

W

P

S

L

A

A

R

L

V

A

L

L

-

H

S

S

N

R

I

I

A

A

R

N

L

V

F

F

T

A

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H

H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

I

I

G

G

D

N

D

N

C

V

T

S

L

I

Y

L

H

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

W

K

Q

V

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

R

D

D

G

V

V

V

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

P

N

I

I

E

K

I

I

G

G

D

E

G

G

A

A

R

K

I

V

G

G

S

A

N

G

A

S

V

V

V

V

V

L

K

I

A

D

V

V

P

P

P

P

G

R

A

T

T

T

A

A

V

V

G

G

V

N

P

P

G

A

R

R

I

L

I

V

binding serine: G183 (= G108), R191 (= R116), H192 (= H117)

6wyeA Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) (see paper)
43% identity, 61% coverage: 5:166/265 of query aligns to 82:243/261 of 6wyeA

query
sites
6wyeA

I

V

R

E

E

A

D

D

I

L

L

K

C

A

V

I

F

Y

D

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

N

E

A

Y

F

S

E

L

I

P

L

L

S

L

T

Y

Y

F

P

K

G

G

L

F

H

H

A

A

V

I

M

Q

V

A

H

H

R

R

I

I

A

N

H

H

R

R

L

L

W

Y

R

L

R

D

D

G

F

R

K

K

W

T

S

L

A

A

R

Y

V

F

L

L

S

Q

N

N

I

R

A

M

R

S

L

E

F

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

L

G

G

R

Y

R

G

F

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

I

A

G

G

E

E

T

T

A

A

V

V

I

L

G

G

D

N

D

N

C

I

T

S

L

I

Y

L

H

H

G

G

V

V

T

T

L

L

G

G

G
|
G

T

S

S

G

W

K

Q

E

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

R

D

D

G

V

V

V

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

P

N

I

I

E

R

I

I

G

G

D

S

G

N

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

V

V

K

S

A

D

V

V

P

P

P

P

G

S

A

I

T

T

A

V

V

V

G

G

V

V

P

P

G

A

R

K

binding (2S)-2-hydroxybutanedioic acid: D93 (= D16), P94 (= P17), D158 (= D81), H159 (= H82), G185 (= G108), R193 (= R116), H194 (= H117)

7ra4A Crystal structure of neisseria gonorrhoeae serine acetyltransferase (cyse) in complex with serine (see paper)
43% identity, 61% coverage: 5:166/265 of query aligns to 80:241/243 of 7ra4A

query
sites
7ra4A

I

V

R

E

E

A

D

D

I

L

L

K

C

A

V

I

F

Y

D

E

R

R

D
|
D

P
|
P

A

A

A

C

R

D

N

E

A

Y

F

S

E

L

I

P

L

L

S

L

T

Y

Y

F

P

K

G

G

L

F

H

H

A

A

V

I

M

Q

V

A

H

H

R

R

I

I

A

N

H

H

R

R

L

L

W

Y

R

L

R

D

D

G

F

R

K

K

W

T

S

L

A

A

R

Y

V

F

L

L

S

Q

N

N

I

R

A

M

R

S

L

E

F

V

T

F

G

G

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

L

G

G

R

Y

R

G

F

L

F

M

I

L

D
|
D

H
|
H

G

A

M

T

G

G

V

F

V

V

I

A

G

G

E

E

T

T

A

A

V

V

I

L

G

G

D

N

D

N

C

I

T

S

L

I

Y

L

H

H

G

G

V

V

T

T

L

L

G

G

G

G

T

S

S

G

W

K

Q

E

K

G

G

G

K

D

R
|
R

H
|
H

P

P

T

K

L

I

G

G

R

D

D

G

V

V

V

M

V

I

G

G

A

A

G

N

A

A

K

S

V

I

L

L

G

G

P

N

I

I

E

R

I

I

G

G

D

S

G

N

A

A

R

K

I

I

G

G

S

A

N

G

A

S

V

V

V

V

V

V

K

S

A

D

V

V

P

P

P

P

G

S

A

I

T

T

A

V

V

V

G

G

V

V

P

P

G

A

R

K

binding serine: D91 (= D16), P92 (= P17), D156 (= D81), H157 (= H82), R191 (= R116), H192 (= H117)

3q1xA Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-serine (see paper)
43% identity, 59% coverage: 5:160/265 of query aligns to 103:266/267 of 3q1xA

query
sites
3q1xA

I

L

R

K

E

T

D

D

I

L

L

I

C

A

V

A

F

Y

D

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

A

L

F

S

E

L

I

I

L

I

S

R

T

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

V

V

M

I

V

V

H

Y

R

R

I

I

A

A

H

H

R

V

L

L

W

Y

R

E

R

C

D

G

F

E

K

R

W

Y

S

Y

A

C

R

R

V

E

L

M

S

M

N

E

I

S

A

V

R

H

L

S

F

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

V

S

I

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

I

I

G

G

D

E

D

W

C

C

T

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G

A

T

M

S
x
H

W

F

Q

Q

K

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

R

D

D

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

P

N

I

I

E

I

I

V

G

G

D

S

G

H

A

V

R

R

I

I

G

G

S

A

N

N

A

C

V

W

V

I

V

D

K

R

A

D

V

V

P

D

P

S

G

N

A

Q

T

T

A

V

binding serine: H208 (≠ S110), R222 (= R116), H223 (= H117)

3p47A Crystal structure of entamoeba histolytica serine acetyltransferase 1 in complex with l-cysteine (see paper)
43% identity, 59% coverage: 5:160/265 of query aligns to 105:268/270 of 3p47A

query
sites
3p47A

I

L

R

K

E

T

D

D

I

L

L

I

C

A

V

A

F

Y

D

A

R

G

D

D

P

P

A

A

A

A

R

P

N

G

A

L

F

S

E

L

I

I

L

I

S

R

T

C

Y

Y

P

P

G

G

L

F

H

Q

A

A

V

V

M

I

V

V

H

Y

R

R

I

I

A

A

H

H

R

V

L

L

W

Y

R

E

R

C

D

G

F

E

K

R

W

Y

S

Y

A

C

R

R

V

E

L

M

S

M

N

E

I

S

A

V

R

H

L

S

F

Y

T

T

G

S

I

I

E

D

I

I

H

H

P

P

G

G

A

A

V

S

I

I

G

K

R

G

R

H

F

F

F

F

I

I

D

D

H

H

G

G

M

V

G

G

V

V

V

V

I

I

G

G

E

E

T

T

A

A

V

I

I

I

G

G

D

E

D

W

C

C

T

R

L

I

Y

Y

H

Q

G

S

V

V

T

T

L

L

G

G

G
x
A

T

M

S
x
H

W

F

Q

Q

K

E

-

E

-

G

-

G

-

V

-

I

-

K

-

R

-

G

G

T

K

K

R
|
R

H
|
H

P

P

T

T

L

V

G

G

R

D

D

Y

V

V

V

T

V

I

G

G

A

T

G

G

A

A

K

K

V

V

L

L

G

G

P

N

I

I

E

I

I

V

G

G

D

S

G

H

A

V

R

R

I

I

G

G

S

A

N

N

A

C

V

W

V

I

V

D

K

R

A

D

V

V

P

D

P

S

G

N

A

Q

T

T

A

V

binding cysteine: A208 (≠ G108), H210 (≠ S110), R224 (= R116), H225 (= H117)

4n6bA Soybean serine acetyltransferase complexed with coa (see paper)
44% identity, 60% coverage: 9:168/265 of query aligns to 62:233/233 of 4n6bA

query
sites
4n6bA

I

F

L

L

C

N

V

A

F

F

D

S

R

S

D

D

P

P

A

S

A

L

R

R

N

S

A

A

-

A

-

V

-

A

-

D

-

L

-

R

-

A

-

A

-

R

-

E

-

R

-

D

-

V

-

S

-

Y

F

S

E

H

I

C

L

L

S

L

T

N

Y

Y

P

K

G

G

L

F

H

L

A

A

V

C

M

Q

V

A

H

H

R

R

I

V

A

A

H

H

R

L

L

L

W

W

R

R

R

Q

D

S

F

R

K

R

W

P

S

L

A

A

R

L

V

A

L

L

-

H

S

S

N

R

I

I

A

A

R

N

L

V

F

F

T

A

G

-

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

R

I

I

G

G

R

K

R

G

F

I

F

L

I

F

D

D

H
|
H

G

A

M

T

G

G

V

V

V

V

I

V

G

G

E

E

T

T

A

A

V

V

I

I

G

G

D

N

D

N

C

V

T

S

L

I

Y
x
L

H

H

G

H

V

V

T

T

L

L

G

G

G
|
G

T

T

S

G

W

-

Q

-

K

-

G

G

K

D

R

R

H

H

P

P

T

K

L

I

G

G

R

D

D

G

V

V

V

L

V

I

G

G

A

A

G

G

A

A

K

T

V

I

L

L

G

G

P

N

I

I

E

K

I

I

G

G

D

E

G

G

A

A

R
x
K

I

V

G
|
G

S
x
A

N

G

A

S

V

V

V

V

V
x
L

K

I

A

D

V

V

P

P

P

P

G

R

A

T

T

T

A

A

V
|
V

G

G

V

N

P

P

G

A

R

R

I

L

I

V

binding coenzyme a: H150 (= H82), L169 (≠ Y101), G176 (= G108), K208 (≠ R143), G210 (= G145), A211 (≠ S146), L216 (≠ V151), V226 (= V161)

1sstA Serine acetyltransferase- complex with coa (see paper)
45% identity, 61% coverage: 8:168/265 of query aligns to 80:233/233 of 1sstA

query
sites
1sstA

D

D

I

I

L

Q

C

A

V

V

F

R

D

H

R

R

D

D

P

P

A

A

A

V

R

E

N

L

A

W

F

S

E

T

I

P

L

L

S

L

T

Y

Y

L

P

K

G

G

L

F

H

H

A

A

V

I

M

Q

V

S

H

Y

R

R

I

I

A

T

H

H

R

Y

L

L

W

W

R

N

R

Q

D

N

F

R

K

K

W

S

S

L

A

A

R

L

V

Y

L

L

S

Q

N

N

I

Q

A

I

R

S

L

V

F

A

T

F

G

D

I

V

E

D

I

I

H

H

P

P

G

A

A

A

V

K

I

I

G

G

R

H

R

G

F

I

F

M

I

F

D

D

H
|
H

G

A

M

T

G

G

V

I

V

V

I

V

G

G

E

E

T

T

A

S

V

V

I

I

G

E

D

N

D

D

C

V

T

S

L

I

Y
x
L

H

Q

G

G

V

V

T

T

L

L

G

G

G
|
G

T
|
T

S

-

W

-

Q

-

K

-

G

-

K

-

R

-

H

H

P

P

T

K

L

V

G

R

R

E

D

G

V

V

V

M

V

I

G

G

A
|
A

G

G

A

A

K

K

V

I

L
|
L

G

G

P

N

I

I

E

E

I

V

G

G

D

K

G

Y

A

A

R
x
K

I

I

G
|
G

S
x
A

N

N

A

S

V
|
V

V

V

V
x
L

K

N

A

P

V

V

P

P

P

E

G

Y

A

A

T
|
T

A

A

V
x
A

G

G

V
|
V

P
|
P

G

A

R

R

I

I

I
x
V

binding coenzyme a: H154 (= H82), L173 (≠ Y101), G180 (= G108), T181 (= T109), A193 (= A128), L198 (= L133), K208 (≠ R143), G210 (= G145), A211 (≠ S146), V214 (= V149), L216 (≠ V151), T224 (= T159), A226 (≠ V161), V228 (= V163), P229 (= P164), V233 (≠ I168)

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
31% identity, 38% coverage: 74:173/265 of query aligns to 77:186/201 of 1krvA

query
sites
1krvA

I

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

I

I

G

G

D

D

D

N

C

V

T

L

L

I

Y
x
A

H

P

G

N

V

V

T

T

L

L

G
x
S

G

V

T
|
T

S

G

W

H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

Q

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

R

N

D

N

V

V

V
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

P

G

I

V

E

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G

G

S
x
A

N

G

A

S

V

I

V

V

V

T

K

K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

V

V

P
|
P

G

C

R
|
R

I

V

I

I

E
x
R

P

E

E

I

R

N

D

D

binding 4-nitrophenyl beta-D-galactopyranoside: Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), M126 (≠ R116)
binding coenzyme a: A104 (≠ Y101), S110 (≠ G107), T112 (= T109), W138 (≠ V125), G140 (= G127), S141 (≠ A128), N146 (≠ L133), A159 (≠ S146), P177 (= P164), R179 (= R166), R182 (≠ E169)

Sites not aligning to the query:

binding 4-nitrophenyl beta-D-galactopyranoside: 16, 25, 70

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
31% identity, 38% coverage: 74:173/265 of query aligns to 77:186/201 of 1kruA

query
sites
1kruA

I

I

G

G

R

R

R

N

F

F

F
x
Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V
|
V

I

D

G
x
D

E

Y

T

T

A

V

V

T

I

I

G

G

D

D

D

N

C

V

T
x
L

L

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

S

G

W
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

Q

K

K

N

G

G

K

E

R
x
M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

R

N

D

N

V

V

V
x
W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

P

G

I

V

E

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G
|
G

S
x
A

N

G

A

S

V

I

V

V

V

T

K

K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

V

V

P
|
P

G

C

R

R

I

V

I

I

E
x
R

P

E

E

I

R

N

D

D

binding coenzyme a: H114 (≠ W111), W138 (≠ V125), G140 (= G127), S141 (≠ A128), G158 (= G145), A159 (≠ S146), P177 (= P164), R182 (≠ E169)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: Y82 (≠ F79), Y82 (≠ F79), N84 (≠ D81), V90 (= V87), D92 (≠ G89), L102 (≠ T99), H114 (≠ W111), M126 (≠ R116)

Sites not aligning to the query:

binding 1-methylethyl 1-thio-beta-D-galactopyranoside: 16, 22, 25, 62, 70

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
31% identity, 38% coverage: 74:173/265 of query aligns to 77:186/200 of 1krrA

query
sites
1krrA

I

I

G

G

R

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G

D

E

Y

T

T

A

V

V

T

I

I

G

G

D

D

D

N

C

V

T

L

L
x
I

Y
x
A

H
x
P

G

N

V

V

T

T

L

L

G

S

G

V

T
|
T

S

G

W
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

Q

K

K

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

R

N

D

N

V

V

V

W

V

I

G
|
G

A
x
S

G

H

A

V

K

V

V

I

L
x
N

G

P

P

G

I

V

E

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G
|
G

S
x
A

N

G

A

S

V

I

V

V

V

T

K

K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V
x
A

G

G

V

V

P
|
P

G

C

R

R

I

V

I

I

E
x
R

P

E

E

I

R

N

D

D

binding acetyl coenzyme *a: N84 (≠ D81), I103 (≠ L100), A104 (≠ Y101), P105 (≠ H102), T112 (= T109), H114 (≠ W111), G140 (= G127), S141 (≠ A128), N146 (≠ L133), G158 (= G145), A159 (≠ S146), A174 (≠ V161), P177 (= P164), R182 (≠ E169)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
31% identity, 38% coverage: 74:173/265 of query aligns to 78:187/203 of P07464

query
sites
P07464

I

I

G

G

R

R

R

N

F

F

F

Y

I

A

D
x
N

H

F

G

N

M

L

G

T

V

I

V

V

I

D

G
x
D

E

Y

T

T

A

V

V

T

I

I

G

G

D

D

D

N

C

V

T

L

L

I

Y

A

H

P

G

N

V

V

T

T

L

L

G

S

G

V

T

T

S

G

W
x
H

-

P

-

V

-

H

-

H

-

E

-

L

-

R

Q

K

K

N

G

G

K

E

R

M

H

Y

P

S

-

F

-

P

-

I

T

T

L

I

G

G

R

N

D

N

V

V

V

W

V

I

G

G

A
x
S

G

H

A

V

K

V

V

I

L

N

G

P

P

G

I

V

E

T

I

I

G

G

D

D

G

N

A

S

R

V

I

I

G

G

S
x
A

N

G

A

S

V

I

V

V

V
x
T

K
|
K

A

D

V

I

P

P

P

P

G

N

A

V

T

V

A

A

V

A

G

G

V

V

P

P

G

C

R
|
R

I

V

I

I

E
x
R

P

E

E

I

R

N

D

D

N85 (≠ D81) binding in other chain; binding in other chain
D93 (≠ G89) binding substrate
H115 (≠ W111) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ A128) binding in other chain
A160 (≠ S146) binding in other chain
TK 165:166 (≠ VK 151:152) binding acetyl-CoA
R180 (= R166) binding acetyl-CoA
R183 (≠ E169) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate
71 binding substrate

Query Sequence

>WP_012970085.1 NCBI__GCF_000025485.1:WP_012970085.1
MFNRIREDILCVFDRDPAARNAFEILSTYPGLHAVMVHRIAHRLWRRDFKWSARVLSNIA
RLFTGIEIHPGAVIGRRFFIDHGMGVVIGETAVIGDDCTLYHGVTLGGTSWQKGKRHPTL
GRDVVVGAGAKVLGPIEIGDGARIGSNAVVVKAVPPGATAVGVPGRIIEPERDATTRRRA
DTAKRIGFDAYGATRDAPDPVANAINRMLDHIHHMDQRLESMSRILEQHGLMEHFEHDGD
LDTVAIEPATQPDAARPDDPTRARG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory