SitesBLAST
Comparing WP_012976903.1 NCBI__GCF_000010725.1:WP_012976903.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5g4jA Phospholyase a1rdf1 from arthrobacter in complex with phosphoethanolamine (see paper)
44% identity, 95% coverage: 22:438/438 of query aligns to 2:421/423 of 5g4jA
- active site: S14 (≠ Y34), Y130 (= Y150), D201 (= D218), D234 (= D251), Q237 (= Q254), K264 (= K281), T294 (= T311), K395 (= K412)
- binding {5-hydroxy-6-methyl-4-[(E)-{[2-(phosphonooxy)ethyl]imino}methyl]pyridin-3-yl}methyl dihydrogen phosphate: Y44 (= Y64), R73 (= R93), G103 (= G123), S104 (= S124), Y130 (= Y150), H131 (= H151), D234 (= D251), V236 (= V253), Q237 (= Q254), K264 (= K281), T294 (= T311), R397 (= R414)
5g4iA Plp-dependent phospholyase a1rdf1 from arthrobacter aurescens tc1 (see paper)
44% identity, 95% coverage: 22:438/438 of query aligns to 2:421/423 of 5g4iA
- active site: S14 (≠ Y34), Y130 (= Y150), D201 (= D218), D234 (= D251), Q237 (= Q254), K264 (= K281), T294 (= T311), K395 (= K412)
- binding pyridoxal-5'-phosphate: G103 (= G123), S104 (= S124), H131 (= H151), D234 (= D251), V236 (= V253), Q237 (= Q254), K264 (= K281)
- binding phosphate ion: Y44 (= Y64), R397 (= R414)
6torB Human o-phosphoethanolamine phospho-lyase (see paper)
45% identity, 91% coverage: 42:438/438 of query aligns to 1:397/404 of 6torB
Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
34% identity, 90% coverage: 44:436/438 of query aligns to 31:395/395 of Q5SHH5
- GT 113:114 (≠ GS 123:124) binding pyridoxal 5'-phosphate
- K254 (= K281) modified: N6-(pyridoxal phosphate)lysine
- T283 (= T311) binding pyridoxal 5'-phosphate
1zobA Crystal structure of dialkylglycine decarboxylases bound with calcium ion
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1zobA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding calcium ion: L76 (≠ T90), S78 (≠ T92), V303 (≠ G313), S304 (≠ G314), D305 (≠ N315)
- binding pyridoxal-5'-phosphate: T108 (= T122), A110 (≠ S124), N113 (= N127), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281)
Sites not aligning to the query:
1zc9A The crystal structure of dialkylglycine decarboxylase complex with pyridoxamine 5-phosphate (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1zc9A
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281)
Sites not aligning to the query:
1m0qA Structure of dialkylglycine decarboxylase complexed with s-1- aminoethanephosphonate (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1m0qA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding (1s)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]ethylphosphonic acid: Q50 (≠ V67), T108 (= T122), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), S213 (= S223), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
1m0pA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- phenylethanephosphonate (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1m0pA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-phenylethylphosphonic acid: Q50 (≠ V67), T108 (= T122), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), S213 (= S223), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
1m0oA Structure of dialkylglycine decarboxylase complexed with 1-amino-1- methylpropanephosphonate (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1m0oA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding (1r)-1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]-1-methylpropylphosphonic acid: Q50 (≠ V67), G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
1m0nA Structure of dialkylglycine decarboxylase complexed with 1- aminocyclopentanephosphonate (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1m0nA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding 1-[((1e)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylene)amino]cyclopentylphosphonic acid: Q50 (≠ V67), M51 (≠ A68), A110 (≠ S124), N113 (= N127), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), S213 (= S223), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
32% identity, 92% coverage: 34:435/438 of query aligns to 24:447/448 of 6io1B
- active site: Y151 (= Y150), D257 (= D251), K286 (= K281)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ T122), G118 (= G123), A119 (≠ S124), N122 (= N127), Y151 (= Y150), H152 (= H151), D257 (= D251), V259 (= V253), I260 (≠ Q254), K286 (= K281)
Sites not aligning to the query:
1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 90% coverage: 44:436/438 of query aligns to 23:387/387 of 1wkhA
- active site: F132 (≠ Y150), E184 (≠ D218), D217 (= D251), Q220 (= Q254), K246 (= K281), T275 (= T311), R363 (≠ K412)
- binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (vs. gap), S104 (≠ T122), G105 (= G123), T106 (≠ S124), F132 (≠ Y150), S133 (≠ H151), E184 (≠ D218), E189 (≠ S223), D217 (= D251), I219 (≠ V253), K246 (= K281), R363 (≠ K412)
Sites not aligning to the query:
1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 90% coverage: 44:436/438 of query aligns to 23:387/387 of 1wkgA
- active site: F132 (≠ Y150), E184 (≠ D218), D217 (= D251), Q220 (= Q254), K246 (= K281), T275 (= T311), R363 (≠ K412)
- binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (vs. gap), G105 (= G123), T106 (≠ S124), F132 (≠ Y150), S133 (≠ H151), R135 (≠ V153), E184 (≠ D218), D217 (= D251), I219 (≠ V253), Q220 (= Q254), K246 (= K281), G273 (≠ F309), T274 (≠ N310), T275 (= T311)
Sites not aligning to the query:
1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
34% identity, 90% coverage: 44:436/438 of query aligns to 23:387/387 of 1vefA
- active site: F132 (≠ Y150), D217 (= D251), K246 (= K281), T275 (= T311), R363 (≠ K412)
- binding pyridoxal-5'-phosphate: G105 (= G123), T106 (≠ S124), F132 (≠ Y150), S133 (≠ H151), E184 (≠ D218), D217 (= D251), I219 (≠ V253), K246 (= K281)
Sites not aligning to the query:
1d7vA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with nma (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1d7vA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding potassium ion: H75 (vs. gap), L76 (≠ T90), F77 (≠ H91), S78 (≠ T92), T301 (= T311), H302 (≠ F312), V303 (≠ G313), S304 (≠ G314), D305 (≠ N315)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-2-methylalanine: A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), S213 (= S223), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
1d7uA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with lcs (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1d7uA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding potassium ion: H75 (vs. gap), L76 (≠ T90), F77 (≠ H91), S78 (≠ T92), T301 (= T311), H302 (≠ F312), V303 (≠ G313), S304 (≠ G314), D305 (≠ N315)
- binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
Sites not aligning to the query:
1d7sA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with dcs (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1d7sA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: Q50 (≠ V67), G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
- binding potassium ion: H75 (vs. gap), L76 (≠ T90), F77 (≠ H91), S78 (≠ T92), T301 (= T311), H302 (≠ F312), V303 (≠ G313), S304 (≠ G314), D305 (≠ N315)
Sites not aligning to the query:
1d7rA Crystal structure of the complex of 2,2-dialkylglycine decarboxylase with 5pa (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1d7rA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), S213 (= S223), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281), R404 (≠ K412)
- binding potassium ion: H75 (vs. gap), L76 (≠ T90), F77 (≠ H91), S78 (≠ T92), T301 (= T311), H302 (≠ F312), V303 (≠ G313), S304 (≠ G314), D305 (≠ N315)
Sites not aligning to the query:
1dgdA An alkali metal ion size-dependent switch in the active site structure of dialkylglycine decarboxylase (see paper)
32% identity, 90% coverage: 40:435/438 of query aligns to 23:427/431 of 1dgdA
- active site: W136 (≠ Y150), E208 (≠ D218), D241 (= D251), Q244 (= Q254), K270 (= K281), T301 (= T311), R404 (≠ K412)
- binding pyridoxal-5'-phosphate: T108 (= T122), G109 (= G123), A110 (≠ S124), W136 (≠ Y150), H137 (= H151), E208 (≠ D218), D241 (= D251), A243 (≠ V253), Q244 (= Q254), K270 (= K281)
Sites not aligning to the query:
5m4bA Alpha-amino epsilon-caprolactam racemase d210a mutant in complex with plp and geminal diamine intermediate
32% identity, 98% coverage: 9:438/438 of query aligns to 3:425/439 of 5m4bA
- active site: Q21 (≠ E27), Y140 (= Y150), E208 (≠ D218), D241 (= D251), K244 (≠ Q254), K270 (= K281), T298 (= T311), E399 (≠ K412)
- binding [6-methyl-5-oxidanyl-4-[(~{E})-[(3~{R})-2-oxidanylideneazepan-3-yl]iminomethyl]pyridin-3-yl]methyl dihydrogen phosphate: W53 (vs. gap), S112 (≠ T122), G113 (= G123), S114 (= S124), Y140 (= Y150), H141 (= H151), E208 (≠ D218), D241 (= D251), V243 (= V253), K244 (≠ Q254), K270 (= K281)
Query Sequence
>WP_012976903.1 NCBI__GCF_000010725.1:WP_012976903.1
MTMINAYAPDAAAPLPDRERALIARREKLLGPAYRLFYADPVHVVRGEGASLYDADGNRY
LDAYNNVASVGHCRPEVVEAMARQAAVLNTHTRYLTDGILDFAEALLAEFPAGLSHLMMT
CTGSEANDLALRVARVHTGGTGVVIAHNAYHGVTSALAEMSPSLGAAVKLGDHVRVVPSP
DGYRIAGSDVGAAFARSVEAAIADLREKGIAPAALLVDTIFSSSGVYTDPAGFLAPAVEV
MRKAGGLFIADEVQPGFGRLGTHMWGFARHGLVPDIVTLGKPMGNGHPVAGAVFRPEVIE
AFGKNQRYFNTFGGNPVSCAVAHAVLRVIKTERLQENALAVGTRMLDGLRALAAKHDLIG
DVRGSGLFIGVEMVRDRALKTPAPEETARVVNDMRRRRVLISATGQEGHILKIRPPLVFS
AQDAELFLATLDEVLAAL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory