SitesBLAST
Comparing WP_012973715.1 NCBI__GCF_000010725.1:WP_012973715.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
48% identity, 94% coverage: 27:434/434 of query aligns to 22:433/435 of Q5F8J4
- L45 (≠ R50) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ RS 50:51) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
44% identity, 98% coverage: 6:432/434 of query aligns to 2:429/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G15), L12 (= L16), G13 (= G17), N14 (≠ T18), V15 (= V19), V45 (≠ A49), R46 (= R50), R47 (≠ S51), R52 (= R56), I63 (≠ V70), L78 (= L84), M79 (≠ I85), P84 (= P91), A102 (= A109), K104 (= K111), G306 (= G308), T310 (= T312)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
37% identity, 74% coverage: 5:324/434 of query aligns to 1:307/402 of 4pg7A
Sites not aligning to the query:
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 66% coverage: 9:294/434 of query aligns to 4:276/331 of 6a0sA
- active site: D191 (= D207), K195 (= K211)
- binding l-homoserine: K99 (= K111), N150 (= N165), G151 (= G166), T152 (= T167), Y178 (= Y194), E180 (= E196), D186 (= D202), K195 (= K211)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L16), G12 (= G17), T13 (= T18), V14 (= V19), L42 (≠ S48), V43 (≠ A49), R44 (= R50), D45 (≠ S51), K48 (= K54), R50 (= R56), A73 (≠ L84), M74 (≠ I85), A97 (= A109), K99 (= K111), G177 (= G193), E180 (= E196)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 66% coverage: 9:294/434 of query aligns to 4:276/331 of 2ejwA
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 66% coverage: 9:294/434 of query aligns to 4:276/332 of 6a0tB
- active site: D191 (= D207), K195 (= K211)
- binding l-homoserine: N150 (= N165), G151 (= G166), T152 (= T167), Y178 (= Y194), E180 (= E196), D186 (= D202), K195 (= K211)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G15), G11 (≠ L16), G12 (= G17), T13 (= T18), V14 (= V19), L42 (≠ S48), V43 (≠ A49), R44 (= R50), D45 (≠ S51), K48 (= K54), R50 (= R56), A73 (≠ L84), M74 (≠ I85), G75 (= G86), A97 (= A109), N98 (= N110), G177 (= G193), E180 (= E196)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
31% identity, 73% coverage: 9:325/434 of query aligns to 1:297/300 of 7f4cA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ A14), G7 (= G15), Y8 (≠ L16), G9 (= G17), N10 (≠ T18), V11 (= V19), T37 (≠ S51), R38 (≠ K52), R39 (= R56), V72 (≠ L84), S73 (≠ I85), S74 (≠ G86), T100 (≠ A109), K102 (= K111), G127 (≠ A136), S131 (≠ G140), E185 (= E196), G280 (= G310), A284 (≠ S314)
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
32% identity, 73% coverage: 9:325/434 of query aligns to 1:301/304 of F9VNG5
- Y8 (≠ L16) binding NADP(+)
- N10 (≠ T18) binding NADP(+)
- V11 (= V19) binding NADP(+)
- R38 (≠ K52) binding NADP(+)
- R39 (= R56) binding NADP(+)
- S73 (≠ I85) binding NADP(+)
- T100 (≠ A109) binding NADP(+)
- K102 (= K111) binding NADP(+)
- G182 (= G193) binding NADP(+)
- E185 (= E196) binding NADP(+)
- G284 (= G310) binding NADP(+)
Sites not aligning to the query:
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
32% identity, 73% coverage: 9:325/434 of query aligns to 1:299/302 of 5x9dA
- active site: D196 (= D207), K200 (= K211)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ A14), G7 (= G15), Y8 (≠ L16), G9 (= G17), N10 (≠ T18), V11 (= V19), T37 (≠ S51), R38 (≠ K52), V72 (≠ L84), S73 (≠ I85), S74 (≠ G86), P82 (= P91), T100 (≠ A109), N101 (= N110), K102 (= K111), G127 (≠ A136), S131 (≠ G140), N155 (= N165), G156 (= G166), T157 (= T167), Y183 (= Y194), A184 (= A195), E185 (= E196), D191 (= D202), D196 (= D207), K200 (= K211), A281 (= A309), G282 (= G310), A286 (≠ S314)
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
33% identity, 53% coverage: 9:238/434 of query aligns to 3:240/319 of 3ingA
- active site: D209 (= D207), K213 (= K211)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G15), T10 (≠ L16), G11 (= G17), N12 (≠ T18), V13 (= V19), D38 (vs. gap), S39 (≠ A49), K57 (vs. gap), C85 (≠ L84), T86 (≠ I85), P87 (≠ G86), A112 (= A109), N113 (= N110), K114 (= K111), A139 (= A136), E198 (= E196), S199 (≠ A197)
Sites not aligning to the query:
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 72% coverage: 9:321/434 of query aligns to 3:309/319 of 4xb1A
- active site: D211 (= D207), K215 (= K211)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A14), F10 (≠ L16), G11 (= G17), T12 (= T18), V13 (= V19), D39 (≠ A49), R40 (= R50), K57 (= K54), V91 (≠ L84), S92 (≠ I85), S93 (≠ G86), S114 (≠ A109), K116 (= K111), S141 (≠ A136), G295 (≠ A307), T300 (= T312)
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
28% identity, 72% coverage: 9:321/434 of query aligns to 3:309/319 of O58802
- F10 (≠ L16) binding NADPH
- T12 (= T18) binding NADPH
- V13 (= V19) binding NADPH
- R40 (= R50) binding NADPH; mutation to A: Increases activity with NADP.
- K57 (= K54) binding NADPH; mutation to A: Increases activity with NADP.
- S92 (≠ I85) binding NADPH
- S93 (≠ G86) binding NADPH
- S114 (≠ A109) binding NADPH
- K116 (= K111) binding NADPH
- E140 (= E135) binding Na(+)
- V143 (= V138) binding Na(+)
- A145 (≠ G140) binding Na(+)
- T147 (≠ I142) binding Na(+)
- G296 (= G308) binding NADPH
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
28% identity, 72% coverage: 9:321/434 of query aligns to 3:309/319 of 4xb2A
- active site: D211 (= D207), K215 (= K211)
- binding l-homoserine: A171 (≠ G166), S172 (≠ T167), D206 (= D202), K215 (= K211)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A14), F10 (≠ L16), G11 (= G17), T12 (= T18), V13 (= V19), R40 (= R50), V91 (≠ L84), S92 (≠ I85), S93 (≠ G86), S114 (≠ A109), N115 (= N110), K116 (= K111), S141 (≠ A136), G295 (≠ A307), T300 (= T312)
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
31% identity, 52% coverage: 9:234/434 of query aligns to 4:239/321 of 3jsaA
- active site: D212 (= D207), K216 (= K211)
- binding nicotinamide-adenine-dinucleotide: G10 (= G15), L11 (= L16), G12 (= G17), N13 (≠ T18), V14 (= V19), D42 (vs. gap), S43 (= S48), A90 (vs. gap), T91 (= T95), P92 (≠ V96), A117 (= A109), N118 (= N110), A144 (= A136)
Sites not aligning to the query:
O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
25% identity, 74% coverage: 9:331/434 of query aligns to 558:916/916 of O81852
Sites not aligning to the query:
- 441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
- 522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
- 524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 52% coverage: 4:230/434 of query aligns to 3:249/376 of O94671
- S201 (≠ D181) modified: Phosphoserine
1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
36% identity, 28% coverage: 100:219/434 of query aligns to 105:230/358 of 1tveA
Sites not aligning to the query:
1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
36% identity, 28% coverage: 100:219/434 of query aligns to 105:230/358 of 1q7gA
Sites not aligning to the query:
1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
36% identity, 28% coverage: 100:219/434 of query aligns to 105:230/358 of 1ebuD
Sites not aligning to the query:
1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
36% identity, 28% coverage: 100:219/434 of query aligns to 105:230/358 of 1ebfA
Sites not aligning to the query:
Query Sequence
>WP_012973715.1 NCBI__GCF_000010725.1:WP_012973715.1
MPNAQQAPLKIAVAGLGTVGAGVLKLLDTQASLIERRCGRRIEVVAVSARSKGKDRGVDL
SAVQWFDDPVAMAAQSGADLVVELIGGSEGPARDTVATAIETGKHVVTANKALLAVHGTG
IARKAEAGGLTVAFEAAVAGGIPIIKGLREGLAANGVSEIHGILNGTCNYILTEMRTTGR
DFADVLADAQALGYAEADPSFDVDGIDAAHKLAILASVAFGTAVDFASVFIEGIRQVSAV
DFDYADQLGFRIKLLGIARRTDAGIEQRVHPCMVPKTAPIASVDGVFNAVVAQADFADRV
LFVGRGAGAGPTASAVVADLIDIARGRSTPTFGVPAGLLGASTPSPMEARRGAYYVRLMV
KDRPGVIADIAAAMRDQGVSMEQFLQRGRSPDEGVPVVLTTHDTDEASMRRALATIAASD
TVLEPPRMIRIEQF
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory