SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012977188.1 NCBI__GCF_000010725.1:WP_012977188.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
35% identity, 96% coverage: 16:446/447 of query aligns to 40:471/472 of P78061

query
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P78061

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H282 (= H257) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R333) mutation to Q: Activity is impaired to 3% of wild-type.

8ufjB Glutamine synthetase (see paper)
30% identity, 98% coverage: 8:444/447 of query aligns to 14:441/444 of 8ufjB

query
sites
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binding magnesium ion: E132 (= E136), E134 (= E138), E189 (= E201), E196 (= E208), H245 (= H257), E333 (= E345)

8tfkA Glutamine synthetase (see paper)
30% identity, 98% coverage: 8:444/447 of query aligns to 10:437/440 of 8tfkA

query
sites
8tfkA

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L

L

P

P

R

A

H

D

Q

L

S

K

E

E

A

A

L

I

S

D

K

E

F

M

N

K

A

G

C

S

K

K

P

F

L

V

K

K

E

E

I

A

L

L

G

G

E

E

R

H

F

V

I

F

D

S

A

H

V

Y

D

L

C

C

V

A

K

K

Q

E

A

M

E

E

Y

W

K

D

A

E

Y

Y

N

K

R

A

V

V

I

V

S

H

S

P

W

W

E

E

R

L

E

S

N

R

L

Y

L

L

binding adenosine-5'-diphosphate: E128 (= E136), D194 (≠ N210), F195 (= F211), F197 (≠ H213), N243 (≠ H259), R312 (= R328), R317 (= R333), G325 (≠ A341), R327 (= R343)
binding magnesium ion: E128 (= E136), E128 (= E136), E130 (= E138), E185 (= E201), E192 (= E208), E192 (= E208), H241 (= H257), E329 (= E345)
binding l-methionine-s-sulfoximine phosphate: E128 (= E136), E130 (= E138), E185 (= E201), E192 (= E208), G237 (= G253), H241 (= H257), R294 (= R310), E300 (≠ D316), R312 (= R328), R331 (= R347)

8oozA Glutamine synthetase (see paper)
31% identity, 85% coverage: 66:444/447 of query aligns to 50:427/430 of 8oozA

query
sites
8oozA

D

D

I

G

T

S

S

S

D

I

E

E

N

G

S

S

D

D

I

M

Y

V

M

L

I

R

P

P

D

D

E

P

R

D

T

T

I

F

R

R

F

V

V

L

P

P

W

W

Y

S

T

T

E

A

P

-

T

E

A

A

Q

R

V

L

I

I

T

C

D

D

C

I

V

E

Y

L

A

P

D

D

G

G

R

K

P

P

V

F

D

M

V

G

S

C

P

P

R

R

H

Q

V

V

L

L

K

K

R

K

V

N

L

M

S

E

L

E

Y

A

E

A

E

K

R

L

G

G

W

Y

K

V

P

M

L

N

V

T

A
x
G

P

P

E
|
E

L

M

E

E

F

F

L

L

V

F

Q

K

V

-

N

-

K

R

D

Q

P

D

D

G

Y

M

P

P

L

T

V

N

P

I

P

P

V

Q

G

D

R

R

N

G

G

G

R

Y

M

-

E

-

S

-

G

-

R

-

Q

-

A

-

F

F

G

D

I

L

D

A

A

P

V

I

N

D

E

L

F

A

D

E

P

E

I

I

F

K

E

R

A

E

V

I

Y

V

D

L

F

V

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

V

D
x
E

T

A

L

A

T

H

H

H

E

E

A

V

G

A

A

F

A

G

Q

Q

I

H

E

E

I

I

N

D

F
|
F

N
x
K

H
x
Y

G

D

D

N

A

A

L

L

E

A

L

T

A

A

D

D

Q

N

A

V

F

I

L

T

F

L

K

K

R

Y

T

V

A

A

R

K

E

T

A

L

A

A

I

L

R

Q

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

Q

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H
|
H

I

T

H
x
N

Q

T

S
|
S

V

L

V

F

D

K

A

-

D

-

S

D

G

G

R

K

N

N

L

A

F

F

A

Y

N

D

P

P

D

D

G

A

T

P

D

D

-

Q

-

I

S

S

P

D

L

T

F

L

L

R

S

Y

H

F

I

V

A

G

G

G

L

V

Q

L

K

K

Y

H

L

I

P

R

Y

A

A

I

M

T

P

A

L

I

L

T

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

P

N

G

S

Y

D

E

A

A

P

P

I

V

N

Y

V

I

H

T

W

W

G

S

R

G

D

P

N

N

R

R

T

S

G

L

L

I

R

R

V

V

P

P

V

A

S

P

P

R

P

G

D

N

A
x
S

R

T

R
|
R

V

I

E
|
E

N

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

S

I

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

V

T

K

Q

N

G

K

L

I

E

E

P

P

N

P

D

E

P

R

I

V

K

E

G

K

S

N

A

I

Y

Y

R

K

L

L

A

T

-

E

-

R

-

E

-

K

-

L

-

G

-

I

F

G

T

M

L

L

P

P

R

G

H

T

Q

L

S

K

E

E

A

A

L

I

S

E

K

C

F

F

N

K

A

E

C

D

K

E

P

L

L

L

K

V

E

S

I

A

L

L

G

G

E

E

R

H

F

V

I

S

D

Q

A

S

V

I

D

I

C

N

V

V

K

A

Q

M

A

A

E

D

Y

W

K

D

A

S

Y

Y

N

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

L

E

D

N

R

L

Y

L

L

binding adenosine-5'-triphosphate: G117 (≠ A134), E170 (≠ D196), F185 (= F211), K186 (≠ N212), Y187 (≠ H213), N233 (≠ H259), S235 (= S261), S315 (≠ A341), R317 (= R343)
binding magnesium ion: E119 (= E136), H231 (= H257), E319 (= E345)

8ooxB Glutamine synthetase (see paper)
31% identity, 84% coverage: 71:444/447 of query aligns to 61:435/438 of 8ooxB

query
sites
8ooxB

E

E

N

E

S

S

D

D

I

M

Y

V

M

L

I

R

P

P

D

D

E

P

R

D

T

T

I

F

R

R

F

V

V

L

P

P

W

W

Y

S

T

G

E

N

P

T

T

A

-

E

A

A

Q

R

V

L

I

I

T

C

D

D

C

I

V

E

Y

L

A

P

D

D

G

G

R

K

P

P

V

F

D

M

V

G

S

C

P

P

R

R

H

Q

V

V

L

L

K

K

R

K

V

N

L

M

S

E

L

E

Y

A

E

A

E

K

R

L

G

G

W

Y

K

V

P

M

L

N

V

T

A

G

P

P

E

E

L

M

E
|
E

F

F

L

L

V

F

Q

K

V

-

N

-

K

R

D

Q

P

D

D

G

Y

M

P

P

L

T

V

N

P

I

P

P

V

Q

G

D

R

R

N

G

G

G

R

Y

M

-

E

-

S

-

G

-

R

-

Q

-

A

-

F

F

G

D

I

L

D

A

A

P

V

I

N

D

E

L

F

A

D

E

P

E

I

I

F

K

E

R

A

E

V

I

Y

V

D

L

F

V

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

V

D

E

T

A

L

A

T

H

H

H

E
|
E

A

V

G

A

A

F

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F

F

N

K

H

Y

G

D

D

N

A

A

L

L

E

A

L

T

A

A

D

D

Q

N

A

V

F

I

L

T

F

L

K

K

R

Y

T

V

A

A

R

K

E

T

A

L

A

A

I

L

R

Q

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

I

Q

F

G

G

E

V

P

N

G

G

S

S

A

G

M

M

H

H

I

T

H

N

Q

T

S

S

V

L

V

F

D

K

A

-

D

-

S

D

G

G

R

K

N

N

L

A

F

F

A

Y

N

D

P

P

D

D

G

A

T

P

D

D

-

Q

-

I

S

S

P

D

L

T

F

L

L

R

S

Y

H

F

I

V

A

G

G

G

L

V

Q

L

K

K

Y

H

L

I

P

R

Y

A

A

I

M

T

P

A

L

I

L

T

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

P

N

G

S

Y

D

E

A

A

P

P

I

V

N

Y

V

I

H

T

W

W

G

S

R

G

D

P

N

N

R

R

T

S

G

L

L

I

R

R

V

V

P

P

V

A

S

P

P

R

P

G

D

N

A

S

R

T

R

R

V

I

E

E

N

I

R

R

V

S

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

A

S

I

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

V

T

K

Q

N

G

K

L

I

E

E

P

P

N

P

D

E

P

R

I

V

K

E

G

K

S

N

A

I

Y

Y

R

K

L

L

A

T

-

E

-

R

-

E

-

K

-

L

-

G

-

I

F

G

T

M

L

L

P

P

R

G

H

T

Q

L

S

K

E

E

A

A

L

I

S

E

K

C

F

F

N

K

A

E

C

D

K

E

P

L

L

L

K

V

E

S

I

A

L

L

G

G

E

E

R

H

F

V

I

S

D

Q

A

S

V

I

D

I

C

N

V

V

K

A

Q

M

A

A

E

D

Y

W

K

D

A

S

Y

Y

N

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

L

E

D

N

R

L

Y

L

L

binding magnesium ion: E129 (= E138), E183 (= E201), E190 (= E208)

8oooA Glutamine synthetase from methanothermococcus thermolithotrophicus in complex with 2-oxoglutarate and mgatp at 2.15 a resolution (see paper)
28% identity, 98% coverage: 6:444/447 of query aligns to 8:445/447 of 8oooA

query
sites
8oooA

D

E

F

Y

I

V

K

K

K

S

N

N

R

N

I

V

T

K

E
x
F

V

M

E
x
R

C

F

L

Q

V

F

P

V

D

D

M

I

S

L

G

G

I

V

A

P

R

K

G

N

K

V

I
x
A

V

F

P

P

-

I

-

K

-

A

A

G

E

E

K

K

F

G

L

I

R

E

I

E

L

L

R

R

D

D

R

-

G

-

L

-

R

-

L

-

P

-

E

-

A

-

I

V

F

L

V

E

Q

N

T

G

V

L

T

Y

G

F

E

D

F

G

P

S

D

S

D

I

E

E

D

G

I

F

T

V

S

G

D

I

E

N

N

E

S

S

D

D

I

M

Y

M

M

L

I

K

P

P

D

D

E

L

R

S

T

T

I

F

R

S

F

V

V

L

P

P

W

W

-
x
R

-

P

Y

S

T

E

E

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

T

C

D

D

C

V

V

Y

Y

T

A

T

D

K

G

G

R

K

P

P

V

F

D

E

V

G

S

D

P

P

R

R

H

G

V

C

L

L

K

K

R

R

V

V

L

M

S

E

L

E

Y

F

E

K

-

K

E

E

R

F

G

N

W

G

K

E

P

Y

L

F

V

V

A

G

P

P

E
|
E

L

P

E

E

F

F

L

L

V

L

Q

K

V

-

N

-

K

K

D

D

P

P

D

H

Y

N

P

P

L

H

V

K

P

Y

P

I

V

P

G

A

R

D

N

D

G

G

R

G

M

Y

E

-

S

-

G

-

R

-

Q

-

A

-

F

F

G

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

I

I

F
x
R

E
x
R

A

D

V

I

Y

V

D

F

F

A

C

L

E

E

K

N

Q

L

D

G

I

F

D

H

I

V

D
x
E

T

A

L
x
S

T

H

H

H

E

E

A

V

G

A

A

P

A

G

Q

Q

I

H

E

E

I

V

N

D

F
|
F

N
x
K

H
x
F

G

D

D

D

A

A

L

L

E

K

L

T

A

A

D

D

Q

S

A

V

F

I

L

T

F

F

K

K

R

T

T

T

A

I

R

K

E

T

A

I

A

A

I

E

R

Q

H

H

Q

G

V

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

F

Q

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H
|
H

Q

Q

S
|
S

V

I

V

W

D

L

A

N

D

G

S

E

G

P

R

S

N

F

L

Y

F

D

A

E

N

N

P

A

D

P

G

Y

T

Q

D

L

S

S

P

E

L

T

F

C

L

M

S

N

H

Y

I

V

A

A

G

G

L

I

Q

L

K

K

Y

H

L

A

P

K

Y

A

A

I

M

V

P

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

P

N

G

S

Y

D

E

A

A

P

P

I

V

N

N

V

I

H

A

W

W

G

A

R

N

D

S

N

N

R

R

T

S

T

A

G

I

L

I

R
|
R

V

V

P

P

V

A

S

A

P

R

P

G

D

K

A

G

R

T

R
|
R

V

I

E

E

N

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

T

A

V

S

M

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

V

T

K

Q

N

G

K

L

L

E

D

P

A

N

P

D

E

P

P

I

V

K

E

G

R

S

N

A

I

Y

F

R

A

L

M

A

S

-

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

F

E

T

S

L

V

P

P

R

A

H

N

Q

L

S

K

E

A

A

A

L

L

S

D

K

E

F

L

N

E

A

N

C

N

K

D

P

V

L

L

K

K

E

N

I

A

L

L

G

G

E

K

R

H

F

I

I

F

D

E

A

S

V

F

D

L

C

E

V

I

K

K

Q

N

A

A

E

E

Y

W

K

D

A

S

Y

F

N

R

R

T

V

S

I

V

S

T

S

D

W

W

E

E

R

T

E

T

N

A

L

Y

L

L

binding 2-oxoglutaric acid: F17 (≠ E15), R19 (≠ E17), A33 (≠ I31), R87 (vs. gap), V93 (≠ T94), P170 (≠ D178), R173 (≠ F181), R174 (≠ E182), S190 (≠ L198)
binding adenosine-5'-triphosphate: E136 (= E136), E188 (≠ D196), F203 (= F211), K204 (≠ N212), F205 (≠ H213), H251 (= H259), S253 (= S261), R325 (= R333), R335 (= R343)

8ooqB Glutamine synthetase from Methanothermococcus thermolithotrophicus (see paper)
28% identity, 98% coverage: 6:444/447 of query aligns to 7:444/446 of 8ooqB

query
sites
8ooqB

D

E

F

Y

I

V

K

K

K

S

N

N

R

N

I

V

T

K

E
x
F

V

M

E
x
R

C

F

L

Q

V

F

P

V

D

D

M

I

S

L

G

G

I

V

A

P

R

K

G

N

K

V

I
x
A

V

F

P

P

-

I

-

K

-

A

A

G

E

E

K

K

F

G

L

I

R

E

I

E

L

L

R

R

D

D

R

-

G

-

L

-

R

-

L

-

P

-

E

-

A

-

I

V

F

L

V

E

Q

N

T

G

V

L

T

Y

G

F

E

D

F

G

P

S

D

S

D

I

E

E

D

G

I

F

T

V

S

G

D

I

E

N

N

E

S

S

D

D

I

M

Y

M

M

L

I

K

P

P

D

D

E

L

R

S

T

T

I

F

R

S

F

V

V

L

P

P

W

W

-
x
R

-

P

Y

S

T

E

E

K

P

S

T
x
V

A

A

Q

R

V

V

I

I

T

C

D

D

C

V

V

Y

Y

T

A

T

D

K

G

G

R

K

P

P

V

F

D

E

V

G

S

D

P

P

R

R

H

G

V

C

L

L

K

K

R

R

V

V

L

M

S

E

L

E

Y

F

E

K

-

K

E

E

R

F

G

N

W

G

K

E

P

Y

L

F

V

V

A

G

P

P

E

E

L

P

E
|
E

F

F

L

L

V

L

Q

K

V

-

N

-

K

K

D

D

P

P

D

H

Y

N

P

P

L

H

V

K

P

Y

P

I

V

P

G

A

R

D

N

D

G

G

R

G

M

Y

E

-

S

-

G

-

R

-

Q

-

A

-

F

F

G

D

I

L

D

E

A

P

V

M

N

D

E

E

F

A

D
x
P

P

D

I

I

F
x
R

E
x
R

A

D

V

I

Y

V

D

F

F

A

C

L

E

E

K

N

Q

L

D

G

I

F

D

H

I

V

D

E

T

A

L
x
S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

V

N

D

F

F

N

K

H

F

G

D

D

D

A

A

L

L

E

K

L

T

A

A

D

D

Q

S

A

V

F

I

L

T

F

F

K

K

R

T

T

T

A

I

R

K

E

T

A

I

A

A

I

E

R

Q

H

H

Q

G

V

L

Y

K

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

F

Q

F

G

G

E

M

P

N

G

G

S

S

A

G

M

M

H

H

I

C

H

H

Q

Q

S

S

V

I

V

W

D

L

A

N

D

G

S

E

G

P

R

S

N

F

L

Y

F

D

A

E

N

N

P

A

D

P

G

Y

T

Q

D

L

S

S

P

E

L

T

F

C

L

M

S

N

H

Y

I

V

A

A

G

G

L

I

Q

L

K

K

Y

H

L

A

P

K

Y

A

A

I

M

V

P

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

P

N

G

S

Y

D

E

A

A

P

P

I

V

N

N

V

I

H

A

W

W

G

A

R

N

D

S

N

N

R

R

T

S

T

A

G

I

L

I

R

R

V

V

P

P

V

A

S

A

P

R

P

G

D

K

A

G

R

T

R

R

V

I

E

E

N

F

R

R

V

A

A

P

G

D

A

P

D

S

A

C

N

N

P

P

Y

Y

L

L

A

A

I

F

A

T

A

V

S

M

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

V

T

K

Q

N

G

K

L

L

E

D

P

A

N

P

D

E

P

P

I

V

K

E

G

R

S

N

A

I

Y

F

R

A

L

M

A

S

-

E

-

A

-

E

-

K

-

E

-

L

-

G

-

I

F

E

T

S

L

V

P

P

R

A

H

N

Q

L

S

K

E

A

A

A

L

L

S

D

K

E

F

L

N

E

A

N

C

N

K

D

P

V

L

L

K

K

E

N

I

A

L

L

G

G

E

K

R

H

F

I

I

F

D

E

A

S

V

F

D

L

C

E

V

I

K

K

Q

N

A

A

E

E

Y

W

K

D

A

S

Y

F

N

R

R

T

V

S

I

V

S

T

S

D

W

W

E

E

R

T

E

T

N

A

L

Y

L

L

binding 2-oxoglutaric acid: F16 (≠ E15), R18 (≠ E17), A32 (≠ I31), R86 (vs. gap), V92 (≠ T94), P169 (≠ D178), R172 (≠ F181), R173 (≠ E182), S189 (≠ L198)
binding magnesium ion: E137 (= E138), E192 (= E201), E199 (= E208)

7tfaB Glutamine synthetase (see paper)
33% identity, 84% coverage: 71:444/447 of query aligns to 62:438/441 of 7tfaB

query
sites
7tfaB

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

Y

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

V

V

F

P

P

W

W

Y

V

T

T

-

S

E

D

P

R

T

V

A

A

Q

R

V

L

I

I

T

C

D

D

C

I

V

Y

Y

K

A

P

D

D

G

G

R

S

P

P

V

F

D

A

V

G

S

D

P

P

R

R

H

G

V

I

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

A

E

E

R

L

G

G

W

Y

K

T

P

S

L

M

-

N

V

V

A

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

T

N

D

K

E

D

K

P

G

D

D

Y

-

P

P

L

T

V

T

P

E

P

L

V

N

G

D

R

Q

N

G

G

G

R
x
Y

M

F

E

D

S

L

G

A

R

P

Q

M

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

E

F

-

D

-

P

-

I

-

F

-

E

-

A

-

V

I

Y

V

D

L

F

K

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D

E

T

A

L

S

T

H

H

H

E
|
E

A
x
V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F

F

N

K

H

Y

G

A

D

D

A

A

L

V

E

K

L

A

A

A

D

D

Q

Q

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

Q

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

Q

S

S

V

L

V

F

D

K

A

-

D

-

S

D

G

N

R

E

N

N

L

V

F

F

A

Y

N

-

P

-

D

D

G

E

T

T

D

D

S

E

P

-

L

L

F

G

L

L

S

S

-

Q

-

T

-

A

-

R

-

H

H

Y

I

M

A

A

G

G

L

I

Q

L

K

K

Y

H

L

A

P

R

Y

A

A

M

M

A

P

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

V

V

P

P

N
x
G

S
x
Y

D
x
E

A

A

P

P

I

C

N

Y

V

V

H

A

W

W

G

S

R

A

D

S

N

N

R
|
R

T

S

T

P

G

M

L

I

R

R

V

I

P

P

V

A

S

S

P

R

P

G

D

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

A

V

S

M

L

L

A

R

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

R

G

Q

L

M

E

A

P

L

N

P

D

A

P

P

I

I

K

D

G

R

S

N

A

I

Y

Y

R

V

L

M

A

S

F

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

T

S

L

L

P

P

R

A

H

D

Q

L

S

K

E

E

A

A

L

L

S

S

K

E

F

L

N

I

A

R

C

S

K

E

P

V

L

I

K

S

E

D

I

A

L

L

G

G

E

D

R

H

F

A

I

L

D

A

A

Y

V

F

D

Y

C

E

V

L

K

K

Q

E

A

I

E

E

Y

W

K

D

A
x
M

Y

Y

N

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

E

D

N

Q

L

Y

L

L

binding glutamine: E131 (= E138), Y153 (≠ R161), E186 (= E201), G238 (= G253), H242 (= H257), R295 (= R310), E301 (≠ D316)
binding magnesium ion: E129 (= E136), E131 (= E138), E186 (= E201), E193 (= E208), H242 (= H257), E330 (= E345)
binding : V187 (≠ A202), N237 (≠ P252), G299 (≠ N314), Y300 (≠ S315), R313 (= R328), M424 (≠ A430)

Sites not aligning to the query:

binding : 58, 60

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
29% identity, 99% coverage: 4:444/447 of query aligns to 8:440/443 of 4lnkA

query
sites
4lnkA

M

I

E

E

D

K

F

L

I

V

K

K

K

E

N

E

R

N

I

V

T

K

E

Y

V

I

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

S

L

G

G

I

T

A

I

R

K

G

N

K

V

I

E

V

I

P

P

A

V

E

S

K

Q

F

L

L

G

R
x
K

I

A

L

L

R

D

D

N

R

K

G

V

L
x
M

R

F

L
x
D

P

G

E

S

A

S

I

I

F

-

V

-

Q

-

T

-

V

-

T

-

G

-

E

-

F

-

P

-

D

-

E

E

D

G

I

F

T

V

S

R

D

I

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

Y

P

P

D

D

E

L

R

N

T

T

I

F

R

V

F

I

V

F

P

P

W

W

Y

T

T

A

E

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

F

I

I

T

C

D

D

C

I

V

Y

Y

N

A

P

D

D

G

G

R

T

P

P

V

F

D

E

V

G

S

D

P

P

R

R

H

N

V

N

L

L

K

K

R

R

V

I

L

L

S

K

L

E

Y

M

E

E

E

D

R

L

G

G

W

F

K

S

P

D

L

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

L

N

D

K

E

D

K

P

G

D

E

Y

-

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

K

N

G

G

G

R

Y

M

F

E

D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D

E

T

A

L

S

T

H

H

H

E
|
E

A
x
V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F
|
F

N

K

H
x
Y

G

A

D

G

A

A

L

V

E

R

L

S

A

C

D

D

Q

D

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

Q

L

S
|
S

V

L

V

F

D

K

A

-

D

-

S

N

G

G

R

V

N

N

L

A

F

F

-

F

-

D

A

E

N

N

P

A

D

D

G

L

T

Q

D

L

S

S

P

E

L

T

F

A

L

K

S

H

H

F

I

I

A

A

G

G

L

I

Q

V

K

K

Y

H

L

A

P

T

Y

S

A

F

M

T

P

A

L

V

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

P

N

G

S

Y

D
x
E

A

A

P

P

I

C

N

Y

V

V

H

A

W

W

G

S

R

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

I

P

P

V

A

S
|
S

P

R

P

G

D

I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

N

G

K

L

L

E

E

P

A

N

P

D

A

P

P

I

I

K

D

G

R

S

N

A

I

Y

Y

R

V

L

M

A

S

-

K

-

E

-

R

-

M

-

E

-

N

-

G

-

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L

L

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P

R

A

H

T

Q

L

S

A

E

E

A

A

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K

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K

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A

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A

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K

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E

A

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active site: D52 (≠ L48), E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), H244 (= H257), R315 (= R328), E332 (= E345), R334 (= R347)
binding adenosine-5'-diphosphate: K43 (≠ R39), M50 (≠ L46), F198 (= F211), Y200 (≠ H213), N246 (≠ H259), S248 (= S261), S324 (= S337), S328 (≠ A341), R330 (= R343)
binding glutamic acid: E133 (= E138), E188 (= E201), V189 (≠ A202), N239 (≠ P252), G240 (= G253), G242 (≠ A255), E303 (≠ D316)
binding magnesium ion: E131 (= E136), E188 (= E201), E195 (= E208), H244 (= H257), E332 (= E345)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
29% identity, 99% coverage: 4:444/447 of query aligns to 8:440/443 of 4lniA

query
sites
4lniA

M

I

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x
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-

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-

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x
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E

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A

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E

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x
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S

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R

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E

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A

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E

A

A

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K

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L

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A

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A

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E

A

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W

E

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active site: D52 (≠ L48), E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), H244 (= H257), R315 (= R328), E332 (= E345), R334 (= R347)
binding adenosine-5'-diphosphate: E131 (= E136), E183 (≠ D196), D197 (≠ N210), Y200 (≠ H213), N246 (≠ H259), S248 (= S261), R320 (= R333), R330 (= R343)
binding magnesium ion: E131 (= E136), E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), E195 (= E208), H244 (= H257), E332 (= E345)
binding l-methionine-s-sulfoximine phosphate: E133 (= E138), E188 (= E201), H244 (= H257), R297 (= R310), E303 (≠ D316), R315 (= R328), R334 (= R347)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
29% identity, 99% coverage: 4:444/447 of query aligns to 9:441/444 of P12425

query
sites
P12425

M

I

E

E

D

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F

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A

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N

R

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P

G

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A

S

I

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-

V

-

Q

-

T

-

V

-

T

-

G

-

E

-

F

-

P

-

D

-

E

E

D
x
G

I

F

T

V

S
x
R

D

I

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

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D

D

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L

R

N

T

T

I

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I

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W

Y

T

T

A

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A

A

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E

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E

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E

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G

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H

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A

-

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-

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N

G

G

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x
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|
P

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N

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E

N

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R

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A

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A

A

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N

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A

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M

A

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-

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-

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-

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-

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-

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-

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-

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A

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A

A

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K

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A

K

K

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E

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W

K

D

A

M

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F

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M

G59 (≠ D66) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ S69) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E136) binding Mg(2+)
E134 (= E138) binding Mg(2+)
E189 (= E201) binding Mg(2+)
V190 (≠ A202) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E208) binding Mg(2+)
G241 (= G253) binding L-glutamate
H245 (= H257) binding Mg(2+)
G302 (≠ N314) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ D316) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P318) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E345) binding Mg(2+)
E424 (= E427) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s0rD Structure of gs-tnra complex (see paper)
29% identity, 99% coverage: 4:444/447 of query aligns to 12:444/447 of 4s0rD

query
sites
4s0rD

M

I

E

E

D

K

F

L

I

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K

K

K

E

N

E

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K

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P

A

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E

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K

Q

F

L

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G

R

K

I

A

L

L

R

D

D

N

R

K

G

V

L

M

R

F

L
x
D

P

G

E

S

A

S

I

I

F

-

V

-

Q

-

T

-

V

-

T

-

G

-

E

-

F

-

P

-

D

-

E

E

D

G

I
x
F

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x
V

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x
R

D
x
I

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

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Y

P

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D

D

E

L

R

N

T

T

I

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I

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W

Y

T

T

A

E

E

P

K

-

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-

K

T

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A

A

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I

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C

D

D

C

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Y

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A

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D

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P

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K

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Y

M

E

E

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L

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G

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K

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D

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-

N

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|
E

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|
E

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E

Y

-

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

K

N

G

G

G

R

Y

M

F

E
x
D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D

E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F

F

N

K

H

Y

G

A

D

G

A

A

L

V

E

R

L

S

A

C

D

D

Q

D

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G
|
G

E
x
V

P
x
N

G

G

S

S

A

G

M

M

H
|
H

I

C

H

N

Q

L

S

S

V

L

V

F

D

K

A

-

D

-

S

N

G

G

R

V

N

N

L

A

F

F

-

F

-

D

A

E

N

N

P

A

D

D

G

L

T

Q

D

L

S

S

P

E

L

T

F

A

L

K

S

H

H

F

I

I

A

A

G

G

L

I

Q

V

K

K

Y

H

L

A

P

T

Y

S

A

F

M

T

P

A

L

V

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

V

V

P

P

N
x
G

S
x
Y

D
x
E

A

A

P

P

I

C

N

Y

V

V

H

A

W

W

G

S

R

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

I

P

P

V

A

S

S

P

R

P

G

D

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

S

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

N

G

K

L

L

E

E

P

A

N

P

D

A

P

P

I

I

K

D

G

R

S

N

A

I

Y
|
Y

R

V

L

M

A

S

-

K

-

E

-

R

-

M

-

E

-

N

-

G

-

I

F

V

T

D

L

L

P

P

R

A

H

T

Q

L

S

A

E

E

A

A

L

L

S

E

K

E

F

F

N

K

A

S

C

N

K

E

P

V

L

M

K

V

E

K

I

A

L

L

G

G

E

E

R

H

F

L

I

F

D

E

A
x
H

V

F

D

I

C

E

V

A

K

K

Q

E

A
x
I

E

E

Y

W

K

D

A
x
M

Y

F

N

R

R

T

V

Q

I

V

S

H

S

P

W

W

E

E

R

R

E

E

N

Q

L

Y

L

M

active site: D56 (≠ L48), E135 (= E136), E137 (= E138), E192 (= E201), E199 (= E208), H248 (= H257), R319 (= R328), E336 (= E345), R338 (= R347)
binding glutamine: E137 (= E138), E192 (= E201), R301 (= R310), E307 (≠ D316)
binding magnesium ion: I66 (≠ D70), E135 (= E136), E135 (= E136), E199 (= E208), H248 (= H257), H248 (= H257), E336 (= E345), H419 (≠ A419)
binding : F63 (≠ I67), V64 (≠ T68), R65 (≠ S69), I66 (≠ D70), D161 (≠ E163), G241 (= G250), V242 (≠ E251), N243 (≠ P252), G305 (≠ N314), Y306 (≠ S315), Y376 (= Y385), I426 (≠ A426), M430 (≠ A430)

7tdpA Structure of paenibacillus polymyxa gs bound to met-sox-p-adp (transition state complex) to 1.98 angstom (see paper)
33% identity, 84% coverage: 71:444/447 of query aligns to 62:436/439 of 7tdpA

query
sites
7tdpA

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

Y

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

V

V

F

P

P

W

W

Y

-

T

S

E

D

P

R

T

V

A

A

Q

R

V

L

I

I

T

C

D

D

C

I

V

Y

Y

K

A

P

D

D

G

G

R

S

P

P

V

F

D

A

V

G

S

D

P

P

R

R

H

G

V

I

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

A

E

E

R

L

G

G

W

Y

K

T

P

S

L

M

-
x
N

V

V

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

T

N

D

K

E

D

K

P

G

D

D

Y

-

P

P

L

T

V

T

P

E

P

L

V

N

G

D

R

Q

N

G

G

G

R

Y

M

F

E

D

S

L

G

A

R

P

Q

M

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

E

F

-

D

-

P

-

I

-

F

-

E

-

A

-

V

I

Y

V

D

L

F

K

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D
x
E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N
x
D

F

F

N

K

H
x
Y

G

A

D

D

A

A

L

V

E

K

L

A

A

A

D

D

Q

Q

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

Q

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H
x
N

Q

Q

S
|
S

V

L

V

F

D

K

A

-

D

-

S

D

G

N

R

E

N

N

L

V

F

F

A

Y

N

-

P

-

D

D

G

E

T

T

D

D

S

E

P

-

L

L

F

G

L

L

S

S

-

Q

-

T

-

A

-

R

-

H

H

Y

I

M

A

A

G

G

L

I

Q

L

K

K

Y

H

L

A

P

R

Y

A

A

M

M

A

P

A

L

I

L

T

A

N

P

P

N

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

V

V

P

P

N

G

S

Y

D
x
E

A

A

P

P

I

C

N

Y

V

V

H

A

W

W

G

S

R

A

D

S

N

N

R
|
R

T

S

T

P

G

M

L

I

R
|
R

V

I

P

P

V

A

S

S

P

R

P

G

D

L

A

S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

V

N

A

P

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

L

A

A

I

L

A

A

A

V

S

M

L

L

A

R

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

R

G

Q

L

M

E

A

P

L

N

P

D

A

P

P

I

I

K

D

G

R

S

N

A

I

Y

Y

R

V

L

M

A

S

F

E

-

E

-

R

-

I

-

E

-

G

-

I

-

P

T

S

L

L

P

P

R

A

H

D

Q

L

S

K

E

E

A

A

L

L

S

S

K

E

F

L

N

I

A

R

C

S

K

E

P

V

L

I

K

S

E

D

I

A

L

L

G

G

E

D

R

H

F

A

I

L

D

A

A

Y

V

F

D

Y

C

E

V

L

K

K

Q

E

A

I

E

E

Y

W

K

D

A

M

Y

Y

N

R

R

T

V

Q

I

V

S

H

S

Q

W

W

E

E

R

R

E

D

N

Q

L

Y

L

L

binding adenosine-5'-diphosphate: N123 (vs. gap), G125 (≠ A134), E127 (= E136), E179 (≠ D196), D193 (≠ N210), Y196 (≠ H213), N242 (≠ H259), S244 (= S261), R316 (= R333), R326 (= R343)
binding magnesium ion: E127 (= E136), E127 (= E136), E129 (= E138), E184 (= E201), E191 (= E208), E191 (= E208), H240 (= H257), E328 (= E345)
binding l-methionine-s-sulfoximine phosphate: E127 (= E136), E129 (= E138), E184 (= E201), E191 (= E208), G236 (= G253), H240 (= H257), R293 (= R310), E299 (≠ D316), R311 (= R328), R330 (= R347)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
32% identity, 83% coverage: 73:444/447 of query aligns to 65:444/446 of A0R083

query
sites
A0R083

S

S

D

D

I

T

Y

V

M

A

I

R

P

P

D

D

E

P

R

S

T

T

I

F

R

Q

F

V

V

L

P

P

W

W

Y

K

T

T

E

S

P

D

-

G

-

N

-

H

-

Y

T

S

A

A

Q

R

V

M

I

F

T

C

D

D

C

I

V

T

Y

M

A

P

D

D

G

G

R

S

P

P

V

S

D

W

V

A

S

D

P

S

R

R

H

H

V

V

L

L

K

R

R

R

V

Q

L

L

S

A

L

K

Y

A

E

S

E

D

R

L

G

G

W

F

K

T

P

C

L

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

L

L

V

L

Q

K

V

P

N

G

K

P

D

N

P

D

D

G

Y

T

P

P

L

-

V

-

P

P

P

E

V

P

G

A

R

D

N

N

G

G

R

-

M

-

E

-

S

-

G

-

R

-

Q

-

A

G

F

Y

G

F

I

D

D

Q

A

A

V

V

N

H

E

D

F

A

D

A

P

P

I

N

F

F

E

R

-

R

A

H

V

A

Y

I

D

E

F

A

C

L

E

E

K

Q

Q

M

D

G

I

I

D

S

I

V

D

E

T

F

L

S

T

H

H

H

E

E

A

G

G

A

A

P

A

G

Q

Q

I

Q

E

E

I

I

N

D

F

L

N

R

H

Y

G

A

D

D

A

A

L

L

E

S

L

M

A

A

D

D

Q

N

A

V

F

M

L

T

F

F

K

R

R

Y

T

L

A

V

R

K

E

E

A

V

A

A

I

L

R

A

H

D

Q

G

V

V

Y

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

M

F

Q

A

G

E

E

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

V

L

V

F

D

E

A

G

D

D

S

T

G

-

R

-

N

N

L

A

F

F

A

H

N

S

P

P

D

-

G

-

T

-

D

D

S

D

P

P

L

L

F

Q

L

L

-

S

-

D

-

V

-

A

-

K

S

S

H

F

I

I

A

A

G

G

L

I

Q

L

K

E

Y

H

L

A

P

N

Y

E

A

I

M

S

P

A

L

V

L

T

A

N

P

Q

N

W

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

H

N

G

S

G

D

E

A

A

P

P

I

T

N

A

V

A

H

S

W

W

G

G

R

A

D

A

N

N

R

R

T

S

T

A

G

L

L

V

R

R

V

V

P

P

V

M

S

Y

P

T

P

P

-

H

-

K

-

V

D

S

A

S

R

R

V

V

E

E

N

V

R

R

V

S

A

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

L

L

A

T

I

F

A

A

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

R

G

G

M

V

T

E

Q
x
K

G

G

-

Y

-

V

L

L

E

G

P

P

-

Q

-

A

-

E

-

D

-

N

-

V

-

W

-

S

-

L

-

T

N

Q

D

E

P

E

I

R

K

R

G

A

S

M

A

G

Y

Y

R

R

L

-

A

-

F

-

T

E

L

L

P

P

R

T

H

S

Q

L

S

G

E

N

A

A

L

L

S

E

K

S

F

M

N

E

A

N

C

S

K

E

P

L

L

V

K

A

E

E

I

A

L

L

G

G

E

E

R

H

F

V

I

F

D

D

A

Y

V

F

D

L

C

R

V

N

K

K

Q

R

A

S

E

E

Y

W

K

E

A

N

Y

Y

N

R

R

S

V

H

I

V

S

T

S

P

W

Y

E

E

R

L

E

K

N

N

L

Y

L

L

K363 (≠ Q372) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 88% coverage: 50:444/447 of query aligns to 42:444/446 of P9WN37

query
sites
P9WN37

E

E

A

G

I

A

F

F

V

E

Q

E

T

G

V

I

T

G

G

F

E

D

F

G

P

S

D

S

D

I

E

E

D

G

I

F

T

A

S

R

D

V

E

S

N

E

S

S

D

D

I

T

Y

V

M

A

I

H

P

P

D

D

E

P

R

S

T

T

I

F

R

Q

F

V

V

L

P

P

W

W

Y

A

T

T

E

S

P

S

-

G

-

H

T

S

A

A

Q

R

V

M

I

F

T

C

D

D

C

I

V

T

Y

M

A

P

D

D

G

G

R

S

P

P

V

S

D

W

V

A

S

D

P

P

R

R

H

H

V

V

L

L

K

R

R

R

V

Q

L

L

S

T

L

K

Y

A

E

G

E

E

R

L

G

G

W

F

K

S

P

C

L

Y

V

V

A

H

P

P

E

E

L

I

E

E

F

F

L

L

V

L

Q

K

V

-

N

-

K

P

D

G

P

P

D

E

Y

D

P

G

L

S

V

V

P

P

-

V

P

P

V

V

G

D

R

N

N

A

G

G

R

Y

M

F

E

D

S

-

G

-

R

-

Q

Q

A

A

F

V

G

H

I

D

D

S

A

A

V

L

N

N

E

-

F

-

D

-

P

-

I

F

F

R

E

R

A

H

V

A

Y

I

D

D

F

A

C

L

E

E

K

F

Q

M

D

G

I

I

D

S

I

V

D

E

T

F

L

S

T

H

H

H

E

E

A

G

G

A

A

P

A

G

Q

Q

I

Q

E

E

I

I

N

D

F

L

N

R

H

F

G

A

D

D

A

A

L

L

E

S

L

M

A

A

D

D

Q

N

A

V

F

M

L

T

F

F

K

R

R

Y

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A

I

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K

E

E

A

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A

A

I

L

R

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H

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Q

G

V

A

Y

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A

A

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S

F

F

M

M

A

P

K

K

P

P

M

F

Q

G

G

Q

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H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

Q

M

S

S

V

L

V

F

D

E

A

G

D

D

S

V

G

-

R

-

N

N

L

A

F

F

A

H

N

S

P

-

D

-

G

-

T

A

D

D

S

D

P

P

L

L

F

Q

L

L

S

S

H

E

-

V

-

G

-

K

-

S

-

F

I

I

A

A

G

G

L

I

Q

L

K

E

Y

H

L

A

P

C

Y

E

A

I

M

S

P

A

L

V

L

T

A

N

P

Q

N

W

V

V

N

N

S

S

Y

Y

R

K

R

R

L

L

V

V

P

Q

N

G

S

G

D

E

A

A

P

P

I

T

N

A

V

A

H

S

W

W

G

G

R

A

D

A

N

N

R

R

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S

T

A

G

L

L

V

R

R

V

V

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P

V

M

S

Y

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P

P

-

H

-

K

-

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D

S

A

S

R

R

V

V

E

E

N

V

R

R

V

S

A

P

G

D

A

S

D

A

A

C

N

N

P

P

Y

Y

L

L

A

T

I

F

A

A

A

V

S

L

L

L

A

A

C

A

G

G

Y

L

I

R

G

G

M

V

T

E

Q
x
K

G

G

-

Y

-

V

-

L

-

G

-

P

-

Q

-

A

-

E

-

D

-

N

-

V

-

W

-

D

L

L

E

T

P

P

N

E

D

E

P

R

I

-

K

R

G

A

S

M

A

G

Y

Y

R

R

L

-

A

-

F

-

T

E

L

L

P

P

R

S

H

S

Q

L

S

D

E

S

A

A

L

L

S

R

K

A

F

M

N

E

A

A

C

S

K

E

P

L

L

V

K

A

E

E

I

A

L

L

G

G

E

E

R

H

F

V

I

F

D

D

A

F

V

F

D

L

C

R

V

N

K

K

Q

R

A

T

E

E

Y

W

K

A

A

N

Y

Y

N

R

R

S

V

H

I

V

S

T

S

P

W

Y

E

E

R

L

E

R

N

T

L

Y

L

L

K363 (≠ Q372) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
32% identity, 81% coverage: 77:439/447 of query aligns to 56:390/396 of 5dm3C

query
sites
5dm3C

M

M

I

K

P

P

D

D

E

L

R

A

T

T

I

L

R

R

F

C

V

V

P

P

W

W

Y

L

T

-

E

E

P

G

T

T

A

A

Q

M

V

V

I

L

T

C

D

D

C

L

V

L

-

D

Y

H

A

R

D

T

G

H

R

A

P

E

V

V

D

P

V

H

S

A

P

P

R

R

H

A

V

I

L

L

K

K

R

R

V

Q

L

L

S

A

L

R

Y

L

E

E

E

A

R

M

G

G

W

L

K

E

P

A

L

I

V

M

A

A

P

T

E
|
E

L

L

E
|
E

F

F

L

L

V

F

Q

E

V

K

N

S

K

L

D

D

P

E

D

I

Y

-

P

-

L

-

V

-

P

-

V

-

G

-

R

R

N

K

G

G

R

R

M

F

E

R

S

T

G

T

R

K

Q

E

A

-

F

-

G

-

I

-

D

-

A

-

V

-

N

-

E

-

F

-

D

E

P

H

I

V

F

L

E

R

A

P

V

L

Y

R

D

N

F

H

C

L

E

H

K

A

Q

A

D

G

I

I

D

P

I

V

D

E

T

G

L

T

T

K

H

G

E
|
E

A

A

G

G

A

A

A

G

Q

Q

I

E

E
|
E

I

L

N

N

F

I

N
x
R

H
x
C

G

A

D

K

A

A

L

L

E

D

L

T

A

A

D

D

Q

Y

A

H

F

T

L

I

F

A

K

K

R

H

T

A

A

T

R

K

E

E

A

I

A

A

I

W

R

Q

H

Q

Q

G

V

R

Y

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

M

H

Q

H

G

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

I

I

H
|
H

Q

Q

S
|
S

V

L

V

W

D

K

A

-

D

-

S

-

G

-

R

Q

N

G

L

L

F

P

A

A

N

F

P

H

D

D

G

E

T

R

D

D

-

A

-

L

-

G

-

M

S

S

P

A

L

L

F

M

L

K

S

H

H

Y

I

L

A

A

G

G

L

L

Q

L

K

K

Y

Y

L

A

P

P

Y

D

A

Y

M

T

P

Y

L

F

L

L

A

A

P

P

N

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

L

F

V

-

P

-

N

-

S

Q

D

F

A

A

P

P

I

T

N

R

V

T

H

V

W

W

G

S

R

V

D

D

N

N

R
|
R

T

T

T

A

G

G

L

F

R

R

V

L

P

C

V

A

S

E

P

G

P

T

D

R

A

A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

V

I

A

G

G

G

A

S

D

D

A

L

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

A

G

S

Q

L

L

A

A

C

A

G

G

Y

I

I

K

G

G

M

I

T

E

Q

E

G

C

L

L

E

A

P

L

N

P

D

P

P

P

I

A

K

S

G

G

S

-

A

-

Y

-

R

-

L

-

A

-

F

-

T

L

L

I

P

P

R

Q

H

N

Q

L

S

R

E

D

A

A

L

M

S

E

K

A

F

L

N

R

A

G

C

S

K

T

P

M

L

L

K

R

E

E

I

A

L

M

G

G

E

E

R

D

F

V

I

V

D

D

A

H

V

Y

D

V

C

R

V

A

K

A

Q

E

A

V

E

E

Y

L

K

E

A

D

Y

F

N

Q

R

R

V

V

I

V

S

S

S

D

W

Y

E

E

active site: E115 (= E136), E117 (= E138), E162 (= E201), E169 (= E208), H218 (= H257), R286 (= R328), E303 (= E345), R305 (= R347)
binding adenosine-5'-diphosphate: R173 (≠ N212), C174 (≠ H213), H220 (= H259), S222 (= S261), R301 (= R343)

7tenA Glutamine synthetase (see paper)
30% identity, 84% coverage: 71:444/447 of query aligns to 62:439/442 of 7tenA

query
sites
7tenA

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

F

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

V

V

F

P

P

W

W

Y

T

T

A

E

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

T

C

D

D

C

I

V

Y

Y

N

A

P

D

D

G

M

R

T

P

P

V

F

D

A

V

G

S

D

P

P

R

R

H

A

V

N

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

M

E

E

R

L

G

G

W

F

K

T

P

E

L

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

L

N

D

K

E

D

N

P

-

D

R

Y

R

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

S

N

G

G

G

R

Y

M

F

E

D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D
x
E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N
x
D

F
|
F

N
x
K

H
x
Y

G

E

D

D

A

A

L

I

E

T

L

A

A

C

D

D

Q

S

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q

M

S
|
S

V

L

V

F

D

N

A

-

D

E

S

K

G

G

R

N

N

A

L

F

F

F

A

D

N

E

P

S

D

G

G

E

T

L

D

E

S

L

P

S

L

Q

F

T

L

A

S

Y

H

H

-

F

I

L

A

A

G

G

L

M

Q

L

K

K

Y

H

L

A

P

R

Y

G

A

Y

M

T

P

A

L

V

L

T

A

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

V

V

P

P

N

G

S

Y

D
x
E

A

A

P

P

I

C

N

Y

V

I

H

A

W

W

G

S

R

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

V

R
|
R

V

V

P

P

V

S

S

S

P

R

P

G

D

L

A
x
S

R

T

R
|
R

V

L

E

E

N

L

R
|
R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

A

V

S

L

L

L

A

K

C

A

G

G

Y

L

I

S

G

G

M

I

T

K

Q

D

G

E

L

L

E

T

P

P

N

P

D

A

P

P

I

V

K

D

G

R

S

N

A

I

Y

Y

R

G

L

M

-

N

-

E

-

R

-

E

-

A

-

T

-

G

A

I

F

Y

T

D

L

L

P

P

R

E

H

S

Q

L

S

G

E

H

A

A

L

L

S

I

K

E

F

L

N

E

A

K

C

N

K

E

P

I

L

I

K

K

E

D

I

G

L

L

G

G

E

E

R

H

F

I

I

F

D

E

A

H

V

F

D

I

C

E

V

A

K

K

Q

T

A

I

E

E

Y

C

K

D

A

M

Y

F

N

R

R

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

E

E

N

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A134), E130 (= E136), E182 (≠ D196), D196 (≠ N210), F197 (= F211), K198 (≠ N212), Y199 (≠ H213), N245 (≠ H259), S247 (= S261), R319 (= R333), S327 (≠ A341), R329 (= R343)
binding l-methionine-s-sulfoximine phosphate: E130 (= E136), E132 (= E138), E187 (= E201), E194 (= E208), N238 (≠ P252), G239 (= G253), H243 (= H257), R296 (= R310), E302 (≠ D316), R314 (= R328), R333 (= R347)

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
30% identity, 84% coverage: 71:444/447 of query aligns to 63:440/443 of 7tf9S

query
sites
7tf9S

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

F

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

V

V

F

P

P

W

W

Y

T

T

A

E

E

P

K

-

G

-

K

T

V

A

A

Q

R

V

M

I

I

T

C

D

D

C

I

V

Y

Y

N

A

P

D

D

G

M

R

T

P

P

V

F

D

A

V

G

S

D

P

P

R

R

H

A

V

N

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

M

E

E

R

L

G

G

W

F

K

T

P

E

L

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

L

N

D

K

E

D

N

P

-

D

R

Y

R

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

S

N

G

G

G

R
x
Y

M

F

E

D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

E

Q

M

D

G

I

F

D

E

I

I

D

E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F

F

N

K

H

Y

G

E

D

D

A

A

L

I

E

T

L

A

A

C

D

D

Q

S

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

G

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

F

H

N

Q

M

S

S

V

L

V

F

D

N

A

-

D

E

S

K

G

G

R

N

N

A

L

F

F

F

A

D

N

E

P

S

D

G

G

E

T

L

D

E

S

L

P

S

L

Q

F

T

L

A

S

Y

H

H

-

F

I

L

A

A

G

G

L

M

Q

L

K

K

Y

H

L

A

P

R

Y

G

A

Y

M

T

P

A

L

V

L

T

A

N

P

P

N

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

L

L

V

V

P

P

N

G

S

Y

D
x
E

A

A

P

P

I

C

N

Y

V

I

H

A

W

W

G

S

R

G

D

K

N

N

R

R

T

S

T

P

G

L

L

V

R

R

V

V

P

P

V

S

S

S

P

R

P

G

D

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

V

S

A

V

G

D

A

P

D

S

A

A

N

N

P

P

Y

Y

L

L

A

A

I

M

A

A

A

V

S

L

L

L

A

K

C

A

G

G

Y

L

I

S

G

G

M

I

T

K

Q

D

G

E

L

L

E

T

P

P

N

P

D

A

P

P

I

V

K

D

G

R

S

N

A

I

Y

Y

R

G

L

M

-

N

-

E

-

R

-

E

-

A

-

T

-

G

A

I

F

Y

T

D

L

L

P

P

R

E

H

S

Q

L

S

G

E

H

A

A

L

L

S

I

K

E

F

L

N

E

A

K

C

N

K

E

P

I

L

I

K

K

E

D

I

G

L

L

G

G

E

E

R

H

F

I

I

F

D

E

A

H

V

F

D

I

C
x
E

V

A

K

K

Q

T

A
x
I

E

E

Y

C

K

D

A
x
M

Y

F

N

R

R

T

V

A

I

V

S

H

S

P

W

W

E

E

R

R

E

E

N

Q

L

Y

L

L

binding glutamine: E133 (= E138), Y155 (≠ R161), E188 (= E201), G240 (= G253), G242 (≠ A255), R297 (= R310), E303 (≠ D316)
binding magnesium ion: E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), H244 (= H257), E332 (= E345)
binding : E418 (≠ C422), I422 (≠ A426), M426 (≠ A430)

Sites not aligning to the query:

binding : 59, 60

7tdvC Glutamine synthetase (see paper)
27% identity, 98% coverage: 7:444/447 of query aligns to 11:440/443 of 7tdvC

query
sites
7tdvC

F

F

I

V

K

E

N

E

R

N

I

V

T

R

E

Y

V

L

E

R

C

L

L

Q

V

F

P

T

D

D

M

I

S

L

G

G

I

T

A

I

R

K

G

N

K

V

I

E

V

V

P

P

A

V

E

S

K

Q

F

L

L

E

R

K

I

V

L

L

R

D

D

N

R

E

G

M

L

M

R

F

L

D

P

G

E

S

A

S

I

I

F

-

V

-

Q

-

T

-

V

-

T

-

G

-

E

-

F

-

P

-

D

-

E

E

D

G

I

F

T

V

S

R

D

I

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

H

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

I

V

F

P

P

W

W

Y

T

T

A

E

G

-

Q

-

G

P

K

T

V

A

A

Q

R

V

L

I

I

T

C

D

D

C

V

V

Y

Y

K

A

T

D

D

G

G

R

T

P

P

V

F

D

E

V

G

S

D

P

P

R

R

H

A

V

N

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

M

E

E

E

D

R

L

G

G

W

F

K

T

P

D

L

F

-

N

V

L

A
x
G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

L

N

D

K

E

D

K

P

G

D

E

Y

-

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

D

N

G

G

G

R

Y

M

F

E

D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

D

Q

M

D

G

I

F

D

D

I

I

D
x
E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N
x
D

F
|
F

N
x
K

H
x
Y

G

A

D

D

A

A

L

V

E

T

L

A

A

C

D

D

Q

N

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

N

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H
x
N

Q
x
V

S
|
S

V

L

V

F

D

K

A

G

D

K

S

E

G

N

R

A

N

F

L

F

F

D

A

P

N

N

P

T

D

E

G

M

T

G

D

L

S

T

P

E

L

T

F

A

L

Y

S

Q

H

F

I

T

A

A

G

G

L

V

Q

L

K

K

Y

N

L

A

P

R

Y

G

A

F

M

T

P

A

L

V

L

C

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

V

V

P

P

N

G

S

Y

D
x
E

A

A

P

P

I

C

N

Y

V

I

H

A

W

W

G

S

R

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

I

R
|
R

V

V

P

P

V

S

S

S

P

R

P

G

D

L

A
x
S

R

T

R
|
R

V

I

E
|
E

N

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

I

L

A

A

S

I

L

L

A

E

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

N

G

K

L

L

E

K

P

V

N

P

D

E

P

P

I

V

K

N

G

Q

S

N

A

I

Y

Y

R

E

L

M

A

N

F

R

T

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

H

T

Q

L

S

Y

E

T

A

A

L

L

S

K

K

A

F

M

N

R

A

E

C

N

K

E

P

V

L

I

K

K

E

K

I

A

L

L

G

G

E

N

R

H

F

I

I

Y

D

N

A

Q

V

F

D

I

C

N

V

S

K

K

Q

S

A

I

E

E

Y

W

K

D

A

Y

Y

Y

N

R

R

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

E

D

N

Q

L

Y

L

M

binding adenosine-5'-diphosphate: G129 (≠ A134), E131 (= E136), E183 (≠ D196), D197 (≠ N210), F198 (= F211), K199 (≠ N212), Y200 (≠ H213), N246 (≠ H259), V247 (≠ Q260), S248 (= S261), R320 (= R333), S328 (≠ A341), R330 (= R343)
binding magnesium ion: E131 (= E136), E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), E195 (= E208), H244 (= H257), E332 (= E345)
binding l-methionine-s-sulfoximine phosphate: E131 (= E136), E133 (= E138), E188 (= E201), E195 (= E208), G240 (= G253), H244 (= H257), R297 (= R310), E303 (≠ D316), R315 (= R328)

7tf6A Glutamine synthetase (see paper)
30% identity, 84% coverage: 71:444/447 of query aligns to 62:435/438 of 7tf6A

query
sites
7tf6A

E

E

N

E

S

S

D

D

I

M

Y

Y

M

L

I

H

P

P

D

D

E

L

R

D

T

T

I

W

R

V

F

I

V

F

P

P

W

W

Y

-

T

-

E

G

P

K

T

V

A

A

Q

R

V

L

I

I

T

C

D

D

C

V

V

Y

Y

K

A

T

D

D

G

G

R

T

P

P

V

F

D

E

V

G

S

D

P

P

R

R

H

A

V

N

L

L

K

K

R

R

V

V

L

L

S

K

L

E

Y

M

E

E

E

D

R

L

G

G

W

F

K

T

P

D

L

F

-

N

V

L

A

G

P

P

E
|
E

L

P

E
|
E

F

F

L

L

V

F

Q

K

V

L

N

D

K

E

D

K

P

G

D

E

Y

-

P

P

L

T

V

L

P

E

P

L

V

N

G

D

R

D

N

G

G

G

R

Y

M

F

E

D

S

L

G

A

R

P

Q

T

A

D

F

L

G

G

I

E

D

N

A

C

V

R

N

R

E

-

F

-

D

D

P

I

I

V

F

L

E

E

A

-

V

-

Y

-

D

-

F

-

C

L

E

E

K

D

Q

M

D

G

I

F

D

D

I

I

D

E

T

A

L

S

T

H

H

H

E
|
E

A

V

G

A

A

P

A

G

Q

Q

I

H

E
|
E

I

I

N

D

F

F

N

K

H

Y

G

A

D

D

A

A

L

V

E

T

L

A

A

C

D

D

Q

N

A

I

F

Q

L

T

F

F

K

K

R

L

T

V

A

V

R

K

E

T

A

I

A

A

I

R

R

K

H

H

Q

N

V

L

Y

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

M

L

Q

F

G
|
G

E

V

P
x
N

G
|
G

S

S

A

G

M

M

H
|
H

I

F

H

N

Q

V

S

S

V

L

V

F

D

K

A

G

D

K

S

E

G

N

R

A

N

F

L

F

F

D

A

P

N

N

P

T

D

E

G

M

T

G

D

L

S

T

P

E

L

T

F

A

L

Y

S

Q

H

F

I

T

A

A

G

G

L

V

Q

L

K

K

Y

N

L

A

P

R

Y

G

A

F

M

T

P

A

L

V

L

C

A

N

P

P

N

L

V

V

N

N

S

S

Y

Y

R

K

R
|
R

L

L

V

V

P

P

N
x
G

S
x
Y

D
x
E

A

A

P

P

I

C

N

Y

V

I

H

A

W

W

G

S

R

G

D

K

N

N

R
|
R

T

S

T

P

G

L

L

I

R

R

V

V

P

P

V

S

S

S

P

R

P

G

D

L

A

S

R

T

R

R

V

I

E
|
E

N

V

R

R

V

S

A

V

G

D

A

P

D

A

A

A

N

N

P

P

Y

Y

L

M

A

A

I

L

A

A

S

I

L

L

A

E

C

A

G

G

Y

L

I

D

G

G

M

I

T

K

Q

N

G

K

L

L

E

K

P

V

N

P

D

E

P

P

I

V

K

N

G

Q

S

N

A

I

Y
|
Y

R

E

L

M

A

N

F

R

T

E

-

E

-

R

-

E

-

A

-

V

-

G

-

I

-

Q

-

D

L

L

P

P

R

S

H

T

Q

L

S

Y

E

T

A

A

L

L

S

K

K

A

F

M

N

R

A

E

C

N

K

E

P

V

L

I

K

K

E

K

I

A

L

L

G

G

E

N

R

H

F

I

I

Y

D

N

A

Q

V

F

D

I

C

N

V

S

K

K

Q

S

A

I

E

E

Y

W

K

D

A
x
Y

Y

Y

N

R

R

T

V

Q

I

V

S

S

S

E

W

W

E

E

R

R

E

D

N
x
Q

L

Y

L

M

binding glutamine: E128 (= E138), E183 (= E201), G235 (= G253), H239 (= H257), R292 (= R310), E298 (≠ D316)
binding magnesium ion: E126 (= E136), E128 (= E138), E183 (= E201), E190 (= E208), H239 (= H257), E327 (= E345)
binding : G232 (= G250), N234 (≠ P252), G296 (≠ N314), Y297 (≠ S315), R310 (= R328), Y367 (= Y385), Y421 (≠ A430), Q433 (≠ N442)

Sites not aligning to the query:

binding : 58, 60, 437

Query Sequence

>WP_012977188.1 NCBI__GCF_000010725.1:WP_012977188.1
MGFMEDFIKKNRITEVECLVPDMSGIARGKIVPAEKFLRILRDRGLRLPEAIFVQTVTGE
FPDDEDITSDENSDIYMIPDERTIRFVPWYTEPTAQVITDCVYADGRPVDVSPRHVLKRV
LSLYEERGWKPLVAPELEFFLVQVNKDPDYPLVPPVGRNGRMESGRQAFGIDAVNEFDPI
FEAVYDFCEKQDIDIDTLTHEAGAAQIEINFNHGDALELADQAFLFKRTAREAAIRHQVY
ATFMAKPMQGEPGSAMHIHQSVVDADSGRNLFANPDGTDSPLFLSHIAGLQKYLPYAMPL
LAPNVNSYRRLVPNSDAPINVHWGRDNRTTGLRVPVSPPDARRVENRVAGADANPYLAIA
ASLACGYIGMTQGLEPNDPIKGSAYRLAFTLPRHQSEALSKFNACKPLKEILGERFIDAV
DCVKQAEYKAYNRVISSWERENLLLNV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory