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Comparing WP_012991706.1 NCBI__GCF_000025605.1:WP_012991706.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 13 hits to proteins with known functional sites (download)
6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
48% identity, 99% coverage: 2:288/291 of query aligns to 11:291/291 of 6n2oD
- binding magnesium ion: D101 (= D92), N129 (= N120), I131 (≠ V122)
- binding succinyl-coenzyme a: I57 (= I48), R62 (= R53), L134 (= L125), K136 (= K127)
- binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (= C50), C208 (= C199), T210 (= T201), F211 (= F202)
- binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (= C50), S60 (= S51), H76 (= H67), G102 (= G93), D103 (= D94), N129 (= N120), I131 (≠ V122), G133 (= G124), L134 (= L125), T135 (= T126)
6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
48% identity, 99% coverage: 2:288/291 of query aligns to 11:291/291 of 6n2oB
- binding 2-oxoglutaric acid: R62 (= R53), L134 (= L125)
- binding coenzyme a: K136 (= K127), Y150 (≠ S141)
- binding magnesium ion: D101 (= D92), N129 (= N120), I131 (≠ V122)
- binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), C59 (= C50), C208 (= C199), T210 (= T201), F211 (= F202)
- binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (= C50), S60 (= S51), H76 (= H67), G102 (= G93), D103 (= D94), N129 (= N120), I131 (≠ V122), Y132 (= Y123), G133 (= G124), L134 (= L125), T135 (= T126)
6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
48% identity, 99% coverage: 2:288/291 of query aligns to 11:291/291 of 6n2nB
- binding magnesium ion: D101 (= D92), N129 (= N120), I131 (≠ V122)
- binding iron/sulfur cluster: W24 (= W15), C25 (= C16), C28 (= C19), H30 (≠ D21), C59 (= C50), C208 (= C199), T210 (= T201), F211 (= F202)
- binding thiamine diphosphate: I57 (= I48), G58 (= G49), C59 (= C50), S60 (= S51), H76 (= H67), G100 (= G91), D101 (= D92), G102 (= G93), D103 (= D94), N129 (= N120), I131 (≠ V122), Y132 (= Y123), G133 (= G124), L134 (= L125), T135 (= T126)
Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
44% identity, 89% coverage: 8:267/291 of query aligns to 4:269/304 of Q96XT4
- C12 (= C16) binding [4Fe-4S] cluster
- C15 (= C19) binding [4Fe-4S] cluster
- IGCS 44:47 (= IGCS 48:51) binding thiamine diphosphate
- C46 (= C50) binding [4Fe-4S] cluster
- D90 (= D92) binding Mg(2+)
- GD 91:92 (= GD 93:94) binding thiamine diphosphate
- N118 (= N120) binding Mg(2+)
- V120 (= V122) binding Mg(2+)
- GL 122:123 (= GL 124:125) binding thiamine diphosphate
- C197 (= C199) binding [4Fe-4S] cluster
5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
44% identity, 89% coverage: 8:267/291 of query aligns to 1:266/301 of 5b46B
- binding magnesium ion: D87 (= D92), N115 (= N120), V117 (= V122)
- binding iron/sulfur cluster: W8 (= W15), C9 (= C16), C12 (= C19), C43 (= C50), C194 (= C199), T196 (= T201), Y197 (≠ F202)
- binding thiamine diphosphate: I41 (= I48), G42 (= G49), C43 (= C50), S44 (= S51), H62 (= H67), G86 (= G91), G88 (= G93), D89 (= D94), N115 (= N120), V117 (= V122), Y118 (= Y123), G119 (= G124), L120 (= L125), T121 (= T126)
Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
43% identity, 87% coverage: 15:266/291 of query aligns to 11:268/305 of Q96Y68
- C12 (= C16) binding [4Fe-4S] cluster
- C15 (= C19) binding [4Fe-4S] cluster
- C46 (= C50) binding [4Fe-4S] cluster
- K49 (≠ R53) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
- D90 (= D92) binding Mg(2+)
- GD 91:92 (= GD 93:94) binding thiamine diphosphate
- N118 (= N120) binding Mg(2+)
- V120 (= V122) binding Mg(2+)
- GL 122:123 (= GL 124:125) binding thiamine diphosphate
- K125 (= K127) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
- K173 (≠ Q175) mutation to A: Same oxidoreductase activity as the wild-type.
- C197 (= C199) binding [4Fe-4S] cluster
P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
43% identity, 87% coverage: 15:266/291 of query aligns to 11:268/305 of P72579
- K49 (≠ R53) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
- L123 (= L125) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.
5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
45% identity, 87% coverage: 15:266/291 of query aligns to 7:250/286 of 5b48B
- binding magnesium ion: D84 (= D92), N112 (= N120), V114 (= V122)
- binding iron/sulfur cluster: C8 (= C16), C11 (= C19), C42 (= C50), C183 (= C199), T185 (= T201)
- binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I48), G41 (= G49), C42 (= C50), S43 (= S51), H59 (= H67), G85 (= G93), D86 (= D94), N112 (= N120), V114 (= V122), Y115 (= Y123), G116 (= G124), L117 (= L125)
5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
43% identity, 87% coverage: 15:266/291 of query aligns to 4:239/275 of 5b47B
- binding magnesium ion: D83 (= D92), N111 (= N120)
- binding iron/sulfur cluster: C5 (= C16), C8 (= C19), C39 (= C50), C179 (= C199)
- binding thiamine diphosphate: I37 (= I48), G38 (= G49), C39 (= C50), S40 (= S51), H58 (= H67), G84 (= G93), D85 (= D94), N111 (= N120), V113 (= V122), Y114 (= Y123), L116 (= L125)
9bt4C Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
29% identity, 68% coverage: 16:212/291 of query aligns to 13:222/291 of 9bt4C
- binding magnesium ion: D94 (= D92), N122 (= N120), A124 (≠ V122)
- binding iron/sulfur cluster: C13 (= C16), C16 (= C19), C17 (≠ G20), D18 (= D21), C41 (= C50), M126 (≠ G124), C206 (= C199), T208 (= T201)
- binding thiamine diphosphate: D18 (= D21), T39 (≠ I48), G40 (= G49), C41 (= C50), F63 (≠ H67), E64 (≠ G68), G93 (= G91), D94 (= D92), G95 (= G93), S96 (≠ D94), N122 (= N120), A124 (≠ V122), Y125 (= Y123), M126 (≠ G124), N127 (≠ L125), T128 (= T126)
5c4iC Structure of an oxalate oxidoreductase (see paper)
22% identity, 93% coverage: 16:285/291 of query aligns to 24:298/312 of 5c4iC
- active site: N143 (≠ L125)
- binding magnesium ion: D110 (= D92), N138 (= N120), S140 (≠ V122)
- binding iron/sulfur cluster: C24 (= C16), C27 (= C19), P29 (≠ D21), C52 (≠ H40), A142 (≠ G124), C225 (= C199), P226 (= P200), K227 (≠ D206)
- binding thiamine diphosphate: T50 (≠ E38), G51 (≠ P39), C52 (≠ H40), I74 (≠ H67), G109 (= G91), D110 (= D92), G111 (= G93), Y136 (≠ M118), N138 (= N120), S140 (≠ V122), Y141 (= Y123), A142 (≠ G124), N143 (≠ L125), T144 (= T126)
5exeC Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-tpp adduct (see paper)
22% identity, 93% coverage: 16:285/291 of query aligns to 24:298/314 of 5exeC
- active site: N143 (≠ L125)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-carboxy-4-methyl-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ E38), G51 (≠ P39), C52 (≠ H40), I74 (≠ H67), G109 (= G91), D110 (= D92), G111 (= G93), Y136 (≠ M118), N138 (= N120), S140 (≠ V122), Y141 (= Y123), A142 (≠ G124), N143 (≠ L125), T144 (= T126)
- binding magnesium ion: D110 (= D92), D130 (= D112), N138 (= N120), S140 (≠ V122), L211 (≠ I185), Q213 (≠ H187)
- binding iron/sulfur cluster: C24 (= C16), C27 (= C19), P29 (≠ D21), C52 (≠ H40), C225 (= C199), P226 (= P200), K227 (≠ D206)
5exdF Crystal structure of oxalate oxidoreductase from moorella thermoacetica bound with carboxy-di-oxido-methyl-tpp (coom-tpp) intermediate (see paper)
22% identity, 93% coverage: 16:285/291 of query aligns to 24:298/310 of 5exdF
- active site: N143 (≠ L125)
- binding magnesium ion: D110 (= D92), N138 (= N120), S140 (≠ V122)
- binding [2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-[1,1,2-tris(oxidanyl)-2-oxidanylidene-ethyl]-1,3-thiazol-3-ium-5-yl]ethoxy-oxidanyl-phosphoryl] hydrogen phosphate: T50 (≠ E38), G51 (≠ P39), C52 (≠ H40), I74 (≠ H67), D110 (= D92), G111 (= G93), Y136 (≠ M118), N138 (= N120), S140 (≠ V122), Y141 (= Y123), A142 (≠ G124), N143 (≠ L125), T144 (= T126)
- binding iron/sulfur cluster: C24 (= C16), C27 (= C19), P29 (≠ D21), C52 (≠ H40), A142 (≠ G124), C225 (= C199), K227 (≠ D206)
Query Sequence
>WP_012991706.1 NCBI__GCF_000025605.1:WP_012991706.1
MLKPADYRSDVEPTWCAGCGDFGVLAAITRAFAELGLEPHRVVSVSGIGCSSRLPLFLKN
YSIHTLHGRAIPVAIGVKLARPDLTVIVETGDGDLFSIGAGHNPHAARRNVDITVLCMDN
QVYGLTKNQVSPTSREWLYGSLTPYGSIDRPLNPIATMLAFGATFVAQTYAGNLQHMTYV
IKEAIKHRGFSFVNIISPCPTFNRVDTFQYYKGKVKDINQLGHDPSSYEAALRVAFHDLD
HYYDPEAPVPIGIFYRVQMETYEERMAKVKARYGGLEDLQEIIDACKPKRV
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory