SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H30) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D34) mutation D->N,E: Little effect on the kinetic properties.
H81 (= H81) mutation to A: 5-fold decrease in glutamine affinity.
A105 (≠ H106) mutation to H: Little effect on the kinetic properties.
E349 (= E368) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
33% identity, 56% coverage: 1:380/684 of query aligns to 1:368/557 of P78753

query
sites
P78753

M

M

C

C

G

G

V

I

A

L

G

A

V

V

L

H

A

H

G

V

S

A

R

E

A

D

E

I

P

E

A

A

G

F

L

K

D

P

E

K

L

A

K

L

R

H

M

L

I

S

A

K

M

Q

L

L

S

R

H

H

R

R

G

G

P

P

D

D

G

W

Y

S

G

G

F

K

Y

A

R

I

D

R

D

N

R

Q

I

T

G

I

L

L

A

C

H

H

A

E

R

R

L

L

S

A

I

I

V

V

G

G

L

V

A

E

G

S

G

G

F

A

Q

Q

P

P

I

L

H

V

N

S

E

D

D

D

R

G

S

K

L

L

W

V

I

L

S

T

F

V

N

N

G

G

E

E

I

I

F

Y

N

N

H

H

V

L

E

K

L

L

R

R

R

E

D

N

L

L

E

K

A

G

R

-

G

N

H

Y

R

K

F

F

Y

K

T

T

R

Y

T

S

D

D

T

C

E

E

V

V

I

I

V

L

H

Y

A

L

F

Y

E

R

E

E

Y

H

G

G

P

P

A

A

T

C

W

A

A

N

R

M

L

L

N

D

G

G

Q

M

F

F

A

S

I

W

C

V

L

L

W

Y

D

D

A

Q

T

D

R

K

R

D

E

K

L

V

W

V

L

A

V

A

R

R

D

D

R

P

L

I

G

G

I

I

L

T

P

T

L

L

-

Y

-

Q

-

G

F

F

Y

S

A

S

R

D

R

S

G

P

D

D

H

T

L

A

V

Y

F

F

A

A

S

S

E

E

A

L

K

K

A

A

L

L

F

-

G

-

R

-

V

-

A

-

P

-

A

-

F

-

D

-

H

H

A

P

R

V

L

C

A

D

Q

K

V

I

F

I

T

-

H

-

W

-

S

-

A

A

A

F

P

P

H

P

G

G

S

H

V

Y

F

Y

E

-

R

-

V

-

E

-

S

-

V

-

P

-

A

-

T

-

A

-

I

-

R

-

V

-

D

D

S

S

T

E

L

T

A

K

L

Q

H

T

V

V

D

-

R

R

Y

Y

W

F

Q

K

P

P

D

S

F

W

T

W

A

D

D

E

P

N

A

K

L

I

A

P

K

S

L

N

S

P

L

V

D

D

E

Y

A

K

A

L

D

-

R

-

L

L

E

R

E

E

K

T

L

L

L

E

D

A

A

S

I

V

R

R

L

K

R

R

L

L

R

M

A

A

D

E

V

V

P

P

V

Y

G

G

V

V

Y

L

I

L

S

S

G

G

L

L

D

D

S

S

V

L

I

I

A

A

A

S

L

I

A

A

-

A

-

R

-

E

-

T

R

E

K

K

L

L

-

A

D

N

T

S

T

T

S

S

-

Q

-

S

-

E

-

A

-

R

-

T

-

I

-

T

-

A

-

W

-

P

-

K

M

L

H

H

S

S

F

F

G

A

V

I

R

G

F

L

T

-

D

-

A

P

G

G

F

S

D

P

E

D

T

L

P

L

Q

A

R

R

L

K

V

V

A

A

G

D

H

F

V

L

G

H

T

T

G

F

H

H

D

E

I

H

L

T

C

F

G

T

P

I

R

D

D

E

I

G

Q

L

A

D

A

A

L

L

P

R

D

D

V

V

V

I

R

Y

H

H

S

L

E

E

T

T

P

Y

L

D

V

V

R

T

T

T

-

I

-

R

A

A

P

S

A

T

P

P

L

M

F

Y

L

L

S

S

R

R

L

K

V

I

R

K

E

A

S

Q

G

G

I

V

R

K

V

M

V

V

L

L

S

S

G

G

E

E

G

G

A

S

D

D

E

E

W

I

L

F

A

G

G

G

Y

Y

D

L

L

Y

F

F

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
29% identity, 56% coverage: 1:380/684 of query aligns to 1:377/561 of P08243

query
sites
P08243

M

M

C
|
C

G

G

V

I

A

W

G
x
A

V

L

L

F

A

G

G

S

S

D

R

D

A

C

E

L

P

-

A

-

G

-

L

-

D

-

E

S

L

V

K

Q

R

C

M

L

I

S

A

A

M

M

-

K

L

I

S

A

H

H

R

R

G

G

P

P

D

D

G

A

Y

F

G

R

F

F

-

E

-

N

-

V

-

N

-

G

Y

Y

R

T

D

N

D

C

R

C

I

F

G

G

L

F

A

H

H

-

A

-

R

R

L

L

S

A

I

V

V

V

G

D

L

P

A

L

G

F

G

G

F

M

Q

Q

P

P

I

I

H

R

N

V

E

K

D

K

R

Y

-

P

S

Y

L

L

W

W

I

L

S

C

F

Y

N

N

G

G

E

E

I

I

F

Y

N

N

H

H

V

K

E

K

L

M

R

Q

D

H

L

F

E

E

A

-

R

-

G

-

H

F

R

E

F

Y

Y

Q

T

T

R

K

T

V

D

D

T

G

E

E

V

I

I

I

V

L

H

H

A

L

F

Y

E

D

E

K

Y

G

G

G

-

I

P

E

A

Q

T

T

W

I

A

C

R

M

L

L

N

D

G

G

Q

V

F

F

A

A

I

F

C

V

L

L

W

L

D

D

A

T

T

A

R

N

R

K

E

K

L

V

W

F

L

L

V

G

R

R

D

D

R

T

L

Y

G

G

I

V

L

R

P

P

L

L

F

F

Y

K

A

A

R

M

R

T

G

E

D

D

-

G

H

F

L

L

V

A

F

V

A

C

S

S

E

E

A

A

K

K

A

G

L

L

F

V

G

T

G

L

G

K

R

H

V

S

A

A

P

T

A

P

F

F

D

L

H

K

A

V

R

E

-

P

-

F

L

L

A

P

Q

G

V

H

F

Y

T

E

H

V

W

L

S

D

A

L

A

K

P

P

H

N

G

G

S

K

V

V

F

-

E

A

R

S

V

V

E

E

S

M

V

V

P

K

A

Y

A

H

T

H

A

C

I

R

R

D

V
|
V

D

P

-

L

S

H

T

A

L

L

A

Y

L

D

H

N

V

V

D

E

R

K

Y

L

W

F

Q

-

P

P

D

G

F

F

T

-

A

-

D

-

P

-

A

-

L

-

A

-

K

-

L

-

S

E

L

I

D

E

E

T

A

V

A

K

D

N

R

N

L

L

E

R

E

I

K

L

L

F

L

N

D

N

A

A

I

V

R

K

L

K

R

R

L

L

R

M

A

T

D

D

V

R

P

R

V

I

G

G

V

C

Y

L

I

L

S

S

G

G

L

L

D

D

S

S

V

L

I

V

A

A

L

T

A

L

R

L

K

K

L

Q

D

L

T

K

T

E

S

A

M

Q

H

V

S

Q

F

Y

G

P

V

L

R

Q

F

T

T

F

D

A

A

I

G

G

F

M

D

E

E

D

T

S

P

P

Q

D

-

L

-

A

Q

A

R

R

L

K

V

V

A

A

G

D

H

H

V

I

G

G

T

S

G

E

H

H

H

Y

D

E

I

V

L

L

C

F

G

N

P

S

R

E

D

E

I

G

Q

I

A

Q

A

A

L

L

P

D

D

E

V

V

V

I

R

F

H

S

S

L

E

E

T

T

P

Y

L

D

V

I

R

T

T

T

-

V

-

R

A

A

P

S

A

V

P

G

L

M

F

Y

L

L

L

I

S

S

R

K

L

Y

V

I

R

R

E

K

S

N

G

T

I

D

R

S

V

V

L

I

-
x
F

S

S

G

G

E

E

G

G

A

S

D

D

E

E

W

L

L

T

A

Q

G

G

Y

Y

D

I

L

Y

F

F

C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (= V210) to E: in dbSNP:rs1049674
F362 (vs. gap) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
29% identity, 52% coverage: 28:380/684 of query aligns to 23:364/509 of 6gq3A

query
sites
6gq3A

L

I

S

A

H

H

R

R

G

G

P

P

D

D

G

A

Y

F

G

R

F

F

-

E

-

N

-

V

-

N

-

G

Y

Y

R

T

D

N

D

C

R

C

I

F

G

G

L

F

A

H

H

-

A

-

R
|
R

L
|
L

S

A

I
x
V

V
|
V

G

D

L

P

A

L

G

F

G

G

F

M

Q

Q

P

P

I

I

H

R

N

V

E

K

D

K

R

Y

-

P

S

Y

L

L

W

W

I

L

S

C

F
x
Y

N
|
N

G
|
G

E
|
E

I

I

F

Y

N

N

H

H

V

K

E

K

L

M

R

Q

D

H

L

F

E

E

A

-

R

-

G

-

H

F

R

E

F

Y

Y

Q

T

T

R

K

T
x
V

D
|
D

T

G

E

E

V

I

I

I

V

L

H

H

A

L

F

Y

E

D

E

K

Y

G

G

G

-

I

P

E

A

Q

T

T

W

I

A

C

R

M

L

L

N

D

G

G

Q

V

F

F

A

A

I

F

C

V

L

L

W

L

D

D

A

T

T

A

R

N

R

K

E

K

L

V

W

F

L

L

V

G

R

R

D

D

R

T

L

Y

G

G

I

V

L

R

P

P

L

L

F

F

Y

K

A

A

R

M

R

T

G

E

D

D

-

G

H

F

L

L

V

A

F

V

A

C

S

S

E

E

A

A

K

K

A

G

L

L

F

-

G

-

R

-

V

-

A

-

P

-

A

-

F

-

D

-

H

-

A

-

R

-

L

-

A

-

Q

-

V

V

F

T

T

L

H

K

W

H

S

S

A

A

A

T

P

P

H

F

G

L

S

K

V

V

-

E

-

P

-

F

-

L

-

P

-

G

-

H

F

Y

E

E

R

V

V

L

E

D

S

L

V

K

P

P

A

N

A

G

T

K

A

V

I

A

R

S

V

V

D

E

S

-

T

M

L

V

A

K

L

Y

H

H

V

H

D

C

R

R

Y

D

W

V

Q

F

P

P

D

G

F

F

T

-

A

-

D

-

P

-

A

-

L

-

A

-

K

-

L

-

S

E

L

I

D

E

E

T

A

V

A

K

D

N

R

N

L

L

E

R

E

I

K

L

L

F

L

N

D

N

A

A

I

V

R

K

L

K

R

R

L

L

R

M

A

T

D

D

V

R

P

R

V

I

G

G

V

C

Y

L

I

L

S

S

G

G

L

L

D

D

S

S

V

L

I

V

A

A

L

T

A

L

R

L

K

K

L

Q

D

L

T

K

T

E

S

A

M

Q

H

V

S

Q

F

Y

G

P

V

L

R

Q

F

T

T

F

D

A

A

I

G

G

F

M

D

E

E

D

T

S

P

P

Q

D

-

L

-

A

Q

A

R

R

L

K

V

V

A

A

G

D

H

H

V

I

G

G

T

S

G

E

H

H

H

Y

D

E

I

V

L

L

C

F

G

N

P

S

R

E

D

E

I

G

Q

I

A

Q

A

A

L

L

P

D

D

E

V

V

V

I

R

F

H

S

S

L

E

E

T

T

P

Y

L

D

V

I

R
x
T

T

T

-

V

-
x
R

A

A

P

S

A

V

P

G

L

M

F

Y

L

L

L

I

S

S

R

K

L

Y

V

I

R

R

E

K

S

N

G

T

I

D

R

S

V

V

L

I

-

F

S

S

G

G

E

E

G

G

A

S

D

D

E

E

W

L

L

T

A

Q

G

G

Y

Y

D

I

L

Y

F

F

active site: L49 (= L51), N74 (= N75), G75 (= G76), T324 (≠ R343), R327 (vs. gap)
binding 5-oxo-l-norleucine: R48 (= R50), V51 (≠ I53), V52 (= V54), Y73 (≠ F74), N74 (= N75), G75 (= G76), E76 (= E77), V95 (≠ T99), D96 (= D100)

Sites not aligning to the query:

active site: 4
binding 5-oxo-l-norleucine: 1

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
30% identity, 46% coverage: 74:385/684 of query aligns to 64:347/491 of 1mc1A

query
sites
1mc1A

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

H

R

V

D

E

E

L

L

R

L

R

S

D

V

L

L

E

P

A

A

R

G

G

P

H

-

R

-

F

-

Y

A

T

P

R

E

T

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

F

L

E

E

E

R

Y

Y

G

D

P

L

A

H

T

A

W

F

A

R

R

L

L

V

N

N

G

G

Q

R

F

F

A

A

I

T

C

V

L

V

W

R

D

T

A

G

T

D

R

R

R

-

E

-

L

V

W

L

L

L

V

A

R

T

D

D

R

H

L

A

G

G

I

S

L

V

P

P

L

L

F

Y

Y

T

A

C

R

V

R

A

G

P

D

G

H

E

L

V

V

R

F

A

A

S

S

T

E

E

A

A

K

K

A

A

L

L

F

A

G

A

G

G

G

F

R

P

V

L

A

A

P

D

A

A

F

R

D

R

H

V

A

A

R

G

L

L

A

T

Q

G

V

V

F

Y

T

-

H

-

W

-

S

-

A

-

P

-

H

-

G

-

S

-

V

-

F

-

E

-

R

-

V

-

E

-

S

Q

V

V

P

P

A

A

A

G

T

A

A

V

I

M

R

D

V

I

D

D

-

L

-

G

-

S

S

G

T

T

L

A

A

V

L

T

H

H

V

-

D

-

R

R

Y

T

W

W

Q

T

P

P

D

G

F

L

T

S

A

R

D

R

P

-

A

-

L

-

A

-

K

I

L

L

S

P

L

E

D

G

E

E

A

A

A

V

D

A

R

A

L

V

E

R

E

A

K

A

L

L

L

E

D

K

A

A

I

V

R

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

Y
x
V

I

L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

A

A

L

C

A

A

R

H

K

R

-

A

-

G

-

E

L

L

D

D

T

T

T

V

S
|
S

M
|
M

H

G

S

-

F

-

G

-

V

-

R

-

F

-

T

T

D

D

A

T

G

S

F

-

D

N

E

E

T

F

P

R

Q

E

Q

A

R

R

L

A

V

V

A

V

G

D

H

H

V

L

G

R

T

T

G

R

H

H

H

R

D

E

I

I

L

T

C

I

G

P

P

T

R

T

D

E

I

L

Q

L

A

A

A

Q

L

L

P

P

D

Y

V

A

V

V

R

W

H

A

S

S

E

E

T

S

-

V

-

D

P

P

L
x
D

V

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L

L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

S

G

G

P

I

E

R

R

V

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

W

P

L

L

A

G

G

G

Y

-

D

-

L

M

F

H

K

R

E

E

D

D

K

R

V

L

active site: A65 (≠ N75), G66 (= G76), D306 (≠ L341), Y332 (≠ E368)
binding adenosine monophosphate: V231 (≠ Y265), S233 (= S267), S238 (= S272), S256 (= S286), M257 (= M287), G331 (= G367)
binding magnesium ion: D237 (= D271), D335 (= D371)
binding deoxyguanidinoproclavaminic acid: Y310 (≠ A345), Y332 (≠ E368), G333 (= G369), I336 (≠ E372)
binding pyrophosphate 2-: S233 (= S267), G235 (= G269), D237 (= D271), S238 (= S272), D335 (= D371)

Sites not aligning to the query:

active site: 366, 427
binding adenosine monophosphate: 427, 430
binding deoxyguanidinoproclavaminic acid: 357, 366, 427
binding pyrophosphate 2-: 407, 427, 428

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
30% identity, 46% coverage: 74:385/684 of query aligns to 68:352/496 of 1mbzA

query
sites
1mbzA

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

H

R

V

D

E

E

L

L

R

L

R

S

D

V

L

L

E

P

A

A

R

G

G

P

H

-

R

-

F

-

Y

A

T

P

R

E

T

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

F

L

E

E

E

R

Y

Y

G

D

P

L

A

H

T

A

W

F

A

R

R

L

L

V

N

N

G

G

Q

R

F

F

A

A

I

T

C

V

L

V

W

R

D

T

A

G

T

D

R

R

R

-

E

-

L

V

W

L

L

L

V

A

R

T

D

D

R

H

L

A

G

G

I

S

L

V

P

P

L

L

F

Y

Y

T

A

C

R

V

R

A

G

P

D

G

H

E

L

V

V

R

F

A

A

S

S

T

E

E

A

A

K

K

A

A

L

L

F

A

G

A

G

H

G

G

R

-

V

-

A

F

P

P

A

L

F

A

D

D

H

A

A

R

R

R

L

V

A

A

Q

G

V

-

F

-

T

-

H

-

W

-

S

-

A

-

P

-

H

-

G

-

S

-

V

-

F

L

E

T

R

G

V

V

E

Y

S

Q

V

V

P

P

A

A

A

G

T

A

A

V

I

M

R

D

V

I

D

D

-

L

-

G

-

S

S

G

T

T

L

A

A

V

L

T

H

H

V

-

D

-

R

R

Y

T

W

W

Q

T

P

P

D

G

F

L

T

S

A

R

D

R

P

-

A

-

L

-

A

-

K

I

L

L

S

P

L

E

D

G

E

E

A

A

A

V

D

A

R

A

L

V

E

R

E

A

K

A

L

L

L

E

D

K

A

A

I

V

R

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

Y
x
V

I
x
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

A

A

L

C

A

A

R

H

K

R

-

A

-

G

-

E

L

L

D

D

T

T

T

V

S
|
S

M
|
M

H

G

S

-

F

-

G

-

V

-

R

-

F

-

T

T

D

D

A

T

G

S

F

-

D

N

E

E

T

F

P

R

Q

E

Q

A

R

R

L

A

V

V

A

V

G

D

H

H

V

L

G

R

T

T

G

R

H

H

H

R

D

E

I

I

L

T

C

I

G

P

P

T

R

T

D

E

I

L

Q

L

A

A

A

Q

L

L

P

P

D

Y

V

A

V

V

R

W

H

A

S

S

E

E

T

S

-

V

-

D

P

P

L
x
D

V

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

S

G

G

P

I

E

R

R

V

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

W

P

L

L

A

G

G

G

Y

-

D

-

L

M

F

H

K

R

E

E

D

D

K

R

V

L

active site: A69 (≠ N75), G70 (= G76), D311 (≠ L341), Y337 (≠ E368)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ Y265), L237 (≠ I266), S238 (= S267), S243 (= S272), S261 (= S286), M262 (= M287), Y315 (≠ A345), L319 (= L349), G336 (= G367), Y337 (≠ E368), G338 (= G369), D340 (= D371), I341 (≠ E372)
binding magnesium ion: D242 (= D271), D340 (= D371)
binding pyrophosphate 2-: S238 (= S267), G240 (= G269), D242 (= D271), S243 (= S272), D340 (= D371)

Sites not aligning to the query:

active site: 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 362, 371, 432, 434, 435
binding pyrophosphate 2-: 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
29% identity, 46% coverage: 74:385/684 of query aligns to 69:351/485 of 1mb9A

query
sites
1mb9A

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

H

R

V

D

E

E

L

L

R

L

R

S

D

V

L

L

E

P

A

A

R

G

G

P

H

-

R

-

F

-

Y

A

T

P

R

E

T

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

F

L

E

E

E

R

Y

Y

G

D

P

L

A

H

T

A

W

F

A

R

R

L

L

V

N

N

G

G

Q

R

F

F

A

A

I

T

C

V

L

V

W

R

D

T

A

G

T

D

R

R

R

-

E

-

L

V

W

L

L

L

V

A

R

T

D

D

R

H

L

A

G

G

I

S

L

V

P

P

L

L

F

Y

Y

T

A

C

R

V

R

A

G

P

D

G

H

E

L

V

V

R

F

A

A

S

S

T

E

E

A

A

K

K

A

A

L

L

F

A

G

A

G

-

R

-

V

-

A

F

P

P

A

L

F

A

D

D

H

A

A

R

R

R

L

V

A

A

Q

G

V

-

F

-

T

-

H

-

W

-

S

-

A

-

P

-

H

-

G

-

S

-

V

-

F

L

E

T

R

G

V

V

E

Y

S

Q

V

V

P

P

A

A

A

G

T

A

A

V

I

M

R

D

V

I

D

D

-

L

-

G

-

S

S

G

T

T

L

A

A

V

L

T

H

H

V

-

D

-

R

R

Y

T

W

W

Q

T

P

P

D

G

F

L

T

S

A

R

D

R

P

-

A

-

L

-

A

-

K

I

L

L

S

P

L

E

D

G

E

E

A

A

A

V

D

A

R

A

L

V

E

R

E

A

K

A

L

L

L

E

D

K

A

A

I

V

R

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

Y
x
V

I
x
L

S
|
S

G

G

G
|
G

L

I

D
|
D

S
|
S

S

S

V

G

I

V

A

A

L

C

A

A

R

H

K

R

-

A

-

G

-

E

L

L

D

D

T

T

T

V

S
|
S

M
|
M

H

G

S

-

F

-

G

-

V

-

R

-

F

-

T

T

D

D

A

T

G

S

F

-

D

N

E

E

T

F

P

R

Q

E

Q

A

R

R

L

A

V

V

A

V

G

D

H

H

V

L

G

R

T

T

G

R

H

H

H

R

D

E

I

I

L

T

C

I

G

P

P

T

R

T

D

E

I

L

Q

L

A

A

A

Q

L

L

P

P

D

Y

V

A

V

V

R

W

H

A

S

S

E

E

T

S

-

V

-

D

P

P

L
x
D

V

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

S

G

G

P

I

E

R

R

V

R

V

I

L

L

S

T

G
|
G

E
x
Y

G

G

A

A

D
|
D

E

I

W

P

L

L

A

G

G

G

Y

-

D

-

L

M

F

H

K

R

E

E

D

D

K

R

V

L

active site: A70 (≠ N75), G71 (= G76), D310 (≠ L341), Y336 (≠ E368)
binding adenosine monophosphate: V235 (≠ Y265), L236 (≠ I266), S242 (= S272), S260 (= S286), M261 (= M287), Y314 (≠ A345), L318 (= L349), G335 (= G367), Y336 (≠ E368)
binding adenosine-5'-triphosphate: V235 (≠ Y265), L236 (≠ I266), S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272), S260 (= S286), M261 (= M287), L318 (= L349), G335 (= G367), D339 (= D371)
binding magnesium ion: D241 (= D271), D339 (= D371)
binding pyrophosphate 2-: S237 (= S267), G239 (= G269), D241 (= D271), S242 (= S272), D339 (= D371)

Sites not aligning to the query:

active site: 370, 431
binding adenosine-5'-triphosphate: 411, 431
binding pyrophosphate 2-: 411, 431

1jgtB Crystal structure of beta-lactam synthetase (see paper)
29% identity, 46% coverage: 74:385/684 of query aligns to 72:360/500 of 1jgtB

query
sites
1jgtB

F

L

N
x
A

G
|
G

E

E

I

I

F

Y

N

N

H

R

V

D

E

E

L

L

R

L

R

S

D

V

L

L

E

P

A

A

R

G

G

P

H

-

R

-

F

-

Y

A

T

P

R

E

T

G

D

D

T

A

E

E

V

L

I

V

V

L

H

R

A

L

F

L

E

E

E

R

Y

Y

G

D

P

L

A

H

T

A

W

F

A

R

R

L

L

V

N

N

G

G

Q

R

F

F

A

A

I

T

C

V

L

V

W

R

D

T

A

G

T

D

R

R

R

-

E

-

L

V

W

L

L

L

V

A

R

T

D

D

R

H

L

A

G

G

I

S

L

V

P

P

L

L

F

Y

Y

T

A

C

R

V

R

A

G

P

D

G

H

E

L

V

V

R

F

A

A

S

S

T

E

E

A

A

K

K

A

A

L

L

F

A

G

A

-

H

-

R

G

D

G

P

R

K

V

G

A

F

P

P

A

L

F

A

D

D

H

A

A

R

R

R

L

V

A

A

Q

G

V

-

F

-

T

-

H

-

W

-

S

-

A

-

P

-

H

-

G

-

S

-

V

-

F

L

E

T

R

G

V

V

E

Y

S

Q

V

V

P

P

A

A

A

G

T

A

A

V

I

M

R

D

V

I

D

D

-

L

-

G

-

S

S

G

T

T

L

A

A

V

L

T

H

H

V

-

D

-

R

R

Y

T

W

W

Q

T

P

P

D

G

F

L

T

S

A

R

D

R

P

-

A

-

L

-

A

-

K

I

L

L

S

P

L

E

D

G

E

E

A

A

A

V

D

A

R

A

L

V

E

R

E

A

K

A

L

L

L

E

D

K

A

A

I

V

R

A

L

Q

R

R

L

V

R

T

-

P

A

G

D

D

V

T

P

P

V

L

G

-

V

V

Y
x
V

I
x
L

S
|
S

G

G

G
|
G

L
x
I

D
|
D

S
|
S

S

S

V

G

I

V

A

A

L

C

A

A

R

H

K

R

-

A

-

G

-

E

L

L

D

D

T

T

T

V

S
|
S

M
|
M

H

G

S

-

F

-

G

-

V

-

R

-

F

-

T

T

D

D

A

T

G

S

F

-

D

N

E

E

T

F

P

R

Q

E

Q

A

R

R

L

A

V

V

A

V

G

D

H

H

V

L

G

R

T

T

G

R

H

H

H

R

D

E

I

I

L

T

C

I

G

P

P

T

R

T

D

E

I

L

Q

L

A

A

A

Q

L

L

P

P

D

Y

V

A

V

V

R

W

H

A

S

S

E

E

T

S

-

V

-

D

P

P

L
x
D

V

I

R

I

T

E

A
x
Y

P

L

A

L

P

P

L
|
L

F

T

L

A

L

L

S

Y

R

R

L

A

V

L

R

-

E

D

S

G

G

P

I

E

R

R

V

R

V

I

L

L

S

T

G
|
G

E
x
Y

G
|
G

A

A

D
|
D

E
x
I

W

P

L

L

A

G

G

G

Y

-

D

-

L
x
M

F

H

K

R

E

E

D

D

K

R

V

L

active site: A73 (≠ N75), G74 (= G76), D319 (≠ L341), Y345 (≠ E368)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ Y265), L245 (≠ I266), S246 (= S267), G248 (= G269), I249 (≠ L270), D250 (= D271), S251 (= S272), S269 (= S286), M270 (= M287), L327 (= L349), G344 (= G367), Y345 (≠ E368), D348 (= D371)
binding n2-(carboxyethyl)-l-arginine: Y323 (≠ A345), Y345 (≠ E368), G346 (= G369), D348 (= D371), I349 (≠ E372), M354 (≠ L379)
binding magnesium ion: D250 (= D271), D348 (= D371)

Sites not aligning to the query:

active site: 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 420, 440
binding n2-(carboxyethyl)-l-arginine: 370, 379

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
34% identity, 18% coverage: 44:165/684 of query aligns to 67:199/460 of 6lbpA

query
sites
6lbpA

I

I

G

A

L

I

A

G

H

H

A

V

R

R

L

Y

S

S

I

T

V

A

G

G

-

S

L

M

A

L

G

K

G

N

F

V

Q

Q

P

P

I

F

H

V

N

A

E

G

D

Y

R

R

-

F

-

G

S

S

L

V

W

G

I

V

S

A

F

H

N
|
N

G
|
G

E

N

I

L

F

V

N

N

H

Y

V

T

E

K

L

L

R

R

R

A

D

D

L

L

E

E

A

E

R

N

G

G

H

S

R

I

F

F

Y

N

T

T

R

S

T

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

-

L

-

I

A

A

F

I

E

S

E

K

Y

A

G

R

P

P

A

F

-

F

-

M

-

R

-

I

-

V

-

D

T

A

W

C

A

E

R

K

L

L

N

Q

G

G

Q

A

F

Y

A

S

I

M

C

V

L

F

W

-

D

-

A

V

T

T

R

E

R

D

E

K

L

L

W

V

L

A

V

V

R

R

D

D

R

P

L

H

G

G

I

F

L

R

P

P

L

L

F

V

Y

M

A

G

R

R

G

S

D

N

H

G

-

A

L

V

V

V

F

F

A

A

S

S

E

E

A

T

K

C

A

A

L

L

active site: N102 (= N75), G103 (= G76)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
34% identity, 18% coverage: 44:165/684 of query aligns to 153:285/561 of Q9STG9

query
sites
Q9STG9

I

I

G

A

L

I

A

G

H

H

A

V

R

R

L

Y

S

S

I

T

V

A

G

G

-

S

L

M

A

L

G

K

G

N

F

V

Q

Q

P

P

I

F

H

V

N

A

E

G

D

Y

R

R

-

F

-

G

S

S

L

V

W

G

I

V

S

A

F
x
H

N

N

G

G

E

N

I

L

F

V

N

N

H

Y

V

T

E

K

L

L

R

R

R

A

D

D

L

L

E

E

A

E

R

N

G

G

H

S

R

I

F

F

Y

N

T

T

R

S

T

S

D

D

T

T

E

E

V

V

I

V

V

L

H

H

-

L

-

I

A

A

F

I

E

S

E

K

Y

A

G

R

P

P

A

F

-

F

-

M

-

R

-

I

-

V

-

D

T

A

W

C

A

E

R

K

L

L

N

Q

G

G

Q

A

F

Y

A

S

I

M

C

V

L

F

W

-

D

-

A

V

T

T

R

E

R

D

E

K

L

L

W

V

L

A

V

V

R

R

D

D

R

P

L

H

G

G

I

F

L
x
R

P
|
P

L

L

F

V

Y

M

A

G

R

R

G

S

D

N

H

G

-

A

L

V

V

V

F

F

A

A

S

S

E

E

A

T

K

C

A

A

L

L

H187 (≠ F74) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ L145) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P146) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

1gph1 Structure of the allosteric regulatory enzyme of purine biosynthesis (see paper)
29% identity, 18% coverage: 46:165/684 of query aligns to 68:198/465 of 1gph1

query
sites
1gph1

L

I

A

G

H

H

A

V

R

R

L

Y

S

A

I

T

V

A

G

G

L

-

A

G

G

G

F

Y

Q

E

P

N

I

V

H

Q

-

P

-

L

-

F

-

R

-

S

N

Q

E

N

D

N

R

G

S

S

L

L

W

A

I

L

S

A

F

H

N

N

G

G

E

N

I

L

F

V

N

N

H

A

V

T

E

Q

L

L

R

K

R

Q

D

Q

L

L

E

E

A

N

R

Q

G

G

H

S

R

I

F

F

Y

Q

T

T

R

S

T

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

F

I

E

K

E

R

Y

S

G

G

P

H

A

F

T

T

-

L

-

K

-

D

-

Q

-

I

-

K

-

N

-

S

W

L

A

S

R

M

L

L

N

K

G

G

Q

A

F

Y

A

A

I

F

C

L

L

I

W

M

D

-

A

-

T

T

R

E

R

T

E

E

L

M

W

I

L

V

V

A

R

L

D

D

R

P

L

N

G

G

I

L

L

R

P

P

L

L

F

S

Y

I

A

G

R

M

G

G

D

D

H

A

L

Y

V

V

F

V

A

A

S

S

E

E

A

T

K

C

A

A

L

F

Sites not aligning to the query:

active site: 300, 305, 315, 423
binding adenosine monophosphate: 242, 242, 244, 245, 246, 282, 283, 283, 304, 305, 307, 345, 346, 347, 349, 350, 353, 388
binding iron/sulfur cluster: 236, 237, 382, 384, 388, 437, 440

P00497 Amidophosphoribosyltransferase; ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase; EC 2.4.2.14 from Bacillus subtilis (strain 168) (see 5 papers)
29% identity, 18% coverage: 46:165/684 of query aligns to 79:209/476 of P00497

query
sites
P00497

L

I

A

G

H

H

A

V

R

R

L

Y

S

A

I

T

V

A

G

G

L

-

A

G

G

G

F

Y

Q

E

P

N

I

V

H

Q

-

P

-

L

-

F

-

R

-

S

N

Q

E

N

D

N

R

G

S

S

L

L

W

A

I

L

S

A

F

H

N

N

G

G

E

N

I

L

F

V

N

N

H

A

V

T

E

Q

L

L

R

K

R

Q

D

Q

L

L

E

E

A

N

R

Q

G

G

H

S

R

I

F

F

Y

Q

T

T

R

S

T

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

F

I

E

K

E

R

Y

S

G

G

P

H

A

F

T

T

-

L

-

K

-

D

-

Q

-

I

-

K

-

N

-

S

W

L

A

S

R

M

L

L

N

K

G

G

Q

A

F

Y

A

A

I

F

C

L

L

I

W

M

D

-

A

-

T

T

R

E

R

T

E

E

L

M

W

I

L

V

V

A

R

L

D

D

R

P

L

N

G

G

I

L

L

R

P

P

L

L

F

S

Y

I

A

G

R

M

G

G

D

D

H

A

L

Y

V

V

F

V

A

A

S

S

E

E

A

T

K

C

A

A

L

F

Sites not aligning to the query:

1:11 modified: propeptide
12 active site, Nucleophile; C→F: Loss of enzyme activity and N-terminal processing.
247 binding [4Fe-4S] cluster
294 binding Mg(2+)
356 binding Mg(2+)
357 binding Mg(2+)
393 binding [4Fe-4S] cluster
394 F→V: Partial loss of activity.
442 D→S: Partial loss of activity.
448 binding [4Fe-4S] cluster; C→S: Loss of activity.
451 binding [4Fe-4S] cluster; C→S: Loss of activity.
452 F→C: Lethal.

1ao0A Glutamine phosphoribosylpyrophosphate (prpp) amidotransferase from b. Subtilis complexed with adp and gmp (see paper)
31% identity, 15% coverage: 66:165/684 of query aligns to 89:194/455 of 1ao0A

query
sites
1ao0A

E

N

D

N

R

G

S

S

L

L

W

A

I

L

S

A

F

H

N
|
N

G
|
G

E

N

I

L

F

V

N

N

H

A

V

T

E

Q

L

L

R

K

R

Q

D

Q

L

L

E

E

A

N

R

Q

G

G

H

S

R

I

F

F

Y

Q

T

T

R

S

T

S

D

D

T

T

E

E

V

V

I

L

V

A

H

H

A

L

F

I

E

K

E

R

Y

S

G

G

P

H

A

F

T

T

-

L

-

K

-

D

-

Q

-

I

-

K

-

N

-

S

W

L

A

S

R

M

L

L

N

K

G

G

Q

A

F

Y

A

A

I

F

C

L

L

I

W

M

D

-

A

-

T

T

R

E

R

T

E

E

L

M

W

I

L

V

V

A

R

L

D

D

R

P

L

N

G

G

I

L

L

R

P

P

L

L

F

S

Y

I

A

G

R

M

G

G

D

D

H

A

L

Y

V

V

F

V

A

A

S

S

E

E

A

T

K

C

A

A

L

F

active site: N98 (= N75), G99 (= G76)

Sites not aligning to the query:

active site: 1, 27, 238, 296, 301, 311, 419
binding guanosine-5'-monophosphate: 234, 238, 279, 341, 342, 343, 345, 346, 347, 348, 349
binding adenosine-5'-diphosphate: 25, 238, 240, 241, 242, 277, 278, 279, 300, 301, 301, 303
binding magnesium ion: 279, 341, 342
binding iron/sulfur cluster: 232, 233, 234, 378, 380, 433, 436

Query Sequence

>WP_012976121.1 NCBI__GCF_000010725.1:WP_012976121.1
MCGVAGVLAGSRAEPAGLDELKRMIAMLSHRGPDGYGFYRDDRIGLAHARLSIVGLAGGF
QPIHNEDRSLWISFNGEIFNHVELRRDLEARGHRFYTRTDTEVIVHAFEEYGPATWARLN
GQFAICLWDATRRELWLVRDRLGILPLFYARRGDHLVFASEAKALFGGGRVAPAFDHARL
AQVFTHWSAAPHGSVFERVESVPAATAIRVDSTLALHVDRYWQPDFTADPALAKLSLDEA
ADRLEEKLLDAIRLRLRADVPVGVYISGGLDSSVIAALARKLDTTSMHSFGVRFTDAGFD
ETPQQRLVAGHVGTGHHDILCGPRDIQAALPDVVRHSETPLVRTAPAPLFLLSRLVRESG
IRVVLSGEGADEWLAGYDLFKEDKVRRFWARQPGSKVRPKLLGRVHPFAAVNGQKDSPLW
QAFFKRGMMETDEAFYAHRTRWRNTAWAQRLLSADVQATADDAAFEAHVAAHLPDGWSRW
SPLARSQWAEISSFMTPYLLSTQGDRVAMANGIEVRYPFLDPAVDDLCAALPDRVKMLGL
RDKLALRRLAARHLPAEIVQRPKRPYRAPMTSALFGDAAPDHVRDLLSPDALVRYGLTDA
TAVLALADKAHRRNGHMAGEREEMALVGALTLQMLAHYIQDDLPQRIRDQRAALDRAEIH
VLEDQALKDRAAGSRTATPQPTAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory