SitesBLAST
Comparing WP_012992045.1 NCBI__GCF_000025605.1:WP_012992045.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5tejB Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
41% identity, 100% coverage: 1:263/263 of query aligns to 1:266/269 of 5tejB
- active site: H155 (= H157), K159 (= K161)
- binding 2,5 Furan Dicarboxylic Acid: T100 (= T101), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ H35), F75 (= F75), T76 (≠ S76), S80 (≠ A81), G98 (= G99), T100 (= T101), P123 (= P124), N124 (= N125), Y125 (≠ M126), F239 (= F236)
5tejA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,5 furan dicarboxylic and nadh (see paper)
41% identity, 100% coverage: 1:263/263 of query aligns to 1:266/269 of 5tejA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ H35), F75 (= F75), T76 (≠ S76), S80 (≠ A81), G98 (= G99), T100 (= T101), P123 (= P124)
5temA Structure of 4-hydroxy-tetrahydrodipicolinate reductase from vibrio vulnificus with 2,6 pyridine dicarboxylic and nadh (see paper)
41% identity, 100% coverage: 1:263/263 of query aligns to 1:266/266 of 5temA
- active site: H155 (= H157), K159 (= K161)
- binding nicotinamide-adenine-dinucleotide: G8 (= G8), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), R35 (≠ H35), F75 (= F75), T76 (≠ S76), S80 (≠ A81), G98 (= G99), T100 (= T101), P123 (= P124), N124 (= N125), Y125 (≠ M126), F239 (= F236)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T101), P123 (= P124), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168)
4ywjA Crystal structure of 4-hydroxy-tetrahydrodipicolinate reductase (htpa reductase) from pseudomonas aeruginosa
41% identity, 100% coverage: 1:263/263 of query aligns to 1:267/268 of 4ywjA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G11 (= G11), R12 (= R12), M13 (= M13), D35 (≠ E34), R36 (≠ H35), F76 (= F75), T77 (≠ S76), V81 (≠ A81), G99 (= G99), T101 (= T101), A124 (≠ P124), N125 (= N125), F126 (≠ M126), R237 (= R233), F240 (= F236)
1drwA Escherichia coli dhpr/nhdh complex (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 7:269/272 of 1drwA
- active site: H158 (= H157), K162 (= K161)
- binding nicotinamide purin-6-ol-dinucleotide: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), E37 (= E34), R38 (≠ H35), F78 (= F75), T79 (≠ S76), R80 (≠ N78), G101 (= G99), T102 (= T100), T103 (= T101), A126 (≠ P124), N127 (= N125), F128 (≠ M126), F242 (= F236)
1dihA Three-dimensional structure of e. Coli dihydrodipicolinate reductase (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 7:269/272 of 1dihA
- active site: H158 (= H157), K162 (= K161)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), G14 (= G11), R15 (= R12), M16 (= M13), R38 (≠ H35), F78 (= F75), T79 (≠ S76), R80 (≠ N78), G83 (≠ A81), G101 (= G99), T103 (= T101), N127 (= N125), F128 (≠ M126), R239 (= R233), F242 (= F236)
P04036 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 99% coverage: 4:263/263 of query aligns to 8:270/273 of P04036
- G12 (= G8) binding NADP(+)
- GRM 15:17 (= GRM 11:13) binding NAD(+)
- RM 16:17 (= RM 12:13) binding NADP(+)
- E38 (= E34) binding NAD(+)
- R39 (≠ H35) binding NADP(+)
- T-R 80:81 (≠ SGN 76:78) binding NAD(+)
- GTT 102:104 (= GTT 99:101) binding NAD(+); binding NADP(+)
- AANF 126:129 (≠ SPNM 123:126) binding NAD(+)
- F129 (≠ M126) binding NADP(+)
- H159 (= H157) mutation H->A,Q: 135 to 200-fold reduction in catalytic activity.
- K163 (= K161) binding NAD(+); mutation K->A,C,Q: 625 to 830-fold reduction in catalytic activity.
- R240 (= R233) binding NADP(+)
- F243 (= F236) binding NAD(+)
1arzB Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 4:266/269 of 1arzB
- active site: H155 (= H157), K159 (= K161)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G8 (= G8), G10 (≠ L10), G11 (= G11), R12 (= R12), M13 (= M13), E34 (= E34), F75 (= F75), T76 (≠ S76), R77 (≠ N78), G80 (≠ A81), H84 (= H85), G98 (= G99), T100 (= T101), A123 (≠ P124), N124 (= N125), F125 (≠ M126), F239 (= F236)
- binding pyridine-2,6-dicarboxylic acid: T100 (= T101), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), F239 (= F236)
1drvA Escherichia coli dhpr/acnadh complex (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 5:267/270 of 1drvA
- active site: H156 (= H157), K160 (= K161)
- binding 3-acetylpyridine adenine dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), F76 (= F75), T77 (≠ S76), R78 (≠ N78), G81 (≠ A81), G99 (= G99), A124 (≠ P124), F126 (≠ M126), R237 (= R233)
1druA Escherichia coli dhpr/nadh complex (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 5:267/270 of 1druA
- active site: H156 (= H157), K160 (= K161)
- binding nicotinamide-adenine-dinucleotide: G9 (= G8), G12 (= G11), R13 (= R12), M14 (= M13), E35 (= E34), R36 (≠ H35), F76 (= F75), T77 (≠ S76), R78 (≠ N78), G81 (≠ A81), G99 (= G99), T100 (= T100), T101 (= T101), A124 (≠ P124), N125 (= N125), F126 (≠ M126), F240 (= F236)
1arzA Escherichia coli dihydrodipicolinate reductase in complex with nadh and 2,6 pyridine dicarboxylate (see paper)
40% identity, 99% coverage: 4:263/263 of query aligns to 5:267/270 of 1arzA
3ijpA Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
38% identity, 99% coverage: 4:263/263 of query aligns to 4:266/266 of 3ijpA
- active site: H155 (= H157), K159 (= K161)
- binding sodium ion: I21 (≠ S21), Q22 (≠ L22), R24 (≠ Y24), V27 (= V27)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), N10 (≠ L10), G11 (= G11), R12 (= R12), M13 (= M13), R35 (vs. gap), F75 (= F75), S76 (= S76), Q77 (≠ N78), A80 (= A81), G98 (= G99), T100 (= T101), G123 (≠ P124), N124 (= N125), M125 (= M126), F239 (= F236)
3ijpB Crystal structure of dihydrodipicolinate reductase from bartonella henselae at 2.0a resolution (see paper)
38% identity, 99% coverage: 4:263/263 of query aligns to 4:266/267 of 3ijpB
Q9X1K8 4-hydroxy-tetrahydrodipicolinate reductase; HTPA reductase; EC 1.17.1.8 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
42% identity, 73% coverage: 70:261/263 of query aligns to 43:212/216 of Q9X1K8
Sites not aligning to the query:
1vm6B Crystal structure of dihydrodipicolinate reductase (tm1520) from thermotoga maritima at 2.27 a resolution
42% identity, 73% coverage: 71:261/263 of query aligns to 49:217/218 of 1vm6B
- active site: H132 (= H157), K136 (= K161)
- binding nicotinamide-adenine-dinucleotide: F53 (= F75), S54 (= S76), S55 (≠ N78), E57 (= E80), A58 (= A81), G76 (= G99), T78 (= T101), Y101 (≠ P124), N102 (= N125), F103 (≠ M126), F192 (= F236)
Sites not aligning to the query:
5wolA Crystal structure of dihydrodipicolinate reductase dapb from coxiella burnetii
29% identity, 93% coverage: 3:247/263 of query aligns to 3:216/230 of 5wolA
- active site: H133 (= H157), K137 (= K161)
- binding pyridine-2-carboxylic acid: P104 (= P124), T144 (= T168), K147 (≠ R171)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: N7 (≠ C7), G11 (= G11), K12 (≠ R12), M13 (= M13), G34 (≠ E34), R35 (≠ H35), F54 (= F75), T55 (≠ S76), T56 (≠ N78), S59 (≠ A81), G77 (= G99), T78 (= T100), T79 (= T101), P104 (= P124), N105 (= N125), F106 (≠ M126)
5z2fA NADPH/pda bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
26% identity, 99% coverage: 1:261/263 of query aligns to 2:265/265 of 5z2fA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R11 (≠ L10), G12 (= G11), K13 (≠ R12), M14 (= M13), D35 (≠ E34), H36 (= H35), K37 (≠ P36), L76 (≠ F75), T77 (≠ S76), G99 (= G99), T100 (= T100), T101 (= T101), P126 (= P124), N127 (= N125), F128 (≠ M126)
- binding pyridine-2,6-dicarboxylic acid: P126 (= P124), H155 (= H157), H156 (= H158), K159 (= K161), S164 (= S166), G165 (= G167), T166 (= T168), A215 (≠ G211)
5z2eA Dipicolinate bound dihydrodipicolinate reductase from paenisporosarcina sp. Tg-14 (see paper)
26% identity, 99% coverage: 1:261/263 of query aligns to 2:265/265 of 5z2eA
5eesA Crystal structure of dapb in complex with NADP+ from corynebacterium glutamicum (see paper)
30% identity, 99% coverage: 3:263/263 of query aligns to 3:247/247 of 5eesA
- active site: H133 (= H157), K137 (= K161)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G11 (= G11), R12 (= R12), V13 (≠ M13), G34 (≠ E34), V35 (≠ H35), F53 (= F75), T54 (≠ S76), G76 (= G99), T78 (= T101), P104 (= P124), N105 (= N125), F106 (≠ M126), F220 (= F236)
- binding sulfate ion: H134 (= H158), K137 (= K161), K137 (= K161), G143 (= G167), T144 (= T168)
5eerA Crystal structure of dapb from corynebacterium glutamicum (see paper)
30% identity, 99% coverage: 3:263/263 of query aligns to 3:247/247 of 5eerA
Query Sequence
>WP_012992045.1 NCBI__GCF_000025605.1:WP_012992045.1
MTEVVLCGALGRMGKAILRLSLEYQDVRVVAGVEHPDCLSSTDLGQASGIREFTGVPLTS
RLEEVIGLGQVVLDFSGNPEAAVGHARLAAAYGKGVVIGTTGLERSHLEDLRELSKDIPV
LVSPNMSLGVNLLIKLVELATKVLKEKGFDVEILEIHHRYKKDAPSGTALRLAEVIRNEL
GDVKLVSGREGIAPRGEEVGVMALRGGDVVGEHTVFFIGNGERLELIHRATSRDIFARGA
IEACRWIASQKPGFYSMMEVLGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory