SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012974696.1 NCBI__GCF_000010725.1:WP_012974696.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

Q96PE7 Methylmalonyl-CoA epimerase, mitochondrial; DL-methylmalonyl-CoA racemase; EC 5.1.99.1 from Homo sapiens (Human) (see paper)
65% identity, 99% coverage: 2:134/134 of query aligns to 45:176/176 of Q96PE7

query
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Q96PE7

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H50 (= H7) binding Co(2+)
R104 (≠ E61) to L: in dbSNP:rs6748672
H122 (= H79) binding Co(2+)
E172 (= E130) binding Co(2+)

6qh4C Crystal structure of human methylmalonyl-coa epimerase (mcee) p.Arg143cys variant
63% identity, 100% coverage: 1:134/134 of query aligns to 8:138/138 of 6qh4C

query
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6qh4C

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active site: H14 (= H7), V50 (= V43), E61 (= E54), H86 (= H79), E134 (= E130)
binding cobalt (ii) ion: H14 (= H7), H86 (= H79), E134 (= E130)

3oa4A Crystal structure of hypothetical protein bh1468 from bacillus halodurans c-125
37% identity, 96% coverage: 4:131/134 of query aligns to 4:129/138 of 3oa4A

query
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active site: H7 (= H7), I43 (≠ V43), E54 (= E54), H78 (= H79), E128 (= E130)
binding zinc ion: H7 (= H7), E54 (= E54), H78 (= H79), E128 (= E130)

6wfhA Streptomyces coelicolor methylmalonyl-coa epimerase substrate complex (see paper)
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/139 of 6wfhA

query
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6wfhA

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active site: H7 (= H7), E43 (≠ V43), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H7), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding (3S,5R,9R,19E)-1-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-4-hydroxy-3-(phosphonooxy)tetrahydrofuran-2-yl]-3,5,9,19-tetrahydroxy-8,8,20-trimethyl-10,14-dioxo-2,4,6-trioxa-18-thia-11,15-diaza-3,5-diphosphahenicos-19-en-21-oic acid 3,5-dioxide (non-preferred name): H7 (= H7), Q39 (≠ H39), Q60 (≠ E54), A70 (≠ P64), K73 (≠ G67), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

6xbqA Streptomyces coelicolor methylmalonyl-coa epimerase in complex with carboxy-carba(dethia)-coa
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/144 of 6xbqA

query
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6xbqA

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active site: H7 (= H7), E43 (≠ V43), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding carboxymethyldethia coenzyme *a: Q39 (≠ H39), Q60 (≠ E54), A70 (≠ P64), K73 (≠ G67), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128)
binding cobalt (ii) ion: H7 (= H7), Q60 (≠ E54), H84 (= H79), E134 (= E130)

6wfiA Methylmalonyl-coa epimerase in complex with 2-nitronate-propionyl-coa (see paper)
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/144 of 6wfiA

query
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active site: H7 (= H7), E43 (≠ V43), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H7), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H7), Q39 (≠ H39), Q60 (≠ E54), A70 (≠ P64), K73 (≠ G67), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), G114 (= G110), S115 (≠ A111), I120 (≠ V116), F122 (= F118), P125 (= P121), L132 (= L128), E134 (= E130)

6wf7A Methylmalonyl-coa epimerase in complex with methylmalonyl-coa and nh4+ (see paper)
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/144 of 6wf7A

query
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active site: H7 (= H7), E43 (≠ V43), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding (S)-Methylmalonyl-Coenzyme A: Q39 (≠ H39), E43 (≠ V43), Q60 (≠ E54), A70 (≠ P64), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), G114 (= G110), S115 (≠ A111), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)
binding methylmalonyl-coenzyme a: Q39 (≠ H39), Q60 (≠ E54), A70 (≠ P64), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), G114 (= G110), S115 (≠ A111), I120 (≠ V116), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

6wf6A Streptomyces coelicolor methylmalonyl-coa epimerase (see paper)
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/141 of 6wf6A

query
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6wf6A

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H

E

P

P

F

T

G

R

E

E

T

D

S

S

P

A

V

V

A

G

G

K

F

W

L

L

E

A

K

K

N

N

P

G

S

E

G

G

G

-

I

V

H

H

H
|
H

I

I

C

A

Y

F

E

G

V

T

D

A

D

D

I

V

L

D

A

A

A

D

R

A

D

A

R

D

L

I

K

R

A

D

Q

K

G

G

A

V

R

R

V

V

L

L

G

Y

D

D

G

-

E

E

P

P

K

R

I

R

G

G

A

S

H

M

D

G

K

S

P

R

V

I

L

T

F

F

L

L

H

H

P

P

K

K

D

D

F

C

C

H

G

G

T

V

L

L

V

T

E
|
E

L

L

active site: H7 (= H7), E43 (≠ V43), Q60 (≠ E54), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H7), Q60 (≠ E54), H84 (= H79), E134 (= E130)

6xbtA Streptomyces coelicolor methylmalonyl-coa epimerase (q60a) in complex with 2-nitronate-propionyl-coa
34% identity, 96% coverage: 4:131/134 of query aligns to 4:135/140 of 6xbtA

query
sites
6xbtA

K

R

L

I

N

D

H
|
H

V

I

A

G

I

I

V

A

V

C

P

H

D

D

L

L

P

D

A

A

A

T

T

V

A

E

L

F

Y

Y

R

R

D

A

T

T

L

Y

G

G

A

F

A

E

V

V

S

F

Q

H

P

T

V

E

D

V

L

N

P

E

P

E

H
x
Q

G

G

V

V

T

R

V
x
E

V

A

F

M

V

L

T

K

L

I

P

N

N

D

T

T

K

S

-

D

-

G

-

G

-

A

-

S

-

Y

I

L

E
x
A

L

L

L

L

H

E

P

P

F

T

G

R

E

E

T

D

S

S

P
x
A

V

V

A

G

G
x
K

F
x
W

L

L

E

A

K
|
K

N

N

P

G

S

E

G

G

G

-

I

V

H
|
H

H
|
H

I

I

C

A

Y

F

E

G

V

T

D

A

D

D

I

V

L

D

A

A

A

D

R

A

D

A

R

D

L

I

K

R

A

D

Q

K

G

G

A

V

R

R

V

V

L
|
L

G

Y

D

D

G

-

E

E

P

P

K

R

I

R

G
|
G

A
x
S

H

M

D

G

K

S

P

R

V

I

L

T

F
|
F

L

L

H

H

P
|
P

K
|
K

D

D

F

C

C

H

G

G

T

V

L
|
L

V

T

E
|
E

L

L

active site: H7 (= H7), E43 (≠ V43), A60 (≠ E54), H84 (= H79), E134 (= E130)
binding cobalt (ii) ion: H7 (= H7), H84 (= H79), E134 (= E130)
binding coenzyme a: Q39 (≠ H39), A70 (≠ P64), K73 (≠ G67), W74 (≠ F68), K77 (= K71), H83 (= H78), L107 (= L102), F122 (= F118), P125 (= P121), K126 (= K122), L132 (= L128)
binding [1-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-1-oxidanylidene-propan-2-ylidene]-bis(oxidanidyl)azanium: H7 (= H7), Q39 (≠ H39), A70 (≠ P64), K73 (≠ G67), W74 (≠ F68), H83 (= H78), H84 (= H79), L107 (= L102), G114 (= G110), S115 (≠ A111), P125 (= P121), K126 (= K122), L132 (= L128), E134 (= E130)

2qh0A Crystal structure of a glyoxalase from clostridium acetobutylicum
27% identity, 96% coverage: 4:131/134 of query aligns to 3:126/129 of 2qh0A

query
sites
2qh0A

K

K

L

V

N

H

H
|
H

V

I

A

G

I

Y

V

A

V

V

P

K

D

N

L

I

P

D

A

S

A

A

T

L

A

K

L

K

Y

F

R

K

D

R

T

L

L

G

G

Y

A

V

A

E

V

E

S

S

Q

E

P

V

V

V

D

R

L

D

P

E

P

V

H

R

G

K

V

V

T

Y

V

I

V

Q

F

F

V

V

T

I

L

N

P

G

N

G

T

Y

K

R

I

V

E
|
E

L

L

L

V

H

A

P

P

F

D

G

G

E

E

T

D

S

S

P

P

V

I

A

N

G

K

F

T

L

I

E

K

K

K

N

G

P

S

S

T

G

P

G

-

I

-

H

Y

H
|
H

I

I

C

C

Y

Y

E

E

V

V

D

E

D

D

I

I

L

Q

A

K

A

S

R

I

D

E

R

E

L

M

K

S

A

Q

Q

I

G

G

A

Y

R

T

V

L

L

F

G

K

D

K

G

A

E

E

P

I

K

A

I

P

G

A

A

I

H

D

D

N

K

R

P

K

V

V

L

A

F

F

L

L

H

F

P

S

K

T

D

D

F

I

C

-

G

-

T

G

L

L

V

I

E
|
E

L

L

binding zinc ion: H6 (= H7), E53 (= E54), H76 (= H79), E125 (= E130)

Query Sequence

>WP_012974696.1 NCBI__GCF_000010725.1:WP_012974696.1
MIGKLNHVAIVVPDLPAATALYRDTLGAAVSQPVDLPPHGVTVVFVTLPNTKIELLHPFG
ETSPVAGFLEKNPSGGIHHICYEVDDILAARDRLKAQGARVLGDGEPKIGAHDKPVLFLH
PKDFCGTLVELEQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory