SitesBLAST

P0A6F1 Carbamoyl phosphate synthase small chain; Carbamoyl phosphate synthetase glutamine chain; EC 6.3.5.5 from Escherichia coli (strain K12) (see paper)
54% identity, 97% coverage: 12:388/390 of query aligns to 2:378/382 of P0A6F1

query
sites
P0A6F1

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S47 (= S57) binding L-glutamine
G241 (= G250) binding L-glutamine
G243 (= G252) binding L-glutamine
L270 (= L280) binding L-glutamine
Q273 (= Q283) binding L-glutamine
N311 (= N321) binding L-glutamine
G313 (= G323) binding L-glutamine
F314 (= F324) binding L-glutamine

1c3oB Crystal structure of the carbamoyl phosphate synthetase: small subunit mutant c269s with bound glutamine (see paper)
54% identity, 97% coverage: 12:388/390 of query aligns to 1:377/379 of 1c3oB

query
sites
1c3oB

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active site: K201 (= K211), S268 (≠ C279), H352 (= H363), E354 (= E365)
binding glutamine: S46 (= S57), N239 (= N249), G240 (= G250), G242 (= G252), S268 (≠ C279), L269 (= L280), N310 (= N321), H311 (= H322), G312 (= G323), F313 (= F324)

Q9LVW7 Carbamoyl phosphate synthase small chain, chloroplastic; Carbamoyl phosphate synthetase glutamine chain; Protein VENOSA 6; EC 6.3.5.5 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
46% identity, 96% coverage: 17:390/390 of query aligns to 60:429/430 of Q9LVW7

query
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Q9LVW7

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H410 (≠ Q371) mutation to Y: In ven6-1; reticulate leaf phenotype.

P27708 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Homo sapiens (Human) (see 7 papers)
41% identity, 96% coverage: 17:389/390 of query aligns to 4:362/2225 of P27708

query
sites
P27708

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R

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A

M33 (= M46) to R: in DEE50; uncertain significance; dbSNP:rs751610198
R177 (= R200) to Q: in a colorectal cancer sample; somatic mutation; dbSNP:rs374122292

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
456 modified: Phosphothreonine; by MAPK1
735 Y → C: in a colorectal cancer sample; somatic mutation
1406 modified: Phosphoserine; by PKA
1471 binding Zn(2+); binding Zn(2+); H→A: No zinc-binding and no catalytic activity.; H→N: Abolishes dihydroorotase activity.
1473 binding Zn(2+); H→A: No zinc-binding and no catalytic activity.
1475 binding (S)-dihydroorotate
1505 binding (S)-dihydroorotate
1512 D→N: No change in catalytic activity.
1556 binding via carbamate group; binding via carbamate group; modified: N6-carboxylysine
1562 T→A: Abolishes dihydroorotase activity.
1563 F→A: Abolishes dihydroorotase activity.
1590 binding Zn(2+); H→A: Abolishes dihydroorotase activity.; H→N: No catalytic activity.
1613 binding Zn(2+); C→S: Reduces dihydroorotase activity.
1614 binding Zn(2+); H→A: Abolishes dihydroorotase activity.
1637 binding Zn(2+); E→T: Abolishes dihydroorotase activity.
1642 H→N: 11.5% of wild-type catalytic activity.
1661 binding (S)-dihydroorotate
1686 binding Zn(2+); D→N: Abolishes dihydroorotase activity.
1690 binding (S)-dihydroorotate; H→N: 3% of wild-type catalytic activity.
1702 binding (S)-dihydroorotate
1789:2225 natural variant: Missing (in DEE50; uncertain significance)
1859 modified: Phosphoserine; by RPS6KB1 and PKA
1873 modified: Phosphoserine; by PKC; in vitro; S→A: Abolishes PMA-induced Thr-456 phosphorylation.
1900 modified: Phosphoserine

P20054 Multifunctional protein pyr1-3; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Dictyostelium discoideum (Social amoeba)
39% identity, 94% coverage: 15:381/390 of query aligns to 9:379/2225 of P20054

query
sites
P20054

G

G

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C

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L

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L

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L

L

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N

F

F

V

V

Sites not aligning to the query:

1 modified: N-acetylmethionine

Q18990 Multifunctional protein pyr-1; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Caenorhabditis elegans (see 3 papers)
38% identity, 96% coverage: 17:389/390 of query aligns to 5:365/2198 of Q18990

query
sites
Q18990

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N

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A

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N

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Q

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N

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A

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Sites not aligning to the query:

1602 H→Q: In cu8; probable loss of dihydroorotase activity. Severe late stage embryonic lethality in 50% of animals. The few surviving mutants have a shorter and thicker pharyngeal isthmus, an abnormal knobbed tail, mild egg-laying defects, moderate fluid accumulation in the coelom and a slower growth. Actin and intermediate filaments are disorganized in the pharynx. Moderate reduction in heparan sulfate levels and increased levels of chondroitin sulfate. In an umps-1 zu456 mutant background, prevents the formation of abnormally enlarged gut granules in embryos. Complete embryonic lethality in a rnst-2 qx245 mutant background.

P08955 Multifunctional protein CAD; Carbamoyl phosphate synthetase 2-aspartate transcarbamylase-dihydroorotase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Mesocricetus auratus (Golden hamster) (see paper)
41% identity, 96% coverage: 17:389/390 of query aligns to 4:362/2225 of P08955

query
sites
P08955

L

L

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V

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A

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N

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G

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L

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T

I

L

D

P

A

F

L

V

V

D

A

P

K

N

A

A

K

R

G

-

W

-

P

-

G

-

L

-

E

-

G

-

M

-

D

P

L

L

A

A

K

P

D

E

V

V

S

S

C

I

R

K

Q

-

T

-

Y

-

D

-

W

-

T

T

E

P

A

R

A

V

W

F

T

N

I

A

G

G

G

A

Y

-

A

-

T

-

Q

-

D

-

K

-

P

P

R

R

Y

-

H

-

V

I

V

C

A

A

I

L

D

D

Y

C

G

G

A

L

K

K

R

Y

N

N

I

Q

L

I

R

R

C

C

L

L

A

C

A

Q

A

L

G

G

C

A

K

E

V

V

T

T

V

V

P

P

S

W

T

N

A

H

S

E

V

L

E

D

D

S

V

-

M

-

R

-

H

Q

Q

K

P

Y

D

D

G

G

V

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

A

A

S

T

Y

G

P

E

G

Y

-

A

V

V

V

P

A

T

T

I

L

K

N

G

R

L

V

L

L

D

S

-

E

-

P

T

N

G

P

V

R

P

P

M

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

L

S

A

L

L

A

A

L

I

G

G

A

A

K

K

T

T

T

Y

K

K

M

M

Q

R

Q

Y

G

G

H

N

R

R

G

G

A

H

N

N

H

Q

P

P

V

C

K

L

D

L

L

V

V

G

S

T

G

G

R

R

V

C

E

F

I

L

T

T

S

S

Q

Q

N

N

H

H

G

G

F

F

V

A

V

V

V

D

P

A

E

D

T

S

L

L

P

P

A

A

D

G

A

W

E

T

V

P

T

L

H

F

V

T

S

N

L

A

F

N

D

D

G

C

T

S

N

N

E

E

G

G

I

I

R

V

L

H

K

D

G

S

K

L

P

P

V

F

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

H

S

R

P

A

G

G

P

P

Q

S

D

D

S

M

H

E

Y

L

L

L

F

F

D

D

R

V

F

F

V

L

Q

E

L

T

I

V

A

R

E

E

K

A

K

V

A

A

Sites not aligning to the query:

1406 modified: Phosphoserine; by PKA; S→A: No effect on enzyme kinetics.; S→D: Increases CPSase activity and reduces sensitivity to feedback inhibition by UTP.

P07259 Multifunctional protein URA2; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
36% identity, 98% coverage: 5:388/390 of query aligns to 11:411/2214 of P07259

query
sites
P07259

T

T

P

P

S

M

D

E

N

S

A

T

V

G

H

D

T

R

G

L

V

V

-

T

L

L

V

E

L

L

A

K

D

D

G

G

T

T

V

V

F

L

R

Q

G

G

R

Y

G

S

I

F

G

G

A

A

T

E

G

K

D

S

S

V

V

A

G

G

E

E

V

L

C

V

F

F

N

Q

T

T

S

G

M

M

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

V

T

T

D

D

P

P

S

S

Y

Y

A

E

G

G

Q

Q

I

I

I

L

T

V

F

I

T

T

F

Y

P

P

H

L

I

V

G

G

N

N

T

Y

G

G

A

V

N

P

-

D

-

M

-

H

-

L

-

R

-

D

-

E

-

L

A

V

E

E

D

E

I

L

E

P

T

R

I

Y

T

F

P

E

A

S

A

N

R

R

-

I

-

H

-

I

-

A

G

G

L

L

I

V

L

I

R

S

A

H

D

Y

I

T

T

D

D

E

P

Y

S

S

N

H

W

Y

R

L

A

A

T

K

R

S

H

S

L

L

D

G

D

K

W

W

L

L

K

Q

S

N

Y

E

G

G

L

I

V

P

G

A

L

V

A

Y

G

G

V

V

D

D

T

T

R

R

R

S

L

L

T

T

R

K

R

H

I

L

R

R

D

D

L

A

G

G

A

S

P

M

N

L

G

G

V

R

V

L

A

S

H

L

A

E

P

K

D

S

G

G

R

S

F

-

D

-

I

-

D

D

A

R

L

T

V

I

A

S

K

R

A

S

K

S

G

S

W

W

-

R

-

S

-

A

-

F

-

D

-

V

-

P

-

E

-

W

-

V

-

D

P

P

G

N

L

V

E

Q

G

-

M

-

D

N

L

L

A

V

K

S

D

K

V

V

S

S

C

I

R

N

Q

E

T

P

Y

K

D

L

W

Y

T

V

E

P

A

P

A

A

W

D

T

N

I

K

G

H

G

I

G

E

Y

L

A

Q

T

T

-

G

Q

P

D

D

K

G

P

K

R

V

Y

L

H

R

V

I

V

L

A

A

I

I

D

D

Y

V

G

G

A

M

K

K

R

Y

N

N

I

Q

L

I

R

R

C

C

L

F

A

I

A

K

A

R

G

G

C

V

K

E

V

L

T

K

V

V

P

P

S

W

T

N

A

Y

S

-

V

-

E

-

D

D

V

F

M

T

R

K

H

E

Q

D

P

Y

D

D

G

G

V

L

F

F

L

I

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

V

T

L

G

D

E

D

Y

L

A

S

V

Q

P

R

T

L

I

S

K

N

G

V

L

L

L

E

D

A

T

K

G

K

V

T

P

P

M

V

F

F

G

G

I

I

C

C

L

L

G

G

H

H

Q

Q

M

L

L

I

S

A

L

R

A

A

L

A

G

G

A

A

K

S

T

T

T

L

K

K

M

L

Q

K

Q

F

G

G

H

N

R

R

G

G

A

H

N

N

H

I

P

P

V

C

K

T

D

S

L

T

V

I

S

S

G

G

R

R

V

C

E

Y

I

I

T

T

S

S

Q

Q

N

N

H

H

G

G

F

F

V

A

V

V

V

D

P

V

E

D

T

T

L

L

P

T

A

S

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

A

F

N

D

D

G

D

T

S

N

N

E

E

G

G

I

I

R

Y

L

H

K

S

G

E

K

L

P

P

V

Y

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

S

S

T

P

P

G

G

P

P

Q

R

D

D

S

T

H

E

Y

F

L

L

F

F

D

D

R

V

F

F

V

I

Q

Q

L

A

I

V

A

K

E

E

K

F

K

K

Sites not aligning to the query:

1857 modified: Phosphoserine; by PKA

Q09794 Multifunctional protein ura1; Pyrimidine-specific carbamoyl phosphate synthase-aspartate carbamoyl transferase; CPSase-ATCase; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
36% identity, 94% coverage: 17:384/390 of query aligns to 61:443/2244 of Q09794

query
sites
Q09794

L

L

V

E

L

L

A

E

D

D

G

K

T

S

V

V

F

L

R

Q

G

G

R

Y

G

S

I

F

G

G

A

A

T

D

G

H

D

S

S

V

V

S

G

G

E

E

V

L

C

V

F

F

N

Q

T

T

S

G

M

M

T

V

G

G

Y

Y

Q

P

E

E

I

S

L

L

T

T

D

D

P

P

S

S

Y

Y

A

R

G

G

Q

Q

I

I

I

L

T

V

F

F

T

T

F

F

P

P

H

T

I

V

G

G

N

N

T

Y

G

G

A

V

N

P

A

D

E

R

D

R

I

I

-

L

-

D

-

E

-

I

-

S

-

G

-

L

-

P

-

K

-

Y

-

F

E

E

T

S

I

N

T

Q

P

I

A

H

A

V

R

A

G

A

L

I

I

I

L

I

R

S

A

S

D

Y

I

S

T

Q

D

N

P

Y

S

S

N

H

W

F

R

L

A

A

T

H

R

S

H

S

L

L

D

G

D

E

W

W

L

L

K

K

S

E

Y

Q

G

G

L

I

V

P

G

G

L

I

A

F

G

G

V

I

D

D

T

T

R

R

R

A

L

L

T

T

R

K

I

I

R

R

D

D

L

Q

G

G

A

S

P

M

N

L

G

G

-

R

V

L

A

I

H

Q

A

K

P

D

D

E

G

S

R

P

F

I

D

N

I

P

D

S

A

S

L

I

V

T

A

G

K

L

A

G

K

K

G

D

W

W

-

S

-

T

-

A

-

F

-

E

-

D

-

I

-

P

-

W

-

K

-

N

P

P

G

N

L

T

E

E

G

-

M

-

D

N

L

L

A

T

K

S

D

Q

V

V

S

S

C

I

R

K

Q

E

T

P

Y

K

D

L

W

Y

T

E

E

P

A

H

A

P

W

T

T

T

I

-

G

-

G

A

Y

I

A

K

T

K

Q

A

D

D

K

G

P

K

R

I

Y

I

H

R

V

I

V

L

A

V

I

I

D

D

Y

V

G

G

A

M

K

K

R

Y

N

N

I

Q

L

I

R

R

C

C

L

F

A

L

A

N

A

R

G

G

C

V

K

E

V

L

T

L

V

V

P

P

S

W

T

D

A

Y

S

-

V

-

E

-

D

D

V

F

M

T

R

K

H

E

Q

T

P

Y

D

D

G

G

V

L

F

F

L

I

S

S

N

N

G

G

P

P

G

G

D

D

P

P

A

S

A

L

T

M

G

-

E

D

Y

L

A

V

V

V

P

D

T

R

I

V

K

K

G

R

L

V

L

L

D

E

T

S

G

K

-

T

V

V

P

P

M

V

F

F

G

G

I

I

C

C

L

F

G

G

H

H

Q

Q

M

I

L

M

S

A

L

R

A

A

L

A

G

G

A

A

K

S

T

T

K

K

M

M

Q

K

Q

F

G

G

H

N

R

R

G

G

A

H

N

N

H

I

P

P

V

C

K

T

D

C

L

M

V

I

S

S

G

G

R

R

V

C

E

Y

I

I

T

T

S

S

Q

Q

N

N

H

H

G

G

F

Y

V

A

V

V

V

D

P

A

E

S

T

S

L

L

P

S

A

N

D

G

A

W

E

K

V

E

T

L

H

F

V

V

S

N

L

A

F

N

D

D

G

G

T

S

N

N

E

E

G

G

I

I

R

Y

L

N

K

T

G

E

K

Y

P

P

V

F

F

F

S

S

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

S

S

T

P

P

G

G

P

P

Q

R

D

D

S

T

H

E

Y

F

L

L

F

F

D

D

R

V

F

F

V

I

Q

D

L

V

I

V

Sites not aligning to the query:

1119 modified: Phosphoserine
1881 modified: Phosphoserine
1885 modified: Phosphoserine

P05990 Multifunctional protein r; Protein rudimentary; EC 6.3.5.5; EC 3.5.1.2; EC 6.3.4.16; EC 2.1.3.2; EC 3.5.2.3 from Drosophila melanogaster (Fruit fly) (see 2 papers)
38% identity, 95% coverage: 17:387/390 of query aligns to 8:377/2224 of P05990

query
sites
P05990

L

L

V

A

L

L

A

E

D

D

G

G

T

T

V

V

F

L

R

P

G

G

R

Y

G

S

I

F

G

G

-

Y

-

V

-

P

A

S

T

E

G

N

D

E

S

S

V

K

-

V

-

G

-

F

-

G

G

G

E

E

V

V

C

V

F

F

N

Q

T

T

S

G

M

M

T

V

G

G

Y

Y

Q

T

E

E

I

A

L

L

T

T

D

D

P

R

S

S

Y

Y

A

S

G

G

Q

Q

I

I

I

L

T

V

F

L

T

T

F

Y

P

P

H

L

I

I

G

G

N

N

T

Y

G

G

A

V

N

P

A

A

E

P

D

D

I

E

E

D

T

E

I

H

-

G

-

L

-

P

-

L

-

H

-

F

-

E

-

W

-

M

-

K

-

G

T

V

P

V

A

Q

A

A

R

T

G

A

L

L

I

V

L

V

R

G

A

E

D

V

I

A

T

E

D

E

P

A

S

F

N

H

W

W

R

R

A

K

T

W

R

K

H

T

L

L

D

P

D

D

W

W

L

L

K

K

S

Q

Y

H

G

K

L

V

V

P

G

G

L

I

A

Q

G

D

V

I

D

D

T

T

R

R

R

A

L

L

T

T

R

K

I

L

R

R

D

E

L

Q

G

G

A

S

P

M

N

L

G

G

V

K

V

I

A

V

H

Y

A

E

P

-

D

-

G

-

R

K

F

P

D

P

I

V

D

E

A

G

L

L

V

P

A

K

K

S

A

S

K

F

G

V

W

D

P

P

G

N

L

V

E

R

G

N

M

L

D

A

L

K

A

E

K

C

D

S

V

V

S

K

C

E

R

R

Q

Q

T

V

Y

Y

D

-

W

-

T

-

E

-

A

-

W

-

T

-

I

-

G

-

G

G

Y

N

A

P

T

N

Q

G

D

K

K

G

P

P

R

R

Y

-

H

-

V

I

V

A

A

I

I

L

D

D

Y

C

G

G

A

L

K

K

R

L

N

N

I

Q

L

L

R

R

C

C

L

L

A

L

A

Q

A

R

G

G

C

A

K

S

V

V

T

T

V

L

P

P

S

W

T

S

A

A

S

R

V

L

E

E

D

D

V

-

M

-

R

-

H

E

Q

Q

P

F

D

D

G

A

V

L

F

F

L

L

S

S

N

N

G

G

P

P

G

G

D

N

P

P

A

E

A

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K

Sites not aligning to the query:

1167 E→K: Severely diminishes UTP inhibition of CPSase; in Su(b).
1883 modified: Phosphoserine
1885 modified: Phosphoserine
1892 modified: Phosphoserine
1894 modified: Phosphoserine

P07258 Carbamoyl phosphate synthase arginine-specific small chain; CPS; CPSase; CPSase-arg; Arginine-specific carbamoyl phosphate synthetase, glutamine chain; Carbamoyl phosphate synthase A; CPS-A; Glutamine-dependent carbamoyl phosphate synthetase; EC 6.3.5.5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
37% identity, 97% coverage: 10:386/390 of query aligns to 3:372/411 of P07258

query
sites
P07258

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C264 (= C279) mutation to D: Abolishes glutamine-dependent CPSase activity.
H307 (= H322) mutation to D: Abolishes glutamine-dependent CPSase activity.
H349 (= H363) mutation to D: Abolishes glutamine-dependent CPSase activity.

P31327 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Homo sapiens (Human) (see 22 papers)
36% identity, 97% coverage: 13:389/390 of query aligns to 44:403/1500 of P31327

query
sites
P31327

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K55 (≠ V24) modified: N6-glutaryllysine; alternate
S123 (≠ T82) to F: in CPS1D; modestly decreases enzyme activity; to Y: in CPS1D; uncertain significance
K171 (≠ R130) modified: N6-glutaryllysine; alternate
R174 (= R133) to W: in CPS1D; uncertain significance; dbSNP:rs1553509661
K176 (≠ L135) modified: N6-glutaryllysine
K207 (≠ R177) modified: N6-glutaryllysine; alternate
K210 (≠ Y180) modified: N6-glutaryllysine; alternate
K214 (≠ G191) modified: N6-glutaryllysine; alternate
K219 (≠ Q196) modified: N6-glutaryllysine; alternate
K228 (= K211) modified: N6-glutaryllysine; alternate
K237 (≠ A220) modified: N6-glutaryllysine
K280 (≠ G267) modified: N6-glutaryllysine; alternate
A304 (≠ L289) to V: in CPS1D; associated with T-986; dbSNP:rs775920437
K307 (= K292) modified: N6-glutaryllysine; alternate
K310 (= K295) modified: N6-glutaryllysine; alternate
H337 (= H322) to R: in CPS1D; modestly decreases enzyme activity; dbSNP:rs28940283
T344 (= T330) to A: no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs1047883
N355 (≠ S341) to D: in CPS1D; around 80% decrease in enzyme activity; significant reduction in thermal stability; approximately 4-fold decrease in the apparent Vmax for ATP, bicarbonate and ammonia; dbSNP:rs1472190012
Y389 (= Y375) to C: in CPS1D; around 40% decrease in enzyme activity; significant loss of thermal stability
L390 (= L376) to R: in CPS1D; significant loss of protein stability
G401 (≠ K387) to R: in CPS1D; uncertain significance; associated with N-937 in a patient; dbSNP:rs760895692
K402 (= K388) modified: N6-glutaryllysine; alternate

Sites not aligning to the query:

412 modified: N6-glutaryllysine; alternate
438 A → P: in CPS1D; almost complete loss of enzyme activity; dbSNP:rs772497399
453 modified: N6-glutaryllysine; alternate
458 modified: N6-glutaryllysine; alternate
527 modified: N6-glutaryllysine; alternate
530 G → V: found in a patient with VACTERL syndrome and postsurgical PHN; uncertain significance; dbSNP:rs1250316045
532 modified: N6-glutaryllysine; alternate
544 T → M: in CPS1D; almost complete loss of enzyme activity; approximately 60-fold increase in the apparent Km for bicarbonate and approximately 4-fold respective decrease and increase in the apparent Vmax and Km for ammonia; dbSNP:rs121912592
553 modified: N6-glutaryllysine; alternate
678 Q → P: in CPS1D; results in a poor enzyme expression and solubility; hampers correct enzyme folding
728 modified: N6-glutaryllysine
757 modified: N6-glutaryllysine; alternate
772 modified: N6-glutaryllysine; alternate
774 P → L: in CPS1D; the enzyme is inactive
793 modified: N6-glutaryllysine; alternate
811 modified: N6-glutaryllysine; alternate
841 modified: N6-glutaryllysine; alternate
843 L → S: in CPS1D; associated in cis with E-875; causes 70% decrease of enzyme activity; significant decrease in protein yield
850 R → C: in CPS1D; moderate decrease in protein yield and partial loss of enzyme activity; dbSNP:rs1015051007; R → H: in CPS1D; partial loss of enzyme activity; dbSNP:rs767694281
856 modified: N6-glutaryllysine; alternate
869 modified: N6-glutaryllysine
871 T → P: in CPS1D; significant decrease in protein yield and enzyme activity
875 modified: N6-glutaryllysine; alternate; K → E: associated in cis with S-843 in a patient with carbamoyl-phosphate synthase deficiency; does not affect enzyme activity; significant decrease in protein yield and thermal stability; dbSNP:rs147062907
889 modified: N6-glutaryllysine; alternate
892 modified: N6-glutaryllysine; alternate
905 modified: N6-glutaryllysine
908 modified: N6-glutaryllysine; alternate
911 G → E: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs1388955593; G → V: in CPS1D; significant decrease in protein yield and enzyme activity
913 S → L: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs754706559
914 D → G: in CPS1D; significant decrease in protein yield and enzyme activity; D → H: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs765484849
915 modified: N6-glutaryllysine; alternate
918 S → P: in CPS1D; significant decrease in protein yield and enzyme activity
919 modified: N6-glutaryllysine; alternate
932 R → T: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity
937 I → N: in CPS1D; associated with R-401; significant decrease in protein yield and enzyme activity; dbSNP:rs760714614
949 A → T: in CPS1D; partial loss of enzyme activity and significant decrease in thermal stability; dbSNP:rs537170841
958 L → P: in CPS1D; significant decrease in protein yield and enzyme activity
959 Y → C: in CPS1D; significant decrease in protein yield and thermal stability; partial loss of enzyme activity; dbSNP:rs1191587211
962 Y → C: in CPS1D; significant decrease in protein yield and partial loss of enzyme activity; dbSNP:rs955666400
964 G → D: in CPS1D; significant decrease in protein yield and enzyme activity; dbSNP:rs534815243
986 I → T: in CPS1D; associated with V-304; dbSNP:rs1553516442
987 G → C: in CPS1D; may affect splicing; dbSNP:rs1553516443
1074 modified: N6-glutaryllysine; alternate
1150 modified: N6-glutaryllysine
1168 modified: N6-glutaryllysine; alternate
1183 modified: N6-glutaryllysine; alternate
1215 I → V: in CPS1D; uncertain significance; dbSNP:rs141373204
1224 modified: N6-glutaryllysine
1254 I → F: in CPS1D; uncertain significance
1266 F → S: in dbSNP:rs1047886
1283 M → L: in dbSNP:rs1047887
1356 modified: N6-glutaryllysine; alternate
1360 modified: N6-glutaryllysine; alternate
1363:1366 natural variant: Missing (in CPS1D; uncertain significance)
1376 G → S: no functional consequences; no negative effect on protein stability, enzyme activity and thermal stability; dbSNP:rs140578009
1378 A → T: in CPS1D; significant reduction in thermal stability; dbSNP:rs1245373037
1381 L → S: in CPS1D; significant loss of protein stability
1406 T → N: probable risk factor for PHN; dbSNP:rs1047891
1411 P → L: in CPS1D; modestly decreases enzyme activity; dbSNP:rs1202306773
1443 T → A: in CPS1D; almost complete loss of enzyme activity; approximately 10-fold decrease in the apparent Vmax for bicarbonate, ammonia and ATP; decreased affinity for NAG
1453 R → Q: in CPS1D; the enzyme is inactive; R → W: in CPS1D; the enzyme is inactive; dbSNP:rs933813349
1479 modified: N6-glutaryllysine; alternate
1486 modified: N6-glutaryllysine; alternate
1491 Y → H: in CPS1D; triggers a large decrease in the apparent affinity for N-acetyl-L-glutamate (NAG); dbSNP:rs1553519513

6w2jA Cps1 bound to allosteric inhibitor h3b-374 (see paper)
36% identity, 97% coverage: 13:389/390 of query aligns to 2:361/1422 of 6w2jA

query
sites
6w2jA

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D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

N

P

-

D

-

T

-

A

-

L

-

D

-

E

-

L

-

G

-

L

A

S

E

K

D

Y

I

L

E

E

T

S

I

N

T

G

P

I

A

K

A

V

R

S

G

G

L

L

I

L

L

V

R

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

P

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

T

R

K

Q

D

T

V

Y

K

D

V

W

Y

T

-

E

-

A

-

W

-

T

-

I

-

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

K

H

M

Q

E

P

Y

D

D

G

G

V

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

E

Y

P

A

L

V

I

P

Q

T

N

I

V

K

R

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G

K

V

E

P

P

M

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

T

Y

K

K

M

M

Q

S

Q

M

G

A

H
x
N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

K

R

Q

V

A

E

F

I

I

T

T

S

A

Q
|
Q

N

N

H
|
H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E
|
E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

A

A

binding zinc ion: N273 (≠ H300), Q293 (= Q320), H295 (= H322), E320 (= E348)

Sites not aligning to the query:

active site: 651, 665, 667, 673, 869, 995, 1113, 1249
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 605, 607, 615, 725, 726, 727, 730, 732, 756, 760, 763, 764, 795, 797, 798

6w2jB Cps1 bound to allosteric inhibitor h3b-374 (see paper)
36% identity, 97% coverage: 13:389/390 of query aligns to 2:361/1364 of 6w2jB

query
sites
6w2jB

H

Q

T

T

G

A

V

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

N

P

-

D

-

T

-

A

-

L

-

D

-

E

-

L

-

G

-

L

A

S

E

K

D

Y

I

L

E

E

T

S

I

N

T

G

P

I

A

K

A

V

R

S

G

G

L

L

I

L

L

V

R

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

P

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

T

R

K

Q

D

T

V

Y

K

D

V

W

Y

T

-

E

-

A

-

W

-

T

-

I

-

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

K

H

M

Q

E

P

Y

D

D

G

G

V

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

E

Y

P

A

L

V

I

P

Q

T

N

I

V

K

R

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G

K

V

E

P

P

M

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

T

Y

K

K

M

M

Q

S

Q

M

G

A

H
x
N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

K

R

Q

V

A

E

F

I

I

T

T

S

A

Q
|
Q

N

N

H
|
H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E
|
E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

A

A

binding zinc ion: N273 (≠ H300), Q293 (= Q320), H295 (= H322), E320 (= E348)

Sites not aligning to the query:

active site: 593, 607, 609, 615, 811, 937, 1055, 1191
binding (2-fluoranyl-4-methoxy-phenyl)-[(3~{R},5~{R})-4-(2-fluoranyl-4-methoxy-phenyl)carbonyl-3,5-dimethyl-piperazin-1-yl]methanone: 547, 549, 557, 668, 669, 672, 673, 698, 702, 705, 706, 737, 739, 740

6uelA Cps1 bound to allosteric inhibitor h3b-193 (see paper)
36% identity, 97% coverage: 13:389/390 of query aligns to 2:361/1333 of 6uelA

query
sites
6uelA

H

Q

T

T

G

A

V

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

N

P

-

D

-

T

-

A

-

L

-

D

-

E

-

L

-

G

-

L

A

S

E

K

D

Y

I

L

E

E

T

S

I

N

T

G

P

I

A

K

A

V

R

S

G

G

L

L

I

L

L

V

R

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

P

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

T

R

K

Q

D

T

V

Y

K

D

V

W

Y

T

-

E

-

A

-

W

-

T

-

I

-

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

K

H

M

Q

E

P

Y

D

D

G

G

V

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

E

Y

P

A

L

V

I

P

Q

T

N

I

V

K

R

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G

K

V

E

P

P

M

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

L

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

T

Y

K

K

M

M

Q

S

Q

M

G

A

H
x
N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

K

R

Q

V

A

E

F

I

I

T

T

S

A

Q
|
Q

N

N

H
|
H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E
|
E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

A

A

binding zinc ion: N273 (≠ H300), Q293 (= Q320), H295 (= H322), E320 (= E348)

Sites not aligning to the query:

active site: 587, 601, 603, 609, 803, 929, 1032, 1168
binding N~1~-[(4-fluorophenyl)methyl]-N~1~-methyl-N~4~-(4-methyl-1,3-thiazol-2-yl)piperidine-1,4-dicarboxamide: 544, 551, 662, 663, 665, 690, 698, 701, 729, 731, 732

5douD Crystal structure of human carbamoyl phosphate synthetase i (cps1), ligand-bound form (see paper)
36% identity, 97% coverage: 13:389/390 of query aligns to 2:361/1430 of 5douD

query
sites
5douD

H

Q

T

T

G

A

V

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

I

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

N

P

-

D

-

T

-

A

-

L

-

D

-

E

-

L

-

G

-

L

A

S

E

K

D

Y

I

L

E

E

T

S

I

N

T

G

P

I

A

K

A

V

R

S

G

G

L

L

I

L

L

V

R

L

A

D

D

Y

I

S

T

K

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

P

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

T

R

K

Q

D

T

V

Y

K

D

V

W

Y

T

-

E

-

A

-

W

-

T

-

I

-

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

K

H

M

Q

E

P

Y

D

D

G

G

V

I

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

E

Y

P

A

L

V

I

P

Q

T

N

I

V

K

R

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G

K

V

E

P

P

M

L

F

F

G

G

I

I

C
x
S

L

T

G

G

H

N

Q

L

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

T

T

Y

K

K

M

M

Q

S

Q

M

G

A

H

N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

K

R

Q

V

A

E

F

I

I

T

T

S

A

Q

Q

N

N

H

H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E

E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H
|
H

P

P

E
|
E

A

V

S

T

P

P

G

G

P

P

Q

I

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

A

A

active site: S252 (≠ C279), H335 (= H363), E337 (= E365)

Sites not aligning to the query:

active site: 505, 545, 576, 589, 617, 656, 658, 672, 674, 676, 680, 880, 1006, 1087, 1116, 1124, 1184, 1196, 1198, 1203, 1260
binding adenosine-5'-diphosphate: 505, 543, 545, 550, 551, 552, 581, 583, 584, 585, 589, 614, 615, 616, 617, 658, 671, 672, 1085, 1110, 1111, 1116, 1140, 1142, 1143, 1144, 1184, 1186, 1195, 1196
binding magnesium ion: 658, 672, 672, 674
binding n-acetyl-l-glutamate: 1307, 1308, 1332, 1334, 1335, 1351, 1379, 1384, 1385, 1386, 1390
binding phosphate ion: 550, 551, 617, 672, 674, 676, 679

Q8C196 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Mus musculus (Mouse) (see 2 papers)
37% identity, 96% coverage: 14:388/390 of query aligns to 45:402/1500 of Q8C196

query
sites
Q8C196

T

T

G

A

V

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

P

E

E

I

A

L

L

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

-

P

-

D

-

T

N

T

A

A

E

R

D

D

-

E

-

L

-

G

-

L

-

N

-

K

-

Y

I

M

E

E

T

S

I

D

T

G

P

I

A

K

A

V

R

A

G

G

L

L

I

L

L

V

R

L

A

N

D

Y

I

S

T

N

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

S

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

-

R

-

Q

-

T

-

Y

-

D

-

W

-

T

T

E

K

A

D

A

V

W

K

T

V

I

F

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

Q

H

M

Q

E

P

Y

D

D

G

G

V

L

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

Q

Y

P

A

L

V

I

P

Q

T

N

I

V

K

K

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G
x
K

V

E

P

P

M

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

I

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

S

T

Y

K

K

M

M

Q

S

Q

M

G

A

H

N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

R

R

Q

V

A

E

F

I

I

T

T

S

A

Q

Q

N

N

H

H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E

E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

V

S

S

P

P

G

G

P

P

Q

T

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

K287 (≠ G272) modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

Sites not aligning to the query:

44 modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate
1291 modified: N6-acetyllysine; alternate; modified: N6-succinyllysine; alternate

P07756 Carbamoyl-phosphate synthase [ammonia], mitochondrial; Carbamoyl-phosphate synthetase I; CPSase I; EC 6.3.4.16 from Rattus norvegicus (Rat) (see 2 papers)
37% identity, 96% coverage: 13:388/390 of query aligns to 44:402/1500 of P07756

query
sites
P07756

H

Q

T

T

G

A

V

H

L

I

V

V

L

L

A

E

D

D

G

G

T

T

V

K

F

M

R

K

G

G

R

Y

G

S

I

F

G

G

A

H

T

P

G

S

D

S

S

V

V

A

G

G

E

E

V

V

C

V

F

F

N

N

T

T

S

G

M

L

T

G

G

G

Y

Y

Q

S

E

E

I

A

L

L

T

T

D

D

P

P

S

A

Y

Y

A

K

G

G

Q

Q

I

I

I

L

T

T

F

M

T

A

F

N

P

P

H

I

I

I

G

G

N

N

T

G

G

G

A

A

-

P

-

D

-

T

N

T

A

A

E

R

D

D

-

E

-

L

-

G

-

L

-

N

-

K

-

Y

I

M

E

E

T

S

I

D

T

G

P

I

A

K

A

V

R

A

G

G

L

L

I

L

L

V

R

L

A

N

D

Y

I

S

T

H

D

D

P

Y

S

N

N

H

W

W

R

L

A

A

T

T

R

K

H

S

L

L

D

G

D

Q

W

W

L

L

K

Q

S

E

Y

E

G

K

L

V

V

P

G

A

L

I

A

Y

G

G

V

V

D

D

T

T

R

R

R

M

L

L

T

T

R

K

R

I

I

I

R

R

D

D

L

K

G

G

A

T

P

M

N

L

G

G

V

K

V

I

A

E

H

F

A

E

P

G

D

Q

G

S

R

V

F

D

D

F

I

V

D

D

A

P

L

-

V

-

A

-

K

-

A

-

K

-

G

-

W

-

P

-

G

-

L

-

E

N

G

K

M

Q

D

N

L

L

A

I

K

A

D

E

V

V

S

S

C

-

R

-

Q

-

T

-

Y

-

D

-

W

-

T

T

E

K

A

D

A

V

W

K

T

V

I

F

G

G

G

K

G

G

Y

N

A

P

T

T

Q

K

D

-

K

-

P

-

R

-

Y

-

H

-

V

V

A

A

I

V

D

D

Y

C

G

G

A

I

K

K

R

N

N

N

I

V

L

I

R

R

C

L

L

L

A

V

A

K

A

R

G

G

C

A

K

E

V

V

T

H

V

L

V

V

P

P

S

W

T

N

A

-

S

-

V

-

E

H

D

D

V

F

M

T

R

Q

H

M

Q

D

P

Y

D

D

G

G

V

L

F

L

L

I

S

A

N

G

G

G

P

P

G

G

D

N

P

P

A

A

A

-

T

L

G

A

E

Q

Y

P

A

L

V

I

P

Q

T

N

I

V

K

K

G

K

L

I

L

L

-

E

-

S

D

D

T

R

G

K

V

E

P

P

M

L

F

F

G

G

I

I

C

S

L

T

G

G

H

N

Q

I

M

I

L

T

S

G

L

L

A

A

L

A

G

G

A

A

K

K

T

S

T

Y

K

K

M

M

Q

S

Q

M

G

A

H

N

R

R

G

G

A

Q

N

N

H

Q

P

P

V

V

K

L

D

N

L

I

V

T

S

N

G

R

R

Q

V

A

E

F

I

I

T

T

S

A

Q

Q

N

N

H

H

G

G

F

Y

V

A

V

L

V

-

P

D

E

N

T

T

L

L

P

P

A

A

D

G

A

W

E

K

V

P

T

L

H

F

V

V

S

N

L

V

F

N

D

D

G

Q

T

T

N

N

E

E

G

G

I

I

R

M

L

H

K

E

G

S

K

K

P

P

V

F

F

F

S

A

V

V

Q

Q

Y

F

H

H

P

P

E

E

A

V

S

S

P

P

G

G

P

P

Q

T

D

D

S

T

H

E

Y

Y

L

L

F

F

D

D

R

S

F

F

V

F

Q

S

L

L

I

I

A

K

E

K

K

G

K

K

Sites not aligning to the query:

537 modified: carbohydrate, O-linked (GlcNAc) serine; alternate
1331 modified: carbohydrate, O-linked (GlcNAc) serine
1332 modified: carbohydrate, O-linked (GlcNAc) threonine
1391 T→V: 400-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
1394 T→A: 900-fold increase in the activation constant of NAG. 3-fold decrease in the reaction rate at saturation of NAG.
1410 W→K: 60-fold increase in the activation constant of NAG.
1437 N→D: 70-fold increase in the activation constant of NAG.
1440 N→D: 110-fold increase in the activation constant of NAG. Modifies the specificity for the activator: Binds Phe-NAG considerably better than NAG.

2ywcA Crystal structure of gmp synthetase from thermus thermophilus in complex with xmp
27% identity, 48% coverage: 203:388/390 of query aligns to 2:188/475 of 2ywcA

query
sites
2ywcA

V

V

V

L

A

V

I

L

D

D

Y

F

G

G

A

S

K

Q

-

Y

-

T

R

R

N

L

I

I

L

A

R

R

C

R

L

L

A

R

A

E

A

L

G

R

C

A

K

F

V

S

T

L

V

I

V

L

P

P

S

G

T

D

A

A

S

P

V

L

E

E

D

E

V

V

M

L

R

K

H

H

Q

R

P

P

D

Q

G

A

V

L

F

I

L

L

S

S

N

G

G
|
G

P

P

G
x
R

-

S

-

V

-

F

D

D

P

P

A

D

A

A

T

P

G

R

E

-

Y

-

A

-

V

-

P

P

T

D

I

P

K

R

G

-

L

L

L

F

D

S

T

S

G

G

V

L

P

P

M

L

F

L

G

G

I

I

C
|
C

L
x
Y

G

G

H

M

Q

Q

M

L

L

L

S

A

L

Q

A

E

L

L

G

G

A

G

K

R

T

V

T

E

K

R

M

A

Q

G

Q

R

G

A

H

E

-

Y

-

G

-

K

-

A

-

L

-

T

-

R

-

H

-

E

-

G

-

P

-

L

-

F

R

R

G

G

A

L

N

E

H

G

P

E

V

V

K

Q

D

V

L

W

V

M

S

S

G

H

R

Q

V

D

E

A

I

V

T

T

S

A

Q

P

N

P

H

P

G

G

F

W

V

R

V

V

P

A

E

E

T

T

L

-

P

-

A

E

D

E

A

N

E

P

V

V

T

A

H

A

V

I

S

A

L

S

F

P

D

D

G

G

T

R

N

A

E

Y

G

G

I

-

R

-

L

-

K

-

G

-

K

-

P

-

V

-

F

-

S

-

V

V

Q

Q

Y

F

H
|
H

P

P

E
|
E

A

V

S

A

P

H

G

T

P

P

Q

K

D

G

S

M

H

Q

Y

I

L

L

F

-

D

E

R

N

F

F

V

L

Q

E

L

L

I

A

A

G

E

V

K

K

K

R

active site: G51 (= G250), R53 (≠ G252), C78 (= C279), Y79 (≠ L280), H164 (= H363), E166 (= E365)

Sites not aligning to the query:

active site: 221, 343
binding xanthosine-5'-monophosphate: 288, 366, 367, 368, 408, 467, 471, 472, 473

Query Sequence

>WP_012973676.1 NCBI__GCF_000010725.1:WP_012973676.1
MTLATPPSDNAVHTGVLVLADGTVFRGRGIGATGDSVGEVCFNTSMTGYQEILTDPSYAG
QIITFTFPHIGNTGANAEDIETITPAARGLILRADITDPSNWRATRHLDDWLKSYGLVGL
AGVDTRRLTRRIRDLGAPNGVVAHAPDGRFDIDALVAKAKGWPGLEGMDLAKDVSCRQTY
DWTEAAWTIGGGYATQDKPRYHVVAIDYGAKRNILRCLAAAGCKVTVVPSTASVEDVMRH
QPDGVFLSNGPGDPAATGEYAVPTIKGLLDTGVPMFGICLGHQMLSLALGAKTTKMQQGH
RGANHPVKDLVSGRVEITSQNHGFVVVPETLPADAEVTHVSLFDGTNEGIRLKGKPVFSV
QYHPEASPGPQDSHYLFDRFVQLIAEKKAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory