SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013009628.1 NCBI__GCF_000025725.1:WP_013009628.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 4 hits to proteins with known functional sites (download)

6j2lA Crystal structure of bi-functional enzyme (see paper)
46% identity, 87% coverage: 3:99/111 of query aligns to 8:104/200 of 6j2lA

query
sites
6j2lA

K

E

I

L

D

D

W

W

K

E

K

K

Q

T

G

D

G

G

L

L

L

M

P

P

A

V

I

I

A

V

Q

Q

D

H

V

A

E

V

T

S

K

G

E

E

V

V

L

L

M

M

L

L

A

G

Y

Y

V

M

N

N

K

P

D

E

A

A

L

L

R

D

L

K

S

T

F

I

E

E

T

S

G

G

Y

K

A

V

H

T

Y

F

Y

F

S

S

R

R

S

T

R

K

D

Q

Q

R

L

L

W

W

K

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

L

F

Q

L

K

N

I

V

V

V

S

S

V

I

F

A

L

P

D
|
D

C

C

D
|
D

G

N

D
|
D

T

T

I

L

L

L

Y

V

L

L

V

A

N

N

Q

P

T

I

G

G

A

P

A

T

C
|
C

H

H

T

K

G

G

E

T

R

S

T

S

C
|
C

F

F

binding zinc ion: D79 (= D74), D81 (= D76), D83 (= D78), C96 (= C91), C103 (= C98)

Sites not aligning to the query:

binding zinc ion: 152, 171, 174

7bgnA Crystal structure of mthisn2-amp complex, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
43% identity, 95% coverage: 4:108/111 of query aligns to 9:112/204 of 7bgnA

query
sites
7bgnA

I

V

D

K

W

W

K

D

K

N

Q

K

G

G

G

-

L

L

L

A

P

V

A

A

I

I

A

A

Q

Q

D

N

V

V

E

D

T

T

K

G

E

A

V

I

L

L

M

M

L

Q

A

G

Y

F

V

A

N

N

K

R

D

E

A

A

L

V

R

A

L

T

S

T

F

I

E

S

T

S

G

R

Y

K

A

A

H

T

Y

F

Y

Y

S

S

R

R

S

S

R

R

D

S

Q

S

L

L

W
|
W

K

T

K
|
K

G
|
G

E

E

T
|
T

S
|
S

G

N

H

N

L

F

Q

I

K

N

I

V

V

H

S

D

V

V

F

F

L

L

D
|
D

C

C

D
|
D

G

R

D
|
D

T

S

I

I

L

I

Y

Y

L

L

V

G

N

K

Q

P

T

D

G

G

A

P

A
x
T

C
|
C

H
|
H

T

T

G

G

E

A

R

E

T

T

C
|
C

F

Y

F

Y

T

T

R

P

L

V

E

F

D

D

V

L

G

N

E

K

binding adenosine monophosphate: W60 (= W56), K62 (= K58), G63 (= G59), T65 (= T61), S66 (= S62), T94 (≠ A90), H96 (= H92)
binding zinc ion: D78 (= D74), D80 (= D76), D82 (= D78), C95 (= C91), C102 (= C98)

Sites not aligning to the query:

binding adenosine monophosphate: 121, 128, 135, 136, 137, 180, 203, 204
binding zinc ion: 154, 174, 177

7bgmA Crystal structure of mthisn2, a bifunctional enzyme from the histidine biosynthetic pathway (see paper)
44% identity, 93% coverage: 4:106/111 of query aligns to 11:112/213 of 7bgmA

query
sites
7bgmA

I

V

D

K

W

W

K

D

K

N

Q

K

G

G

G

-

L

L

L

A

P

V

A

A

I

I

A

A

Q

Q

D

N

V

V

E

D

T

T

K

G

E

A

V

I

L

L

M

M

L

Q

A

G

Y

F

V

A

N

N

K

R

D

E

A

A

L

V

R

A

L

T

S

T

F

I

E

S

T

S

G

R

Y

K

A

A

H

T

Y

F

Y

Y

S

S

R

R

S

S

R

R

D

S

Q

S

L

L

W

W

K

T

K

K

G

G

E

E

T

T

S

S

G

N

H

N

L

F

Q

I

K

N

I

V

V

H

S

D

V

V

F

F

L

L

D
|
D

C

C

D
|
D

G

R

D
|
D

T

S

I

I

L

I

Y

Y

L

L

V

G

N

K

Q

P

T

D

G

G

A

P

A

T

C
|
C

H

H

T

T

G

G

E

A

R

E

T

T

C
|
C

F

Y

F

Y

T

T

R

P

L

V

E

F

D

D

V

L

binding zinc ion: D80 (= D74), D82 (= D76), D84 (= D78), C97 (= C91), C104 (= C98)

Sites not aligning to the query:

binding zinc ion: 163, 183, 186

6j2lB Crystal structure of bi-functional enzyme (see paper)
41% identity, 94% coverage: 3:106/111 of query aligns to 7:113/185 of 6j2lB

query
sites
6j2lB

K

E

I

L

D

D

W

W

K

E

K

K

Q

T

G

D

G

G

L

L

L

M

P

P

A

V

I

I

A

V

Q

Q

D

H

V

A

E

V

T

S

K

G

E

E

V

V

L

L

M

M

L

L

A

G

Y

Y

V

M

N

N

K

P

D

E

A

A

L

L

R

D

L

K

S

T

F

I

E

E

T

S

G

G

Y

K

A

V

H

T

Y

F

Y

F

S

S

R

R

S

T

R

K

D

Q

Q

R

L

L

W

W

K

I

K

K

G

G

E

E

T

T

S

S

G

G

H

N

L

F

Q

L

K

N

I

V

V

V

S

S

V

I

F

A

L

P

D
|
D

C

C

D
|
D

G

N

D
|
D

T

T

I

L

L

L

Y

V

L

L

V

A

N

N

Q

P

T

I

G

G

A

P

A

S

C

S

-

C

-

F

-

G

H

N

T

T

G

A

E

H

R

Q

T

W

C

L

F

F

F

L

T

Y

R

Q

L

L

E

E

D

Q

V

L

binding zinc ion: D78 (= D74), D80 (= D76), D82 (= D78)

Sites not aligning to the query:

binding magnesium ion: 137, 140, 156, 159

Query Sequence

>WP_013009628.1 NCBI__GCF_000025725.1:WP_013009628.1
MIKIDWKKQGGLLPAIAQDVETKEVLMLAYVNKDALRLSFETGYAHYYSRSRDQLWKKGE
TSGHLQKIVSVFLDCDGDTILYLVNQTGAACHTGERTCFFTRLEDVGESDT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory