SitesBLAST

Q89WV5 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) (see paper)
50% identity, 98% coverage: 11:630/630 of query aligns to 1:631/648 of Q89WV5

query
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Q89WV5

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G263 (= G266) mutation to I: Loss of activity.
G266 (= G269) mutation to I: Great decrease in activity.
K269 (= K272) mutation to G: Great decrease in activity.
E414 (= E417) mutation to Q: Great decrease in activity.

5jrhA Crystal structure of salmonella enterica acetyl-coa synthetase (acs) in complex with camp and coenzyme a (see paper)
50% identity, 98% coverage: 14:630/630 of query aligns to 2:627/640 of 5jrhA

query
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5jrhA

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active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N519), R522 (= R524), K605 (= K607)
binding (r,r)-2,3-butanediol: W93 (= W100), E140 (= E146), G169 (≠ L175), K266 (= K270), P267 (= P271)
binding adenosine-3',5'-cyclic-monophosphate: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D498), I508 (= I510), N517 (= N519), R522 (= R524)
binding coenzyme a: F159 (= F165), G160 (≠ A166), G161 (= G167), R187 (= R193), S519 (≠ A521), R580 (= R582), P585 (≠ A587)
binding magnesium ion: V533 (= V535), H535 (= H537), I538 (≠ V540)

Q8ZKF6 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
50% identity, 99% coverage: 9:630/630 of query aligns to 1:634/652 of Q8ZKF6

query
sites
Q8ZKF6

L

M

K

S

V

Q

Q

T

E

H

K

K

Y

H

Y

A

P

I

P

P

A

A

H

N

I

I

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A

E

D

R

R

A

C

W

L

V

I

-

N

-

P

K

E

D

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Y

Y

E

E

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T

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K

Y

Y

A

K

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Q

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I

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T

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F

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A

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A

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E

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E

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L

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D

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W

F

I

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T

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P

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Y

D

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K

V

V

L

K

D

N

W

T

Q

S

F

F

P

A

Y

P

A

G

-

N

-

V

-

S

-

I

Q

K

W

W

F

Y

V

E

G

D

A

G

K

T

T

L

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N

I

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A

Y

A

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N

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C

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L

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D

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-

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A

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L

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A

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A

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x
R

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A

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T

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L

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x
S

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G

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R

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G

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T

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A

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A

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A

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I

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Y

A

A

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Y

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V

I

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L

L

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N

K

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G

G

Y

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S

E

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E

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L

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Y

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A

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|
R

N

K

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E

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I

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G

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L

A

A

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P

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D

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E

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G

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|
K

I

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M

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L

L

K

R

A

K

Q

I

E

A

L

A

G

G

-

D

-

T

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N

V

L

G

G

D

D

I

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S

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L

L

E

A

D

D

R194 (≠ K196) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 3-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 2-fold reduction for CoA.
T311 (= T311) binding CoA
N335 (≠ D335) binding CoA
A357 (= A357) mutation to V: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 3-fold reduction for CoA.
D517 (= D515) mutation to G: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 10-fold reduction for CoA.; mutation to P: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold reduction in the affinity for ATP and 4.5-fold reduction for CoA.
S523 (≠ A521) binding CoA
G524 (= G522) mutation to L: No acetyl-coenzyme A synthetase activity.; mutation to S: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. Almost the same affinity as the wild-type for ATP, but 9-fold reduction in the affinity for CoA.
R526 (= R524) mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 4-fold reduction for CoA.
R584 (= R582) binding CoA; mutation to A: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 2-fold increase in the affinity for ATP and 7-fold reduction for CoA.; mutation to E: Results in a 2-fold reduction in the catalytic efficiency for both ATP and CoA. 3-fold increase in the affinity for ATP and 8-fold reduction for CoA.
K609 (= K607) modified: N6-acetyllysine; by Pat; mutation to A: No acetyl-coenzyme A synthetase activity.

2p20A Acetyl-coa synthetase, r584a mutation (see paper)
50% identity, 98% coverage: 14:630/630 of query aligns to 2:628/641 of 2p20A

query
sites
2p20A

K

K

Y

H

Y

A

P

I

P

P

A

A

H

N

I

I

V

A

E

D

R

R

A

C

W

L

V

I

-

N

-

P

K

E

D

Q

Y

Y

E

E

S

T

L

K

Y

Y

A

K

Q

Q

S

S

V

I

Q

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

K

E

V

Q

A

G

E

K

E

I

L

L

H

D

W

W

F

I

R

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

Q

S

F

F

P

A

Y

P

A

G

-

N

-

V

-

S

-

I

Q

K

W

W

F

Y

V

E

G

D

A

G

K

T

T

L

N

N

I

L

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A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

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-

L

L

Q

Q

G

E

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N

R

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T

A

A

Y

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G

E

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D

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K

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L

L

L

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V

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C

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F

A

A

N

N

G

T

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L

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L

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L

L

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K

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G

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A

A

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A

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M

L

L

A

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C

A

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G

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N

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L

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F

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I

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Y

T
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T

S

S

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G

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T

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G

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K

P

P

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K

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G

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L

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K

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D

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C

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G

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N

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E
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E

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G

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D

L

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I
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I

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R

A

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D

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L

L

N
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N

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R

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L

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G

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A

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A

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L

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L

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L

L

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P

K

K

T

T

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S

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G

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K

I

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M

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R

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L

L

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A

K

Q

I

E

A

L

A

G

G

L

D

P

T

V

S

G

N

D

D

I

T

S

S

T

T

L

L

E

A

D

D

active site: T260 (= T264), T412 (= T416), E413 (= E417), N517 (= N519), R522 (= R524), K605 (= K607)
binding adenosine-5'-monophosphate-propyl ester: G383 (= G387), E384 (= E388), P385 (= P389), T408 (= T412), W409 (= W413), W410 (= W414), Q411 (= Q415), T412 (= T416), D496 (= D498), I508 (= I510), R511 (= R513), R522 (= R524)

P9WQD1 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
50% identity, 99% coverage: 6:630/630 of query aligns to 3:640/651 of P9WQD1

query
sites
P9WQD1

E

E

V

S

L

T

L

P

K

E

V

V

Q

S

E

S

K

S

Y

Y

Y

P

P

P

A

A

H

H

I

F

V

A

E

E

R

H

A

A

W

N

V

A

K

R

D

-

Y

-

E

A

S

E

L

L

Y

Y

A

R

Q

E

S

A

V

E

Q

E

D

D

R

R

E

L

G

A

F

F

W

W

A

A

K

K

V

Q

A

A

E

N

E

R

L

L

H

S

W

W

F

T

R

T

K

P

W

F

D

T

K

E

V

V

L

L

D

D

W

W

Q

S

-

G

F

A

P

P

Y

F

A

A

Q

K

W

W

F

F

V

V

G

G

A

G

K

E

T

L

N

N

I

V

S

A

Y

Y

N

N

C

C

L

V

D

D

R

R

H

H

V

V

L

E

Q

A

G

G

R

H

R

G

N

D

K

R

V

V

A

A

Y

I

I

H

W

W

V

E

D

G

E

E

D

P

N

V

N

G

E

D

R

R

K

R

-

T

I

L

T

T

Y

Y

G

S

E

D

L

L

E

A

L

E

V

V

S

S

R

K

I

A

A

A

N

N

G

A

L

L

K

T

S

D

L

L

G

G

V

L

K

V

K

A

G

G

D

D

R

R

V

V

S

A

I

I

Y

Y

M

L

P

P

N

L

S

I

I

P

E

E

A

A

V

V

A

I

C

A

M

M

L

L

A

A

C

C

A

A

R

R

I

L

G

G

A

I

I

M

H

H

S

S

V

V

F

F

A

G

G

G

F

F

S

T

E

A

G

A

A

A

L

L

R

Q

L

A

R

R

I

I

E

V

D

D

A

A

K

Q

A

A

K

K

V

L

I

I

T

T

A

A

S

D

Y

G

T

Q

K

F

R

R

G

G

K

K

K

P

I

S

D

P

L

L

L

K

A

A

T

A

V

A

N

D

R

E

A

A

I

L

D

A

G

A

L

I

-

P

-

D

S

C

F

S

V

V

E

E

K

H

V

V

I

L

V

V

W

V

D

R

R

R

D

T

G

G

D

I

A

E

L

M

N

A

G

W

E

S

S

E

S

G

L

R

F

D

V

L

S

W

F

W

D

H

S

H

L

V

V

V

K

G

G

S

A

A

S

S

P

P

H

A

C

H

E

T

P

P

E

E

V

P

M

F

D

D

A

S

E

E

D

H

P

P

L

L

F

F

I

L

L

L

Y

Y

T

T

S

S

G

G

T

T

G

G

K

K

P

P

K

K

G

G

V

I

L

M

H

H

T

T

T

S

G

G

Y

Y

M

L

V

T

G

Q

T

C

Y

C

Y

Y

T

T

T

M

K

R

V

T

V

I

F

F

D

D

L

V

H

K

E

P

D

D

-

S

D

D

V

V

Y

F

W

W

C

C

T

T

A

A

D

D

I

I

G

G

W

W

I

V

T

T

G

G

H

H

S

T

Y

Y

I

G

V

V

Y

Y

G

G

P

P

L

L

A

C

N

N

G

G

A

V

T

T

S

E

I

V

I

L

V

Y

E

E

G

G

A

T

P

P

D

D

Y

T

P

P

D

D

P

R

G

H

R

R

W

H

W

F

S

Q

Y

I

V

I

E

E

K

K

Y

Y

R

G

V

V

N

T

I

I

F

Y

Y

Y

T

T

A

A

P

P

T

T

A

L

I

I

R

R

M

M

F

F

M

M

R

K

Y

W

G

G

E

R

Q

E

W

I

P

P

A

D

Q

S

Y

H

D

D

L

L

S

S

L

L

R

R

V

L

L

L

G

G

S

S

V

V

G

G

E

E

P

P

I

I

N

N

P

P

E

E

A

A

W

W

H

R

W

W

Y

Y

Y

R

K

D

H

V

I

I

G

G

R

G

E

G

R

R

C

T

V

P

I

L

V

V

D

D

T

T

W

W

Q

Q

T

T

E

E

T

T

G

G

A

S

H

A

M

M

I

I

T

S

T

P

V

L

P

P

S

G

Y

I

-

A

P

A

A

A

K

K

P

P

G

G

K

S

A

A

G

M

K

T

P

P

F

L

F

P

T

G

I

I

E

S

A

A

A

K

V

I

V

V

D

D

S

D

S

H

G

G

K

D

E

P

L

L

P

P

N

H

T

T

-

E

-

G

-

A

-

Q

-

H

-

V

V

T

G

G

N

Y

L

L

V

V

I

L

K

D

S

Q

P

P

W

W

P

P

S

S

M

M

L

L

R

R

T

G

C

I

W

W

G

G

E

D

P

P

E

A

R

R

Y

Y

-

W

E

H

K

S

Y

Y

W

W

N

S

T

K

I

F

P

S

-

D

-

K

G

G

Y

Y

L

F

T

A

G

G

D

D

L

G

A

A

S

R

Y

I

D

D

E

P

E

D

G

G

Y

A

I

I

M

W

I

V

L

L

G

G

R

R

A

I

D

D

V

V

L

M

N

N

V

V

A

S

G

G

H

H

R

R

I

I

G

S

T

T

M

A

E

E

V

V

E

E

S

S

A

A

L

L

V

V

D

A

H

H

P

S

A

G

V

V

A

A

E

E

A

A

V

V

I

V

G

G

K

V

P

T

H

D

E

E

I

T

K

T

G

T

E

Q

S

A

I

I

K

C

A

A

F

F

V

V

I

V

L

L

K

R

K

A

G

N

Y

Y

E

A

P

P

S

H

E

D

K

R

L

T

V

A

E

E

I

L

K

R

Q

T

H

E

V

V

R

A

N

R

V

V

L

I

G

S

A

P

I

I

A

A

V

R

P

P

D

R

E

D

I

V

E

H

F

V

V

V

E

P

K

E

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

V

L

L

L

K

R

A

D

Q

V

E

A

L

E

G

N

L

R

P

E

V

L

G

G

D

D

I

T

S

S

T

T

L

L

E

L

D

D

K617 (= K607) modified: N6-acetyllysine; mutation to R: Complete loss of acetyl-coenzyme A synthetase activity.

2p2fA Acetyl-coa synthetase, wild-type with acetate, amp, and coa bound (see paper)
50% identity, 98% coverage: 14:630/630 of query aligns to 1:624/637 of 2p2fA

query
sites
2p2fA

K

K

Y

H

Y

A

P

I

P

P

A

A

H

N

I

I

V

A

E

D

R

R

A

C

W

L

V

I

-

N

-

P

K

E

D

Q

Y

Y

E

E

S

T

L

K

Y

Y

A

K

Q

Q

S

S

V

I

Q

N

D

D

R

P

E

D

G

T

F

F

W

W

A

G

K

E

V

Q

A

G

E

K

E

I

L

L

H

D

W

W

F

I

R

T

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

Q

S

F

F

P

A

Y

P

A

G

-

N

-

V

-

S

-

I

Q

K

W

W

F

Y

V

E

G

D

A

G

K

T

T

L

N

N

I

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

V

-

L

L

Q

Q

G

E

R

N

R

G

N

D

K

R

V

T

A

A

Y

I

I

I

W

W

V

E

D

G

E

D

D

D

N

T

N

S

E

Q

R

S

K

K

-

H

I

I

T

S

Y

Y

G

R

E

E

L

L

L

H

E

R

L

D

V

V

S

C

R

R

I

F

A

A

N

N

G

T

L

L

K

L

S

D

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

S

A

I

I

Y

Y

M

M

P

P

N

M

S

V

I

P

E

E

A

A

V

A

A

V

C

A

M

M

L

L

A

A

C

C

A

A

R

R

I

I

G

G

A

A

I

V

H

H

S

S

V

V

V

I

F
|
F

A

G

G

G

F

F

S

S

E

P

G

E

A

A

L

V

R

A

L

G

R

R

I

I

E

I

D

D

A

S

K

S

A

S

K

R

V

L

V

V

I

I

T

T

A

A

S

D

Y

E

T

G

K

V

R
|
R

R

A

G

G

K

R

K

S

I

I

D

P

L

L

L

K

A

K

T

N

V

V

N

D

R

D

A

A

I

L

D

K

G

N

-

P

-

N

L

V

S

T

F

S

V

V

E

E

K

H

V

V

I

I

V

V

W

L

D

K

R

R

D

T

G

G

D

S

A

D

L

I

N

D

G

W

E

Q

S

E

S

G

L

R

F

D

V

L

S

W

F

W

D

R

S

D

L

L

V

I

K

E

G

K

A

A

S

S

P

P

H

E

C

H

E

Q

P

P

E

E

V

A

M

M

D

N

A

A

E

E

D

D

P

P

L

L

F

F

I

I

L

L

Y

Y

T
|
T

S

S

G

G

T

S

T

T

G

G

K

K

P

P

K

K

G

G

V

V

L

L

H

H

T

T

G

G

Y

Y

M

L

V

V

G

Y

T

A

Y

A

Y

T

T

T

T

F

K

K

V

Y

V

V

F

F

D

D

L

Y

H

H

E

P

D

G

D

D

V

I

Y

Y

W

W

C

C

T

T

A

A

D

D

I

V

G

G

W
|
W

I

V

T
|
T

G

G

H

H

S

S

Y

Y

I

L

V

L

Y

Y

G

G

P

P

L

L

A

A

N

C

G

G

A

A

T

T

S

T

I

L

I

M

V

F

E

E

G

G

A

V

P
|
P

D

N

Y

W

P

P

D

T

P

P

G

A

R

R

W

M

W

C

S

Q

Y

V

V

V

E

D

K

K

Y

H

R

Q

V

V

N

N

I

I

F

L

Y

Y

T

T

A
|
A

P

P

T

T

A
|
A

I

I

R

R

M

A

F

L

M

M

R

A

Y

E

G

G

E

D

Q

K

W

A

P

I

A

E

Q

G

Y

T

D

D

L

R

S

S

L

L

R

R

V

I

L

L

G

G

S

S

V

V

G
|
G

E
|
E

P
|
P

I

I

N

N

P

P

E

E

A

A

W

W

H

E

W

W

Y

Y

Y

W

K

K

H

K

I

I

G

G

R

K

E

E

R

K

C

C

V

P

I

V

V

V

D

D

T
|
T

W
|
W

Q
|
Q

T
|
T

E
|
E

T

T

G

G

A

G

H

F

M

M

I

I

T

T

T

P

V

L

P

P

-

G

S

A

Y

I

P

E

A

L

K

K

P

A

G

G

K

S

A

A

G

T

K

R

P

P

F

F

T

G

I

V

E

Q

A

P

A

A

V

L

V

V

D

D

S

N

S

E

G

G

K

H

E

P

L

Q

P

E

P

G

N

A

T

T

V

E

G

G

N

N

L

L

V

V

I

I

K

T

S

D

P

S

W

W

P

P

S

G

M

Q

L

A

R

R

T

T

C

L

W

F

G

G

E

D

P

H

E

E

R

R

Y

F

E

E

K

Q

-

T

Y

Y

W

F

N

S

T

T

I

F

P

K

G

N

Y

M

Y

Y

L

F

T

S

G

G

D
|
D

L

G

A

A

S

R

Y

R

D

D

E

E

E

D

G

G

Y

Y

I

Y

M

W

I
|
I

L

T

G

G

R
|
R

A

V

D

D

V

V

L

L

N
|
N

V

V

A
x
S

G

G

H

H

R
|
R

I

L

G

G

T

T

M

A

E

E

V

I

E

E

S

S

A

A

L

L

V

V

D

A

H

H

P

P

A

K

V

I

A

A

E

E

A

A

V

V

I

V

G

G

K

I

P

P

H

H

E

A

I

I

K

K

G

G

E

Q

S

A

I

I

K

Y

A

A

F

Y

V

V

I

T

L

L

K

N

K

H

G

G

Y

E

E

E

P

P

S

S

E

P

K

E

L

L

V

Y

E

A

E

E

I

V

K

R

Q

N

H

W

V

V

R
|
R

N

K

V

E

L

I

G

G

A
x
P

I

L

A

A

V

T

P

P

D

D

E

V

I

L

E

H

F

W

V

T

E

D

K

S

L

L

P

P

K

K

T

T

R

R

S

S

G

G

K
|
K

I

I

M

M

R

R

V

I

L

L

K

R

A

K

Q

-

E

-

L

-

G

-

L

I

P

A

V

A

G

G

D

D

I

T

-

S

S

S

T

T

L

L

E

A

D

D

active site: T259 (= T264), T411 (= T416), E412 (= E417), N516 (= N519), R521 (= R524), K604 (= K607)
binding adenosine monophosphate: G382 (= G387), E383 (= E388), P384 (= P389), T407 (= T412), W408 (= W413), W409 (= W414), Q410 (= Q415), T411 (= T416), D495 (= D498), I507 (= I510), R510 (= R513), N516 (= N519), R521 (= R524)
binding coenzyme a: F158 (= F165), R186 (= R193), W304 (= W309), T306 (= T311), P329 (= P334), A352 (= A357), A355 (= A360), S518 (≠ A521), R579 (= R582), P584 (≠ A587)

P27550 Acetyl-coenzyme A synthetase; AcCoA synthetase; Acs; Acetate--CoA ligase; Acyl-activating enzyme; EC 6.2.1.1 from Escherichia coli (strain K12) (see paper)
50% identity, 98% coverage: 14:630/630 of query aligns to 6:634/652 of P27550

query
sites
P27550

K

K

Y

H

Y

T

P

I

P

P

A

A

H

N

I

I

V

A

E

D

R

R

A

C

W

L

V

I

-

N

-

P

K

Q

D

Q

Y

Y

E

E

S

A

L

M

Y

Y

A

Q

Q

Q

S

S

V

I

Q

N

D

V

R

P

E

D

G

T

F

F

W

W

A

G

K

E

V

Q

A

G

E

K

E

I

L

L

H

D

W

W

F

I

R

K

K

P

W

Y

D

Q

K

K

V

V

L

K

D

N

W

T

Q

S

F

F

P

A

Y

P

A

G

-

N

-

V

-

S

-

I

Q

K

W

W

F

Y

V

E

G

D

A

G

K

T

T

L

N

N

I

L

S

A

Y

A

N

N

C

C

L

L

D

D

R

R

H

H

V

-

L

L

Q

Q

G

E

R

N

R

G

N

D

K

R

V

T

A

A

Y

I

I

I

W

W

V

E

D

G

E

D

D

D

N

A

N

S

E

Q

R

S

K

K

-

H

I

I

T

S

Y

Y

G

K

E

E

L

L

L

H

E

R

L

D

V

V

S

C

R

R

I

F

A

A

N

N

G

T

L

L

K

L

S

E

L

L

G

G

V

I

K

K

G

G

D

D

R

V

V

V

S

A

I

I

Y

Y

M

M

P

P

N

M

S

V

I

P

E

E

A

A

V

A

A

V