SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013010726.1 NCBI__GCF_000025725.1:WP_013010726.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1g51B Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution (see paper)
50% identity, 97% coverage: 9:586/596 of query aligns to 2:573/580 of 1g51B

query
sites
1g51B

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active site: R223 (= R228), E225 (= E230), R231 (= R236), Q232 (= Q237), E476 (= E489), G479 (= G492), R531 (= R544)
binding aspartyl-adenosine-5'-monophosphate: E177 (= E182), S199 (= S204), Q201 (= Q206), K204 (= K209), R223 (= R228), Q232 (= Q237), F235 (= F240), Q237 (= Q242), H442 (= H455), E476 (= E489), G478 (= G491), G479 (= G492), G480 (= G493), R483 (= R496), I525 (= I538), A526 (= A539), G528 (= G541), R531 (= R544)
binding adenosine monophosphate: V313 (= V318), Q347 (≠ G359), G348 (= G360), L349 (= L361), A350 (= A362), V389 (≠ G401), A390 (= A402)

1g51A Aspartyl tRNA synthetase from thermus thermophilus at 2.4 a resolution (see paper)
50% identity, 97% coverage: 9:586/596 of query aligns to 2:573/580 of 1g51A

query
sites
1g51A

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active site: R223 (= R228), E225 (= E230), R231 (= R236), Q232 (= Q237), E476 (= E489), G479 (= G492), R531 (= R544)
binding aspartyl-adenosine-5'-monophosphate: E177 (= E182), Q201 (= Q206), K204 (= K209), R223 (= R228), R231 (= R236), Q232 (= Q237), F235 (= F240), Q237 (= Q242), H442 (= H455), H443 (= H456), E476 (= E489), G478 (= G491), G479 (= G492), G480 (= G493), R483 (= R496), I525 (= I538), A526 (= A539), G528 (= G541), R531 (= R544)

1efwA Crystal structure of aspartyl-tRNA synthetase from thermus thermophilus complexed to trnaasp from escherichia coli (see paper)
50% identity, 97% coverage: 9:586/596 of query aligns to 2:573/580 of 1efwA

query
sites
1efwA

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Q
|
Q

V

L

V

V

M

A

S
x
H

P
|
P

E

-

Y

-

N

A

K

S

D

P

V

A

H

Y

A

A

V

T

A

A

D

E

S

R

I

V

R
|
R

N

P

E

E

F

W

V

V

I

V

A

R

V

A

K

K

G

G

K

L

V

V

G

R

M

L

R
|
R

P

P

E
|
E

G

P

T

-

I

-

N
|
N

T

P

K
x
R

L

L

P

A

T

T

G

G

E

R

I

V

E
|
E

V

V

M

E

V

L

D

S

E

A

L

L

K

E

I

V

L

L

N

A

A

E

S

A

Q

K

T
|
T

P

P

P
|
P

F

F

M

P

I

V

D

D

-

A

-

G

-

W

-

R

-

G

-

E

D

E

F

E

T

K

N

E

A

A

N

S

E

E

D
x
E

I

L

R

R

L

L

K

K

Y

Y

R

R

Y

Y

L

L

D

D

L

L

R

R

R

R

R

R

K

R

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M

Q

Q

N

E

N

N

L

L

I

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L

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H

H

N

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V

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G

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L

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V

G

A

G

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L

D

D

K

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Y

Y

F

F

Q

Q

I

I

V

A

K

R

C

C

F

F

R
|
R

D

D

E
|
E

D

D

L

L

R

R

A

A

D

D

R
|
R

Q
|
Q

P

P

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D

F

F

T

T

Q

Q

L

L

D

D

M

L

E

E

M

M

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S

F

F

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V

D

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R

V

E

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D

D

L

V

M

L

E

E

L

L

L

N

E

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G

R

M

L

F

M

I

A

E

H

I

V

F

F

Q

R

N

E

I

A

K

L

G

G

I

V

K

E

L

L

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P

R

L

G

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F

F

P

P

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R

M

L

S

S

Y

Y

D

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N

E

A

A

M

M

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Y

Y

G

G

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S

D

D

A

K

P

P

D

D

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L

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R

F

F

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M

L

F

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L

L

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K

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I

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N

G

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P

L

L

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A

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C

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G

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F

K

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V

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F

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-

V

-

A

-

D

-

G

-

G

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V

-

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A

K

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G

A

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K

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K

N

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L

L

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E

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A

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x
R

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K

A

A

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G

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L

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I

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D

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K

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V

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L

L

L

G

G

L

L

I

K

K

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G

G

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-

Y

F

S

R

F

F

V

L

W

W

V

V

L

V

D

D

F

F

P

P

L

L

L

L

D

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Y

W

D

D

A

E

E

E

E

E

K

E

R

A

Y

W

V

T

A

Y

M

M

H

H

H

H

P

P

F

F

T

T

A

S

P

P

L

H

D

P

E

E

D

D

I

L

P

P

L

L

F

L

D

E

T

K

D

D

P

P

G

G

Q

R

M

V

R

R

A

A

K

L

A

A

Y

Y

D

D

L

L

V

V

L

L

N

N

G

G

S

V

E
|
E

I

V

G

G

G
|
G

G

G

S

S

I

I

R

R

I

I

H

H

R

D

S

P

D

R

V

L

Q

Q

E

A

K

R

M

V

F

F

N

R

A

L

L

L

G

G

F

I

T

G

P

E

E

E

E

E

R

Q

E

R

Y

E

K

K

F

F

G

G

F

F

F

F

I

L

D

E

A

A

L

L

K

E

Y

Y

G

G

T

A

P

P

P

P

H

H

G

G

G

G

I

I

A

A

F

W

G

G

V

L

D

D

R
|
R

I

L

A

L

T

A

I

L

L

M

T

T

G

G

S

S

D

P

S

S

I

I

R

R

D

E

V

V

I

I

A

A

F

F

P

P

K

K

T

N

Q

K

K

E

A

G

T

K

D

D

M

P

M

L

S

T

E

G

A

A

P

P

S

S

K

P

I

V

D

P

E

E

K

E

Q

Q

L

L

K

R

E

E

L

L

active site: R223 (= R228), E225 (= E230), R231 (= R236), Q232 (= Q237), E476 (= E489), G479 (= G492), R531 (= R544)
binding : R27 (= R34), R29 (= R36), D30 (= D37), L31 (≠ H38), G32 (= G39), G33 (= G40), L34 (≠ V41), F36 (= F43), Q47 (= Q54), H51 (≠ S58), P52 (= P59), R64 (= R73), R78 (= R87), E80 (= E89), N82 (= N93), R84 (≠ K95), E91 (= E102), T105 (= T116), P107 (= P118), E125 (≠ D130), R343 (≠ S355)

6sjcB Structure of t. Thermophilus asprs in complex with 5'-o-(n-(l- aspartyl)-sulfamoyl)adenosine (see paper)
50% identity, 97% coverage: 9:586/596 of query aligns to 3:574/581 of 6sjcB

query
sites
6sjcB

R

R

R

R

S

T

H

H

N

Y

C

A

V

G

Q

S

L

L

T

R

A

E

K

T

N

H

I

V

G

G

E

E

E

E

V

V

C

V

L

L

M

E

G

G

W

W

V

V

Q

N

R

R

R

R

R

R

D

D

H

L

G

G

G

G

V

L

I

I

F

F

V

L

D

D

L

L

R

R

D

D

R

R

E

E

G

G

L

L

T

V

Q

Q

V

L

V

V

M

A

S

H

P

P

E

-

Y

-

N

A

K

S

D

P

V

A

H

Y

A

A

V

T

A

A

D

E

S

R

I

V

R

R

N

P

E

E

F

W

V

V

I

V

A

R

V

A

K

K

G

G

K

L

V

V

G

R

M

L

R

R

P

P

E

E

G

P

T

-

I

-

N

N

T

P

K

R

L

L

P

A

T

T

G

G

E

R

I

V

E

E

V

V

M

E

V

L

D

S

E

A

L

L

K

E

I

V

L

L

N

A

A

E

S

A

Q

K

T

T

P

P

P

P

F

F

M

P

I

V

D

D

-

A

-

G

-

W

-

R

-

G

-

E

D

E

F

E

T

K

N

E

A

A

N

S

E

E

D

E

I

L

R

R

L

L

K

K

Y

Y

R

R

Y

Y

L

L

D

D

L

L

R

R

R

R

R

R

K

R

I

M

Q

Q

N

E

N

N

L

L

I

R

T

L

R

R

H

H

N

R

I

V

V

I

R

K

T

A

M

I

R

W

E

D

Y

F

L

L

Y

D

A

R

R

E

D

G

F

F

L

V

D

Q

I

V

E

E

T

T

P

P

F

F

L

L

T

T

K

K

S

S

T

T

P

P

E
|
E

G

G

A

A

R

R

D

D

Y

F

L

L

V

V

P

P

S

Y

R

R

V

H

N

E

P

P

G

G

K

L

C

F

Y

Y

A

A

L

L

P

P

Q

Q

S

S

P

P

Q
|
Q

M

L

F

F

K
|
K

Q

Q

L

M

L

L

M

M

V

V

G

A

G

G

Y

L

D

D

K

R

Y

Y

F

F

Q

Q

I

I

V

A

K

R

C

C

F

F

R
|
R

D

D

E

E

D

D

L

L

R

R

A

A

D

D

R
|
R

Q
|
Q

P

P

E

D

F
|
F

T

T

Q
|
Q

L

L

D

D

M

L

E

E

M

M

S

S

F

F

I

V

D

E

R

V

E

E

D

D

L

V

M

L

E

E

L

L

L

N

E

E

G

R

M

L

F

M

I

A

E

H

I

V

F

F

Q

R

N

E

I

A

K

L

G

G

I

V

K

E

L

L

E

P

R

L

G

P

F

F

P

P

R

R

M

L

S

S

Y

Y

D

E

N

E

A

A

M

M

E

E

K

R

Y

Y

G

G

H

S

D

D

A

K

P

P

D

D

T

L

R

R

F

F

D

G

M

L

F

E

L

L

K

K

T

E

I

V

N

G

E

P

L

L

V

F

A

R

D

Q

C

S

G

G

F

F

K

R

V

V

F

F

R

Q

E

E

V

-

V

-

A

-

D

-

G

-

G

-

V

-

V

A

K

E

A

S

I

V

N

K

A

A

K

L

G

A

A

L

G

P

E

K

K

A

Y

L

S

S

R

R

K

K

N

E

I

V

D

A

E

E

L

L

T

E

E

E

L

V

A

A

V

K

S

R

L

H

G

K

A

A

G

Q

G

G

L

L

A

A

Y

W

I

A

K

R

V

V

N

E

E

E

D

G

S

G

L

F

Q

S

S

G

P

G

I

V

V

A

K

K

F

F

L

L

G

E

D

P

S

V

A

R

D

E

T

A

I

L

V

L

K

Q

A

A

M

T

D

E

G

A

K

R

P

P

G

G

D

D

I

T

I

L

F

L

F

F

G

V

A

A

G

G

K

P

K

R

D

K

I

V

V

A

N

A

L

T

Y

A

M

L

S

G

K

A

V

V

R

R

L

L

K

R

L

A

G

A

K

D

E

L

L

L

G

G

L

L

I

K

K

R

E

E

G

G

D

-

Y

F

S

R

F

F

V

L

W

W

V

V

L

V

D

D

F

F

P

P

L

L

L

L

D

E

Y

W

D

D

A

E

E

E

E

E

K

E

R

A

Y

W

V

T

A

Y

M

M

H

H

H

H

P

P

F

F

T

T

A

S

P

P

L

H

D

P

E

E

D

D

I

L

P

P

L

L

F

L

D

E

T

K

D

D

P

P

G

G

Q

R

M

V

R

R

A

A

K

L

A

A

Y

Y

D

D

L

L

V

V

L

L

N

N

G

G

S

V

E
|
E

I
x
V

G
|
G

G
|
G

G
|
G

S

S

I

I

R
|
R

I

I

H

H

R

D

S

P

D

R

V

L

Q

Q

E

A

K

R

M

V

F

F

N

R

A

L

L

L

G

G

F

I

T

G

P

E

E

E

E

E

R

Q

E

R

Y

E

K

K

F

F

G

G

F

F

F

F

I

L

D

E

A

A

L

L

K

E

Y

Y

G

G

T

A

P

P

P

P

H

H

G

G

G

G

I
|
I

A
|
A

F

W

G
|
G

V

L

D

D

R
|
R

I

L

A

L

T

A

I

L

L

M

T

T

G

G

S

S

D

P

S

S

I

I

R

R

D

E

V

V

I

I

A

A

F

F

P

P

K

K

T

N

Q

K

K

E

A

G

T

K

D

D

M

P

M

L

S

T

E

G

A

A

P

P

S

S

K

P

I

V

D

P

E

E

K

E

Q

Q

L

L

K

R

E

E

L

L

binding 5'-O-(L-alpha-aspartylsulfamoyl)adenosine: E178 (= E182), Q202 (= Q206), K205 (= K209), R224 (= R228), R232 (= R236), Q233 (= Q237), F236 (= F240), Q238 (= Q242), E477 (= E489), V478 (≠ I490), G479 (= G491), G480 (= G492), G481 (= G493), R484 (= R496), I526 (= I538), A527 (= A539), G529 (= G541), R532 (= R544)

4wj3M Crystal structure of the asparagine transamidosome from pseudomonas aeruginosa (see paper)
49% identity, 97% coverage: 10:590/596 of query aligns to 2:583/589 of 4wj3M

query
sites
4wj3M

R

R

S

S

H

H

N

Y

C

C

V

G

Q

Q

L

L

T

N

A

E

K

S

N

L

I

D

G

G

E

Q

E

E

V

V

C

T

L

L

M

C

G

G

W

W

V

V

Q

H

R

R

R

R

R
|
R

D
|
D

H
|
H

G

G

G
|
G

V
|
V

I

I

F
|
F

V

L

D

D

L

V

R

R

D

D

R

R

E

E

G

G

L

L

T

A

Q
|
Q

V

V

V

V

M

F

S

D

P

P

E

D

Y

-

N

R

K

A

D

E

V

T

H

F

A

A

V

K

A

A

D

D

S

R

I

V

R
|
R

N

S

E

E

F

F

V

V

I

V

A

K

V

I

K

T

G

G

K

K

V

V

G
x
R

M

L

R
|
R

P

P

E

E

G

G

T
x
A

I

R

N
|
N

T

P

K

N

L

M

P

A

T

S

G

G

E

S

I

I

E
|
E

V

V

M

L

V

G

D

Y

E

E

L

L

K

E

I

V

L

L

N

N

A

Q

S

A

Q

E

T
|
T

P

P

P

P

F

F

M

P

I

L

D
|
D

D

E

F

Y

T

S

N

D

A
x
V

N

G

E

E

D

E

I

T

R

R

L

L

K

R

Y

Y

R

R

Y

F

L

I

D

D

L

L

R

R

R

R

R

P

K

E

I

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active site: R219 (= R228), E221 (= E230), R227 (= R236), Q228 (= Q237), E482 (= E489), G485 (= G492), R537 (= R544)
binding : R28 (= R36), D29 (= D37), H30 (= H38), G32 (= G40), V33 (= V41), F35 (= F43), Q46 (= Q54), R64 (= R73), R76 (≠ G85), R78 (= R87), A82 (≠ T91), N84 (= N93), E93 (= E102), T107 (= T116), D113 (= D122), V118 (≠ A127)

Q51422 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
49% identity, 97% coverage: 10:590/596 of query aligns to 3:584/591 of Q51422

query
sites
Q51422

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H31 (= H38) mutation to L: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 3.5-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.
G82 (= G90) mutation to K: Enhances enzyme specificity for tRNA(Asp) over tRNA(Asn) by 4.2-fold, by reducing enzyme's ability to misacylate tRNA(Asn) when tested against E.coli tRNA, but shows little effect when tested against P.aeruginosa tRNA.

4wj4A Crystal structure of non-discriminating aspartyl-tRNA synthetase from pseudomonas aeruginosa complexed with tRNA(asn) and aspartic acid (see paper)
49% identity, 97% coverage: 10:590/596 of query aligns to 2:583/585 of 4wj4A

query
sites
4wj4A

R

R

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x
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active site: R219 (= R228), E221 (= E230), R227 (= R236), Q228 (= Q237), E482 (= E489), G485 (= G492), R537 (= R544)
binding aspartic acid: S195 (= S204), Q197 (= Q206), H450 (= H456), R489 (= R496), L531 (≠ I538)
binding : R26 (= R34), R28 (= R36), D29 (= D37), H30 (= H38), G31 (= G39), G32 (= G40), V33 (= V41), F35 (= F43), Q46 (= Q54), R64 (= R73), R76 (≠ G85), P79 (= P88), A82 (≠ T91), N84 (= N93), E93 (= E102), T107 (= T116), P109 (= P118), D113 (= D122), E114 (≠ D123), D117 (≠ N126), E121 (≠ D130), A175 (= A184), E221 (= E230), D222 (= D231), R224 (= R233), A225 (= A234), R227 (= R236), Y346 (≠ L356), A447 (= A453), H449 (= H455), H450 (= H456), R549 (= R556), T557 (= T564), Q558 (= Q565), S559 (≠ K566)

P56459 Aspartate--tRNA(Asp/Asn) ligase; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS; EC 6.1.1.23 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
49% identity, 97% coverage: 10:590/596 of query aligns to 2:575/577 of P56459

query
sites
P56459

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