SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012991705.1 NCBI__GCF_000025605.1:WP_012991705.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 10 hits to proteins with known functional sites (download)

6n2oC 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
47% identity, 95% coverage: 3:578/606 of query aligns to 4:567/572 of 6n2oC

query
sites
6n2oC

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binding succinyl-coenzyme a: G14 (= G13), G16 (= G15), I17 (≠ V16), I18 (= I17), S19 (= S18), F41 (= F40), A43 (= A42), E44 (= E43), I45 (= I44), R128 (≠ I125), N131 (= N128), F157 (= F154), N168 (= N165), T226 (= T224), R302 (= R300)
binding thiamine diphosphate: P224 (= P222), I225 (= I223), E252 (= E250)

6n2oA 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
47% identity, 95% coverage: 3:578/606 of query aligns to 4:567/572 of 6n2oA

query
sites
6n2oA

D

D

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F

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C

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F

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G

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binding 2-oxoglutaric acid: T226 (= T224), R302 (= R300)
binding coenzyme a: G14 (= G13), G16 (= G15), S19 (= S18), N131 (= N128), K156 (= K153)
binding thiamine diphosphate: Y223 (= Y221), P224 (= P222), I225 (= I223), E252 (= E250)

6n2nA Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
47% identity, 95% coverage: 3:578/606 of query aligns to 4:567/572 of 6n2nA

query
sites
6n2nA

D

D

L

L

T

I

V

I

K

R

I

V

G

A

G

G

E

E

G

G

G

G

E

E

G

G

V

I

I

I

S

S

A

S

G

G

D

D

F

F

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A

E

A

A

A

C

C

A

A

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R

A

A

G

G

Y

L

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E

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V

Y

N

T

F

F

K

K

S

T

F

F

P

P

A

A

E

E

I

I

K

K

G

G

G

G

Y

Y

A

A

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S

Y

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Q

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R

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A

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S

D

S

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E

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L

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G

G

D

D

S

T

F

F

D

D

V

V

L

F

C

C

C

A

F

F

N

N

G

G

E

E

A

A

Y

Y

E

E

F

Q

N

N

K

K

K

D

H

K

L

I

V

K

G

P

G

G

T

T

V

A

M

F

V

V

Y

Y

D

D

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Y

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binding thiamine diphosphate: Y223 (= Y221), P224 (= P222), I225 (= I223), E252 (= E250)

P72578 2-oxoacid:ferredoxin oxidoreductase subunit alpha; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see 2 papers)
30% identity, 95% coverage: 8:584/606 of query aligns to 7:616/632 of P72578

query
sites
P72578

I

I

G

G

G

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D

Y253 (= Y221) mutation Y->A,W: Loss of oxidoreductase activity.; mutation to F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
YPITP 253:257 (= YPITP 221:225) YPITP motif
P254 (= P222) mutation to G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
I255 (= I223) mutation I->L,M,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
T256 (= T224) mutation T->A,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
P257 (= P225) mutation P->A,G,V: Loss of oxidoreductase activity.
R344 (= R300) mutation R->A,K: Loss of oxidoreductase activity.
T353 (= T309) Plays a key role in the broad substrate specificity; mutation T->I,V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.

Q96Y66 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
29% identity, 95% coverage: 8:584/606 of query aligns to 7:616/627 of Q96Y66

query
sites
Q96Y66

I

I

G

G

G

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A

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G

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S41 (≠ A42) mutation to A: Same oxidoreductase activity as the wild-type.
T349 (= T305) mutation to L: Same oxidoreductase activity as the wild-type.
D468 (≠ L434) mutation to A: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.

5b48A 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
29% identity, 95% coverage: 8:584/606 of query aligns to 7:568/576 of 5b48A

query
sites
5b48A

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binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: Y221 (= Y221), P222 (= P222), I223 (= I223), E262 (= E250), R312 (= R300)

Q96XT2 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
29% identity, 95% coverage: 8:583/606 of query aligns to 8:616/628 of Q96XT2

query
sites
Q96XT2

I

I

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A

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T257 (= T224) binding substrate
R344 (= R300) binding substrate

5b47A 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
29% identity, 95% coverage: 8:583/606 of query aligns to 7:615/627 of 5b47A

query
sites
5b47A

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