SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_013011685.1 NCBI__GCF_000025725.1:WP_013011685.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q56YA5 Serine--glyoxylate aminotransferase; Alanine--glyoxylate aminotransferase; AGT; Asparagine aminotransferase; Serine--pyruvate aminotransferase; EC 2.6.1.45; EC 2.6.1.44; EC 2.6.1.-; EC 2.6.1.51 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
34% identity, 99% coverage: 3:380/382 of query aligns to 9:394/401 of Q56YA5

query
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Q56YA5

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TGT 68:70 (≠ SGT 61:63) binding pyridoxal 5'-phosphate
T148 (= T139) binding pyridoxal 5'-phosphate
QK 200:201 (= QK 190:191) binding pyridoxal 5'-phosphate
K201 (= K191) binding 3-hydroxypyruvate
P251 (= P240) mutation to L: Abolishes aminotransferase activity.
R347 (= R333) binding 3-hydroxypyruvate

6pk3B Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana (see paper)
34% identity, 99% coverage: 3:380/382 of query aligns to 8:393/400 of 6pk3B

query
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binding pyridoxal-5'-phosphate: T67 (≠ S61), G68 (= G62), T69 (= T63), W72 (≠ M66), F94 (= F88), T147 (= T139), D174 (= D165), V176 (≠ I167), Q199 (= Q190), K200 (= K191)

6pk1A Alanine-glyoxylate aminotransferase 1 (agt1) from arabidopsis thaliana in presence of serine (see paper)
34% identity, 99% coverage: 3:380/382 of query aligns to 7:392/399 of 6pk1A

query
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6pk1A

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binding 3-hydroxypyruvic acid: F93 (= F88), R345 (= R333)
binding pyridoxal-5'-phosphate: T66 (≠ S61), G67 (= G62), T68 (= T63), W71 (≠ M66), F93 (= F88), T146 (= T139), D173 (= D165), V175 (≠ I167), K199 (= K191)

1iugA The crystal structure of aspartate aminotransferase which belongs to subgroup iv from thermus thermophilus (see paper)
35% identity, 90% coverage: 5:346/382 of query aligns to 2:334/348 of 1iugA

query
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1iugA

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D

E

I

I

C

A

K

L

T

E

Y

A

G

G

I

L

L

V

V

V

S

E

T

R

I

L

D

D

M

Y

D

P

W

Y

G

G

K

D

A

T

A

P

K

R

P

P

E

E

E

D

I

V

E

-

Q

-

F

-

L

-

K

-

A

A

N

K

P

E

D

G

T

Y

K

A

A

G

V

L

L

L

Q

V

G

H

S

S

E

E

T

T

S

S

T

T

T

G

V

A

Y

L

H

A

P

D

V

L

E

P

E

A

I

L

A

A

K

R

V

A

V

F

R

K

-

E

K

K

N

N

E

P

N

E

T

G

L

L

L

V

I

G

V

A

D

D

G

M

I

V

T

T

S

S

I

L

G

L

V

V

H

G

D

E

T

V

K

A

F

L

D

E

E

A

W

M

G

G

I

V

D

D

I

A

A

A

I

A

T

S

G

G

S

S

Q

Q

K
|
K

A

G

F

L

M

M

L

C

P

P

G

G

L

L

S

G

L

F

I

V

C

A

L

L

S

S

K

P

K

R

A

A

W

L

N

E

F

R

V

L

E

K

K

-

S

-

T

-

I

-

P

P

R

R

-

G

Y

Y

L

L

D

D

L

L

R

A

K

R

E

E

L

L

K

K

S

A

Q

Q

K

K

Q

E

A

G

T

E

T

S

A

A

Y

W

T

T

P

P

A

A

L

I

T

N

L

L

I

V

N

L

G

A

L

V

A

A

V

A

V

V

L

L

E

E

-

E

-

V

-

L

-

P

M

R

F

L

E

E

E

H

G

L

L

A

E

L

N

K

V

A

Y

W

K

Q

R

N

H

A

A

L

V

L

N

Y

G

G

E

V

A

G

T

E

R

E

A

G

A

-

V

-

K

-

A

-

M

-

G

G

F

L

K

R

L

P

L

V

A

P

E

K

T

R

P

F

S

S

N

P

A

A

A

V

T

A

G

A

F

F

Y

Y

L

L

P

P

E

E

D

G

I

V

D

P

G

Y

G

A

K

R

L

V

V

K

K

E

F

A

M

F

R

A

E

Q

K

R

V

-

G

G

I

A

T

V

Y

I

A

A

G

G

Q

Q

D

G

H

P

L

L

K

K

G

G

R

K

I

V

V

F

R

R

I

L

S

S

H

L

L

M

G

G

Y

A

H

Y

D

D

A

R

F

Y

D

E

T

A

I

L

binding phosphate ion: K84 (= K87), K184 (= K191)

3zrqA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
35% identity, 98% coverage: 4:376/382 of query aligns to 2:379/382 of 3zrqA

query
sites
3zrqA

K

K

Y

L

L

L

I

L

A

H

P

V

G

G

P

P

T

T

P

T

V

I

P

K

E

E

K

D

V

V

L

L

L

V

E

A

M

G

T

L

K

E

P

N

V

N

I

V

H

G

H

F

R

T

T

S

S

K

E

E

F

F

S

V

A

E

T

A

F

L

E

A

K

Y

V

S

A

L

N

K

G

G

L

L

K

R

K

Y

V

V

F

M

S

G

T

A

E

S

Q

K

D

N

V

Y

-

Q

-

P

L

L

M

I

L

I

A

P

G

G

S

G

G
|
G

T
|
T

A

S

A

A

M

M

E

E

A

S

A

-

V

V

V

T

N

S

T

L

L

L

N

K

P

P

G

N

D

D

N

K

V

I

L

L

V

V

I

V

N

S

A

N

G

G

K

V

F
|
F

G

G

Q

D

R

R

W

W

R

E

D

Q

I

I

C

F

K

K

T

R

Y

Y

G

P

I

V

L

N

V

V

S

K

T

V

I

L

D

R

M

P

D

S

W

P

G

G

K

D

A

Y

A

V

K

K

P

P

E

G

E

E

I

V

E

E

Q

E

F

E

L

V

K

R

A

K

N

S

P

-

D

E

T

Y

K

K

A

L

V

V

L

A

L

L

Q

T

G

H

S

V

E

E

T
|
T

S

S

T

T

T

G

V

V

Y

R

H

E

P

P

V

V

E

K

E

D

I

V

A

I

K

N

V

K

V

I

R

R

K

K

N

Y

E

V

N

E

T

-

L

L

L

I

I

V

V

V

D
|
D

G

G

I
x
V

T

S

S

S

I

V

G

G

V

A

H

E

D

E

T

V

K

K

F

A

D

E

E

E

W

W

G

N

I

V

D

D

I

V

A

Y

I

L

T

T

G

A

S

S

Q

Q

K
|
K

A

A

F

L

M

G

L

S

P

A

G

G

L

L

S

G

L

L

I

L

C

L

L

L

S

S

K

P

K

K

A

A

W

L

N

S

F

I

V

L

E

D

-

S

K

Q

S

N

T

S

I

I

P

A

R

G

Y

Y

L

L

D

D

L

L

R

R

K

N

E

W

L

L

-

P

-

V

-

M

K

R

S

G

Q

A

K

E

Q

E

A

G

T

K

T

A

A

A

Y
|
Y

-

F

-

A

T
|
T

P

P

A

P

L

V

T

H

L

V

I

I

N

L

G

Q

L

L

A

A

V

E

V

A

L

F

E

R

M

L

F

I

E

E

E

K

E

E

G

G

L

I

E

E

N

N

V

R

Y

I

K

K

R

R

H

H

A

T

V

M

N

V

G

A

E

S

A

A

T

I

R

R

A

A

A

G

V

L

K

E

A

A

M

L

G

G

F

L

K

E

L

I

L

V

A

A

E

R

T

R

P

P

-

E

-

S

-

Y

S

S

N

N

A

T

A

V

T

T

G

G

F

V

Y

I

L

L

-

K

-

V

-

A

-

D

P

P

E

Q

D

K

I

V

D

L

G

A

G

G

K

T

L

V

V

N

K

E

F

-

M

-

R

-

E

-

K

-

V

-

G

G

I

V

T

E

Y

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

K

A

G

F

R

K

I

Y

V

F

R

R

I

I

S

G

H

H

L

M

G

G

Y

W

H

V

D

T

A

P

F

N

D

D

T

A

I

I

T

I

A

A

I

I

S

S

G

V

L

I

E

E

M

R

G

T

L

L

R

R

K

K

F

L

G

G

V

E

D

P

I

I

K

R

L

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

E

E

I

V

L

L

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G62 (= G62), T63 (= T63), F87 (= F88), T137 (= T139), D162 (= D165), V164 (≠ I167), K188 (= K191), Y239 (= Y238), T242 (= T239)

3zrrA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
35% identity, 97% coverage: 6:376/382 of query aligns to 1:376/379 of 3zrrA

query
sites
3zrrA

L

L

I

L

A

H

P
x
V

G

G

P

P

T

T

P

T

V

I

P

K

E

E

K

D

V

V

L

L

L

V

E

A

M

G

T

L

K

E

P

N

V

N

I

V

H

G

H
x
F

R

T

T

S

S

K

E

E

F

F

S

V

A

E

T

A

F

L

E

A

K

Y

V

S

A

L

N

K

G

G

L

L

K

R

K

Y

V

V

F

M

S

G

T

A

E

S

Q

K

D

N

V

Y

-

Q

-

P

L

L

M

I

L

I

A

P

G

G

S
x
G

G
|
G

T
|
T

A

S

A

A

M

M

E

E

A

S

A

-

V

V

V

T

N

S

T

L

L

L

N

K

P

P

G

N

D

D

N

K

V

I

L

L

V

V

I

V

N

S

A

N

G

G

K

V

F
|
F

G

G

Q

D

R

R

W

W

R

E

D

Q

I

I

C

F

K

K

T

R

Y

Y

G

P

I

V

L

N

V

V

S

K

T

V

I

L

D

R

M

P

D

S

W

P

G

G

K

D

A

Y

A

V

K

K

P

P

E

G

E

E

I

V

E

E

Q

E

F

E

L

V

K

R

A

K

N

S

P

-

D

E

T

Y

K

K

A

L

V

V

L

A

L

L

Q

T

G

H

S

V

E

E

T
|
T

S

S

T

T

T

G

V

V

Y

R

H

E

P

P

V

V

E

K

E

D

I

V

A

I

K

N

V

K

V

I

R

R

K

K

N

Y

E

V

N

E

T

-

L

L

L

I

I

V

V

V

D
|
D

G

G

I
x
V

T

S

S

S

I

V

G

G

V

A

H

E

D

E

T

V

K

K

F

A

D

E

E

E

W

W

G

N

I

V

D

D

I

V

A

Y

I

L

T

T

G

A

S

S

Q

Q

K

K

A

A

F

L

M

G

L

S

P

A

G

G

L

L

S

G

L

L

I

L

C

L

L

L

S

S

K

P

K

K

A

A

W

L

N

S

F

I

V

L

E

D

-

S

K

Q

S

N

T

S

I

I

P

A

R

G

Y

Y

L

L

D

D

L

L

R

R

K

N

E

W

L

L

-

P

-

V

-

M

K

R

S

G

Q

A

K

E

Q

E

A

G

T

K

T

A

A

A

Y
|
Y

-

F

-

A

T
|
T

P

P

A

P

L

V

T

H

L

V

I

I

N

L

G

Q

L

L

A

A

V

E

V

A

L

F

E

R

M

L

F

I

E

E

E

K

E

E

G

G

L

I

E

E

N

N

V

R

Y

I

K

K

R

R

H

H

A

T

V

M

N

V

G

A

E

S

A

A

T

I

R

R

A

A

A

G

V

L

K

E

A

A

M

L

G

G

F

L

K

E

L

I

L

V

A

A

E

R

T

R

P

P

-

E

-

S

-

Y

S

S

N

N

A

T

A

V

T

T

G

G

F

V

Y

I

L

L

-

K

-

V

-

A

-

D

P

P

E

Q

D

K

I

V

D

L

G

A

G

G

K

T

L

V

V

N

K

E

F

-

M

-

R

-

E

-

K

-

V

-

G

G

I

V

T

E

Y

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

K

A

G

F

R

K

I

Y

V

F

R
|
R

I

I

S

G

H

H

L

M

G

G

Y

W

H

V

D

T

A

P

F

N

D

D

T

A

I

I

T

I

A

A

I

I

S

S

G

V

L

I

E

E

M

R

G

T

L

L

R

R

K

K

F

L

G

G

V

E

D

P

I

I

K

R

L

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

E

E

I

V

L

L

binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: V4 (≠ P9), F24 (≠ H29), G58 (≠ S61), G59 (= G62), T60 (= T63), F84 (= F88), T134 (= T139), D159 (= D165), V161 (≠ I167), Y236 (= Y238), T239 (= T239), R333 (= R333)

3zrpA Crystal structure and substrate specificity of a thermophilic archaeal serine : pyruvate aminotransferase from sulfolobus solfataricus (see paper)
35% identity, 96% coverage: 10:376/382 of query aligns to 4:375/377 of 3zrpA

query
sites
3zrpA

G

G

P

P

T

T

P

T

V

I

P

K

E

E

K

D

V

V

L

L

L

V

E

A

M

G

T

L

K

E

P

N

V

N

I

V

H

G

H

F

R

T

T

S

S

K

E

E

F

F

S

V

A

E

T

A

F

L

E

A

K

Y

V

S

A

L

N

K

G

G

L

L

K

R

K

Y

V

V

F

M

S

G

T

A

E

S

Q

K

D

N

V

Y

-

Q

-

P

L

L

M

I

L

I

A

P

G

G

S
x
G

G
|
G

T
|
T

A

S

A

A

M

M

E

E

A

S

A

-

V

V

V

T

N

S

T

L

L

L

N

K

P

P

G

N

D

D

N

K

V

I

L

L

V

V

I

V

N

S

A

N

G

G

K

V

F
|
F

G

G

Q

D

R

R

W

W

R

E

D

Q

I

I

C

F

K

K

T

R

Y

Y

G

P

I

V

L

N

V

V

S

K

T

V

I

L

D

R

M

P

D

S

W

P

G

G

K

D

A

Y

A

V

K

K

P

P

E

G

E

E

I

V

E

E

Q

E

F

E

L

V

K

R

A

K

N

S

P

-

D

E

T

Y

K

K

A

L

V

V

L

A

L

L

Q

T

G

H

S

V

E

E

T
|
T

S

S

T

T

T

G

V

V

Y

R

H

E

P

P

V

V

E

K

E

D

I

V

A

I

K

N

V

K

V

I

R

R

K

K

N

Y

E

V

N

E

T

-

L

L

L

I

I

V

V

V

D
|
D

G

G

I
x
V

T

S

S

S

I

V

G

G

V

A

H

E

D

E

T

V

K

K

F

A

D

E

E

E

W

W

G

N

I

V

D

D

I

V

A

Y

I

L

T

T

G

A

S

S

Q

Q

K
|
K

A

A

F

L

M

G

L

S

P

A

G

G

L

L

S

G

L

L

I

L

C

L

L

L

S

S

K

P

K

K

A

A

W

L

N

S

F

I

V

L

E

D

-

S

K

Q

S

N

T

S

I

I

P

A

R

G

Y

Y

L

L

D

D

L

L

R

R

K

N

E

W

L

L

-

P

-

V

-

M

K

R

S

G

Q

A

K

E

Q

E

A

G

T

K

T

A

A

A

Y
|
Y

-

F

-

A

T
|
T

P

P

A

P

L

V

T

H

L

V

I

I

N

L

G

Q

L

L

A

A

V

E

V

A

L

F

E

R

M

L

F

I

E

E

E

K

E

E

G

G

L

I

E

E

N

N

V

R

Y

I

K

K

R

R

H

H

A

T

V

M

N

V

G

A

E

S

A

A

T

I

R

R

A

A

A

G

V

L

K

E

A

A

M

L

G

G

F

L

K

E

L

I

L

V

A

A

E

R

T

R

P

P

-

E

-

S

-

Y

S

S

N

N

A

T

A

V

T

T

G

G

F

V

Y

I

L

L

-

K

-

V

-

A

-

D

P

P

E

Q

D

K

I

V

D

L

G

A

G

G

K

T

L

V

V

N

K

E

F

-

M

-

R

-

E

-

K

-

V

-

G

G

I

V

T

E

Y

F

A

A

G

P

G

G

Q

V

D

-

H

H

L

P

K

A

G

F

R

K

I

Y

V

F

R

R

I

I

S

G

H

H

L

M

G

G

Y

W

H

V

D

T

A

P

F

N

D

D

T

A

I

I

T

I

A

A

I

I

S

S

G

V

L

I

E

E

M

R

G

T

L

L

R

R

K

K

F

L

G

G

V

E

D

P

I

I

K

R

L

F

G

G

S

E

G

G

I

V

A

K

A

A

A

V

E

E

I

V

L

L

binding pyridoxal-5'-phosphate: G57 (≠ S61), G58 (= G62), T59 (= T63), F83 (= F88), T133 (= T139), D158 (= D165), V160 (≠ I167), K184 (= K191), Y235 (= Y238), T238 (= T239)

2dr1A Crystal structure of the ph1308 protein from pyrococcus horikoshii ot3
32% identity, 87% coverage: 6:339/382 of query aligns to 19:355/381 of 2dr1A

query
sites
2dr1A

L

L

I

F

A

T

P

A

G

G

P

P

T

V

P

A

V

C

P

F

E

P

K

E

V

V

L

L

L

E

E

I

M

M

T

K

K

V

P

Q

V

M

I

F

H

S

H

H

R

R

T

S

S

K

E

E

F

Y

S

R

A

K

T

V

F

H

E

M

K

D

V

T

A

V

N

E

G

R

L

L

K

R

K

E

V

F

F

L

S

E

T

V

E

E

Q

K

-

G

D

E

V

V

L

L

M

L

L

V

A

P

G

S

S
|
S

G
|
G

T
|
T

A

G

A

I

M

M

E

E

A

A

A

S

V

I

V

R

N

N

T

G

L

V

N

S

P

K

G

G

D

G

N

K

V

V

L

L

V

V

I

T

N

I

A

I

G

G

K

A

F
|
F

G

G

Q

K

R

R

W

Y

R

K

D

E

I

V

C

V

K

E

T

S

Y

N

G

G

I

R

L

K

V

A

S

V

T

V

I

L

D

E

M

Y

D

E

W

P

G

G

K

K

A

A

A

V

K

K

P

P

E

E

E

D

I

L

E

D

Q

D

F

A

L

L

K

R

A

K

N

N

P

P

D

D

T

V

K

E

A

A

V

V

L

T

L

I

Q

T

G

Y

S

N

E

E

T
|
T

S

S

T

T

T

G

V

V

Y

L

H

N

P

P

V

L

E

P

E

E

I

L

A

A

K

K

V

V

V

A

R

-

K

K

N

E

E

H

N

D

T

K

L

L

L

V

I

F

V

V

D
|
D

G

A

I
x
V

T

S

S

A

I

M

G

G

V

G

H

A

D

D

T

I

K

K

F

F

D

D

E

K

W

W

G

G

I

L

D

D

I

V

A

V

I

F

T

S

G
x
S

S

S

Q

Q

K
|
K

A

A

F

F

M

G

L

V

P

P

G

G

L

L

S

A

L

I

I

G

C

A

L

F

S

S

K

E

K

R

A

F

W

L

N

E

F

I

V

A

E

E

K

K

S

M

T

P

I

E

P

R

R

G

Y

W

Y

Y

L

F

D

D

L

I

R

P

K

L

E

Y

L

V

K

K

S

Y

Q

L

K

K

Q

E

A

K

-

E

T

S

T

T

A

P

Y

S

T

T

P

P

A

P

L

M

T

P

L

Q

I

V

N

F

G

G

L

I

A

N

V

V

V

A

L

L

E

R

M

I

F

I

E

E

E

K

E

M

-

G

G

G

L

K

E

E

N

K

V

W

Y

L

K

E

R

M

H

Y

A

E

V

K

N

R

G

A

E

K

A

M

T

V

R

R

A

E

A

G

V

V

K

R

A

E

M

I

G

G

F

L

K

D

L

I

L

L

A

A

E

E

T

P

-

G

-

H

P

E

S

S

N

P

A

T

A

I

T

T

G

A

F

V

Y

L

L

T

P

P

E

P

D

G

I

I

D

K

G

G

G

D

K

E

L

V

V

Y

K

E

F

A

M

M

R

R

E

K

K

R

V

-

G

G

I

F

T

E

Y

L

A

A

G

K

G

G

Q

Y

D

G

H

S

L

V

K

K

G

E

R

K

I

T

V

F

R

R

I

I

S

G

H

H

L

M

G

G

Y

Y

binding pyridoxal-5'-phosphate: S75 (= S61), G76 (= G62), T77 (= T63), F102 (= F88), T153 (= T139), D178 (= D165), V180 (≠ I167), S201 (≠ G188), K204 (= K191)

3islA Crystal structure of ureidoglycine-glyoxylate aminotransferase (pucg) from bacillus subtilis
30% identity, 98% coverage: 4:378/382 of query aligns to 8:387/387 of 3islA

query
sites
3islA

K

R

Y

T

L

I

I

M

A

T

P

P

G

G

P

P

T

V

P

E

V

V

P

D

E

P

K

R

V

V

L

L

L

R

E

V

M

M

T

S

K

T

P

P

V

V

I

V

H

G

H

Q

R

F

T

D

S

P

E

A

F

F

S

T

A

G

T

I

F

M

E

N

K

E

V

T

A

M

N

E

G

M

L

L

K

R

K

E

V

L

F

F

S

Q

T

T

E

K

Q

N

D

R

-

W

V

A

L

Y

M

P

L

I

A

D

G

G

S
x
T

G
x
S

T
x
R

A

A

A

G

M

I

E

E

A

A

A

V

V

L

V

A

N

S

T

V

L

I

N

E

P

P

G

E

D

D

N

D

V

V

L

L

V

I

I

P

N

I

A

Y

G

G

K

R

F
|
F

G

G

Q

Y

R

L

W

L

R

T

D

E

I

I

C

A

K

E

T

R

Y

Y

G

G

I

A

L

N

V

V

S

H

T

M

I

L

D

E

M

C

D

E

W

W

G

G

K

T

A

V

A

F

K

D

P

P

E

E

E

D

I

I

-

I

E

R

Q

E

F

I

L

K

K

K

A

V

N

K

P

P

D

-

T

-

K

K

A

I

V

V

L

A

L

M

Q

V

G

H

S

G

E

E

T
|
T

S

S

T

T

T

G

V

R

Y

I

H

H

P

P

V

L

E

K

E

A

I

I

A

G

K

E

V

A

V

C

R

R

K

-

N

T

E

E

N

D

T

A

L

L

L

F

I

I

V

V

D
|
D

G

A

I
x
V

T

A

S

T

I

I

G

G

V

G

H

C

D

E

T

V

K

K

F

V

D

D

E

E

W

W

G

K

I

I

D

D

I

A

A

A

I

I

T

G

G

G

S

T

Q
|
Q

K
|
K

A

C

F

L

M

S

L

V

P

P

P

S

G

G

L

M

S

A

L

P

I

I

C

T

L

Y

S

N

K

E

K

R

A

V

W

A

N

D

F

V

V

I

E

A

K

A

S

R

T

N

I

R

P

P

-

I

-

T

R

S

Y

N

Y

Y

L

F

D

D

L

L

R

S

K

Q

-

L

E

E

L

D

K

Y

S

W

Q

S

K

E

Q

R

A

R

T

L

T

N

A

H

Y

H

T

T

P

E

A

A

L

T

T

T

L

M

I

L

N

Y

G

A

L

L

A

R

V

E

V

G

L

V

E

R

M

L

F

V

E

L

E

E

G

G

L

L

E

E

N

T

V

R

Y

F

K

E

R

R

H

H

A

R

V

H

N

H

G

E

E

A

A

A

T

L

R

A

A

A

A

G

V

I

K

K

A

A

M

M

G

G

F

L

K

R

L

L

L

F

A

G

E

D

T

D

P

S

S

C

N

K

-

M

-

P

A

V

T

T

G

C

F

V

Y

E

L

I

P

P

E

G

D

G

I

I

D

D

G

G

G

E

K

S

L

V

V

R

K

D

F

M

M

L

R

L

E

A

K

Q

V

F

G

G

I

I

T

E

Y

I

A

A

G

S

Q

F

D

G

H

P

L

L

K

A

G

G

R

K

I

I

V

W

R

R

I

I

S

G

H

T

L

M

G

G

Y

Y

H

S

D

C

A

R

F

K

D

E

T

N

I

V

T

L

-

F

A

V

I

L

S

A

G

G

L

L

E

E

M

A

G

V

L

L

R

L

K

R

F

H

G

N

V

A

D

G

I

I

K

E

L

A

G

G

S

K

G

A

I

L

A

Q

A

A

E

L

E

D

I

V

L

Y

Q

E

N

N

binding pyridoxal-5'-phosphate: T66 (≠ S61), S67 (≠ G62), R68 (≠ T63), F93 (= F88), T143 (= T139), D168 (= D165), V170 (≠ I167), Q193 (= Q190), K194 (= K191)

1vjoA Crystal structure of alanine--glyoxylate aminotransferase (alr1004) from nostoc sp. At 1.70 a resolution (see paper)
30% identity, 93% coverage: 3:357/382 of query aligns to 18:377/377 of 1vjoA

query
sites
1vjoA

K

S

K

R

Y

L

L

L

I

L

A

G

P

P

G

G

P

P

T

S

P

N

V

A

P

H

E

P

K

S

V

V

L

L

L

Q

E

A

M

M

T

N

K

V

P

S

V

P

I

V

H

G

H

H

R

L

T

D

S

P

E

A

F

F

S

L

A

A

T

L

F

M

E

D

K

E

V

I

A

Q

N

S

G

L

L

L

K

R

K

Y

V

V

F

W

S

Q

T

T

E

E

Q

N

D

P

V

L

-

T

L

I

M

A

L

V

A

S

G

G

S
x
T

G
|
G

T
|
T

A

A

M

M

E

E

A

A

A

T

V

I

V

A

N

N

T

A

L

V

N

E

P

P

G

G

D

D

N

V

V

V

L

L

V

I

I

G

N

V

A

A

G

G

K

Y

F
|
F

G

G

Q

N

R

R

W

L

R

V

D

D

I

M

C

A

K

G

T

R

Y

Y

G

G

I

A

L

D

V

V

S

R

T

T

I

I

D

S

M

K

D

P

W

W

G

G

K

E

A

V

A

F

K

S

P

L

E

E

I

L

E

R

Q

T

F

A

L

L

K

E

A

T

N

H

P

R

D

P

T

A

K

I

A

L

V

A

L

L

L

V

Q

H

G

-

S

A

E

E

T
|
T

S

S

T

T

T

G

V

A

Y

R

H

Q

P

P

V

L

E

E

E

G

I

V

A

G

K

E

V

L

V

C

R

R

K

E

N

F

E

-

N

G

T

T

L

L

I

L

V

V

D
|
D

G

T

I
x
V

T

T

S

S

I

L

G

G

V

G

H

V

D

P

T

I

K

F

F

L

D

D

E

A

W

W

G

G

I

V

D

D

I

L

A

A

I

Y

T

S

G

C

S

S

Q

Q

K
|
K

A

G

F

L

M

G

L

C

P

S

P

P

G

G

L

A

S

S

L

P

I

F

C

T

L

M

S

S

K

S

K

R

A

A

W

I

N

E

F

K

V

L

E

Q

-

R

K

R

S

T

T

K

I

V

P

A

R

N

Y

W

Y

Y

L

L

D

D

L

M

R

N

-

L

-

G

K

K

E

Y

L

W

K

G

S

S

Q

E

K

R

Q

-

A

-

T

V

T

Y

A

H

Y

H

T

T

P

A

A

P

L

I

T

N

L

L

I

Y

N

Y

G

A

L

L

A

R

V

E

V

A

L

L

E

R

M

L

F

I

E

A

E

Q

E

E

G

G

L

L

E

A

N

N

V

C

Y

W

K

Q

R

R

H

H

A

Q

V

K

N

N

G

V

E

E

A

Y

T

L

R

W

A

E

A

R

V

L

K

E

A

D

M

I

G

G

F

L

K

S

L

L

L

H

A

V

E

E

T

K

P

E

S

Y

N

R

-

L

-

P

A

T

A

L

T

T

G

T

F

V

Y

C

L

I

P

P

E

D

D

G

I

V

D

D

G

G

G

K

K

A

L

V

V

A

K

R

F

R

M

L

R

L

E

N

K

E

V

H

G

N

I

I

T

E

Y

V

A

G

G

G

Q

L

D

G

H

E

L

L

K

A

G

G

R

K

I

V

V

W

R

R

I

V

S

G

H

L

L

M

G

G

Y

F

H

N

D

S

A

R

F

K

D

E

T

S

I

V

T

D

A

Q

-

L

I

I

S

P

G

A

L

L

E

E

M

Q

G

V

L

L

R

R

binding pyridoxal-5'-phosphate: T77 (≠ S61), G78 (= G62), T79 (= T63), F104 (= F88), T154 (= T139), D179 (= D165), V181 (≠ I167), K205 (= K191)

O32148 (S)-ureidoglycine--glyoxylate transaminase; UGXT; (S)-ureidoglycine--glyoxylate aminotransferase; Purine catabolism protein PucG; EC 2.6.1.112 from Bacillus subtilis (strain 168) (see paper)
28% identity, 98% coverage: 4:378/382 of query aligns to 12:409/416 of O32148

query
sites
O32148

K

R

Y

T

L

I

I

M

A

T

P

P

G

G

P

P

T

V

P

E

V

V

P

D

E

P

K

R

V

V

L

L

L

R

E

V

M

M

T

S

K

T

P

P

V

V

I

V

H

G

H
x
Q

R

F

T

D

S

P

E

A

F

F

S

T

A

G

T

I

F

M

E

N

K

E

V

T

A

M

N

E

G

M

L

L

K

R

K

E

V

L

F

F

S

Q

T

T

E

K

Q

N

D

R

-

W

V

A

L

Y

M

P

L

I

A

D

G

G

S

T

G

S

T

R

A

A

A

G

M

I

E

E

A

A

A

V

V

L

V

A

N

S

T

V

L

I

N

E

P

P

G

E

D

D

N

D

V

V

L

L

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I

I

P

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I

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Y

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R

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F

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Y

R

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W

L

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T

D

E

I

I

C

A

K

E

T

R

Y

Y

G

G

I

A

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I

L

D

E

M

C

D

E

W

W

G

G

K

T

A

V

A

F

K

D

P

P

E

E

E

D

-

I

I

I

E

R

Q

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F

I

L

K

K

K

A

V

N

K

P

P

D

-

T

-

K

K

A

I

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V

L

A

L

M

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G

H

S

G

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E

T

T

S

S

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T

T

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Y

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P

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L

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K

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A

I

I

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K

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A

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-

N

T

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E

N

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A

L

L

L

F

I

I

V

V

D

D

G

A

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I

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C

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F

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D

D

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E

W

W

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K

I

I

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D

I

A

A

A

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Q

K
|
K

A

C

F

L

M

S

L

V

P

P

P

S

G

G

L

M

S

A

L

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I

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A

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I

-

A

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A

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D

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A

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L

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S

-

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N

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I

I

P

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Y

N

Y

Y

L

F

D

D

L

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-

L

E

E

L

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K

Y

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W

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S

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A

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L

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x
N

A

H

Y

H

T

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E

A

A

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T

T

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M

I

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Y

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G

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T

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R

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H

A

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H

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A

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A

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A

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K

A

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L

L

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A

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-

M

-

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A

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P

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D

G

I

I

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D

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M

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F

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Y

I

A

A

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S

Q

F

D

G

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P

L

L

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A

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G

R

K

I

I

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W

R

R

I

I

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H

T

L

M

G

G

Y

Y

H

S

D

C

A

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F

K

D

E

T

N

I

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T

L

-

F

A

V

I

L

S

A

G

G

L

L

E

E

M

A

G

V

L

L

R

L

K

R

F

H

G

N

V

A

D

G

I

I

K

E

L

A

G

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K

G

A

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A

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A

A

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L

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D

I

V

L

Y

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E

N

N

Q37 (≠ H29) mutation to H: 5-fold decrease in transamination activity.
K198 (= K191) modified: N6-(pyridoxal phosphate)lysine
N264 (≠ T236) mutation to S: 9-fold decrease in transamination activity.; mutation to Y: Loss of transamination activity.

Q988B8 Pyridoxamine--pyruvate transaminase; Pyridoxamine-pyruvate aminotransferase; EC 2.6.1.30 from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099) (Mesorhizobium loti (strain MAFF 303099)) (see 2 papers)
27% identity, 96% coverage: 10:377/382 of query aligns to 17:390/393 of Q988B8

query
sites
Q988B8

G

G

P

P

T

V

P

N

V

A

P

Y

E

P

K

E

V

V

L

L

L

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E

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G

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Y

H

D

R

Y

T

D

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P

E

A

F

F

S

Q

A

L

T

L

F

Y

E

E

K

K

V

V

A

V

N

D

G

K

L

A

K

Q

K

K

V

A

F

M

S

R

T

L

E

S

Q

N

D

K

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P

L

V

M

I

L

L

A

H

G

G

S
x
E

G

P

T

V

A

L

A

G

M

L

E

E

A

A

V

A

N

S

T

L

L

I

N

S

P

P

G

D

D

D

N

V

V

V

L

L

V

N

I

L

N

A

A

S

G

G

K

V

F
x
Y

G

G

Q

K

R

G

W

F

R

G

D

Y

I

W

C

A

K

K

T

R

Y

Y

G

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H

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L

S

L

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E

I

I

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E

M

V

D

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W

Y

G

N

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E

A

A

A

I

K

D

P

P

E

Q

E

A

I

V

E

A

Q

D

F

M

L

L

K

K

A

A

N

H

P

P

D

E

T

I

K

T

A

V

V

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L

S

L

V

Q

C

G

H

S

H

E

D

T
|
T

S

P

T

S

T

G

V

T

Y

I

H

N

P

P

V

I

E

D

E

A

I

I

A

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A

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L

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A

N

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-

N

G

T

A

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Y

L

L

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I

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D

D

G

A

I

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S

S

S

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G

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M

D

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T

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E

E

D

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A

D

D

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I

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|
K

A
x
C

F

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G

L

A

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P

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G

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T

L

M

I

M

C

G

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S

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A

A

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A

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K

A

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Y

A

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M

-

L

-

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D

I

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E

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A

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P

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I

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N

G

G

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L

A

D

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A

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E

D

M

L

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Y

E

L

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N

E

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E

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N

A

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A

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H

A

A

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A

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A

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A

A

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A

A

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L

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L

W

A

A

E

A

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S

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D

S

S

N

I

A

A

A

S

-

P

-

T

T

T

G

A

F

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Y

R

L

T

P

P

E

D

D

G

I

V

D

D

G

E

G

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K

A

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F

A

M

A

R

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E

A

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R

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Y

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I

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x
R

D

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E

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K

L

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I

L

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|
R

I

I

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G

H

H

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G

G

-

P

Y

T

H

A

D

Q

A

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F

I

D

Y

T

A

I

I

T

A

A

A

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L

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G

A

L

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G

G

A

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A

F

A

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A

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G

G

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I

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E

A

A

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L

E

A

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L

I

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D

E68 (≠ S61) binding pyridoxal 5'-phosphate; mutation E->A,G: Low but detectable pyridoxamine--pyruvate transaminase activity.
Y95 (≠ F88) binding pyridoxal 5'-phosphate
T146 (= T139) binding pyridoxal 5'-phosphate
K197 (= K191) modified: N6-(pyridoxal phosphate)lysine; mutation to L: Loss of function.
C198 (≠ A192) mutation to A: No effect on enzyme activity.
R336 (≠ Q324) mutation to A: Strongly decreased affinity for pyruvate.
R345 (= R333) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2z9xA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxyl-l-alanine (see paper)
27% identity, 96% coverage: 10:377/382 of query aligns to 16:389/392 of 2z9xA

query
sites
2z9xA

G

G

P

P

T

V

P

N

V

A

P

Y

E

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E

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V

L

L

L

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M

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V

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L

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Y

H

D

R

Y

T

D

S

P

E

A

F

F

S

Q

A

L

T

L

F

Y

E

E

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K

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V

A

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D

G

K

L

A

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Q

K

K

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A

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M

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R

T

L

E

S

Q

N

D

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L

V

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I

L

L

A

H

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S
x
E

G

P

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V

A

L

A

G

M

L

E

E

A

A

V

A

N

S

T

L

L

I

N

S

P

P

G

D

D

D

N

V

V

V

L

L

V

N

I

L

N

A

A

S

G

G

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V

F
x
Y

G

G

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R

G

W

F

R

G

D

Y

I

W

C

A

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K

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R

Y

Y

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S

I

P

L

H

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L

S

L

T

E

I

I

D

E

M

V

D

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W

Y

G

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K

E

A

A

A

I

K

D

P

P

E

Q

E

A

I

V

E

A

Q

D

F

M

L

L

K

K

A

A

N

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P

P

D

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T

I

K

T

A

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V

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L

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C

G

H

S

H

E

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T
|
T

S

P

T

S

T

G

V

T

Y

I

H

N

P

P

V

I

E

D

E

A

I

I

A

G

K

A

V

L

V

V

R

S

K

A

N

H

E

-

N

G

T

A

L

Y

L

L

I

I

V

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D
|
D

G

A

I
x
V

T

S

S

S

I

F

G

G

V

G

H

M

D

K

T

T

K

H

F

P

D

E

E

D

W

C

G

K

I

A

D

D

I

I

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I

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T

T

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G

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K
|
K

A

C

F

L

M

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L

A

P

P

G

G

L

L

S

T

L

M

I

M

C

G

L

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S

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E

K

R

A

A

W

W

N

A

F

K

V

M

E

K

K

A

S

N

T

P

I

L

-

A

P

P

R

R

Y

A

Y

S

L

M

-

L

-

S

D

I

L

V

R

D

K

W

E

E

L

N

K

A

S

W

Q

S

K

R

Q

D

A

K

T

P

T

F

A

P

Y

F

T

T

P

P

A

S

L

V

T

S

L

E

I

I

N

N

G

G

L

L

A

D

V

V

V

A

L

L

E

D

M

L

F

Y

E

L

E

N

E

E

G

G

L

P

E

E

N

A

V

V

Y

W

K

A

R

R

H

H

A

A

V

L

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T

G

A

E

K

A

A

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M

R

R

A

A

A

G

V

V

K

T

A

A

M

M

G

G

F

L

K

S

L

V

L

W

A

A

E

A

T

S

P

D

S

S

N

I

A

A

A

S

-

P

-

T

T

T

G

A

F

V

Y

R

L

T

P

P

E

D

D

G

I

V

D

D

G

E

G

K

K

A

L

L

V

R

K

Q

F

A

M

A

R

R

E

A

K

R

V

Y

G

G

I

V

T

V

Y

F

A

S

G

S

G

G

Q

R

D

G

H

E

L

T

K

L

G

G

R

K

I

L

V

T

R
|
R

I

I

S

G

H

H

L

M

G

G

-

P

Y

T

H

A

D

Q

A

P

F

I

D

Y

T

A

I

I

T

A

A

A

I

L

S

T

G

A

L

L

E

G

M

G

G

A

L

M

R

N

K

A

F

A

G

G

V

R

D

K

I

L

K

A

L

I

G

G

S

K

G

G

I

I

A

E

A

A

E

L

E

A

I

V

L

I

Q

D

binding alanine: Y94 (≠ F88), R344 (= R333)
binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S61), Y94 (≠ F88), T145 (= T139), D170 (= D165), V172 (≠ I167), K196 (= K191)

2z9wA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxal (see paper)
27% identity, 96% coverage: 10:377/382 of query aligns to 16:389/392 of 2z9wA

query
sites
2z9wA

G

G

P

P

T

V

P

N

V

A

P

Y

E

P

K

E

V

V

L

L

L

R

E

G

M

L

T

G

K

R

P

T

V

V

I

L

H

Y

H

D

R

Y

T

D

S

P

E

A

F

F

S

Q

A

L

T

L

F

Y

E

E

K

K

V

V

A

V

N

D

G

K

L

A

K

Q

K

K

V

A

F

M

S

R

T

L

E

S

Q

N

D

K

V

P

L

V

M

I

L

L

A

H

G

G

S
x
E

G
x
P

T

V

A

L

A

G

M

L

E

E

A

A

V

A

N

S

T

L

L

I

N

S

P

P

G

D

D

D

N

V

V

V

L

L

V

N

I

L

N

A

A

S

G

G

K

V

F
x
Y

G

G

Q

K

R

G

W

F

R

G

D

Y

I

W

C

A

K

K

T

R

Y

Y

G

S

I

P

L

H

V

L

S

L

T

E

I

I

D

E

M

V

D

P

W

Y

G

N

K

E

A

A

A

I

K

D

P

P

E

Q

E

A

I

V

E

A

Q

D

F

M

L

L

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K

A

A

N

H

P

P

D

E

T

I

K

T

A

V

V

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S

L

V

Q

C

G

H

S

H

E

D

T
|
T

S

P

T

S

T

G

V

T

Y

I

H

N

P

P

V

I

E

D

E

A

I

I

A

G

K

A

V

L

V

V

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S

K

A

N

H

E

-

N

G

T

A

L

Y

L

L

I

I

V

V

D
|
D

G

A

I
x
V

T
x
S

S

S

I

F

G

G

V

G

H

M

D

K

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binding 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde: E67 (≠ S61), P68 (≠ G62), Y94 (≠ F88), T145 (= T139), D170 (= D165), V172 (≠ I167), S173 (≠ T168), K196 (= K191)

2z9vA Crystal structure of pyridoxamine-pyruvate aminotransferase complexed with pyridoxamine (see paper)
27% identity, 96% coverage: 10:377/382 of query aligns to 16:389/392 of 2z9vA

query
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binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: E67 (≠ S61), V69 (≠ T63), Y94 (≠ F88), T145 (= T139), D170 (= D165), V172 (≠ I167)

P21549 Alanine--glyoxylate aminotransferase; AGT; Serine--pyruvate aminotransferase; SPT; EC 2.6.1.44; EC 2.6.1.51 from Homo sapiens (Human) (see 24 papers)
28% identity, 91% coverage: 4:350/382 of query aligns to 23:377/392 of P21549

query
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P21549

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R36 (≠ K17) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177157
G41 (≠ M22) to E: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177168; to R: in HP1; associated in cis with L-11 and M-340; results in loss of protein stability; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; partial mitochondrial mistargeting; intraperoxisomal protein aggregation seen; dbSNP:rs121908523; to V: in HP1; reduced alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177168
G47 (≠ H28) to R: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity; reduced expression levels; reduced pyridoxal phosphate binding; reduced dimerization; reduced thermostability; increased propensity to aggregation; increased susceptibility to proteolytic degradation within the N-terminal region; mitochondrial mistargeting; exposure to pyridoxine can rescue the functionality by partially preventing aggregation and degradation and by redirecting all the protein to the peroxisome; dbSNP:rs180177173
G82 (= G62) to E: in HP1; abolishes alanine--glyoxylate aminotransferase activity by interfering with pyridoxal phosphate binding; dbSNP:rs121908522
W108 (≠ F88) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs180177197
A112 (≠ W92) to D: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs796052061
L150 (≠ K130) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177222
F152 (≠ V132) to I: in HP1; associated in cis with L-11 and M-340; results in protein destabilization; no loss of dimerization; decreased alanine--glyoxylate aminotransferase activity; loss of alanine--glyoxylate aminotransferase activity when associated with L-11 and M-340; mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908524
G156 (= G136) to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; dbSNP:rs121908530
S158 (≠ T139) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177225
G161 (≠ T142) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227; to R: in HP1; loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; loss of dimerization; dbSNP:rs180177227; to S: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; reduced expression levels; decreased protein stability; protein aggregation seen in the cytosol with a decreased aggregation propensity in the presence of pyridoxal phosphate; reduced peroxisomal localization; dbSNP:rs180177227
L166 (≠ V147) to P: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177230
G170 (≠ A151) to R: in HP1; associated in cis with L-11 and M-340; decrease in alanine--glyoxylate aminotransferase activity; loss of dimerization; partial loss of protein stability but protein stability increases in the presence of pyridoxal phosphate; causes protein aggregation; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; results in mitochondrial mistargeting when associated with L-11 and M-340; dbSNP:rs121908529
C173 (≠ V154) to Y: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; decreased protein stability; causes protein aggregation; dbSNP:rs180177231
D183 (= D165) to N: in HP1; loss of alanine--glyoxylate aminotransferase activity; no loss of dimerization; no effect on protein stability; dbSNP:rs180177236
S187 (= S169) to F: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization but improved dimerization in the presence of pyridoxal phosphate; decreased protein stability; dbSNP:rs180177238
I202 (= I184) to N: in HP1; uncertain significance; dbSNP:rs536352238
S205 (≠ T187) to P: in HP1; loss of alanine--glyoxylate aminotransferase activity; decreased protein stability; dbSNP:rs121908520
K209 (= K191) mutation to R: Affects pyridoxal phosphate binding; loss of alanine--glyoxylate aminotransferase activity.
S218 (= S200) to L: in HP1; loss of alanine--glyoxylate aminotransferase activity; loss of dimerization; no effect on protein stability; dbSNP:rs180177253
R233 (≠ E213) to C: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908526; to H: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs121908527
I244 (≠ L224) to T: in HP1; associated in cis with L-11 and M-340; results in protein misfolding; decreased alanine--glyoxylate aminotransferase activity in vitro; no loss of dimerization; partial mitochondrial mistargeting; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and M-340; dbSNP:rs121908525
C253 (≠ L228) to R: in HP1; associated in cis with L-11 and M-340; results in loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs180177264
I279 (≠ F255) to T: in dbSNP:rs140992177
A280 (≠ E256) to V: in dbSNP:rs73106685
V326 (≠ L299) to I: in dbSNP:rs115057148
I340 (≠ R313) to M: in allele minor; associated in cis with L-11; no effect on alanine--glyoxylate aminotransferase activity in vitro; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with L-11; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and I-152; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and R-170; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with L-11 and T-244; results in mitochondrial mistargeting when associated with L-11 and R-170; dbSNP:rs4426527

Sites not aligning to the query:

9 T → N: no loss of alanine--glyoxylate aminotransferase activity; dbSNP:rs115014558
11 P → L: in allele minor; associated in cis with M-340; decreased specific alanine--glyoxylate aminotransferase activity in vitro when associated with M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with I-152 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with R-170 and M-340; loss of alanine--glyoxylate aminotransferase activity in vitro when associated with T-244 and M-340; causes mitochondrial mistargeting when associated with R-170 and M-340; dbSNP:rs34116584

5hhyA Structure of human alanine:glyoxylate aminotransferase major allele (agt-ma) showing x-ray induced reduction of plp internal aldimine to 4'-deoxy-piridoxine-phosphate (plr) (see paper)
28% identity, 91% coverage: 4:350/382 of query aligns to 18:372/385 of 5hhyA

query
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I

L

M

L

A

E

A

M

G

T

G

K

L

P

Q

V

M

I

I

H

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

A

Q

T

I

F

M

E

D

K

E

V

I

A

K

N

E

G

G

L

I

K

Q

K

Y

V

V

F

F

S

Q

T

T

E

R

Q

N

D

P

V

L

-

T

L

L

M

V

L

I

A

S

G

G

S
|
S

G
|
G

T
x
H

A

C

A

A

M

L

E

E

A

A

V

L

V

V

N

N

T

V

L

L

N

E

P

P

G

G

D

D

N

S

V

F

L

L

V

V

I

G

N

A

A

N

G

G

K

I

F
x
W

G

G

Q

Q

R

R

W

A

R

V

D

D

I

I

C

G

K

E

T

R

Y

I

G

G

I

A

L

R

V

V

S

H

T

P

I

M

D

T

M

K

D

D

W

P

G

G

K

G

A

H

A

Y

K

T

P

L

E

Q

E

E

I

V

E

E

Q

E

F

G

L

L

K

A

A

Q

N

H

P

K

D

P

T

V

K

L

A

L

V

F

L

L

L

T

Q

H

G

G

S

-

E

E

T
x
S

S

S

T

T

T

G

V

V

Y

L

H

Q

P

P

V

L

E

D

E

G

I

F

A

G

K

E

V

L

V

C

R

H

K

R

N

Y

E

K

N

-

T

C

L

L

I

L

V

V

D
|
D

G

S

I
x
V

T

A

S

S

I

L

G

G

V

G

H

T

D

P

T

L

K

Y

F

M

D

D

E

R

W

Q

G

G

I

I

D

D

I

I

A

L

I

Y

T

S

G

G

S

S

Q
|
Q

K
|
K

A

A

F

L

M

N

L

A

P

P

G

G

L

T

S

S

L

L

I

I

C

S

L

F

S

S

K

D

K

K

A

A

W

K

N

K

-

K

-

M

F

Y

V

S

E

R

K

K

S

T

T

K

I

P

P

F

R

S

Y

F

Y

Y

L

L

D

D

L

I

R

K

-

W

-

L

-

A

-

N

-

F

K

W

E

G

L

C

K

D

S

D

Q

Q

K

P

Q

R

A

M

T
x
Y

T

H

A

H

Y
x
T

T

I

P

P

A

V

L

I

T

S

L

L

I

Y

N

S

G

-

L

L

A

R

V

E

V

S

L

L

E

A

M

L

F

I

E

A

E

E

E

Q

G

G

L

L

E

E

N

N

V

S

Y

W

K

R

R

Q

H

H

A

R

V

E

N

A

G

A

E

A

A

Y

T

L

R

H

A

G

A

R

V

L

K

Q

A

A

M

L

G

G

F

L

K

Q

L

L

L

F

A

V

E

K

T

D

P

P

S

A

-

L

-

R

-

L

N

P

A

T

A

V

T

T

G

T

F

V

Y

A

L

V

P

P

E

A

D

G

I

Y

D

D

G

W

G

R

K

D

L

I

V

V

K

S

F

Y

M

V

R

I

E

D

K

H

V

F

G

D

I

I

T

E

Y

I

A

M

G

G

Q

L

D

G

H

P

L

S

K

T

G

G

R

K

I

V

V

L

R

R

I

I

S

G

H

L

L

L

G

G

Y

C

H

N

D

A

A

T

F

R

D

E

T

N

I

V

T

D

A

R

I

V

S

T

binding (5-hydroxy-4,6-dimethylpyridin-3-yl)methyl dihydrogen phosphate: S76 (= S61), G77 (= G62), H78 (≠ T63), W103 (≠ F88), S153 (≠ T139), D178 (= D165), V180 (≠ I167), Q203 (= Q190), K204 (= K191), Y255 (≠ T235), T258 (≠ Y238)

6rv0A Human alanine:glyoxylate aminotransferase major allele (agt-ma); with pmp in the active site (see paper)
28% identity, 91% coverage: 3:350/382 of query aligns to 17:372/384 of 6rv0A

query
sites
6rv0A

K

N

K

Q

Y

L

L

L

I

L

A

G

P

P

G

G

P

P

T

S

P

N

V

L

P

P

E

P

K

R

V

I

L

M

L

A

E

A

M

G

T

G

K

L

P

Q

V

M

I

I

H

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

A

Q

T

I

F

M

E

D

K

E

V

I

A

K

N

E

G

G

L

I

K

Q

K

Y

V

V

F

F

S

Q

T

T

E

R

Q

N

D

P

V

L

-

T

L

L

M

V

L

I

A

S

G

G

S
|
S

G
|
G

T
x
H

A

C

A

A

M

L

E

E

A

A

V

L

V

V

N

N

T

V

L

L

N

E

P

P

G

G

D

D

N

S

V

F

L

L

V

V

I

G

N

A

A

N

G

G

K

I

F
x
W

G

G

Q

Q

R

R

W

A

R

V

D

D

I

I

C

G

K

E

T

R

Y

I

G

G

I

A

L

R

V

V

S

H

T

P

I

M

D

T

M

K

D

D

W

P

G

G

K

G

A

H

A

Y

K

T

P

L

E

Q

E

E

I

V

E

E

Q

E

F

G

L

L

K

A

A

Q

N

H

P

K

D

P

T

V

K

L

A

L

V

F

L

L

L

T

Q

H

G

G

S

-

E

E

T

S

S

S

T

T

T

G

V

V

Y

L

H

Q

P

P

V

L

E

D

E

G

I

F

A

G

K

E

V

L

V

C

R

H

K

R

N

Y

E

K

N

-

T

C

L

L

I

L

V

V

D
|
D

G

S

I
x
V

T
x
A

S

S

I

L

G

G

V

G

H

T

D

P

T

L

K

Y

F

M

D

D

E

R

W

Q

G

G

I

I

D

D

I

I

A

L

I

Y

T

S

G

G

S

S

Q

Q

K
|
K

A

A

F

L

M

N

L

A

P

P

G

G

L

T

S

S

L

L

I

I

C

S

L

F

S

S

K

D

K

K

A

A

W

K

N

K

-

K

-

M

F

Y

V

S

E

R

K

K

S

T

T

K

I

P

P

F

R

S

Y

F

Y

Y

L

L

D

D

L

I

R

K

-

W

-

L

-

A

-

N

-

F

K

W

E

G

L

C

K

D

S

D

Q

Q

K

P

Q

R

A

M

T

Y

T

H

A

H

Y

T

T

I

P

P

A

V

L

I

T

S

L

L

I

Y

N

S

G

-

L

L

A

R

V

E

V

S

L

L

E

A

M

L

F

I

E

A

E

E

E

Q

G

G

L

L

E

E

N

N

V

S

Y

W

K

R

R

Q

H

H

A

R

V

E

N

A

G

A

E

A

A

Y

T

L

R

H

A

G

A

R

V

L

K

Q

A

A

M

L

G

G

F

L

K

Q

L

L

L

F

A

V

E

K

T

D

P

P

S

A

-

L

-

R

-

L

N

P

A

T

A

V

T

T

G

T

F

V

Y

A

L

V

P

P

E

A

D

G

I

Y

D

D

G

W

G

R

K

D

L

I

V

V

K

S

F

Y

M

V

R

I

E

D

K

H

V

F

G

D

I

I

T

E

Y

I

A

M

G

G

Q

L

D

G

H

P

L

S

K

T

G

G

R

K

I

V

V

L

R

R

I

I

S

G

H

L

L

L

G

G

Y

C

H

N

D

A

A

T

F

R

D

E

T

N

I

V

T

D

A

R

I

V

S

T

binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S76 (= S61), G77 (= G62), H78 (≠ T63), W103 (≠ F88), D178 (= D165), V180 (≠ I167), A181 (≠ T168), K204 (= K191)

1j04A Structural mechanism of enzyme mistargeting in hereditary kidney stone disease in vitro (see paper)
28% identity, 91% coverage: 4:350/382 of query aligns to 20:374/387 of 1j04A

query
sites
1j04A

K

Q

Y

L

L

L

I

L

A

G

P
|
P

G

G

P

P

T

S

P

N

V

L

P

P

E

P

K

R

V

I

L

M

L

A

E

A

M

G

T

G

K

L

P

Q

V

M

I

I

H

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

A

Q

T

I

F

M

E

D

K

E

V

I

A

K

N

E

G

G

L

I

K

Q

K

Y

V

V

F

F

S

Q

T

T

E

R

Q

N

D

P

V

L

-

T

L

L

M

V

L

I

A

S

G

G

S

S

G

G

T

H

A

C

A

A

M

L

E

E

A

A

V

L

V

V

N

N

T

V

L

L

N

E

P

P

G

G

D

D

N

S

V

F

L

L

V

V

I

G

N

A

A

N

G

G

K

I

F

W

G

G

Q

Q

R

R

W

A

R

V

D

D

I

I

C

G

K

E

T

R

Y

I

G

G

I

A

L

R

V

V

S

H

T

P

I

M

D

T

M

K

D

D

W

P

G

G

K

G

A

H

A

Y

K

T

P

L

E

Q

E

E

I

V

E

E

Q

E

F

G

L

L

K

A

A

Q

N

H

P

K

D

P

T

V

K

L

A

L

V

F

L

L

L

T

Q

H

G

G

S

-

E

E

T

S

S

S

T

T

T

G

V

V

Y

L

H

Q

P

P

V

L

E

D

E

G

I

F

A

R

K

E

V

L

V

C

R

H

K

R

N

Y

E

K

N

-

T

C

L

L

I

L

V

V

D

D

G

S

I

V

T

A

S

S

I

L

G

G

V

G

H

T

D

P

T

L

K

Y

F

M

D

D

E

R

W

Q

G

G

I

I

D

D

I

I

A

L

I

Y

T

S

G

G

S

S

Q

Q

K

K

A

A

F

L

M

N

L

A

P

P

G

G

L

T

S

S

L

L

I

I

C

S

L

F

S

S

K

D

K

K

A

A

W

K

N

K

-

K

-

M

F

Y

V

S

E

R

K

K

S

T

T

K

I

P

P

F

R

S

Y

F

Y

Y

L

L

D

D

L

I

R

K

-

W

-

L

-

A

-

N

-

F

K

W

E

G

L

C

K

D

S

D

Q

Q

K

P

Q

R

A

M

T

Y

T

H

A

H

Y

T

T

I

P

P

A

V

L

I

T

S

L

L

I

Y

N

S

G

-

L

L

A

R

V

E

V

S

L

L

E

A

M

L

F

I

E

A

E

E

E

Q

G

G

L

L

E

E

N

N

V

S

Y

W

K

R

R

Q

H

H

A

R

V

E

N

A

G

A

E

A

A

Y

T

L

R

H

A

G

A

R

V

L

K

Q

A

A

M

L

G

G

F

L

K

Q

L

L

L

F

A

V

E

K

T

D

P

P

S

A

-

L

-

R

-

L

N

P

A

T

A

V

T

T

G

T

F

V

Y

A

L

V

P

P

E

A

D

G

I

Y

D

D

G

W

G

R

K

D

L

I

V

V

K

S

F

Y

M

V

R

I

E

D

K

H

V

F

G

D

I

I

T

E

Y

I

A

M

G

G

Q

L

D

G

H

P

L

S

K

T

G

G

R

K

I

V

V

L

R
|
R

I

I

S

G

H

L

L

L

G

G

Y

C

H

N

D

A

A

T

F

R

D

E

T

N

I

V

T

D

A

R

I

V

S

T

binding (aminooxy)acetic acid: P25 (= P9), R357 (= R333)

1h0cA The crystal structure of human alanine:glyoxylate aminotransferase (see paper)
28% identity, 91% coverage: 4:350/382 of query aligns to 20:372/385 of 1h0cA

query
sites
1h0cA

K

Q

Y

L

L

L

I

L

A

G

P
|
P

G
|
G

P

P

T

S

P

N

V

L

P

P

E

P

K

R

V

I

L

M

L

A

E

A

M

G

T

G

K

L

P

Q

V

M

I

I

H

G

H

S

R

M

T

S

S

K

E

D

F

M

S

Y

A

Q

T

I

F

M

E

D

K

E

V

I

A

K

N

E

G

G

L

I

K

Q

K

Y

V

V

F

F

S

Q

T

T

E

R

Q

N

D

P

V

L

-

T

L

L

M

V

L

I

A

S

G

G

S
|
S

G
|
G

T
x
H

A

C

A

A

M

L

E

E

A

A

V

L

V

V

N

N

T

V

L

L

N

E

P

P

G

G

D

D

N

S

V

F

L

L

V

V

I

G

N

A

A

N

G

G

K

I

F
x
W

G

G

Q

Q

R

R

W

A

R

V

D

D

I

I

C

G

K

E

T

R

Y

I

G

G

I

-

L

-

V

V

S

H

T

P

I

M

D

T

M

K

D

D

W

P

G

G

K

G

A

H

A

Y

K

T

P

L

E

Q

E

E

I

V

E

E

Q

E

F

G

L

L

K

A

A

Q

N

H

P

K

D

P

T

V

K

L

A

L

V

F

L

L

L

T

Q

H

G

G

S

-

E

E

T
x
S

S

S

T

T

T

G

V

V

Y

L

H

Q

P

P

V

L

E

D

E

G

I

F

A

G

K

E

V

L

V

C

R

H

K

R

N

Y

E

K

N

-

T

C

L

L

I

L

V

V

D
|
D

G

S

I
x
V

T

A

S

S

I

L

G

G

V

G

H

T

D

P

T

L

K

Y

F

M

D

D

E

R

W

Q

G

G

I

I

D

D

I

I

A

L

I

Y

T

S

G

G

S

S

Q

Q

K
|
K

A

A

F

L

M

N

L

A

P

P

G

G

L

T

S

S

L

L

I

I

C

S

L

F

S

S

K

D

K

K

A

A

W

K

N

K

-

K

-

M

F

Y

V

S

E

R

K

K

S

T

T

K

I

P

P

F

R

S

Y

F

Y

Y

L

L

D

D

L

I

R

K

-

W

-

L

-

A

-

N

-

F

K

W

E

G

L

C

K

D

S

D

Q

Q

K

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H

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H

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T

I

P

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A

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L

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L

L

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N

S

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-

L

L

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V

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L

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A

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G

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W

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|
R

I

I

S

G

H

L

L

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Y

C

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N

D

A

A

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N

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binding (aminooxy)acetic acid: P25 (= P9), G26 (= G10), L346 (≠ Q324), R355 (= R333)
binding pyridoxal-5'-phosphate: S78 (= S61), G79 (= G62), H80 (≠ T63), W105 (≠ F88), S153 (≠ T139), D178 (= D165), V180 (≠ I167), K204 (= K191)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 385

Query Sequence

>WP_013011685.1 NCBI__GCF_000025725.1:WP_013011685.1
MLKKYLIAPGPTPVPEKVLLEMTKPVIHHRTSEFSATFEKVANGLKKVFSTEQDVLMLAG
SGTAAMEAAVVNTLNPGDNVLVINAGKFGQRWRDICKTYGILVSTIDMDWGKAAKPEEIE
QFLKANPDTKAVLLQGSETSTTVYHPVEEIAKVVRKNENTLLIVDGITSIGVHDTKFDEW
GIDIAITGSQKAFMLPPGLSLICLSKKAWNFVEKSTIPRYYLDLRKELKSQKQATTAYTP
ALTLINGLAVVLEMFEEEGLENVYKRHAVNGEATRAAVKAMGFKLLAETPSNAATGFYLP
EDIDGGKLVKFMREKVGITYAGGQDHLKGRIVRISHLGYHDAFDTITAISGLEMGLRKFG
VDIKLGSGIAAAEEILQNYIGE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory