SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012992228.1 NCBI__GCF_000025605.1:WP_012992228.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P14218 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex; EC 1.8.1.4 from Pseudomonas fluorescens (see 2 papers)
34% identity, 98% coverage: 2:453/463 of query aligns to 4:466/478 of P14218

query
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P14218

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34:49 (vs. 32:42, 31% identical) binding FAD
C49 (= C42) modified: Disulfide link with 54, Redox-active
C54 (= C47) modified: Disulfide link with 49, Redox-active
K58 (= K51) binding FAD
G122 (= G114) binding FAD
D319 (= D305) binding FAD
A327 (= A313) binding FAD

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5u8uD Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa (see paper)
34% identity, 98% coverage: 2:453/463 of query aligns to 6:468/477 of 5u8uD

query
sites
5u8uD

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active site: P16 (≠ S12), L47 (≠ V38), C51 (= C42), C56 (= C47), S59 (= S50), G85 (= G75), V86 (≠ F76), V193 (≠ A180), E197 (= E184), S333 (≠ M317), F451 (≠ Y436), H453 (= H438), E458 (= E443)
binding flavin-adenine dinucleotide: I12 (≠ V8), G15 (= G11), P16 (≠ S12), G17 (= G13), E36 (= E32), K37 (vs. gap), G49 (= G40), T50 (≠ N41), C51 (= C42), G55 (= G46), C56 (= C47), K60 (= K51), H123 (≠ K113), G124 (= G114), A152 (= A139), S153 (≠ T140), G154 (= G141), I194 (≠ V181), R281 (= R267), G320 (= G304), D321 (= D305), M327 (= M311), L328 (= L312), A329 (= A313), H330 (= H314), H453 (= H438), P454 (= P439)

Sites not aligning to the query:

active site: 476, 477

5u8vA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to NAD+ (see paper)
34% identity, 98% coverage: 2:453/463 of query aligns to 2:464/472 of 5u8vA

query
sites
5u8vA

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V

A

A

V
|
V

G
|
G

R
|
R

K

R

P

P

N

V

T

T

E

T

G

D

F

L

-

L

-

A

K

A

E

D

T

S

G

G

I

V

E

T

M

L

D

D

E

E

R

R

G

G

F

F

V

I

V

Y

V

V

D

D

K

D

H

H

C

C

R

K

T

T

N

S

L

V

P

P

S

G

V

V

Y

F

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

R

P

G

L

A

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M
x
S

Y

E

E

E

G

G

K

-

V

V

A

M

V

V

S

A

H

E

M

R

L

I

G

A

D

G

E

H

G

K

W

A

E

Q

K

M

N

N

D

Y

T

D

L

L

M

I

P

P

K

S

I

V

I

I

Y

Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

D

Q

E

T

A

L

E

K

D

A

E

E

G

G

Y

V

H

E

V

V

R

N

V

V

G

G

V

T

V

F

S

P

F

F

V

A

T

A

N

S

P

G

K

R

A

A

M

M

D

A

G

N

E

D

N

T

E

T

G

G

F

L

V

V

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

V

L

L

G

G

C

V

H

H

I

V

L

I

G

G

P

P

N

S

A

A

G

A

E

E

L

L

L

V

H

Q

Q

Q

V

G

I

A

H

I

A

G

M

M

K

E

A

F

G

G

L

T

G

S

A

A

D

E

F

D

M

L

S

G

R

M

S

M

V

V

Y
x
F

S

S

H
|
H

P
|
P

S

T

L

L

S

S

E
|
E

A

A

I

L

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

H

N

active site: P12 (≠ S12), L43 (≠ V38), C47 (= C42), C52 (= C47), S55 (= S50), G81 (= G75), V82 (≠ F76), V189 (≠ A180), E193 (= E184), S329 (≠ M317), F447 (≠ Y436), H449 (= H438), E454 (= E443)
binding flavin-adenine dinucleotide: I8 (≠ V8), G11 (= G11), P12 (≠ S12), G13 (= G13), E32 (= E32), G45 (= G40), T46 (≠ N41), C47 (= C42), G51 (= G46), C52 (= C47), K56 (= K51), H119 (≠ K113), G120 (= G114), A148 (= A139), S149 (≠ T140), G150 (= G141), S169 (≠ T160), I190 (≠ V181), R277 (= R267), G316 (= G304), D317 (= D305), M323 (= M311), L324 (= L312), A325 (= A313), H326 (= H314), H449 (= H438), P450 (= P439)
binding nicotinamide-adenine-dinucleotide: I185 (≠ V176), G186 (= G177), G188 (= G179), V189 (≠ A180), I190 (≠ V181), L208 (≠ T199), E209 (= E200), A210 (≠ L201), V243 (= V236), V275 (= V265), G276 (= G266)

Sites not aligning to the query:

active site: 472

5u8wA Dihydrolipoamide dehydrogenase (lpdg) from pseudomonas aeruginosa bound to nadh (see paper)
34% identity, 98% coverage: 2:453/463 of query aligns to 3:465/473 of 5u8wA

query
sites
5u8wA

E

K

F

F

D

D

L

V

V

V

I

V

V
x
I

G

G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

L

K

K

V

T

A

A

F

C

V

I

E
|
E

-
x
K

-

Y

L

I

S

G

P

K

E

E

-

G

-

K

-

V

T

A

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

D

G

S

A

S

Y

Y

M

K

I

Y

-

H

E

E

R

A

F

K

S

E

H

A

M

F

S

K

R

V

Y

H

G

G

I

I

L

E

P

A

K

K

G
|
G

F
x
V

H

T

L

I

S

D

M

V

P

P

H

A

L

M

K

V

E

A

G

R

R

K

D

A

N

N

V

I

V

V

R

K

T

N

I

L

R

T

E

G

N

G

F

I

K

A

K

T

F

L

A

F

Q

K

H

A

L

N

G

G

I

V

P

T

I

S

F

F

Y

E

G

G

K
x
H

G
|
G

F

K

L

L

K

L

D

A

P

N

N

K

T

Q

V

V

V

E

V

V

E

T

G

G

A

L

D

D

-

G

-

K

-

T

I

Q

T

V

L

L

R

E

S

A

R

E

F

N

V

V

L

I

A
|
A

T
x
S

G
|
G

S

S

S

R

T

P

V

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S

E

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I

G

P

M

A

V

P

A

L

D

T

G

D

R

D

Y

I

I

I

H

V

D

D

T
x
S

D

T

T

G

I

A

W

L

N

E

L

F

E

Q

E

A

F

V

P

P

K

K

R

K

V

L

V

G

I

V

V
x
I

G

G

G

A

G
|
G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

A

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

V

T

L

E
|
E

L
x
A

K
x
L

D

D

R

K

L

F

L

L

P

P

M

A

A

A

G

-

I

-

P

D

E

E

E

Q

S

I

S

A

R

K

F

E

L

A

A

L

R

K

K

V

L

L

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T

K

K

L

Q

G

G

I

L

D

N

I

I

K

R

L

L

K

G

T

A

T

R

V

V

N

T

H

A

Y

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E

E

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T

K

E

K

G

Q

L

V

K

T

V

V

S

T

F

F

T

T

D

D

G

A

T

N

-

G

-

E

-

Q

Q

K

L

E

T

T

T

F

D

D

I

K

I

L

L

I

L

V

A
|
A

V
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V

G
|
G

R
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R

K

R

P

P

N

V

T

T

E

T

G

D

F

L

-

L

-

A

K

A

E

D

T

S

G

G

I

V

E

T

M

L

D

D

E

E

R

R

G

G

F

F

V

I

V

Y

V

V

D

D

K

D

H

H

C

C

R

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T

T

N

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L

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P

P

S

G

V

V

Y

F

A

A

C

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D
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D

I

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V

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R

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G

L

A

M
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M

L
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L

A
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A

H
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H

K

K

A

A

M
x
S

Y

E

E

E

G

G

K

-

V

V

A

M

V

V

S

A

H

E

M

R

L

I

G

A

D

G

E

H

G

K

W

A

E

Q

K

M

N

N

D

Y

T

D

L

L

M

I

P

P

K

S

I
x
V

I

I

Y
|
Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

D

Q

E

T

A

L

E

K

D

A

E

E

G

G

Y

V

H

E

V

V

R

N

V

V

G

G

V

T

V

F

S

P

F

F

V

A

T

A

N

S

P

G

K

R

A

A

M

M

D

A

G

N

E

D

N

T

E

T

G

G

F

L

V

V

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

V

L

L

G

G

C

V

H

H

I

V

L

I

G

G

P

P

N

S

A

A

G

A

E

E

L

L

L

V

H

Q

Q

Q

V

G

I

A

H

I

A

G

M

M

K

E

A

F

G

G

L

T

G

S

A

A

D

E

F

D

M

L

S

G

R

M

S

M

V

V

Y
x
F

S

S

H
|
H

P
|
P

S

T

L

L

S

S

E
|
E

A

A

I

L

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

H

N

active site: P13 (≠ S12), L44 (≠ V38), C48 (= C42), C53 (= C47), S56 (= S50), G82 (= G75), V83 (≠ F76), V190 (≠ A180), E194 (= E184), S330 (≠ M317), F448 (≠ Y436), H450 (= H438), E455 (= E443)
binding flavin-adenine dinucleotide: I9 (≠ V8), G12 (= G11), P13 (≠ S12), G14 (= G13), E33 (= E32), K34 (vs. gap), G46 (= G40), T47 (≠ N41), C48 (= C42), G52 (= G46), C53 (= C47), K57 (= K51), H120 (≠ K113), G121 (= G114), A149 (= A139), S150 (≠ T140), G151 (= G141), S170 (≠ T160), G317 (= G304), D318 (= D305), M324 (= M311), L325 (= L312), A326 (= A313), H327 (= H314), Y357 (= Y345), H450 (= H438), P451 (= P439)
binding 1,4-dihydronicotinamide adenine dinucleotide: I186 (≠ V176), G189 (= G179), V190 (≠ A180), I191 (≠ V181), E194 (= E184), E210 (= E200), A211 (≠ L201), L212 (≠ K202), A275 (= A264), V276 (= V265), G277 (= G266), R278 (= R267), M324 (= M311), L325 (= L312), V355 (≠ I343), Y357 (= Y345)

Sites not aligning to the query:

active site: 473

3urhB Crystal structure of a dihydrolipoamide dehydrogenase from sinorhizobium meliloti 1021
35% identity, 98% coverage: 3:457/463 of query aligns to 1:464/465 of 3urhB

query
sites
3urhB

F

Y

D

D

L

L

V

I

I

V

V
x
I

G
|
G

A

S

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

C

G

A

L

I

Y

K

A

A

H

A

R

Q

R

L

G

G

M

M

K

K

V

V

A

A

F

V

V

V

E
|
E

L

-

S
x
K

P

R

E

S

T

T

V
x
Y

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

H

G

A

A

S

Y

E

M

M

I

F

E

-

R

-

F

-

S

-

H

H

M

Q

S

A

R

Q

Y

H

G

G

I

L

L

E

P

A

K

L

G

G

F

V

H

E

L

V

S

A

M

N

P

P

H

K

L

L

K

N

-

L

-

Q

-

K

-

M

E

A

G

H

R

K

D

D

N

A

V

T

V

V

R

K

T

S

I

N

R

V

E

D

N

G

F

V

K

S

K

F

F

L

A

F

Q

K

H

K

L

N

G

K

I

I

P

D

I

G

F

F

Y

Q

G

G

K
x
T

G
|
G

F

K

L

V

K

L

D

G

P

Q

N

G

T

K

V

V

V

S

V

V

-

T

-

N

-

E

E

K

G

G

A

E

D

E

I

Q

T

V

L

L

R

E

S

A

R

K

F

N

V

V

L

V

L

I

A
|
A

T
|
T

G
|
G

S

S

S

D

T

V

V

A

S

G

V

I

G

P

G

G

M

V

-

E

V

V

A

A

-

F

D

D

G

E

R

K

Y

T

I

I

H

V

D

S

T

S

D

T

T

G

I

A

W

L

N

A

L

L

E

E

E

K

F

V

P

P

K

A

R

S

V

M

V

I

I

V

V

V

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

A

M

R

Y

L

G

G

A

A

D

K

V

V

T

T

L

V

T

V

E

E

L

F

K

L

D

D

R

T

L

I

L

L

P

-

M

-

A

G

G

G

I

M

P

D

E

G

E

E

S

V

S

A

R

K

F

Q

L

L

A

Q

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R

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L

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T

K

K

L

Q

G

G

I

I

D

D

I

F

K

K

L

L

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A

T

K

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V

N

T

H

G

Y

A

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D

G

G

L

A

K

K

V

V

S

T

F

F

T

E

-

P

-

V

-

K

-

G

D

E

G

A

T

T

Q

T

L

L

T

D

T

A

D

E

I

V

L

L

L

I

A

A

V

T

G

G

R
|
R

K

K

P

P

N

S

T

T

E

D

G

G

F

L

-

G

-

L

K

A

E

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G

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V

M

L

D

D

E

S

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R

G

G

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V

V

E

V

I

D

D

K

R

H

H

C

F

R

Q

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T

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A

S

G

V

V

Y

Y

A

A

C

I

G
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G

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D

I

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R

P

G

L

P

M
|
M

L
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L

A
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H
|
H

K

K

A

A

M

E

Y

D

E

E

G

G

K

-

V

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A

A

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S

A

H

E

M

I

L

I

G

A

D

G

E

Q

G

A

W

G

E

H

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V

N

N

D

Y

T

D

L

V

M

I

P

P

K

G

I

V

Y

Y

S

T

A

Q

Y

P

E

E

V

V

A

A

S

S

V

V

G

G

L

K

T

T

E

E

D

E

E

E

A

L

E

K

D

A

E

A

G

G

Y

V

H

A

V

Y

R

K

V

I

G

G

V

K

V

F

S

P

F

F

V

T

T

A

N

N

P

G

K

R

A

A

M

R

D

A

D

M

G

L

E

Q

N

T

E

D

G

G

F

F

V

V

R

K

V

I

V

L

V

A

E

D

E

K

R

E

T

T

K

D

R

R

I

V

L

L

G

G

C

G

H

H

I

I

L

I

G

G

P

F

N

G

A

A

G

G

E

E

L

M

L

I

H

H

Q

E

V

I

I

A

H

V

A

L

M

M

K

E

A

F

G

G

L

G

G

S

A

S

D

E

F

D

M

L

S

G

R

R

S

T

V

C

Y
x
H

S

A

H
|
H

P

P

S

T

L

M

S

S

E
|
E

A

A

I

V

G

K

Q

E

A

A

S

A

M

L

E

S

V

T

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F

F

F

G

K

P

P

I

I

active site: Y35 (≠ V38), C39 (= C42), C44 (= C47), S47 (= S50), V183 (≠ A180), E187 (= E184), H443 (≠ Y436), H445 (= H438), E450 (= E443)
binding flavin-adenine dinucleotide: I6 (≠ V8), G7 (= G9), G9 (= G11), P10 (≠ S12), G11 (= G13), E30 (= E32), K31 (≠ S34), G37 (= G40), T38 (≠ N41), C39 (= C42), G43 (= G46), C44 (= C47), K48 (= K51), T111 (≠ K113), G112 (= G114), A140 (= A139), T141 (= T140), G142 (= G141), I184 (≠ V181), R273 (= R267), G312 (= G304), D313 (= D305), M319 (= M311), L320 (= L312), A321 (= A313), H322 (= H314)

3ladA Refined crystal structure of lipoamide dehydrogenase from azotobacter vinelandii at 2.2 angstroms resolution. A comparison with the structure of glutathione reductase (see paper)
33% identity, 97% coverage: 2:452/463 of query aligns to 3:464/472 of 3ladA

query
sites
3ladA

E

K

F

F

D

D

L

V

V

I

I

V

V
x
I

G
|
G

A

A

G
|
G

S
x
P

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

K

A

S

H

A

R

Q

R

L

G

G

M

L

K

K

V

T

A

A

F

L

V

I

E
|
E

L
x
K

-

Y

-

K

-

G

-

K

-

E

S

G

P

K

E

T

T

A

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K

K

Y

A

M

L

R

L

Y

D

G

S

A

S

Y

Y

M

K

I

F

-

H

E

E

R

A

F

H

S

E

H

S

M

F

S

K

R

L

Y

H

G

G

I

I

L

S

P

T

K

G

G

E

F

V

H

A

L

I

S

D

M

V

P

P

H

T

L

M

K

I

E

A

G

R

R

K

D

D

N

Q

V

I

V

V

R

R

T

N

I

L

R

T

E

G

N

G

F

V

K

A

K

S

F

L

A

I

Q

K

H

A

L

N

G

G

I

V

P

T

I

L

F

F

Y

E

G

G

K
x
H

G
|
G

F

K

L

L

-

L

-

A

-

G

K

K

D

K

P

V

N

E

T

V

V

T

V

A

E

D

G

G

A

S

D

S

I

Q

T

V

L

L

R

D

S

T

R

E

F

N

V

V

L

I

L

L

A
|
A

T
x
S

G
|
G

S

S

S

K

T

P

V

V

S

E

V

I

G

P

M

A

V

P

A

V

D

D

G

Q

R

D

Y

V

I

I

H

V

D

D

T

S

D

T

T

G

I

A

W

L

N

D

L

F

E

Q

E

N

F

V

P

P

K

G

R

K

V

L

V

G

I

V

V

I

G

G

G

A

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

A

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

V

T

L

E

E

L

A

K

M

D

D

R

K

L

F

L

L

P

P

M

-

A

-

G

A

I

V

P

D

E

E

E

Q

S

V

S

A

R

K

F

E

L

A

A

Q

R

K

K

I

L

L

K

T

K

K

L

Q

G

G

I

L

D

K

I

I

K

L

L

L

K

G

T

A

T

R

V

V

N

T

H

G

Y

T

E

E

K

V

T

K

E

N

E

K

G

Q

L

V

K

T

V

V

S

K

F

F

T

V

D

D

G

A

-

E

-

G

-

E

T

K

Q

S

L

Q

T

A

T

F

D

D

I

K

I

L

L

I

L

V

A

A

V

V

G

G

R
|
R

K

R

P

P

N

V

T

T

E

T

G

D

F

L

-

L

-

A

K

A

E

D

T

S

G

G

I

V

E

T

M

L

D

D

E

E

R

R

G

G

F

F

V

I

V

Y

V

V

D

D

K

D

H

Y

C

C

R

A

T

T

N

S

L

V

P

P

S

G

V

V

Y

Y

A

A

C

I

G

G

D
|
D

I

V

T

V

S

R

P

G

L

A

M

M

L
|
L

A
|
A

H

H

K

K

A

A

M

S

Y

E

E

E

G

G

K

V

V

V

A

V

V

A

S

E

H

R

M

I

L

A

G

G

D

H

E

K

G

A

W

-

E

Q

K

M

N

N

D

Y

T

D

L

L

M

I

P

P

K

A

I

V

I

I

Y

Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

G

V

V

G

G

L

K

T

T

E

E

D

Q

E

A

A

L

E

K

D

A

E

E

G

G

Y

V

H

A

V

I

R

N

V

V

G

G

V

V

V

F

S

P

F

F

V

A

T

A

N

S

P

G

K

R

A

A

M

M

D

A

G

N

E

D

N

T

E

A

G

G

F

F

V

V

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

V

L

L

G

G

C

V

H

H

I

V

L

I

G

G

P

P

N

S

A

A

G

A

E

E

L

L

L

V

H

Q

Q

Q

V

G

I

A

H

I

A

A

M

M

K

E

A

F

G

G

L

T

G

S

A

A

D

E

F

D

M

L

S

G

R

M

S

M

V

V

Y
x
F

S

A

H
|
H

P

P

S

A

L

L

S

S

E
|
E

A

A

I

L

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

active site: L44 (≠ V38), C48 (= C42), C53 (= C47), S56 (= S50), V190 (≠ A180), E194 (= E184), F448 (≠ Y436), H450 (= H438), E455 (= E443)
binding flavin-adenine dinucleotide: I9 (≠ V8), G10 (= G9), G12 (= G11), P13 (≠ S12), E33 (= E32), K34 (≠ L33), G46 (= G40), T47 (≠ N41), C48 (= C42), G52 (= G46), C53 (= C47), H120 (≠ K113), G121 (= G114), A149 (= A139), S150 (≠ T140), G151 (= G141), I191 (≠ V181), R278 (= R267), D318 (= D305), L325 (= L312), A326 (= A313)

P18925 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Azotobacter vinelandii (see 2 papers)
33% identity, 97% coverage: 2:452/463 of query aligns to 4:465/477 of P18925

query
sites
P18925

E

K

F

F

D

D

L

V

V

I

I

V

V

I

G

G

A

A

G

G

S

P

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

K

A

S

H

A

R

Q

R

L

G

G

M

L

K

K

V

T

A

A

F

L

V

I

E
|
E

L
x
K

-
x
Y

-
x
K

-
x
G

-
x
K

-
x
E

S
x
G

P
x
K

E
x
T

T
x
A

V
x
L

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G

G

C
|
C

I

I

P

P

S

S

K
|
K

Y

A

M

L

R

L

Y

D

G

S

A

S

Y

Y

M

K

I

F

-

H

E

E

R

A

F

H

S

E

H

S

M

F

S

K

R

L

Y

H

G

G

I

I

L

S

P

T

K

G

G

E

F

V

H

A

L

I

S

D

M

V

P

P

H

T

L

M

K

I

E

A

G

R

R

K

D

D

N

Q

V

I

V

V

R

R

T

N

I

L

R

T

E

G

N

G

F

V

K

A

K

S

F

L

A

I

Q

K

H

A

L

N

G

G

I

V

P

T

I

L

F

F

Y

E

G

G

K

H

G

G

F

K

L

L

-

L

-

A

-

G

K

K

D

K

P

V

N

E

T

V

V

T

V

A

E

D

G

G

A

S

D

S

I

Q

T

V

L

L

R

D

S

T

R

E

F

N

V

V

L

I

L

L

A

A

T

S

G

G

S

S

S

K

T

P

V

V

S

E

V

I

G

P

M

A

V

P

A

V

D

D

G

Q

R

D

Y

V

I

I

H

V

D

D

T

S

D

T

T

G

I

A

W

L

N

D

L

F

E

Q

E

N

F

V

P

P

K

G

R

K

V

L

V

G

I

V

V

I

G

G

G

A

G

G

A

V

V

I

G

G

V

L

E

E

F

L

A

G

Y

S

I

V

F

W

R

A

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

V

T

L

E

E

L

A

K

M

D

D

R

K

L

F

L

L

P

P

M

-

A

-

G

A

I

V

P

D

E

E

E

Q

S

V

S

A

R

K

F

E

L

A

A

Q

R

K

K

I

L

L

K

T

K

K

L

Q

G

G

I

L

D

K

I

I

K

L

L

L

K

G

T

A

T

R

V

V

N

T

H

G

Y

T

E

E

K

V

T

K

E

N

E

K

G

Q

L

V

K

T

V

V

S

K

F

F

T

V

D

D

G

A

-

E

-

G

-

E

T

K

Q

S

L

Q

T

A

T

F

D

D

I

K

I

L

L

I

L

V

A

A

V

V

G

G

R

R

K

R

P

P

N

V

T

T

E

T

G

D

F

L

-

L

-

A

K

A

E

D

T

S

G

G

I

V

E

T

M

L

D

D

E

E

R

R

G

G

F

F

V

I

V

Y

V

V

D

D

K

D

H

Y

C

C

R

A

T

T

N

S

L

V

P

P

S

G

V

V

Y

Y

A

A

C

I

G

G

D
|
D

I

V

T

V

S

R

P

G

L

A

M

M

L

L

A
|
A

H

H

K

K

A

A

M

S

Y

E

E

E

G

G

K

V

V

V

A

V

V

A

S

E

H

R

M

I

L

A

G

G

D

H

E

K

G

A

W

-

E

Q

K

M

N

N

D

Y

T

D

L

L

M

I

P

P

K

A

I

V

I

I

Y

Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

G

V

V

G

G

L

K

T

T

E

E

D

Q

E

A

A

L

E

K

D

A

E

E

G

G

Y

V

H

A

V

I

R

N

V

V

G

G

V

V

V

F

S

P

F

F

V

A

T

A

N

S

P

G

K

R

A

A

M

M

D

A

G

N

E

D

N

T

E

A

G

G

F

F

V

V

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

V

L

L

G

G

C

V

H

H

I

V

L

I

G

G

P

P

N

S

A

A

G

A

E

E

L

L

L

V

H

Q

Q

Q

V

G

I

A

H

I

A

A

M

M

K

E

A

F

G

G

L

T

G

S

A

A

D

E

F

D

M

L

S

G

R

M

S

M

V

V

Y

F

S

A

H

H

P

P

S

A

L

L

S

S

E

E

A

A

I

L

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

34:49 (vs. 32:42, 25% identical) binding FAD
C49 (= C42) modified: Disulfide link with 54, Redox-active
C54 (= C47) modified: Disulfide link with 49, Redox-active
K58 (= K51) binding FAD
D319 (= D305) binding FAD
A327 (= A313) binding FAD

6bz0A 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from acinetobacter baumannii in complex with fad.
34% identity, 97% coverage: 2:452/463 of query aligns to 1:461/469 of 6bz0A

query
sites
6bz0A

E

Q

F

F

D

D

L

L

V

V

I

V

V
x
I

G

G

A

G

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

E

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

F

K

K

V

V

A

A

F

C

V

I

E
|
E

L
x
K

-
x
R

-

I

-

H

-

N

S

G

P

K

E

P

T

S

V

L

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

D

G

S

A

S

Y

H

M

R

I

Y

E

E

-

D

R

T

F

V

S

H

H

H

M

L

S

A

R

D

Y

H

G

G

I

I

L

T

P

T

K

G

G

E

F

V

H

N

L

F

S

D

M

L

P

A

H

K

L

L

K

L

E

A

G

R

R

K

D

D

N

K

V

I

V

V

R

D

T

Q

I

L

R

T

E

G

N

G

F

I

K

D

K

Q

F

L

A

L

Q

K

H

G

L

N

G

G

I

I

P

E

I

W

F

L

Y

K

G

G

K
x
T

G
|
G

F

K

L

L

-

L

-

A

-

G

K

K

D

K

P

V

N

E

T

F

V

V

V

P

V

H

E

E

G

G

A

E

D

T

I

Q

T

I

L

L

R

E

S

P

R

K

F

Y

V

V

L

I

L

L

A

A

T
x
S

G
|
G

S

S

S

V

T

P

V

V

S

N

V

I

G

P

G

V

M

A

V

P

A

V

D

D

G

Q

R

D

Y

I

I

I

H

V

D

D

T
x
S

D

T

T

G

I

A

W

L

N

N

L

F

E

P

E

E

F

V

P

P

K

K

R

R

V

L

V

G

I

V

V

I

G

G

G

A

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

R

M

R

Y

L

G

G

A

A

D

E

V

V

T

V

L

V

T

F

E

E

L

A

K

M

D

D

R

A

L

F

L

L

P

P

M

M

A

A

G

-

I

-

P

D

E

K

E

A

S

L

S

S

R

K

F

E

L

Y

A

Q

R

K

K

I

L

L

K

T

K

K

L

Q

G

G

I

L

D

D

I

I

K

R

L

I

K

G

T

A

T

K

V

V

N

S

H

G

Y

T

E

E

K

V

T

N

E

G

E

R

G

E

L

V

K

T

V

V

S

K

F

Y

T

T

D

Q

G

A

-

G

-

E

-

D

T

K

Q

E

L

Q

T

T

T

F

D

D

I

K

I

L

L

I

L

V

A

C

V

V

G

G

R
|
R

K

K

P

A

N
x
Y

T

A

E

E

G

G

F

L

-

L

-

A

K

E

E

D

T

S

G

G

I

I

E

K

M

L

D

T

E

E

R

R

G

G

F

L

V

V

V

E

V

V

D

N

K

D

H

H

C

C

R

A

T

T

N

S

L

V

P

E

S

G

V

V

Y

Y

A

A

C

I

G

G

D
|
D

I

L

T

V

S

R

P

G

L

P

M
|
M

L
|
L

A
|
A

H

H

K

K

A
|
A

M

M

Y

E

E

E

G

G

K

V

V

M

A

A

V

V

S

E

H

R

M

I

L

H

G

G

D

H

E

A

G

A

W

Q

E

V

K

N

N

Y

D

D

T

T

L

I

M

I

P

-

K

S

I

I

Y
|
Y

S

T

A

H

Y

P

E

E

V

A

A

A

S

W

V

V

G

G

L

L

T

T

E

E

D

E

E

Q

A

A

E

K

D

E

E

K

G

G

Y

H

H

E

V

V

R

K

V

T

G

G

V

Q

V

F

S

G

F

F

V

A

T

V

N

N

P

G

K

R

A

A

M

L

D

A

G

G

E

E

N

G

E

A

G

G

F

F

V

V

R

K

V

F

V

V

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

L

L

L

G

G

C

M

H

H

I

V

L

I

G

G

P

P

N

A

A

A

G

S

E

D

L

I

L

V

H

H

Q

Q

V

G

I

M

H

I

A

A

M

L

K

E

A

F

G

V

L

S

G

S

A

V

D

E

F

D

M

L

S

Q

R

L

S

M

V

T

Y

F

S

G

H
|
H

P

P

S

T

L

F

S

S

E
|
E

A

V

I

V

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

active site: C45 (= C42), C50 (= C47), S53 (= S50), V187 (≠ A180), E191 (= E184), H447 (= H438), E452 (= E443)
binding flavin-adenine dinucleotide: I7 (≠ V8), G10 (= G11), P11 (≠ S12), G12 (= G13), E31 (= E32), K32 (≠ L33), R33 (vs. gap), G43 (= G40), T44 (≠ N41), C45 (= C42), G49 (= G46), C50 (= C47), K54 (= K51), T117 (≠ K113), G118 (= G114), S147 (≠ T140), G148 (= G141), S167 (≠ T160), I188 (≠ V181), R275 (= R267), Y278 (≠ N270), D315 (= D305), M321 (= M311), L322 (= L312), A323 (= A313), A326 (= A316), Y354 (= Y345)

2yquB Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 98% coverage: 3:457/463 of query aligns to 2:453/455 of 2yquB

query
sites
2yquB

F

Y

D

D

L

L

V

L

I

V

V

I

G
|
G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

M

K

K

V

V

A

G

F

V

V

V

E
|
E

L

-

S
x
K

P

E

E

K

T

A

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N
x
R

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

-

Y

-

G

-

A

L

Y

E

M

T

I

T

E

E

R

R

F

I

S

Y

H

E

M

A

S

K

R

K

-

G

-

L

Y

L

G

G

I

A

L

K

P

V

K

K

G
|
G

F
x
V

H

E

L

L

S

D

M

L

P

P

H

A

L

L

K

M

E

A

G

H

R

K

D

D

N

K

V

V

R

Q

T

A

I

N

R

T

E

Q

N

G

F

V

K

E

K

F

F

L

A

F

Q

K

H

K

L

N

G

G

I

I

P

A

I

R

F

H

Y

Q

G

G

K
x
T

G
x
A

F

R

L

F

K

L

D

S

P

E

N

R

T

K

V

V

V

L

V

V

E

E

G

E

A

T

D

G

I

E

T

E

L

L

R

E

S

A

R

R

F

Y

V

I

L

L

L

I

A

A

T
|
T

G
|
G

S

S

S

A

T

P

V

L

S

I

V

P

G

P

G

W

M

A

V

Q

A

V

D

D

G

Y

R

E

Y

R

I

V

H

V

D

T

T

S

D

T

T

E

I

A

W

L

N

S

L

F

E

P

E

E

F

V

P

P

K

K

R

R

V

L

V

I

I

V

V

V

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

V

I

V

F

W

R

H

M

R

Y

L

G

G

A

A

D

E

V

V

T

I

L

V

T

L

E

E

L

Y

K

M

D

D

R

R

L

I

L

L

P

P

M

T

A

M

G

D

I

L

P

-

E

-

E

E

S

V

S

S

R

R

F

A

L

A

A

E

R

R

K

V

L

F

K

K

L

Q

G

G

I

L

D

T

I

I

K

R

L

T

K

G

T

V

T

R

V

V

N

T

H

A

Y

V

E

V

K

P

T

E

E

A

E

K

G

G

L

A

K

R

V

V

S

E

F

L

T

E

D

G

G

G

T

E

Q

V

L

L

T

E

T

A

D

D

I

R

I

V

L

L

L

V

A

A

V

V

G

G

R

R

K

R

P

P

N
x
Y

T

T

E

E

G

G

F

L

-

S

-

L

K

E

E

N

T

A

G

G

I

L

E

S

M

T

D

D

E

E

R

R

G

G

F

R

V

I

V

P

V

V

D

D

K

E

H

H

C

L

R

R

T

T

N

R

L

V

P

P

S

H

V

I

Y

Y

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

R

P

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M
x
S

Y

E

E

E

G

G

K

I

V

A

A

A

V

V

S

E

H

H

M

M

L

V

G

-

D

-

E

R

G

G

W

F

E

G

K

H

N

V

D

D

-

Y

T

Q

L

A

M

I

P

P

K

S

I

V

Y

Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

A

V

V

G

G

L

Y

T

T

E

E

D

E

E

E

A

L

E

K

D

A

E

Q

G

G

Y

I

H

P

V

Y

R

K

V

V

G

G

V

K

V

F

S

P

F

Y

V

S

T

A

N

S

P

G

K

R

A

A

M

R

D

A

D

M

G

G

E

E

N

T

E

E

G

G

F

F

V

I

R

K

V

V

V

L

V

A

E

H

E

A

R

K

T

T

K

D

R

R

I

I

L

L

G

G

C

V

H

H

I

G

L

I

G

G

P

A

N

R

A

V

G

G

E

D

L

V

L

L

H

A

Q

E

V

A

I

A

H

L

A

A

M

L

K

F

A

F

G

K

L

A

G
x
S

A

A

D

E

F
x
D

M

L

S

G

R

R

S

A

V

P

Y
x
H

S

A

H
|
H

P

P

S

S

L

L

S

S

E
|
E

A

I

I

L

G

K

Q

E

A

A

S

A

M

L

E

A

V

A

H

W

F

E

G

R

P

P

I

I

active site: P11 (≠ S12), L36 (≠ V38), C40 (= C42), C45 (= C47), S48 (= S50), G72 (= G75), V73 (≠ F76), V177 (≠ A180), E181 (= E184), S314 (≠ M317), H432 (≠ Y436), H434 (= H438), E439 (= E443)
binding carbonate ion: A310 (= A313), S314 (≠ M317), S423 (≠ G427), D426 (≠ F430)
binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), P11 (≠ S12), G12 (= G13), E31 (= E32), K32 (≠ S34), G38 (= G40), T39 (≠ N41), C40 (= C42), R42 (≠ N44), G44 (= G46), C45 (= C47), K49 (= K51), T110 (≠ K113), A111 (≠ G114), T137 (= T140), G138 (= G141), I178 (≠ V181), Y265 (≠ N270), G301 (= G304), D302 (= D305), M308 (= M311), L309 (= L312), A310 (= A313), H311 (= H314)

2yquA Crystal structures and evolutionary relationship of two different lipoamide dehydrogenase(e3s) from thermus thermophilus
35% identity, 98% coverage: 3:457/463 of query aligns to 2:453/455 of 2yquA

query
sites
2yquA

F

Y

D

D

L

L

V

L

I

V

V

I

G
|
G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

M

K

K

V

V

A

G

F

V

V

V

E
|
E

L

-

S
x
K

P

E

E

K

T

A

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N
x
R

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

-

Y

-

G

-

A

L

Y

E

M

T

I

T

E

E

R

R

F

I

S

Y

H

E

M

A

S

K

R

K

-

G

-

L

Y

L

G

G

I

A

L

K

P

V

K

K

G
|
G

F
x
V

H

E

L

L

S

D

M

L

P

P

H

A

L

L

K

M

E

A

G

H

R

K

D

D

N

K

V

V

R

Q

T

A

I

N

R

T

E

Q

N

G

F

V

K

E

K

F

F

L

A

F

Q

K

H

K

L

N

G

G

I

I

P

A

I

R

F

H

Y

Q

G

G

K
x
T

G
x
A

F

R

L

F

K

L

D

S

P

E

N

R

T

K

V

V

V

L

V

V

E

E

G

E

A

T

D

G

I

E

T

E

L

L

R

E

S

A

R

R

F

Y

V

I

L

L

L

I

A

A

T
|
T

G
|
G

S

S

S

A

T

P

V

L

S

I

V

P

G

P

G

W

M

A

V

Q

A

V

D

D

G

Y

R

E

Y

R

I

V

H

V

D

T

T
x
S

D

T

T

E

I

A

W

L

N

S

L

F

E

P

E

E

F

V

P

P

K

K

R

R

V

L

V

I

I

V

V

V

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

V

I

V

F

W

R

H

M

R

Y

L

G

G

A

A

D

E

V

V

T

I

L

V

T

L

E

E

L

Y

K

M

D

D

R

R

L

I

L

L

P

P

M

T

A

M

G

D

I

L

P

-

E

-

E

E

S

V

S

S

R

R

F

A

L

A

A

E

R

R

K

V

L

F

K

K

L

Q

G

G

I

L

D

T

I

I

K

R

L

T

K

G

T

V

T

R

V

V

N

T

H

A

Y

V

E

V

K

P

T

E

E

A

E

K

G

G

L

A

K

R

V

V

S

E

F

L

T

E

D

G

G

G

T

E

Q

V

L

L

T

E

T

A

D

D

I

R

I

V

L

L

L

V

A

A

V

V

G

G

R

R

K

R

P

P

N
x
Y

T

T

E

E

G

G

F

L

-

S

-

L

K

E

E

N

T

A

G

G

I

L

E

S

M

T

D

D

E

E

R

R

G

G

F

R

V

I

V

P

V

V

D

D

K

E

H

H

C

L

R

R

T

T

N

R

L

V

P

P

S

H

V

I

Y

Y

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

R

P

G

L

P

M
|
M

L
|
L

A
|
A

H

H

K

K

A

A

M
x
S

Y

E

E

E

G

G

K

I

V

A

A

A

V

V

S

E

H

H

M

M

L

V

G

-

D

-

E

R

G

G

W

F

E

G

K

H

N

V

D

D

-

Y

T

Q

L

A

M

I

P

P

K

S

I

V

Y

Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

A

V

V

G

G

L

Y

T

T

E

E

D

E

E

E

A

L

E

K

D

A

E

Q

G

G

Y

I

H

P

V

Y

R

K

V

V

G

G

V

K

V

F

S

P

F

Y

V

S

T

A

N

S

P

G

K

R

A

A

M

R

D

A

D

M

G

G

E

E

N

T

E

E

G

G

F

F

V

I

R

K

V

V

V

L

V

A

E

H

E

A

R

K

T

T

K

D

R

R

I

I

L

L

G

G

C

V

H

H

I

G

L

I

G

G

P

A

N

R

A

V

G

G

E

D

L

V

L

L

H

A

Q

E

V

A

I

A

H

L

A

A

M

L

K

F

A

F

G

K

L

A

G

S

A

A

D

E

F

D

M

L

S

G

R

R

S

A

V

P

Y
x
H

S

A

H
|
H

P

P

S

S

L

L

S

S

E
|
E

A

I

I

L

G

K

Q

E

A

A

S

A

M

L

E

A

V

A

H

W

F

E

G

R

P

P

I

I

active site: P11 (≠ S12), L36 (≠ V38), C40 (= C42), C45 (= C47), S48 (= S50), G72 (= G75), V73 (≠ F76), V177 (≠ A180), E181 (= E184), S314 (≠ M317), H432 (≠ Y436), H434 (= H438), E439 (= E443)
binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), P11 (≠ S12), G12 (= G13), E31 (= E32), K32 (≠ S34), G38 (= G40), T39 (≠ N41), C40 (= C42), R42 (≠ N44), G44 (= G46), C45 (= C47), K49 (= K51), T110 (≠ K113), A111 (≠ G114), T137 (= T140), G138 (= G141), S157 (≠ T160), I178 (≠ V181), Y265 (≠ N270), G301 (= G304), D302 (= D305), M308 (= M311), L309 (= L312), A310 (= A313)

2eq6A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8
36% identity, 97% coverage: 3:453/463 of query aligns to 4:452/460 of 2eq6A

query
sites
2eq6A

F

Y

D

D

L

L

V

I

I

V

V
x
I

G
|
G

A

T

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

H

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

L

K

K

V

V

A

L

F

A

V

V

E
|
E

L
x
A

S

G

P

-

E

-

T

E

V
|
V

G

G

G
|
G

N
x
V

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
x
T

K
|
K

Y

A

M

L

R

L

Y

H

G

A

A

A

Y

E

M

T

I

L

E

H

R

H

F

L

S

K

H

V

M

A

S

E

R

G

Y

F

G

G

I

L

L

K

P

A

K

K

G

-

F

P

H

E

L

L

S

D

M

L

P

K

H

K

L

L

K

G

E

G

G

W

R

R

D

D

N

Q

V

V

R

K

T

K

I

L

R

T

E

G

N

G

F

V

K

G

K

T

F

L

A

L

Q

K

H

G

L

N

G

G

I

V

P

E

I

L

F

L

Y

R

G

G

K
x
F

G
x
A

F

R

L

L

K

V

D

G

P

P

N

K

T

E

V

V

V

E

V

V

E

G

G

G

A

E

D

R

I

Y

T

G

L

A

R

K

S

S

R

-

F

-

V

L

L

I

L

L

A
|
A

T
|
T

G
|
G

S

S

S

E

T

P

V

L

S

E

V

L

G

K

G

G

M

F

V

-

A

P

D

F

G

G

R

E

Y

D

I

V

H

W

D

D

T
x
S

D

T

T

R

I

A

W

L

N

K

L

V

E

E

-

G

F

L

P

P

K

K

R

R

V

L

I

V

V

I

G

G

A
|
A

V

V

G

G

V

L

E
|
E

F

L

A

G

Y

Q

I

V

F

Y

R

R

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

L

T

I

E

E

L

Y

K

M

D

P

R

E

L

I

L

L

P

P

M

Q

A

-

G

G

I

D

P

P

E

E

E

-

S

T

S

A

R

A

F

L

L

L

A

R

R

R

K

A

L

L

K

E

K

K

L

E

G

G

I

I

D

R

I

V

K

R

L

T

K

K

T

T

T

K

V

A

N

V

H

G

Y

Y

E

E

K

K

T

K

E

K

E

D

G

G

L

L

K

H

V

V

S

R

F

L

T

E

D

P

G

A

T

E

Q

G

L

G

T

E

T

G

D

E

I

E

I

V

L

V

A

D

V

K

-

V

-

L

-

V

-

A

-

V

G

G

R

R

K

K

P

P

N
x
R

T

T

E

E

G

G

F

L

-

G

-

L

K

E

E

K

T

A

G

G

I

V

E

K

M

V

D

D

E

E

R

R

G

G

F

F

V

I

V

R

V

V

D

N

K

A

H

R

C

M

R

E

T

T

N

S

L

V

P

P

S

G

V

V

Y

Y

A

A

C

I

G

G

D
|
D

I

A

T

A

S

R

P

P

L

P

M
x
L

L
|
L

A
|
A

H
|
H

K

K

A

A

M

M

Y

R

E

E

G

G

K

L

V

I

A

A

V

A

S

E

H

N

M

A

L

A

G

G

D

K

E

D

G

-

W

-

E

S

K

A

N

F

D

D

T

Y

L

Q

M

V

P

P

K

S

I

V

Y
|
Y

S

T

A

S

Y

P

E

E

V

W

A

A

S

G

V

V

G

G

L

L

T

T

E

E

D

E

E

E

A

A

E

K

D

R

E

A

G

G

Y

Y

H

K

V

V

R

K

V

V

G

G

V

K

V

F

S

P

F

L

V

A

T

A

N

S

P

G

K

R

A

A

M

L

D

T

D

L

G

G

E

G

N

A

E

E

G

G

F

M

V

V

R

K

V

V

V

G

E

D

E

E

R

E

T

T

K

D

R

L

I

L

L

L

G

G

C

V

H

F

I

I

L

V

G

G

P

P

N

Q

A

A

G

G

E

E

L

L

L

I

H

A

Q

E

V

A

I

A

H

L

A

A

M

L

K

E

A

M

G

G

L

A

G

T

A

L

D

T

F

D

M

L

S

A

R

L

S

T

V

V

Y
x
H

S

P

H
|
H

P

P

S

T

L

L

S

S

E
|
E

A

S

I

L

G

M

Q

E

A

A

S

A

M

E

E

A

V

F

H

H

active site: V37 (= V38), C41 (= C42), C46 (= C47), T49 (≠ S50), A176 (= A180), E180 (= E184), H435 (≠ Y436), H437 (= H438), E442 (= E443)
binding flavin-adenine dinucleotide: I9 (≠ V8), G10 (= G9), G12 (= G11), P13 (≠ S12), G14 (= G13), E33 (= E32), A34 (≠ L33), G39 (= G40), V40 (≠ N41), C41 (= C42), G45 (= G46), C46 (= C47), K50 (= K51), F111 (≠ K113), A112 (≠ G114), A135 (= A139), T136 (= T140), G137 (= G141), S155 (≠ T160), R269 (≠ N270), D306 (= D305), L312 (≠ M311), L313 (= L312), A314 (= A313), H315 (= H314), Y344 (= Y345)

Sites not aligning to the query:

active site: 460

2eq7A Crystal structure of lipoamide dehydrogenase from thermus thermophilus hb8 with psbdo
35% identity, 97% coverage: 3:450/463 of query aligns to 2:446/452 of 2eq7A

query
sites
2eq7A

F

Y

D

D

L

L

V

L

I

V

V

I

G

G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

M

K

K

V

V

A

G

F

V

V

V

E
|
E

L

-

S
x
K

P

E

E

K

T

A

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N
x
R

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

-

Y

-

G

-

A

L

Y

E

M

T

I

T

E

E

R

R

F

I

S

Y

H

E

M

A

S

K

R

K

-

G

-

L

Y

L

G

G

I

A

L

K

P

V

K

K

G
|
G

F
x
V

H

E

L

L

S

D

M

L

P

P

H

A

L

L

K

M

E

A

G

H

R

K

D

D

N

K

V

V

R

Q

T

A

I

N

R

T

E

Q

N

G

F

V

K

E

K

F

F

L

A

F

Q

K

H

K

L

N

G

G

I

I

P

A

I

R

F

H

Y

Q

G

G

K
x
T

G
x
A

F

R

L

F

K

L

D

S

P

E

N

R

T

K

V

V

V

L

V

V

E

E

G

E

A

T

D

G

I

E

T

E

L

L

R

E

S

A

R

R

F

Y

V

I

L

L

L

I

A

A

T
|
T

G
|
G

S

S

S

A

T

P

V

L

S

I

V

P

G

P

G
x
W

M

A

V

Q

A

V

D

D

G

Y

R

E

Y

R

I

V

H

V

D

T

T
x
S

D

T

T

E

I

A

W

L

N

S

L

F

E

P

E

E

F

V

P

P

K

K

R

R

V

L

V

I

I

V

V

V

G
|
G

G

G

G
|
G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

V

I

V

F

W

R

H

M

R

Y

L

G

G

A

A

D

E

V

V

T

I

L

V

T

L

E
|
E

L
x
Y

K

M

D

D

R

R

L

I

L

L

P

P

M

T

A

M

G

D

I

L

P

-

E

-

E

E

S

V

S

S

R

R

F

A

L

A

A

E

R

R

K

V

L

F

K

K

L

Q

G

G

I

L

D

T

I

I

K

R

L

T

K

G

T

V

T

R

V
|
V

N

T

H

A

Y

V

E

V

K

P

T

E

E

A

E

K

G

G

L

A

K

R

V

V

S

E

F

L

T

E

D

G

G

G

T

E

Q

V

L

L

T

E

T

A

D

D

I

R

I

V

L

L

L

V

A

A

V
|
V

G
|
G

R
|
R

K

R

P

P

N
x
Y

T

T

E

E

G

G

F

L

-

S

-

L

K

E

E

N

T

A

G

G

I

L

E

S

M

T

D

D

E

E

R

R

G

G

F

R

V

I

V

P

V

V

D

D

K

E

H

H

C

L

R

R

T

T

N

R

L

V

P

P

S

H

V

I

Y

Y

A

A

C

I

G

G

D
|
D

I

V

T

V

S

R

P

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M
x
S

Y

E

E

E

G

G

K

I

V

A

A

A

V

V

S

E

H

H

M

M

L

V

G

-

D

-

E

R

G

G

W

F

E

G

K

H

N

V

D

D

-

Y

T

Q

L

A

M

I

P

P

K

S

I
x
V

I

V

Y
|
Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

A

V

V

G

G

L

Y

T

T

E

E

D

E

E

E

A

L

E

K

D

A

E

Q

G

G

Y

I

H

P

V

Y

R

K

V

V

G

G

V

K

V

F

S

P

F

Y

V

S

T

A

N

S

P

G

K

R

A

A

M

R

D

A

D

M

G

G

E

E

N

T

E

E

G

G

F

F

V

I

R

K

V

V

V

L

V

A

E

H

E

A

R

K

T

T

K

D

R

R

I

I

L

L

G

G

C

V

H

H

I

G

L

I

G

G

P

A

N

R

A

V

G

G

E

D

L

V

L

L

H

A

Q

E

V

A

I

A

H

L

A

A

M

L

K

F

A

F

G

K

L

A

G

S

A

A

D

E

F

D

M

L

S

G

R

R

S

A

V

P

Y
x
H

S

A

H
|
H

P

P

S

S

L

L

S

S

E
|
E

A

I

I

L

G

K

Q

E

A

A

S

A

M

L

active site: P11 (≠ S12), L36 (≠ V38), C40 (= C42), C45 (= C47), S48 (= S50), G72 (= G75), V73 (≠ F76), V177 (≠ A180), E181 (= E184), S314 (≠ M317), H432 (≠ Y436), H434 (= H438), E439 (= E443)
binding flavin-adenine dinucleotide: G10 (= G11), P11 (≠ S12), G12 (= G13), E31 (= E32), K32 (≠ S34), G38 (= G40), T39 (≠ N41), C40 (= C42), R42 (≠ N44), G44 (= G46), C45 (= C47), K49 (= K51), T110 (≠ K113), A111 (≠ G114), T137 (= T140), G138 (= G141), S157 (≠ T160), I178 (≠ V181), R262 (= R267), Y265 (≠ N270), D302 (= D305), M308 (= M311), L309 (= L312), A310 (= A313), H311 (= H314), Y341 (= Y345)
binding nicotinamide-adenine-dinucleotide: W146 (≠ G149), G174 (= G177), G176 (= G179), V177 (≠ A180), I178 (≠ V181), E197 (= E200), Y198 (≠ L201), V231 (= V236), V260 (= V265), G261 (= G266), R262 (= R267), M308 (= M311), L309 (= L312), V339 (≠ I343)

6awaA 1.83 angstrom resolution crystal structure of dihydrolipoyl dehydrogenase from pseudomonas putida in complex with fad and adenosine-5'-monophosphate.
33% identity, 100% coverage: 2:462/463 of query aligns to 4:475/475 of 6awaA

query
sites
6awaA

E

K

F

F

D

D

L

V

V

V

I

V

V
x
I

G

G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

K

A

A

H

A

R

Q

R

L

G

G

M

L

K

K

V

T

A

A

F

C

V

I

E
|
E

L
x
K

S

Y

P

T

E

D

T

A

-

E

-

G

-

K

-

L

-

A

V
x
L

G

G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

D

G

S

A

S

Y

W

M

K

I

Y

-

K

E

E

R

A

F

K

S

E

H

S

M

F

S

N

R

V

Y

H

G

G

I

I

L

S

P

T

K

G

G

E

F

V

H

K

L

M

S

D

M

V

P

A

H

A

L

M

K

V

E

G

G

R

R

K

D

A

N

G

V

I

V

V

R

K

T

N

I

L

R

T

E

G

N

G

F

V

K

A

K

T

F

L

A

F

Q

K

H

A

L

N

G

G

I

V

P

T

I

S

F

I

Y

Q

G

G

K
x
H

G
|
G

F

K

L

L

K

L

D

A

P

G

N

K

T

K

V

V

V

E

V

V

E

T

G

K

A

A

D

D

I

G

T

T

-

T

-

E

-

V

L

I

R

E

S

A

R

E

F

N

V

V

L

I

L

L

A

A

T
x
S

G
|
G

S

S

S

R

T

P

V

I

S

D

V

I

G

P

M

A

V

P

A

V

D

D

G

Q

R

N

Y

V

I

I

H

V

D

D

T

S

D

T

T

G

I

A

W

L

N

E

L

F

E

Q

E

A

F

V

P

P

K

K

R

R

V

L

V

G

I

V

V
x
I

G

G

G

A

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

A

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

V

T

L

E
|
E

L
x
A

K
x
L

D

D

R

T

L

F

L

L

P

M

M

A

A

A

G

-

I

-

P

D

E

T

E

A

S

V

S

S

R

K

F

E

L

A

A

Q

R

K

K

T

L

L

K

T

K

K

L

Q

G

G

I

L

D

D

I

I

K

K

L

L

K

G

T

A

T

R

V
|
V

N

T

H

G

Y

S

E

K

K

V

T

N

E

G

E

N

G

E

L

V

K

E

V

V

S

T

F

Y

T

T

D

N

G

A

-

E

-

G

-

E

T

Q

Q

K

L

I

T

T

T

F

D

D

I

K

I

L

L

I

L

V

A

A

V
|
V

G

G

R
|
R

K

R

P

P

N

V

T

T

E

T

G

D

F

L

-

L

-

A

K

S

E

D

T

S

G

G

I

V

E

T

M

I

D

D

E

E

R

R

G

G

F

Y

V

I

V

F

V

V

D

D

K

D

H

Y

C

C

R

A

T

T

N

S

L

V

P

P

S

G

V

V

Y

Y

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

R

P

G

L

M

M
|
M

L
|
L

A
|
A

H

H

K

K

A

A

M

S

Y

E

E

E

G

G

K

I

V

M

A

V

V

V

S

E

H

R

M

I

L

K

G

G

D

H

E

K

G

A

W

-

E

Q

K

M

N

N

D

Y

T

D

L

L

M

I

P

P

K

S

I

V

I

I

Y
|
Y

S

T

A

H

Y

P

E

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

D

Q

E

A

A

L

E

K

D

A

E

E

G

G

Y

V

H

E

V

V

R

N

V

V

G

G

V

T

V

F

S

P

F

F

V

A

T

A

N

S

P

G

K

R

A

A

M

M

D

A

G

N

E

D

N

T

E

G

G

G

F

F

V

V

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

R

I

V

L

L

G

G

C

V

H

H

I

V

L

I

G

G

P

P

N

S

A

A

G

A

E

E

L

L

L

V

H

Q

Q

Q

V

G

I

A

H

I

A

A

M

M

K

E

A

F

G

G

L

T

G

S

A

A

D

E

F

D

M

L

S

G

R

M

S

M

V

V

Y
x
F

S

S

H
|
H

P

P

S

T

L

L

S

S

E
|
E

A

A

I

L

G

H

Q

E

A

A

S

A

M

L

E

A

V

V

H

N

F

G

G

G

P

A

I

I

T

H

W

V

T

A

K
x
N

R
|
R

active site: L45 (≠ V38), C49 (= C42), C54 (= C47), S57 (= S50), V191 (≠ A180), E195 (= E184), F449 (≠ Y436), H451 (= H438), E456 (= E443), N474 (≠ K461), R475 (= R462)
binding adenosine monophosphate: I187 (≠ V176), E211 (= E200), A212 (≠ L201), L213 (≠ K202), V245 (= V236), V277 (= V265)
binding flavin-adenine dinucleotide: I10 (≠ V8), G13 (= G11), P14 (≠ S12), G15 (= G13), E34 (= E32), K35 (≠ L33), T48 (≠ N41), C49 (= C42), G53 (= G46), C54 (= C47), K58 (= K51), H121 (≠ K113), G122 (= G114), S151 (≠ T140), G152 (= G141), I192 (≠ V181), R279 (= R267), G318 (= G304), D319 (= D305), M325 (= M311), L326 (= L312), A327 (= A313), Y358 (= Y345)

P11959 Dihydrolipoyl dehydrogenase; Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex; EC 1.8.1.4 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
33% identity, 99% coverage: 1:457/463 of query aligns to 8:465/470 of P11959

query
sites
P11959

M

I

E

E

F

T

D

E

L

T

V

L

I

V

V

V

G

G

A

A

G

G

S

P

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

Q

K

K

V

V

A

T

F

I

V

V

E
|
E

L
x
K

S
x
G

P

-

E

-

T
x
N

V
x
L

G
|
G

N
x
V

C
|
C

L

L

N

N

R

V

G

G

C

C

I

I

P

P

S

S

K
|
K

Y

A

M

L

R

I

Y

S

G

A

A

S

Y

H

M

R

I

Y

E

E

R

Q

F

A

S

K

H

H

M

S

S

E

R

E

Y

M

G

G

I

I

L

K

P

A

K

E

G

N

F

V

H

T

L

I

S

D

M

F

P

A

H

K

L

V

K

Q

E

E

G

W

R

K

D

A

N

S

V

V

R

K

T

K

I

L

R

T

E

G

N

G

F

V

K

E

K

G

F

L

A

L

Q

K

H

G

L

N

G

K

I

V

P

E

I

I

F

V

Y

K

G

G

K

E

G

A

F

Y

L

F

K

V

D

D

P

A

N

N

T

T

V

V

V

R

V

V

E

V

G

N

A

G

D

D

I

S

T

A

L

Q

R

T

S

Y

R

T

F

F

-

K

-

N

V

A

L

I

A

A

T

T

G

G

S

S

S

R

T

P

V

I

S

E

V

L

G

P

G

N

M

F

V

K

A

F

D

S

G

N

R

R

Y

-

I

I

H

L

D

D

T

S

D

T

T

G

I

A

W

L

N

N

L

L

E

G

E

E

F

V

P

P

K

K

R

S

V

L

V

V

I

V

V

I

G

G

A

Y

V

I

G

G

V

I

E

E

F

L

A

G

Y

T

I

A

F

Y

R

A

M

N

Y

F

G

G

A

T

D

K

V

V

T

T

L

I

T

L

E

E

L

G

K

A

D

G

R

E

L

I

L

L

P

-

M

-

A

S

G

G

I

F

P

E

E

K

E

Q

S

M

S

A

R

A

F

I

L

I

A

K

R

K

K

R

L

L

K

K

L

K

G

G

I

V

D

E

I

V

K

V

L

T

K

N

T

A

T

L

V

A

N

K

H

G

Y

A

E

E

K

E

T

R

E

E

E

D

G

G

L

V

K

T

V

V

S

T

F

Y

T

E

D

A

G

N

T

G

Q

E

L

T

T

K

T

T

-

I

-

D

-

A

D

D

I

Y

I

V

L

L

L

V

A

T

V

V

G

G

R

R

K

R

P

P

N

N

T

T

E

D

-

E

-

L

G

G

F

L

K

E

E

Q

T

I

G

G

I

I

E

K

M

M

D

T

E

N

R

R

G

G

F

L

V

I

V

E

V

V

D

D

K

Q

H

Q

C

C

R

R

T

T

N

S

L

V

P

P

S

N

V

I

Y

F

A

A

C

I

G

G

D
|
D

I

I

T

V

S

P

P

G

L

P

M

A

L

L

A
|
A

H

H

K

K

A

A

M

S

Y

Y

E

E

G

G

K

K

V

V

A

A

V

A

S

E

H

A

M

I

L

A

G

G

D

H

E

P

G

S

W

A

E

-

K

V

N

D

D

Y

T

V

L

A

M

I

P

P

K

A

I

V

Y

F

S

S

A

D

Y

P

E

E

V

C

A

A

S

S

V

V

G

G

L

Y

T

F

E

E

D

Q

E

Q

A

A

E

K

D

D

E

E

G

G

Y

I

H

D

V

V

R

I

V

A

G

A

V

K

V

F

S

P

F

F

V

A

T

A

N

N

P

G

K

R

A

A

M

L

D

A

D

L

G

N

E

D

N

T

E

D

G

G

F

F

V

L

R

K

V

L

V

V

E

R

E

K

R

E

T

D

K

G

R

V

I

I

L

I

G

G

C

A

H

Q

I

I

L

I

G

G

P

P

N

N

A

A

G

S

E

D

L

M

L

I

H

A

Q

E

V

L

I

G

H

L

A

A

M

I

K

E

A

A

G

G

L

M

G

T

A

A

D

E

F

D

M

I

S

A

R

L

S

T

V

I

Y

H

S

A

H

H

P

P

S

T

L

L

S

G

E

E

A

-

I

I

G

A

Q

M

A

E

S

A

M

A

E

E

V

V

H

A

F

L

G

G

-

T

P

P

I

I

39:47 (vs. 32:42, 36% identical) binding FAD
K56 (= K51) binding FAD
D314 (= D305) binding FAD
A322 (= A313) binding FAD

6aonA 1.72 angstrom resolution crystal structure of 2-oxoglutarate dehydrogenase complex subunit dihydrolipoamide dehydrogenase from bordetella pertussis in complex with fad
32% identity, 97% coverage: 2:452/463 of query aligns to 2:466/473 of 6aonA

query
sites
6aonA

E

Q

F

F

D

D

L

V

V

V

I

V

V
x
I

G

G

A

A

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

I

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

M

K

S

V

V

A

A

F

C

V

I

E
x
D

L
x
A

S
x
W

P

Q

E

N

-

G

-

Q

-

G

-

P

T

A

V
x
P

G

G

G
|
G

N
x
T

C
|
C

L

T

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

Q

G

S

A

S

Y

E

M

H

I

Y

E

E

R

Q

F

A

S

N

H

H

-

H

M

F

S

A

R

E

Y

H

G

G

I

I

L

E

P

V

K

K

G

G

F

V

H

S

L

L

S

K

M

L

P

D

H

T

L

L

K

I

E

G

G

R

R

K

D

N

N

T

V

V

R

K

T

Q

I

N

R

N

E

D

N

G

F

I

K

L

K

Y

F

L

A

F

Q

K

H

K

L

N

G

K

I

V

P

T

I

Y

F

F

Y

H

G

G

K
|
K

G
|
G

F

A

L

F

K

A

D

G

P

Q

-

V

-

D

-

G

-

W

N

S

T

I

V

K

V

V

V

T

E

G

G

T

A

T

D

D

I

A

T

D

L

L

R

V

S

A

R

K

F

H

V

V

L

I

L

V

A

A

T
|
T

G
|
G

S

S

T

A

V

R

S

E

V

L

G

P

G

G

M

L

V

P

A

F

D

D

G

E

R

K

Y

N

I

I

H

L

D

S

T
x
N

D

D

T

G

I

A

W

L

N

N

L

I

E

G

E

A

F

V

P

P

K

K

R

K

V

L

V

G

I

V

V

I

G

G

G

A

G

G

A
x
V

V
x
I

G

G

V

L

E
|
E

F

M

A

G

Y

S

I

V

F

W

R

R

M

R

Y

L

G

G

A

A

D

E

V

V

T

T

L

I

T

L

E

E

L

A

K

M

D

P

R

E

L

F

L

L

P
x
A

M

A

A
|
A

G

-

I

-

P

D

E
x
Q

E

Q

S

V

S

A

R

K

F

E

L

A

A

L

R

K

K

S

L

F

K

A

K

K

L

Q

G

G

I

L

D

D

I

I

K

Q

L

T

K

G

T

V

T

K

V

I

N

G

H

E

Y

I

E

K

K

A

T

A

E

A

E

K

G

S

L

I

K

T

V

V

S

P

F

Y

T

V

D

D

G

A

-

K

-

G

-

A

-

E

T

Q

Q

K

L

L

T

V

D

D

I

K

I

L

L

I

L

V

A

S

V

I

G

G

R
|
R

K

V

P

P

N
x
Y

T

T

E

G

G

G

F

L

-

N

-

A

K

E

E

A

T

V

G

G

I

L

E

K

M

L

D

D

E

E

R

R

G

G

F

F

V

V

V

A

V

V

D

D

K

E

H

D

C

C

R

K

T

T

N

N

L

L

P

P

S

N

V

V

Y

W

A

A

C

V

G
|
G

D
|
D

I

V

T

V

S

R

P

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M

E

Y

E

E

E

G

G

K

-

V

V

A

A

V

V

S

A

H

E

M

R

L

I

G

A

D

G

E

Q

G

H

W

G

E

H

K

V

N

N

D

F

T

A

L

T

M

V

P

P

K

W

I

V

I

I

Y

Y

S

T

A

S

Y

P

E

E

V

I

A

A

S

W

V

V

G

G

L

K

T

T

E

E

D

Q

E

Q

A

L

E

K

D

A

E

E

G

G

Y

R

H

E

V

Y

R

K

V

A

G

G

V

S

V

F

S

P

F

F

V

M

T

A

N

N

P

G

K

R

A

A

M

R

D

A

D

L

G

G

E

D

N

T

E

T

G

G

F

F

V

A

R

K

V

V

V

I

V

A

E

D

E

A

R

K

T

T

K

D

R

E

I

V

L

L

G

G

C

V

H

H

I

I

L

I

G

G

P

P

N

M

A

A

G

S

E

E

L

L

L

I

H

S

Q

E

V

A

I

V

H

T

A

I

M

M

K

E

A

F

G

R

L

G

G

A

A

A

D

E

F

D

M

I

S

A

R

R

S

I

V

C

Y
x
H

S

A

H
|
H

P

P

S

T

L

L

S

S

E
|
E

A

A

I

V

G

K

Q

E

A

A

S

A

M

L

E

A

V

V

active site: P43 (≠ V38), C47 (= C42), C52 (= C47), S55 (= S50), V191 (≠ A180), E195 (= E184), H450 (≠ Y436), H452 (= H438), E457 (= E443)
binding calcium ion: A218 (≠ P207), A220 (= A209), Q222 (≠ E213)
binding flavin-adenine dinucleotide: I8 (≠ V8), G11 (= G11), P12 (≠ S12), G13 (= G13), D32 (≠ E32), A33 (≠ L33), W34 (≠ S34), G45 (= G40), T46 (≠ N41), C47 (= C42), G51 (= G46), C52 (= C47), K56 (= K51), K119 (= K113), G120 (= G114), T151 (= T140), G152 (= G141), N171 (≠ T160), I192 (≠ V181), R280 (= R267), Y283 (≠ N270), G319 (= G304), D320 (= D305), M326 (= M311), L327 (= L312), A328 (= A313), H329 (= H314)

1ebdA Dihydrolipoamide dehydrogenase complexed with the binding domain of the dihydrolipoamide acetylase (see paper)
32% identity, 97% coverage: 1:449/463 of query aligns to 2:451/455 of 1ebdA

query
sites
1ebdA

M

I

E

E

F

T

D

E

L

T

V

L

I

V

V

V

G
|
G

A

A

G
|
G

S
x
P

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

R

A

A

H

A

R

Q

R

L

G

G

M

Q

K

K

V

V

A

T

F

I

V
|
V

E
|
E

L
x
K

S

G

P

-

E

-

T

N

V
x
L

G

G

G
|
G

N
x
V

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

I

Y

S

G

A

A

S

Y

H

M

R

I

Y

E

E

R

Q

F

A

S

K

H

H

M

S

S

E

R

E

Y

M

G

G

I

I

L

K

P

A

K

E

G
x
N

F
x
V

H

T

L

I

S

D

M

F

P

A

H

K

L

V

K

Q

E

E

G

W

R

K

D

A

N

S

V

V

R

K

T

K

I

L

R

T

E

G

N

G

F

V

K

E

K

G

F

L

A

L

Q

K

H

G

L

N

G

K

I

V

P

E

I

I

F

V

Y

K

G

G

K
x
E

G
x
A

F

Y

L

F

K

V

D

D

P

A

N

N

T

T

V

V

V

R

V

V

E

V

G

N

A

G

D

D

I

S

T

A

L

Q

R

T

S

Y

R

T

F

F

-

K

-

N

V

A

L

I

A

A

T
|
T

G
|
G

S

S

S

R

T

P

V

I

S

E

V

L

G

P

G

N

M

F

V

K

A

F

D

S

G

N

R

R

Y

-

I

I

H

L

D

D

T

S

D

T

T

G

I

A

W

L

N

N

L

L

E

G

E

E

F

V

P

P

K

K

R

S

V

L

V

V

I

V

V

I

G

G

A
x
Y

V
x
I

G

G

V

I

E
|
E

F

L

A

G

Y

T

I

A

F

Y

R

A

M

N

Y

F

G

G

A

T

D

K

V

V

T

T

L

I

T

L

E

E

L

G

K

A

D

G

R

E

L

I

L

L

P

-

M

-

A

S

G

G

I

F

P

E

E

K

E

Q

S

M

S

A

R

A

F

I

L

I

A

K

R

K

K

R

L

L

K

K

L

K

G

G

I

V

D

E

I

V

K

V

L

T

K

N

T

A

T

L

V

A

N

K

H

G

Y

A

E

E

K

E

T

R

E

E

E

D

G

G

L

V

K

T

V

V

S

T

F

Y

T

E

D

A

G

N

T

G

Q

E

L

T

T

K

T

T

-

I

-

D

-

A

D

D

I

Y

I

V

L

L

L

V

A

T

V

V

G

G

R
|
R

K

R

P

P

N

N

T

T

E

D

-

E

-

L

G

G

F

L

K

E

E

Q

T

I

G

G

I

I

E

K

M

M

D

T

E

N

R

R

G

G

F

L

V

I

V

E

V

V

D

D

K

Q

H

Q

C

C

R

R

T

T

N

S

L

V

P

P

S

N

V

I

Y

F

A

A

C

I

G

G

D
|
D

I

I

T

V

S

P

P

G

L

P

M
x
A

L
|
L

A
|
A

H

H

K

K

A

A

M
x
S

Y

Y

E

E

G

G

K

K

V

V

A

A

V

A

S

E

H

A

M

I

L

A

G

G

D

H

E

P

G

S

W

A

E

-

K

V

N

D

D

Y

T

V

L

A

M

I

P

P

K

A

I

V

Y

F

S

S

A

D

Y

P

E

E

V

C

A

A

S

S

V

V

G

G

L

Y

T

F

E

E

D

Q

E

Q

A

A

E

K

D

D

E

E

G

G

Y

I

H

D

V

V

R

I

V

A

G

A

V

K

V

F

S

P

F

F

V

A

T

A

N

N

P

G

K

R

A

A

M

L

D

A

D

L

G

N

E

D

N

T

E

D

G

G

F

F

V

L

R

K

V

L

V

V

E

R

E

K

R

E

T

D

K

G

R

V

I

I

L

I

G

G

C

A

H

Q

I

I

L

I

G

G

P

P

N

N

A

A

G

S

E

D

L

M

L

I

H

A

Q

E

V

L

I

G

H

L

A

A

M

I

K

E

A

A

G

G

L

M

G

T

A

A

D

E

F

D

M

I

S

A

R

L

S

T

V

I

Y
x
H

S

A

H
|
H

P

P

S

T

L

L

S

G

E
|
E

A

I

I

A

G

M

Q

E

A

A

S

A

active site: P13 (≠ S12), L37 (≠ V38), C41 (= C42), C46 (= C47), S49 (= S50), N74 (≠ G75), V75 (≠ F76), Y180 (≠ A180), E184 (= E184), S320 (≠ M317), H438 (≠ Y436), H440 (= H438), E445 (= E443)
binding flavin-adenine dinucleotide: G10 (= G9), G12 (= G11), P13 (≠ S12), V32 (= V31), E33 (= E32), K34 (≠ L33), G39 (= G40), V40 (≠ N41), C41 (= C42), G45 (= G46), C46 (= C47), K50 (= K51), E112 (≠ K113), A113 (≠ G114), T141 (= T140), G142 (= G141), Y180 (≠ A180), I181 (≠ V181), R268 (= R267), D308 (= D305), A314 (≠ M311), L315 (= L312), A316 (= A313)

6hg8B Crystal structure of the r460g disease-causing mutant of the human dihydrolipoamide dehydrogenase.
32% identity, 98% coverage: 1:455/463 of query aligns to 13:477/482 of 6hg8B

query
sites
6hg8B

M

I

E

D

F

A

D

D

L

V

V

T

I

V

V
x
I

G

G

A

S

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

K

A

A

H

A

R

Q

R

L

G

G

M

F

K

K

V

T

A

V

F

C

V

I

E
|
E

L
x
K

S
x
N

P

-

E

E

T

T

V

L

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

N

G

N

A

S

-

H

-

Y

Y

Y

M

H

I

M

E

A

R

H

F

G

S

K

H

D

M

F

S

A

R

S

Y

R

G

G

I

I

L

E

P

M

K

S

G

E

F

V

H

R

L

L

S

N

M

L

P

D

H

K

L

M

K

M

E

E

G

Q

R

K

D

S

N

T

V

A

V

V

R

K

T

A

I

L

R

T

E

G

N

G

F

I

K

A

K

H

F

L

A

F

Q

K

H

Q

L

N

G

K

I

V

P

V

I

H

F

V

Y

N

G

G

K
x
Y

G
|
G

F

K

L

I

K

T

D

G

P

K

N

N

T

Q

V

V

V

T

V

A

E

T

G

K

A

A

D

D

-

G

-

T

I

Q

T

V

L

I

R

D

S

T

R

K

F

N

V

I

L

L

L

I

A

A

T
|
T

G
|
G

S

S

S

E

T

V

V

T

S

P

V

F

G

P

G

G

M

I

V

T

A

I

D

D

G

E

R

D

Y

T

I

I

H

V

D

S

T

S

D

T

T

G

I

A

W

L

N

S

L

L

E

K

F

V

P

P

K

E

R

K

V

M

V

V

I

V

V

I

G

G

G

A

G

G

A
x
V

V
x
I

G

G

V

V

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

Q

M

R

Y

L

G

G

A

A

D

D

V

V

T

T

L

A

T

V

E

E

L

F

K

L

D

G

R

H

L

V

L

G

P

G

M

V

A

-

G

G

I

I

P

D

E

M

E

E

S

I

S

S

R

K

F

N

L

F

A

Q

R

R

K

I

L

L

K

Q

K

K

L

Q

G

G

I

F

D

K

I

F

K

K

L

L

K

N

T

T

T

K

V

V

N

T

H

G

Y

A

E

T

K

K

T

K

E

S

E

D

G

G

-

K

-

I

-

D

L

V

K

S

V

I

S

E

F

A

T

A

D

S

G

G

T

G

Q

K

-

A

-

E

-

V

L

I

T

T

T

C

D

D

I

V

I

L

L

L

L

V

A

C

V

I

G

G

R
|
R

K

R

P

P

N
x
F

T

T

E

K

-

N

-

L

G

G

F

L

K

E

E

E

T

L

G

G

I

I

E

E

M

L

D

D

E

P

R

R

G

G

F

R

V

I

V

P

V

V

D

N

K

T

H

R

C

F

R

Q

T

T

N

K

L

I

P

P

S

N

V

I

Y

Y

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

A

P

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M

E

Y

D

E

E

G

G

K

I

V

I

A

C

V

V

S

E

H

G

M

M

L

A

G

G

D

G

E

-

G

A

W

V

E

H

K

I

N

D

D

Y

T

N

L

C

M

V

P

P

K

S

I

V

I

I

Y

Y

S

T

A

H

Y

P

E

E

V

V

A

A

S

W

V

V

G

G

L

K

T

S

E

E

D

E

E

Q

A

L

E

K

D

E

E

E

G

G

Y

I

H

E

V

Y

R

K

V

V

G

G

V

K

V

F

S

P

F

F

V

A

T

A

N

N

P

S

K

R

A

A

M

K

D

T

D

N

G

A

E

D

N

T

E

D

G

G

F

M

V

V

R

K

V

I

V

L

V

G

E

Q

E

K

R

S

T

T

K

D

R

R

I

V

L

L

G

G

C

A

H

H

I

I

L

L

G

G

P

P

N

G

A

A

G

G

E

E

L

M

L

V

H

N

Q

E

V

A

I

A

H

L

A

A

M

L

K

E

A

Y

G

G

L

A

G

S

A

C

D

E

F

D

M

I

S

A

R

R

S

V

V

C

Y

H

S

A

H
|
H

P

P

S

T

L

L

S

S

E
|
E

A

A

I

F

G

G

Q

E

A

A

S

N

M

L

E

A

V

A

H

S

F

F

G

G

active site: C53 (= C42), C58 (= C47), S61 (= S50), V196 (≠ A180), E200 (= E184), H460 (= H438), E465 (= E443)
binding flavin-adenine dinucleotide: I20 (≠ V8), G23 (= G11), P24 (≠ S12), G25 (= G13), E44 (= E32), K45 (≠ L33), N46 (≠ S34), G51 (= G40), T52 (≠ N41), C53 (= C42), G57 (= G46), C58 (= C47), K62 (= K51), Y126 (≠ K113), G127 (= G114), T156 (= T140), G157 (= G141), I197 (≠ V181), R288 (= R267), F291 (≠ N270), G327 (= G304), D328 (= D305), M334 (= M311), L335 (= L312), A336 (= A313), H337 (= H314)

1zmdA Crystal structure of human dihydrolipoamide dehydrogenase complexed to nadh (see paper)
32% identity, 98% coverage: 1:455/463 of query aligns to 3:467/472 of 1zmdA

query
sites
1zmdA

M

I

E

D

F

A

D

D

L

V

V

T

I

V

V
x
I

G
|
G

A

S

G
|
G

S
x
P

G
|
G

G

G

Y

Y

E

V

A

A

G

A

L

I

Y

K

A

A

H

A

R

Q

R

L

G

G

M

F

K

K

V

T

A

V

F

C

V

I

E
|
E

L
x
K

S
x
N

P

-

E

E

T

T

V
x
L

G

G

G
|
G

N
x
T

C
|
C

L

L

N

N

R

V

G
|
G

C
|
C

I

I

P

P

S
|
S

K
|
K

Y

A

M

L

R

L

Y

N

G

N

A

S

-

H

-

Y

Y

Y

M

H

I

M

E

A

R

H

F

G

S

T

H

D

M

F

S

A

R

S

Y

R

G

G

I

I

L

E

P

M

K

S

G

E

F

V

H

R

L

L

S

N

M

L

P

D

H

K

L

M

K

M

E

E

G

Q

R

K

D

S

N

T

V

A

V

V

R

K

T

A

I

L

R

T

E

G

N

G

F

I

K

A

K

H

F

L

A

F

Q

K

H

Q

L

N

G

K

I

V

P

V

I

H

F

V

Y

N

G

G

K
x
Y

G
|
G

F

K

L

I

K

T

D

G

P

K

N

N

T

Q

V

V

V

T

V

A

E

T

G

K

A

A

D

D

-

G

-

T

I

Q

T

V

L

I

R

D

S

T

R

K

F

N

V

I

L

L

L

I

A

A

T
|
T

G
|
G

S

S

S

E

T

V

V

T

S

P

V

F

G

P

G

G

M

I

V

T

A

I

D

D

G

E

R

D

Y

T

I

I

H

V

D

S

T
x
S

D

T

T

G

I

A

W

L

N

S

L

L

E

K

F

V

P

P

K

E

R

K

V

M

V

V

I

V

V
x
I

G
|
G

G

A

G
|
G

A
x
V

V
x
I

G

G

V

V

E
|
E

F

L

A

G

Y

S

I

V

F

W

R

Q

M

R

Y

L

G

G

A

A

D

D

V

V

T

T

L

A

T

V

E
|
E

L
x
F

K
x
L

D

G

R

H

L

V

L

G

P

G

M

V

A

-

G

G

I

I

P

D

E

M

E

E

S

I

S

S

R

K

F

N

L

F

A

Q

R

R

K

I

L

L

K

Q

K

K

L

Q

G

G

I

F

D

K

I

F

K

K

L

L

K

N

T

T

T

K

V

V

N

T

H

G

Y

A

E

T

K

K

T

K

E

S

E

D

G

G

-

K

-

I

-

D

L

V

K

S

V

I

S

E

F

A

T

A

D

S

G

G

T

G

Q

K

-

A

-

E

-

V

L

I

T

T

T

C

D

D

I

V

I

L

L

L

L

V

A

C

V
x
I

G
|
G

R
|
R

K

R

P

P

N
x
F

T

T

E

K

-

N

-

L

G

G

F

L

K

E

E

E

T

L

G

G

I

I

E

E

M

L

D

D

E

P

R

R

G

G

F

R

V

I

V

P

V

V

D

N

K

T

H

R

C

F

R

Q

T

T

N

K

L

I

P

P

S

N

V

I

Y

Y

A

A

C

I

G
|
G

D
|
D

I

V

T

V

S

A

P

G

L

P

M
|
M

L
|
L

A
|
A

H
|
H

K

K

A

A

M

E

Y

D

E

E

G

G

K

I

V

I

A

C

V

V

S

E

H

G

M

M

L

A

G

G

D

G

E

-

G

A

W

V

E

H

K

I

N

D

D

Y

T

N

L

C

M

V

P

P

K

S

I
x
V

I

I

Y
|
Y

S

T

A

H

Y

P

E

E

V

V

A

A

S

W

V

V

G

G

L

K

T

S

E

E

D

E

E

Q

A

L

E

K

D

E

E

E

G

G

Y

I

H

E

V

Y

R

K

V

V

G

G

V

K

V

F

S

P

F

F

V

A

T

A

N

N

P

S

K

R

A

A

M

K

D

T

D

N

G

A

E

D

N

T

E

D

G

G

F

M

V

V

R

K

V

I

V

L

V

G

E

Q

E

K

R

S

T

T

K

D

R

R

I

V

L

L

G

G

C

A

H

H

I

I

L

L

G

G

P

P

N

G

A

A

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G

E

E

L

M

L

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Q

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A

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A

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A

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active site: L39 (≠ V38), C43 (= C42), C48 (= C47), S51 (= S50), V186 (≠ A180), E190 (= E184), H448 (≠ Y436), H450 (= H438), E455 (= E443)
binding flavin-adenine dinucleotide: I10 (≠ V8), G11 (= G9), G13 (= G11), P14 (≠ S12), G15 (= G13), E34 (= E32), K35 (≠ L33), N36 (≠ S34), G41 (= G40), T42 (≠ N41), C43 (= C42), G47 (= G46), C48 (= C47), K52 (= K51), Y116 (≠ K113), G117 (= G114), T146 (= T140), G147 (= G141), S166 (≠ T160), R278 (= R267), F281 (≠ N270), G317 (= G304), D318 (= D305), M324 (= M311), L325 (= L312), A326 (= A313), H327 (= H314)
binding 1,4-dihydronicotinamide adenine dinucleotide: I182 (≠ V176), G183 (= G177), G185 (= G179), V186 (≠ A180), I187 (≠ V181), E190 (= E184), E206 (= E200), F207 (≠ L201), L208 (≠ K202), I276 (≠ V265), G277 (= G266), R278 (= R267), M324 (= M311), L325 (= L312), V355 (≠ I343), Y357 (= Y345)

1zmcA Crystal structure of human dihydrolipoamide dehydrogenase complexed to NAD+ (see paper)
32% identity, 98% coverage: 1:455/463 of query aligns to 3:467/472 of 1zmcA

query
sites
1zmcA

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active site: L39 (≠ V38), C43 (= C42), C48 (= C47), S51 (= S50), V186 (≠ A180), E190 (= E184), H448 (≠ Y436), H450 (= H438), E455 (= E443)
binding flavin-adenine dinucleotide: I10 (≠ V8), G11 (= G9), G13 (= G11), P14 (≠ S12), G15 (= G13), E34 (= E32), K35 (≠ L33), N36 (≠ S34), G41 (= G40), T42 (≠ N41), C43 (= C42), G47 (= G46), C48 (= C47), K52 (= K51), Y116 (≠ K113), G117 (= G114), T146 (= T140), G147 (= G141), S166 (≠ T160), I187 (≠ V181), F281 (≠ N270), G317 (= G304), D318 (= D305), M324 (= M311), L325 (= L312), A326 (= A313), H327 (= H314)
binding nicotinamide-adenine-dinucleotide: G183 (= G177), G185 (= G179), V205 (≠ T199), E206 (= E200), F207 (≠ L201), L208 (≠ K202), K240 (≠ T235), V241 (= V236), I276 (≠ V265), G277 (= G266), R278 (= R267), R297 (= R284), M324 (= M311)

P31023 Dihydrolipoyl dehydrogenase, mitochondrial; Dihydrolipoamide dehydrogenase; Glycine cleavage system L protein; Pyruvate dehydrogenase complex E3 subunit; E3; PDC-E3; EC 1.8.1.4 from Pisum sativum (Garden pea) (Lathyrus oleraceus) (see 2 papers)
31% identity, 98% coverage: 2:457/463 of query aligns to 37:499/501 of P31023

query
sites
P31023

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67:76 (vs. 32:42, 36% identical) binding FAD
C76 (= C42) modified: Disulfide link with 81, Redox-active
C81 (= C47) modified: Disulfide link with 76, Redox-active
G149 (= G114) binding FAD
D348 (= D305) binding FAD
MLAH 354:357 (= MLAH 311:314) binding FAD

Sites not aligning to the query:

1:31 modified: transit peptide, Mitochondrion

Query Sequence

>WP_012992228.1 NCBI__GCF_000025605.1:WP_012992228.1
MEFDLVIVGAGSGGYEAGLYAHRRGMKVAFVELSPETVGGNCLNRGCIPSKYMRYGAYMI
ERFSHMSRYGILPKGFHLSMPHLKEGRDNVVRTIRENFKKFAQHLGIPIFYGKGFLKDPN
TVVVEGADITLRSRFVLLATGSSTVSVGGMVADGRYIHDTDTIWNLEEFPKRVVIVGGGA
VGVEFAYIFRMYGADVTLTELKDRLLPMAGIPEESSRFLARKLKKLGIDIKLKTTVNHYE
KTEEGLKVSFTDGTQLTTDIILLAVGRKPNTEGFKETGIEMDERGFVVVDKHCRTNLPSV
YACGDITSPLMLAHKAMYEGKVAVSHMLGDEGWEKNDTLMPKIIYSAYEVASVGLTEDEA
EDEGYHVRVGVVSFVTNPKAMDDGENEGFVRVVVEERTKRILGCHILGPNAGELLHQVIH
AMKAGLGADFMSRSVYSHPSLSEAIGQASMEVHFGPITWTKRS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory