SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012975077.1 NCBI__GCF_000010725.1:WP_012975077.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8DLI5 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermosynechococcus vestitus (strain NIES-2133 / IAM M-273 / BP-1) (see paper)
39% identity, 93% coverage: 6:272/286 of query aligns to 5:265/485 of Q8DLI5

query
sites
Q8DLI5

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R6 (= R7) binding L-glutamate
Y192 (= Y199) binding L-glutamate

2cfoA Non-discriminating glutamyl-tRNA synthetase from thermosynechococcus elongatus in complex with glu (see paper)
39% identity, 93% coverage: 6:272/286 of query aligns to 4:264/484 of 2cfoA

query
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2cfoA

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active site: S9 (= S11), L239 (= L247), K250 (= K258)
binding glutamic acid: R5 (= R7), Y191 (= Y199), R209 (= R217), H213 (≠ L221)

8i9iA Glutamyl-tRNA synthetase from escherichia coli bound to glutamate and zinc
33% identity, 95% coverage: 4:275/286 of query aligns to 3:257/468 of 8i9iA

query
sites
8i9iA

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binding glutamic acid: R6 (= R7), A8 (= A9), P9 (= P10), S10 (= S11), R199 (= R217), H203 (≠ L221)
binding zinc ion: C100 (= C101), Y121 (= Y130), C125 (= C134)

P04805 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Escherichia coli (strain K12) (see 4 papers)
33% identity, 95% coverage: 4:275/286 of query aligns to 3:257/471 of P04805

query
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P04805

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C98 (= C99) mutation to S: 10-fold decrease in activity. Strong decrease in zinc content.
C100 (= C101) mutation to S: Loss of activity. Strong decrease in zinc content.; mutation to Y: Does not prevent zinc binding. Reduces only 2-fold the binding affinity for tRNA(Glu), but reduces more than 10-fold the affinity for glutamate in the presence of tRNA(Glu).
C125 (= C134) mutation to S: Loss of activity. Strong decrease in zinc content.
H127 (= H136) mutation to Q: 10-fold decrease in activity. Strong decrease in zinc content.
H129 (≠ S138) mutation to Q: No change in activity or in zinc content.
H131 (≠ R145) mutation to Q: No change in activity or in zinc content.
H132 (≠ I146) mutation to Q: No change in activity or in zinc content.
C138 (≠ W152) mutation to S: No change in activity or in zinc content.
S239 (≠ A257) modified: Phosphoserine; mutation to D: Does not aminoacylate tRNA(Glu), not phosphorylated by HipA.

4g6zA Crystal structure of a glutamyl-tRNA synthetase glurs from burkholderia thailandensis bound to l-glutamate (see paper)
35% identity, 95% coverage: 6:278/286 of query aligns to 5:245/380 of 4g6zA

query
sites
4g6zA

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L

P

P

G

E

P

P

T

P

S

A

S

G

D

V

G

A

P

P

L

V

Y

-

P

-

G

-

T

-

C

L

R

R

H

F

R

R

S

N

P

P

Q

-

E

-

R

-

A

-

E

-

R

-

I

-

G

-

D

-

G

-

E

-

S

-

W

-

A

-

L

-

R

-

L

-

D

-

V

-

E

-

A

-

R

-

L

L

T

T

G

G

P

T

L

V

R

A

W

W

H

D

D

D

R

A

A

V

R

K

G

G

W

R

Q

V

Q

E

A

I

T

S

P

N

E

E

L

E

L

L

G

D

D

D

V

L

V

V

L

V

A

A

R

R

K

P

D

D

T

G

P

T

T

P

S

M

Y

Y

H

N

L

F

S

C

V

V

T

V

V

V

D

D

H

L

L

D

Q

M

G

G

V

I

T

T

L

H

V

V

T

I

R
|
R

G

G

E

D

D

D

L
x
H

F

V

F

N

A

N

T

T

H

P

L

R

H

Q

R

I

L

N

L

I

Q

L

A

R

L

A

L

L

G

G

Y

G

D

E

P

V

P

P

D

V

Y

Y

H

A

H

H

H

L

G

P

L
x
T

L

V

R

L

N

N

A

E

A

Q

G

G

E

E

R

K

L

M

A

S

K
|
K

R

R

D

H

R

G

A

A

Q

M

S

S

L

V

R

M

S

G

L

Y

R

R

E

D

E

A

G

G

R

Y

G

L

P

P

A

E

E

A

V

V

active site: S10 (= S11), T214 (≠ L247), K225 (= K258)
binding glutamic acid: R6 (= R7), A8 (= A9), S10 (= S11), E42 (= E43), R184 (= R217), H188 (≠ L221)

P27305 Glutamyl-Q tRNA(Asp) synthetase; Glu-Q-RSs; EC 6.1.1.- from Escherichia coli (strain K12) (see paper)
37% identity, 92% coverage: 2:265/286 of query aligns to 14:248/308 of P27305

query
sites
P27305

S

T

D

Q

L

Y

V

I

T

G

R

R

F

F

A

A

P

P

S

S

P

P

T

S

G

G

H

E

L

L

H

H

L

F

G

G

H

S

A

L

H

I

S

A

A

A

L

L

F

G

G

S

W

Y

T

L

T

Q

A

A

R

R

G

A

A

R

A

Q

G

G

R

R

F

W

L

L

L

V

R

R

I

I

E
|
E

D

D

I

I

D

D

P

P

N

P

R

R

C

E

R

V

P

P

E

G

F

A

E

A

R

E

D

T

L

I

I

L

E

R

D

Q

L

L

G

E

W

H

L

Y

G

G

L

L

D

H

W

W

D

D

G

G

P

D

V

V

R

L

R

W

Q

Q

S

S

D

Q

H

R

F

H

D

D

D

A

Y

Y

R

R

A

E

A

A

L

L

A

A

R

W

L

L

E

H

S

E

L

Q

G

G

V

L

L

S

Y

Y

P

Y

C

C

F

T

C

C

T

T

R

R

K

A

D

R

I

I

A

-

E

-

I

-

A

-

R

-

A

-

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

Q

S

S

D

I

G

G

P

G

L

I

Y

Y

P

D

G

G

T

H

C

C

R

R

-

V

-

L

H

H

R

H

S

G

P

P

Q

D

E

N

R

A

A

A

E

V

R

R

I

I

G

-

D

-

G

-

E

-

S

-

W

-

A

-

L

-

R

-

L

-

D

-

V

-

E

-

A

-

R

R

R

Q

L

Q

T

H

G

P

P

V

L

T

R

Q

W

F

H

T

D

D

R

Q

A

L

R

R

G

G

W

I

Q

I

Q

H

A

A

T

D

P

E

E

K

L

L

L

A

-

R

G

E

D

D

V

F

V

I

L

I

A

H

R

R

K

R

D

D

T

G

P

L

T

F

S

A

Y
|
Y

H

N

L

L

S

A

V

V

T

V

V

V

D

D

H

H

L

F

Q

Q

G

G

V

V

T

T

L

E

V

I

T

V

R
|
R

G

G

E

A

D

D

L

L

F

I

F

E

A

P

T

T

H

V

L

R

H

Q

R

I

L

S

L

L

Q

Y

A

Q

L

L

L

F

G

G

Y

W

D

K

P

V

P

P

D

D

Y

Y

H

I

H

H

H

L

G

P

L

L

L

A

R

L

N

N

A

P

A

Q

G

G

E

A

R

K

L

L

A

S

K

K

R

Q

D

N

R

H

A

A

Q

P

S

A

L

L

E55 (= E43) binding L-glutamate
Y182 (= Y199) binding L-glutamate
R200 (= R217) binding L-glutamate

2cv2A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu) and an enzyme inhibitor, glu-ams (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 2cv2A

query
sites
2cv2A

L

V

V

V

T

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G
|
G

H

T

A

A

H

Y

S
x
I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

P

R

N

A

R
|
R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A
x
V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R
|
R

G

G

W

V

Q

V

A
x
Y

T

D

P

N

E

Q

L
x
E

L

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

K
x
S

D

D

T

G

P

Y

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

F

L

F
x
V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L
|
L

R
|
R

N

N

A

P

A

D

G
x
K

E

T

R

K

L

I

A

S

K
|
K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L
|
L

A
x
M

G
|
G

F

F

active site: K246 (= K258)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R5 (= R7), A7 (= A9), S9 (= S11), G17 (= G19), I21 (≠ S23), E41 (= E43), Y187 (= Y199), R205 (= R217), A206 (≠ G218), E208 (≠ D220), W209 (≠ L221), L235 (= L247), L236 (= L248)
binding : S9 (= S11), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A153), R163 (= R175), Y168 (≠ A180), E172 (≠ L184), V177 (= V189), K180 (≠ R192), S181 (≠ K193), Y187 (= Y199), E207 (= E219), E208 (≠ D220), W209 (≠ L221), V211 (≠ F223), R237 (= R249), K241 (≠ G253), L272 (= L281), M273 (≠ A282), G274 (= G283)

Sites not aligning to the query:

binding : 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 432, 435, 442, 443, 444, 446, 447, 448

2cv1A Glutamyl-tRNA synthetase from thermus thermophilus in complex with tRNA(glu), atp, and an analog of l-glutamate: a quaternary complex
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 2cv1A

query
sites
2cv1A

L

V

V

V

T

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G
|
G

H
x
T

A

A

H

Y

S
x
I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

P

R

N

A

R
|
R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A
x
V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R
|
R

G

G

W

V

Q
x
V

Q

V

A

Y

T

D

P

N

E

Q

L
x
E

L

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

K
x
S

D

D

T

G

P

Y

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

F

L

F
x
V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L
|
L

R
|
R

N

N

A

P

A

D

G
x
K

E

T

R
x
K

L

I

A

S

K
|
K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A
x
M

G
|
G

F

F

active site: K246 (= K258)
binding adenosine-5'-triphosphate: P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ H20), I21 (≠ S23), R47 (= R49), A206 (≠ G218), W209 (≠ L221), L235 (= L247), L236 (= L248)
binding (4s)-4-amino-5-hydroxypentanoic acid: R5 (= R7), A7 (= A9), E41 (= E43), Y187 (= Y199), R205 (= R217), W209 (≠ L221)
binding : S9 (= S11), E41 (= E43), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A153), R163 (= R175), V166 (≠ Q178), E172 (≠ L184), V177 (= V189), K180 (≠ R192), S181 (≠ K193), Y187 (= Y199), E207 (= E219), E208 (≠ D220), W209 (≠ L221), V211 (≠ F223), R237 (= R249), K241 (≠ G253), K243 (≠ R255), M273 (≠ A282), G274 (= G283)

Sites not aligning to the query:

binding : 276, 282, 299, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

2cuzA Glutamyl-tRNA synthetase from thermus thermophilus in complex with l- glutamate (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 2cuzA

query
sites
2cuzA

L

V

V

V

T

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G

G

H

T

A

A

H

Y

S

I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

P

R

N

A

R

R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A

V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R

R

G

G

W

V

Q

V

A

Y

T

D

P

N

E

Q

L

E

L

I

G

P

D

D

V

V

L

L

A

L

R

K

K

S

D

D

T

G

P

Y

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R
|
R

G

A

E

E

D

E

L

W

F

L

F

V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L

L

R

R

N

N

A

P

A

D

G

K

E

T

R

K

L

I

A

S

K
|
K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A

M

G

G

F

F

active site: K246 (= K258)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y187 (= Y199), R205 (= R217)

1n78A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with tRNA(glu) and glutamol-amp. (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 1n78A

query
sites
1n78A

L

V

V

V

T

T

R
|
R

F

I

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G
|
G

H
x
T

A

A

H

Y

S
x
I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

P

R

N

A

R
|
R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A
x
V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R
|
R

G

G

W

V

Q
x
V

Q

V

A
x
Y

T

D

P

N

E

Q

L
x
E

L

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

K
x
S

D

D

T

G

P

Y

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V
x
N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R
|
R

G
x
A

E
|
E

D
x
E

L
x
W

F
x
L

F
x
V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L
|
L

R
|
R

N

N

A

P

A

D

G
x
K

E

T

R

K

L

I

A

S

K
|
K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A
x
M

G
|
G

F

F

active site: K246 (= K258)
binding glutamol-amp: R5 (= R7), A7 (= A9), P8 (= P10), S9 (= S11), G17 (= G19), T18 (≠ H20), I21 (≠ S23), E41 (= E43), Y187 (= Y199), N191 (≠ V203), R205 (= R217), A206 (≠ G218), E208 (≠ D220), W209 (≠ L221), L235 (= L247), L236 (= L248)
binding : S9 (= S11), T43 (≠ I45), D44 (= D46), R47 (= R49), V145 (≠ A153), R163 (= R175), V166 (≠ Q178), Y168 (≠ A180), E172 (≠ L184), V177 (= V189), K180 (≠ R192), S181 (≠ K193), Y187 (= Y199), E207 (= E219), E208 (≠ D220), W209 (≠ L221), L210 (≠ F222), V211 (≠ F223), R237 (= R249), K241 (≠ G253), M273 (≠ A282), G274 (= G283)

Sites not aligning to the query:

binding : 282, 297, 303, 304, 309, 312, 319, 357, 358, 417, 427, 432, 435, 442, 443, 444, 446, 447, 448

1j09A Crystal structure of thermus thermophilus glutamyl-tRNA synthetase complexed with atp and glu (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 1j09A

query
sites
1j09A

L

V

V

V

T

T

R
|
R

F

I

A
|
A

P

P

S
|
S

P

P

T

T

G

G

H

D

L

P

H
|
H

L

V

G

G

H

T

A

A

H

Y

S

I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E
|
E

D

D

I

T

D

D

P

R

N

A

R

R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A

V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R

R

G

G

W

V

Q

V

A

Y

T

D

P

N

E

Q

L

E

L

I

G

P

D

D

V

V

L

L

A

L

R

K

K

S

D

D

T

G

P

Y

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V
x
N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R
|
R

G

A

E

E

D
x
E

L
x
W

F

L

F

V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L
|
L

R

R

N

N

A

P

A

D

G

K

E

T

R
x
K

L
x
I

A
x
S

K
|
K

R
|
R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A

M

G

G

F

F

active site: K246 (= K258)
binding adenosine-5'-triphosphate: H15 (= H17), E208 (≠ D220), L235 (= L247), L236 (= L248), K243 (≠ R255), I244 (≠ L256), S245 (≠ A257), K246 (= K258), R247 (= R259)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y187 (= Y199), N191 (≠ V203), R205 (= R217), W209 (≠ L221)

P27000 Glutamate--tRNA ligase; Glutamyl-tRNA synthetase; GluRS; EC 6.1.1.17 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of P27000

query
sites
P27000

L

V

V

V

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G

G

H

T

A

A

H

Y

S

I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E

E

D

D

I

T

D

D

P

R

N

A

R

R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R

P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A

V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R

R

G

G

W

V

Q

V

A

Y

T

D

P

N

E

Q

L

E

L

I

G

P

D

D

V

V

L

L

A

L

R

K

K

S

D

D

T

G

P

Y

T

P

S

T

Y

Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R

R

G

A

E

E

D

E

L

W

F

L

F

V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L

L

R

R

N

N

A

P

A

D

G

K

E

T

R

K

L

I

A

S

K

K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A

M

G

G

F

F

Sites not aligning to the query:

358 R→Q: Reduces affinity for tRNA and abolishes the ability to discriminate between tRNA(Glu) and tRNA(Gln).

1g59A Glutamyl-tRNA synthetase complexed with tRNA(glu). (see paper)
35% identity, 98% coverage: 4:284/286 of query aligns to 2:275/468 of 1g59A

query
sites
1g59A

L

V

V

V

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G

G

H

T

A

A

H

Y

S

I

A

A

L

L

F

F

G

N

W

Y

T

A

T

W

A

A

R

R

G

R

A

N

A

G

G

G

R

R

F

F

L

I

L

V

R

R

I

I

E

E

D

D

I

T

D
|
D

P
x
R

N
x
A

R
|
R

C

Y

R

V

P

P

E

G

F

A

E

E

R

E

D

R

L

I

I

L

E

A

D

A

L

L

G

K

W

W

L

L

G

G

L

L

D

S

W

Y

D

D

-

E

-

G

-

P

-

D

-

V

-

A

-

P

-

T

G

G

P

P

V

Y

R

R

R

-

Q

Q

S

S

D

E

H

R

F

L

D

P

D

L

Y

Y

R

Q

A

K

A

Y

L

A

A

E

R

E

L

L

E

L

S

K

L

R

G

G

V

W

L

A

Y

Y

P

R

C

A

F

F

C

E

T

T

R
x
P

K

E

D

E

I

L

A

E

A

-

E

Q

I

I

A

R

R

K

A

E

A

K

A

G

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

Y

P

D

G

G

T

R

C

A

R

R

H

N

R

I

S

P

P

P

Q

E

E

E

R

A

A

E

E

E

R

R

I

A

G

R

D

R

G

G

E

E

S

P

W

H

A
x
V

L

I

R

R

L

L

D

K

V

V

E

P

A

R

A

P

R

-

L

-

T

-

G

G

P

T

L

T

R

E

W

V

H

K

D

D

R

E

A

L

R
|
R

G

G

W

V

Q
x
V

Q

V

A

Y

T

D

P

N

E

Q

L
x
E

L

I

G

P

D

D

V

V

V
|
V

L

L

A

L

R
x
K

K
x
S

D
|
D

T

G

P

Y

T

P

S

T

Y

Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

V

T

T

L

D

V

V

T

I

R

R

G

A

E
|
E

D
x
E

L

W

F

L

F

V

A

S

T

T

H

P

L

I

H

H

R

V

L

L

Q

Y

A

R

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

R

Y

F

H

Y

H

H

H

M

G

P

L

L

R
|
R

N

N

A

P

A

D

G
x
K

E
x
T

R
x
K

L

I

A

S

K

K

R

R

D

K

R

S

A

H

Q

T

S

S

L

L

R

D

S

W

L

Y

R

K

E

A

E

E

G

G

R

F

G

L

P

P

A

E

E

A

V

L

R

R

-

N

-

Y

-

L

A

C

L

L

A
x
M

G
|
G

F

F

binding : D44 (= D46), R45 (≠ P47), A46 (≠ N48), R47 (= R49), P109 (≠ R103), V145 (≠ A153), R163 (= R175), V166 (≠ Q178), E172 (≠ L184), V177 (= V189), K180 (≠ R192), S181 (≠ K193), D182 (= D194), E207 (= E219), E208 (≠ D220), R237 (= R249), K241 (≠ G253), T242 (≠ E254), K243 (≠ R255), M273 (≠ A282), G274 (= G283)

Sites not aligning to the query:

binding : 282, 299, 300, 303, 304, 309, 312, 319, 357, 358, 417, 426, 427, 432, 435, 442, 443, 444, 445, 446, 447, 448

4a91A Crystal structure of the glutamyl-queuosine trnaasp synthetase from e. Coli complexed with l-glutamate (see paper)
37% identity, 90% coverage: 2:259/286 of query aligns to 2:230/290 of 4a91A

query
sites
4a91A

S

T

D

Q

L

Y

V

I

T

G

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

S

G

G

H

E

L

L

H

H

L

F

G

G

H

S

A

L

H

I

S

A

A

A

L

L

F

G

G

S

W

Y

T

L

T

Q

A

A

R

R

G

A

A

R

A

Q

G

G

R

R

F

W

L

L

L

V

R

R

I

I

E
|
E

D

D

I

I

D

D

P

P

N

P

R

R

C

E

R

V

P

P

E

G

F

A

E

A

R

E

D

T

L

I

I

L

E

R

D

Q

L

L

G

E

W

H

L

Y

G

G

L

L

D

H

W

W

D

D

G

G

P

D

V

V

R

L

R

W

Q

Q

S

S

D

Q

H

R

F

H

D

D

D

A

Y

Y

R

R

A

E

A

A

L

L

A

A

R

W

L

L

E

H

S

E

L

Q

G

G

V

L

L

S

Y

Y

P

Y

C
|
C

F

T

C
|
C

T

T

R

R

K

A

D

R

I

I

A

-

E

-

I

-

A

-

R

-

A

-

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

Q

S

S

D

I

G

G

P

G

L

I

Y
|
Y

P

D

G

G

T

H

C
|
C

R

R

-

V

-

L

H

H

R

H

S

G

P

P

Q

D

E

N

R

A

A

A

E

V

R

R

I

I

G

-

D

-

G

-

E

-

S

-

W

-

A

-

L

-

R

-

L

-

D

-

V

-

E

-

A

-

R

R

R

Q

L

Q

T

H

G

P

P

V

L

T

R

Q

W

F

H

T

D

D

R

Q

A

L

R

R

G

G

W

I

Q

I

Q

H

A

A

T

D

P

E

E

K

L

L

L

A

-

R

G

E

D

D

V

F

V

I

L

I

A

H

R

R

K

R

D

D

T

G

P

L

T

F

S

A

Y
|
Y

H

N

L

L

S

A

V

V

T

V

V

V

D

D

H

H

L

F

Q

Q

G

G

V

V

T

T

L

E

V

I

T

V

R
|
R

G

G

E

A

D

D

L
|
L

F

I

F

E

A

P

T

T

H

V

L

R

H

Q

R

I

L

S

L

L

Q

Y

A

Q

L

L

L

F

G

G

Y

W

D

K

P

V

P

P

D

D

Y

Y

H

I

H

H

H

L

G

P

L

L

L

A

R

L

N

N

A

P

A

Q

G

G

E

A

R

K

L

L

A

S

K
|
K

R

Q

active site: S11 (= S11), K229 (= K258)
binding glutamic acid: R7 (= R7), A9 (= A9), S11 (= S11), E43 (= E43), Y170 (= Y199), R188 (= R217), L192 (= L221)
binding zinc ion: C99 (= C99), C101 (= C101), Y113 (= Y130), C117 (= C134)

3al0C Crystal structure of the glutamine transamidosome from thermotoga maritima in the glutamylation state. (see paper)
30% identity, 93% coverage: 6:272/286 of query aligns to 105:349/564 of 3al0C

query
sites
3al0C

T

V

R
|
R

F

F

A
|
A

P
|
P

S
|
S

P

P

T

T

G

G

H

H

L

L

H

H

L

V

G
|
G

H

G

A

A

H

R

S
x
T

A

A

L

L

F

F

G

N

W

W

T

M

T

F

A

A

R

R

G

K

A

E

A

G

G

G

R

K

F

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I
x
T

D
|
D

P

T

N

E

R
|
R

C

S

R

S

P

R

E

E

F

Y

E

E

R

Q

D

Q

L

I

I

L

E

E

D

S

L

L

G

R

W

W

L

C

G

G

L

L

D

D

W

W

D

D

-

E

-

G

-

P

-

D

-

I

-

G

-

D

-

F

G

G

P

P

V

Y

R

-

R

R

Q

Q

S

S

D

E

H

R

F

L

D

E

D

I

Y

Y

R

R

A

E

A

Y

L

A

A

E

R

K

L

L

E

V

S

E

L

D

G

K

V

R

L

A

Y

Y

P

Y

C

V

F

V

C
x
Y

T

D

R

K

K

E

D

D

I
x
P

A

S

A

K

E

E

I

L

A

F

R

T

A

T

A

Y

A

E

A

Y

P

P

H

H

G

E

P

Y

G

K

P

E

T

K

S

G

S

-

D

-

G

-

P

-

L

-

Y

H

P

P

G

V

T

T

C

I

R

K

H

F

R

K

S

V

P

-

Q

-

E

-

R

-

A

-

E

-

R

-

I

-

G

-

D

-

G

-

E

-

S

-

W

-

A

-

L

-

R

-

L

-

D

-

V

-

E

-

A

-

R

-

L

L

T

P

G

G

P

K

L

T

R

S

W

F

H

E

D

D

R

L

A

L

R
x
K

G

G

W

Y

Q
x
M

Q

E

A

F

T

D

P

N

E

S

L

T

L

L

G

E

D

D

V

F

V
x
I

L

I

A

M

R
x
K

K
x
S

D

N

T

G

P

F

T

P

S

T

Y
|
Y

H

N

L

F

S

A

V

V

T

V

V

V

D

D

H

H

L

L

Q

M

G

R

V

I

T

S

L

H

V

V

T

F

R
|
R

G
|
G

E

E

D
|
D

L
x
H

F
x
L

F
x
S

A
x
N

T

T

H

P

L
x
K

H

Q

R

L

L

M

L

I

Q

Y

A

E

L

A

L

F

G

G

Y

W

D

E

P

A

P

P

D

V

Y

F

H

M

H

H

H

I

G

P

L
|
L

L
x
I

R

L

N

G

A

S

A

D

G
x
R

E

T

R
x
P

L
|
L

A

S

K
|
K

R

R

D

H

R

G

A

A

Q

T

S

S

L

V

R

E

S

H

L

F

R

R

E

R

E

E

G

G

active site: S110 (= S11), K335 (= K258)
binding o5'-(l-glutamyl-sulfamoyl)-adenosine: R106 (= R7), A108 (= A9), P109 (= P10), G118 (= G19), T122 (≠ S23), E142 (= E43), Y276 (= Y199), R294 (= R217), G295 (= G218), D297 (= D220), H298 (≠ L221), L324 (= L247), I325 (≠ L248), L333 (= L256)
binding : T144 (≠ I45), D145 (= D46), R148 (= R49), Y208 (≠ C101), P213 (≠ I106), K252 (≠ R175), M255 (≠ Q178), I266 (≠ V189), K269 (≠ R192), S270 (≠ K193), Y276 (= Y199), D297 (= D220), H298 (≠ L221), L299 (≠ F222), S300 (≠ F223), N301 (≠ A224), K304 (≠ L227), R330 (≠ G253), P332 (≠ R255)

Sites not aligning to the query:

binding : 363, 364, 365, 370, 387, 389, 391, 392, 397, 400, 407, 446, 447, 453, 457, 509, 520, 524, 527, 535, 536, 538, 539

8vc5A Crystal structure of glutamyl-tRNA synthetase glurs from pseudomonas aeruginosa (zinc bound)
33% identity, 100% coverage: 1:286/286 of query aligns to 1:279/488 of 8vc5A

query
sites
8vc5A

M

M

S

T

D

T

L

V

V

R

T

T

R

R

F

I

A

A

P

P

S

S

P

P

T

T

G

G

H

D

L

P

H

H

L

V

G

G

H

T

A

A

H

Y

S

I

A

A

L

L

F

F

G

N

W

L

T

C

T

F

A

A

R

R

G

Q

A

H

A

G

G

G

R

Q

F

F

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

P

Q

N

L

R

R

C

S

R

T

P

R

E

E

F

S

E

E

R

Q

D

Q

L

I

I

Y

E

D

D

A

L

L

G

R

W

W

L

L

G

G

L

I

D

E

W

W

D

D

-

E

-

G

-

P

-

D

-

V

-

G

-

P

-

H

G

G

P

P

V

Y

R

-

R

R

Q

Q

S

S

D

E

H

R

F

G

D

H

D

I

Y

Y

R

K

A

K

A

Y

L

S

A

D

R

E

L

L

E

V

S

E

L

K

G

G

V

H

L

A

Y

F

P

T

C
|
C

F

F

C
|
C

T

T

R

P

K

E

D

R

I

L

A

D

A

A

E

V

I

R

A

A

R

E

A

Q

A

M

A

A

A

-

P

-

H

-

G

-

P

-

G

-

P

-

T

-

S

R

S

K

D

E

G

T

P

P

L

R

Y
|
Y

P

D

G

G

T

H

C
|
C

R

M

H

H

R

L

S

P

P

K

Q

D

E

E

R

V

A

Q

E

R

R

R

I

L

G

A

D

A

G

G

E

E

S

S

W

H

A

V

L

T

R

R

L

M

D

K

V

V

E

P

A

T

A

E

R

G

R

V

L

C

T

V

G

V

P

P

L

-

R

-

W

-

H

-

D

D

R

M

A

L

R

R

G

G

W

D

Q

V

Q

E

A

I

T

P

P

W

E

D

L

R

L

M

G

D

D

M

V

Q

V

V

L

L

A

M

R

K

K

A

D

D

T

G

P

L

T

P

S

T

Y

Y

H

F

L

L

S

A

V

N

T

V

V

V

D

D

H

H

L

L

Q

M

G

G

V

I

T

T

L

H

V

V

T

L

R

R

G

G

E

E

D

E

L

W

F

L

F

P

A

S

T

A

H

P

L

K

H

L

R

I

L

K

L

L

Q

Y

A

E

L

Y

L

F

G

G

Y

W

D

E

P

Q

P

P

D

Q

Y

L

H

C

H

Y

H

M

G

P

L

L

R

R

N

N

A

P

A

D

G

K

E

S

R

K

L

L

A

S

K

K

R

R

D

K

R

N

A

P

Q

T

S

S

L

I

R

T

S

F

L

Y

R

E

E

R

E

M

G

G

R

Y

G

L

P

P

A

-

E

-

V

-

R

Q

A

A

L

L

A

L

G

N

F

Y

P

L

G

G

binding zinc ion: C107 (= C99), C109 (= C101), Y130 (= Y130), C134 (= C134)

4griB Crystal structure of a glutamyl-tRNA synthetase glurs from borrelia burgdorferi bound to glutamic acid and zinc (see paper)
31% identity, 95% coverage: 6:278/286 of query aligns to 4:273/485 of 4griB

query
sites
4griB

T

V

R
|
R

F

Y

A
|
A

P

P

S
|
S

P

P

T

T

G

G

H

L

L

Q

H

H

L

I

G

G

H

G

A

I

H

R

S

T

A

A

L

L

F

F

G

N

W

Y

T

F

A

A

R

K

G

S

A

C

A

G

G

G

R

K

F

F

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

P

Q

N

S

R

R

C

Y

R

S

P

P

E

E

F

A

E

E

R

N

D

D

L

L

I

Y

E

S

D

S

L

L

G

K

W

W

L

L

G

G

L

I

D

S

W

F

D

D

-

E

G

G

P

P

V

V

-

V

-

G

-

D

-

Y

-

A

-

P

R

Y

R

V

Q

Q

S

S

D

Q

H

R

F

S

D

A

D

I

Y

Y

R

K

A

Q

A

Y

L

A

A

K

R

Y

L

L

E

I

S

E

L

S

G

G

V

H

L

A

Y

Y

P

Y

C
|
C

F

Y

C
|
C

T

S

-

P

-

E

R

R

K

L

D

E

I

R

A

I

A

K

E

K

I

I

A

Q

R

N

A

I

A

N

A

K

A

M

P

P

H

P

G

G

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y
|
Y

P

D

G

R

T

H

C
|
C

R

R

H

N

R

L

S

S

P

N

Q

E

E

E

R

V

A

E

E

N

R

A

I

L

G

I

D

K

G

K

E

I

S

K

W

P

A

V

L

V

R

R

L

F

D

K

V

I

E

P

A

-

R

-

L

L

T

E

G

G

P

D

L

T

R

S

W

F

H

D

D

D

R

I

A

L

R

L

G

G

-

R

-

I

-

T

W

W

Q

-

A

A

T

N

P

K

E

D

L

I

L

S

G

P

D

D

V

P

V

V

L

I

A

L

R

K

K

S

D

D

T

G

P

L

T

P

S

T

Y
|
Y

H

H

L

L

S

A

V

N

T

V

V

V

D

D

H

Y

L

L

Q

M

G

K

V

I

T

T

L

H

V

V

T

L

R
|
R

G

A

E

Q

D

E

L
x
W

F

V

F

S

A

S

T

G

H

P

L

L

H

H

R

V

L

L

Q

Y

A

K

L

A

L

F

G

K

Y

W

D

K

P

P

D

I

Y

Y

H

C

H

H

H

L

G

P

L

M

L

V

R

M

N

G

A

N

A

D

G

G

E

Q

R

K

L

L

A

S

K
|
K

R

R

D

H

R

G

A

S

Q

T

S

A

L

L

R

R

S

Q

L

F

R

I

E

E

E

D

G

G

R

Y

G

L

P

P

A

E

E

A

V

I

active site: S9 (= S11), K253 (= K258)
binding glutamic acid: R5 (= R7), A7 (= A9), S9 (= S11), E41 (= E43), Y194 (= Y199), R212 (= R217), W216 (≠ L221)
binding zinc ion: C105 (= C99), C107 (= C101), Y128 (= Y130), C132 (= C134)

6brlA Crystal structure of a glutamate tRNA ligase from elizabethkingia meningosepticum ccug26117 in complex with its amino acid (see paper)
31% identity, 89% coverage: 6:260/286 of query aligns to 5:263/502 of 6brlA

query
sites
6brlA

T

V

R
|
R

F

F

A
|
A

P

P

S
|
S

P

P

T

T

G

G

H

P

L

L

H

H

L

L

G

G

H

G

A

V

H

R

S

T

A

A

L

L

F

Y

G

D

W

Y

T

L

T

F

A

A

R

K

G

H

A

N

A

G

G

G

R

D

F

F

L

I

L

L

R

R

I

I

E
|
E

D

D

I

T

D

D

P

T

N

Q

R

R

C

Y

R

V

P

P

E

G

F

S

E

E

R

E

D

Y

L

I

I

M

E

E

D

A

L

L

G

E

W

W

L

I

G

G

L

M

D

V

W

P

D

D

-

E

-

S

-

P

-

K

-

H

-

G

G

G

P

P

V

Y

-

A

-

P

R

Y

R

R

Q

Q

S

S

D

E

H

R

F

R

D

D

D

I

Y

Y

R

D

A

R

A

Y

L

T

A

E

R

Q

L

I

E

L

S

K

L

T

G

D

V

Y

L

A

Y

Y

P

L

C

A

F

F

C

D

T

T

R

P

K

E

D

E

I

L

A

-

E

D

I

Q

A

I

R

R

A

A

A

E

A

F

A

E

P

A

H

R

G

G

P

-

G

-

P

-

T

-

S

-

D

D

G

V

P

F

L

A

Y

Y

P

N

G

Y

T

E

C

T

R

R

H

N

R

R

-

L

-

R

-

N

-

S

-

I

-

S

S

L

P

P

Q

E

E

E

R

E

A

V

E

K

R

K

I

L

G

L

D

E

G

E

E

K

S

T

-

P

W

Y

A

V

L

I

R

R

L

F

D

K

V

M

E

P

A

L

A

D

R

R

R

I

L

I

T

N

G

-

P

-

L

-

R

-

W

L

H

N

D

D

R

I

A

I

R

R

G

G

W

K

Q

F

Q

S

A

V

T

N

P

T

E

N

L

T

L

L

G

D

D

D

V

K

V

V

L

L

A

V

R

K

K

N

D

D

T

G

P

M

T

P

S

T

Y
|
Y

H

H

L

F

S

A

V
x
N

T

I

V

I

D

D

H

H

L

E

Q

M

G

K

V

I

T

T

L

H

V

V

T

I

R
|
R

G

G

E

E

D

E

L
x
W

F

L

F

P

A

S

T

M

H

A

L

L

H

H

R

V

L

L

Q

Y

A

E

L

A

L

M

G

G

Y

W

D

D

P

A

P

P

D

E

Y

F

H

A

H

H

H

L

G

S

L

L

L

I

R

L

N

K

A

P

A

E

G

G

E

K

-

G

R

K

L

L

A

S

K
|
K

R

R

D

D

active site: K261 (= K258)
binding glutamic acid: R6 (= R7), A8 (= A9), S10 (= S11), E42 (= E43), Y201 (= Y199), N205 (≠ V203), R219 (= R217), W223 (≠ L221)

3aiiA Archaeal non-discriminating glutamyl-tRNA synthetase from methanothermobacter thermautotrophicus (see paper)
31% identity, 98% coverage: 3:281/286 of query aligns to 10:273/455 of 3aiiA

query
sites
3aiiA

D

E

L

V

V

V

T

L

R

R

F

F

A

A

P

P

S
x
N

P

P

T

S

G

G

H

P

L

L

H

H

L

I

G

G

H

H

A

A

H

R

S

A

A

A

L

I

F

L

G

N

W

H

T

E

T

Y

A

A

R

R

G

K

A

Y

A

D

G

G

R

R

F

L

L

I

L

L

R

R

I

I

E

E

D

D

I

T

D

D

P

P

N

R

R

R

C

V

R

D

P

P

E

E

F

A

E

Y

R

D

D

M

L

I

I

P

E

A

D

D

L

L

G

E

W

W

L

L

G

G

L

V

D

E

W

W

D

D

G

E

P

T

V

V

R

-

R

I

Q

Q

S

S

D

D

H

R

F

M

D

E

D

T

Y

Y

R

Y

A

E

A

Y

L

T

A

E

R

K

L

L

E

I

S

E

L

R

G

G

V

G

L

A

Y

Y

P

V

C
|
C

F

T

C
|
C

T

-

R

R

K

P

D

E

I

E

A

F

A

R

E

E

I

L

A

K

R

N

A

R

A

G

A

E

A

A

P
x
C

H

H

G

-

P

-

G

-

P

-

T

-

S

-

D

-

G

-

P

-

L

-

Y

-

P

-

G

-

T

-

C
|
C

R

R

H

S

R

L

S

G

P

F

Q

R

E

E

R

N

A

L

E

Q

R

R

I

W

G

-

D

-

G

R

E

E

S

M

W

F

A

E

L

M

R

K

L

E

D

G

V

S

E

A

A

V

R

R

R

V

L

K

T

T

G

D

P

L

L

N

R

H

W

P

H

N

D

P

R

A

A

I

R

R

G

D

W

W

Q

V

Q

S

A

-

T

-

P

-

E

-

L

-

L

M

G

R

D

I

V

V

V

E

L

A

A

E

R

H

K

P

D

R

T

T

P

G

T

T

S

R

Y

Y

-

R

-

V

-

Y

-

P

-

M

H

N

L

F

S

S

V

V

T

A

V

V

D

D

H

H

L

L

Q

L

G

G

V

V

T

T

L

H

V

V

T

L

R

R

G

A

E

N

D

-

L

-

F

-

A

-

T

R

H

E

L

K

H

Q

R

E

L

Y

L

L

Q

Y

A

R

L

H

L

L

G

G

Y

W

D

E

P

P

D

E

Y

F

H

I

H

H

H

Y

G

G

L
x
R

L

L

R

K

N
x
T

A

S

A

G

-

A

-

R

-

E

-

G

-

I

-

L

-

R

G

G

E

E

R

Y

L

S

A

G

K

W

R

D

D

D

-

P

R

R

A

L

Q

G

S

T

L

L

R

R

S

A

L

I

R

A

E

R

G

G

R

I

G

R

P

P

A

E

E

A

V

I

R

R

A

K

L

L

active site: N18 (≠ S11), R231 (≠ L247), T234 (≠ N250)
binding zinc ion: C105 (= C99), C107 (= C101), C122 (≠ P117), C124 (= C134)

P00962 Glutamine--tRNA ligase; Glutaminyl-tRNA synthetase; GlnRS; EC 6.1.1.18 from Escherichia coli (strain K12) (see 7 papers)
38% identity, 32% coverage: 6:97/286 of query aligns to 30:121/554 of P00962

query
sites
P00962

T

T

R
|
R

F

F

A

P

P

P

S
x
E

P
|
P

T
x
N

G

G

H

Y

L

L

H
|
H

L
x
I

G
|
G

H
|
H

A
|
A

H
x
K

S
|
S

A

I

L

C

F

L

G

N

W

F

T

G

T

I

A

A

R

Q

G

D

A

Y

A

K

G

G

R

Q

F

C

L

N

L

L

R

R

I

F

E
x
D

D

D

I

T

D

N

P

P

N

V

R

K

C

E

R

D

P

I

E

E

F

Y

E

V

R

E

D

S

L

I

I

K

E

N

D

D

L

V

G

E

W

W

L

L

G

G

L

F

D

H

W

W

D

S

G

G

P

N

V

V

R

R

R

Y

Q

S

S

S

D

D

H

Y

F

F

D

D

D

Q

Y

L

R

H

A

A

A

Y

L

A

A

I

R

E

L

L

E

I

S

N

L

K

G

G

V

L

L

A

Y

Y

R31 (= R7) mutation R->A,K: Decreased affinity for glutamine and catalytic activity.
EPN 35:37 (≠ SPT 11:13) binding ATP
41:47 (vs. 17:23, 71% identical) binding ATP
D67 (≠ E43) binding L-glutamine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
212 binding L-glutamine
230 C→R: Decreases catalytic activity 1000-fold, but has no effect on affinity for glutamine. Loss of catalytic activity; when associated with I-256.
231 binding ATP
256 Q→I: Loss of catalytic activity; when associated with R-230.
261:262 binding ATP
269:271 binding ATP
317:324 Interaction with tRNA

Query Sequence

>WP_012975077.1 NCBI__GCF_000010725.1:WP_012975077.1
MSDLVTRFAPSPTGHLHLGHAHSALFGWTTARGAAGRFLLRIEDIDPNRCRPEFERDLIE
DLGWLGLDWDGPVRRQSDHFDDYRAALARLESLGVLYPCFCTRKDIAAEIARAAAAPHGP
GPTSSDGPLYPGTCRHRSPQERAERIGDGESWALRLDVEAARRLTGPLRWHDRARGWQQA
TPELLGDVVLARKDTPTSYHLSVTVDDHLQGVTLVTRGEDLFFATHLHRLLQALLGYDPP
DYHHHGLLRNAAGERLAKRDRAQSLRSLREEGRGPAEVRALAGFPG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory