SitesBLAST

Q9M8S8 Inositol-phosphate phosphatase; L-galactose 1-phosphate phosphatase; Myo-inositol monophosphatase; EC 3.1.3.25; EC 3.1.3.93 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
39% identity, 84% coverage: 13:227/255 of query aligns to 22:244/271 of Q9M8S8

query
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Q9M8S8

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P92 (= P79) mutation to L: In vtc4-1; low levels of ascorbate.

3luzA Crystal structure of extragenic suppressor protein suhb from bartonella henselae, via combined iodide sad molecular replacement (see paper)
37% identity, 97% coverage: 7:253/255 of query aligns to 9:236/238 of 3luzA

query
sites
3luzA

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active site: E54 (= E62), D71 (= D78), L73 (≠ I80), D74 (= D81), T76 (= T83), D191 (= D207)
binding iodide ion: F49 (≠ Y57), L197 (≠ V213), R200 (≠ G216)
binding magnesium ion: E54 (= E62), D71 (= D78), L73 (≠ I80)

Sites not aligning to the query:

binding iodide ion: 3

3luzB Crystal structure of extragenic suppressor protein suhb from bartonella henselae, via combined iodide sad molecular replacement (see paper)
38% identity, 97% coverage: 7:253/255 of query aligns to 11:232/234 of 3luzB

query
sites
3luzB

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active site: E55 (= E62), D69 (= D78), L71 (≠ I80), D72 (= D81), T74 (= T83), D187 (= D207)
binding iodide ion: F50 (≠ Y57), T111 (≠ I120), E113 (= E122), R196 (≠ G216), F201 (≠ H221), V202 (= V222)
binding magnesium ion: E55 (= E62), D69 (= D78), L71 (≠ I80)

Sites not aligning to the query:

binding iodide ion: 5

6ib8B Structure of a complex of suhb and nusa ar2 domain (see paper)
35% identity, 96% coverage: 1:245/255 of query aligns to 8:253/270 of 6ib8B

query
sites
6ib8B

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binding magnesium ion: D88 (= D78), L90 (≠ I80)

P0ADG4 Nus factor SuhB; Inositol-1-monophosphatase; I-1-Pase; IMPase; Inositol-1-phosphatase; EC 3.1.3.25 from Escherichia coli (strain K12) (see 5 papers)
35% identity, 96% coverage: 1:245/255 of query aligns to 4:249/267 of P0ADG4

query
sites
P0ADG4

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E67 (= E62) binding Mg(2+)
D84 (= D78) binding Mg(2+)
L86 (≠ I80) binding Mg(2+)
D87 (= D81) mutation to N: Loss of IMPase activity, still complements dnaB121 helicase mutation.
R139 (≠ K133) mutation to C: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with A-250.
G173 (= G168) mutation to V: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNA polymerase (RNAP).
R183 (= R178) mutation to A: Some growth at 30 degrees Celsius, greater IMPase activity, partially inhibited by RNAP.
R184 (= R179) mutation to A: Grows at 30 degrees Celsius, makes more dimer, partially inhibited by RNAP, crystallizes.; mutation to I: No growth at 30 degrees Celsius, IMPase activity not inhibited by RNAP.

Sites not aligning to the query:

250 V→A: In suhB10; decreases polypeptide chain elongation rate, grows at 30 but not 25 degrees Celsius; when associated with C-139.
251:254 KAML→AAMA: 3-fold decreased affinity for NusA, less efficient in delaying or suppressing Rho-dependent transcription termination.

2qflA Structure of suhb: inositol monophosphatase and extragenic suppressor from e. Coli (see paper)
34% identity, 96% coverage: 1:245/255 of query aligns to 4:249/262 of 2qflA

query
sites
2qflA

M

M

L

L

S

N

K

I

I

A

E

V

K

R

A

A

V

A

T

R

K

K

A

A

G

G

E

N

H

L

L

I

L

A

K

K

G

N

F

Y

N

E

S

T

-

P

-

D

V

A

K

V

E

E

I

A

S

S

Y

Q

K

K

G

G

K

S

I

N

D

D

I

F

V

V

T

T

Q

N

F

V

D
|
D

V

K

E

A

V

A

E

E

K

A

I

V

L

I

N

D

E

T

L

I

S

R

F

K

M

S

M

Y

P

P

E

Q

Y

H

G

T

F

I

V

I

A

T

E
|
E

E

E

T

S

A

G

Q

E

-

L

Q

E

K

G

T

T

D

D

K

Q

K

D

K

V

V

Q

V

W

Y

V

I

I

D
|
D

P

P

I
x
L

D
|
D

G

G

T
|
T

T

T

N

N

F

F

V

I

H

K

G

R

F

L

P

P

F

H

C

F

C

A

V

V

S

S

V

I

G

A

V

V

Y

R

E

I

E

K

G

G

E

R

G

T

I

E

Y

V

G

A

V

V

I

V

Y

Y

N

D

P

P

I

M

L

R

R

N

E

E

M

L

F

F

I

T

A

A

E

T

K

R

G

G

Q

Q

G

G

A

A

Y

Q

L

L

N

N

R

G

Q

Y

K

R

I

L

Q

R

V

G

S

S

S

T

K

A

H

R

N

D

I

L

Q

D

E

G

A

T

L

I

V

L

A

A

T

T

G

G

F

F

P

P

Y

F

S

K

I

-

A

A

E

K

G

Q

N

Y

P

A

Q

T

A

T

A

Y

L

I

E

N

L

I

L

V

G

G

T

K

I

L

L

F

K

N

N

E

T

C

R

A

G

D

I

F

R

R

R

A

A

T

G
|
G

S
|
S

A
|
A

A

A

L

L

D

D

L

L

C

A

Y

Y

V

V

A

A

K

A

G

G

V

R

F

V

D

D

A

G

Y

F

E

E

F

I

D

G

L

L

S

R

P

P

W

W

D
|
D

I

F

A

A

G

G

A

E

V

L

I

L

A

V

G

R

E

E

A

A

E

G

A

G

H

I

V

V

C

S

G

-

K

-

D

D

F

F

S

T

K

G

S

G

P

H

D

N

-

Y

M

M

Y

L

E

T

K

G

F

N

I

I

M

V

A

A

V

G

T

N

P

P

D

R

I

V

active site: D44 (= D39), E67 (= E62), D84 (= D78), L86 (≠ I80), D87 (= D81), T89 (= T83), D212 (= D207)
binding ethyl acetate: D87 (= D81), G186 (= G181), S187 (= S182), A188 (= A183)

6tqoT Rrn anti-termination complex (see paper)
33% identity, 96% coverage: 1:245/255 of query aligns to 4:241/255 of 6tqoT

query
sites
6tqoT

M

M

L

L

S

N

K

I

I

A

E

V

K

R

A

A

V

A

T

R

K

K

A

A

G

G

E

N

H

L

L

I

L

A

K

K

G

N

F

Y

N

E

S

T

V

-

K

-

E

-

I

-

S

-

Y

-

K

P

G

D

K

A

I

N

D

D

I

F

V

V

T

T

Q

N

F

V

D

D

V

K

E

A

V

A

E

E

K

A

I

V

L

I

N

D

E

T

L

I

S

R

F

K

M

S

M

Y

P

P

E

Q

Y

H

G

T

F

I

V

I

A

T

E
|
E

E

E

T

S

A

G

Q

E

-

L

Q

E

K

G

T

T

D

D

K

Q

K

D

K

V

V

Q

V

W

Y

V

I

I

D
|
D

P

P

I
x
L

D

D

G

G

T

T

N

N

F

F

V

I

H

K

G

R

F

L

P

P

F

H

C

F

C

A

V

V

S

S

V

I

G

A

V

V

Y

R

E

I

E

K

G

G

E

R

G

T

I

E

Y

V

G

A

V

V

I

V

Y

Y

N

D

P

P

I

M

L

R

R

N

E

E

M

L

F

F

I

T

A

A

E

T

K

R

G

G

Q

Q

G

G

A

A

Y
x
Q

L

L

N

N

R

G

Q

Y

K

R

I

L

Q
x
R

V

G

S

S

S

T

K

A

H

R

N

D

I

L

Q

D

E

G

A

T

L

I

V

L

A

A

T

T

G

G

F

F

P

P

Y

F

S

K

I

-

A

A

E

K

G

Q

N

Y

P

A

Q

T

A

T

A

Y

L

I

E

N

L

I

L

V

G

G

T

K

I

L

L

F

K

N

N

E

T

C

R

A

G

D

I

F

R

R

A

T

G

G

S

S

A

A

L

L

D

D

L

L

C

A

Y

Y

V

V

A

A

K

A

G

G

V

R

F

V

D

D

A

G

Y

F

E

E

F

I

D

G

L

L

S

R

P

P

W

W

D

D

I

F

A

A

G

G

A

E

V

L

I

L

A

V

G

R

E

E

A

A

E

G

A

G

H

I

V

V

C

S

G

-

K

-

D

D

F

F

S

T

K

G

S

G

P

H

D

N

-

Y

M

M

Y

L

E

T

K

G

F

N

I

I

M

V

A

A

V

G

T

N

P

P

D

R

I

V

binding magnesium ion: E59 (= E62), D76 (= D78), L78 (≠ I80)
binding : Q126 (≠ Y128), R133 (≠ Q135)

P20456 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Bos taurus (Bovine) (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 18:264/277 of P20456

query
sites
P20456

K

Q

A

A

G

G

E

E

H

V

L

V

L

R

K

E

G

A

F

L

N

K

S

N

V

E

K

M

E

N

I

I

S

M

Y

V

K

K

G

S

K

S

-

P

I

A

D

D

I

L

V

V

T

T

Q

A

F

T

D

D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

T

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

N

N

F

F

V

V

H

H

G

G

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

V

E

V

E

N

G

K

E

K

G

M

I

E

Y

F

G

G

V

I

I

V

Y

Y

N

S

P

C

I

L

L

E

R

D

E

K

M

M

F

Y

I

T

A

G

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

H

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

I

L

I

L

L

G

S

T

N

I

I

L

E

K

R

-

L

-

C

-

L

N

P

T

I

R

H

G

G

I

I

R

R

R

G

A

V

G

G

S

T

A

A

L

L

D

N

L

M

C

C

Y

L

V

V

A

A

K

A

G

G

V

A

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

-

V

-

L

H

D

V

V

C

T

G

G

K

G

D

P

F

F

S

D

K

L

S

-

P

-

D

-

M

M

Y

S

E

R

K

R

F

V

I

I

M

A

A

S

V

S

T

N

P

K

D

T

I

L

S

A

D

E

R

R

L

I

H

A

K

K

E70 (= E62) binding Mg(2+)
D90 (= D78) binding Mg(2+); binding Mg(2+)
I92 (= I80) binding Mg(2+)
D93 (= D81) binding Mg(2+)
D220 (= D207) binding Mg(2+)

2bjiA High resolution structure of myo-inositol monophosphatase, the target of lithium therapy (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 16:262/274 of 2bjiA

query
sites
2bjiA

K

Q

A

A

G

G

E

E

H

V

L

V

L

R

K

E

G

A

F

L

N

K

S

N

V

E

K

M

E

N

I

I

S

M

Y

V

K

K

G

S

K

S

-

P

I

A

D

D

I

L

V

V

T

T

Q

A

F

T

D

D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

T

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

N

N

F

F

V

V

H

H

G

G

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

V

E

V

E

N

G

K

E

K

G

M

I

E

Y

F

G

G

V

I

I

V

Y

Y

N

S

P

C

I

L

L

E

R

D

E

K

M

M

F

Y

I

T

A

G

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

H

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

I

L

I

L

L

G

S

T

N

I

I

L

E

K

R

-

L

-

C

-

L

N

P

T

I

R

H

G

G

I

I

R

R

R

G

A

V

G

G

S

T

A

A

L

L

D

N

L

M

C

C

Y

L

V

V

A

A

K

A

G

G

V

A

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

-

V

-

L

H

D

V

V

C

T

G

G

K

G

D

P

F

F

S

D

K

L

S

-

P

-

D

-

M

M

Y

S

E

R

K

R

F

V

I

I

M

A

A

S

V

S

T

N

P

K

D

T

I

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D88 (= D78), I90 (= I80), D91 (= D81), D218 (= D207)
binding magnesium ion: E68 (= E62), D88 (= D78), D88 (= D78), I90 (= I80), D91 (= D81), D218 (= D207)

2p3nA Thermotoga maritima impase tm1415 (see paper)
38% identity, 90% coverage: 7:236/255 of query aligns to 9:229/256 of 2p3nA

query
sites
2p3nA

K

K

A

L

V

L

T

R

K

K

A

V

G

G

E

H

H

L

L

L

L

M

K

I

G

H

F

W

N

G

S

R

V

V

K

D

E

N

I

V

S

E

Y

K

K

K

-

T

G

G

K

F

I

K

D

D

I

I

V

V

T

T

Q

E

F

I

D

D

V

R

E

E

V

A

E

Q

K

R

I

M

L

I

L

V

N

D

E

E

L

I

S

R

F

K

M

F

P

P

E

D

Y

E

G

N

F

I

V

M

A

A

E
|
E

E

E

T

G

A

I

Q

F

Q

E

K

K

T

G

D

D

K

R

K

L

K

W

V

I

V

-

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T
|
T

T

I

N

N

F

F

V

V

H

H

G

G

F

L

P

P

F

N

C

F

C

S

V

I

S

S

V

L

G

A

V

Y

Y

V

E

E

E

N

G

G

E

E

G

V

I

K

Y

L

G

G

V

V

I

V

Y

H

N

A

P

P

I

A

L

L

R

N

E

E

M

T

F

L

I

Y

A

A

E

E

K

E

G

G

Q

S

G

G

A

A

Y

F

L

F

N

N

R

G

Q

E

K

R

I

I

Q

R

V

V

S

S

S

E

K

N

H

A

N

S

I

L

Q

E

E

E

A

C

L

V

V

G

A

S

T

T

G

G

F

-

P

S

Y

Y

S

V

I

D

A

F

E

T

G

G

N

K

P

F

Q

I

A

E

A

R

L

M

E

E

L

-

G

-

T

-

I

-

L

-

K

K

N

R

T

T

R

R

G

R

I

I

R

R

R

I

A

L

G

G

S

S

A

A

L

L

D

N

L

A

C

A

Y

Y

V

V

A

G

K

A

G

G

V

R

F

V

D

D

A

F

Y

F

Y

V

E

T

F

W

D

R

L

I

S

N

P

P

W

W

D
|
D

I

I

A

A

G

G

A

L

V

I

I

I

A

V

G

K

E

E

A

A

E

G

A

G

H

M

V

V

C

T

G

-

K

-

D

D

F

F

S

S

-

G

K

K

S

E

P

A

D

N

M

A

Y

F

E

S

K

K

active site: D79 (= D78), I81 (= I80), D82 (= D81), T84 (= T83), D201 (= D207)
binding magnesium ion: E65 (= E62), D79 (= D78), I81 (= I80), D82 (= D81)

O33832 Fructose-1,6-bisphosphatase/inositol-1-monophosphatase; FBPase/IMPase; Inositol-1-phosphatase; I-1-Pase; EC 3.1.3.11; EC 3.1.3.25 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
38% identity, 90% coverage: 7:236/255 of query aligns to 9:229/256 of O33832

query
sites
O33832

K

K

A

L

V

L

T

R

K

K

A

V

G

G

E

H

H

L

L

L

L

M

K

I

G

H

F

W

N

G

S

R

V

V

K

D

E

N

I

V

S

E

Y

K

K

K

-

T

G

G

K

F

I

K

D

D

I

I

V

V

T

T

Q

E

F

I

D

D

V

R

E

E

V

A

E

Q

K

R

I

M

L

I

L

V

N

D

E

E

L

I

S

R

F

K

M

F

P

P

E

D

Y

E

G

N

F

I

V

M

A

A

E
|
E

E

E

T

G

A

I

Q

F

Q

E

K

K

T

G

D

D

K

R

K

L

K

W

V

I

V

-

Y

-

I

I

D
|
D

P

P

I
|
I

D

D

G

G

T

T

T

I

N

N

F

F

V

V

H

H

G

G

F

L

P

P

F

N

C

F

C

S

V

I

S

S

V

L

G

A

V

Y

Y

V

E

E

E

N

G

G

E

E

G

V

I

K

Y

L

G

G

V

V

I

V

Y

H

N

A

P

P

I

A

L

L

R

N

E

E

M

T

F

L

I

Y

A

A

E

E

K

E

G

G

Q

S

G

G

A

A

Y

F

L

F

N

N

R

G

Q

E

K

R

I

I

Q

R

V

V

S

S

S

E

K

N

H

A

N

S

I

L

Q

E

E

E

A

C

L

V

V

G

A

S

T

T

G

G

F

-

P

S

Y

Y

S

V

I

D

A

F

E

T

G

G

N

K

P

F

Q

I

A

E

A

R

L

M

E

E

L

-

G

-

T

-

I

-

L

-

K

K

N

R

T

T

R

R

G

R

I

I

R

R

R

I

A

L

G

G

S

S

A

A

L

L

D

N

L

A

C

A

Y

Y

V

V

A

G

K

A

G

G

V

R

F

V

D

D

A

F

Y

F

Y

V

E

T

F

W

D

R

L

I

S

N

P

P

W

W

D

D

I

I

A

A

G

G

A

L

V

I

I

I

A

V

G

K

E

E

A

A

E

G

A

G

H

M

V

V

C

T

G

-

K

-

D

D

F

F

S

S

-

G

K

K

S

E

P

A

D

N

M

A

Y

F

E

S

K

K

E65 (= E62) binding Mg(2+)
D79 (= D78) binding Mg(2+)
I81 (= I80) binding Mg(2+)

1imdA Structural studies of metal binding by inositol monophosphatase: evidence for two-metal ion catalysis (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 14:260/266 of 1imdA

query
sites
1imdA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D
|
D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G

G

S

T

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D43 (= D39), E66 (= E62), D86 (= D78), I88 (= I80), D89 (= D81), T91 (= T83), D216 (= D207)
binding manganese (ii) ion: E66 (= E62), D86 (= D78), D86 (= D78), I88 (= I80), D89 (= D81), D89 (= D81), D216 (= D207)
binding phosphate ion: D86 (= D78), D89 (= D81), G90 (= G82), T91 (= T83)

P29218 Inositol monophosphatase 1; IMP 1; IMPase 1; D-galactose 1-phosphate phosphatase; Inositol-1(or 4)-monophosphatase 1; Lithium-sensitive myo-inositol monophosphatase A1; EC 3.1.3.25; EC 3.1.3.94 from Homo sapiens (Human) (see 5 papers)
35% identity, 95% coverage: 11:251/255 of query aligns to 18:264/277 of P29218

query
sites
P29218

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K
|
K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D

D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y
x
S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G
|
G

S
x
T

A
|
A

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E
|
E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

K36 (= K29) mutation to Q: 50-fold reduction in activity.
E70 (= E62) binding Mg(2+); binding substrate
D90 (= D78) binding Mg(2+); binding Mg(2+)
I92 (= I80) binding Mg(2+)
IDGT 92:95 (= IDGT 80:83) binding substrate
D93 (= D81) binding Mg(2+); mutation to N: Loss of activity.
S165 (≠ Y153) mutation S->A,I: Reduced enzyme activity with myo-inositol 1-phosphate.
GTA 194:196 (≠ GSA 181:183) binding substrate
E213 (= E200) binding substrate; mutation to Q: Strongly reduced affinity for myo-inositol 1-phosphate and strongly reduced enzyme activity with myo-inositol 1-phosphate.
D220 (= D207) binding Mg(2+); binding substrate

2hhmA Structure of inositol monophosphatase, the putative target of lithium therapy (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 14:260/272 of 2hhmA

query
sites
2hhmA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D
|
D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G

G

S

T

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D43 (= D39), E66 (= E62), D86 (= D78), I88 (= I80), D89 (= D81), T91 (= T83), D216 (= D207)
binding sulfate ion: E66 (= E62), D86 (= D78), D89 (= D81), G90 (= G82), T91 (= T83)

1imbA Structural analysis of inositol monophosphatase complexes with substrates (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 14:260/272 of 1imbA

query
sites
1imbA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D
|
D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y
x
S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G

G

S
x
T

A
|
A

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E
|
E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D43 (= D39), E66 (= E62), D86 (= D78), I88 (= I80), D89 (= D81), T91 (= T83), D216 (= D207)
binding l-myo-inositol-1-phosphate: D89 (= D81), G90 (= G82), S161 (≠ Y153), T191 (≠ S182), A192 (= A183), E209 (= E200), D216 (= D207)

1awbA Human myo-inositol monophosphatase in complex with d-inositol-1- phosphate and calcium
35% identity, 95% coverage: 11:251/255 of query aligns to 14:260/272 of 1awbA

query
sites
1awbA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D
|
D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G
x
E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G
|
G

S
x
T

A
|
A

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E
|
E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D43 (= D39), E66 (= E62), D86 (= D78), I88 (= I80), D89 (= D81), T91 (= T83), D216 (= D207)
binding calcium ion: E66 (= E62), D86 (= D78), D86 (= D78), I88 (= I80), D89 (= D81), D216 (= D207)
binding d-myo-inositol-1-phosphate: D86 (= D78), D89 (= D81), G90 (= G82), E158 (≠ G150), G190 (= G181), T191 (≠ S182), A192 (= A183), E209 (= E200)

6giuA Human impase with l-690330 (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 16:262/275 of 6giuA

query
sites
6giuA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D

D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G
x
E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G
|
G

S

T

A
|
A

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E
|
E

F

M

D

G

L

I

S

H

P

C

W
|
W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: E68 (= E62), D88 (= D78), I90 (= I80), D91 (= D81), T93 (= T83), D218 (= D207)
binding [1-(4-oxidanylphenoxy)-1-phosphono-ethyl]phosphonic acid: E68 (= E62), D88 (= D78), D91 (= D81), G92 (= G82), T93 (= T83), E160 (≠ G150), G192 (= G181), A194 (= A183), E211 (= E200), W217 (= W206), D218 (= D207)
binding manganese (ii) ion: E68 (= E62), D88 (= D78), D88 (= D78), I90 (= I80), D91 (= D81), D91 (= D81), D218 (= D207)

6zk0AAA human impase with ebselen (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 15:261/274 of 6zk0AAA

query
sites
6zk0AAA

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D

D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G

G

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E
x
K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L
x
C

N
|
N

R

G

Q
|
Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G

G

S

T

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D

D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

binding N-phenyl-2-selanylbenzamide: K126 (≠ E117), C138 (≠ L129), N139 (= N130), Q141 (= Q132)
binding manganese (ii) ion: E67 (= E62), D87 (= D78), I89 (= I80), D90 (= D81)

4as4A Structure of human inositol monophosphatase 1 (see paper)
35% identity, 95% coverage: 11:251/255 of query aligns to 16:262/274 of 4as4A

query
sites
4as4A

K

Q

A

A

G

G

E

E

H

V

L

V

L

C

K

E

G

A

F

I

N

K

S

N

V

E

K

M

E

N

I

V

S

M

Y

L

K

K

G

S

K

S

-

P

I

V

D

D

I

L

V

V

T

T

Q

A

F

T

D
|
D

V

Q

E

K

V

V

E

E

K

K

I

M

L

L

L

I

N

S

E

S

L

I

S

K

F

E

M

K

M

Y

P

P

E

S

Y

H

G

S

F

F

V

I

A

G

E
|
E

E

E

T

S

-

V

-

A

A

A

Q

G

Q

E

K

K

-

S

-

I

-

L

T

T

D

D

K

N

K

P

K

T

V

W

V

I

Y

-

I

I

D
|
D

P

P

I
|
I

D
|
D

G
|
G

T
|
T

T

T

N

N

F

F

V

V

H

H

G

R

F

F

P

P

F

F

C

V

C

A

V

V

S

S

V

I

G

G

V

F

Y

A

E

V

E

N

G

K

E

K

G

I

I

E

Y

F

G

G

V

V

I

V

Y

Y

N

S

P

C

I

V

L

E

R

G

E

K

M

M

F

Y

I

T

A

A

E

R

K

K

G

G

Q

K

G

G

A

A

Y

F

L

C

N

N

R

G

Q

Q

K

K

I

L

Q

Q

V

V

S

S

S

Q

K

Q

H

E

N

D

I

I

Q

T

E

K

A

S

L

L

V

L

A

V

T

T

G

E

F

L

P

G

Y

S

S

S

I

-

A

-

E

-

G

R

N

T

P

P

Q

E

A

T

A

V

L

R

E

M

L

V

L

L

G

S

T

N

I

M

L

E

K

K

-

L

-

F

-

C

-

I

N

P

T

V

R

H

G

G

I

I

R

R

R

S

A

V

G

G

S

T

A

A

L

V

D

N

L

M

C

C

Y

L

V

V

A

A

K

T

G

G

V

G

F

A

D

D

A

A

Y

Y

E

E

F

M

D

G

L

I

S

H

P

C

W

W

D
|
D

I

V

A

A

A

G

G

A

A

G

V

I

I

I

A

V

G

T

E

E

A

A

E

G

A

G

H

V

V

L

C

-

G

-

K

M

D

D

F

V

S

T

K

G

S

G

P

P

-

F

D

D

M

L

Y

M

E

S

K

R

F

R

I

V

M

I

A

A

V

A

T

N

P

N

D

R

I

I

-

L

S

A

D

E

R

R

L

I

H

A

K

K

active site: D45 (= D39), E68 (= E62), D88 (= D78), I90 (= I80), D91 (= D81), T93 (= T83), D218 (= D207)
binding magnesium ion: E68 (= E62), D88 (= D78), D88 (= D78), I90 (= I80), D91 (= D81), D218 (= D207)
binding phosphate ion: E68 (= E62), D88 (= D78), I90 (= I80), D91 (= D81), G92 (= G82), T93 (= T83)

Query Sequence

>WP_013010454.1 NCBI__GCF_000025725.1:WP_013010454.1
MLSKIEKAVTKAGEHLLKGFNSVKEISYKGKIDIVTQFDVEVEKILLNELSFMMPEYGFV
AEETAQQKTDKKKVVYIDPIDGTTNFVHGFPFCCVSVGVYEEGEGIYGVIYNPILREMFI
AEKGQGAYLNRQKIQVSSKHNIQEALVATGFPYSIAEGNPQAALELLGTILKNTRGIRRA
GSAALDLCYVAKGVFDAYYEFDLSPWDIAAGAVIAGEAEAHVCGKDFSKSPDMYEKFIMA
VTPDISDRLHKMLFM

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory