SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013011212.1 NCBI__GCF_000025725.1:WP_013011212.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

A0R066 Branched-chain-amino-acid aminotransferase; BCAT; EC 2.6.1.42 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see 2 papers)
41% identity, 91% coverage: 26:358/367 of query aligns to 31:367/368 of A0R066

query
sites
A0R066

F

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R101 (= R95) binding pyridoxal 5'-phosphate
Y209 (= Y203) binding pyridoxal 5'-phosphate
K299 (≠ D289) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
T314 (= T304) binding pyridoxal 5'-phosphate

3dtgA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design
41% identity, 91% coverage: 26:358/367 of query aligns to 26:362/363 of 3dtgA

query
sites
3dtgA

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active site: K199 (= K198)
binding O-benzylhydroxylamine: F72 (= F71), Y171 (= Y169), Y204 (= Y203)
binding pyridoxal-5'-phosphate: R96 (= R95), R189 (= R188), K199 (= K198), Y204 (= Y203), E235 (= E233), G238 (≠ S236), L263 (= L258), I266 (= I261), T267 (= T262), T309 (= T304)

3dtfA Structural analysis of mycobacterial branched chain aminotransferase- implications for inhibitor design (see paper)
41% identity, 91% coverage: 26:358/367 of query aligns to 26:362/363 of 3dtfA

query
sites
3dtfA

F

F

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active site: K199 (= K198)
binding sulfate ion: R96 (= R95), I266 (= I261), T267 (= T262), T309 (= T304)

3ht5A Crystal structure of ilve a branched chain amino acid transaminase from mycobacterium tuberculosis (see paper)
39% identity, 89% coverage: 31:358/367 of query aligns to 3:334/335 of 3ht5A

query
sites
3ht5A

S

T

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S
x
G

I
|
I

T
|
T

S

R

L

D

S

S

V

L

M

L

E

Q

L

L

L

A

P

I

S

D

L

A

G

G

Y

F

K

A

V

V

R

E

Q

E

E

R

R

R

V

I

K

D

I

I

T

D

E

E

F

W

L

Q

D

K

G

K

I

A

R

A

S

A

G

G

E

E

I

I

I

T

E

E

A

V

G

F

G

A

F

C

G

G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

V

S

A

E

R

Y

-

I

V

F

R

E

H

N

G

G

A

D

S

I

E

L

F

T

R

V

I

G

A

D

D

G

G

G

Q

I

P

G

G

E

E

N

V

T

T

R

M

K

A

I

L

Y

R

E

D

A

T

Y

L

T

T

A

G

I

I

Q

Q

T

R

G

G

K

T

A

F

A

A

D

D

K

T

L

H

G

G

W

W

L

M

Q

A

K

R

V

L

active site: K171 (= K198)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R68 (= R95), K171 (= K198), Y176 (= Y203), E207 (= E233), G210 (≠ S236), M211 (= M237), N212 (= N238), L235 (= L258), G237 (≠ S260), I238 (= I261), T239 (= T262), T281 (= T304)

5u3fA Structure of mycobacterium tuberculosis ilve, a branched-chain amino acid transaminase, in complex with d-cycloserine derivative (see paper)
38% identity, 89% coverage: 31:358/367 of query aligns to 2:325/326 of 5u3fA

query
sites
5u3fA

S

T

D

D

H

H

M

M

L

V

L

S

I

I

D

D

Y

Y

A

A

K

E

G

G

V

R

-

G

W

W

K

H

D

N

A

A

R

R

I

V

V

I

P

P

Y

Y

G

G

S

P

L

I

S

E

I

L

M

D

P

P

G

S

A

A

V

I

A

V

L

L

H

H

Y

Y

G

A

Q

Q

S

E

I

V

F

F

E

E

G
|
G

A

L

K

K

A

A

F

Y

M

R

H

W

E

A

D

D

G

G

E

S

L

I

Y

V

A

S

F

F

R

R

L

A

E

D

E

A

N

N

A

A

K

A

R
|
R

L

L

N

R

V

S

S

S

A

A

N

R

Q

R

L

L

C

A

M

I

P

P

E

E

I

L

P

P

V

D

D

A

V

V

Q

F

V

I

E

E

G

S

M

L

M

R

K

Q

L

L

L

I

D

A

V

V

E

D

R

K

K

A

W

W

C

V

P

P

-

G

M

A

Q

G

P

G

E

E

S

E

S

A

L

L

Y
|
Y

V

L

R

R

P

P

F

F

M

I

I

F

G

A

T

T

E

E

D

P

A

G

L

L

G

G

M

V

A

R

P

P

S

A

S

T

T

Q

Y

Y

T

R

F

Y

C

L

V

L

L

I

L

A

S

S

P

P

S

A

G

A

A

-

Y

-

S

-

G

-

F

-

K

-

H

-

A

P

V

V

R

S

L

V

L

W

V

V

T

S

E

T

R

E

Y

Y

H

V

R

R

A

A

V

C

S

P

G

G

T

T

G

G

S

A

A

A

K
|
K

C

F

C

G

G

G

N

N

Y
|
Y

G

A

Q

A

A

S

F

L

Q

L

A

A

A

Q

R

A

F

E

S

A

K

A

K

E

Y

N

D

G

A

C

S

D

Q

Q

V

V

L

V

Y

W

L

L

D

D

A

A

-

V

S

E

N

R

Q

R

Y

Y

I

I

E

E

E
|
E

V

M

G

G

S
x
G

M
|
M

N
|
N

H

I

F

F

H

F

I

V

T

L

-

G

-

S

-

G

K

G

D

S

K

A

T

R

V

L

V

V

I

T

P

P

E

E

F

L

T

S

D

G

S

S

I

L

L
|
L

R

P

S
x
G

I
|
I

T
|
T

S

R

L

D

S

S

V

L

M

L

E

Q

L

L

L

A

P

I

S

D

L

A

G

G

Y

F

K

A

V

V

R

E

Q

E

E

R

R

R

V

I

K

D

I

I

T

D

E

E

F

W

L

Q

D

K

G

K

I

A

R

A

S

A

G

G

E

E

I

I

I

T

E

E

A

V

G

F

G

A

F

C

G

G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

V

S

A

E

R

Y

-

I

V

F

R

E

H

N

G

G

A

D

S

I

E

L

F

T

R

V

I

G

A

D

D

G

G

G

Q

I

P

G

G

E

E

N

V

T

T

R

M

K

A

I

L

Y

R

E

D

A

T

Y

L

T

T

A

G

I

I

Q

Q

T

R

G

G

K

T

A

F

A

A

D

D

K

T

L

H

G

G

W

W

L

M

Q

A

K

R

V

L

active site: K162 (= K198)
binding (5-hydroxy-6-methyl-4-{[(3-oxo-2,3-dihydro-1,2-oxazol-4-yl)amino]methyl}pyridin-3-yl)methyl dihydrogen phosphate: G45 (= G73), R67 (= R95), Y110 (= Y137), K162 (= K198), Y167 (= Y203), E198 (= E233), G201 (≠ S236), M202 (= M237), N203 (= N238), L226 (= L258), G228 (≠ S260), I229 (= I261), T230 (= T262), T272 (= T304)

3uyyB Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
34% identity, 92% coverage: 20:358/367 of query aligns to 4:337/337 of 3uyyB

query
sites
3uyyB

D

D

A

W

E

S

S

T

L

L

P

G

F

F

G

S

K

Y

L

I

R

R

S

T

D

D

H

L

M

R

L

Y

L

L

I

A

D

H

Y

W

A

K

K

D

G

G

V

E

W

W

K

D

D

A

A

G

R

T

I

L

V

T

P

E

Y

D

G

N

S

Q

L

I

S

H

I

L

M

A

P

E

G

G

A

S

V

T

A

A

L

L

H

H

Y

Y

G

G

Q

Q

S

Q

I

C

F

F

E

E

G

G

A

L

K

K

A

A

F

Y

M

R

H

C

E

A

D

D

G

G

E

S

L

I

Y

N

A

L

F

F

R

R

L

P

E

D

E

Q

N

N

A

A

K

A

R
|
R

L

M

N

R

V

M

S

S

A

C

N

R

Q

R

L

L

C

L

M

M

P

P

E

E

I

L

P

S

V

D

D

E

V

Q

Q

F

V

I

E

D

G

A

M

C

M

L

K

Q

L

V

D

R

V

A

E

N

R

E

K

H

W

F

C

L

-

P

P

P

M

Y

Q

G

P

T

E

G

S

G

S

S

L

L

Y

Y

V

L

R

R

P

P

F

F

M

V

I

I

G

G

T

V

E

G

D

D

A

N

L

I

G

G

M

V

A

R

P

T

S

A

S

P

T

E

Y

F

T

I

F

F

C

S

V

V

L

F

L

C

S

V

P

P

S

V

G

G

A

P

Y

Y

Y

F

S

K

G

G

F

L

K

T

H

P

A

T

V

N

R

-

L

-

L

F

V

I

T

T

E

S

R

D

Y

Y

H

D

R
|
R

A

A

V

A

S

P

G

H

G

G

T

T

G

G

S

A

A

A

K
|
K

C

V

C

G

G

G

N

N

Y
|
Y

G

A

Q

A

A

S

F

L

Q

L

A

P

A

G

R

Y

F

E

S

A

K

K

Y

R

D

D

A

F

S

A

Q

D

V

V

L

I

Y

Y

L

L

D

D

-

P

A

A

S

T

N

H

Q

T

Y

T

I

I

E

E

E
|
E

V

A

G
|
G

S
x
A

M
x
A

N

N

H

F

F

F

H

A

I

I

T

T

K

Q

D

D

K

G

T

Q

V

K

V

F

I

V

P

T

E

P

F

Q

T

S

D

P

S

S

I

I

L

L

R

P

S
|
S

I
|
I

T
|
T

S

K

L

Y

S

S

V

L

M

L

E

W

L

L

L

A

P

E

S

H

-

R

L

L

G

G

Y

L

K

E

V

V

R

E

Q

E

E

G

R

D

V

I

K

R

I

I

T

D

E

E

F

L

L

-

D

-

G

-

I

-

R

-

S

-

G

G

E

K

I

F

I

S

E

E

A

A

G

G

G

A

F

C

G
|
G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

I

S

G

E

G

Y

-

I

-

F

I

E

Q

N

H

G

G

D

D

I

D

L

F

T

H

V

V

-

F

-

Y

G

S

D

E

G

S

G

E

I

P

G

G

E

P

N

V

T

T

R

R

K

R

I

L

Y

Y

E

D

A

E

Y

L

T

V

A

G

I

I

Q

Q

T

Y

G

G

K

D

A

K

A

E

D

A

K

P

L

E

G

G

W

W

L

I

Q

V

K

K

V

V

active site: K181 (= K198)
binding pyridoxal-5'-phosphate: R79 (= R95), R171 (= R188), K181 (= K198), Y186 (= Y203), E217 (= E233), G219 (= G235), A220 (≠ S236), A221 (≠ M237), S244 (= S260), I245 (= I261), T246 (= T262), G282 (= G303), T283 (= T304)

3uzoA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
34% identity, 92% coverage: 20:358/367 of query aligns to 2:335/335 of 3uzoA

query
sites
3uzoA

D

D

A

W

E

S

S

T

L

L

P

G

F

F

G

S

K

Y

L

I

R

R

S

T

D

D

H

L

M

R

L

Y

L

L

I

A

D

H

Y

W

A

K

K

D

G

G

V

E

W

W

K

D

D

A

A

G

R

T

I

L

V

T

P

E

Y

D

G

N

S

Q

L

I

S

H

I

L

M

A

P

E

G

G

A

S

V

T

A

A

L

L

H

H

Y
|
Y

G

G

Q

Q

S

Q

I

C

F
|
F

E

E

G

G

A

L

K

K

A

A

F

Y

M

R

H

C

E

A

D

D

G

G

E

S

L

I

Y

N

A

L

F

F

R

R

L

P

E

D

E

Q

N

N

A

A

K

A

R
|
R

L

M

N

R

V

M

S

S

A

C

N

R

Q

R

L

L

C

L

M

M

P

P

E

E

I

L

P

S

V

D

D

E

V

Q

Q

F

V

I

E

D

G

A

M

C

M

L

K

Q

L

V

D

R

V

A

E

N

R

E

K

H

W

F

C

L

-

P

P

P

M

Y

Q

G

P

T

E

G

S

G

S

S

L

L

Y

Y

V

L

R
|
R

P

P

F

F

M

V

I

I

G

G

T

V

E

G

D

D

A

N

L

I

G

G

M
x
V

A

R

P

T

S

A

S

P

T

E

Y

F

T

I

F

F

C

S

V

V

L

F

L

C

S

V

P

P

S

V

G

G

A

P

Y
|
Y

Y

F

S

K

G

G

F

L

K

T

H

P

A

T

V

N

R

-

L

-

L

F

V

I

T

T

E

S

R

D

Y

Y

H

D

R

R

A

A

V

A

S

P

G

H

G

G

T

T

G

G

S

A

A

A

K
|
K

C

V

C

G

G

G

N

N

Y
|
Y

G

A

Q

A

A

S

F

L

Q

L

A

P

A

G

R

Y

F

E

S

A

K

K

Y

R

D

D

A

F

S

A

Q

D

V

V

L

I

Y

Y

L

L

D

D

-

P

A

A

S

T

N

H

Q

T

Y

T

I

I

E

E

E
|
E

V

A

G
|
G

S
x
A

M
x
A

N
|
N

H

F

F

F

H

A

I

I

T

T

K

Q

D

D

K

G

T

Q

V

K

V

F

I

V

P

T

E

P

F

Q

T

S

D

P

S

S

I

I

L
|
L

R

P

S
|
S

I
|
I

T
|
T

S

K

L

Y

S

S

V

L

M

L

E

W

L

L

L

A

P

E

S

H

-

R

L

L

G

G

Y

L

K

E

V

V

R

E

Q

E

E

G

R

D

V

I

K

R

I

I

T

D

E

E

F

L

L

-

D

-

G

-

I

-

R

-

S

-

G

G

E

K

I

F

I

S

E

E

A

A

G

G

G

A

F

C

G
|
G

T
|
T

A
|
A

A

A

V

V

V

I

T

T

P

P

V

I

S

G

E

G

Y

-

I

-

F

I

E

Q

N

H

G

G

D

D

I

D

L

F

T

H

V

V

-

F

-

Y

G

S

D

E

G

S

G

E

I

P

G

G

E

P

N

V

T

T

R

R

K

R

I

L

Y

Y

E

D

A

E

Y

L

T

V

A

G

I

I

Q

Q

T

Y

G

G

K

D

A

K

A

E

D

A

K

P

L

E

G

G

W

W

L

I

Q

V

K

K

V

V

active site: K179 (= K198)
binding glutamic acid: Y48 (= Y66), F53 (= F71), R122 (= R139), V134 (≠ M151), Y152 (= Y169), T281 (= T304), A282 (= A305)
binding pyridoxal-5'-phosphate: R77 (= R95), K179 (= K198), Y184 (= Y203), E215 (= E233), G217 (= G235), A218 (≠ S236), A219 (≠ M237), N220 (= N238), L240 (= L258), S242 (= S260), I243 (= I261), T244 (= T262), G280 (= G303), T281 (= T304)

3uzbA Crystal structures of branched-chain aminotransferase from deinococcus radiodurans complexes with alpha-ketoisocaproate and l-glutamate suggest its radio-resistance for catalysis (see paper)
34% identity, 92% coverage: 20:358/367 of query aligns to 2:335/335 of 3uzbA

query
sites
3uzbA

D

D

A

W

E

S

S

T

L

L

P

G

F

F

G

S

K

Y

L

I

R

R

S

T

D

D

H

L

M

R

L

Y

L

L

I

A

D

H

Y

W

A

K

K

D

G

G

V

E

W

W

K

D

D

A

A

G

R

T

I

L

V

T

P

E

Y

D

G

N

S

Q

L

I

S

H

I

L

M

A

P

E

G

G

A

S

V

T

A

A

L

L

H

H

Y

Y

G

G

Q

Q

S

Q

I

C

F

F

E

E

G

G

A

L

K

K

A

A

F

Y

M

R

H

C

E

A

D

D

G

G

E

S

L

I

Y

N

A

L

F

F

R

R

L

P

E

D

E

Q

N

N

A

A

K

A

R
|
R

L

M

N

R

V

M

S

S

A

C

N

R

Q

R

L

L

C

L

M

M

P

P

E

E

I

L

P

S

V

D

D

E

V

Q

Q

F

V

I

E

D

G

A

M

C

M

L

K

Q

L

V

D

R

V

A

E

N

R

E

K

H

W

F

C

L

-

P

P

P

M

Y

Q

G

P

T

E

G

S

G

S

S

L

L

Y
|
Y

V

L

R

R

P

P

F

F

M

V

I

I

G

G

T

V

E

G

D

D

A

N

L

I

G

G

M

V

A

R

P

T

S

A

S

P

T

E

Y

F

T

I

F

F

C

S

V

V

L

F

L

C

S

V

P

P

S

V

G

G

A

P

Y

Y

Y

F

S

K

G

G

F

L

K

T

H

P

A

T

V

N

R

-

L

-

L

F

V

I

T

T

E

S

R

D

Y

Y

H

D

R

R

A

A

V

A

S

P

G

H

G

G

T

T

G

G

S

A

A

A

K
|
K

C

V

C

G

G

G

N

N

Y
|
Y

G

A

Q

A

A

S

F

L

Q

L

A

P

A

G

R

Y

F

E

S

A

K

K

Y

R

D

D

A

F

S

A

Q

D

V

V

L

I

Y

Y

L

L

D

D

-

P

A

A

S

T

N

H

Q

T

Y

T

I

I

E

E

E
|
E

V

A

G
|
G

S
x
A

M
x
A

N
|
N

H

F

F

F

H

A

I

I

T

T

K

Q

D

D

K

G

T

Q

V

K

V

F

I

V

P

T

E

P

F

Q

T

S

D

P

S

S

I

I

L

L

R

P

S
|
S

I
|
I

T
|
T

S

K

L

Y

S

S

V

L

M

L

E

W

L

L

L

A

P

E

S

H

-

R

L

L

G

G

Y

L

K

E

V

V

R

E

Q

E

E

G

R

D

V

I

K

R

I

I

T

D

E

E

F

L

L

-

D

-

G

-

I

-

R

-

S

-

G

G

E

K

I

F

I

S

E

E

A

A

G

G

G

A

F

C

G
|
G

T
|
T

A
|
A

A

A

V

V

V

I

T

T

P

P

V

I

S

G

E

G

Y

-

I

-

F

I

E

Q

N

H

G

G

D

D

I

D

L

F

T

H

V

V

-

F

-

Y

G

S

D

E

G

S

G

E

I

P

G

G

E

P

N

V

T

T

R

R

K

R

I

L

Y

Y

E

D

A

E

Y

L

T

V

A

G

I

I

Q

Q

T

Y

G

G

K

D

A

K

A

E

D

A

K

P

L

E

G

G

W

W

L

I

Q

V

K

K

V

V

active site: K179 (= K198)
binding 2-oxo-4-methylpentanoic acid: Y120 (= Y137), K179 (= K198), T281 (= T304), A282 (= A305)
binding pyridoxal-5'-phosphate: R77 (= R95), K179 (= K198), Y184 (= Y203), E215 (= E233), G217 (= G235), A218 (≠ S236), A219 (≠ M237), N220 (= N238), S242 (= S260), I243 (= I261), T244 (= T262), G280 (= G303), T281 (= T304)

5i5sA X-ray crystal structure at 2.06a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a benzisoxazole compound and an internal aldimine linked plp cofactor. (see paper)
33% identity, 83% coverage: 19:321/367 of query aligns to 19:326/361 of 5i5sA

query
sites
5i5sA

K

K

D

P

A

G

E

E

S

P

L

L

P

V

F
|
F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F
|
F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N
|
N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A
|
A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K198 (= K198)
binding 2-(1,2-benzoxazol-3-yl)ethanoic acid: F26 (= F26), F71 (= F71), K198 (= K198), T236 (≠ S236), T309 (= T304), A310 (= A305)
binding pyridoxal-5'-phosphate: R95 (= R95), R188 (= R188), K198 (= K198), Y203 (= Y203), E233 (= E233), T236 (≠ S236), M237 (= M237), N238 (= N238), L262 (= L258), G264 (≠ S260), V265 (≠ I261), V266 (≠ T262), G308 (= G303), T309 (= T304)

2hdkB Crystal structure of cys315ala-cys318ala mutant of human mitochondrial branched chain aminotransferase (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 13:328/363 of 2hdkB

query
sites
2hdkB

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F

F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F

F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

R

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R

R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M

M

N

N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A
|
A

A

A

V

Q

V

V

T

A

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K200 (= K198)
binding 2-oxo-4-methylpentanoic acid: G310 (= G303), T311 (= T304), A312 (= A305)
binding pyridoxal-5'-phosphate: R97 (= R95), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ S236), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), G310 (= G303), T311 (= T304)

6jifB Crystal structures of branched-chain aminotransferase from pseudomonas sp. Uw4 (see paper)
34% identity, 92% coverage: 22:358/367 of query aligns to 4:334/334 of 6jifB

query
sites
6jifB

E

D

S

K

L

L

P

G

F

F

G

D

K

Y

L

I

R

K

S

T

D

D

H

K

M

R

L

Y

L

L

I

S

D

Y

Y

F

A

R

K

N

G

G

V

E

W

W

K

D

D

K

A

G

R

T

I

L

V

T

P

E

Y

D

G

N

S

V

L

L

S

H

I

I

M

S

P

E

G

G

A

S

V

T

A

A

L

L

H

H

Y

Y

G

G

Q

Q

S

Q

I

C

F

F

E

E

G

G

A

M

K

K

A

A

F

Y

M

R

H

C

E

K

D

D

G

G

E

S

L

I

Y

N

A

L

F

F

R

R

L

P

E

D

E

Q

N

N

A

A

K

L

R
|
R

L

M

N

Q

V

R

S

S

A

C

N

A

Q

R

L

L

C

L

M

M

P

P

E

Q

I

V

P

D

V

T

D

E

V

Q

Q

F

V

I

E

E

G

A

M

C

M

K

K

A

L

V

D

R

V

A

-

N

E

E

R

R

K

F

W

I

C

P

P

P

M

Y

Q

G

P

T

E

G

S

G

S

A

L

L

Y

Y

V

L

R

R

P

P

F

F

M

V

I

I

G

G

T

V

E

G

D

D

A

N

L

I

G

G

M

V

A

R

P

T

S

A

S

P

T

E

Y

F

T

I

F

F

C

S

V

I

L

F

L

C

S

I

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

K

G

G

F

L

K

T

-

P

H

H

A

N

V

F

R

Q

L

I

L

-

V

-

T

-

E

S

R

S

Y

Y

H

D

R
|
R

A

A

V

A

S

P

G

Q

G

G

T

T

G

G

S

A

A

A

K
|
K

C

V

C

G

G

G

N

N

Y
|
Y

G

A

Q

A

A

S

F

L

Q

M

A

P

A

G

R

S

F

K

S

A

K

K

Y

A

D

H

A

F

S

A

Q

D

V

A

L

I

Y

Y

L

L

D

D

A

P

-

M

S

T

N

H

Q

T

Y

K

I

I

E

E

E
|
E

V

V

G

G

S
|
S

M
x
A

N
|
N

H

F

F

F

H

G

I

I

T

T

K

H

D

D

K

N

T

K

V

F

V

V

I

T

P

P

E

N

F

-

T

S

D

P

S

S

I

V

L
|
L

R

P

S
x
G

I
|
I

T
|
T

S

R

L

L

S

S

V

L

M

I

E

E

L

L

L

A

P

K

S

T

-

R

L

L

G

G

Y

M

K

E

V

V

R

V

Q

E

E

G

R

D

V

V

K

F

I

I

T

D

E

K

F

L

L

S

D

D

G

-

I

-

R

-

S

-

G

-

E

-

I

F

I

K

E

E

A

A

G

G

G

A

F

C

G
|
G

T
|
T

A

A

V

V

V

I

T

T

P

P

V

I

S

G

E

G

Y

I

I

D

F

Y

E

N

N

D

G

H

D

L

I

H

L

V

T

F

V

H

G

S

D

E

G

T

G

E

I

V

G

G

E

P

N

V

T

T

R

Q

K

K

I

L

Y

Y

E

K

A

E

Y

L

T

T

A

G

I

V

Q

Q

T

T

G

G

K

D

A

I

A

E

D

A

K

P

L

A

G

G

W

W

L

I

Q

V

K

K

V

V

binding pyridoxal-5'-phosphate: R77 (= R95), R169 (= R188), K179 (= K198), Y184 (= Y203), E215 (= E233), S218 (= S236), A219 (≠ M237), N220 (= N238), L239 (= L258), G241 (≠ S260), I242 (= I261), T243 (= T262), G279 (= G303), T280 (= T304)

5i5yA X-ray crystal structure at 1.81a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with an aryl acetate compound and an internal aldimine linked plp cofactor. (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 13:328/363 of 5i5yA

query
sites
5i5yA

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F
|
F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y
|
Y

G

S

Q

L

S

Q

I

L

F
|
F

E

E

G

G

A

M

K
|
K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y
|
Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M
x
V

A

S

P

Q

S

P

S

T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y
|
Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M

M

N

N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G

G

T
|
T

A
|
A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K200 (= K198)
binding {2-[(5-methyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carbonyl)amino]phenyl}acetic acid: F28 (= F26), Y68 (= Y66), F73 (= F71), K77 (= K75), Y139 (= Y137), V153 (≠ M151), Y171 (= Y169), T238 (≠ S236), T311 (= T304), A312 (= A305)
binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ S236), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), T311 (= T304)

5i5uA X-ray crystal structure at 2.40a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydronaphthalenyl compound and an internal aldimine linked plp cofactor. (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 13:328/363 of 5i5uA

query
sites
5i5uA

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F
|
F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y
|
Y

G

S

Q

L

S

Q

I

L

F
|
F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M
x
V

A

S

P

Q

S

P

S

T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N

N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A
|
A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K200 (= K198)
binding 2-hydroxy-N-[(1R)-1,2,3,4-tetrahydronaphthalen-1-yl]acetamide: F28 (= F26), Y68 (= Y66), F73 (= F71), V153 (≠ M151), K200 (= K198), Y205 (= Y203), T238 (≠ S236), G310 (= G303), T311 (= T304), A312 (= A305)
binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ S236), M239 (= M237), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), G310 (= G303), T311 (= T304)

O15382 Branched-chain-amino-acid aminotransferase, mitochondrial; BCAT(m); Placental protein 18; PP18; EC 2.6.1.42 from Homo sapiens (Human) (see 6 papers)
32% identity, 85% coverage: 11:321/367 of query aligns to 42:357/392 of O15382

query
sites
O15382

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F

F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F

F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R

R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y
|
Y

V

V

R
|
R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S
x
T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R

R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y

Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S

T

M

M

N

N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L

L

R

P

S

G

I

V

T

V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G

G

T

T

A

A

A
x
C

V

Q

V

V

T
x
C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

Y168 (= Y137) binding substrate
R170 (= R139) to Q: in HVLI; reduced catalytic activity; dbSNP:rs749866079
T186 (≠ S155) to R: in dbSNP:rs11548193
K229 (= K198) modified: N6-(pyridoxal phosphate)lysine
E264 (= E233) to K: in HVLI; reduced catalytic activity; dbSNP:rs767653663
C342 (≠ A306) mutation to A: Reduces activity about 6-fold.
C345 (≠ T309) mutation to A: Slight reduction of activity.

1ktaA Human branched chain amino acid aminotransferase : three dimensional structure of the enzyme in its pyridoxamine phosphate form. (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 15:330/365 of 1ktaA

query
sites
1ktaA

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F

F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F
|
F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

R

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N
|
N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A

A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

binding 3-methyl-2-oxobutanoic acid: F75 (= F71), K202 (= K198), Y207 (= Y203)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: R99 (= R95), R192 (= R188), K202 (= K198), Y207 (= Y203), E237 (= E233), T240 (≠ S236), M241 (= M237), N242 (= N238), L266 (= L258), G268 (≠ S260), V269 (≠ I261), V270 (≠ T262), G312 (= G303), T313 (= T304)

1ekfA Crystallographic structure of human branched chain amino acid aminotransferase (mitochondrial) complexed with pyridoxal-5'- phosphate at 1.95 angstroms (orthorhombic form) (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 15:330/365 of 1ekfA

query
sites
1ekfA

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F

F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F

F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

R

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N
|
N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G

G

T
|
T

A

A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K202 (= K198)
binding pyridoxal-5'-phosphate: R99 (= R95), R192 (= R188), K202 (= K198), Y207 (= Y203), E237 (= E233), T240 (≠ S236), M241 (= M237), N242 (= N238), L266 (= L258), G268 (≠ S260), V269 (≠ I261), V270 (≠ T262), T313 (= T304)

5i5tA X-ray crystal structure at 2.31a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a tetrahydroquinoline compound and an internal aldimine linked plp cofactor. (see paper)
32% identity, 83% coverage: 19:321/367 of query aligns to 19:326/361 of 5i5tA

query
sites
5i5tA

K

K

D

P

A

G

E

P

S

P

L

L

P

V

F
|
F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F
|
F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y
|
Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N
|
N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A
|
A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K198 (= K198)
binding (3R)-3-methyl-1,2,3,4-tetrahydroquinoline-8-sulfonamide: F26 (= F26), F71 (= F71), Y169 (= Y169), K198 (= K198), T236 (≠ S236), A310 (= A305)
binding pyridoxal-5'-phosphate: R95 (= R95), R188 (= R188), K198 (= K198), Y203 (= Y203), E233 (= E233), T236 (≠ S236), M237 (= M237), N238 (= N238), L262 (= L258), G264 (≠ S260), V265 (≠ I261), V266 (≠ T262), G308 (= G303), T309 (= T304)

2a1hA X-ray crystal structure of human mitochondrial branched chain aminotransferase (bcatm) complexed with gabapentin (see paper)
32% identity, 85% coverage: 11:321/367 of query aligns to 13:328/363 of 2a1hA

query
sites
2a1hA

S

T

Q

Q

R

K

K

P

C

H

S

K

T

K

I

P

K

G

D

P

A

G

E

E

S

P

L

L

P

V

F

F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y
|
Y

G

S

Q

L

S

Q

I

L

F

F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y

Y

V

V

R
|
R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M
x
V

A

S

P

Q

S

P

S

R

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y

Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

V

V

R

S

L

L

V

A

T

D

E

P

R

A

Y

F

H

I

R
|
R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
|
K

C

L

C

G

G

G

N

N

Y
|
Y

G

G

Q

P

A

T

F

V

Q

L

A

V

A

Q

R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

Q

Q

V

V

L

L

Y

W

L

L

D

Y

A

G

S

P

N

D

Q

H

Y

Q

I

L

E

T

E
|
E

V

V

G

G

S
x
T

M
|
M

N
|
N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

T

E

V

L

V

V

I

T

P

P

E

P

F

L

T

N

D

G

S

V

I

I

L
|
L

R

P

S
x
G

I
x
V

T
x
V

S

R

L

Q

S

S

V

L

M

L

E

D

L

M

L

A

P

Q

S

T

L

W

G

G

-

E

Y

F

K

R

V

V

R

V

Q

E

E

R

R

T

V

I

K

T

I

M

T

K

E

Q

F

L

L

L

D

R

G

A

I

L

R

E

S

E

G

G

E

R

I

V

I

R

E

E

A

V

G

F

G

G

F

S

G
|
G

T
|
T

A
|
A

A

C

V

Q

V

V

T

C

P

P

V

V

S

H

E

R

Y

I

I

L

F

Y

E

K

N

D

G

R

D

N

I

L

active site: K200 (= K198)
binding [1-(aminomethyl)cyclohexyl]acetic acid: Y68 (= Y66), R141 (= R139), V153 (≠ M151), T238 (≠ S236), G310 (= G303), T311 (= T304), A312 (= A305)
binding pyridoxal-5'-phosphate: R97 (= R95), R190 (= R188), K200 (= K198), Y205 (= Y203), E235 (= E233), T238 (≠ S236), M239 (= M237), N240 (= N238), L264 (= L258), G266 (≠ S260), V267 (≠ I261), V268 (≠ T262), T311 (= T304)

5i5vA X-ray crystal structure at 1.94a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a thienopyrimidine compound and an internal aldimine linked plp cofactor. (see paper)
32% identity, 83% coverage: 19:321/367 of query aligns to 18:325/360 of 5i5vA

query
sites
5i5vA

K

K

D

K

A

P

E

G

S

P

L

L

P

V

F
|
F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

V

S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F

F

E

E

G

G

A

M

K
|
K

A

A

F

F

M

K

H

G

E

K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
|
R

L

M

N

L

V

R

S

S

A

A

N

M

Q

R

L

L

C

C

M

L

P

P

E

S

I

F

P

D

V

K

D

L

V

E

Q

L

V

L

E

E

G

C

M

I

M

R

K

R

L

L

L

I

D

E

V

V

E

D

R

K

K

D

W

W

C

V

P

P

M

D

Q

A

P

A

E

G

S

T

S

S

L

L

Y
|
Y

V

V

R

R

P

P

F

V

M

L

I

I

G

G

T

N

E

E

D

P

A

S

L

L

G

G

M

V

A

S

P

Q

S

P

S

T

T

R

Y

A

T

L

F

L

C

F

V

V

L

I

L

L

S

C

P

P

S

V

G

G

A

A

Y
|
Y

Y

F

S

P

G

G

F

S

K

V

H

T

A

P

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V

R

S

L

L

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A

T

D

E

P

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A

Y

F

H

I

R
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R

A

A

V

W

S

V

G

G

T

V

G

G

S

N

A

Y

K
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K

C

L

C

G

G

G

N

N

Y
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Y

G

G

Q

P

A

T

F

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A

V

A

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R

Q

F

E

S

A

K

L

K

K

Y

R

D

G

A

C

S

E

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Q

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V

L

L

Y

W

L

L

D

Y

A

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N

D

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Y

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I

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E

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E
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E

V

V

G

G

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x
T

M
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M

N

N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

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K

L

T

E

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L

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x
G

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x
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x
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S

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M

L

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D

L

M

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A

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Q

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T

L

W

G

G

-

E

Y

F

K

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V

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E

E

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V

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T

I

M

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K

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A

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E

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G
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active site: K197 (= K198)
binding 3,5-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-6-carboxylic acid: F25 (= F26), K74 (= K75), Y136 (= Y137), Y168 (= Y169), A309 (= A305)
binding pyridoxal-5'-phosphate: R94 (= R95), R187 (= R188), K197 (= K198), Y202 (= Y203), E232 (= E233), T235 (≠ S236), M236 (= M237), L261 (= L258), G263 (≠ S260), V264 (≠ I261), V265 (≠ T262), G307 (= G303), T308 (= T304)

5cr5A X-ray crystal structure at 1.61a resolution of human mitochondrial branched chain aminotransferase (bcatm) complexed with a biphenyl pyrrolidine ether compound and an internal aldimine linked plp cofactor. (see paper)
32% identity, 82% coverage: 22:321/367 of query aligns to 19:323/358 of 5cr5A

query
sites
5cr5A

E

K

S

P

L

L

P

V

F
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F

G

G

K

K

L

T

R

F

S

T

D

D

H

H

M

M

L

L

L

M

I

V

D

E

Y

W

A

N

K

D

G

K

V

G

W

W

K

G

D

Q

A

P

R

R

I

I

V

Q

P

P

Y

F

G

Q

S

N

L

L

S

T

I

L

M

H

P

P

G

A

A

S

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S

A

S

L

L

H

H

Y

Y

G

S

Q

L

S

Q

I

L

F
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F

E

E

G

G

A

M

K

K

A

A

F

F

M

K

H

G

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K

D

D

G

Q

E

Q

L

V

Y

R

A

L

F

F

R

R

L

P

E

W

E

L

N

N

A

M

K

D

R
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R

L

M

N

L

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R

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S

A

A

N

M

Q

R

L

L

C

C

M

L

P

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K

D

L

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Q

L

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L

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E

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C

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R

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L

L

I

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E

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V

E

D

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K

D

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W

C

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P

P

M

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A

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A

E

G

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S

S

L

L

Y

Y

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R

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I

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Y

Y

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L

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Y

F

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I

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A

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K

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Q

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E
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E

V
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V

G
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G

S
x
T

M
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M

N
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N

H

I

F

F

-

V

-

Y

-

W

-

T

H

H

I

E

T

D

K

G

D

V

K

L

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E

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L

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V

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x
G

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x
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x
V

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S

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L

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D

L

M

L

A

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G

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E

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L

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I

L

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E

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G

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I

R

E

E

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V

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G

G

F

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G
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G

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A
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A

A

C

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T
x
C

P

P

V

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active site: K195 (= K198)
binding 3-({(3R)-1-[(5-bromothiophen-2-yl)carbonyl]pyrrolidin-3-yl}oxy)-N-methyl-2'-[(methylsulfonyl)amino]biphenyl-4-carboxamide: F23 (= F26), F68 (= F71), Y166 (= Y169), V175 (= V178), Q217 (= Q220), V231 (= V234), G232 (= G235), T233 (≠ S236), M234 (= M237), G305 (= G303), A307 (= A305), C311 (≠ T309)
binding pyridoxal-5'-phosphate: R92 (= R95), K195 (= K198), Y200 (= Y203), E230 (= E233), T233 (≠ S236), N235 (= N238), L259 (= L258), G261 (≠ S260), V262 (≠ I261), V263 (≠ T262), T306 (= T304)

Query Sequence

>WP_013011212.1 NCBI__GCF_000025725.1:WP_013011212.1
MEIKTTLLPESQRKCSTIKDAESLPFGKLRSDHMLLIDYAKGVWKDARIVPYGSLSIMPG
AVALHYGQSIFEGAKAFMHEDGELYAFRLEENAKRLNVSANQLCMPEIPVDVQVEGMMKL
LDVERKWCPMQPESSLYVRPFMIGTEDALGMAPSSTYTFCVLLSPSGAYYSGGFKHAVRL
LVTERYHRAVSGGTGSAKCCGNYGQAFQAARFSKKYDASQVLYLDASNQYIEEVGSMNHF
HITKDKTVVIPEFTDSILRSITSLSVMELLPSLGYKVRQERVKITEFLDGIRSGEIIEAG
GFGTAAVVTPVSEYIFENGDILTVGDGGIGENTRKIYEAYTAIQTGKAADKLGWLQKVPH
FQYDLAR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory