SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013010412.1 NCBI__GCF_000025725.1:WP_013010412.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
34% identity, 97% coverage: 7:243/244 of query aligns to 11:249/249 of P12282

query
sites
P12282

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R14 (= R10) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ G40) mutation to A: No effect.
R73 (= R69) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ A124) mutation to A: No effect.; mutation to Y: No activity.
D130 (≠ P126) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C138) mutation to A: No effect.
C172 (= C167) mutation to A: No zinc bound and no enzyme activity.
C175 (= C170) mutation to A: No zinc bound and no enzyme activity.
C187 (≠ W179) mutation to A: No effect.
C231 (≠ M225) mutation to A: No effect.
C244 (= C238) mutation to A: No zinc bound and almost no enzyme activity.
C247 (= C241) mutation to A: No zinc bound and almost no enzyme activity.

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
36% identity, 99% coverage: 2:242/244 of query aligns to 66:310/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
34% identity, 97% coverage: 7:242/244 of query aligns to 10:240/240 of 1jwbB

query
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1jwbB

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active site: R13 (= R10), D129 (≠ P126)
binding adenosine monophosphate: G37 (= G34), G39 (= G36), G40 (= G37), D61 (≠ H58), F62 (≠ Y59), K85 (≠ R82), L108 (≠ R105), C127 (≠ A124), T128 (≠ R125), D129 (≠ P126), N130 (= N127), V133 (≠ E130)
binding zinc ion: C171 (= C167), C236 (= C238), C239 (= C241)

1jw9B Structure of the native moeb-moad protein complex (see paper)
34% identity, 97% coverage: 7:242/244 of query aligns to 10:240/240 of 1jw9B

query
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1jw9B

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active site: R13 (= R10), D129 (≠ P126)
binding zinc ion: C171 (= C167), C236 (= C238), C239 (= C241)

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
36% identity, 97% coverage: 6:242/244 of query aligns to 9:253/271 of Q72J02

query
sites
Q72J02

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L

A

M

A

A

V

A

E

E

A

A

V

L

K

K

I

V

I

L

T

L

K

G

H

I

G

G

T

K

P

P

L

L

K

A

G

G

K

H

M

L

H

L

I

Y

D

D

F

A

-

L

-

E

N

A

T

S

M

F

R

R

T

K

I

L

T

T

P

-

K

-

L

V

K

R

R

R

N

N

P

P

D

R

C

C

P

P

V

V

C

C

G

G

C192 (≠ W179) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
33% identity, 98% coverage: 6:243/244 of query aligns to 11:250/270 of D4GSF3

query
sites
D4GSF3

E

D

R

R

Y

Y

K

S

R

R

Q

H

I

I

M

I

L

M

K

D

D

E

F

V

G

G

R

P

E

E

K

G

Q

Q

D

G

I

R

L

L

K

L

N

S

S

S

S

R

V

V

F

V

I

V

G

V

G

G

I

A

G

G

L

L

G

G

G

A

A

P

T

A

A

I

A

Q

Y

Y

L

L

A

A

S

A

A

V

G

G

I

V

G

G

R

E

M

L

V

V

I

V

S

V

H

D

Y

D

G

D

N

V

L

V

T

E

E

R

S

S

N

N

M

L

N

Q

R

R

Q

Q

H

V

L

V

M

H

D

C

V

D

N

D

R

D

I

V

G

G

E

T

P

P

R

K

I

A

D

E

I

S

A

A

V

A

E

A

N

F

I

V

L

R

K

G

I

L

N

N

P

P

D

D

I

V

E

S

L

V

E

E

M

P

H

V

N

E

I

A

R

R

L

V

N

D

E

K

N

S

K

N

A

V

E

H

K

E

F

V

I

V

E

A

G

G

C

S

D

D

V

V

A

V

V

V

S

D

A

A

R

S

P

D

N

N

F

F

P

P

E

T

R

R

M

Y

A

L

M

L

N

N

N

D

A

V

C

C

V

R

K

F

L

E

G

G

V

I

P

P

M

L

I

V

E

H

A

G

A

A

M

I

D

Y

D

K

M

F

V

E

G

G

Y

Q

Y

A

F

T

N

T

I

L

F

V

P

P

H

-

E

D

T

G

A

P

C

C

L

Y

R

R

C

C

F

L

V

F

S

P

E

E

-

A

-

P

-

E

-

P

-

G

D

T

V

V

P

P

D

D

W
x
C

K

A

E

T

L

T

G

-

F

-

G

G

V

V

L

L

G

G

A

V

V

L

S

P

G

G

T

T

I

V

G

G

S

C

L

I

A

Q

A

A

V

T

E

E

A

A

V

M

K

K

I

L

T

L

K

D

H

E

G

G

T

E

P

A

L

L

K

D

G

G

K

R

M

L

H

F

I

Y

D

D

F

A

N

M

T

D

M

M

R

T

T

F

I

E

T

T

P

V

K

P

L

Y

K

R

R

T

N

N

P

P

D

D

C

C

P

P

V

V

C

C

G

G

R

E

C188 (≠ W179) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

1zud3 Structure of this-thif protein complex (see paper)
34% identity, 98% coverage: 5:242/244 of query aligns to 6:239/240 of 1zud3

query
sites
1zud3

Y

F

E

M

R

R

Y

Y

K

S

R
|
R

Q

Q

I

I

M
x
L

L

L

K
x
D

D

D

F

I

G

A

R

L

E

D

K

G

Q

Q

D

Q

I

K

L

L

K

L

N

D

S

S

S

Q

V

V

F

L

I

I

G

I

G

G

I

L

G

G

L

L

G

G

G

T

A

P

T

A

A

A

A

L

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

L

V

V

I

L

S

A

H

D

Y

D

G

D

N

D

L

V

T

H

E

L

S

S

N

N

M

L

N

Q

R

R

Q

Q

H

I

L

L

M

F

D

T

V

T

N

E

R

D

I

I

G

D

E

R

P

P

R

K

I

S

D

Q

I

V

A

S

V

Q

E

Q

N

R

I

L

L

T

K

Q

I

L

N

N

P

P

D

D

I

I

E

Q

L

L

E

T

M

A

H

L

N

Q

I

Q

R

R

L

L

N

T

E

G

N

E

K

A

A

L

E

K

K

D

F

A

I

V

E

A

G

R

C

A

D

D

V

V

A

V

V

L

S

D

A

C

R

T

P
x
D

N

N

F

M

P

A

E

T

R

R

M

Q

A

E

M

I

N

N

N

A

A

A

C

C

V

V

K

A

L

L

G

N

V

T

P

P

M

L

I

I

E

T

A

A

A

S

M

-

D

-

M

A

V

V

G

G

Y

F

Y

G

F

G

N

Q

I

L

F

M

-

V

-

L

-

T

-

P

P

P

H

W

E

E

T

Q

A

G

C
|
C

L

Y

R

R

C

C

F

L

V

-

S

-

E

-

D

-

V

W

P

P

D

D

W

N

K

Q

E

E

L

P

G

T

F

A

G

G

V

V

L

V

G

G

A

P

V

V

S

V

G

G

T

V

I

M

G

G

S

T

L

L

A

Q

A

A

V

L

E

E

A

A

V

I

K

K

I

L

T

S

K

G

H

I

G

E

T

T

P

P

L

-

K

A

G

G

K

E

M

L

M

R

H

L

I

F

D

D

F

G

N

K

T

S

M

S

R

Q

T

W

I

R

T

S

P

L

K

A

L

L

K

R

R

R

N

A

P

S

D

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R10), D127 (≠ P126)
binding calcium ion: L14 (≠ M13), D16 (≠ K15)
binding zinc ion: C169 (= C167), C235 (= C238), C238 (= C241)

1zfnA Structural analysis of escherichia coli thif (see paper)
33% identity, 98% coverage: 5:242/244 of query aligns to 6:244/244 of 1zfnA

query
sites
1zfnA

Y

F

E

M

R

R

Y

Y

K

S

R
|
R

Q

Q

I

I

M

L

L

L

K

D

D

D

F

I

G

A

R

L

E

D

K

G

Q

Q

D

Q

I

K

L

L

K

L

N

D

S

S

S

Q

V

V

F

L

I

I

G
x
I

G
|
G

I

L

G
|
G

L

L

G

G

G

T

A

P

T

A

A

A

A

L

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

L

V

V

I

L

S

A

H
x
D

Y

D

G

D

N

D

L

V

T

H

E

L

S

S

N

N

M

L

N

Q

R
|
R

Q
|
Q

H

I

L

L

M

F

D

T

V

T

N

E

R

D

I

I

G

D

E

R

P

P

R
x
K

I

S

D

Q

I

V

A

S

V

Q

E

Q

N

R

I

L

L

T

K

Q

I

L

N

N

P

P

D

D

I

I

E

Q

L

L

E

T

M

A

H

L

N

Q

I

Q

R

R

L

L

N

T

E

G

N

E

K

A

A

L

E

K

K

D

F

A

I

V

E

A

G

R

C

A

D

D

V

V

A

V

V

L

S

D

A

C

R
x
T

P
x
D

N

N

F

M

P

A

E
x
T

R

R

M

Q

A

E

M

I

N

N

N

A

A

A

C

C

V

V

K

A

L

L

G

N

V

T

P

P

M

L

I

I

E

T

A

A

A

S

M

-

D

-

M

A

V

V

G

G

Y

F

Y

G

F

G

N

Q

I

L

F

M

-

V

-

L

-

T

-

P

P

P

H

W

E

E

T

Q

A

G

C
|
C

L

Y

R

R

C
|
C

F

L

V

W

S

P

E

D

D

N

V

Q

-

E

P

P

D

E

W

R

K

N

E

C

L

R

G

T

F

A

G

G

V

V

L

V

G

G

A

P

V

V

S

V

G

G

T

V

I

M

G

G

S

T

L

L

A

Q

A

A

V

L

E

E

A

A

V

I

K

K

I

L

T

S

K

G

H

I

G

E

T

T

P

P

L

-

K

A

G

G

K

E

M

L

M

R

H

L

I

F

D

D

F

G

N

K

T

S

M

S

R

Q

T

W

I

R

T

S

P

L

K

A

L

L

K

R

R

R

N

A

P

S

D

G

C
|
C

P

P

V

V

C
|
C

G

G

active site: R11 (= R10), D127 (≠ P126)
binding adenosine-5'-triphosphate: I34 (≠ G33), G35 (= G34), G37 (= G36), G38 (= G37), D59 (≠ H58), R70 (= R69), Q71 (= Q70), K83 (≠ R82), T126 (≠ R125), D127 (≠ P126), T131 (≠ E130)
binding zinc ion: C169 (= C167), C172 (= C170), C240 (= C238), C243 (= C241)

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
33% identity, 98% coverage: 5:242/244 of query aligns to 6:244/251 of P30138

query
sites
P30138

Y

F

E

M

R

R

Y

Y

K

S

R

R

Q

Q

I

I

M

L

L

L

K

D

D

D

F

I

G

A

R

L

E

D

K

G

Q

Q

D

Q

I

K

L

L

K

L

N

D

S

S

S

Q

V

V

F

L

I

I

G

I

G

G

I

L

G

G

L

L

G

G

G

T

A

P

T

A

A

A

A

L

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

L

V

V

I

L

S

A

H

D

Y

D

G

D

N

D

L

V

T

H

E

L

S

S

N

N

M

L

N

Q

R

R

Q

Q

H

I

L

L

M

F

D

T

V

T

N

E

R

D

I

I

G

D

E

R

P

P

R

K

I

S

D

Q

I

V

A

S

V

Q

E

Q

N

R

I

L

L

T

K

Q

I

L

N

N

P

P

D

D

I

I

E

Q

L

L

E

T

M

A

H

L

N

Q

I

Q

R

R

L

L

N

T

E

G

N

E

K

A

A

L

E

K

K

D

F

A

I

V

E

A

G

R

C

A

D

D

V

V

A

V

V

L

S

D

A

C

R

T

P

D

N

N

F

M

P

A

E

T

R

R

M

Q

A

E

M

I

N

N

N

A

A

A

C

C

V

V

K

A

L

L

G

N

V

T

P

P

M

L

I

I

E

T

A

A

A

S

M

-

D

-

M

A

V

V

G

G

Y

F

Y

G

F

G

N

Q

I

L

F

M

-

V

-

L

-

T

-

P

P

P

H

W

E

E

T

Q

A

G

C
|
C

L

Y

R

R

C
|
C

F

L

V
x
W

S

P

E

D

D

N

V

Q

-

E

P

P

D

E

W

R

K

N

E
x
C

L

R

G

T

F

A

G

G

V

V

L

V

G

G

A

P

V

V

S

V

G

G

T

V

I

M

G

G

S

T

L

L

A

Q

A

A

V

L

E

E

A

A

V

I

K

K

I

L

T

S

K

G

H

I

G

E

T

T

P

P

L

-

K

A

G

G

K

E

M

L

M

R

H

L

I

F

D

D

F

G

N

K

T

S

M

S

R

Q

T

W

I

R

T

S

P

L

K

A

L

L

K

R

R

R

N

A

P

S

D

G

C
|
C

P

P

V

V

C
|
C

G

G

C169 (= C167) binding Zn(2+)
C172 (= C170) binding Zn(2+)
W174 (≠ V172) mutation to A: No adenylation of ThiS.
C184 (≠ E181) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.
C240 (= C238) binding Zn(2+)
C243 (= C241) binding Zn(2+)

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
32% identity, 93% coverage: 7:234/244 of query aligns to 10:217/217 of 1jwaB

query
sites
1jwaB

R

R

Y

Y

K

N

R
|
R

Q

Q

I

I

M

I

L

L

K

R

D

G

F

F

G

D

R

F

E

D

K

G

Q

Q

D

E

I

A

L

L

K

K

N

D

S

S

S

R

V

V

F

L

I

I

G

V

G

G

I

L

G
|
G

L

L

G

G

G

C

A

A

T

A

A

S

A

Q

Y

Y

L

L

A

A

S

S

A

A

G

G

I

V

G

G

R

N

M

L

V

T

I

L

S

L

H
x
D

Y
x
F

G

D

N

T

L

V

T

S

E

L

S

S

N

N

M

L

N

Q

R
|
R

Q

Q

H

T

L

L

M

H

D

S

V

D

N

A

R

T

I

V

G

G

E

Q

P

P

R
x
K

I

V

D

E

I

S

A

A

V

R

E

D

N

A

I

L

L

T

K

R

I

I

N

N

P

P

D

H

I

I

E

A

L

I

E

T

M

P

H

V

N

N

I

A

R
x
L

L

L

N

D

E

D

N

A

K

E

A

L

E

A

K

A

F

L

I

I

E

A

G

E

C

H

D

D

V

L

A

V

V

L

S

D

A

C

R

T

P
x
D

N
|
N

F

V

P

A

E
x
V

R

R

M

N

A

Q

M

L

N

N

N

A

A

G

C

C

V

F

K

A

L

A

G

K

V

V

P

P

M

L

I

V

E

S

A

G

A

A

M

A

D

I

D

R

M

M

V

E

G

G

Y

-

Y

Q

F

I

N

T

I

V

F

F

P

T

H

Y

E

E

T

A

A

-

C

-

L

-

R

-

C

-

F

-

V

-

S

-

E

-

D

-

V

-

P

-

D

-

W

-

K

-

E

-

L

-

G

-

F

-

G

G

V

V

L

M

G

A

A

P

V

L

S

I

G

G

T

V

I

I

G

G

S

S

L

L

A

Q

A

A

V

M

E

E

A

A

V

I

K

K

I

M

I

L

T

A

K

G

H

Y

G

G

T

K

P

P

L

A

K

S

G

G

K

K

M

I

M

V

H

M

I

Y

D

D

F

A

N

M

T

T

M

C

R

Q

T

F

I

R

T

E

P

M

K

K

L

L

K

M

R

R

active site: R13 (= R10), D129 (≠ P126)
binding adenosine-5'-triphosphate: G39 (= G36), G40 (= G37), D61 (≠ H58), F62 (≠ Y59), R72 (= R69), K85 (≠ R82), L108 (≠ R105), D129 (≠ P126), N130 (= N127), V133 (≠ E130)

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
29% identity, 97% coverage: 5:241/244 of query aligns to 66:318/482 of O59954

query
sites
O59954

Y

Y

E

R

R

R

Y

Y

K

G

R

R

Q

Q

I

M

M

I

L

V

K

P

D

Q

F

F

G

G

R

L

E

Q

K
x
G

Q

Q

D

L

I

K

L

L

K

R

N

D

S

A

S

K

V

V

F

L

I

I

G

V

G

G

I

A

G

G

L

L

G
|
G

G

C

A

P

T

A

A

A

A

L

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

I

V

G

I

L

S

V

H

D

Y

G

G

D

N

T

L

V

T

E

E

A

S

S

N

N

M

L

N

H

R
|
R

Q

Q

H

V

L

L

M

H

D

R

V

S

N

R

R

N

I

V

G

G

E

K

P

L

R

K

I

V

D

D

I

S

A

A

V

I

E

E

N

Y

I

L

L

R

K

E

I

L

N

N

P

P

D

H

I

P

E

T

L

Y

E

I

M

A

H

H

N

Q

I

A

R

H

L

L

N

T

E

P

N

R

K

E

A

A

E

P

K

D

F

I

I

F

E

K

G

D

C

Y

D

D

V

L

A

I

V

L

S

D

A
x
C

R

T

P

D

N

N

F

P

P

A

E

T

R

R

M

Y

A

L

M

I

N

S

N

D

A

T

C

A

V

V

K

L

L

L

G

G

V

K

P

P

M

L

I

V

E

S

A

A

A

S

M

A

D

L

D

R

M

T

V
x
E

G

G

Y

Q

Y

L

-

M

-

V

F

L

N

N

I

N

F

P

P

P

H

Q

E

P

T

P

A

G

-

D

-

K

-

T

-

G

-

P

C

C

L

Y

R

R

C

C

F

V

V

F

S

P

E

K

D

P

V

P

P

P

D

A

W

N

K

S

E

V

L

T

G

S

F

C

-

A

-

D

-

G

G

G

V

I

L

L

G

G

A

P

V

V

S

V

G
|
G

T

T

I

M

G

G

S

V

L

L

A

Q

A

A

V

S

E

E

A

A

V

I

K

K

I

V

I

L

T

T

K

S

H

A

G

G

T

E

P

S

L

V

K

E

G

A

K

T

-

P

-

S

-

L

M

L

M

I

H

F

I

S

D

A

F

Y

N

S

T

S

M

P

R

Q

T

F

I

R

T

T

P

I

K

K

L

L

K

R

-

S

R

R

N

R

P

P

D

N

C

C

P

A

V

V

C

C

G82 (≠ K21) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G39) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R69) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ A124) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ V154) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G192) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
30% identity, 91% coverage: 6:226/244 of query aligns to 13:234/289 of 6yubB

query
sites
6yubB

E

E

R

R

Y

Y

K

S

R

R

Q

Q

I

M

M

I

L

V

K

P

D

G

F

M

G

G

R

K

E

E

K

G

Q

Q

D

L

I

R

L

L

K

M

N

N

S

A

S

K

V

V

F

L

I

I

G

I

G

G

I

A

G

G

L

L

G

G

G

C

A

P

T

A

A

A

A

Q

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

I

V

G

I

I

S

V

H

D

Y

G

G

D

N

S

L

V

T

E

E

T

S

S

N

N

M

L

N

H

R

R

Q

Q

H

V

L

A

M

H

D

A

V

T

N

K

R

R

I

V

G

G

E

M

P

L

R

K

I

V

D

D

I

S

A

L

V

I

E

T

N

H

I

L

L

I

K

E

I

I

N

N

P

P

D

L

I

P

E

V

L

Y

E

V

M

P

H

Y

N

R

I

F

R

D

L

L

N

T

E

P

N

Q

K

N

A

A

E

A

K

Q

F

I

I

I

E

K

G

P

C

W

D

D

V

V

A

I

V

L

S

D

A

C

R

T

P

D

N

N

F

P

P

A

E

T

R

R

M

Y

A

L

M

I

N

S

N

D

A

V

C

C

V

V

K

L

L

L

G

G

V

K

P

P

M

L

I

V

E

S

A

A

M

S

D

V

D

Q

M

K

V

S

G

G

Y

Q

Y

L

-

I

-

V

F

L

N

N

I

C

F

P

P

P

H

T

E

P

T

Q

A

G

C

V

L

V

R

N

C

-

F

-

V

-

S

K

E

K

D

A

V

A

P

P

D
x
C

W

Y

K

R

E
x
C

L

C

G

F

F

K

G

G

V

I

L

M

G

G

A

P

V

V

S

V

G

G

T

M

I

M

G

G

S

V

L

A

A

Q

A

A

V

G

E

E

A

A

V

I

K

K

I

I

I

L

T

V

K

S

-

Q

-

L

H

H

G

M

T

P

P

P

L

K

K

E

G

G

K

E

M

E

M

V

H

S

I

P

D

E

F

K

N

N

T

L

M

V

R

Q

binding zinc ion: C184 (≠ D178), C187 (≠ E181)

Sites not aligning to the query:

binding zinc ion: 265

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
29% identity, 98% coverage: 5:243/244 of query aligns to 44:291/440 of P38820

query
sites
P38820

Y

Y

E

Q

R

R

Y

Y

K

G

R

R

Q

Q

I

M

M

I

L

V

K

E

D

E

F

T

G

G

R

G

E

V

K

A

Q

G

D

Q

I

V

-

K

L

L

K

K

N

N

S

T

S

K

V

V

F

L

I

V

G

V

G

G

I

A

G

G

L

L

G

G

G

C

A

P

T

A

A

L

A

P

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

Q

M

I

V

G

I

I

S

V

H

D

Y

N

G

D

N

V

L

V

T

E

E

T

S

S

N

N

M

L

N

H

R

R

Q

Q

H

V

L

L

M

H

D

D

V

S

N

S

R

R

I

V

G

G

E

M

P

L

R

K

I

C

D

E

I

S

A

A

V

R

E

Q

N

Y

I

I

L

T

K

K

I

L

N

N

P

P

D

H

I

I

E

N

L

V

E

V

M

T

H

Y

N

P

I

V

R

R

L

L

N

N

E

S

N

S

K

N

A

A

E

F

K

D

F

I

I

F

E

K

G

G

C

Y

D

N

V

Y

A

I

V

L

S

D

A

C

R

T

P

D

N

S

F

P

P

L

E

T

R

R

M

Y

A

L

M

V

N

S

N

D

A

V

C

A

V

V

K

N

L

L

G

G

V

I

P

T

M

V

I

V

E

S

A

A

A

S

M

G

D

L

D

G

M

T

V

E

G

G

Y

Q

-

L

-

T

-

I

-

L

-

N

Y

F

F

N

N

N

I

I

F

G

P

P

H

-

E

-

T

-

A

-

C

C

L

Y

R

R

C

C

F

F

V

Y

S

P

E

T

D

P

V

P

P

P

D

P

W

N

K

A

E

V

L

T

G

S

F
x
C

-

Q

-

E

-

G

G

G

V

V

L

I

G

G

A

P

V

C

S

I

G

G

T

L

I

V

G

G

S

T

L

M

A

M

A

A

V

V

E

E

A

T

V

L

K

K

I

L

I

I

-

L

-

G

-

I

-

Y

-

T

T

N

K

E

H

N

G

F

T

S

P

P

L

F

K

-

G

-

K

L

M

M

M

L

H

Y

I

S

D

G

F

F

N

P

T

Q

M

Q

R

S

T

L

I

R

T

T

P

F

K

K

L

M

K

R

-

G

R

R

N

Q

P

E

D

K

C

C

P

L

V

C

C

C

G

G

R

K

C225 (≠ F184) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
30% identity, 91% coverage: 6:226/244 of query aligns to 12:235/423 of 6yubA

query
sites
6yubA

E

E

R

R

Y

Y

K

S

R

R

Q

Q

I

M

M

I

L

V

K

P

D

G

F

M

G

G

R

K

E

E

K

G

Q

Q

D

L

I

R

L

L

K

M

N

N

S

A

S

K

V

V

F

L

I

I

G

I

G

G

I

A

G

G

L

L

G

G

G

C

A

P

T

A

A

A

A

Q

Y

Y

L

L

A

A

S

G

A

A

G

G

I

V

G

G

R

T

M

I

V

G

I

I

S

V

H

D

Y

G

G

D

N

S

L

V

T

E

E

T

S

S

N

N

M

L

N

H

R

R

Q

Q

H

V

L

A

M

H

D

A

V

T

N

K

R

R

I

V

G

G

E

M

P

L

R

K

I

V

D

D

I

S

A

L

V

I

E

T

N

H

I

L

L

I

K

E

I

I

N

N

P

P

D

L

I

P

E

V

L

Y

E

V

M

P

H

Y

N

R

I

F

R

D

L

L

N

T

E

P

N

Q

K

N

A

A

E

A

K

Q

F

I

I

I

E

K

G

P

C

W

D

D

V

V

A

I

V

L

S

D

A

C

R

T

P

D

N

N

F

P

P

A

E

T

R

R

M

Y

A

L

M

I

N

S

N

D

A

V

C

C

V

V

K

L

L

L

G

G

V

K

P

P

M

L

I

V

E

S

A

A

M

S

D

V

D

Q

M

K

V

S

G

G

Y

Q

Y

L

-

I

-

V

F

L

N

N

I

C

F

P

P

P

-

T

-

P

-

Q

-

G

-

V

-

N

-

K

H

K

E

A

T

A

A

P

C
|
C

L

Y

R

R

C
|
C

F

C

V

F

S

K

E

K

D

P

V

-

P

-

D

-

W

-

K

-

E

-

L

-

G

-

F

-

G

G

V

I

L

M

G

G

A

P

V

V

S

V

G

G

T

M

I

M

G

G

S

V

L

A

A

Q

A

A

V

G

E

E

A

A

V

I

K

K

I

I

I

L

T

V

K

S

-

Q

-

L

H

H

G

M

T

P

P

P

L

K

K

E

G

G

K

E

M

E

M

V

H

S

I

P

D

E

F

K

N

N

T

L

M

V

R

Q

binding zinc ion: C183 (= C167), C186 (= C170)

Sites not aligning to the query:

binding zinc ion: 266, 269

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
30% identity, 98% coverage: 7:244/244 of query aligns to 62:303/460 of O95396

query
sites
O95396

R

R

Y

Y

K

S

R

R

Q

Q

I

L

M

V

L

L

K

P

D

E

F

L

G

G

R

V

E

H

K

G

Q

Q

D

L

I

R

L

L

K

G

N

T

S

A

S

C

V

V

F

L

I

I

G

V

G

G

I

C

G

G

L

L

G

G

G

C

A

P

T

L

A

A

A

Q

Y

Y

L

L

A

A

S

A

A

A

G

G

I

V

G

G

R

R

M

L

V

G

I

L

S

V

H

D

Y

Y

G

D

N

V

L

V

T

E

E

M

S

S

N

N

M

L

N

A

R

R

Q

Q

H

V

L

L

M

H

D

G

V

E

N

A

R

L

I

A

G

G

E

Q

P

A

R

K

I

A

D

F

I

S

A

A

V

A

E

A

N

S

I

L

L

R

K

R

I

L

N

N

P

S

D

A

I

V

E

E

L

C

E

V

M

P

H

Y

N
x
T

I
x
Q

R
x
A

L
|
L

N
x
T

E
x
P

N
x
A

K
x
T

A
|
A

E
x
L

K
x
D

F
x
L

I
x
V

E
x
R

G
x
R

C
x
Y

D
|
D

V
|
V

A
x
V

V
x
A

S
x
D

A
x
C

R
x
S

P
x
D

N
|
N

F
x
V

P
|
P

E
x
T

R
|
R

M
x
Y

A
x
L

M
x
V

N
|
N

N
x
D

A
|
A

C
|
C

V
|
V

K
x
L

L
x
A

G
|
G

V
x
R

P
|
P

M
x
L

I
x
V

E
x
S

A
|
A

A
x
S

M
x
A

D
x
L

D
x
R

M
x
F

V
x
E

G
|
G

-
x
Q

-
x
I

-
x
T

-
x
V

Y
|
Y

Y
x
H

F
x
Y

N
x
D

I
x
G

F
x
G

P
|
P

H

-

E

-

T

-

A

-

C
|
C

L
x
Y

R
|
R

C
|
C

F
x
I

V
x
F

S
x
P

E
x
Q

D
x
P

V
x
P

P
|
P

D
x
A

W
x
E

K
x
T

E
x
V

L
x
T

G
x
N

F
x
C

G

A

-

D

-

G

V

V

L

L

G

G

A

V

V

V

S

T

G

G

T

V

I

L

G

G

S

C

L

L

A

Q

A

A

V

L

E

E

A

V

V

L

K

K

I

I

I

A

T

A

K

G

H

L

G

G

T

P

P

S

L

Y

K

S

G

G

K

S

M

L

H

L

I

-

D

-

F

F

N

D

T

A

M

L

R

R

-

G

-

H

-

F

T

R

I

S

T

I

P

R

K

L

L

R

K

S

R

R

N

R

P

L

D

D

C

C

P

A

V

A

C

C

G

G

R

E

R

R

158:238 (vs. 103:183, 25% identical) Interaction with NFS1
C239 (≠ F184) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

Q8VE47 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; UFM1-activating enzyme from Mus musculus (Mouse) (see 2 papers)
27% identity, 95% coverage: 8:238/244 of query aligns to 51:301/403 of Q8VE47

query
sites
Q8VE47

Y

Y

K

S

R

R

Q

L

I

M

M

A

L

L

K

K

D

R

F

M

G

G

-

I

R

V

E

S

K

D

Q

Y

D

K

I

K

L

I

K

R

N

T

S

Y

S

A

V

V

F

A

I

I

G

V

G

G

I

V

G

G

L

V

G

G

G

S

A

V

T

T

A

A

A

E

Y

M

L

L

A

T

S

R

A

C

G

G

I

I

G

G

R

K

M

L

V

L

I

L

S

F

H

D

Y

Y

G

D

N

K

L

V

T

E

E

L

S

A

N

N

M

M

N

N

R

R

Q

L

H

F

L

F

M

Q

D

P

V

Y

N

-

R

Q

I

A

G

G

E

L

P

S

R

K

I

V

D

H

I

A

A

A

V

E

E

H

N

T

I

L

L

R

K

N

I

I

N

N

P

P

D

D

I

V

E

L

L

F

E

E

M

V

H

H

N

N

I

Y

R

N

L

I

N

T

E

T

-

V

N

E

K

H

A

F

E

E

K

H

F

F

I

M

-

N

-

R

-

I

-

S

-

N

-

G

-

L

-

E

-

G

E

Q

G

P

C

V

D

D

V

L

A

V

V

L

S

S

A

C

R

V

P

D

N

N

F

F

P

E

E

A

R

R

M

M

A

A

M

I

N

N

N

T

A

A

C

C

V

N

K

E

L

L

G

G

V

Q

P

T

M

W

I

M

E

E

A

S

A

G

M

V

D

S

D

E

-

N

-

A

M

V

V

S

G

G

Y

H

Y

I

F

Q

N

L

I

M

F

I

P

P

H

G

E

E

T

S

A

A

C

C

L

F

R

A

C

C

-

A

-

P

-

L

F

V

V

V

S

A

E

S

D

N

V

I

P

D

D

E

W

K

K

T

E

L

L

K

G

R

F

E

G

G

V

V

L
x
C

G

A

A

A

-

S

V

L

S

P

G

T

T

T

I

M

G

G

S

V

L

V

A

A

A

G

V

I

-

L

-

V

-

Q

E

N

A

V

V

L

K

K

I

F

I

L

T

L

K

K

H

F

G

G

T

T

P

-

L

-

K

-

G

-

K

V

M

S

M

F

H

Y

I

L

D

G

F

Y

N

N

T

A

M

M

R

Q

T

D

I

F

T

F

P

P

K

T

-

M

-

F

L

M

K

K

R

P

N

N

P

P

D

Q

C

C

C248 (≠ L187) mutation C->S,A: Abolished ability to catalyze the first step in ufmylation.

Sites not aligning to the query:

340:344 WGIEL→LGIEA: Impaired ability to activate UFM1.

Q06624 DNA damage tolerance protein RHC31; RAD31 homolog from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
28% identity, 71% coverage: 2:175/244 of query aligns to 10:173/347 of Q06624

query
sites
Q06624

H

E

D

D

Y

E

Y

I

E

A

R

L

Y

Y

K

D

R

R

Q

Q

I

I

M

R

L

L

K

-

D

-

F

W

G

G

R

M

E

T

K

A

Q

Q

D

A

I

N

L

M

K

R

N

S

S

A

S
x
K

V

V

F

L

I

L

G

I

G

N

I

L

G

G

G

A

L

I

G

G

G

S

A

E

T

I

A

T

A

K

Y

S

L

I

A

V

S

L

A

S

G

G

I

I

G

G

R

H

M

L

V

T

I

I

S

L

H

D

Y

G

G

H

N

M

L

V

T

T

E

E

S

E

N

D

M

L

N

G

R

S

Q

Q

H

F

L

F

M

I

D

G

V

S

N

E

R

D

I

V

G

G

E

Q

P

W

R

K

I

I

D

D

I

A

A

T

V

K

E

E

N

R

I

I

L

Q

K

D

I

L

N

N

P

P

D

R

I

I

E

E

L

L

E

N

M

F

H

D

N

K

I

Q

R

D

L

L

N

Q

E

E

N

-

K

K

A

D

E

E

K

E

F

F

I

F

E

Q

G

Q

C

F

D

D

V

L

A

V

V

V

S

A

A

T

R

E

P

M

N

Q

F

I

P

D

E

E

R

A

M

I

A

K

M

I

N

N

N

T

A

L

C

T

V

R

K

K

L

L

G

N

V

I

P

P

M

L

I

Y

E

V

A

A

A

G

M

S

D

N

D

G

M

L

V

F

G

A

Y

Y

Y

V

F

F

N

-

I

-

F

-

P

-

H

-

E

-

T

-

A

I

C

D

L

L

R

I

C

E

F

F

V

I

S

S

E

E

D

D

K35 (≠ S29) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)

6h77B E1 enzyme for ubiquitin like protein activation in complex with ubl (see paper)
27% identity, 97% coverage: 8:244/244 of query aligns to 18:271/302 of 6h77B

query
sites
6h77B

Y

Y

K

S

R

R

Q

L

I

M

M

A

L

L

K

K

D

R

F

M

G

G

-

I

R

V

E

S

K

D

Q

Y

D

E

I

K

L

I

K

R

N

T

S

F

S

A

V

V

F

A

I

I

G

V

G
|
G

I

V

G
|
G

L

V

G

G

G

S

A

V

T

T

A

A

A

E

Y

M

L

L

A

T

S

R

A

C

G

G

I

I

G

G

R

K

M

L

V

L

I

L

S

F

H
x
D

Y
|
Y

G
x
D

N

K

L

V

T

E

E

L

S

A

N
|
N

M

M

N

N

R
|
R

Q

L

H

-

L

F

M

F

D

Q

V

P

N

H

R

Q

I

A

G

G

E

L

P

S

R
x
K

I

V

D

Q

I

A

A

A

V

E

E

H

N

T

I

L

L

R

K

N

I

I

N

N

P

P

D

D

I

V

E

L

L

F

E

E

M

V

H

H

N

N

I

-

R

-

L

Y

N
|
N

E
x
I

N

T

K

T

A

V

E

E

K

N

F

F

I

Q

-

H

-

F

-

M

-

D

-

R

-

I

-

S

-

N

-

G

-

L

-

E

E

E

G

G

-

K

-

P

C

V

D

D

V

L

A

V

V

L

S

S

A

C

R

V

P
x
D

N
|
N

F

F

P

E

E

A

R

R

M

M

A

T

M

I

N

N

N

T

A

A

C

C

V

N

K

E

L

L

G

G

V

Q

P

T

M

W

I

M

E

E

A

S

A

G

M

V

D

S

D

E

-

N

-

A

M

V

V

S

G

G

Y

H

Y

I

F

Q

N

L

I

I

F

I

P

P

H

G

E

E

T

S

A

A

C
|
C

L

F

R

A

C
|
C

-

A

-

P

-

L

F

V

V

V

S

A

E

A

D

N

V

I

P

D

D

E

W

K

K

T

E

L

L

K

G

R

F

E

G

G

V

V

L

C

G

A

A

A

-

S

V

L

S

P

G

T

T

T

I

M

G

G

S

V

L

V

A

A

A

G

V

I

-

L

-

V

-

Q

E

N

A

V

V

L

K

K

I

F

I

L

T

L

K

N

H

F

G

G

T

T

P

-

L

-

K

-

G

-

K

V

M

S

M

F

H

Y

I

L

D

G

F

Y

N

N

T

A

M

M

R

Q

T

D

I

F

T

F

P

P

K

T

L

M

K

S

R

M

N

K

P

P

D

N

C

-

P

P

V

Q

C
|
C

G

D

R

D

R

R

binding adenosine-5'-triphosphate: G45 (= G34), G47 (= G36), G48 (= G37), D69 (≠ H58), Y70 (= Y59), D71 (≠ G60), N77 (= N66), R80 (= R69), K92 (≠ R82), N115 (= N107), I116 (≠ E108), D148 (≠ P126), N149 (= N127)
binding zinc ion: C191 (= C167), C194 (= C170), C268 (= C241)

Sites not aligning to the query:

binding zinc ion: 273

Q9GZZ9 Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1 from Homo sapiens (Human) (see 13 papers)
28% identity, 95% coverage: 8:238/244 of query aligns to 53:303/404 of Q9GZZ9

query
sites
Q9GZZ9

Y

Y

K

S

R
|
R

Q

L

I
x
M

M

A

L

L

K

K

D

R

F

M

G

G

-

I

R

V

E

S

K

D

Q

Y

D

E

I

K

L

I

K

R

N

T

S

F

S

A

V

V

F

A

I

I

G

V

G

G

I

V

G

G

G
|
G

L

V

G

G

G

S

A

V

T

T

A

A

A

E

Y

M

L

L

A

T

S

R

A

C

G

G

I

I

G

G

R

K

M

L

V

L

I

L

S

F

H
x
D

Y

Y

G

D

N

K

L

V

T

E

E

L

S

A

N

N

M

M

N

N

R

R

Q

-

H

L

L

F

M

F

D

Q

V

P

N

H

R

Q

I

A

G

G

E

L

P

S

R
x
K

I

V

D

Q

I

A

A

A

V

E

E

H

N

T

I

L

L

R

K

N

I

I

N

N

P

P

D

D

I

V

E

L

L

F

E

E

M

V

H

H

N

N

I

-

R

-

L

Y

N
|
N

E

I

N

T

K

T

A

V

E

E

K

N

F

F

I

Q

-

H

-

F

-

M

-

D

-

R

-

I

-

S

-

N

-

G

-
x
G

-

L

-

E

E

E

G

G

-

K

-

P

C

V

D

D

V

L

A

V

V

L

S

S

A

C

R

V

P

D

N
|
N

F

F

P

E

E

A

R
|
R

M

M

A

T

M

I

N

N

N

T

A

A

C

C

V

N

K

E

L

L

G

G

V

Q

P

T

M

W

I

M

E

E

A

S

A

G

M

V

D

S

D

E

-

N

-

A

M

V

V

S

G

G

Y
x
H

Y
x
I

F
x
Q

N

L

I

I

F

I

P

P

H

G

E

E

T

S

A

A

C
|
C

L

F

R

A

C
|
C

-
x
A

-
x
P

-
x
L

F

V

V

V

S

A

E

A

D

N

V

I

P

D

D

E

W

K

K

T

E

L

L

K

G
x
R

F
x
E

G
|
G

V
|
V

L
x
C

G
x
A

A
|
A

-
x
S

V
x
L

S
x
P

G
x
T

T
|
T

I
x
M

G
|
G

S
x
V

L
x
V

A
|
A

A
x
G

V
x
I

-
x
L

-
x
V

-
x
Q

E
x
N

A
x
V

V
x
L

K
|
K

I
x
F

I
x
L

T
x
L

K
x
N

H
x
F

G
|
G

T
|
T

P

-

L

-

K

-

G

-

K
x
V

M
x
S

M
x
F

H
x
Y

I
x
L

D
x
G

F
x
Y

N
|
N

T
x
A

M
|
M

R
x
Q

T
x
D

I
x
F

T
x
F

P
|
P

-
x
T

-
x
M

K
x
S

L
x
M

K
|
K

R
x
P

N
|
N

P
|
P

D
x
Q

C
|
C

R55 (= R10) to H: in DEE44; reduces UFM1 activating enzyme activity; dbSNP:rs774318611
M57 (≠ I12) to V: in DEE44; reduces UFM1 activating enzyme activity; reduces UFM1-DDRGK1 formation; dbSNP:rs532178791
G83 (= G37) binding ATP
D104 (≠ H58) binding ATP
K127 (≠ R82) binding ATP
N150 (= N107) binding ATP
G168 (vs. gap) to E: in DEE44; abolishes UFM1 activating enzyme activity; dbSNP:rs886039761
N184 (= N127) binding ATP
R188 (= R131) mutation to A: Abolished ability to activate UFM1.
HIQ 215:217 (≠ YYF 156:158) mutation to AQA: Abolished ability to activate UFM1.
C226 (= C167) binding Zn(2+)
C229 (= C170) binding Zn(2+)
APPL 230:233 (vs. gap) mutation APPL->RPPA,FPPA: Abolished interaction with UFM1.
C250 (≠ L187) active site, Glycyl thioester intermediate; mutation to A: Abolished ability to activate UFM1.; mutation to S: Forms a stable intermediate complex.
V260 (≠ S196) to M: in DEE44; reduces UFM1 activating enzyme activity; dbSNP:rs886039759
K271 (= K204) mutation to D: Impaired ability to activate UFM1 via a trans-binding mechanism.
D290 (≠ T227) mutation to K: Impaired homodimerization and ability to activate UFM1 via a trans-binding mechanism.
C303 (= C238) binding Zn(2+)

Sites not aligning to the query:

246:404 natural variant: Missing (in SCAR24; delocalizes protein to the nucleus; activates degradation through the ubiquitin proteasome pathway; decreases protein stability; disrupts interaction with UFM1)
308 binding Zn(2+)
310 K → E: in SCAR24; does not affect cytoplasm localization; decreases protein stability; does not affect interaction with UFM1; dbSNP:rs886039762
334:346 UFM1-interacting sequence (UIS)
336 mutation H->A,D: Impaired ability to activate UFM1.
341 W→A: Abolished interaction with UFM1 and GABARAPL2.
342 G→A: Decreased interaction with UFM1 without affecting interaction with GABARAPL2.
343 I→A: Abolished interaction with UFM1 and decreased interaction with GABARAPL2.
343:345 IEL→AEA: Impaired ability to activate UFM1.
345 L→A: Abolished interaction with UFM1 and decreased interaction with GABARAPL2.
346 V→A: Abolished interaction with UFM1 and decreased interaction with GABARAPL2.
347:377 Linker
371 A → T: in DEE44; reduces UFM1 activating enzyme activity; reduces UFM1 activating enzyme activity; reduces UFM1-DDRGK1 formation; dbSNP:rs114925667
372 mutation Y->A,E: Strongly decreased ability to transfer UFM1 to UFC1.; Y→F: Does not affect ability to transfer UFM1 to UFC1.
389 D → Y: in DEE44; no effect on UFM1 activating enzyme activity; dbSNP:rs886039760
389:404 UFC1-binding sequence (UFC)
397 L→R: Abolished interaction with UFC1.
401 M→R: Abolished interaction with UFC1.

P20973 Ubiquitin-activating enzyme E1 1; EC 6.2.1.45 from Triticum aestivum (Wheat) (see paper)
31% identity, 58% coverage: 7:148/244 of query aligns to 444:592/1051 of P20973

query
sites
P20973

R

R

Y

Y

K

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Sites not aligning to the query:

626 active site, Glycyl thioester intermediate; C→A: Abolishes enzyme activity.

Query Sequence

>WP_013010412.1 NCBI__GCF_000025725.1:WP_013010412.1
MHDYYERYKRQIMLKDFGREKQDILKNSSVFIGGIGGLGGATAAYLASAGIGRMVISHYG
NLTESNMNRQHLMDVNRIGEPRIDIAVENILKINPDIELEMHNIRLNENKAEKFIEGCDV
AVSARPNFPERMAMNNACVKLGVPMIEAAMDDMVGYYFNIFPHETACLRCFVSEDVPDWK
ELGFGVLGAVSGTIGSLAAVEAVKIITKHGTPLKGKMMHIDFNTMRTITPKLKRNPDCPV
CGRR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory