SitesBLAST
Comparing WP_013011526.1 NCBI__GCF_000025725.1:WP_013011526.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5aovA Ternary crystal structure of pyrococcus furiosus glyoxylate hydroxypyruvate reductase in presence of glyoxylate (see paper)
45% identity, 91% coverage: 30:314/314 of query aligns to 34:322/334 of 5aovA
- active site: L100 (= L96), R241 (= R233), D265 (= D257), E270 (= E262), H288 (= H280)
- binding glyoxylic acid: M52 (= M48), L53 (= L49), L53 (= L49), Y74 (= Y70), A75 (= A71), V76 (= V72), G77 (= G73), R241 (= R233), H288 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V72), T104 (= T100), F158 (= F150), G159 (= G151), R160 (= R152), I161 (= I153), S180 (= S172), R181 (= R173), A211 (≠ T203), V212 (≠ A204), P213 (= P205), T218 (= T210), I239 (= I231), A240 (≠ G232), R241 (= R233), H288 (= H280), G290 (= G282)
6biiA Crystal structure of pyrococcus yayanosii glyoxylate hydroxypyruvate reductase in complex with NADP and malonate (re-refinement of 5aow) (see paper)
43% identity, 100% coverage: 2:314/314 of query aligns to 3:321/332 of 6biiA
- active site: L99 (= L96), R240 (= R233), D264 (= D257), E269 (= E262), H287 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V75 (= V72), T103 (= T100), G156 (= G149), F157 (= F150), G158 (= G151), R159 (= R152), I160 (= I153), A179 (≠ S172), R180 (= R173), S181 (≠ T174), K183 (= K176), V211 (≠ A204), P212 (= P205), E216 (= E209), T217 (= T210), V238 (≠ I231), A239 (≠ G232), R240 (= R233), D264 (= D257), H287 (= H280), G289 (= G282)
O58320 Glyoxylate reductase; EC 1.1.1.26 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
43% identity, 100% coverage: 2:314/314 of query aligns to 4:322/334 of O58320
2dbqA Crystal structure of glyoxylate reductase (ph0597) from pyrococcus horikoshii ot3, complexed with NADP (i41) (see paper)
43% identity, 100% coverage: 2:314/314 of query aligns to 4:322/333 of 2dbqA
- active site: L100 (= L96), R241 (= R233), D265 (= D257), E270 (= E262), H288 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V76 (= V72), T104 (= T100), L158 (≠ F150), G159 (= G151), R160 (= R152), I161 (= I153), S180 (= S172), R181 (= R173), T182 (= T174), A211 (≠ T203), V212 (≠ A204), P213 (= P205), T218 (= T210), I239 (= I231), A240 (≠ G232), R241 (= R233), D265 (= D257), H288 (= H280), G290 (= G282)
3bazA Structure of hydroxyphenylpyruvate reductase from coleus blumei in complex with NADP+ (see paper)
38% identity, 83% coverage: 35:295/314 of query aligns to 37:292/311 of 3bazA
- active site: L98 (= L96), R230 (= R233), A251 (= A254), D254 (= D257), E259 (= E262), H277 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V74 (= V72), G149 (= G149), L150 (≠ F150), G151 (= G151), R152 (= R152), I153 (= I153), S172 (= S172), R173 (= R173), S174 (≠ T174), C201 (≠ A204), P202 (= P205), T207 (= T210), I228 (= I231), G229 (= G232), R230 (= R233), D254 (= D257), H277 (= H280), G279 (= G282)
Q65CJ7 Hydroxyphenylpyruvate reductase; HPPR; EC 1.1.1.237 from Plectranthus scutellarioides (Coleus) (Solenostemon scutellarioides) (see paper)
38% identity, 83% coverage: 35:295/314 of query aligns to 39:294/313 of Q65CJ7
Q9UBQ7 Glyoxylate reductase/hydroxypyruvate reductase; EC 1.1.1.79; EC 1.1.1.81 from Homo sapiens (Human) (see paper)
33% identity, 98% coverage: 1:307/314 of query aligns to 7:320/328 of Q9UBQ7
- VG 83:84 (= VG 72:73) binding substrate
- GRI 162:164 (= GRI 151:153) binding NADP(+)
- RQPR 185:188 (≠ RTKK 173:176) binding NADP(+)
- S217 (≠ P205) binding NADP(+)
- I243 (= I231) binding NADP(+)
- R245 (= R233) binding substrate
- D269 (= D257) binding substrate
- HIGS 293:296 (= HIGS 280:283) binding substrate
- G295 (= G282) binding NADP(+)
2gcgA Ternary crystal structure of human glyoxylate reductase/hydroxypyruvate reductase (see paper)
33% identity, 98% coverage: 1:307/314 of query aligns to 3:316/324 of 2gcgA
- active site: L103 (= L96), R241 (= R233), D265 (= D257), E270 (= E262), H289 (= H280)
- binding (2r)-2,3-dihydroxypropanoic acid: L55 (= L49), S78 (≠ A71), V79 (= V72), G80 (= G73), R241 (= R233), H289 (= H280)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V79 (= V72), T107 (= T100), G156 (= G149), G158 (= G151), I160 (= I153), G180 (≠ S172), R181 (= R173), R184 (≠ K176), C212 (≠ A204), S213 (≠ P205), T218 (= T210), I239 (= I231), R241 (= R233), D265 (= D257), H289 (= H280), G291 (= G282)
5v7gA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADPH and oxalate (see paper)
34% identity, 85% coverage: 40:305/314 of query aligns to 40:300/317 of 5v7gA
- active site: L94 (= L96), R228 (= R233), D252 (= D257), E257 (= E262), H275 (= H280)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: V70 (= V72), V98 (≠ T100), F146 (= F148), L148 (≠ F150), G149 (= G151), R150 (= R152), I151 (= I153), T170 (≠ S172), R171 (= R173), V199 (≠ A204), P200 (= P205), S204 (≠ E209), T205 (= T210), V226 (≠ I231), G227 (= G232), R228 (= R233), H275 (= H280), A277 (≠ G282)
- binding oxalate ion: G69 (≠ A71), V70 (= V72), G71 (= G73), R228 (= R233), H275 (= H280)
5v7nA Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and 2-keto-d-gluconic acid (see paper)
34% identity, 85% coverage: 40:305/314 of query aligns to 41:301/319 of 5v7nA
- active site: L95 (= L96), R229 (= R233), D253 (= D257), E258 (= E262), H276 (= H280)
- binding 2-keto-D-gluconic acid: G70 (≠ A71), V71 (= V72), G72 (= G73), R229 (= R233), H276 (= H280), S279 (= S283), R285 (= R289)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V71 (= V72), V99 (≠ T100), L149 (≠ F150), G150 (= G151), R151 (= R152), I152 (= I153), T171 (≠ S172), R172 (= R173), V200 (≠ A204), P201 (= P205), S205 (≠ E209), T206 (= T210), V227 (≠ I231), G228 (= G232), R229 (= R233), H276 (= H280), A278 (≠ G282)
5v6qB Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with NADP and malonate (see paper)
34% identity, 85% coverage: 40:305/314 of query aligns to 42:302/319 of 5v6qB
- active site: L96 (= L96), R230 (= R233), D254 (= D257), E259 (= E262), H277 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V72 (= V72), V100 (≠ T100), F148 (= F148), L150 (≠ F150), G151 (= G151), R152 (= R152), I153 (= I153), T172 (≠ S172), R173 (= R173), V201 (≠ A204), P202 (= P205), S206 (≠ E209), T207 (= T210), V228 (≠ I231), G229 (= G232), R230 (= R233), H277 (= H280), A279 (≠ G282)
5j23A Crystal structure of NADPH-dependent glyoxylate/hydroxypyruvate reductase smc04462 (smghrb) from sinorhizobium meliloti in complex with 2'-phospho-adp-ribose (see paper)
34% identity, 85% coverage: 40:305/314 of query aligns to 40:300/318 of 5j23A
- active site: L94 (= L96), R228 (= R233), D252 (= D257), E257 (= E262), H275 (= H280)
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4r,5r)-3,4,5-trihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: V70 (= V72), L148 (≠ F150), G149 (= G151), R150 (= R152), I151 (= I153), T170 (≠ S172), R171 (= R173), P200 (= P205), S204 (≠ E209), T205 (= T210), R228 (= R233)
4e5pA Thermostable phosphite dehydrogenase a176r variant in complex with nad (see paper)
30% identity, 100% coverage: 2:314/314 of query aligns to 4:326/332 of 4e5pA
- active site: L100 (= L96), R237 (= R233), D261 (= D257), E266 (vs. gap), H292 (= H280)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V72), L100 (= L96), T104 (= T100), G154 (= G151), A155 (≠ R152), I156 (= I153), A175 (≠ S172), R176 (= R173), L208 (≠ A204), P209 (= P205), T214 (= T210), P235 (≠ I231), C236 (≠ G232), R237 (= R233), H292 (= H280)
4e5mA Thermostable phosphite dehydrogenase e175a/a176r in complex with NADP (see paper)
30% identity, 100% coverage: 2:314/314 of query aligns to 4:326/329 of 4e5mA
- active site: L100 (= L96), R237 (= R233), D261 (= D257), E266 (vs. gap), H292 (= H280)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K76 (≠ V72), L100 (= L96), T104 (= T100), G154 (= G151), A155 (≠ R152), I156 (= I153), R176 (= R173), L208 (≠ A204), P209 (= P205), T214 (= T210), P235 (≠ I231), C236 (≠ G232), R237 (= R233), H292 (= H280), G294 (= G282)
4e5kA Thermostable phosphite dehydrogenase in complex with NAD and sulfite (see paper)
30% identity, 100% coverage: 2:314/314 of query aligns to 4:326/329 of 4e5kA
- active site: L100 (= L96), R237 (= R233), D261 (= D257), E266 (vs. gap), H292 (= H280)
- binding nicotinamide-adenine-dinucleotide: K76 (≠ V72), G77 (= G73), L100 (= L96), T104 (= T100), G152 (= G149), G154 (= G151), A155 (≠ R152), I156 (= I153), H174 (vs. gap), E175 (≠ S171), A176 (≠ S172), A207 (≠ T203), L208 (≠ A204), P209 (= P205), P235 (≠ I231), C236 (≠ G232), R237 (= R233), D261 (= D257), H292 (= H280), G294 (= G282)
- binding sulfite ion: M53 (≠ L49), L75 (≠ A71), K76 (≠ V72), G77 (= G73), L100 (= L96), R237 (= R233), H292 (= H280)
6ih6A Phosphite dehydrogenase mutant i151r/p176r/m207a from ralstonia sp. 4506 in complex with non-natural cofactor nicotinamide cytosine dinucleotide
31% identity, 91% coverage: 30:314/314 of query aligns to 34:327/330 of 6ih6A
- binding [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate: T104 (= T100), R151 (≠ F148), G154 (= G151), A155 (≠ R152), V156 (≠ I153), D175 (≠ S172), A207 (≠ T203), V208 (≠ A204), P209 (= P205), T214 (= T210), A235 (≠ I231), C236 (≠ G232), R237 (= R233)
1wwkA Crystal structure of phosphoglycerate dehydrogenase from pyrococcus horikoshii ot3
35% identity, 97% coverage: 1:305/314 of query aligns to 1:303/304 of 1wwkA
- active site: S96 (≠ L96), R230 (= R233), D254 (= D257), E259 (= E262), H278 (= H280)
- binding nicotinamide-adenine-dinucleotide: V100 (≠ T100), G146 (= G149), F147 (= F150), G148 (= G151), R149 (= R152), I150 (= I153), Y168 (= Y170), D169 (≠ S171), P170 (≠ S172), V201 (≠ A204), P202 (= P205), T207 (= T210), T228 (≠ I231), S229 (≠ G232), D254 (= D257), H278 (= H280), G280 (= G282)
6rj5A Crystal structure of phgdh in complex with compound 39 (see paper)
30% identity, 97% coverage: 2:306/314 of query aligns to 3:300/301 of 6rj5A
6plfA Crystal structure of human phgdh complexed with compound 1 (see paper)
30% identity, 97% coverage: 2:306/314 of query aligns to 4:301/305 of 6plfA
6plgA Crystal structure of human phgdh complexed with compound 15 (see paper)
30% identity, 97% coverage: 2:306/314 of query aligns to 3:300/303 of 6plgA
Query Sequence
>WP_013011526.1 NCBI__GCF_000025725.1:WP_013011526.1
MKILITAELPFDIEGYLKGHELDYNKGKQLSAKELAKRAEDADGIISMLSDKIDRELMES
CKNLKVVANYAVGYNNIDVQAASELGITVCNTPDVLTQTTAELGFALMITAARRVSEGDA
FTRNKKFKGWEADLFLGMDMHGKTLGVFGFGRIGQAVAQMASGFNMDIIYSSRTKKHQAE
LLTGASRVSFDDLVRQSDFLIITAPSTPETHHKFTLETFVQMKSSAVLVNIGRGDIIKER
DLVKALENKLIFAAGLDVYEDEPKIDAGLFKLSNAVLAPHIGSGSFATREAMAKMCCDAV
TSVFKDEKPACALN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory