SitesBLAST

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/583 of 5k3sA

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active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), M266 (≠ L257), G286 (= G278), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

active site: 558
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: 569

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
33% identity, 90% coverage: 7:535/589 of query aligns to 100:625/670 of P17597

query
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P17597

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A122 (= A29) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ N31) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (≠ V51) binding thiamine diphosphate
S186 (= S94) binding FAD
P197 (= P105) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ A107) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q115) binding thiamine diphosphate
K220 (= K128) binding (R)-imazaquin
R246 (= R154) binding (R)-imazaquin; binding FAD
K256 (≠ I164) binding chlorimuron-ethyl
G308 (= G212) binding FAD
TL 331:332 (= TL 237:238) binding FAD
C340 (≠ D246) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (≠ VGLQ 255:258) binding FAD
GVRFD 371:375 (≠ GNRWA 278:282) binding FAD
DR 376:377 (≠ NR 283:284) binding chlorimuron-ethyl
DI 395:396 (= DI 302:303) binding FAD
DV 414:415 (≠ DA 321:322) binding FAD
QH 487:488 (≠ LS 395:396) binding thiamine diphosphate
GG 508:509 (≠ GQ 416:417) binding FAD
GAM 511:513 (≠ GPL 419:421) binding thiamine diphosphate
D538 (= D446) binding Mg(2+)
DGS 538:540 (≠ DYD 446:448) binding thiamine diphosphate
N565 (= N473) binding Mg(2+)
NQHLGM 565:570 (≠ NAYLGL 473:478) binding thiamine diphosphate
H567 (≠ Y475) binding Mg(2+)
W574 (≠ A482) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.

Sites not aligning to the query:

653 binding chlorimuron-ethyl; S→A: No effect on catalytic activity or sensitivity to herbicides.; S→F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; S→N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; S→T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 8et4A

query
sites
8et4A

A

G

A

A

E

D

A

I

A

L

V

V

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E

V

A

L

L

E

E

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R

E

Q

G

G

I

V

D

E

V

T

C

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F

F

G

A

V

Y

P

P

G

G

A

G

A

A

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S

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E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V

E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

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G

G

V

I

C

C

I

I

G

A

T

T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F

F

Q

Q

A

E

I

T

N

P

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E

I

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A

T

K

R

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I

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T

K

K

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A

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L

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T

L

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A

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I

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E

A

A

F

F

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F

L

L

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A

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R
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R

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P

G

G

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P

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V

L

L

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L

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D

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I

Q

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M

Q

A

Q

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L

I

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N

D

W

E

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Q

Y

A

E

M

P

R

L

L

P

P

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G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

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A

P

A

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T

D

R

-

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L

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E

K

Q

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I

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A

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L

F

I

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S

A

E

A

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E

K

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K

P

P

L

V

V

L

V

Y

A

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G
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G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

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K

E

L

L

V

G

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R

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F

A

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L

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T

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M

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L

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L

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S

A

L

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M

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L

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L
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Y

Y

G

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N

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S

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D

F

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L

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L

G

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G

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x
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R

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F

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x
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N

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x
V

T

T

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G

S

K

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L

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A

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F

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A

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A

L

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K

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V

A

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-

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R

L

F

K

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L

F

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-

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x
M

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A

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F

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M

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G

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G

L

L

P

P

A

A

L

I

G

G

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A

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A

A

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N

P

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K

D

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M

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x
Q

E

E

L

L

A

A

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G

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A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
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N

A

Q

Y
x
H

L
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L

G
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G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

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R

D

A

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F

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Q

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M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

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Q

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E

I

D

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Y

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G

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D

N

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M

V

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A

L

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A

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A

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A

L

C

G

G

C

I

K

P

A

A

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A

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K

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x
K

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L

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E

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A

F

I

binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: F370 (≠ R361), D453 (= D446), M458 (≠ F451), Q461 (≠ E454), N480 (= N473), H482 (≠ Y475), K533 (≠ P528)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (≠ L257), R292 (= R284), M485 (≠ L478), W489 (≠ A482)

Sites not aligning to the query:

binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: 568

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 5wj1A

query
sites
5wj1A

A

G

A

A

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D

A

I

A

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A

L

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G

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x
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A
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A

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A

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A

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x
E

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Q

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G

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A

A

A

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E

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Y

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A

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-

A

S

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G

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C

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T

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E

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R

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G

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L

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N

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L

L

L

V

L

N

N

N
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x
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L
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L

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|
G

L
x
M

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x
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M

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x
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H

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A

L

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A

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A

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A

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G

C

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A

A

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active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ V255), G286 (= G278), R288 (= R280), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (≠ L257), D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 5k6tA

query
sites
5k6tA

A

G

A

A

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A

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E

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x
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x
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active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (≠ Q258), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), G286 (= G278), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477)

Sites not aligning to the query:

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 5k6rA

query
sites
5k6rA

A

G

A

A

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D

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A

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E

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L

L

L

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L

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|
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x
H

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|
L

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|
G

L
x
M

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x
V

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x
W

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D

A

R

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F

Q

Y

M

K

D

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F

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Q

R

V

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H

L

-

S

T

F

F

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L

N

G

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N

P

A

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Q

E

E

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D

G

E

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I

Y

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A

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C

I

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A

A

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A

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I

active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), M266 (≠ L257), G286 (= G278), R288 (= R280), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), G328 (≠ S320), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), D453 (= D446), G454 (≠ Y447), S455 (≠ D448), M458 (≠ F451), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1z8nA

query
sites
1z8nA

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active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K128), R161 (= R154), Y191 (= Y184), R194 (vs. gap), D291 (≠ N283), R292 (= R284), D312 (≠ E304), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473)
binding thiamine diphosphate: V400 (≠ I393), G401 (= G394), Q402 (≠ L395), H403 (≠ S396), G426 (= G419), M428 (≠ L421), G452 (= G445), G454 (≠ Y447), S455 (≠ D448), N480 (= N473), H482 (≠ Y475), L483 (= L476), G484 (= G477), M485 (≠ L478), V486 (≠ I479)

Sites not aligning to the query:

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1yi1A

query
sites
1yi1A

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A

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active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (≠ N283), R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), M263 (= M254), L264 (≠ V255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1yi0A

query
sites
1yi0A

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

R

E

Q

G

G

I

V

D

E

V

T

C

V

F

F

G

A

V
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
|
T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

I

T

N

P

V

I

P

V

D

E

I

V

A

T

K

R

P

S

V

I

T

T

K

K

W

H

A

N

L

Y

T

L

V

V

L

M

E

D

P

V

A

E

Q

D

V

I

P

P

Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

I
x
K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

A

E

I

F

P

D

N

D

W

E

E

T

Q

Y

A

E

M

P

R

L

L

P

P

V

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

A

P

A

E

T

D

R

-

A

S

Q

H

V

L

E

E

K

Q

A

I

L

V

A

R

M

L

F

I

A

S

A

E

A

S

E

K

R

K

P

P

L

V

V

L

V

Y

A

V

G
|
G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

L

V

G

E

R

F

F

A

V

E

E

L

L

L

T

G

G

V

I

P

P

V

V

I

A

P

S

T
|
T

L
|
L

M
|
M

G

G

W

L

G

G

T

S

I

Y

P

P

D

C

D

D

H

D

P

E

L

L

M

S

A

L

G

H

M

M

V
x
L

G
|
G

L
x
M

Q
x
H

T

-

A

G

H

T

R

V

Y

Y

G

A

N

N

A

Y

T

A

M

V

L

E

K

H

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G
|
G

N
x
V

R
|
R

W

F

A
x
D

N
x
D

R
|
R

H
x
V

T

T

G

G

S

K

V

L

E

E

V

A

Y

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

D
|
D

I
|
I

E

D

P

S

T

A

Q

E

I

I

G

G

R

K

V

N

F

K

M

T

P

P

D

H

L

V

G

S

I

V

V

C

S
x
G

D
|
D

A
x
V

G

K

A

L

A

A

L

L

D

Q

L

G

F

M

I

N

E

K

V

V

A

L

R

-

E

E

W

N

K

R

A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

L

H

S

R

F

R

K

S

T

D

F

F

G

D

E

Q

A

V

I

P

P

M

-

K

-

P

P

Q

Q

R

Y

V

A

Y

I

Q

K

E

V

M

L

N

D

S

E

A

L

F

T

G

D

Q

G

D

K

T

A

T

I

Y

I

V

S

T

T

T

G

I
x
V

G

G

L

Q

S

H

Q

Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

K

K

P

P

R

R

H

Q

W

W

I

L

N

S

C

S

G
|
G

Q
x
G

A

L

G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

D

G

G

D
|
D

Y

G

D

S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
|
N

A

Q

Y
x
H

L
|
L

G

G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

-

V

P

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

T

T

E

K

P

K

D

A

R

D

I

L

Q

R

E

E

A

A

F

I

active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ N283), R292 (= R284), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), R292 (= R284), V293 (≠ H285), D310 (= D302), I311 (= I303), G328 (≠ S320), D329 (= D321), V330 (≠ A322), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1yhzA

query
sites
1yhzA

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

R

E

Q

G

G

I

V

D

E

V

T

C

V

F

F

G

A

V
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
|
T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

I

T

N

P

V

I

P

V

D

E

I

V

A

T

K

R

P

S

V

I

T

T

K

K

W

H

A

N

L

Y

T

L

V

V

L

M

E

D

P

V

A

E

Q

D

V

I

P

P

Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

I
x
K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

A

E

I

F

P

D

N

D

W

E

E

T

Q

Y

A

E

M

P

R

L

L

P

P

V

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

A

P

A

E

T

D

R

-

A

S

Q

H

V

L

E

E

K

Q

A

I

L

V

A

R

M

L

F

I

A

S

A

E

A

S

E

K

R

K

P

P

L

V

V

L

V

Y

A

V

G

G

G
|
G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

L

V

G

E

R

F

F

A

V

E

E

L

L

L

T

G

G

V

I

P

P

V

V

I

A

P

S

T
|
T

L
|
L

M
|
M

G

G

W

L

G

G

T

S

I

Y

P

P

D

C

D

D

H

D

P

E

L

L

M

S

A

L

G

H

M

M

V
x
L

G

G

L
x
M

Q
x
H

T

-

A

G

H

T

R

V

Y

Y

G

A

N

N

A

Y

T

A

M

V

L

E

K

H

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G
|
G

N
x
V

R
|
R

W

F

A
x
D

N
x
D

R
|
R

H
x
V

T

T

G

G

S

K

V

L

E

E

V

A

Y

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

D
|
D

I
|
I

E

D

P

S

T

A

Q

E

I

I

G

G

R

K

V

N

F

K

M

T

P

P

D

H

L

V

G

S

I

V

V

C

S

G

D
|
D

A
x
V

G

K

A

L

A

A

L

L

D

Q

L

G

F

M

I

N

E

K

V

V

A

L

R

-

E

E

W

N

K

R

A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

L

H

S

R

F

R

K

S

T

D

F

F

G

D

E

Q

A

V

I

P

P

M

-

K

-

P

P

Q

Q

R

Y

V

A

Y

I

Q

K

E

V

M

L

N

D

S

E

A

L

F

T

G

D

Q

G

D

K

T

A

T

I

Y

I

V

S

T

T

T

G

I
x
V

G

G

L

Q

S

H

Q
|
Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

K

K

P

P

R

R

H

Q

W

W

I

L

N

S

C

S

G
|
G

Q
x
G

A

L

G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

D

G

G

D
|
D

Y

G

D

S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
|
N

A

Q

Y
x
H

L
|
L

G

G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

-

V

P

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

T

T

E

K

P

K

D

A

R

D

I

L

Q

R

E

E

A

A

F

I

active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1yhyA

query
sites
1yhyA

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

R

E

Q

G

G

I

V

D

E

V

T

C

V

F

F

G

A

V
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
|
T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

I

T

N

P

V

I

P

V

D

E

I

V

A

T

K

R

P

S

V

I

T

T

K

K

W

H

A

N

L

Y

T

L

V

V

L

M

E

D

P

V

A

E

Q

D

V

I

P

P

Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

I
x
K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

A

E

I

F

P

D

N

D

W

E

E

T

Q

Y

A

E

M

P

R

L

L

P

P

V

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

A

P

A

E

T

D

R

-

A

S

Q

H

V

L

E

E

K

Q

A

I

L

V

A

R

M

L

F

I

A

S

A

E

A

S

E

K

R

K

P

P

L

V

V

L

V

Y

A

V

G
|
G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

L

V

G

E

R

F

F

A

V

E

E

L

L

L

T

G

G

V

I

P

P

V

V

I

A

P

S

T
|
T

L
|
L

M
|
M

G

G

W

L

G

G

T

S

I

Y

P

P

D

C

D

D

H

D

P

E

L

L

M

S

A

L

G

H

M

M

V
x
L

G
|
G

L
x
M

Q
x
H

T

-

A

G

H

T

R

V

Y

Y

G

A

N

N

A

Y

T

A

M

V

L

E

K

H

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G
|
G

N
x
V

R
|
R

W

F

A
x
D

N
x
D

R
|
R

H
x
V

T

T

G

G

S

K

V

L

E

E

V

A

Y

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

D
|
D

I
|
I

E

D

P

S

T

A

Q

E

I

I

G

G

R

K

V

N

F

K

M

T

P

P

D

H

L

V

G

S

I

V

V

C

S

G

D
|
D

A
x
V

G

K

A

L

A

A

L

L

D

Q

L

G

F

M

I

N

E

K

V

V

A

L

R

-

E

E

W

N

K

R

A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

L

H

S

R

F

R

K

S

T

D

F

F

G

D

E

Q

A

V

I

P

P

M

-

K

-

P

P

Q

Q

R

Y

V

A

Y

I

Q

K

E

V

M

L

N

D

S

E

A

L

F

T

G

D

Q

G

D

K

T

A

T

I

Y

I

V

S

T

T

T

G

I
x
V

G

G

L

Q

S

H

Q
|
Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

K

K

P

P

R

R

H

Q

W

W

I

L

N

S

C

S

G
|
G

Q
x
G

A

L

G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

D

G

G

D
|
D

Y

G

D

S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
|
N

A

Q

Y
x
H

L
|
L

G

G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

-

V

P

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

T

T

E

K

P

K

D

A

R

D

I

L

Q

R

E

E

A

A

F

I

active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (≠ N283), R292 (= R284), V486 (≠ I479), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G222 (= G211), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), G265 (= G256), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 14:540/582 of 1ybhA

query
sites
1ybhA

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

R

E

Q

G

G

I

V

D

E

V

T

C

V

F

F

G

A

V
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
|
T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

I

T

N

P

V

I

P

V

D

E

I

V

A

T

K

R

P

S

V

I

T

T

K

K

W

H

A

N

L

Y

T

L

V

V

L

M

E

D

P

V

A

E

Q

D

V

I

P

P

Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

I
x
K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

A

E

I

F

P

D

N

D

W

E

E

T

Q

Y

A

E

M

P

R

L

L

P

P

V

G

Y

Y

-

M

-

S

-

R

-

M

-

P

K

K

P

P

A

P

A

E

T

D

R

-

A

S

Q

H

V

L

E

E

K

Q

A

I

L

V

A

R

M

L

F

I

A

S

A

E

A

S

E

K

R

K

P

P

L

V

V

L

V

Y

A

V

G

G

G
|
G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

L

V

G

E

R

F

F

A

V

E

E

L

L

L

T

G

G

V

I

P

P

V

V

I

A

P

S

T
|
T

L
|
L

M
|
M

G

G

W

L

G

G

T

S

I

Y

P

P

D

C

D

D

H

D

P

E

L

L

M

S

A

L

G

H

M

M

V
x
L

G

G

L
x
M

Q
x
H

T

-

A

G

H

T

R

V

Y

Y

G

A

N

N

A

Y

T

A

M

V

L

E

K

H

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G
|
G

N
x
V

R
|
R

W

F

A
x
D

N
x
D

R
|
R

H
x
V

T

T

G

G

S

K

V

L

E

E

V

A

Y

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

D
|
D

I
|
I

E

D

P

S

T

A

Q

E

I

I

G

G

R

K

V

N

F

K

M

T

P

P

D

H

L

V

G

S

I

V

V

C

S

G

D
|
D

A
x
V

G

K

A

L

A

A

L

L

D

Q

L

G

F

M

I

N

E

K

V

V

A

L

R

-

E

E

W

N

K

R

A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

L

H

S

R

F

R

K

S

T

D

F

F

G

D

E

Q

A

V

I

P

P

M

-

K

-

P

P

Q

Q

R

Y

V

A

Y

I

Q

K

E

V

M

L

N

D

S

E

A

L

F

T

G

D

Q

G

D

K

T

A

T

I

Y

I

V

S

T

T

T

G

I
x
V

G

G

L

Q

S

H

Q
|
Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

K

K

P

P

R

R

H

Q

W

W

I

L

N

S

C

S

G
|
G

Q
x
G

A

L

G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

D

G

G

D
|
D

Y

G

D

S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
|
N

A

Q

Y
x
H

L
|
L

G

G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

-

V

P

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

T

T

E

K

P

K

D

A

R

D

I

L

Q

R

E

E

A

A

F

I

active site: Y33 (≠ V25), G35 (= G27), G36 (≠ A28), A37 (= A29), S38 (≠ I30), E59 (≠ V51), T82 (= T75), F121 (= F114), Q122 (= Q115), E123 (≠ A116), K171 (≠ I164), M266 (≠ L257), V293 (≠ H285), V400 (≠ I393), G426 (= G419), M428 (≠ L421), D453 (= D446), N480 (= N473), H482 (≠ Y475), L483 (= L476), M485 (≠ L478), V486 (≠ I479), W489 (≠ A482)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (≠ L257), D291 (≠ N283), R292 (= R284), M485 (≠ L478), W489 (≠ A482)
binding flavin-adenine dinucleotide: R161 (= R154), G223 (= G212), G224 (= G213), T246 (= T237), L247 (= L238), M248 (= M239), L264 (≠ V255), M266 (≠ L257), H267 (≠ Q258), G286 (= G278), V287 (≠ N279), R288 (= R280), D290 (≠ A282), V293 (≠ H285), D310 (= D302), I311 (= I303), D329 (= D321), V330 (≠ A322), Q404 (= Q397), M405 (≠ I398), G423 (= G416), G424 (≠ Q417)
binding magnesium ion: D453 (= D446), N480 (= N473), H482 (≠ Y475)

Sites not aligning to the query:

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 13:539/582 of 3ea4A

query
sites
3ea4A

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

R

E

Q

G

G

I

V

D

E

V

T

C

V

F

F

G

A

V
x
Y

P

P

G
|
G

A
x
G

A
|
A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

L

L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
|
T

S

S

G

G

P

P

A

G

G

A

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N

M

L

I

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G

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Y

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A

A

I

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A

L

D

D

S

S

I

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P

I

L

L

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C

A

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I

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T

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G

Q

Q

A

V

P

P

R

R

A

R

R

M

L

I

H

G

K

T

E

D

D

A

F
|
F

Q
|
Q

A
x
E

I

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N

P

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I

P

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D

E

I

V

A

T

K

R

P

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A

N

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Y

T

L

V

V

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M

E

D

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A

E

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D

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I

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Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

R

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

L

L

I

V

D

D

L

V

P

P

I
x
K

D

D

V

I

Q

Q

M

Q

A

Q

E

L

I

A

E

I

F

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D

N

D

W

E

E

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Q

Y

A

E

M

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R

L

L

P

P

V

G

Y

Y

-

M

-

S

-

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-

M

-

P

K

K

P

P

A

P

A

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T

D

R

-

A

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A

I

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A

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F

I

A

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A

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A

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K

R

K

P

P

L

V

V

L

V

Y

A

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G

I

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I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

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R

F

F

A

V

E

E

L

L

L

T

G

G

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I

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P

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I

A

P

T

T
|
T

L
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L

M
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M

G

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L

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I

Y

P

P

D

C

D

D

H

D

P

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L

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x
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G

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x
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x
H

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-

A

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Y

Y

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A

N

N

A

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A

M

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S

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D

F

L

V

L

L

L

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G

N

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R

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x
D

N
x
D

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R

H
x
V

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D

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I

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I

I

G

G

R

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V

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C

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x
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D

A
x
V

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A

L

A

A

L

L

D

Q

L

G

F

M

I

N

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K

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E

E

W

N

K

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A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

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H

S

R

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R

K

S

T

D

F

F

G

D

E

Q

A

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I

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-

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-

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P

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Q

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Y

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A

Y

I

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K

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M

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N

D

S

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A

L

F

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G

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Q

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D

K

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A

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I

Y

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G

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x
V

G
|
G

L
x
Q

S
x
H

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Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

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P

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R

R

H

Q

W

W

I

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N

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C

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G

Q

G

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G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

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G
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G

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D

Y
x
G

D
x
S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
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N

A

Q

Y
x
H

L
|
L

G
|
G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

P

V

-

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

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T

E

K

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K

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A

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E

A

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F

I

active site: Y32 (≠ V25), G34 (= G27), G35 (≠ A28), A36 (= A29), S37 (≠ I30), E58 (≠ V51), T81 (= T75), F120 (= F114), Q121 (= Q115), E122 (≠ A116), K170 (≠ I164), M265 (≠ L257), V292 (≠ H285), V399 (≠ I393), G425 (= G419), M427 (≠ L421), D452 (= D446), N479 (= N473), H481 (≠ Y475), L482 (= L476), M484 (≠ L478), V485 (≠ I479), W488 (≠ A482)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (≠ N283), R291 (= R284), W488 (≠ A482)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G211), G222 (= G212), G223 (= G213), T245 (= T237), L246 (= L238), M247 (= M239), L263 (≠ V255), G264 (= G256), M265 (≠ L257), H266 (≠ Q258), G285 (= G278), R287 (= R280), D289 (≠ A282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (≠ S320), D328 (= D321), V329 (≠ A322), M404 (≠ I398), G422 (= G416)
binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ Y475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ I393), G400 (= G394), Q401 (≠ L395), H402 (≠ S396), M427 (≠ L421), G451 (= G445), D452 (= D446), G453 (≠ Y447), S454 (≠ D448), N479 (= N473), H481 (≠ Y475), L482 (= L476), G483 (= G477), M484 (≠ L478), V485 (≠ I479)

Sites not aligning to the query:

active site: 557
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: 567

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
33% identity, 90% coverage: 6:535/589 of query aligns to 13:539/582 of 3e9yA

query
sites
3e9yA

A

G

A

A

E

D

A

I

A

L

V

V

R

E

V

A

L

L

E

E

R

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E

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E

V

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C

V

F

F

G

A

V
x
Y

P

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G
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G

A
x
G

A
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A

I
x
S

N

M

P

E

F

I

Y

H

A

Q

A

A

M

L

Q

T

R

R

R

S

G

S

T

S

M

I

R

R

H

N

V

V

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L

A

P

R

R

H

H

V
x
E

E

Q

G

G

A

G

S

V

H

F

M

A

A

A

E

E

G

G

Y

Y

T

A

R

R

A

S

E

-

A

S

G

G

N

K

I

P

G

G

V

I

C

C

I

I

G

A

T
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T

S

S

G

G

P

P

A

G

G

A

T

T

D

N

M

L

I

V

T

S

G

G

L

L

Y

A

S

D

A

A

I

L

A

L

D

D

S

S

I

V

P

P

I

L

L

V

C

A

I

I

T

T

G

G

Q

Q

A

V

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P

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A

R

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M

L

I

H

G

K

T

E

D

D

A

F
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F

Q
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Q

A
x
E

I

T

N

P

V

I

P

V

D

E

I

V

A

T

K

R

P

S

V

I

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T

K

K

W

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A

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T

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M

E

D

P

V

A

E

Q

D

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Y

R

V

I

F

I

Q

E

A

A

F

F

H

F

L

L

M

A

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S

G

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R

P

P

G

G

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P

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V

L

L

I

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D

D

L

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x
K

D

D

V

I

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Q

M

Q

A

Q

E

L

I

A

E

I

F

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D

N

D

W

E

E

T

Q

Y

A

E

M

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R

L

L

P

P

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G

Y

Y

-

M

-

S

-

R

-

M

-

P

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K

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P

A

P

A

E

T

D

R

-

A

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Q

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V

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E

E

K

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A

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A

R

M

L

F

I

A

S

A

E

A

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E

K

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K

P

P

L

V

V

L

V

Y

A

V

G
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G

I

C

I

L

N

N

A

-

D

-

A

S

S

S

D

D

K

E

L

L

V

G

E

R

F

F

A

V

E

E

L

L

L

T

G

G

V

I

P

P

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V

I

A

P

T

T
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T

L
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L

M
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M

G

G

W

L

G

G

T

S

I

Y

P

P

D

C

D

D

H

D

P

E

L

L

M

S

A

L

G

H

M

M

V
x
L

G

G

L
x
M

Q

H

T

-

A

G

H

T

R

V

Y

Y

G

A

N

N

A

Y

T

A

M

V

L

E

K

H

S

S

D

D

F

L

V

L

L

L

G

A

I

F

G
|
G

N

V

R
|
R

W

F

A
x
D

N
x
D

R
|
R

H
x
V

T

T

G

G

S

K

V

L

E

E

V

A

Y

F

T

A

K

S

G

R

R

A

K

K

F

I

V

V

H

H

V

I

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D

I
|
I

E

D

P

S

T

A

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I

I

G

G

R

K

V

N

F

K

M

T

P

P

D

H

L

V

G

S

I

V

V

C

S
x
G

D
|
D

A
x
V

G

K

A

L

A

A

L

L

D

Q

L

G

F

M

I

N

E

K

V

V

A

L

R

-

E

E

W

N

K

R

A

A

E

E

G

E

R

L

F

K

K

L

D

D

L

F

G

G

G

V

W

W

V

R

K

N

-

E

-

L

D

N

C

V

Q

Q

G

K

R

Q

K

K

R

F

S

P

M

L

H

S

R

F

R

K

S

T

D

F

F

G

D

E

Q

A

V

I

P

P

M

-

K

-

P

P

Q

Q

R

Y

V

A

Y

I

Q

K

E

V

M

L

N

D

S

E

A

L

F

T

G

D

Q

G

D

K

T

A

T

I

Y

I

V

S

T

T

T

G

I
x
V

G
|
G

L
x
Q

S
x
H

Q

Q

I
x
M

A

W

G

A

A

A

Q

Q

F

F

L

Y

H

N

V

Y

Y

K

K

K

P

P

R

R

H

Q

W

W

I

L

N

S

C

S

G
|
G

Q

G

A

L

G
|
G

P

A

L
x
M

G

G

W

F

T

G

L

L

P

P

A

A

L

I

G

G

V

A

R

S

V

V

A

A

D

N

P

P

K

D

R

A

R

I

I

V

V

V

A

D

L

I

S

D

G
|
G

D
|
D

Y
x
G

D
x
S

F

F

Q

I

F

M

M

N

I

V

E

Q

E

E

L

L

A

A

V

T

G

I

A

R

Q

V

H

E

K

N

L

L

P

P

Y

V

I

K

H

V

V

L

L

L

V

L

N

N

N
|
N

A

Q

Y
x
H

L
|
L

G
|
G

L
x
M

I
x
V

R

M

Q

Q

A
x
W

Q

E

R

D

A

R

F

F

Q

Y

M

K

D

A

F

N

Q

R

V

A

Q

H

L

-

S

T

F

F

E

L

N

G

I

D

N

P

A

A

P

Q

E

E

I

D

G

E

V

I

Y

F

G

P

V

-

D

N

H

M

V

L

A

L

V

F

A

A

E

A

G

A

L

C

G

G

C

I

K

P

A

A

I

A

R

R

V

V

T

T

E

K

P

K

D

A

R

D

I

L

Q

R

E

E

A

A

F

I

active site: Y32 (≠ V25), G34 (= G27), G35 (≠ A28), A36 (= A29), S37 (≠ I30), E58 (≠ V51), T81 (= T75), F120 (= F114), Q121 (= Q115), E122 (≠ A116), K170 (≠ I164), M265 (≠ L257), V292 (≠ H285), V399 (≠ I393), G425 (= G419), M427 (≠ L421), D452 (= D446), N479 (= N473), H481 (≠ Y475), L482 (= L476), M484 (≠ L478), V485 (≠ I479), W488 (≠ A482)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (≠ N283), R291 (= R284), W488 (≠ A482)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R154), G221 (= G211), G222 (= G212), G223 (= G213), T245 (= T237), L246 (= L238), M247 (= M239), L263 (≠ V255), G285 (= G278), R287 (= R280), D289 (≠ A282), R291 (= R284), D309 (= D302), I310 (= I303), G327 (≠ S320), D328 (= D321), V329 (≠ A322), M404 (≠ I398), G422 (= G416)
binding magnesium ion: D452 (= D446), N479 (= N473), H481 (≠ Y475)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (≠ I393), G400 (= G394), Q401 (≠ L395), H402 (≠ S396), M427 (≠ L421), G451 (= G445), G453 (≠ Y447), S454 (≠ D448), N479 (= N473), H481 (≠ Y475), L482 (= L476), G483 (= G477), M484 (≠ L478), V485 (≠ I479)

Sites not aligning to the query:

active site: 557
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: 567

Query Sequence

>WP_012975773.1 NCBI__GCF_000010725.1:WP_012975773.1
MARMTAAEAAVRVLEREGIDVCFGVPGAAINPFYAAMQRRGTMRHVLARHVEGASHMAEG
YTRAEAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLHKEDFQAINVP
DIAKPVTKWALTVLEPAQVPYVFQQAFHLMRSGRPGPVLIDLPIDVQMAEIEFDDETYEP
LPVYKPAATRAQVEKALAMFAAAERPLVVAGGGIINADASDKLVEFAELLGVPVIPTLMG
WGTIPDDHPLMAGMVGLQTAHRYGNATMLKSDFVLGIGNRWANRHTGSVEVYTKGRKFVH
VDIEPTQIGRVFMPDLGIVSDAGAALDLFIEVAREWKAEGRFKDLGGWVKDCQGRKRSMH
RRSDFDQVPMKPQRVYQEMNSAFGQDTTYVTTIGLSQIAGAQFLHVYKPRHWINCGQAGP
LGWTLPAALGVRVADPKRRIVALSGDYDFQFMIEELAVGAQHKLPYIHVLVNNAYLGLIR
QAQRAFQMDFQVQLSFENINAPEIGVYGVDHVAVAEGLGCKAIRVTEPDRIQEAFTQAQA
WMDQYQVPVVVELVLERVTNISMGTDIDNVTEFEETLDLPVEESETVLV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory