SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013009758.1 NCBI__GCF_000025725.1:WP_013009758.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P09114 Acetolactate synthase 2, chloroplastic; ALS II; Acetohydroxy-acid synthase II; Acetolactate synthase II; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 93:655/664 of P09114

query
sites
P09114

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P191 (≠ A102) mutation to A: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with L-568.
W568 (= W480) mutation to L: In S4-Hra; highly resistant to sulfonylurea herbicides; when associated with A-191.

P09342 Acetolactate synthase 1, chloroplastic; ALS I; Acetohydroxy-acid synthase I; Acetolactate synthase I; EC 2.2.1.6 from Nicotiana tabacum (Common tobacco) (see 2 papers)
44% identity, 99% coverage: 5:561/563 of query aligns to 96:658/667 of P09342

query
sites
P09342

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C161 (= C69) modified: Disulfide link with 307
P194 (≠ A102) mutation to Q: In C3; highly resistant to sulfonylurea herbicides.
C307 (≠ V213) modified: Disulfide link with 161

7tzzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase p197t mutant in complex with bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 7tzzA

query
sites
7tzzA

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binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: M266 (= M256), R292 (= R282), W489 (= W480), S568 (≠ A553)
binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), M263 (≠ W253), L264 (= L254), M266 (= M256), H267 (= H257), G286 (= G276), R288 (= R278), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414)
binding magnesium ion: A37 (≠ V28), T82 (= T72), S83 (= S73), Q122 (= Q112), Y381 (= Y372), D453 (= D444), M458 (= M449), Q461 (= Q452), N480 (= N471), H482 (≠ Y473), K533 (= K517)

5k2oA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, pyrithiobac (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/585 of 5k2oA

query
sites
5k2oA

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding 2-chloranyl-6-(4,6-dimethoxypyrimidin-2-yl)sulfanyl-benzoic acid: M266 (= M256), R292 (= R282), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), G286 (= G276), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), M428 (= M419), D453 (= D444), G454 (= G445), S455 (= S446), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)

8et4A Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with amidosulfuron (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 8et4A

query
sites
8et4A

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K

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-

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N

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binding 2-[3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-2-[(1~{S})-1-(dioxidanyl)-1-oxidanyl-ethyl]-4-methyl-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), R292 (= R282), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414)
binding magnesium ion: F370 (≠ Y361), D453 (= D444), M458 (= M449), Q461 (= Q452), N480 (= N471), H482 (≠ Y473), K533 (= K517)
binding N-{[(4,6-dimethoxypyrimidin-2-yl)carbamoyl]sulfamoyl}-N-methylmethanesulfonamide: M266 (= M256), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A553)

5wj1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a triazolopyrimidine herbicide, penoxsulam (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 5wj1A

query
sites
5wj1A

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H

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H

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M

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), M263 (≠ W253), L264 (= L254), G286 (= G276), R288 (= R278), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M266 (= M256), D291 (= D281), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A553)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), M428 (= M419), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)

5k6tA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, propoxycarbazone-sodium (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 5k6tA

query
sites
5k6tA

G

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I

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L

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x
V

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A

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Y

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A

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I

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x
H

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Q

M
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M

W

W

A

A

G

A

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Q

F

F

Y

Y

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N

Y

Y

K

K

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Q

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V

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G
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G

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M

G

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G

F

L

P

P

A

A

I

I

G

G

A

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L

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G

A

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N

P

P

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D

K

A

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I

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D

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G

D
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D

G
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S

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M

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M

N

N

I

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Q

Q

E

E

I

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C

A

T

T

A

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Y

E

N

N

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L

A

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V

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K

V

V

A

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I

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L

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N
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x
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L

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M
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I
x
V

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Q

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D

M

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F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

E

A

D

D

V

L

E

R

G

E

A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

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x
S

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G

A

G

A

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E

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M

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I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding methyl 2-[(4-methyl-5-oxidanylidene-3-propoxy-1,2,4-triazol-1-yl)carbonylsulfamoyl]benzoate: H267 (= H257), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), G286 (= G276), R288 (= R278), D290 (≠ S280), R292 (= R282), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), G454 (= G445), S455 (= S446), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475)

5k6rA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylamino-carbonyl-triazolinone herbicide, thiencarbazone-methyl (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 5k6rA

query
sites
5k6rA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

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V

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E

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F

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A

Y
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Y

P

P

G
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G

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x
A

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x
S

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G

E

I

I

Y

H

D

Q

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A

L

L

F

T

-

R

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S

S

D

S

I

I

K

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N

I

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L

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E
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E

Q

Q

G

G

I

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F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

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S

G

G

K

K

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G

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C

F

I

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A

T
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T

S

S

G

G

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P

G

G

A

A

T

T

N

N

L

L

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V

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S

G

G

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L

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A

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D

A

A

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L

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L

L

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E

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R

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K

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I

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G

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A

A

I

I

C

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E

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W

A

A

G

A

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F

F

Y

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Y

Y

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G
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G

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G

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F

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A

A

I

I

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A

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D

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D

D

I

I

A

D

G

G

D
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S

F

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M

I

M
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M

N

N

I

V

Q

Q

E

E

I

L

C

A

T

T

A

I

V

R

Q

V

Y

E

N

N

V

L

A

P

V

V

K

K

V

V

A

L

I

L

L

L

N

N

N
|
N

K

Q

Y
x
H

L
|
L

G
|
G

M
|
M

I
x
V

R

M

Q

Q

W
|
W

Q

E

N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

E

A

D

D

V

L

E

R

G

E

A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding methyl 4-[(3-methoxy-4-methyl-5-oxidanylidene-1,2,4-triazol-1-yl)carbonylsulfamoyl]-5-methyl-thiophene-3-carboxylate: R292 (= R282), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), M266 (= M256), G286 (= G276), R288 (= R278), R292 (= R282), V293 (≠ S283), D310 (= D300), I311 (≠ V301), G328 (= G318), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding 2-{3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-4-methyl-2-oxo-2,3-dihydro-1,3-thiazol-5-yl}ethyl trihydrogendiphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), D453 (= D444), G454 (= G445), S455 (= S446), M458 (= M449), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)

1z8nA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with an imidazolinone herbicide, imazaquin (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1z8nA

query
sites
1z8nA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y
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Y

P

P

G
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G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

V

V

A

P

S

R

N

R

L

M

I

I

G

G

G

T

D

D

A

A

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F

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E
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E

A

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I

I

I

V

G

E

I

V

T

T

R

R

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S

I

I

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T

K
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K

H

H

S

N

Y

Y

L

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I

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N

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N

D

P

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E

E

D

I

I

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1h-imidazol-2-yl)quinoline-3-carboxylic acid: K135 (= K125), R161 (= R151), Y191 (= Y181), R194 (vs. gap), D291 (= D281), R292 (= R282), D312 (= D302), W489 (= W480), G569 (= G554)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), R292 (= R282), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471)
binding thiamine diphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), G452 (= G443), G454 (= G445), S455 (= S446), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)

1yi1A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, tribenuron methyl (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1yi1A

query
sites
1yi1A

G

G

A

A

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding methyl 2-[4-methoxy-6-methyl-1,3,5-trazin-2-yl(methyl)carbamoylsulfamoyl]benzoate: D291 (= D281), R292 (= R282), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), M263 (≠ W253), L264 (= L254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)

1yi0A Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, sulfometuron methyl (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1yi0A

query
sites
1yi0A

G

G

A

A

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D

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P

G

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A

A

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N

N

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T

K

K

H

H

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N

Y

Y

L

L

I

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N

M

N

D

P

V

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E

E

D

I

I

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P

T

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V

I

I

I

R

E

E

E

A

A

F

F

Y

F

I

L

A

A

A

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S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

L

V

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K
|
K

D

D

I

I

M

Q

A

Q

Q

Q

K

L

A

A

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I

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W

W

P

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S

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K

A

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M

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L

L

P

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G

G

Y

Y

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M

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H

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L

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Q

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G

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A

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D

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M

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L

L

T

T

G

G

V

I

P

P

V

V

I

A

S

S

S
x
T

F
x
L

L
x
M

G

G

Q

L

G

G

G

S

I

Y

P

P

G

C

T

D

H

D

E

E

N

L

Y

S

I

L

G

H

W

M

L
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L

G
|
G

M
|
M

H
|
H

G

G

N

T

Y

V

A

Y

S

A

N

N

M

Y

A

A

M

V

A

E

E

H

P

S

D

D

F

L

I

L

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L

A

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V

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G

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x
V

R
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R

F

F

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x
D

D
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D

R
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R

S
x
V

T

T

G

G

R

K

L

L

D

E

G

A

F

F

A

A

P

S

N

R

A

A

K

K

V

I

A

V

H

H

I

I

D
|
D

V
x
I

D

D

P

S

S

A

S

E

I

I

S

G

K

K

N

N

V

K

R

T

I

P

D

H

V

V

P

S

I

V

V

C

G
|
G

D
|
D

C
x
V

K

K

V

L

V

-

L

-

N

-

Q

-

A

A

L

L

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G

I

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D

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K

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Y

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N

A

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K

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A

L

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K

K

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D

-

F

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W

W

M

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K

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V

G

G

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Q

N

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M
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M

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W

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A

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A

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F

Y

Y

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Y

Y

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K

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R

Q

Q

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W

V

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G
|
G

L

L

G
|
G

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M

G

G

Y

F

G

G

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P

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I

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G

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A

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D

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A

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V

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D

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F

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M

M

N

N

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Q

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N

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L

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K

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V

A

L

I

L

L

L

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N

N
|
N

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x
H

L
|
L

G

G

M
|
M

I
x
V

R

M

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|
W

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N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

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Q

-

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D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

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A

D

D

V

L

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R

G

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A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

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H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

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active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding methyl 2-[({[(4,6-dimethylpyrimidin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D281), R292 (= R282), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), R292 (= R282), V293 (≠ S283), D310 (= D300), I311 (≠ V301), G328 (= G318), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)

1yhzA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, chlorsulfuron (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1yhzA

query
sites
1yhzA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

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V

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P

L

L

V

V

V

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F

I

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T

G

G

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Q

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V

A

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N

R

L

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I

I

G

G

G

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D

D

A

A

F
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F

Q
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Q

E
|
E

A

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P

I

I

I

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G

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I

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R

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S

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K

K

H

H

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Y

Y

L

L

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N

M

N

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D

I

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T

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I

I

I

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E

E

A

A

F

F

Y

F

I

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A

A

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S

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G

G

R
|
R

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G

G

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L

I

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D

D

L

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K

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G

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L

L

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S

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T

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L

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M

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N

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S

I

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G

H

W

M

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L

G

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M

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H

G

G

N

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Y

V

A

Y

S

A

N

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M

Y

A

A

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E

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D

F

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I

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A

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x
V

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R

F

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x
D

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D

R
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R

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x
V

T

T

G

G

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K

L

L

D

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G

A

F

F

A

A

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N

R

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K

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I

A

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H

H

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D
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D

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I

D

D

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A

S

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I

I

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G

K

K

N

N

V

K

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H

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V

P

S

I

V

V

C

G

G

D
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D

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x
V

K

K

V

L

V

-

L

-

N

-

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A

A

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D

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W

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Y

Y

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A

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G

G

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A

A

I

I

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G

V
|
V

G

G

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N

H

Q
|
Q

M
|
M

W

W

A

A

G

A

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Q

F

F

Y

Y

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Y

Y

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W

V

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S

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|
G

L

L

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|
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T

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|
M

G

G

Y

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D

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|
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S

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F

M

I

M

M

N

N

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N

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L

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V

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L

I

L

L

L

N

N

N
|
N

K

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Y
x
H

L
|
L

G

G

M
|
M

I
x
V

R

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|
W

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N

D

M

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F

F

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N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

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D

D

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C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

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A

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A

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C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

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A

D

D

V

L

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R

G

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A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding 1-(2-chlorophenylsulfonyl)-3-(4-methoxy-6-methyl-l,3,5-triazin-2-yl)urea: D291 (= D281), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)

1yhyA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide, metsulfuron methyl (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1yhyA

query
sites
1yhyA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

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V

A

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S

R

N

R

L

M

I

I

G

G

G

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

I

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K

K

H

H

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N

Y

Y

L

L

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N

M

N

D

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E

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D

I

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V

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I

I

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E

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A

A

F

F

Y

F

I

L

A

A

A

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S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

L

V

P

P

K
|
K

D

D

I

I

M

Q

A

Q

Q

Q

K

L

A

A

E

I

F

P

K

N

W

W

P

E

S

Q

K

A

I

M

E

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

N

K

P

P

T

P

V

E

K

D

G

S

H

H

L

L

G

E

Q

Q

I

I

K

-

M

-

L

V

R

R

M

L

L

I

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S

G

E

A

S

K

K

P

P

V

V

I

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Y

Y

M

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G
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G

V

C

I

L

L

-

S

-

G

N

A

S

S

S

E

D

E

E

M

L

T

G

K

R

L

F

S

V

E

E

L

L

T

T

G

G

V

I

P

P

V

V

I

A

S

S

S
x
T

F
x
L

L
x
M

G

G

Q

L

G

G

G

S

I

Y

P

P

G

C

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D

H

D

E

E

N

L

Y

S

I

L

G

H

W

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

N

T

Y

V

A

Y

S

A

N

N

M

Y

A

A

M

V

A

E

E

H

P

S

D

D

F

L

I

L

L

L

A

A

V

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G

T
x
V

R
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R

F

F

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x
D

D
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D

R
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R

S
x
V

T

T

G

G

R

K

L

L

D

E

G

A

F

F

A

A

P

S

N

R

A

A

K

K

V

I

A

V

H

H

I

I

D
|
D

V
x
I

D

D

P

S

S

A

S

E

I

I

S

G

K

K

N

N

V

K

R

T

I

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

C
x
V

K

K

V

L

V

-

L

-

N

-

Q

-

A

A

L

L

S

Q

E

G

I

M

D

N

K

K

Y

V

S

L

W

E

D

N

K

R

N

A

L

E

K

E

A

L

R

K

K

L

D

D

-

F

-

G

-

V

W

W

M

R

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N

K

E

I

L

K

N

A

V

W

Q

D

K

K

Q

K

K

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P

P

F

L

G

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Y

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D

K

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F

S

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P

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Y

Y

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A

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K

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L

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D

K

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L

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K

D

G

G

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K

A

A

I

I

C

S

T

T

E

G

V
|
V

G

G

Q

Q

N

H

Q
|
Q

M
|
M

W

W

A

A

G

A

Q

Q

F

F

Y

Y

K

N

Y

Y

K

K

N

K

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P

R

R

Q

Q

F

W

V

L

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S

G
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G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

G

A

C

N

P

P

D

D

K

A

E

I

V

V

F

V

D

D

I

I

A

D

G

G

D
|
D

G

G

S

S

F

F

M

I

M

M

N

N

I

V

Q

Q

E

E

I

L

C

A

T

T

A

I

V

R

Q

V

Y

E

N

N

V

L

A

P

V

V

K

K

V

V

A

L

I

L

L

L

N

N

N
|
N

K

Q

Y
x
H

L
|
L

G

G

M
|
M

I
x
V

R

M

Q

Q

W
|
W

Q

E

N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

E

A

D

D

V

L

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R

G

E

A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding methyl 2-[({[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl}amino)sulfonyl]benzoate: D291 (= D281), R292 (= R282), V486 (≠ I477), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), G265 (= G255), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)

1ybhA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a sulfonylurea herbicide chlorimuron ethyl (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/582 of 1ybhA

query
sites
1ybhA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

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V

D

E

L

T

I

V

F

F

G

A

Y
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Y

P

P

G
|
G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

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S

G

G

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K

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P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

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V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

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A

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N

R

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I

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T

D

D

A

A

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F

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Q

E
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E

A

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D

P

I

I

I

V

G

E

I

V

T

T

R

R

P

S

I

I

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T

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K

H

H

S

N

Y

Y

L

L

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N

M

N

D

P

V

E

E

E

D

I

I

T

P

T

R

V

I

I

I

R

E

E

E

A

A

F

F

Y

F

I

L

A

A

A

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S

S

G

G

R
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R

P

P

G

G

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P

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V

V

L

I

V

D

D

L

V

P

P

K
|
K

D

D

I

I

M

Q

A

Q

Q

Q

K

L

A

A

E

I

F

P

K

N

W

W

P

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S

Q

K

A

I

M

E

R

L

L

P

P

G

G

Y

Y

-

M

-

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-

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M

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N

K

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Q

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M

-

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A

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K

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P

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Y

M

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G

G
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G

V

C

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L

L

-

S

-

G

N

A

S

S

S

E

D

E

E

M

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E

L

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x
T

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x
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M

G

G

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G

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W

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L

G

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M

H
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H

G

G

N

T

Y

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Y

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A

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Y

A

A

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A

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x
V

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R

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x
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R

S
x
V

T

T

G

G

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L

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E

G

A

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F

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A

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R

A

A

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K

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H

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D

V
x
I

D

D

P

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S

A

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I

I

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G

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K

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N

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C

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D

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x
V

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K

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-

L

-

N

-

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-

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A

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L

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G

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Y

V

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N

A

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A

L

R

K

K

L

D

D

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G

-

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W

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N

A

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D

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A

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Y

D

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L

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D

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K

A

A

I

I

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T

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E

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V

G

G

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N

H

Q
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Q

M
|
M

W

W

A

A

G

A

Q

Q

F

F

Y

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Y

Y

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K

N

K

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R

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F

W

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S

G
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G

L

L

G
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T

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M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

L

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A

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N

P

P

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D

K

A

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D

D

I

I

A

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D

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F

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M

M

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N

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E

E

I

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C

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T

T

A

I

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V

Y

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N

V

L

A

P

V

V

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K

V

V

A

L

I

L

L

L

N

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N
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N

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x
H

L
|
L

G

G

M
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M

I
x
V

R

M

Q

Q

W
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W

Q

E

N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

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K

E

A

D

D

V

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G

E

A

A

I

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E

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M

L

L

K

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I

T

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P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl]benzoic acid ethyl ester: M266 (= M256), D291 (= D281), R292 (= R282), M485 (= M476), W489 (= W480), S568 (≠ A553)
binding flavin-adenine dinucleotide: R161 (= R151), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), M266 (= M256), H267 (= H257), G286 (= G276), V287 (≠ T277), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), Q404 (= Q395), M405 (= M396), G423 (= G414), G424 (= G415)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)

P17597 Acetolactate synthase, chloroplastic; AtALS; Acetohydroxy-acid synthase; Protein CHLORSULFURON RESISTANT 1; EC 2.2.1.6 from Arabidopsis thaliana (Mouse-ear cress) (see 8 papers)
44% identity, 99% coverage: 5:561/563 of query aligns to 99:661/670 of P17597

query
sites
P17597

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y

Y

P

P

G

G

V
x
A

L

S

L
x
M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T

T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S
|
S

V

V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

V

V

A
x
P

S

R

N
x
R

L

M

I

I

G

G

G

T

D

D

A

A

F

F

Q
|
Q

E

E

A

T

D

P

I

I

I

V

G

E

I

V

T

T

R

R

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I

I

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T

K
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K

H

H

S

N

Y

Y

L

L

I

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N

M

N

D

P

V

E

E

E

D

I

I

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P

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V

I

I

I

R

E

E

E

A

A

F

F

Y

F

I

L

A

A

A

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S

S

G

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R
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R

P

P

G

G

P

P

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V

V

L

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D

L

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K

D

D

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A

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A

A

E

I

F

P

K

N

W

W

P

E

S

Q

K

A

I

M

E

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

N

K

P

P

T

P

V

E

K

D

G

S

H

H

L

L

G

E

Q

Q

I

I

K

-

M

-

L

V

R

R

M

L

L

I

E

S

G

E

A

S

K

K

P

P

V

V

I

L

Y

Y

M

V

G

G

G
|
G

G

G

V

C

I

L

L

-

S

-

G

N

A

S

S

S

E

D

E

E

M

L

T

G

K

R

L

F

S

V

E

E

L

L

T

T

G

G

V

I

P

P

V

V

I

A

S

S

S
x
T

F
x
L

L

M

G

G

Q

L

G

G

G

S

I

Y

P

P

G
x
C

T

D

H

D

E

E

N

L

Y

S

I

L

G

H

W

M

L
|
L

G
|
G

M
|
M

H
|
H

G

G

N

T

Y

V

A

Y

S

A

N

N

M

Y

A

A

M

V

A

E

E

H

P

S

D

D

F

L

I

L

L

L

A

A

V

F

G
|
G

T
x
V

R
|
R

F
|
F

S
x
D

D
|
D

R
|
R

S

V

T

T

G

G

R

K

L

L

D

E

G

A

F

F

A

A

P

S

N

R

A

A

K

K

V

I

A

V

H

H

I

I

D
|
D

V
x
I

D

D

P

S

S

A

S

E

I

I

S

G

K

K

N

N

V

K

R

T

I

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

C
x
V

K

K

V

L

V

-

L

-

N

-

Q

-

A

A

L

L

S

Q

E

G

I

M

D

N

K

K

Y

V

S

L

W

E

D

N

K

R

N

A

L

E

K

E

A

L

R

K

K

L

D

D

-

F

-

G

-

V

W

W

M

R

E

N

K

E

I

L

K

N

A

V

W

Q

D

K

K

Q

K

K

Q

F

P

P

F

L

G

S

Y

F

D

K

R

T

E

F

S

G

T

E

L

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

V

I

E

K

S

V

I

L

Y

D

K

E

V

L

T

T

K

D

G

G

E

K

A

A

I

I

C

S

T

T

E

G

V

V

G

G

Q
|
Q

N
x
H

Q

Q

M

M

W

W

A

A

G

A

Q

Q

F

F

Y

Y

K

N

Y

Y

K

K

N

K

P

P

R

R

Q

Q

F

W

V

L

S

S

G
|
G

L

L

G
|
G

T
x
A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

G

A

C

N

P

P

D

D

K

A

E

I

V

V

F

V

D

D

I

I

A

D

G

G

D
|
D

G
|
G

S
|
S

F

F

M

I

M

M

N

N

I

V

Q

Q

E

E

I

L

C

A

T

T

A

I

V

R

Q

V

Y

E

N

N

V

L

A

P

V

V

K

K

V

V

A

L

I

L

L

L

N

N

N
|
N

K
x
Q

Y
x
H

L
|
L

G
|
G

M
|
M

I

V

R

M

Q

Q

W
|
W

Q

E

N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

E

A

D

D

V

L

E

R

G

E

A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

G

K

-

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R

H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A
x
S

G

G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

A122 (≠ V28) mutation to V: Reduced catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
M124 (≠ L30) mutation to E: Reduced catalytic activity. Resistant to imidazolinone herbicides and reduced sensitivity to sulfonylurea herbicides.; mutation to I: No effect on catalytic activity. Increased resistance to imidazolinone herbicides.
E144 (= E49) binding thiamine diphosphate
S186 (= S91) binding FAD
P197 (≠ A102) mutation to S: In csr1-1/GH50; resistant to sulfonylurea but not to imidazolinone herbicides.
R199 (≠ N104) mutation R->A,E: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.
Q207 (= Q112) binding thiamine diphosphate
K220 (= K125) binding (R)-imazaquin
R246 (= R151) binding (R)-imazaquin; binding FAD
K256 (= K161) binding chlorimuron-ethyl
G308 (= G211) binding FAD
TL 331:332 (≠ SF 236:237) binding FAD
C340 (≠ G245) modified: Cysteine sulfinic acid (-SO2H)
LGMH 349:352 (= LGMH 254:257) binding FAD
GVRFD 371:375 (≠ GTRFS 276:280) binding FAD
DR 376:377 (= DR 281:282) binding chlorimuron-ethyl
DI 395:396 (≠ DV 300:301) binding FAD
DV 414:415 (≠ DC 319:320) binding FAD
QH 487:488 (≠ QN 393:394) binding thiamine diphosphate
GG 508:509 (= GG 414:415) binding FAD
GAM 511:513 (≠ GTM 417:419) binding thiamine diphosphate
D538 (= D444) binding Mg(2+)
DGS 538:540 (= DGS 444:446) binding thiamine diphosphate
N565 (= N471) binding Mg(2+)
NQHLGM 565:570 (≠ NKYLGM 471:476) binding thiamine diphosphate
H567 (≠ Y473) binding Mg(2+)
W574 (= W480) binding chlorimuron-ethyl; mutation to L: Increased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.; mutation to S: Slightly decreased catalytic activity. Resistant to imidazolinone and sulfonylurea herbicides.
S653 (≠ A553) binding chlorimuron-ethyl; mutation to A: No effect on catalytic activity or sensitivity to herbicides.; mutation to F: No effect on catalytic activity. Resistant to imidazolinone herbicides and also slightly sulfonylurea-resistant.; mutation to N: In csr1-2/GH90; no effect on catalytic activity. Resistant to imidazolinone but not to sulfonylurea herbicides.; mutation to T: No effect on catalytic activity. Resistant to imidazolinone herbicides but not to sulfonylurea herbicides.

5k3sA Crystal structure of arabidopsis thaliana acetohydroxyacid synthase in complex with a pyrimidinyl-benzoate herbicide, bispyribac-sodium (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 14:576/583 of 5k3sA

query
sites
5k3sA

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

V

V

A

P

S

R

N

R

L

M

I

I

G

G

G

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

I

V

G

E

I

V

T

T

R

R

P

S

I

I

V

T

K

K

H

H

S

N

Y

Y

L

L

I

V

N

M

N

D

P

V

E

E

E

D

I

I

T

P

T

R

V

I

I

I

R

E

E

E

A

A

F

F

Y

F

I

L

A

A

A

T

S

S

G

G

R
|
R

P

P

G

G

P

P

V

V

V

L

I

V

D

D

L

V

P

P

K
|
K

D

D

I

I

M

Q

A

Q

Q

Q

K

L

A

A

E

I

F

P

K

N

W

W

P

E

S

Q

K

A

I

M

E

R

L

L

P

P

G

G

Y

Y

-

M

-

S

-

R

-

M

-

P

N

K

P

P

T

P

V

E

K

D

G

S

H

H

L

L

G

E

Q

Q

I

I

K

-

M

-

L

V

R

R

M

L

L

I

E

S

G

E

A

S

K

K

P

P

V

V

I

L

Y

Y

M

V

G
|
G

V

C

I

L

L

-

S

-

G

N

A

S

S

S

E

D

E

E

M

L

T

G

K

R

L

F

S

V

E

E

L

L

T

T

G

G

V

I

P

P

V

V

I

A

S

S

S
x
T

F
x
L

L
x
M

G

G

Q

L

G

G

G

S

I

Y

P

P

G

C

T

D

H

D

E

E

N

L

Y

S

I

L

G

H

W

M

L
|
L

G

G

M
|
M

H

H

G

G

N

T

Y

V

A

Y

S

A

N

N

M

Y

A

A

M

V

A

E

E

H

P

S

D

D

F

L

I

L

L

L

A

A

V

F

G
|
G

T

V

R
|
R

F

F

S
x
D

D

D

R
|
R

S
x
V

T

T

G

G

R

K

L

L

D

E

G

A

F

F

A

A

P

S

N

R

A

A

K

K

V

I

A

V

H

H

I

I

D
|
D

V
x
I

D

D

P

S

S

A

S

E

I

I

S

G

K

K

N

N

V

K

R

T

I

P

D

H

V

V

P

S

I

V

V

C

G

G

D
|
D

C
x
V

K

K

V

L

V

-

L

-

N

-

Q

-

A

A

L

L

S

Q

E

G

I

M

D

N

K

K

Y

V

S

L

W

E

D

N

K

R

N

A

L

E

K

E

A

L

R

K

K

L

D

D

-

F

-

G

-

V

W

W

M

R

E

N

K

E

I

L

K

N

A

V

W

Q

D

K

K

Q

K

K

Q

F

P

P

F

L

G

S

Y

F

D

K

R

T

E

F

S

G

T

E

L

A

I

I

K

P

P

P

Q

Q

Y

Y

V

A

V

I

E

K

S

V

I

L

Y

D

K

E

V

L

T

T

K

D

G

G

E

K

A

A

I

I

C

S

T

T

E

G

V
|
V

G
|
G

Q
|
Q

N
x
H

Q

Q

M
|
M

W

W

A

A

G

A

Q

Q

F

F

Y

Y

K

N

Y

Y

K

K

N

K

P

P

R

R

Q

Q

F

W

V

L

S

S

G
|
G

G

G

L

L

G
|
G

T

A

M
|
M

G

G

Y

F

G

G

F

L

P

P

A

A

I

I

G

G

A

A

K

S

L

V

G

A

C

N

P

P

D

D

K

A

E

I

V

V

F

V

D

D

I

I

A

D

G

G

D
|
D

G
|
G

S
|
S

F

F

M

I

M

M

N

N

I

V

Q

Q

E

E

I

L

C

A

T

T

A

I

V

R

Q

V

Y

E

N

N

V

L

A

P

V

V

K

K

V

V

A

L

I

L

L

L

N

N

N
|
N

K

Q

Y
x
H

L
|
L

G
|
G

M
|
M

I
x
V

R

M

Q

Q

W
|
W

Q

E

N

D

M

R

F

F

F

Y

N

K

N

A

R

N

Y

R

S

A

Q

H

C

T

C

F

L

L

-

G

-

D

-

P

-

A

-

Q

-

E

-

D

D

E

C

I

Q

F

P

P

D

N

F

M

V

L

K

L

L

F

A

A

E

A

S

A

F

C

N

G

A

I

V

P

G

A

M

A

R

R

I

V

D

T

R

K

K

K

E

A

D

D

V

L

E

R

G

E

A

A

I

I

R

Q

E

T

S

M

L

L

K

D

I

T

K

P

D

-

K

G

P

P

V

Y

I

L

M

L

D

D

F

V

T

I

V

C

D

P

R
x
H

E

Q

E

E

N

H

V

V

F

L

P

P

M

M

I

I

P

P

A

S

G
|
G

A

G

A

T

I

F

N

N

E

D

M

V

L

I

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), S38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), M266 (= M256), V293 (≠ S283), V400 (= V391), G426 (= G417), M428 (= M419), D453 (= D444), N480 (= N471), H482 (≠ Y473), L483 (= L474), M485 (= M476), V486 (≠ I477), W489 (= W480), H558 (≠ R543)
binding 2,6-bis[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid: R292 (= R282), M485 (= M476), W489 (= W480), G569 (= G554)
binding flavin-adenine dinucleotide: R161 (= R151), G222 (= G210), G223 (= G211), G224 (= G212), T246 (≠ S236), L247 (≠ F237), M248 (≠ L238), L264 (= L254), M266 (= M256), G286 (= G276), R288 (= R278), D290 (≠ S280), V293 (≠ S283), D310 (= D300), I311 (≠ V301), D329 (= D319), V330 (≠ C320), M405 (= M396), G423 (= G414)
binding magnesium ion: D453 (= D444), N480 (= N471), H482 (≠ Y473)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V400 (= V391), G401 (= G392), Q402 (= Q393), H403 (≠ N394), G426 (= G417), M428 (= M419), D453 (= D444), G454 (= G445), S455 (= S446), N480 (= N471), H482 (≠ Y473), L483 (= L474), G484 (= G475), M485 (= M476), V486 (≠ I477)

3ea4A Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron-ester (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 13:575/582 of 3ea4A

query
sites
3ea4A

G

G

A

A

E

D

I

I

L

L

V

V

E

E

S

A

L

L

R

E

K

R

Q

Q

G

G

V

V

D

E

L

T

I

V

F

F

G

A

Y
|
Y

P

P

G
|
G

V
x
A

L
x
S

L

M

G

E

I

I

Y

H

D

Q

T

A

L

L

F

T

-

R

D

S

S

S

D

S

I

I

K

R

H

N

I

V

L

L

P

P

R

R

H

H

E
|
E

Q

Q

G

G

I

V

H

F

A

A

D

E

G

G

Y

Y

A

A

R

R

A

S

T

S

G

G

K

K

V

P

G

G

V

I

C

C

F

I

G

A

T
|
T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

L

V

V

T

S

G

G

I

L

C

A

N

D

A

A

H

L

M

L

D

D

S

S

V

V

P

P

L

L

V

V

V

A

F

I

T

T

G

G

Q

Q

V

V

A

P

S

R

N

R

L

M

I

I

G

G

G

T

D

D

A

A

F
|
F

Q
|
Q

E
|
E

A

T

D

P

I

I

I

V

G

E

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T

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R

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I

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R

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x
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M

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A

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Y

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G

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G

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N

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Q

E

E

I

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N

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A

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-

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-

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-

P

-

A

-

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-

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-

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D

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C

I

Q

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P

P

D

N

F

M

V

L

K

L

L

F

A

A

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A

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A

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C

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A

I

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P

G

A

M

A

R

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I

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T

R

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K

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A

D

D

V

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R

G

E

A

A

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Q

E

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M

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L

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D

I

T

K

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D

-

K

G

P

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M

L

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D

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H

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H

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L

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P

M

M

I

I

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x
S

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active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (≠ V28), S37 (≠ L29), E58 (= E49), T81 (= T72), F120 (= F111), Q121 (= Q112), E122 (= E113), K170 (= K161), M265 (= M256), V292 (≠ S283), V399 (= V391), G425 (= G417), M427 (= M419), D452 (= D444), N479 (= N471), H481 (≠ Y473), L482 (= L474), M484 (= M476), V485 (≠ I477), W488 (= W480), H557 (≠ R543)
binding methyl 2-{[(4-methylpyrimidin-2-yl)carbamoyl]sulfamoyl}benzoate: D290 (= D281), R291 (= R282), W488 (= W480), S567 (≠ A553)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R151), G221 (= G210), G222 (= G211), G223 (= G212), T245 (≠ S236), L246 (≠ F237), M247 (≠ L238), L263 (= L254), G264 (= G255), M265 (= M256), H266 (= H257), G285 (= G276), R287 (= R278), D289 (≠ S280), R291 (= R282), D309 (= D300), I310 (≠ V301), G327 (= G318), D328 (= D319), V329 (≠ C320), M404 (= M396), G422 (= G414)
binding magnesium ion: D452 (= D444), N479 (= N471), H481 (≠ Y473)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V391), G400 (= G392), Q401 (= Q393), H402 (≠ N394), M427 (= M419), G451 (= G443), D452 (= D444), G453 (= G445), S454 (= S446), N479 (= N471), H481 (≠ Y473), L482 (= L474), G483 (= G475), M484 (= M476), V485 (≠ I477)

3e9yA Arabidopsis thaliana acetohydroxyacid synthase in complex with monosulfuron (see paper)
44% identity, 99% coverage: 5:561/563 of query aligns to 13:575/582 of 3e9yA

query
sites
3e9yA

G

G

A

A

E

D

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I

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A

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T

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N

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-

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-

A

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P

D

N

F

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A

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H

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H

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M

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S

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G

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G

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active site: Y32 (= Y24), G34 (= G26), G35 (= G27), A36 (≠ V28), S37 (≠ L29), E58 (= E49), T81 (= T72), F120 (= F111), Q121 (= Q112), E122 (= E113), K170 (= K161), M265 (= M256), V292 (≠ S283), V399 (= V391), G425 (= G417), M427 (= M419), D452 (= D444), N479 (= N471), H481 (≠ Y473), L482 (= L474), M484 (= M476), V485 (≠ I477), W488 (= W480), H557 (≠ R543)
binding N-[(4-methylpyrimidin-2-yl)carbamoyl]-2-nitrobenzenesulfonamide: D290 (= D281), R291 (= R282), W488 (= W480), S567 (≠ A553)
binding flavin-adenine dinucleotide-n5-isobutyl ketone: R160 (= R151), G221 (= G210), G222 (= G211), G223 (= G212), T245 (≠ S236), L246 (≠ F237), M247 (≠ L238), L263 (= L254), G285 (= G276), R287 (= R278), D289 (≠ S280), R291 (= R282), D309 (= D300), I310 (≠ V301), G327 (= G318), D328 (= D319), V329 (≠ C320), M404 (= M396), G422 (= G414)
binding magnesium ion: D452 (= D444), N479 (= N471), H481 (≠ Y473)
binding 2-[(2e)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-(1-hydroxyethylidene)-4-methyl-2,3-dihydro-1,3-thiazol-5-yl]ethyltrihydrogen diphosphate: V399 (= V391), G400 (= G392), Q401 (= Q393), H402 (≠ N394), M427 (= M419), G451 (= G443), G453 (= G445), S454 (= S446), N479 (= N471), H481 (≠ Y473), L482 (= L474), G483 (= G475), M484 (= M476), V485 (≠ I477)

6deqA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide penoxsulam (see paper)
42% identity, 99% coverage: 3:562/563 of query aligns to 14:580/601 of 6deqA

query
sites
6deqA

M

L

T

T

G

G

A

G

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E

I

I

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F

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H

E

E

S

M

L

M

R

L

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R

Q

H

G

K

V

V

D

D

L

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F

G

G

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A

G
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G

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x
A

L
x
I

L

L

G

P

I

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Y

F

D

D

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A

L

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F

Y

D

N

S

S

D

D

-

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I

F

K

K

H

F

I

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L

L

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R

R

H

H

E
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E

Q

Q

G

G

G

A

I

G

H

H

A

M

A

A

D

E

G

G

Y

Y

A

A

R

R

A

A

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S

G

G

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K

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P

G

G

V

V

C

V

F

L

G

V

T
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T

S

S

G

G

P

P

G

G

A

A

T

T

N

N

L

V

V

I

T

T

G

P

I

M

C

A

N

D

A

A

H

L

M

M

D

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L

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D

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L

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N

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x
V

V

K

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G

M

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R

I

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Q

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E

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I

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active site: Y35 (= Y24), G37 (= G26), G38 (= G27), A39 (≠ V28), I40 (≠ L29), E61 (= E49), T84 (= T72), F123 (= F111), Q124 (= Q112), E125 (= E113), K173 (= K161), K232 (≠ E221), M268 (= M256), V295 (≠ S283), V411 (= V391), L436 (= L416), G437 (= G417), M439 (= M419), D464 (= D444), N491 (= N471), E493 (≠ Y473), Q494 (≠ L474), M496 (= M476), V497 (≠ I477), W500 (= W480), L522 (= L503), N527 (= N508), V528 (≠ A509)
binding flavin-adenine dinucleotide: R163 (= R151), G221 (= G210), A222 (≠ G211), G223 (= G212), N226 (≠ L215), T248 (≠ S236), L249 (≠ F237), Q250 (≠ L238), L266 (= L254), G288 (= G276), A289 (≠ T277), R290 (= R278), D292 (≠ S280), R294 (= R282), V295 (≠ S283), E321 (≠ D300), I322 (≠ V301), D340 (= D319), V341 (≠ C320), M416 (= M396), G434 (= G414)
binding magnesium ion: D464 (= D444), N491 (= N471), E493 (≠ Y473)
binding 2-(2,2-difluoroethoxy)-N-(5,8-dimethoxy[1,2,4]triazolo[1,5-c]pyrimidin-2-yl)-6-(trifluoromethyl)benzenesulfonamide: M268 (= M256), R294 (= R282), M496 (= M476), V497 (≠ I477), W500 (= W480), A571 (= A553)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V411 (= V391), G412 (= G392), Q413 (= Q393), H414 (≠ N394), M439 (= M419), G463 (= G443), D464 (= D444), A465 (≠ G445), S466 (= S446), N491 (= N471), E493 (≠ Y473), Q494 (≠ L474), G495 (= G475), M496 (= M476), V497 (≠ I477)

6demA Crystal structure of candida albicans acetohydroxyacid synthase in complex with the herbicide bensulfuron methyl (see paper)
42% identity, 99% coverage: 3:562/563 of query aligns to 12:576/597 of 6demA

query
sites
6demA

M

L

T

T

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A

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E

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M
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T

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Q

T

E

E

I

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A

A

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A

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G

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A

A

P

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K

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C

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L

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x
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x
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M

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N

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R

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C

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L

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x
V

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G

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L

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K

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V

I

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M

L

D

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E

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K

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K

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P

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M

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L

active site: Y33 (= Y24), G35 (= G26), G36 (= G27), A37 (≠ V28), I38 (≠ L29), E59 (= E49), T82 (= T72), F121 (= F111), Q122 (= Q112), E123 (= E113), K171 (= K161), K228 (≠ E221), M264 (= M256), V291 (≠ S283), V407 (= V391), L432 (= L416), G433 (= G417), M435 (= M419), D460 (= D444), N487 (= N471), E489 (≠ Y473), Q490 (≠ L474), M492 (= M476), V493 (≠ I477), W496 (= W480), L518 (= L503), N523 (= N508), V524 (≠ A509)
binding methyl 2-[(4,6-dimethoxypyrimidin-2-yl)carbamoylsulfamoylmethyl]benzoate: M264 (= M256), D289 (= D281), R290 (= R282), M492 (= M476), W496 (= W480), A567 (= A553)
binding flavin-adenine dinucleotide: R161 (= R151), G217 (= G210), A218 (≠ G211), G219 (= G212), N222 (≠ L215), T244 (≠ S236), L245 (≠ F237), Q246 (≠ L238), L262 (= L254), G284 (= G276), A285 (≠ T277), R286 (= R278), D288 (≠ S280), R290 (= R282), V291 (≠ S283), E317 (≠ D300), I318 (≠ V301), N322 (≠ S305), D336 (= D319), V337 (≠ C320), M412 (= M396), G430 (= G414)
binding magnesium ion: D460 (= D444), N487 (= N471), E489 (≠ Y473)
binding (3z)-4-{[(4-amino-2-methylpyrimidin-5-yl)methyl]amino}-3-mercaptopent-3-en-1-yl trihydrogen diphosphate: V407 (= V391), G408 (= G392), Q409 (= Q393), H410 (≠ N394), M435 (= M419), G459 (= G443), D460 (= D444), A461 (≠ G445), S462 (= S446), M465 (= M449), N487 (= N471), E489 (≠ Y473), Q490 (≠ L474), G491 (= G475), M492 (= M476), V493 (≠ I477)

Query Sequence

>WP_013009758.1 NCBI__GCF_000025725.1:WP_013009758.1
MKMTGAEILVESLRKQGVDLIFGYPGGVLLGIYDTLFDSDIKHILPRHEQGGIHAADGYA
RATGKVGVCFGTSGPGATNLVTGICNAHMDSVPLVVFTGQVASNLIGGDAFQEADIIGIT
RPIVKHSYLINNPEEITTVIREAFYIAASGRPGPVVIDLPKDIMAQKAEFKWPSKIELPG
YNPTVKGHLGQIKKMLRMLEGAKKPVIYMGGGVILSGASEEMTKLSELTGVPVISSFLGQ
GGIPGTHENYIGWLGMHGNYASNMAMAEPDFILAVGTRFSDRSTGRLDGFAPNAKVAHID
VDPSSISKNVRIDVPIVGDCKVVLNQALSEIDKYSWDKNLKARKDWMEKIKAWDKKQPFG
YDRESTLIKPQYVVESIYKVTKGEAIICTEVGQNQMWAGQFYKYKNPRQFVSSGGLGTMG
YGFPAAIGAKLGCPDKEVFDIAGDGSFMMNIQEICTAVQYNVAVKVAILNNKYLGMIRQW
QNMFFNNRYSQCCLDCQPDFVKLAESFNAVGMRIDRKEDVEGAIRESLKIKDKPVIMDFT
VDREENVFPMIPAGAAINEMLLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory