SitesBLAST
Comparing WP_013011589.1 NCBI__GCF_000025725.1:WP_013011589.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 1kl2A
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E53), Y60 (= Y60), G121 (= G121), H122 (= H122), S172 (= S172), F251 (= F251), N341 (= N341)
- binding glycine: S31 (= S31), Y51 (= Y51), Y61 (= Y61), H200 (= H200), R357 (= R357)
- binding pyridoxal-5'-phosphate: S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), T223 (= T223), H225 (= H225), K226 (= K226)
1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 1kl1A
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding glycine: S31 (= S31), H122 (= H122), R357 (= R357)
- binding pyridoxal-5'-phosphate: S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), A171 (≠ H171), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), T223 (= T223), H225 (= H225), K226 (= K226)
1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 1kkpA
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding pyridoxal-5'-phosphate: S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), K226 (= K226)
- binding serine: S31 (= S31), H122 (= H122), R357 (= R357)
1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 1kkjA
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding pyridoxal-5'-phosphate: S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), T223 (= T223), H225 (= H225), K226 (= K226)
2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 2vmyA
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E53), Y60 (= Y60), Y61 (= Y61), L117 (= L117), G121 (= G121), H122 (= H122), L123 (= L123), S172 (= S172), K248 (= K248), F251 (= F251), N341 (= N341), S349 (≠ K349), P350 (= P350), G351 (≠ F351), R357 (= R357)
- binding glycine: S31 (= S31), Y51 (= Y51), Y61 (= Y61), H200 (= H200), K226 (= K226), R357 (= R357)
- binding pyridoxal-5'-phosphate: Y51 (= Y51), S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), T223 (= T223), K226 (= K226), G257 (= G257)
2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
58% identity, 98% coverage: 1:404/412 of query aligns to 1:404/405 of 2vmxA
- active site: Y51 (= Y51), E53 (= E53), D197 (= D197), T223 (= T223), K226 (= K226), R232 (= R232)
- binding allo-threonine: S31 (= S31), H122 (= H122), H200 (= H200), R357 (= R357)
- binding pyridoxal-5'-phosphate: S93 (= S93), G94 (= G94), A95 (≠ S95), H122 (= H122), S172 (= S172), D197 (= D197), A199 (= A199), H200 (= H200), T223 (= T223), K226 (= K226)
3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
55% identity, 99% coverage: 1:407/412 of query aligns to 1:401/404 of 3pgyB
Sites not aligning to the query:
7x5nA Crystal structure of e. Faecium shmt in complex with (+)-shin-1 and plp-ser (see paper)
56% identity, 97% coverage: 7:407/412 of query aligns to 5:407/409 of 7x5nA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E53), Y58 (= Y60), Y59 (= Y61), L115 (= L117), G119 (= G121), H120 (= H122), L121 (= L123), K340 (≠ C340), N341 (= N341), S342 (≠ A342), P350 (= P350), F351 (= F351), R357 (= R357)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S29 (= S31), Y49 (= Y51), E51 (= E53), Y59 (= Y61), S91 (= S93), G92 (= G94), S93 (= S95), H120 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), H198 (= H200), K224 (= K226), R357 (= R357)
7v3dA Complex structure of serine hydroxymethyltransferase from enterococcus faecium and its inhibitor (see paper)
56% identity, 97% coverage: 7:407/412 of query aligns to 5:407/409 of 7v3dA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E53), Y58 (= Y60), L115 (= L117), G119 (= G121), H120 (= H122), L121 (= L123), K340 (≠ C340), S342 (≠ A342), P350 (= P350), F351 (= F351), R357 (= R357)
- binding pyridoxal-5'-phosphate: Y49 (= Y51), S91 (= S93), G92 (= G94), S93 (= S95), H120 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), K224 (= K226), G255 (= G256)
7x5oB Crystal structure of e. Faecium shmt in complex with me-thf and plp- gly (see paper)
56% identity, 97% coverage: 7:407/412 of query aligns to 6:408/412 of 7x5oB
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S30 (= S31), Y50 (= Y51), Y60 (= Y61), S92 (= S93), G93 (= G94), S94 (= S95), H121 (= H122), S171 (= S172), D196 (= D197), A198 (= A199), H199 (= H200), K225 (= K226), R358 (= R357)
- binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E52 (= E53), Y59 (= Y60), L116 (= L117), G119 (= G120), G120 (= G121), H121 (= H122), S171 (= S172), P252 (= P252), N342 (= N341), P351 (= P350)
4wxgA Crystal structure of l-serine hydroxymethyltransferase in complex with a mixture of l-threonine and glycine (see paper)
58% identity, 97% coverage: 7:407/412 of query aligns to 5:407/410 of 4wxgA
- active site: T43 (≠ N45), L45 (= L47), G189 (= G191), A215 (= A217), T218 (≠ V220), R230 (= R232)
- binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-threonine: S29 (= S31), Y49 (= Y51), E51 (= E53), Y59 (= Y61), S91 (= S93), G92 (= G94), S93 (= S95), H120 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), H198 (= H200), T221 (= T223), K224 (= K226), G255 (= G256), R357 (= R357)
4wxfA Crystal structure of l-serine hydroxymethyltransferase in complex with glycine (see paper)
58% identity, 97% coverage: 7:407/412 of query aligns to 5:407/410 of 4wxfA
- active site: T43 (≠ N45), L45 (= L47), G189 (= G191), A215 (= A217), T218 (≠ V220), R230 (= R232)
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S29 (= S31), Y49 (= Y51), Y59 (= Y61), S91 (= S93), G92 (= G94), S93 (= S95), H120 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), H198 (= H200), H223 (= H225), K224 (= K226), G255 (= G256), R357 (= R357)
4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
56% identity, 98% coverage: 4:407/412 of query aligns to 10:412/416 of 4n0wA
- active site: Y57 (= Y51), E59 (= E53), D202 (= D197), T228 (= T223), K231 (= K226), R237 (= R232)
- binding pyridoxal-5'-phosphate: S99 (= S93), G100 (= G94), S101 (= S95), H128 (= H122), D202 (= D197), A204 (= A199), H205 (= H200), K231 (= K226)
4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
56% identity, 98% coverage: 4:407/412 of query aligns to 8:410/414 of 4ot8A
- active site: Y55 (= Y51), E57 (= E53), D200 (= D197), T226 (= T223), K229 (= K226), R235 (= R232)
- binding pyridoxal-5'-phosphate: S97 (= S93), G98 (= G94), S99 (= S95), H126 (= H122), D200 (= D197), A202 (= A199), H203 (= H200), K229 (= K226)
- binding serine: S35 (= S31), E57 (= E53), Y65 (= Y61), H126 (= H122), H203 (= H200), R360 (= R357)
4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
56% identity, 98% coverage: 4:407/412 of query aligns to 3:405/409 of 4otlA
- active site: Y50 (= Y51), E52 (= E53), D195 (= D197), T221 (= T223), K224 (= K226), R230 (= R232)
- binding glycine: S30 (= S31), Y50 (= Y51), Y60 (= Y61), H121 (= H122), K224 (= K226), R355 (= R357)
- binding pyridoxal-5'-phosphate: S92 (= S93), G93 (= G94), S94 (= S95), H121 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), H198 (= H200), K224 (= K226)
8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
58% identity, 93% coverage: 14:398/412 of query aligns to 9:394/402 of 8suiB
8ssyA Room-temperature x-ray structure of thermus thermophilus serine hydroxymethyltransferase (shmt) bound with d-ser in a pseudo- michaelis complex (see paper)
58% identity, 93% coverage: 14:398/412 of query aligns to 9:394/402 of 8ssyA
2dkjA Crystal structure of t.Th.Hb8 serine hydroxymethyltransferase
58% identity, 93% coverage: 14:398/412 of query aligns to 9:394/402 of 2dkjA
- active site: Y46 (= Y51), E48 (= E53), D192 (= D197), T218 (= T223), K221 (= K226), R227 (= R232)
- binding pyridoxal-5'-phosphate: S88 (= S93), G89 (= G94), S90 (= S95), H117 (= H122), S167 (= S172), D192 (= D197), A194 (= A199), H220 (= H225), K221 (= K226)
Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
58% identity, 93% coverage: 14:398/412 of query aligns to 14:399/407 of Q5SI56
- Y51 (= Y51) binding pyridoxal 5'-phosphate
- GS 94:95 (= GS 94:95) binding pyridoxal 5'-phosphate
- S172 (= S172) binding pyridoxal 5'-phosphate
- H200 (= H200) binding pyridoxal 5'-phosphate
- H225 (= H225) binding pyridoxal 5'-phosphate
- K226 (= K226) modified: N6-(pyridoxal phosphate)lysine
- G258 (= G257) binding pyridoxal 5'-phosphate
6ti4A Shmt from streptococcus thermophilus tyr55ser variant in complex with plp/d-serine/lys230 gem diamine complex
57% identity, 97% coverage: 7:407/412 of query aligns to 5:407/410 of 6ti4A
- active site: S49 (≠ Y51), E51 (= E53), D195 (= D197), T221 (= T223), K224 (= K226), R230 (= R232)
- binding magnesium ion: A50 (≠ S52), E51 (= E53), Y58 (= Y60), Y59 (= Y61)
- binding (2~{R})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-3-oxidanyl-propanoic acid: E51 (= E53), Y59 (= Y61), S91 (= S93), G92 (= G94), S93 (= S95), H120 (= H122), S170 (= S172), D195 (= D197), A197 (= A199), H198 (= H200), K224 (= K226), G255 (= G256), R357 (= R357)
Query Sequence
>WP_013011589.1 NCBI__GCF_000025725.1:WP_013011589.1
MEHLSNFDPEVSGLIKQEEQRQIDKIRLIASENYVSHAVLEATGNVLTNKYSEGYPGKRY
YEGQQFIDPIETIAIERAKELFGAEHANVQPYSGSPANMAVYLAFVKPGDTVMGMSLSHG
GHLTHGSPVSITGKYFNIVSYELDRDTGLLNYETIRELAVKSKPKMIIAGHSAYPRQIDF
RKFREIADEVGAVLFVDMAHFAGLVAGGVHPSPVPYADVVSTTTHKTLRGPRGGMLLCKA
EYAAAIDKAVFPGIQGGPHNHTTAGIAVALKEALLPEFKEYAAQVVKNAGKMADCLTSMG
YQLVTGGTENHLLLIDLSNKNITGKQAAKALDKAGIVLNCNAVPYDTRKPFDPSGIRMGL
AAVTSRGFKEAEVEKTAQWINKAIENFENEEVLENISKEVKELCVKFQPPGL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory