SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H106) mutation to H: Little effect on the kinetic properties.
E349 (≠ D366) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
31% identity, 62% coverage: 2:391/628 of query aligns to 1:362/497 of 1ct9A

query
sites
1ct9A

C
x
A

G

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F

D

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E

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R

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L

active site: A1 (≠ C2), L50 (= L51), N74 (= N75), G75 (= G76), T305 (≠ S343), R308 (≠ M346), E332 (≠ D366)
binding adenosine monophosphate: L232 (≠ F265), L233 (= L266), S234 (= S267), S239 (= S272), A255 (≠ S293), V256 (≠ F294), D263 (≠ E301), M316 (≠ L354), S330 (= S364), G331 (= G365), E332 (≠ D366)
binding glutamine: A1 (≠ C2), R49 (= R50), L50 (= L51), I52 (= I53), V53 (≠ I54), N74 (= N75), G75 (= G76), E76 (= E77), D98 (= D100)

Sites not aligning to the query:

active site: 366

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 62% coverage: 1:389/628 of query aligns to 1:384/557 of P78753

query
sites
P78753

M

M

C

C

G

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Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
27% identity, 60% coverage: 1:375/628 of query aligns to 1:374/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

I

I

S

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G
x
A

L

L

F

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N

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Y

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Y

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V

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V

C

Y

Y

N

N

G

G

E

E

I

I

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Y

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N

F

H

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A

K

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M

A

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Q

E

H

L

F

T

E

A

F

A

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G

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H

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L

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x
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F

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G

Y

Y

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ I211) to E: in dbSNP:rs1049674
F362 (≠ L363) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
27% identity, 60% coverage: 2:375/628 of query aligns to 1:361/509 of 6gq3A

query
sites
6gq3A

C
|
C

G

G

I

I

S
x
W

G

A

L

L

F

F

D

G

T

S

R

D

E

D

R

C

R

L

A

S

V

V

D

Q

A

C

S

-

L

-

L

L

A

S

R

A

M

M

N

K

Q

-

S

-

L

I

F

A

H

H

R

R

G

G

P

P

D

D

E

A

G

F

G

R

Q

F

H

E

T

N

E

V

P

N

G

G

-

Y

-

T

-

N

V

C

G

C

L

F

A

G

H

F

R

H

R
|
R

L
|
L

S

A

I
x
V

D

D

L

P

A

L

S

F

G

G

Q

M

Q

Q

P

P

L

I

-

R

F

V

N

K

E

K

D

Y

Q

P

T

Y

V

L

V

W

V

L

V

C

Y
|
Y

N
|
N

G
|
G

E
|
E

I

I

Y

Y

N

N

F

H

Q

K

A

K

L

M

A

Q

H

Q

E

H

L

F

T

E

A

F

A

E

G

-

H

-

V

-

F

Y

R

Q

T

T

H

K

C
x
V

D
|
D

T

G

E

E

V

I

I

I

V

L

H

H

A

L

W

Y

E

D

Q

K

W

G

G

G

-

I

E

E

G

Q

C

T

V

I

E

C

R

M

F

L

R

D

G

G

M

V

F

F

A

A

F

F

A

V

L

L

W

L

D

D

R

T

N

A

R

N

E

K

T

K

L

V

F

F

L

L

A

G

R

R

D

D

R

T

L

Y

G

G

I

V

K

R

P

P

L

L

H

F

Y

K

A

A

W

M

R

T

P

E

D

D

G

G

Q

F

L

L

L

A

F

V

G

C

S

S

E

E

I

A

K

K

A

G

L

L

L

V

E

-

D

-

A

-

S

T

L

L

S

K

R

H

E

S

I

A

D

T

P

P

L

F

A

L

V

K

E

V

E

E

Y

P

F

F

A

L

Y

P

G

G

Y

H

V

Y

P

E

-

V

-

L

-

D

-

L

D

K

P

P

R

N

S

G

I

K

F

V

K

A

S

S

I

V

H

E

K

M

L

V

P

K

P

Y

G

H

H

H

T

C

L

R

T

D

I

V

R

F

R

P

G

G

V

-

N

-

Q

-

R

-

P

-

E

-

P

-

R

-

E

-

Y

-

W

F

D

E

V

I

S

E

F

T

R

V

P

K

N

N

G

N

V

L

Q

R

D

I

V

L

A

-

E

-

A

-

T

-

A

-

E

-

V

-

I

-

E

-

R

-

L

F

R

N

E

N

A

A

V

V

N

K

I

K

R

R

L

L

V

M

A

T

E

D

V

R

P

R

L

I

G

G

A

C

F

L

L

L

S

S

G

G

V

L

D

D

S

S

A

L

V

V

V

A

A

A

M

T

M

L

A

L

G

K

L

Q

S

L

D

K

Q

E

-

A

-

Q

-

V

-

Q

-

Y

P

P

V

L

N

Q

T

T

C

F

S

A

I

I

S

G

F

M

G

E

D

D

P

S

A

P

F

-

N

-

E

D

S

L

A

L

Y

A

A

A

E

R

Q

K

V

V

A

A

R

D

R

H

Y

I

A

G

T

S

N

E

H

H

H

Y

-

E

-

V

-

L

-

F

-

N

-

S

V

E

E

E

S

G

V

I

E

Q

P

A

D

L

D

D

F

-

E

E

L

V

I

I

E

F

R

S

L

L

A

E

G

-

I

T

Y

Y

D

D

E

I

P
x
T

Y

T

A

V

D
x
R

S

A

S

S

A

-

M

V

P

G

T

M

Y

Y

R

L

V

I

C

S

E

K

L

Y

A

I

R

R

K

K

R

N

V

T

-

D

-

S

T

V

V

V

C

I

L

F

S

S

G

G

D

E

G

G

G

S

D

D

E

E

S

L

F

T

A

Q

G

G

Y

Y

active site: W4 (≠ S5), L49 (= L51), N74 (= N75), G75 (= G76), T324 (≠ P339), R327 (≠ D342)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R50), V51 (≠ I53), V52 (≠ I54), Y73 (= Y74), N74 (= N75), G75 (= G76), E76 (= E77), V95 (≠ C99), D96 (= D100)

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
30% identity, 18% coverage: 69:178/628 of query aligns to 96:212/460 of 6lbpA

query
sites
6lbpA

T

S

V

V

V

G

V

V

V

A

Y

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

F

Y

Q

T

A

K

L

L

A

R

H

A

E

D

L

L

T

E

A

E

A

N

G

G

H

S

V

I

F

F

R

N

T

T

H

S

C

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

A

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

W

F

E

M

Q

R

W

I

G

V

E

D

G

A

C

C

V

-

E

E

R

K

F

L

R

Q

G

G

M

A

F

Y

A

S

F

M

A

V

L

F

W

V

D

T

R

E

N

D

R

K

E

-

T

-

L

L

F

V

L

A

A

V

R

R

D

D

R

P

L

H

G

G

I

F

K

R

P

P

L

L

H

V

Y

M

A

G

W

R

R

R

P

S

D

N

G

G

Q

A

L

V

F

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

-

D

L

L

E

I

D

E

A

A

S

T

L

Y

S

E

R

R

E

E

I

V

D

Y

P

P

active site: N102 (= N75), G103 (= G76)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
30% identity, 18% coverage: 69:178/628 of query aligns to 182:298/561 of Q9STG9

query
sites
Q9STG9

T

S

V

V

V

G

V

V

V

A

Y
x
H

N

N

G

G

E

N

I

L

Y

V

N

N

F

Y

Q

T

A

K

L

L

A

R

H

A

E

D

L

L

T

E

A

E

A

N

G

G

H

S

V

I

F

F

R

N

T

T

H

S

C

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

A

L

-

I

-

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

W

F

E

M

Q

R

W

I

G

V

E

D

G

A

C

C

V

-

E

E

R

K

F

L

R

Q

G

G

M

A

F

Y

A

S

F

M

A

V

L

F

W

V

D

T

R

E

N

D

R

K

E

-

T

-

L

L

F

V

L

A

A

V

R

R

D

D

R

P

L

H

G

G

I

F

K
x
R

P
|
P

L

L

H

V

Y

M

A

G

W

R

R

R

P

S

D

N

G

G

Q

A

L

V

F

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

-

D

L

L

E

I

D

E

A

A

S

T

L

Y

S

E

R

R

E

E

I

V

D

Y

P

P

H187 (≠ Y74) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K145) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P146) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
33% identity, 15% coverage: 43:139/628 of query aligns to 66:173/608 of 4amvA

query
sites
4amvA

G

G

V

T

G

G

L

I

A

A

H

H

R

T

R

R

L
x
W

S

A

I

T

I

H

D

G

L

E

A

P

S

S

G

E

Q

V

Q

N

P

A

L

H

F

P

N

H

E

V

D

S

Q

E

T

H

V

I

V

V

Y

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

F

H

Q

E

A

P

L

L

A

R

H

E

E

E

L

L

T

K

A

A

A

R

G

G

H

Y

V

T

F

F

R

V

T

S

H

E

C

T

D

D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

Q

L

W

K

G

Q

E

G

G

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

-

R

C

A

V

I

E

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

active site: W74 (≠ L51), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding fructose -6-phosphate: 301, 302, 303, 347, 348, 349, 352, 401, 485, 488

1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
33% identity, 15% coverage: 43:139/628 of query aligns to 66:173/608 of 1jxaA

query
sites
1jxaA

G

G

V

T

G

G

L

I

A

A

H

H

R

T

R

R

L
x
W

S

A

I

T

I

H

D

G

L

E

A

P

S

S

G

E

Q

V

Q

N

P

A

L

H

F

P

N

H

E

V

D

S

Q

E

T

H

V

I

V

V

Y

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

F

H

Q

E

A

P

L

L

A

R

H

E

E

E

L

L

T

K

A

A

A

R

G

G

H

Y

V

T

F

F

R

V

T

S

H

E

C

T

D

D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

Q

L

W

K

G

Q

E

G

G

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

-

R

C

A

V

I

E

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

active site: W74 (≠ L51), N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 303, 347, 348, 349, 352, 401, 485, 488

2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
33% identity, 15% coverage: 43:139/628 of query aligns to 66:173/608 of 2j6hA

query
sites
2j6hA

G

G

V

T

G

G

L

I

A

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

D

G

L

E

A

P

S

S

G

E

Q

V

Q

N

P

A

L
x
H

F

P

N

H

E

V

D

S

Q

E

T

H

V

I

V

V

Y

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

F

H

Q

E

A

P

L

L

A

R

H

E

E

E

L

L

T

K

A

A

A

R

G

G

H

Y

V

T

F

F

R

V

T

S

H

E

C

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

Q

L

W

K

G

Q

E

G

G

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

-

R

C

A

V

I

E

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

active site: W74 (≠ L51), N98 (= N75), G99 (= G76)
binding 5-oxo-l-norleucine: R73 (= R50), W74 (≠ L51), T76 (≠ I53), H86 (≠ L63), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27, 248, 481, 485, 488, 504, 603
binding glucose-6-phosphate: 302, 347, 348, 349, 352, 399, 401, 488
binding 5-oxo-l-norleucine: 1

1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
33% identity, 15% coverage: 43:139/628 of query aligns to 66:173/238 of 1xfgA

query
sites
1xfgA

G

G

V

T

G

G

L

I

A

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

D

G

L

E

A

P

S

S

G

E

Q

V

Q

N

P

A

L
x
H

F

P

N

H

E

V

D

S

Q

E

T

H

V

I

V

V

Y

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

F

H

Q

E

A

P

L

L

A

R

H

E

E

E

L

L

T

K

A

A

A

R

G

G

H

Y

V

T

F

F

R

V

T

S

H

E

C

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

Q

L

W

K

G

Q

E

G

G

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

-

R

C

A

V

I

E

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

active site: W74 (≠ L51), N98 (= N75), G99 (= G76)
binding glutamine hydroxamate: R73 (= R50), W74 (≠ L51), T76 (≠ I53), H86 (≠ L63), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27
binding glutamine hydroxamate: 1

1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
33% identity, 15% coverage: 43:139/628 of query aligns to 66:173/238 of 1xffA

query
sites
1xffA

G

G

V

T

G

G

L

I

A

A

H

H

R

T

R
|
R

L
x
W

S

A

I
x
T

I

H

D

G

L

E

A

P

S

S

G

E

Q

V

Q

N

P

A

L
x
H

F

P

N

H

E

V

D

S

Q

E

T

H

V

I

V

V

Y

H

N
|
N

G
|
G

E

I

I

I

Y

E

N

N

F

H

Q

E

A

P

L

L

A

R

H

E

E

E

L

L

T

K

A

A

A

R

G

G

H

Y

V

T

F

F

R

V

T

S

H

E

C

T

D
|
D

T

T

E

E

V

V

I

I

V

A

H

H

-

L

-

V

A

N

W

W

E

E

Q

L

W

K

G

Q

E

G

G

G

-

T

-

L

-

R

-

E

-

A

-

V

-

L

-

R

C

A

V

I

E

P

R

Q

F

L

R

R

G

G

M

A

F

Y

A

G

F

T

A

V

L

I

W

M

D

D

-

S

R

R

N

H

R

P

E

D

T

T

L

L

F

L

L

A

A

A

R

R

active site: W74 (≠ L51), N98 (= N75), G99 (= G76)
binding glutamic acid: R73 (= R50), W74 (≠ L51), T76 (≠ I53), H86 (≠ L63), N98 (= N75), G99 (= G76), D123 (= D100)

Sites not aligning to the query:

active site: 1, 26, 27
binding glutamic acid: 1

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
24% identity, 19% coverage: 61:178/628 of query aligns to 81:207/455 of 1ao0A

query
sites
1ao0A

Q

Q

P

P

L

L

F

L

N

F

E

R

D

S

Q

Q

-

N

-

G

T

S

V

L

V

A

V

L

V

A

Y

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

F

A

Q

T

A

Q

L

L

A

K

H

Q

E

Q

L

L

T

E

A

N

A

Q

G

G

H

S

V

I

F

F

R

Q

T

T

H

S

C

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

W

I

E

K

Q

R

W

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

E

K

G

N

C

S

V

L

E

S

R

M

F

L

R

K

G

G

M

A

F

Y

A

A

F

F

A

L

L

I

W

M

D

T

R

E

N

T

R

E

E

-

T

-

L

M

F

I

L

V

A

A

R

L

D

D

R

P

L

N

G

G

I

L

K

R

P

P

L

L

H

S

Y

I

A

G

W

M

R

M

P

G

D

D

G

A

Q

Y

L

V

F

A

G

S

S

E

E

T

I

C

K

A

A

F

L

D

L

V

E

V

D

G

A

A

S

T

L

Y

S

L

R

R

E

E

I

V

D

E

P

P

active site: N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 27, 238, 296, 301, 311, 419
binding guanosine-5'-monophosphate: 234, 238, 279, 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 25, 238, 240, 241, 242, 277, 278, 279, 300, 301, 301, 303
binding magnesium ion: 279, 341, 342
binding iron/sulfur cluster: 232, 233, 234, 378, 380, 433, 436

P00497 Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase; EC 2.4.2.14 from Bacillus subtilis (strain 168) (see 5 papers)
24% identity, 19% coverage: 61:178/628 of query aligns to 96:222/476 of P00497

query
sites
P00497

Q

Q

P

P

L

L

F

L

N

F

E

R

D

S

Q

Q

-

N

-

G

T

S

V

L

V

A

V

L

V

A

Y

H

N

N

G

G

E

N

I

L

Y

V

N

N

F

A

Q

T

A

Q

L

L

A

K

H

Q

E

Q

L

L

T

E

A

N

A

Q

G

G

H

S

V

I

F

F

R

Q

T

T

H

S

C

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

W

I

E

K

Q

R

W

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

E

K

G

N

C

S

V

L

E

S

R

M

F

L

R

K

G

G

M

A

F

Y

A

A

F

F

A

L

L

I

W

M

D

T

R

E

N

T

R

E

E

-

T

-

L

M

F

I

L

V

A

A

R

L

D

D

R

P

L

N

G

G

I

L

K

R

P

P

L

L

H

S

Y

I

A

G

W

M

R

M

P

G

D

D

G

A

Q

Y

L

V

F

A

G

S

S

E

E

T

I

C

K

A

A

F

L

D

L

V

E

V

D

G

A

A

S

T

L

Y

S

L

R

R

E

E

I

V

D

E

P

P

Sites not aligning to the query:

1:11 modified: propeptide
12 active site, Nucleophile; C→F: Loss of enzyme activity and N-terminal processing.
247 binding [4Fe-4S] cluster
294 binding Mg(2+)
356 binding Mg(2+)
357 binding Mg(2+)
393 binding [4Fe-4S] cluster
394 F→V: Partial loss of activity.
442 D→S: Partial loss of activity.
448 binding [4Fe-4S] cluster; C→S: Loss of activity.
451 binding [4Fe-4S] cluster; C→S: Loss of activity.
452 F→C: Lethal.

1gph1 Structure of the allosteric regulatory enzyme of purine biosynthesis (see paper)
24% identity, 19% coverage: 61:178/628 of query aligns to 85:211/465 of 1gph1

query
sites
1gph1

Q

Q

P

P

L

L

F

L

N

F

E

R

D

S

Q

Q

-

N

-

G

T

S

V

L

V

A

V

L

V

A

Y

H

N

N

G

G

E

N

I

L

Y

V

N

N

F

A

Q

T

A

Q

L

L

A

K

H

Q

E

Q

L

L

T

E

A

N

A

Q

G

G

H

S

V

I

F

F

R

Q

T

T

H

S

C

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

W

I

E

K

Q

R

W

S

G

G

-

H

-

F

-

T

-

L

-

K

-

D

-

Q

-

I

E

K

G

N

C

S

V

L

E

S

R

M

F

L

R

K

G

G

M

A

F

Y

A

A

F

F

A

L

L

I

W

M

D

T

R

E

N

T

R

E

E

-

T

-

L

M

F

I

L

V

A

A

R

L

D

D

R

P

L

N

G

G

I

L

K

R

P

P

L

L

H

S

Y

I

A

G

W

M

R

M

P

G

D

D

G

A

Q

Y

L

V

F

A

G

S

S

E

E

T

I

C

K

A

A

F

L

D

L

V

E

V

D

G

A

A

S

T

L

Y

S

L

R

R

E

E

I

V

D

E

P

P

Sites not aligning to the query:

active site: 300, 305, 315, 423
binding adenosine monophosphate: 242, 242, 244, 245, 246, 282, 283, 283, 304, 305, 307, 345, 346, 347, 349, 350, 353, 388
binding iron/sulfur cluster: 236, 237, 382, 384, 388, 437, 440

1jgtB Crystal structure of beta-lactam synthetase (see paper)
26% identity, 43% coverage: 70:336/628 of query aligns to 68:331/500 of 1jgtB

query
sites
1jgtB

V

T

V

A

V

V

Y

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

Q

D

A

E

L

L

A

L

H

S

E

V

L

L

T

P

A

A

A

G

G

P

H

-

V

-

F

-

R

A

T

P

H

E

C

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

W

L

E

E

Q

R

W

Y

G

D

E

L

G

H

C

A

V

F

E

R

R

L

F

V

R

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

-

D

-

R

R

N

T

R

G

E

D

T

R

L

V

F

L

L

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

H

Y

Y

T

A

C

W

V

R

A

P

P

D

-

G

-

Q

-

L

-

F

-

G

-

S

G

E

E

I

V

K

R

A

A

L

S

L

T

E

E

D

A

A

K

S

A

L

L

S

A

R

A

E

H

I

R

D

D

P

P

L

-

A

-

V

-

E

-

Y

-

F

-

A

-

Y

K

G

G

Y

F

-

P

V

L

P

A

D

D

P

A

R

R

S

R

I

V

-

A

-

G

F

L

K

T

S

G

I

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

T

V

L

M

T

D

I

I

R

D

R

L

G

G

V

S

N

G

Q

T

R

A

P

V

E

T

P

H

R

R

E

T

Y

W

W

T

D

P

V

G

S

L

F

S

R

R

-

R

-

I

-

L

P

P

N

E

G

G

V

-

Q

-

D

-

V

-

A

-

E

E

A

A

T

V

A

A

E

A

V

V

I

R

E

A

R

A

L

L

R

E

E

K

A

A

V

V

N

A

I

Q

R

R

L

V

V

T

-

P

A

G

E

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V
x
I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

A

A

M

C

-

A

-

H

-

R

M

A

A

A

G

G

L

E

S

L

D

D

Q

-

P

-

V

-

N

-

T

-

C

-

S

T

I

V

S
|
S

F
x
M

G

G

D

T

P

D

A

T

F

S

N

N

E

E

S

F

A

R

Y

E

A

A

E

R

Q

A

V

V

A

V

R

D

R

H

Y

L

A

R

T

T

N

R

H

H

H

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

-

Q

-

L

-

P

-

Y

-

A

-

V

-

W

-

A

V

S

E

E

S

S

V

V

E

D

P

P

D
|
D

D

I

F

I

E

E

L
x
Y

I

L

E

L

R

P

L
|
L

A

T

G

A

I

L

Y

Y

active site: A73 (≠ N75), G74 (= G76), D319 (= D324)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F265), L245 (= L266), S246 (= S267), G248 (= G269), I249 (≠ V270), D250 (= D271), S251 (= S272), S269 (= S293), M270 (≠ F294), L327 (= L332)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ L328)
binding magnesium ion: D250 (= D271)

Sites not aligning to the query:

active site: 345, 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 344, 345, 348, 420, 440
binding n2-(carboxyethyl)-l-arginine: 345, 346, 348, 349, 354, 370, 379
binding magnesium ion: 348

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
26% identity, 43% coverage: 70:336/628 of query aligns to 64:323/496 of 1mbzA

query
sites
1mbzA

V

T

V

A

V

V

Y

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

Q

D

A

E

L

L

A

L

H

S

E

V

L

L

T

P

A

A

A

G

G

P

H

-

V

-

F

-

R

A

T

P

H

E

C

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

W

L

E

E

Q

R

W

Y

G

D

E

L

G

H

C

A

V

F

E

R

R

L

F

V

R

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

-

D

-

R

R

N

T

R

G

E

D

T

R

L

V

F

L

L

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

H

Y

Y

T

A

C

W

V

R

A

P

P

D

-

G

-

Q

-

L

-

F

-

G

-

S

G

E

E

I

V

K

R

A

A

L

S

L

T

E

E

D

A

A

K

S

A

L

L

S

A

R

A

E

H

I

G

D

F

P

P

L

L

A

A

V

-

E

-

Y

-

F

-

A

-

Y

-

G

-

Y

-

V

-

P

-

D

D

P

A

R

R

S

R

I

V

-

A

-

G

F

L

K

T

S

G

I

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

T

V

L

M

T

D

I

I

R

D

R

L

G

G

V

S

N

G

Q

T

R

A

P

V

E

T

P

H

R

R

E

T

Y

W

W

T

D

P

V

G

S

L

F

S

R

R

-

R

-

I

-

L

P

P

N

E

G

G

V

-

Q

-

D

-

V

-

A

-

E

E

A

A

T

V

A

A

E

A

V

V

I

R

E

A

R

A

L

L

R

E

E

K

A

A

V

V

N

A

I

Q

R

R

L

V

V

T

-

P

A

G

E

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

A

A

M

C

-

A

-

H

-

R

M

A

A

A

G

G

L

E

S

L

D

D

Q

-

P

-

V

-

N

-

T

-

C

-

S

T

I

V

S
|
S

F
x
M

G

G

D

T

P

D

A

T

F

S

N

N

E

E

S

F

A

R

Y

E

A

A

E

R

Q

A

V

V

A

V

R

D

R

H

Y

L

A

R

T

T

N

R

H

H

H

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

-

Q

-

L

-

P

-

Y

-

A

-

V

-

W

-

A

V

S

E

E

S

S

V

V

E

D

P

P

D
|
D

D

I

F

I

E

E

L
x
Y

I

L

E

L

R

P

L
|
L

A

T

G

A

I

L

Y

Y

active site: A69 (≠ N75), G70 (= G76), D311 (= D324)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F265), L237 (= L266), S238 (= S267), S243 (= S272), S261 (= S293), M262 (≠ F294), Y315 (≠ L328), L319 (= L332)
binding magnesium ion: D242 (= D271)
binding pyrophosphate 2-: S238 (= S267), G240 (= G269), D242 (= D271), S243 (= S272)

Sites not aligning to the query:

active site: 337, 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 336, 337, 338, 340, 341, 362, 371, 432, 434, 435
binding magnesium ion: 340
binding pyrophosphate 2-: 340, 412, 432, 433

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
26% identity, 43% coverage: 70:336/628 of query aligns to 60:318/491 of 1mc1A

query
sites
1mc1A

V

T

V

A

V

V

Y

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

Q

D

A

E

L

L

A

L

H

S

E

V

L

L

T

P

A

A

A

G

G

P

H

-

V

-

F

-

R

A

T

P

H

E

C

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

W

L

E

E

Q

R

W

Y

G

D

E

L

G

H

C

A

V

F

E

R

R

L

F

V

R

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

-

D

-

R

R

N

T

R

G

E

D

T

R

L

V

F

L

L

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

H

Y

Y

T

A

C

W

V

R

A

P

P

D

-

G

G

Q

E

L

V

L

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

L

-

E

-

D

-

A

-

S

-

L

-

S

-

R

-

E

-

I

-

D

-

P

-

L

-

A

-

V

-

E

-

Y

-

F

-

A

A

Y

A

G

G

Y

F

-

P

V

L

P

A

D

D

P

A

R

R

S

R

I

V

-

A

-

G

F

L

K

T

S

G

I

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

T

V

L

M

T

D

I

I

R

D

R

L

G

G

V

S

N

G

Q

T

R

A

P

V

E

T

P

H

R

R

E

T

Y

W

W

T

D

P

V

G

S

L

F

S

R

R

-

R

-

I

-

L

P

P

N

E

G

G

V

-

Q

-

D

-

V

-

A

-

E

E

A

A

T

V

A

A

E

A

V

V

I

R

E

A

R

A

L

L

R

E

E

K

A

A

V

V

N

A

I

Q

R

R

L

V

V

T

-

P

A

G

E

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L

L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

A

A

M

C

-

A

-

H

-

R

M

A

A

A

G

G

L

E

S

L

D

D

Q

-

P

-

V

-

N

-

T

-

C

-

S

T

I

V

S
|
S

F
x
M

G

G

D

T

P

D

A

T

F

S

N

N

E

E

S

F

A

R

Y

E

A

A

E

R

Q

A

V

V

A

V

R

D

R

H

Y

L

A

R

T

T

N

R

H

H

H

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

-

Q

-

L

-

P

-

Y

-

A

-

V

-

W

-

A

V

S

E

E

S

S

V

V

E

D

P

P

D
|
D

D

I

F

I

E

E

L
x
Y

I

L

E

L

R

P

L

L

A

T

G

A

I

L

Y

Y

active site: A65 (≠ N75), G66 (= G76), D306 (= D324)
binding adenosine monophosphate: V231 (≠ F265), S233 (= S267), S238 (= S272), S256 (= S293), M257 (≠ F294)
binding magnesium ion: D237 (= D271)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ L328)
binding pyrophosphate 2-: S233 (= S267), G235 (= G269), D237 (= D271), S238 (= S272)

Sites not aligning to the query:

active site: 332, 366, 427
binding adenosine monophosphate: 331, 427, 430
binding magnesium ion: 335
binding deoxyguanidinoproclavaminic acid: 332, 333, 336, 357, 366, 427
binding pyrophosphate 2-: 335, 407, 427, 428

1mb9A Beta-lactam synthetase complexed with atp (see paper)
27% identity, 43% coverage: 70:336/628 of query aligns to 65:322/485 of 1mb9A

query
sites
1mb9A

V

T

V

A

V

V

Y

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

Q

D

A

E

L

L

A

L

H

S

E

V

L

L

T

P

A

A

A

G

G

P

H

A

V

-

F

-

R

-

T

P

H

E

C

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

W

L

E

E

Q

R

W

Y

G

D

E

L

G

H

C

A

V

F

E

R

R

L

F

V

R

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

-

D

-

R

R

N

T

R

G

E

D

T

R

L

V

F

L

L

L

A

A

R

T

D

D

R

H

L

A

G

G

I

S

K

V

P

P

L

L

H

Y

Y

T

A

C

W

V

R

A

P

P

D

-

G

G

Q

E

L

V

L

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

L

-

E

-

D

-

A

-

S

-

L

-

S

-

R

-

E

A

I

A

D

F

P

P

L

L

A

A

V

-

E

-

Y

-

F

-

A

-

Y

-

G

-

Y

-

V

-

P

-

D

D

P

A

R

R

S

R

I

V

-

A

-

G

F

L

K

T

S

G

I

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

T

V

L

M

T

D

I

I

R

D

R

L

G

G

V

S

N

G

Q

T

R

A

P

V

E

T

P

H

R

R

E

T

Y

W

W

T

D

P

V

G

S

L

F

S

R

R

-

R

-

I

-

L

P

P

N

E

G

G

V

-

Q

-

D

-

V

-

A

-

E

E

A

A

T

V

A

A

E

A

V

V

I

R

E

A

R

A

L

L

R

E

E

K

A

A

V

V

N

A

I

Q

R

R

L

V

V

T

-

P

A

G

E

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

A

A

M

C

-

A

-

H

-

R

M

A

A

A

G

G

L

E

S

L

D

D

Q

-

P

-

V

-

N

-

T

-

C

-

S

T

I

V

S
|
S

F
x
M

G

G

D

T

P

D

A

T

F

S

N

N

E

E

S

F

A

R

Y

E

A

A

E

R

Q

A

V

V

A

V

R

D

R

H

Y

L

A

R

T

T

N

R

H

H

H

R

-

E

-

I

-

T

-

I

-

P

-

T

-

E

-

L

-

A

-

Q

-

L

-

P

-

Y

-

A

-

V

-

W

-

A

V

S

E

E

S

S

V

V

E

D

P

P

D
|
D

D

I

F

I

E

E

L
x
Y

I

L

E

L

R

P

L
|
L

A

T

G

A

I

L

Y

Y

active site: A70 (≠ N75), G71 (= G76), D310 (= D324)
binding adenosine monophosphate: V235 (≠ F265), L236 (= L266), S242 (= S272), S260 (= S293), M261 (≠ F294), Y314 (≠ L328), L318 (= L332)
binding adenosine-5'-triphosphate: V235 (≠ F265), L236 (= L266), S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272), S260 (= S293), M261 (≠ F294), L318 (= L332)
binding magnesium ion: D241 (= D271)
binding pyrophosphate 2-: S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272)

Sites not aligning to the query:

active site: 336, 370, 431
binding adenosine monophosphate: 335, 336
binding adenosine-5'-triphosphate: 335, 339, 411, 431
binding magnesium ion: 339
binding pyrophosphate 2-: 339, 411, 431

Query Sequence

>WP_012970981.1 NCBI__GCF_000025485.1:WP_012970981.1
MCGISGLFDTRERRAVDASLLARMNQSLFHRGPDEGGQHTEPGVGLAHRRLSIIDLASGQ
QPLFNEDQTVVVVYNGEIYNFQALAHELTAAGHVFRTHCDTEVIVHAWEQWGEGCVERFR
GMFAFALWDRNRETLFLARDRLGIKPLHYAWRPDGQLLFGSEIKALLEDASLSREIDPLA
VEEYFAYGYVPDPRSIFKSIHKLPPGHTLTIRRGVNQRPEPREYWDVSFRPNGVQDVAEA
TAEVIERLREAVNIRLVAEVPLGAFLSGGVDSSAVVAMMAGLSDQPVNTCSISFGDPAFN
ESAYAEQVARRYATNHHVESVEPDDFELIERLAGIYDEPYADSSAMPTYRVCELARKRVT
VCLSGDGGDESFAGYRRYRWHLHEERVRRRLPQAVRGPVFGLLGALYPKADWAPKVLRAK
STLQALALDSVEGYFESVSILTDGVRRDLFSARLKRELQGYRAVEVLRRHAARAPEHPLS
RVQYLDMKTYLPGDILTKVDRASMAHALEVRVPILDHLFLDWVSGLSPDLKYAGGEGKWI
LKKALEPYLGPETLYRPKMGFAVPLTSWFRGPLRERVRQLLLGAPLLDSGFFDERQVRWI
VDQHQSGMRDFSPAIWSLMMFAAFNARL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory